MD04G1170800.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002638
Physical Location & Seq
Reverse (-)
26220676 .. 26230376
9701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1170800.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGATGCATCATAATCCCTTGGGAAGCCTCATGGTCAGTCGCAACGCATTGATGTGGATGGCGGGAACGCTCATGGTCGCCACCTTCCTCGCCGCCTCCAACGTCCATGCTTACCCTCTGACCCCTTCCAAAGACAACTACAAGCTGGCGCTCCACAAGGCTCTAATGTTTTTCAATGCGCAGCGATCCGGAAGACTACCAAGGCATAACAATGTGTCTTGGAGGAGTGACTCGTGCTTGAGAGATGGAAAATTCACCGATACTCTTAATGATCTGGTGGGTGGATATTACGATGCTGGAAATGCCATCAAGTTCAACTTCCCTGCATCCTTCGCCATGACTATGCTCAGCTGGAGTGTCATCGAGTATAGTGCTAAATACAAAGCTGCTGGTGAGCTCGCCCACGTTAAGGACATTATCAAATGGGGCACGGATTACCTTCTCAAGACCTTCAACAACTCTGCTGATTCCATTGATAGAGTCATTGACCAGGTTGGTTTGGGGAATAATGATGGAGATAGTGAAAATCCAATTACTACAGATGGTGATATTTTTTGTTGGATGCGTCCTGAAGATATTGATTACATACGTCCTGTGACCGCATGCCACGCTTGCTCCGATCTAGCTGCGGAAATGGCCGCATCTCTAGCCGCTGCATCCATTGTTTTTAAAGACCAGAAAGCCTACTCAAAAAAACTCGTCCATGGTGCCAAAACGCTCTTCAAGTTTTCAAGGAATGAGAGAGGCAGATACAGTTTCCCTGGTACAGATGCGGAACTCTTCTATAATTCTACCAGTTACTGGGATGAATTCATATGGGGTGCAGCTTGGTTGTACTACGCAACAGGCAACAACTCTTATCTTCAGCTTGCAACAACTTCTGGTCTTGCCAAACACGCTGCCTTCTCAAAGGGGCCTATTCATGGAGCGTTGAGCTGGGACAACAAACTTCTTGGTGCTCAGGTACTTCTAACCCGTTTGAGGTTATTCATAAATCCTGGGTACCCATATAGAGAAACTTTGAGAAGATTTCACAATGAGGCTAACACAATCATGTGCTCATACCTCCCATATTTCACAACCTTTAAAAGGACAAAAGGAGGCTTAATCCAACTGAATCATGGAAAGCCAAAACCTCTTCAATATGTAGTCAACGCAGCTTTCTTAGCTACTCTCTACAGTGACTATCTTGAAGCAGCAGATAATACCAAGTCAGCTGGGATGTTTTGTGGACCAAGCTTCTATTCTATTGATGTCCTACGTGAATTTGCTAAAACCCAAATTAATTACATACTGGGAAAAAATCCCAAAAAATTGAGCTATGTTGTAGGGTTTGGTAATCGTTTCCCAACACATGTCCACCACAGAGGTGCATCCATTCCGAACAACAAGATGAAGTACAGTTGCGATGGGGGTTGGAAATGGAGGGACACTTCAAAGCCAAACCCTAACACAATTGATGGTGCTATGGTTGCTGGCCCAGACAAAGATGATGGCTTCCATGATGTTCGTACTAATAACAATTACACTGAGGCTACTCTGGCAGGAAATGCAGGTTTAGTGGCAGCACTTGTTGCTTTGTCAGGAGACAAAGCTACTGGGATTGACAAAAATACCATTTTCAACGCCATTCCGCCGATGTTTCCAACTCCTCCACCACCACTGGCGTCACGAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

561

Amino Acids

62.13

Weight (kDa)

9.15

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 47 - 524 3.6e-138 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 1544
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 2 cut(s) 707, 1002
AccB7I CCANNNNNTGG 1 cut(s) 801
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 8 cut(s) 62, 93, 600, 629, 639, 651, 773, 1634
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 636
AcsI RAATTY 3 cut(s) 251, 809, 1265
AcuI CTGAAG 2 cut(s) 591, 848
AcyI GRCGYC 1 cut(s) 1667
AfaI GTAC 6 cut(s) 766, 836, 966, 1004, 1400, 1513
AfiI CCNNNNNNNGG 5 cut(s) 409, 801, 923, 981, 1089
AflIII ACRYGT 1 cut(s) 1354
AgsI TTSAA 9 cut(s) 175, 316, 454, 724, 732, 1142, 1193, 1437, 1624
AjnI CCWGG 3 cut(s) 489, 760, 997
AjuI GAANNNNNNNTTGG 4 cut(s) 704, 736, 1228, 1260
AleI CACNNNNGTG 1 cut(s) 1368
Alw21I GWGCWC 3 cut(s) 399, 961, 1061
Alw26I GTCTC 1 cut(s) 1581
AlwI GGATC 1 cut(s) 180
AlwNI CAGNNNCTG 1 cut(s) 801
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 3 cut(s) 636, 914, 1477
ApeKI GCWGC 9 cut(s) 181, 386, 626, 653, 824, 899, 1157, 1196, 1565
ApoI RAATTY 3 cut(s) 251, 809, 1265
AseI ATTAAT 1 cut(s) 1284
Asp718I GGTACC 1 cut(s) 1002
AspLEI GCGC 2 cut(s) 151, 181
AspS9I GGNCC 3 cut(s) 914, 1232, 1478
AsuHPI GGTGA 3 cut(s) 247, 404, 557
AvaII GGWCC 1 cut(s) 1232
BaeGI GKGCMC 1 cut(s) 431
BanI GGYRCC 2 cut(s) 707, 1002
BanII GRGCYC 1 cut(s) 399
BauI CACGAG 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 3 cut(s) 399, 961, 1061
BbvI GCAGC 9 cut(s) 193, 373, 613, 640, 836, 886, 1169, 1208, 1577
BccI CCATC 8 cut(s) 52, 239, 314, 506, 536, 1403, 1454, 1487
BcgI CGANNNNNNTGC 4 cut(s) 353, 387, 608, 642
BciT130I CCWGG 3 cut(s) 491, 762, 999
BcoDI GTCTC 1 cut(s) 1581
BfaI CTAG 2 cut(s) 623, 647
BfmI CTRYAG 2 cut(s) 537, 1177
BfoI RGCGCY 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 1544
BlpI GCTNAGC 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 491, 762, 999
Bme18I GGWCC 1 cut(s) 1232
BmgT120I GGNCC 3 cut(s) 914, 1232, 1478
BmiI GGNNCC 3 cut(s) 709, 915, 1004
BmrFI CCNGG 3 cut(s) 491, 762, 999
BmrI ACTGGG 3 cut(s) 811, 1304, 1608
BmsI GCATC 8 cut(s) 16, 283, 335, 552, 650, 665, 760, 1382
BmuI ACTGGG 3 cut(s) 811, 1304, 1608
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 960
Bpu1102I GCTNAGC 1 cut(s) 347
BpuEI CTTGAG 2 cut(s) 259, 428
BsaAI YACGTR 1 cut(s) 1262
BsaHI GRCGYC 1 cut(s) 1667
BsaJI CCNNGG 5 cut(s) 18, 200, 703, 760, 998
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 5 cut(s) 409, 801, 923, 981, 1089
Bse1I ACTGG 5 cut(s) 795, 806, 1299, 1603, 1668
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 3 cut(s) 491, 762, 999
BseDI CCNNGG 5 cut(s) 18, 200, 703, 760, 998
BseGI GGATG 7 cut(s) 63, 326, 567, 656, 811, 1227, 1373
BseLI CCNNNNNNNGG 5 cut(s) 409, 801, 923, 981, 1089
BseMII CTCAG 3 cut(s) 361, 974, 1521
BseNI ACTGG 5 cut(s) 795, 806, 1299, 1603, 1668
BseRI GAGGAG 2 cut(s) 238, 1641
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 9 cut(s) 193, 373, 613, 640, 836, 886, 1169, 1208, 1577
BseYI CCCAGC 2 cut(s) 936, 1217
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 3 cut(s) 638, 916, 1479
BshNI GGYRCC 2 cut(s) 707, 1002
BsiHKAI GWGCWC 3 cut(s) 399, 961, 1061
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 2 cut(s) 953, 1442
BslI CCNNNNNNNGG 5 cut(s) 409, 801, 923, 981, 1089
BsmAI GTCTC 1 cut(s) 1581
BsmFI GGGAC 2 cut(s) 953, 1442
BsnI GGCC 3 cut(s) 638, 916, 1479
Bsp1286I GDGCHC 4 cut(s) 399, 431, 961, 1061
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 3 cut(s) 185, 271, 619
Bsp1720I GCTNAGC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 703
BspACI CCGC 8 cut(s) 62, 93, 600, 629, 639, 651, 773, 1634
BspANI GGCC 3 cut(s) 638, 916, 1479
BspCNI CTCAG 3 cut(s) 360, 973, 1522
BspEI TCCGGA 1 cut(s) 188
BspLI GGNNCC 3 cut(s) 709, 915, 1004
BspMI ACCTGC 1 cut(s) 1544
BspPI GGATC 1 cut(s) 180
BspQI GCTCTTC 1 cut(s) 725
BspT107I GGYRCC 2 cut(s) 707, 1002
BsrI ACTGG 5 cut(s) 795, 806, 1299, 1603, 1668
BssECI CCNNGG 5 cut(s) 18, 200, 703, 760, 998
BssMI GATC 3 cut(s) 185, 271, 619
BssNI GRCGYC 1 cut(s) 1667
BssSI CACGAG 1 cut(s) 232
BssT1I CCWWGG 3 cut(s) 18, 200, 703
Bst2BI CACGAG 1 cut(s) 232
Bst2UI CCWGG 3 cut(s) 491, 762, 999
Bst4CI ACNGT 3 cut(s) 755, 1181, 1403
Bst6I CTCTTC 3 cut(s) 725, 785, 1143
BstACI GRCGYC 1 cut(s) 1667
BstAPI GCANNNNNTGC 2 cut(s) 1550, 1574
BstBAI YACGTR 1 cut(s) 1262
BstC8I GCNNGC 6 cut(s) 147, 399, 604, 613, 870, 1477
BstDEI CTNAG 4 cut(s) 347, 960, 1165, 1530
BstDSI CCRYGG 1 cut(s) 703
BstENI CCTNNNNNAGG 1 cut(s) 1087
BstF5I GGATG 7 cut(s) 63, 326, 567, 656, 811, 1227, 1373
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 151, 181
BstKTI GATC 3 cut(s) 188, 274, 622
BstMAI GTCTC 1 cut(s) 1581
BstMBI GATC 3 cut(s) 185, 271, 619
BstNI CCWGG 3 cut(s) 491, 762, 999
BstNSI RCATGY 2 cut(s) 606, 1358
BstSCI CCNGG 3 cut(s) 489, 760, 997
BstSFI CTRYAG 2 cut(s) 537, 1177
BstSLI GKGCMC 1 cut(s) 431
BstV1I GCAGC 9 cut(s) 193, 373, 613, 640, 836, 886, 1169, 1208, 1577
BstV2I GAAGAC 1 cut(s) 199
BsuRI GGCC 3 cut(s) 638, 916, 1479
BtgI CCRYGG 1 cut(s) 703
BtgZI GCGATG 1 cut(s) 1422
BtsCI GGATG 7 cut(s) 63, 326, 567, 656, 811, 1227, 1373
BtsIMutI CAGTG 3 cut(s) 1186, 1527, 1661
BveI ACCTGC 1 cut(s) 1544
Cac8I GCNNGC 6 cut(s) 147, 399, 604, 613, 870, 1477
CaiI CAGNNNCTG 1 cut(s) 801
CfoI GCGC 2 cut(s) 151, 181
Cfr13I GGNCC 3 cut(s) 914, 1232, 1478
CseI GACGC 2 cut(s) 554, 1656
CsiI ACCWGGT 1 cut(s) 489
Csp6I GTAC 6 cut(s) 765, 835, 965, 1003, 1399, 1512
CviQI GTAC 6 cut(s) 765, 835, 965, 1003, 1399, 1512
DdeI CTNAG 4 cut(s) 347, 960, 1165, 1530
DpnI GATC 3 cut(s) 187, 273, 621
DpnII GATC 3 cut(s) 185, 271, 619
DraI TTTAAA 2 cut(s) 670, 1087
EaeI YGGCCR 1 cut(s) 636
Eam1104I CTCTTC 3 cut(s) 725, 785, 1143
EarI CTCTTC 3 cut(s) 725, 785, 1143
EciI GGCGGA 1 cut(s) 1623
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 3 cut(s) 18, 200, 703
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 1232
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 591, 848
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 1087
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 3 cut(s) 489, 760, 997
EcoT14I CCWWGG 3 cut(s) 18, 200, 703
EcoT22I ATGCAT 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 3 cut(s) 18, 200, 703
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 2 cut(s) 953, 1442
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 815
FokI GGATG 7 cut(s) 70, 313, 574, 643, 818, 1234, 1360
FriOI GRGCYC 1 cut(s) 399
FspBI CTAG 2 cut(s) 623, 647
FspI TGCGCA 1 cut(s) 180
GlaI GCGC 2 cut(s) 150, 180
GsaI CCCAGC 2 cut(s) 940, 1221
GsuI CTGGAG 1 cut(s) 373
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 3 cut(s) 638, 916, 1479
HapII CCGG 1 cut(s) 189
HgaI GACGC 2 cut(s) 554, 1656
HhaI GCGC 2 cut(s) 151, 181
Hin1I GRCGYC 1 cut(s) 1667
Hin6I GCGC 2 cut(s) 149, 179
HinP1I GCGC 2 cut(s) 149, 179
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HindIII AAGCTT 1 cut(s) 1237
HinfI GANTC 4 cut(s) 230, 467, 480, 1117
HpaII CCGG 1 cut(s) 189
HphI GGTGA 3 cut(s) 247, 404, 557
Hpy166II GTNNAC 3 cut(s) 1153, 1232, 1360
Hpy188I TCNGA 3 cut(s) 120, 619, 1383
Hpy188III TCNNGA 6 cut(s) 189, 445, 569, 1190, 1584, 1671
Hpy8I GTNNAC 3 cut(s) 1153, 1232, 1360
HpyAV CCTTC 6 cut(s) 94, 135, 340, 449, 460, 913
HpyCH4III ACNGT 3 cut(s) 755, 1181, 1403
HpyCH4IV ACGT 4 cut(s) 102, 405, 589, 1261
HpyCH4V TGCA 7 cut(s) 7, 326, 656, 824, 872, 1373, 1553
HpyF3I CTNAG 4 cut(s) 347, 960, 1165, 1530
HpySE526I ACGT 4 cut(s) 102, 405, 589, 1261
Hsp92I GRCGYC 1 cut(s) 1667
HspAI GCGC 2 cut(s) 149, 179
Kpn2I TCCGGA 1 cut(s) 188
KpnI GGTACC 1 cut(s) 1006
Kzo9I GATC 3 cut(s) 185, 271, 619
LguI GCTCTTC 1 cut(s) 725
LmnI GCTCC 3 cut(s) 156, 620, 926
Lsp1109I GCAGC 9 cut(s) 193, 373, 613, 640, 836, 886, 1169, 1208, 1577
LweI GCATC 8 cut(s) 16, 283, 335, 552, 650, 665, 760, 1382
MabI ACCWGGT 1 cut(s) 489
MaeI CTAG 2 cut(s) 623, 647
MaeII ACGT 4 cut(s) 102, 405, 589, 1261
MaeIII GTNAC 5 cut(s) 227, 595, 797, 1181, 1668
MalI GATC 3 cut(s) 187, 273, 621
MboI GATC 3 cut(s) 185, 271, 619
MboII GAAGA 7 cut(s) 204, 584, 712, 772, 854, 1038, 1130
MfeI CAATTG 1 cut(s) 1455
MhlI GDGCHC 4 cut(s) 399, 431, 961, 1061
MlyI GAGTC 2 cut(s) 224, 489
MmeI TCCRAC 5 cut(s) 123, 539, 1135, 1397, 1670
Mph1103I ATGCAT 1 cut(s) 9
MroI TCCGGA 1 cut(s) 188
MseI TTAA 6 cut(s) 267, 408, 669, 1086, 1106, 1284
MslI CAYNNNNRTG 5 cut(s) 52, 210, 341, 1052, 1368
MspA1I CMGCKG 3 cut(s) 351, 653, 1217
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 3 cut(s) 491, 762, 999
MunI CAATTG 1 cut(s) 1455
MvaI CCWGG 3 cut(s) 491, 762, 999
NcoI CCATGG 1 cut(s) 703
NdeI CATATG 1 cut(s) 815
NdeII GATC 3 cut(s) 185, 271, 619
NlaIV GGNNCC 3 cut(s) 709, 915, 1004
NmuCI GTSAC 4 cut(s) 227, 595, 1181, 1668
NsbI TGCGCA 1 cut(s) 180
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 2 cut(s) 606, 1358
OliI CACNNNNGTG 1 cut(s) 1368
PaeI GCATGC 1 cut(s) 606
PciI ACATGT 1 cut(s) 1354
PciSI GCTCTTC 1 cut(s) 725
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PfeI GAWTC 2 cut(s) 467, 1117
PflMI CCANNNNNTGG 1 cut(s) 801
PleI GAGTC 2 cut(s) 224, 488
PpsI GAGTC 2 cut(s) 224, 488
Ppu21I YACGTR 1 cut(s) 1262
PscI ACATGT 1 cut(s) 1354
PshBI ATTAAT 1 cut(s) 1284
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 489, 760, 997
PspFI CCCAGC 2 cut(s) 936, 1217
PspGI CCWGG 3 cut(s) 489, 760, 997
PspN4I GGNNCC 3 cut(s) 709, 915, 1004
PspPI GGNCC 3 cut(s) 914, 1232, 1478
PstNI CAGNNNCTG 1 cut(s) 801
PvuII CAGCTG 2 cut(s) 351, 1217
RsaI GTAC 6 cut(s) 766, 836, 966, 1004, 1400, 1513
RsaNI GTAC 6 cut(s) 765, 835, 965, 1003, 1399, 1512
RseI CAYNNNNRTG 5 cut(s) 52, 210, 341, 1052, 1368
SacI GAGCTC 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 725
SaqAI TTAA 6 cut(s) 267, 408, 669, 1086, 1106, 1284
Sau3AI GATC 3 cut(s) 185, 271, 619
Sau96I GGNCC 3 cut(s) 914, 1232, 1478
SchI GAGTC 2 cut(s) 224, 489
ScrFI CCNGG 3 cut(s) 491, 762, 999
SduI GDGCHC 4 cut(s) 399, 431, 961, 1061
SexAI ACCWGGT 1 cut(s) 489
SfaNI GCATC 8 cut(s) 16, 283, 335, 552, 650, 665, 760, 1382
SfcI CTRYAG 2 cut(s) 537, 1177
SinI GGWCC 1 cut(s) 1232
SmiMI CAYNNNNRTG 5 cut(s) 52, 210, 341, 1052, 1368
SmlI CTYRAG 2 cut(s) 238, 443
SmoI CTYRAG 2 cut(s) 238, 443
SphI GCATGC 1 cut(s) 606
SsiI CCGC 8 cut(s) 62, 93, 600, 629, 639, 651, 773, 1634
SspMI CTAG 2 cut(s) 623, 647
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 489, 760, 997
StyI CCWWGG 3 cut(s) 18, 200, 703
TaaI ACNGT 3 cut(s) 755, 1181, 1403
TaiI ACGT 4 cut(s) 105, 408, 592, 1264
TaqI TCGA 1 cut(s) 363
TatI WGTACW 2 cut(s) 834, 1398
TauI GCSGC 3 cut(s) 95, 641, 653
TfiI GAWTC 2 cut(s) 467, 1117
Tru1I TTAA 6 cut(s) 267, 408, 669, 1086, 1106, 1284
Tru9I TTAA 6 cut(s) 267, 408, 669, 1086, 1106, 1284
TscAI CASTG 3 cut(s) 1186, 1534, 1668
TseFI GTSAC 4 cut(s) 227, 595, 1181, 1668
TseI GCWGC 9 cut(s) 181, 386, 626, 653, 824, 899, 1157, 1196, 1565
Tsp45I GTSAC 4 cut(s) 227, 595, 1181, 1668
TspDTI ATGAA 5 cut(s) 802, 822, 911, 979, 1409
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 3 cut(s) 1186, 1534, 1668
Van91I CCANNNNNTGG 1 cut(s) 801
VpaK11BI GGWCC 1 cut(s) 1232
VspI ATTAAT 1 cut(s) 1284
XagI CCTNNNNNAGG 1 cut(s) 1087
XapI RAATTY 3 cut(s) 251, 809, 1265
XceI RCATGY 2 cut(s) 606, 1358
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XspI CTAG 2 cut(s) 623, 647
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.