RchiOBHm_Chr7g0235051

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009725
Physical Location & Seq
Forward (+)
59741324 .. 59741935
612 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21066

Sequence Viewer

Length: 612 bp
ATGAAAGATTATCTTACGCATATAGTTGTAGCTCTAGCAGTAATTCAATTATGTAATCATATACGCTCATGCATAGCACACGGAGAATCTCCCGATCCGGGAACTACTGATTACATAAAAACATCATGTAATGCTACAATGTATCCAAGGTTGTGCTGCAATGTTCTCAAAATCCACGCGGGTAAAATCGGGATGAGTGCTAAAGTTCTATCTCAAACTGCCCTCGATATAGCCCTTGAATTCACCAAACAAACTCATATTACAGCGAAAGGCTATTCAGAAACCAAAGCCTTGAATCCCGTTGTGACATCAGCAATGCGTGACTGTGTGGAGGAGCTCAATGTCGCTGTGCAGATGCTCCAAAAGTCTAAAGTTGAGTTGGGTATGGTAATTAAAGGCTCTACTTCTGGGTTACACGTTAGTAATGTTCAAACATGGGTTAGCGCTGCATTAACCAATCTAAACACCTGCAAGGATGGATTCGTCGACCATAATCTAAATGGAACAGTAGAAAAAATTACCACCAGAAAAACTGTGAAGGCAGCACATTTGACGAGCAATGCCTTGGCACTCATCAACTTGTGTGTCTCCGCTAAAGTTACCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.85

Weight (kDa)

8.9

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 191 4.1e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 476
AccI GTMKAC 1 cut(s) 486
AccII CGCG 1 cut(s) 179
AciI CCGC 2 cut(s) 179, 591
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 239
AfeI AGCGCT 1 cut(s) 445
AfiI CCNNNNNNNGG 1 cut(s) 98
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 4 cut(s) 47, 239, 295, 431
AluBI AGCT 2 cut(s) 32, 337
AluI AGCT 2 cut(s) 32, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 592
AlwI GGATC 1 cut(s) 89
Aor51HI AGCGCT 1 cut(s) 445
ApeKI GCWGC 3 cut(s) 156, 446, 542
ApoI RAATTY 1 cut(s) 239
AspLEI GCGC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 235
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 143, 433, 554
BccI CCATC 1 cut(s) 470
BciVI GTATCC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 3 cut(s) 157, 447, 543
BlsI GCNGC 3 cut(s) 158, 448, 544
Bme1390I CCNGG 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 146, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse3DI GCAATG 3 cut(s) 166, 321, 565
BseDI CCNNGG 2 cut(s) 146, 564
BseGI GGATG 2 cut(s) 198, 481
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMI GCAATG 3 cut(s) 166, 321, 565
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 3 cut(s) 143, 433, 554
BsgI GTGCAG 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 1 cut(s) 98
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 2 cut(s) 179, 591
BspFNI CGCG 1 cut(s) 179
BspMI ACCTGC 1 cut(s) 476
BspPI GGATC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 166, 321, 565
BssECI CCNNGG 2 cut(s) 146, 564
BssMI GATC 1 cut(s) 94
BssT1I CCWWGG 2 cut(s) 146, 564
Bst4CI ACNGT 3 cut(s) 326, 508, 535
BstF5I GGATG 2 cut(s) 198, 481
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 97
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 143, 433, 554
BsuI GTATCC 1 cut(s) 153
BtsCI GGATG 2 cut(s) 198, 481
BveI ACCTGC 1 cut(s) 476
CfoI GCGC 1 cut(s) 446
CviAII CATG 3 cut(s) 69, 126, 435
CviJI RGCY 6 cut(s) 32, 233, 273, 290, 337, 399
CviKI_1 RGCY 6 cut(s) 32, 233, 273, 290, 337, 399
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 146, 564
Eco24I GRGCYC 1 cut(s) 339
Eco47III AGCGCT 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 146, 564
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 2 cut(s) 146, 564
FaeI CATG 3 cut(s) 72, 129, 438
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 3 cut(s) 68, 125, 434
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 157, 447, 543
FokI GGATG 2 cut(s) 205, 488
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 157, 447, 543
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 157, 447, 543
HaeII RGCGCY 1 cut(s) 447
HapII CCGG 1 cut(s) 98
HhaI GCGC 1 cut(s) 446
Hin1II CATG 3 cut(s) 72, 129, 438
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 3 cut(s) 86, 295, 480
HpaII CCGG 1 cut(s) 98
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 2 cut(s) 92, 190
Hpy8I GTNNAC 1 cut(s) 487
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 326, 508, 535
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 72, 159, 352, 449, 471
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 3 cut(s) 72, 129, 438
HspAI GCGC 1 cut(s) 444
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 334, 363
LpnPI CCDG 4 cut(s) 111, 393, 481, 538
Lsp1109I GCAGC 3 cut(s) 143, 433, 554
LweI GCATC 1 cut(s) 345
MaeI CTAG 1 cut(s) 35
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 4 cut(s) 304, 320, 411, 598
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 5 cut(s) 42, 47, 239, 390, 516
MnlI CCTC 2 cut(s) 233, 325
Mph1103I ATGCAT 1 cut(s) 74
MseI TTAA 2 cut(s) 393, 452
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 179
NciI CCSGG 1 cut(s) 99
NdeII GATC 1 cut(s) 94
NlaIII CATG 3 cut(s) 72, 129, 438
NmuCI GTSAC 2 cut(s) 304, 320
NsiI ATGCAT 1 cut(s) 74
PaqCI CACCTGC 1 cut(s) 476
PfeI GAWTC 3 cut(s) 86, 295, 480
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 3 cut(s) 158, 448, 544
Psp124BI GAGCTC 1 cut(s) 339
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 485
SaqAI TTAA 2 cut(s) 393, 452
SatI GCNGC 3 cut(s) 157, 447, 543
Sau3AI GATC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 339
SetI ASST 6 cut(s) 34, 152, 339, 420, 470, 605
SfaNI GCATC 1 cut(s) 345
Sse9I AATT 5 cut(s) 42, 47, 239, 390, 516
SsiI CCGC 2 cut(s) 179, 591
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 2 cut(s) 146, 564
TaaI ACNGT 3 cut(s) 326, 508, 535
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 225, 486
TasI AATT 5 cut(s) 42, 47, 239, 390, 516
TfiI GAWTC 3 cut(s) 86, 295, 480
Tru1I TTAA 2 cut(s) 393, 452
Tru9I TTAA 2 cut(s) 393, 452
TseFI GTSAC 2 cut(s) 304, 320
TseI GCWGC 3 cut(s) 156, 446, 542
Tsp45I GTSAC 2 cut(s) 304, 320
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 96
XapI RAATTY 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.