RLG00000001317

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009725
Physical Location & Seq
Reverse (-)
14006291 .. 14006878
588 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001317

Sequence Viewer

Length: 588 bp
ATGAAAGGTTATCTTACCCATATAGTTGTAGCTCCAGCAATTATTCTATTATCTAATCATATAAGCTCATGCATTGCAAACGGAGCATCTCCCGGTGTAGTAATTACCGATTACATAAAAACATCATGCAATGCCACCATGTATCCTAGGTTGTGCTGCAATGTTCTCAAAATTCACGCAGGCAAAATCGAGACGAGTCCTAATATTCTAGCTCAAACTGCCCTCGATGTGGCACTTGAATTCACCAAACAAACTCATATTATGGTGACCGATATGAAAGCTTTGAATCCCGTTGTGACAGCAGCAATGTGTGACTGTGTGGAGGAGCTTAATGTCTCTATGGAGATGCTTCAAAAGTCTAAAGTTGAATTGGGTCTCATAATTGAAGGCTCTACTTTCGGGTTACACGTTAGTAATGTTCAAACATGGGTTAGCGCTACGTTAACCAATTTAAACACCTGCAGGGATAGATTTGTCGACCTTAATCTAAATGGAAAAGTAGAGAAGATTACCGCCAGAAAAATAAATAAAGCAGCACATTTGACGAGCAATGCATTGGCATCATCAACTTATATATGTCTTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

196

Amino Acids

21.15

Weight (kDa)

8.42

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 187 1.6e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 477
AciI CCGC 1 cut(s) 513
AcsI RAATTY 2 cut(s) 171, 239
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 229
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 6 cut(s) 239, 286, 353, 368, 386, 422
AluBI AGCT 5 cut(s) 32, 66, 212, 281, 328
AluI AGCT 5 cut(s) 32, 66, 212, 281, 328
Alw26I GTCTC 3 cut(s) 185, 340, 380
Aor51HI AGCGCT 1 cut(s) 436
ApeKI GCWGC 3 cut(s) 156, 302, 533
ApoI RAATTY 2 cut(s) 171, 239
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 235, 277
AvrII CCTAGG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 572
BbvI GCAGC 3 cut(s) 143, 314, 545
BciVI GTATCC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 3 cut(s) 185, 340, 380
BfaI CTAG 2 cut(s) 147, 209
BfmI CTRYAG 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 467
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 3 cut(s) 157, 303, 534
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 3 cut(s) 158, 304, 535
Bme1390I CCNGG 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 3 cut(s) 95, 336, 569
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse3DI GCAATG 5 cut(s) 72, 136, 166, 312, 556
BseDI CCNNGG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 5 cut(s) 72, 136, 166, 312, 556
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 3 cut(s) 143, 314, 545
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 3 cut(s) 185, 340, 380
BsmBI CGTCTC 1 cut(s) 185
Bso31I GGTCTC 1 cut(s) 380
BspACI CCGC 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 5 cut(s) 72, 136, 166, 312, 556
BssECI CCNNGG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstMAI GTCTC 3 cut(s) 185, 340, 380
BstMWI GCNNNNNNNGC 2 cut(s) 83, 218
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 143, 314, 545
BstV2I GAAGAC 1 cut(s) 572
BsuI GTATCC 1 cut(s) 153
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 437
CviAII CATG 4 cut(s) 69, 126, 139, 426
CviJI RGCY 6 cut(s) 32, 66, 212, 281, 328, 390
CviKI_1 RGCY 6 cut(s) 32, 66, 212, 281, 328, 390
DraI TTTAAA 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 380
Eco47III AGCGCT 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 2 cut(s) 74, 556
ErhI CCWWGG 1 cut(s) 146
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 4 cut(s) 72, 129, 142, 429
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 4 cut(s) 68, 125, 138, 425
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 157, 303, 534
Fsp4HI GCNGC 3 cut(s) 157, 303, 534
FspBI CTAG 2 cut(s) 147, 209
GlaI GCGC 1 cut(s) 436
GluI GCNGC 3 cut(s) 157, 303, 534
GsuI CTGGAG 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 438
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 437
Hin1II CATG 4 cut(s) 72, 129, 142, 429
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 2 cut(s) 444, 478
HindII GTYRAC 2 cut(s) 444, 478
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 2 cut(s) 196, 286
HpaI GTTAAC 1 cut(s) 444
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 235, 277
Hpy166II GTNNAC 2 cut(s) 444, 478
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 2 cut(s) 444, 478
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 408, 440
HpyCH4V TGCA 6 cut(s) 72, 77, 129, 159, 462, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 218
HpySE526I ACGT 2 cut(s) 408, 440
Hsp92II CATG 4 cut(s) 72, 129, 142, 429
HspAI GCGC 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 444
LmnI GCTCC 3 cut(s) 37, 83, 325
LpnPI CCDG 6 cut(s) 48, 106, 165, 448, 472, 529
Lsp1109I GCAGC 3 cut(s) 143, 314, 545
LweI GCATC 3 cut(s) 95, 336, 569
MaeI CTAG 2 cut(s) 147, 209
MaeII ACGT 2 cut(s) 408, 440
MaeIII GTNAC 4 cut(s) 265, 295, 311, 402
MboII GAAGA 2 cut(s) 517, 572
MluCI AATT 7 cut(s) 39, 102, 171, 239, 368, 381, 448
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 2 cut(s) 233, 316
Mph1103I ATGCAT 2 cut(s) 74, 556
MseI TTAA 4 cut(s) 330, 443, 452, 483
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 83, 218
NciI CCSGG 1 cut(s) 93
NlaIII CATG 4 cut(s) 72, 129, 142, 429
NmuCI GTSAC 3 cut(s) 265, 295, 311
NsiI ATGCAT 2 cut(s) 74, 556
PaqCI CACCTGC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 3 cut(s) 158, 304, 535
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 265
PstI CTGCAG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 4 cut(s) 330, 443, 452, 483
SatI GCNGC 3 cut(s) 157, 303, 534
SbfI CCTGCAGG 1 cut(s) 464
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 93
SdaI CCTGCAGG 1 cut(s) 464
SfaNI GCATC 3 cut(s) 95, 336, 569
SfcI CTRYAG 1 cut(s) 460
Sse8387I CCTGCAGG 1 cut(s) 464
Sse9I AATT 7 cut(s) 39, 102, 171, 239, 368, 381, 448
SsiI CCGC 1 cut(s) 513
SspI AATATT 1 cut(s) 205
SspMI CTAG 2 cut(s) 147, 209
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 411, 443
TaqI TCGA 3 cut(s) 189, 225, 477
TaqII GACCGA 1 cut(s) 284
TasI AATT 7 cut(s) 39, 102, 171, 239, 368, 381, 448
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 4 cut(s) 330, 443, 452, 483
Tru9I TTAA 4 cut(s) 330, 443, 452, 483
TseFI GTSAC 3 cut(s) 265, 295, 311
TseI GCWGC 3 cut(s) 156, 302, 533
Tsp45I GTSAC 3 cut(s) 265, 295, 311
TspDTI ATGAA 2 cut(s) 17, 290
TspGWI ACGGA 1 cut(s) 96
XapI RAATTY 2 cut(s) 171, 239
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 2 cut(s) 147, 209
Zsp2I ATGCAT 2 cut(s) 74, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.