MD04G1081600.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009725
Physical Location & Seq
Forward (+)
11286425 .. 11286791
367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1081600.v1.1.491

Sequence Viewer

Length: 258 bp
ATGCTCCAACAGTCGATAGAAGAACTGGGTCGTGTAGATGGAGCCTCCAGCTCCAGGTTTCAGTTGAGTAATATCCAAACCTGGGTTAGTGCGGCATTTACGAACGAGGATACCTGTGAGGATGGCTTTGCCAATCTTCCTTTGAATGGCAAAGTGGAGACTACTGCTCACAGGCACGTAACAAAAGCTGCGCATCTGACAAGCAATGCCTTGGCACTCGTCAACGCCTATGCCTCAGCTAAAACTGCCTCGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

86

Amino Acids

9.06

Weight (kDa)

5.81

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 73 6.7e-16 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 192
AciI CCGC 1 cut(s) 92
AfiI CCNNNNNNNGG 3 cut(s) 54, 82, 146
AgsI TTSAA 1 cut(s) 145
AjnI CCWGG 2 cut(s) 53, 80
AluBI AGCT 3 cut(s) 51, 188, 239
AluI AGCT 3 cut(s) 51, 188, 239
Alw26I GTCTC 1 cut(s) 152
ApeKI GCWGC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 193
BbvCI CCTCAGC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 32, 116
BciT130I CCWGG 2 cut(s) 55, 82
BciVI GTATCC 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 152
BfuI GTATCC 1 cut(s) 103
BisI GCNGC 2 cut(s) 93, 189
BlsI GCNGC 2 cut(s) 94, 190
Bme1390I CCNGG 2 cut(s) 55, 82
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 2 cut(s) 55, 82
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 35
BpmI CTGGAG 2 cut(s) 31, 37
Bpu10I CCTNAGC 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 81, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 82, 146
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 2 cut(s) 55, 82
BseDI CCNNGG 2 cut(s) 81, 210
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 3 cut(s) 54, 82, 146
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 175
BslI CCNNNNNNNGG 3 cut(s) 54, 82, 146
BsmAI GTCTC 1 cut(s) 152
BspACI CCGC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 81, 210
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 55, 82
Bst4CI ACNGT 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 235, 255
BstF5I GGATG 1 cut(s) 127
BstHHI GCGC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 152
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 2 cut(s) 55, 82
BstSCI CCNGG 2 cut(s) 53, 80
BstV1I GCAGC 1 cut(s) 175
BsuI GTATCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 127
CfoI GCGC 1 cut(s) 193
CviJI RGCY 5 cut(s) 44, 51, 126, 188, 239
CviKI_1 RGCY 5 cut(s) 44, 51, 126, 188, 239
DdeI CTNAG 2 cut(s) 235, 255
Eco130I CCWWGG 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 53, 80
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaiI YATR 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 93, 189
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 2 cut(s) 93, 189
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 1 cut(s) 192
GluI GCNGC 2 cut(s) 93, 189
GsuI CTGGAG 2 cut(s) 31, 37
HhaI GCGC 1 cut(s) 193
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 223
HpyCH4III ACNGT 1 cut(s) 12
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 2 cut(s) 235, 255
HpySE526I ACGT 1 cut(s) 177
HspAI GCGC 1 cut(s) 191
LmnI GCTCC 3 cut(s) 9, 41, 56
LpnPI CCDG 8 cut(s) 11, 40, 61, 67, 67, 94, 127, 157
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 202
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 178
MboII GAAGA 2 cut(s) 32, 128
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 4 cut(s) 55, 100, 112, 244
MspR9I CCNGG 2 cut(s) 55, 82
MvaI CCWGG 2 cut(s) 55, 82
MwoI GCNNNNNNNGC 1 cut(s) 245
NlaIV GGNNCC 1 cut(s) 43
NsbI TGCGCA 1 cut(s) 192
PkrI GCNGC 2 cut(s) 94, 190
Ppu21I YACGTR 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 53, 80
PspGI CCWGG 2 cut(s) 53, 80
PspN4I GGNNCC 1 cut(s) 43
SatI GCNGC 2 cut(s) 93, 189
ScrFI CCNGG 2 cut(s) 55, 82
SetI ASST 7 cut(s) 53, 59, 83, 116, 180, 190, 241
SfaNI GCATC 1 cut(s) 202
SsiI CCGC 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 53, 80
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 12
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 14
TauI GCSGC 1 cut(s) 95
TseI GCWGC 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.