Rh7AG430300

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009725
Physical Location & Seq
Forward (+)
57893070 .. 57897760
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG430300.1

Sequence Viewer

Length: 612 bp
ATGAAAGGTTATCTTACCCATATAGTTGTAGCTCTAGCAATTATTCAATTATCTAATCATATAAGCTCATGCACTGCACATAGAGCATCTCCCGGTGTAGTAACTACTGATTACATAAAAACATCATGCAATGCCACCATGTATCCTAGGTTGTGCTGCAATGTTCTCAAAATTCACGCAGGCAAAATCGAGATGAGTCCTAATATTCTAGCTCAAACTGCCCTCGATGTGGCACTTGAATTCACCAAACAAACTCATATTATGGTGGAAAGCTATTCAAATATCAAAGCTTTGAATCCTGTTGTGATAGCAGCAATGCGTGACTGTGTGGAGGAGCTTAATGTCGCTATGGAGATGCTTCAAAAGTCTAAAGTTGAGTTGGGTCTCATAATTGAAGGCTCTACTTTCGGGTTACACGTTAGTAATCTTCAAACATGGGTTAGCGCTGCGTTAACCAATTTAAACACCTGCAAGGATGGATTTGTCGACCTTAATATAAATGGAAAAGTAGAGAAGATTATCGCCAGAAAAATTGTAAAAGCAGCACATTTGACGAGCAATGCATTGGCACTCATCAACTTATGTGTGTCCGCTAAAGTTTCCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.97

Weight (kDa)

8.67

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 191 5.3e-31 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 476
AccI GTMKAC 1 cut(s) 486
AciI CCGC 1 cut(s) 591
AcsI RAATTY 2 cut(s) 171, 239
AfeI AGCGCT 1 cut(s) 445
AfiI CCNNNNNNNGG 1 cut(s) 229
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 7 cut(s) 47, 239, 279, 295, 362, 395, 431
AluBI AGCT 6 cut(s) 32, 66, 212, 273, 290, 337
AluI AGCT 6 cut(s) 32, 66, 212, 273, 290, 337
Alw26I GTCTC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 445
ApeKI GCWGC 4 cut(s) 156, 311, 446, 542
ApoI RAATTY 2 cut(s) 171, 239
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 235
AvrII CCTAGG 1 cut(s) 146
BbvI GCAGC 4 cut(s) 143, 323, 433, 554
BccI CCATC 1 cut(s) 470
BciVI GTATCC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 3 cut(s) 35, 147, 209
BfoI RGCGCY 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 4 cut(s) 157, 312, 447, 543
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 4 cut(s) 158, 313, 448, 544
Bme1390I CCNGG 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 95, 345
BpuMI CCSGG 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse3DI GCAATG 4 cut(s) 136, 166, 321, 565
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 4 cut(s) 136, 166, 321, 565
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 4 cut(s) 143, 323, 433, 554
BsgI GTGCAG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 389
BspACI CCGC 1 cut(s) 591
BspMI ACCTGC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 4 cut(s) 136, 166, 321, 565
BssECI CCNNGG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 181
BstF5I GGATG 1 cut(s) 481
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstMAI GTCTC 1 cut(s) 389
BstMWI GCNNNNNNNGC 2 cut(s) 83, 218
BstSCI CCNGG 1 cut(s) 91
BstV1I GCAGC 4 cut(s) 143, 323, 433, 554
BsuI GTATCC 1 cut(s) 153
BtsCI GGATG 1 cut(s) 481
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 446
CviAII CATG 4 cut(s) 69, 126, 139, 435
CviJI RGCY 7 cut(s) 32, 66, 212, 273, 290, 337, 399
CviKI_1 RGCY 7 cut(s) 32, 66, 212, 273, 290, 337, 399
DraI TTTAAA 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 389
Eco47III AGCGCT 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 72, 129, 142, 438
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 4 cut(s) 68, 125, 138, 434
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 157, 312, 447, 543
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 157, 312, 447, 543
FspBI CTAG 3 cut(s) 35, 147, 209
GlaI GCGC 1 cut(s) 445
GluI GCNGC 4 cut(s) 157, 312, 447, 543
HaeII RGCGCY 1 cut(s) 447
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 446
Hin1II CATG 4 cut(s) 72, 129, 142, 438
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 453, 487
HindII GTYRAC 2 cut(s) 453, 487
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 2 cut(s) 196, 295
HpaI GTTAAC 1 cut(s) 453
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 453, 487
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 2 cut(s) 453, 487
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 72, 77, 129, 159, 471, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 218
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 4 cut(s) 72, 129, 142, 438
HspAI GCGC 1 cut(s) 444
KspAI GTTAAC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 334
LpnPI CCDG 5 cut(s) 106, 165, 312, 481, 538
Lsp1109I GCAGC 4 cut(s) 143, 323, 433, 554
LweI GCATC 2 cut(s) 95, 345
MaeI CTAG 3 cut(s) 35, 147, 209
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 100, 320, 411
MboII GAAGA 2 cut(s) 419, 526
MluCI AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 2 cut(s) 233, 325
Mph1103I ATGCAT 1 cut(s) 565
MseI TTAA 4 cut(s) 339, 452, 461, 492
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 83, 218
NciI CCSGG 1 cut(s) 93
NlaIII CATG 4 cut(s) 72, 129, 142, 438
NmuCI GTSAC 1 cut(s) 320
NsiI ATGCAT 1 cut(s) 565
PaqCI CACCTGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 4 cut(s) 158, 313, 448, 544
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
SalI GTCGAC 1 cut(s) 485
SaqAI TTAA 4 cut(s) 339, 452, 461, 492
SatI GCNGC 4 cut(s) 157, 312, 447, 543
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 93
SfaNI GCATC 2 cut(s) 95, 345
Sse9I AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
SsiI CCGC 1 cut(s) 591
SspI AATATT 1 cut(s) 205
SspMI CTAG 3 cut(s) 35, 147, 209
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 189, 225, 486
TasI AATT 7 cut(s) 39, 47, 171, 239, 390, 457, 531
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 4 cut(s) 339, 452, 461, 492
Tru9I TTAA 4 cut(s) 339, 452, 461, 492
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 4 cut(s) 156, 311, 446, 542
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 79
XapI RAATTY 2 cut(s) 171, 239
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 3 cut(s) 35, 147, 209
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.