RchiOBHm_Chr7g0232681

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009725
Physical Location & Seq
Forward (+)
56875633 .. 56875905
273 bp
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UTR
Exon/CDS
Intron
PRQ20856

Sequence Viewer

Length: 273 bp
ATGTCGCTATGGAGATGTTTCAAAAGTCTAAAAGTTGAGTTGGGTCTCATAATTGAAGGCTCTACTTTCGGGTTACACGTTAGTAATCTTCAAACATGGGTTAGCGCTGCGTTAACCAATTTAAACACATGCAAGGATGGATTTGTCGACCTTAATCTAAATGGAAAAGTAGAGAAGATTATCGCCAGAAAAATTGTAAAAGCAGCACATTTGACGAGCAATGCATTGGCACTCATCAACTTATGTGTGTCCGCTAAAGTTTCCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

90

Amino Acids

9.75

Weight (kDa)

9.46

Isoelectric Point (pI)

9.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 20 - 78 2.7e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 147
AciI CCGC 1 cut(s) 252
AfeI AGCGCT 1 cut(s) 106
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 3 cut(s) 22, 56, 92
Alw26I GTCTC 1 cut(s) 50
Aor51HI AGCGCT 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 107, 203
AspLEI GCGC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 94, 215
BccI CCATC 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 2 cut(s) 108, 204
BlsI GCNGC 2 cut(s) 109, 205
BsaI GGTCTC 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 226
BseGI GGATG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 94, 215
BsmAI GTCTC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 50
BspACI CCGC 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 142
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstMAI GTCTC 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 94, 215
BtsCI GGATG 1 cut(s) 142
CfoI GCGC 1 cut(s) 107
CviAII CATG 2 cut(s) 96, 129
CviJI RGCY 1 cut(s) 60
CviKI_1 RGCY 1 cut(s) 60
DraI TTTAAA 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 50
Eco47III AGCGCT 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 226
FaeI CATG 2 cut(s) 99, 132
FaiI YATR 5 cut(s) 10, 50, 97, 130, 244
FatI CATG 2 cut(s) 95, 128
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 108, 204
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 108, 204
GlaI GCGC 1 cut(s) 106
GluI GCNGC 2 cut(s) 108, 204
HaeII RGCGCY 1 cut(s) 108
HhaI GCGC 1 cut(s) 107
Hin1II CATG 2 cut(s) 99, 132
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 2 cut(s) 114, 148
HindII GTYRAC 2 cut(s) 114, 148
HpaI GTTAAC 1 cut(s) 114
Hpy166II GTNNAC 2 cut(s) 114, 148
Hpy8I GTNNAC 2 cut(s) 114, 148
HpyAV CCTTC 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 2 cut(s) 132, 224
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 2 cut(s) 99, 132
HspAI GCGC 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 114
LpnPI CCDG 1 cut(s) 199
Lsp1109I GCAGC 2 cut(s) 94, 215
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 72
MboII GAAGA 2 cut(s) 80, 187
MluCI AATT 3 cut(s) 51, 118, 192
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 3 cut(s) 113, 122, 153
NlaIII CATG 2 cut(s) 99, 132
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PkrI GCNGC 2 cut(s) 109, 205
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 3 cut(s) 113, 122, 153
SatI GCNGC 2 cut(s) 108, 204
SetI ASST 2 cut(s) 81, 153
SgeI CNNG 7 cut(s) 82, 89, 108, 141, 145, 198, 228
Sse9I AATT 3 cut(s) 51, 118, 192
SsiI CCGC 1 cut(s) 252
TaiI ACGT 1 cut(s) 81
TaqI TCGA 1 cut(s) 147
TasI AATT 3 cut(s) 51, 118, 192
Tru1I TTAA 3 cut(s) 113, 122, 153
Tru9I TTAA 3 cut(s) 113, 122, 153
TseI GCWGC 2 cut(s) 107, 203
XceI RCATGY 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.