Rroxscaffold_3G00226350

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009725
Physical Location & Seq
Reverse (-)
9832823 .. 9833415
593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226350.1

Sequence Viewer

Length: 345 bp
ATGCGTGACTGTGTGGAGGAGCTCAATGTCGCTGTGCAGATGCTCCAAAAGTCTAAAGTTGAGTTGGGTATGGTAATTAAAGGCTCTATTTCTGGGTTACACGTCAGTAATGTTCAAACATGGGTTAGCGCTGCATTAACCAATCTAAACACCTGCAAGGATGGATTCGTCGACCATAATCTAAATGGAACAGTAGAAAAAATTACCACCAGAAAAACTGTAAAGGCAGCACATTTGACGAGCAATACCTTGGCACTCATCAACTTTGGGTACCCTAGCAGATTGAAGGAGGGAGACCTCTCTTTCTTGGGCCATATACCTAGAGTGAGACACTATCGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

114

Amino Acids

12.68

Weight (kDa)

9.51

Isoelectric Point (pI)

12.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 1 - 86 3.8e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
Acc65I GGTACC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 270
AccI GTMKAC 1 cut(s) 171
AfaI GTAC 1 cut(s) 272
AfeI AGCGCT 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 2 cut(s) 116, 286
AjiI CACGTC 1 cut(s) 103
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 288, 322
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 131, 227
Asp718I GGTACC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 118, 239
BccI CCATC 1 cut(s) 155
BcoDI GTCTC 2 cut(s) 288, 322
BfaI CTAG 2 cut(s) 276, 321
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 2 cut(s) 132, 228
BlsI GCNGC 2 cut(s) 133, 229
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 272
BmsI GCATC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 118, 239
BsgI GTGCAG 1 cut(s) 56
BshFI GGCC 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 24
BsmAI GTCTC 2 cut(s) 288, 322
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 24
BspANI GGCC 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 272
BspMI ACCTGC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 11, 193, 220
BstF5I GGATG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstMAI GTCTC 2 cut(s) 288, 322
BstV1I GCAGC 2 cut(s) 118, 239
BsuRI GGCC 1 cut(s) 312
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 166
BveI ACCTGC 1 cut(s) 161
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 120
CviJI RGCY 3 cut(s) 22, 84, 312
CviKI_1 RGCY 3 cut(s) 22, 84, 312
CviQI GTAC 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 288
Eco47III AGCGCT 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 123
FaiI YATR 5 cut(s) 71, 121, 177, 315, 317
FatI CATG 1 cut(s) 119
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 132, 228
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 132, 228
FspBI CTAG 2 cut(s) 276, 321
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 132, 228
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 312
HhaI GCGC 1 cut(s) 131
Hin1II CATG 1 cut(s) 123
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 165
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 344
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 3 cut(s) 11, 193, 220
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 37, 134, 156
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 1 cut(s) 123
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 274
LmnI GCTCC 2 cut(s) 19, 48
LpnPI CCDG 3 cut(s) 78, 166, 223
Lsp1109I GCAGC 2 cut(s) 118, 239
LweI GCATC 1 cut(s) 30
MaeI CTAG 2 cut(s) 276, 321
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 5, 96
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 2 cut(s) 75, 201
MnlI CCTC 3 cut(s) 10, 283, 308
MseI TTAA 2 cut(s) 78, 137
NlaIII CATG 1 cut(s) 123
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 5
PaqCI CACCTGC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 165
PkrI GCNGC 2 cut(s) 133, 229
Psp124BI GAGCTC 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 310
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SacI GAGCTC 1 cut(s) 24
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 78, 137
SatI GCNGC 2 cut(s) 132, 228
Sau96I GGNCC 1 cut(s) 310
SduI GDGCHC 1 cut(s) 24
SetI ASST 6 cut(s) 24, 105, 155, 251, 300, 322
SfaNI GCATC 1 cut(s) 30
Sse9I AATT 2 cut(s) 75, 201
SspMI CTAG 2 cut(s) 276, 321
SstI GAGCTC 1 cut(s) 24
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 3 cut(s) 11, 193, 220
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 171
TasI AATT 2 cut(s) 75, 201
TfiI GAWTC 1 cut(s) 165
Tru1I TTAA 2 cut(s) 78, 137
Tru9I TTAA 2 cut(s) 78, 137
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 2 cut(s) 131, 227
Tsp45I GTSAC 1 cut(s) 5
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 276, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.