Rroxscaffold_3G00233430

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009725
Physical Location & Seq
Forward (+)
19406832 .. 19409416
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233430.1

Sequence Viewer

Length: 594 bp
ATGAAGTGCCTCCGACCGGAGGAGTGGAAGATTAAGCGCCGGTGGAGGAGGAGGCGGTGGAGGAGGGATGTACAGCTCGCAACACCGCCGCCGGAGATGGAGAGATTAAGCGGAGGGATGTTTGGGTTCGATCGGATCGCACCGAGGAGGATACAAGGCTGGGAAATCCAAACCAACCCTAAAGTACTAGCTCATACTGCGCTCAGCGTGGCTCTTGAATTTGCCAAACAAACTCATGTGACAGTGAAAATGTTTTCAACATTTCAAAGTTTGGGTCCTCTGGTGAGACCAGCAATCTCTGATTGCGTGAAGCAACTAGATATTGCTATGGAGGAGCTCCAAAATTCTAAAGATGAACTGAGTAAAATAGTTCAAGTCTCTAATTCTGGATTTCAAATAAGCAATATTCAAACATGGGTTAGTGCTGCACTGACAAATGAGAACACTTGCATGGATGGCTTTGGCGAGATCACTCTAAAGGGAAAAGTGGAGACTATTTCTTATAGGCACATAGCTAAAGTCACACAATTAACGAGCAATGCCCTGGCACTCTTCAATAGTTATGCAGTATCATCTAAAGTTTCTTTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

197

Amino Acids

22.42

Weight (kDa)

9.86

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 60 - 184 4.6e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 55, 86, 89, 111
AclWI GGATC 1 cut(s) 143
AcsI RAATTY 2 cut(s) 218, 343
AfaI GTAC 2 cut(s) 72, 186
AfiI CCNNNNNNNGG 2 cut(s) 16, 19
AgsI TTSAA 7 cut(s) 218, 258, 266, 374, 395, 410, 556
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 4 cut(s) 76, 191, 337, 515
AluI AGCT 4 cut(s) 76, 191, 337, 515
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 3 cut(s) 280, 382, 485
AlwI GGATC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 2 cut(s) 218, 343
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AspLEI GCGC 2 cut(s) 39, 202
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 91, 449
BciT130I CCWGG 1 cut(s) 545
BciVI GTATCC 1 cut(s) 144
BcoDI GTCTC 3 cut(s) 280, 382, 485
BfaI CTAG 2 cut(s) 188, 317
BfoI RGCGCY 1 cut(s) 40
BfuI GTATCC 1 cut(s) 144
BisI GCNGC 2 cut(s) 89, 426
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 2 cut(s) 90, 427
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 545
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 143, 543
BsaWI WCCGGW 1 cut(s) 16
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 19
Bse118I RCCGGY 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 544
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 143, 543
BseGI GGATG 3 cut(s) 73, 123, 460
BseLI CCNNNNNNNGG 2 cut(s) 16, 19
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 2 cut(s) 217, 350
BseRI GAGGAG 6 cut(s) 35, 61, 64, 76, 160, 347
BseXI GCAGC 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 411
Bsh1285I CGRYCG 2 cut(s) 17, 133
BsiEI CGRYCG 2 cut(s) 17, 133
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 3 cut(s) 17, 40, 92
BslI CCNNNNNNNGG 2 cut(s) 16, 19
BsmAI GTCTC 3 cut(s) 280, 382, 485
Bso31I GGTCTC 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 3 cut(s) 130, 135, 468
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 4 cut(s) 55, 86, 89, 111
BspCNI CTCAG 2 cut(s) 216, 351
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 544
BsrFI RCCGGY 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 70
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 2 cut(s) 143, 543
BssMI GATC 3 cut(s) 130, 135, 468
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 2 cut(s) 203, 359
BstF5I GGATG 3 cut(s) 73, 123, 460
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 2 cut(s) 39, 202
BstKTI GATC 3 cut(s) 133, 138, 471
BstMAI GTCTC 3 cut(s) 280, 382, 485
BstMBI GATC 3 cut(s) 130, 135, 468
BstMCI CGRYCG 2 cut(s) 17, 133
BstMWI GCNNNNNNNGC 2 cut(s) 197, 456
BstNI CCWGG 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 412
BsuI GTATCC 1 cut(s) 144
BtsCI GGATG 3 cut(s) 73, 123, 460
BtsIMutI CAGTG 2 cut(s) 249, 428
Cac8I GCNNGC 1 cut(s) 78
CfoI GCGC 2 cut(s) 39, 202
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 71, 185
CviAII CATG 3 cut(s) 236, 414, 451
CviJI RGCY 7 cut(s) 76, 159, 191, 212, 337, 459, 515
CviKI_1 RGCY 7 cut(s) 76, 159, 191, 212, 337, 459, 515
CviQI GTAC 2 cut(s) 71, 185
DdeI CTNAG 2 cut(s) 203, 359
DpnI GATC 3 cut(s) 132, 137, 470
DpnII GATC 3 cut(s) 130, 135, 468
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 339
FaeI CATG 3 cut(s) 239, 417, 454
FaiI YATR 8 cut(s) 195, 237, 329, 415, 452, 504, 512, 564
FatI CATG 3 cut(s) 235, 413, 450
Fnu4HI GCNGC 2 cut(s) 89, 426
FokI GGATG 3 cut(s) 80, 130, 467
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 2 cut(s) 89, 426
FspBI CTAG 2 cut(s) 188, 317
GlaI GCGC 2 cut(s) 38, 201
GluI GCNGC 2 cut(s) 89, 426
GsaI CCCAGC 1 cut(s) 163
HaeII RGCGCY 1 cut(s) 40
HapII CCGG 3 cut(s) 17, 40, 92
HhaI GCGC 2 cut(s) 39, 202
Hin1II CATG 3 cut(s) 239, 417, 454
Hin6I GCGC 2 cut(s) 37, 200
HinP1I GCGC 2 cut(s) 37, 200
HpaII CCGG 3 cut(s) 17, 40, 92
HphI GGTGA 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 14, 135, 301
Hpy188III TCNNGA 2 cut(s) 215, 387
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 4 cut(s) 428, 450, 566, 589
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 456
HpyF3I CTNAG 2 cut(s) 203, 359
Hsp92II CATG 3 cut(s) 239, 417, 454
HspAI GCGC 2 cut(s) 37, 200
Kzo9I GATC 3 cut(s) 130, 135, 468
LmnI GCTCC 2 cut(s) 334, 342
LpnPI CCDG 9 cut(s) 30, 53, 105, 145, 266, 303, 372, 530, 557
Lsp1109I GCAGC 1 cut(s) 412
MaeI CTAG 2 cut(s) 188, 317
MaeIII GTNAC 2 cut(s) 238, 520
MalI GATC 3 cut(s) 132, 137, 470
MboI GATC 3 cut(s) 130, 135, 468
MboII GAAGA 2 cut(s) 40, 544
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 4 cut(s) 218, 343, 382, 527
MmeI TCCRAC 1 cut(s) 37
MseI TTAA 4 cut(s) 33, 107, 530, 592
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 3 cut(s) 17, 40, 92
MspR9I CCNGG 1 cut(s) 545
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 197, 456
NdeII GATC 3 cut(s) 130, 135, 468
NlaIII CATG 3 cut(s) 239, 417, 454
NlaIV GGNNCC 1 cut(s) 276
NmuCI GTSAC 2 cut(s) 238, 520
PkrI GCNGC 2 cut(s) 90, 427
Ple19I CGATCG 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 275
PspPPI RGGWCCY 1 cut(s) 275
PvuI CGATCG 1 cut(s) 133
RsaI GTAC 2 cut(s) 72, 186
RsaNI GTAC 2 cut(s) 71, 185
RseI CAYNNNNRTG 1 cut(s) 449
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 4 cut(s) 33, 107, 530, 592
SatI GCNGC 2 cut(s) 89, 426
Sau3AI GATC 3 cut(s) 130, 135, 468
Sau96I GGNCC 1 cut(s) 275
ScaI AGTACT 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 545
SduI GDGCHC 1 cut(s) 339
SetI ASST 4 cut(s) 78, 193, 339, 517
SgrAI CRCCGGYG 1 cut(s) 39
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 4 cut(s) 218, 343, 382, 527
SsiI CCGC 4 cut(s) 55, 86, 89, 111
SspI AATATT 1 cut(s) 406
SspMI CTAG 2 cut(s) 188, 317
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 129
TasI AATT 4 cut(s) 218, 343, 382, 527
TatI WGTACW 2 cut(s) 70, 184
TauI GCSGC 1 cut(s) 91
Tru1I TTAA 4 cut(s) 33, 107, 530, 592
Tru9I TTAA 4 cut(s) 33, 107, 530, 592
TscAI CASTG 2 cut(s) 249, 435
TseFI GTSAC 2 cut(s) 238, 520
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 2 cut(s) 238, 520
TspDTI ATGAA 2 cut(s) 17, 369
TspRI CASTG 2 cut(s) 249, 435
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 2 cut(s) 218, 343
XspI CTAG 2 cut(s) 188, 317
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.