Rw7G035550

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009725
Physical Location & Seq
Forward (+)
53253160 .. 53253510
351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035550.1

Sequence Viewer

Length: 351 bp
ATGGTGAAAAGCTATTCAAATATCAAAGCTACGAATCCTGTTGTGACAGCAGCAATGTGTGACTGTGTGGAAGAGCTTAATGTCGCTATGGAGATGCTTCAAAAGTCTAAAGTTGAGTTGGGTCTCATAATTGAAGGCTCTACTTTCGGGTTACACGTTAGTAATCTTCAAACATGGGTTAGCGCTGCGTTAACCAATTTAAACACCTGCAAGGATGGATTTGTAGACCTTAATCTAAATGGAAAAGTAGAGAAGATTACCGCCAGAAAACTTGTAAAAGCAGCACATTTGACGAGCAATGCATTGGCACTCATCAACTTATGTGTGTCCGCTAAAGTTTCCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

116

Amino Acids

12.42

Weight (kDa)

8.39

Isoelectric Point (pI)

15.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 12 - 104 4e-19 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 215
AccI GTMKAC 1 cut(s) 225
AciI CCGC 2 cut(s) 261, 330
AfeI AGCGCT 1 cut(s) 184
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 4 cut(s) 18, 101, 134, 170
AluBI AGCT 3 cut(s) 12, 29, 76
AluI AGCT 3 cut(s) 12, 29, 76
Alw26I GTCTC 1 cut(s) 128
Aor51HI AGCGCT 1 cut(s) 184
ApeKI GCWGC 3 cut(s) 50, 185, 281
AspLEI GCGC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 16
BbvI GCAGC 3 cut(s) 62, 172, 293
BccI CCATC 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 215
BisI GCNGC 3 cut(s) 51, 186, 282
BlsI GCNGC 3 cut(s) 52, 187, 283
BmsI GCATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 128
Bse3DI GCAATG 2 cut(s) 60, 304
BseGI GGATG 1 cut(s) 220
BseMI GCAATG 2 cut(s) 60, 304
BseXI GCAGC 3 cut(s) 62, 172, 293
BsmAI GTCTC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 128
BspACI CCGC 2 cut(s) 261, 330
BspMI ACCTGC 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 66
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 60, 304
Bst4CI ACNGT 1 cut(s) 65
Bst6I CTCTTC 1 cut(s) 66
BstF5I GGATG 1 cut(s) 220
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstMAI GTCTC 1 cut(s) 128
BstV1I GCAGC 3 cut(s) 62, 172, 293
BtsCI GGATG 1 cut(s) 220
BveI ACCTGC 1 cut(s) 215
CfoI GCGC 1 cut(s) 185
CviAII CATG 1 cut(s) 174
CviJI RGCY 4 cut(s) 12, 29, 76, 138
CviKI_1 RGCY 4 cut(s) 12, 29, 76, 138
DraI TTTAAA 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 128
Eco47III AGCGCT 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 304
FaeI CATG 1 cut(s) 177
FaiI YATR 4 cut(s) 89, 128, 175, 322
FatI CATG 1 cut(s) 173
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 51, 186, 282
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 51, 186, 282
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 51, 186, 282
HaeII RGCGCY 1 cut(s) 186
HhaI GCGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 177
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 1 cut(s) 34
HpaI GTTAAC 1 cut(s) 192
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 192, 226
Hpy8I GTNNAC 2 cut(s) 192, 226
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 2 cut(s) 210, 302
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 1 cut(s) 177
HspAI GCGC 1 cut(s) 183
KspAI GTTAAC 1 cut(s) 192
LguI GCTCTTC 1 cut(s) 66
LpnPI CCDG 3 cut(s) 51, 220, 277
Lsp1109I GCAGC 3 cut(s) 62, 172, 293
LweI GCATC 1 cut(s) 84
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 3 cut(s) 43, 59, 150
MboII GAAGA 3 cut(s) 83, 158, 265
MluCI AATT 2 cut(s) 129, 196
Mph1103I ATGCAT 1 cut(s) 304
MseI TTAA 4 cut(s) 78, 191, 200, 231
NlaIII CATG 1 cut(s) 177
NmuCI GTSAC 2 cut(s) 43, 59
NsiI ATGCAT 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PfeI GAWTC 1 cut(s) 34
PkrI GCNGC 3 cut(s) 52, 187, 283
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 4 cut(s) 78, 191, 200, 231
SatI GCNGC 3 cut(s) 51, 186, 282
SetI ASST 6 cut(s) 14, 31, 78, 159, 209, 231
SfaNI GCATC 1 cut(s) 84
SgeI CNNG 9 cut(s) 50, 160, 167, 186, 219, 223, 276, 284, 306
Sse9I AATT 2 cut(s) 129, 196
SsiI CCGC 2 cut(s) 261, 330
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 159
TasI AATT 2 cut(s) 129, 196
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 4 cut(s) 78, 191, 200, 231
Tru9I TTAA 4 cut(s) 78, 191, 200, 231
TseFI GTSAC 2 cut(s) 43, 59
TseI GCWGC 3 cut(s) 50, 185, 281
Tsp45I GTSAC 2 cut(s) 43, 59
XmiI GTMKAC 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.