Rh7AG192700

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009725
Physical Location & Seq
Forward (+)
17219905 .. 17223375
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG192700.1

Sequence Viewer

Length: 690 bp
ATGCCACAAGGTTTCCTAACTTGTGCTACAACTCTCTCAAAATCTACTCAAGAGAAATCCAAACCAAACCCTAAAATACTAGCTCATACTGCTCTCAGTGTAGCTTTTGAATTTACCAAACAAACTCATGCAACAGTGAAAAAGTTTTCAACATTTCAAGGTTTAGGTCCACTAGTGAGACCAACAATCTGTGATTACGGGAAGCAACTTGATATCACTGTGGAGGAGCTCCAAAATTCTAAAGATCAACTATGTAAAATAGTTCAAGTCTCTAATTCTGGATTTCAAATAAGCAATTTTCAAACATGGGTTAGTGGTGCACTAACAAATGAGAATACATGCATGGATGGCATTGGCGAGATCACTCTAAAGGGGAAAGTGGAGATTGTTTCTTATAGGCACATAGCTAAAGTTGCACAATTAACGATCAATGCCTTGACACTCATCAACAGTTATGTTAACCGAAGAGCTCGACAGGATCTGCACCGCAACCTACCGCCGACACCGACGAGCTCCTCAGTCGACGTTGACGAGGTCCGCTCACCAGAACCTACCATTGAGGCCGATGAGCTCCTCCTTCACAAAATAAACTTGCTCTCATGTCCTTTGGAGAAAGCAGCTAAATATTGTCATTTTGTTCAACGTTTGGAGAGAGTTTCTACACAGCTTGCATACAAGGTTCTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

229

Amino Acids

25.47

Weight (kDa)

8.86

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 7 - 148 2.4e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 1 cut(s) 522
AciI CCGC 3 cut(s) 487, 497, 538
AclI AACGTT 1 cut(s) 643
AclWI GGATC 1 cut(s) 486
AcsI RAATTY 2 cut(s) 110, 235
AgsI TTSAA 7 cut(s) 110, 150, 158, 266, 287, 302, 641
AhlI ACTAGT 1 cut(s) 172
AluBI AGCT 9 cut(s) 83, 104, 229, 407, 470, 513, 571, 620, 667
AluI AGCT 9 cut(s) 83, 104, 229, 407, 470, 513, 571, 620, 667
Alw21I GWGCWC 5 cut(s) 231, 322, 472, 515, 573
Alw26I GTCTC 2 cut(s) 172, 274
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 1 cut(s) 486
AlwNI CAGNNNCTG 1 cut(s) 481
AoxI GGCC 1 cut(s) 561
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 1 cut(s) 617
ApoI RAATTY 2 cut(s) 110, 235
AspS9I GGNCC 2 cut(s) 167, 535
AsuHPI GGTGA 1 cut(s) 534
AvaII GGWCC 2 cut(s) 167, 535
BaeGI GKGCMC 1 cut(s) 322
BanII GRGCYC 4 cut(s) 231, 472, 515, 573
Bbv12I GWGCWC 5 cut(s) 231, 322, 472, 515, 573
BbvI GCAGC 1 cut(s) 629
BccI CCATC 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 172, 274
BcuI ACTAGT 1 cut(s) 172
BfaI CTAG 2 cut(s) 80, 173
BisI GCNGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 619
Bme18I GGWCC 2 cut(s) 167, 535
BmgT120I GGNCC 2 cut(s) 167, 535
BplI GAGNNNNNCTC 2 cut(s) 524, 556
BpuEI CTTGAG 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 172
BseGI GGATG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 109, 531
BseRI GAGGAG 3 cut(s) 239, 505, 563
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 1 cut(s) 629
BsgI GTGCAG 1 cut(s) 467
BshFI GGCC 1 cut(s) 563
BsiHKAI GWGCWC 5 cut(s) 231, 322, 472, 515, 573
BsmAI GTCTC 2 cut(s) 172, 274
BsnI GGCC 1 cut(s) 563
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 5 cut(s) 231, 322, 472, 515, 573
Bsp143I GATC 4 cut(s) 244, 360, 426, 478
BspACI CCGC 3 cut(s) 487, 497, 538
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 2 cut(s) 108, 530
BspPI GGATC 1 cut(s) 486
BspQI GCTCTTC 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 540
BssMI GATC 4 cut(s) 244, 360, 426, 478
Bst4CI ACNGT 3 cut(s) 136, 220, 452
Bst6I CTCTTC 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 669
BstDEI CTNAG 2 cut(s) 95, 517
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 4 cut(s) 247, 363, 429, 481
BstMAI GTCTC 2 cut(s) 172, 274
BstMBI GATC 4 cut(s) 244, 360, 426, 478
BstMWI GCNNNNNNNGC 3 cut(s) 89, 348, 413
BstNSI RCATGY 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 629
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 563
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 103, 141, 216
Cac8I GCNNGC 1 cut(s) 669
CaiI CAGNNNCTG 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 167, 535
CviAII CATG 5 cut(s) 128, 306, 339, 343, 600
DdeI CTNAG 2 cut(s) 95, 517
DpnI GATC 4 cut(s) 246, 362, 428, 480
DpnII GATC 4 cut(s) 244, 360, 426, 478
Eam1104I CTCTTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 460
Ecl136II GAGCTC 4 cut(s) 229, 470, 513, 571
Eco24I GRGCYC 4 cut(s) 231, 472, 515, 573
Eco31I GGTCTC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 167, 535
Eco53kI GAGCTC 4 cut(s) 229, 470, 513, 571
EcoICRI GAGCTC 4 cut(s) 229, 470, 513, 571
EcoRV GATATC 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 344
EcoT38I GRGCYC 4 cut(s) 231, 472, 515, 573
FaeI CATG 5 cut(s) 131, 309, 342, 346, 603
FatI CATG 5 cut(s) 127, 305, 338, 342, 599
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 1 cut(s) 618
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 4 cut(s) 231, 472, 515, 573
Fsp4HI GCNGC 1 cut(s) 618
FspBI CTAG 2 cut(s) 80, 173
GluI GCNGC 1 cut(s) 618
HaeIII GGCC 1 cut(s) 563
Hin1II CATG 5 cut(s) 131, 309, 342, 346, 603
HincII GTYRAC 3 cut(s) 460, 523, 529
HindII GTYRAC 3 cut(s) 460, 523, 529
HpaI GTTAAC 1 cut(s) 460
HphI GGTGA 1 cut(s) 534
Hpy166II GTNNAC 5 cut(s) 170, 320, 460, 523, 529
Hpy188III TCNNGA 2 cut(s) 50, 279
Hpy8I GTNNAC 5 cut(s) 170, 320, 460, 523, 529
Hpy99I CGWCG 2 cut(s) 511, 527
HpyAV CCTTC 1 cut(s) 587
HpyCH4III ACNGT 3 cut(s) 136, 220, 452
HpyCH4IV ACGT 2 cut(s) 525, 643
HpyCH4V TGCA 6 cut(s) 131, 320, 342, 416, 484, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 348, 413
HpyF3I CTNAG 2 cut(s) 95, 517
HpySE526I ACGT 2 cut(s) 525, 643
Hsp92II CATG 5 cut(s) 131, 309, 342, 346, 603
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 4 cut(s) 244, 360, 426, 478
LguI GCTCTTC 1 cut(s) 460
LmnI GCTCC 4 cut(s) 226, 234, 518, 576
LpnPI CCDG 3 cut(s) 264, 461, 558
Lsp1109I GCAGC 1 cut(s) 629
MaeI CTAG 2 cut(s) 80, 173
MaeII ACGT 2 cut(s) 525, 643
MalI GATC 4 cut(s) 246, 362, 428, 480
MbiI CCGCTC 1 cut(s) 540
MboI GATC 4 cut(s) 244, 360, 426, 478
MboII GAAGA 1 cut(s) 477
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 5 cut(s) 231, 322, 472, 515, 573
MluCI AATT 5 cut(s) 110, 235, 274, 295, 419
MnlI CCTC 5 cut(s) 217, 526, 526, 553, 584
Mph1103I ATGCAT 1 cut(s) 344
MseI TTAA 3 cut(s) 422, 459, 688
MwoI GCNNNNNNNGC 3 cut(s) 89, 348, 413
NdeII GATC 4 cut(s) 244, 360, 426, 478
NlaIII CATG 5 cut(s) 131, 309, 342, 346, 603
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PflFI GACNNNGTC 1 cut(s) 533
PkrI GCNGC 1 cut(s) 619
Psp124BI GAGCTC 4 cut(s) 231, 472, 515, 573
Psp1406I AACGTT 1 cut(s) 643
PspPI GGNCC 2 cut(s) 167, 535
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 533
SacI GAGCTC 4 cut(s) 231, 472, 515, 573
SalI GTCGAC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 460
SaqAI TTAA 3 cut(s) 422, 459, 688
SatI GCNGC 1 cut(s) 618
Sau3AI GATC 4 cut(s) 244, 360, 426, 478
Sau96I GGNCC 2 cut(s) 167, 535
SduI GDGCHC 5 cut(s) 231, 322, 472, 515, 573
SinI GGWCC 2 cut(s) 167, 535
SmlI CTYRAG 1 cut(s) 48
SmoI CTYRAG 1 cut(s) 48
SpeI ACTAGT 1 cut(s) 172
Sse9I AATT 5 cut(s) 110, 235, 274, 295, 419
SsiI CCGC 3 cut(s) 487, 497, 538
SspI AATATT 1 cut(s) 626
SspMI CTAG 2 cut(s) 80, 173
SstI GAGCTC 4 cut(s) 231, 472, 515, 573
TaaI ACNGT 3 cut(s) 136, 220, 452
TaiI ACGT 2 cut(s) 528, 646
TaqI TCGA 2 cut(s) 472, 522
TasI AATT 5 cut(s) 110, 235, 274, 295, 419
Tru1I TTAA 3 cut(s) 422, 459, 688
Tru9I TTAA 3 cut(s) 422, 459, 688
TscAI CASTG 3 cut(s) 103, 141, 223
TseI GCWGC 1 cut(s) 617
TspRI CASTG 3 cut(s) 103, 141, 223
Tth111I GACNNNGTC 1 cut(s) 533
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 2 cut(s) 167, 535
XapI RAATTY 2 cut(s) 110, 235
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 2 cut(s) 80, 173
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.