Rh7DG431800

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009725
Physical Location & Seq
Forward (+)
61402190 .. 61402486
297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG431800.1

Sequence Viewer

Length: 297 bp
ATGCGTGACTGTGTGGAGGAGCTCAATGTCGCTGTGCAGATGCTCCAAAAGTCTAAAGTTGAGTTGGGTATGGTAATTGAAGGCTCTACTTCCGGGTTACACGTTAGTAATGTTCAAACATGGTTTAGCGCTGCATTAACCAATTTAAACACCTGCAAGGATGGATTCGTCGACCATAATCTAAATGGAACGGTAGAGAAAATTACCACCAGAAAAATTGTAAAAGCAGCACATTTGACGAGCAATGCCTTGGCACTCATCAACTTGTGTATCTCCGCTAAAGTTACCTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

98

Amino Acids

10.6

Weight (kDa)

7.76

Isoelectric Point (pI)

9.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 1 - 86 4.3e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 276
AfeI AGCGCT 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 2 cut(s) 80, 116
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Alw21I GWGCWC 1 cut(s) 24
Aor51HI AGCGCT 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 131, 227
AspLEI GCGC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 94
BaeI ACNNNNGTAYC 2 cut(s) 253, 286
BanII GRGCYC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 118, 239
BccI CCATC 1 cut(s) 155
BcnI CCSGG 1 cut(s) 94
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 2 cut(s) 132, 228
BlsI GCNGC 2 cut(s) 133, 229
Bme1390I CCNGG 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 94
BmsI GCATC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 118, 239
BsgI GTGCAG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 24
BspACI CCGC 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 11, 193
BstF5I GGATG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 118, 239
BtsCI GGATG 1 cut(s) 166
BveI ACCTGC 1 cut(s) 161
CfoI GCGC 1 cut(s) 131
CviAII CATG 1 cut(s) 120
CviJI RGCY 2 cut(s) 22, 84
CviKI_1 RGCY 2 cut(s) 22, 84
DraI TTTAAA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 24
Eco47III AGCGCT 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 123
FaiI YATR 3 cut(s) 71, 121, 177
FatI CATG 1 cut(s) 119
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 132, 228
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 132, 228
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 132, 228
HaeII RGCGCY 1 cut(s) 132
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 131
Hin1II CATG 1 cut(s) 123
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 165
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 2 cut(s) 11, 193
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 3 cut(s) 37, 134, 156
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 1 cut(s) 123
HspAI GCGC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 19, 48
LpnPI CCDG 3 cut(s) 106, 166, 223
Lsp1109I GCAGC 2 cut(s) 118, 239
LweI GCATC 1 cut(s) 30
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 3 cut(s) 5, 96, 283
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 4 cut(s) 75, 142, 201, 216
MnlI CCTC 1 cut(s) 10
MseI TTAA 2 cut(s) 137, 146
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 94
NciI CCSGG 1 cut(s) 94
NlaIII CATG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 5
PaqCI CACCTGC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 165
PkrI GCNGC 2 cut(s) 133, 229
Psp124BI GAGCTC 1 cut(s) 24
SacI GAGCTC 1 cut(s) 24
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 137, 146
SatI GCNGC 2 cut(s) 132, 228
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 1 cut(s) 24
SetI ASST 4 cut(s) 24, 105, 155, 290
SfaNI GCATC 1 cut(s) 30
Sse9I AATT 4 cut(s) 75, 142, 201, 216
SsiI CCGC 1 cut(s) 276
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 11, 193
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 171
TasI AATT 4 cut(s) 75, 142, 201, 216
TfiI GAWTC 1 cut(s) 165
Tru1I TTAA 2 cut(s) 137, 146
Tru9I TTAA 2 cut(s) 137, 146
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 2 cut(s) 131, 227
Tsp45I GTSAC 1 cut(s) 5
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.