Rroxscaffold_3G00228030

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009725
Physical Location & Seq
Reverse (-)
12343708 .. 12344319
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228030.1

Sequence Viewer

Length: 612 bp
ATGAAAGGTTATCTTACCCATATAGCTGTAGCTCTAGCAATTATTCAATTATCTAATCATATAAGCTCATGCACTGCACACAGAGCATCTCCCGGTGTAGTAACTACTGATTACATAAAAACATCATGCAATGCCACCATGTATCCTAGGTTGTGCTGCAATGTTCTCAAAATTCACACAGGCAAAATCGAGACGAGTCTTAATATTCTAGCTCAAACTGCCCTCGATGTAGCACTTGAATTCATCAAACAAACTCATATTATGGTGAAAAGCTATTCAAATATCAAAGCTTTGTATCCTGTTGTGACAGCAGCAATGCGTGATTGTGTGGAGGAGCTTAATGTCGCTATGGAGATGCTTGAAAAGTCTAAAGTTGAGTTGGGTCTCATAATTGAAGGCTCTACTTTCGGGTTACACGTTTGTAATATTCAAACATGGGTTAGCGCTGCGTTAACCAATTTAAACACCTGCAAAGATGGATTTGTTGACCTTAATCTAAATGGAAAAGTAGAGAAGATTACCGCCAGAAAAATTGTAAAAGCAGCACATTTGACGAGCAATGCATTGGCACTCATCAACTTATGTGTCTCCGCTAAAGTTTCCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

22.01

Weight (kDa)

8.76

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 191 3.2e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 476
AciI CCGC 2 cut(s) 522, 591
AcsI RAATTY 2 cut(s) 171, 239
AfeI AGCGCT 1 cut(s) 445
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 6 cut(s) 47, 239, 279, 362, 395, 431
AluBI AGCT 7 cut(s) 26, 32, 66, 212, 273, 290, 337
AluI AGCT 7 cut(s) 26, 32, 66, 212, 273, 290, 337
Alw26I GTCTC 3 cut(s) 185, 389, 592
Aor51HI AGCGCT 1 cut(s) 445
ApeKI GCWGC 4 cut(s) 156, 311, 446, 542
ApoI RAATTY 2 cut(s) 171, 239
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 146
BbvI GCAGC 4 cut(s) 143, 323, 433, 554
BccI CCATC 1 cut(s) 470
BciVI GTATCC 2 cut(s) 153, 306
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 3 cut(s) 185, 389, 592
BfaI CTAG 3 cut(s) 35, 147, 209
BfmI CTRYAG 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 2 cut(s) 153, 306
BisI GCNGC 4 cut(s) 157, 312, 447, 543
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 4 cut(s) 158, 313, 448, 544
Bme1390I CCNGG 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 95, 345
BpuMI CCSGG 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 146
Bse3DI GCAATG 4 cut(s) 136, 166, 321, 565
BseDI CCNNGG 1 cut(s) 146
BseMI GCAATG 4 cut(s) 136, 166, 321, 565
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 4 cut(s) 143, 323, 433, 554
BsgI GTGCAG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 93
BsmAI GTCTC 3 cut(s) 185, 389, 592
BsmBI CGTCTC 1 cut(s) 185
Bso31I GGTCTC 1 cut(s) 389
BspACI CCGC 2 cut(s) 522, 591
BspMI ACCTGC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 4 cut(s) 136, 166, 321, 565
BssECI CCNNGG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstMAI GTCTC 3 cut(s) 185, 389, 592
BstMWI GCNNNNNNNGC 2 cut(s) 83, 218
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 4 cut(s) 143, 323, 433, 554
BsuI GTATCC 2 cut(s) 153, 306
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
BveI ACCTGC 1 cut(s) 476
CfoI GCGC 1 cut(s) 446
CviAII CATG 4 cut(s) 69, 126, 139, 435
CviJI RGCY 8 cut(s) 26, 32, 66, 212, 273, 290, 337, 399
CviKI_1 RGCY 8 cut(s) 26, 32, 66, 212, 273, 290, 337, 399
DraI TTTAAA 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 389
Eco47III AGCGCT 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 146
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 4 cut(s) 72, 129, 142, 438
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 4 cut(s) 68, 125, 138, 434
Fnu4HI GCNGC 4 cut(s) 157, 312, 447, 543
Fsp4HI GCNGC 4 cut(s) 157, 312, 447, 543
FspBI CTAG 3 cut(s) 35, 147, 209
GlaI GCGC 1 cut(s) 445
GluI GCNGC 4 cut(s) 157, 312, 447, 543
HaeII RGCGCY 1 cut(s) 447
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 446
Hin1II CATG 4 cut(s) 72, 129, 142, 438
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 453, 487
HindII GTYRAC 2 cut(s) 453, 487
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 1 cut(s) 196
HpaI GTTAAC 1 cut(s) 453
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 2 cut(s) 453, 487
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 2 cut(s) 453, 487
HpyAV CCTTC 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 72, 77, 129, 159, 471, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 218
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 4 cut(s) 72, 129, 142, 438
HspAI GCGC 1 cut(s) 444
KspAI GTTAAC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 334
LpnPI CCDG 5 cut(s) 106, 165, 312, 481, 538
Lsp1109I GCAGC 4 cut(s) 143, 323, 433, 554
LweI GCATC 2 cut(s) 95, 345
MaeI CTAG 3 cut(s) 35, 147, 209
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 100, 304, 411
MboII GAAGA 1 cut(s) 526
MluCI AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 2 cut(s) 233, 325
Mph1103I ATGCAT 1 cut(s) 565
MseI TTAA 5 cut(s) 201, 339, 452, 461, 492
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 83, 218
NciI CCSGG 1 cut(s) 93
NlaIII CATG 4 cut(s) 72, 129, 142, 438
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 565
PaqCI CACCTGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PkrI GCNGC 4 cut(s) 158, 313, 448, 544
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
SaqAI TTAA 5 cut(s) 201, 339, 452, 461, 492
SatI GCNGC 4 cut(s) 157, 312, 447, 543
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 93
SfaNI GCATC 2 cut(s) 95, 345
SfcI CTRYAG 1 cut(s) 27
Sse9I AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
SsiI CCGC 2 cut(s) 522, 591
SspI AATATT 2 cut(s) 205, 427
SspMI CTAG 3 cut(s) 35, 147, 209
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 189, 225
TasI AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
Tru1I TTAA 5 cut(s) 201, 339, 452, 461, 492
Tru9I TTAA 5 cut(s) 201, 339, 452, 461, 492
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 156, 311, 446, 542
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 17, 232
TspRI CASTG 1 cut(s) 79
XapI RAATTY 2 cut(s) 171, 239
XmaJI CCTAGG 1 cut(s) 146
XspI CTAG 3 cut(s) 35, 147, 209
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.