Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002638

Physical Location & Seq

Genomic Coordinates Forward (+)

31583528 .. 31586917

Gene Structure 3390 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1250900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1869 bp

ATGAGCATGTACGGCAGGGACCCCTGGGGGGGGCCGCTGGAGATAAACACGGCTGACTCTGCCACCGACGACGACCGCAGCCGGAACCTGCAGGATCTGGACAGGGCGGCGCTGTCGTCGCGGCCGCTGGACGAGACTCAGCAGAGCTGGCTGCTGGGGCCCTCCGGCGAGCAGAAGAAGAAGAAGTATGTGGATCTGGGATGCATCATCGTCAGCCGTAAGATCTTTGTGTGGACGGTCGGGACGCTGCTGGTCTCCGCCTTCTTGGCGGGCTTCATCACGCTCATCGTCAAGACTGTGCCGCGTCACCGCCACGCCCGCCCTCCCCCCGACAACTACACTTTGGCGCTTCGCAAGTCCCTAATGTTCTTCAACGCTCAACGATCCGGAAAACTCCCAAAGCATAACAATGTGTCGTGGAGGGGTAATTCGTGCTTAAAGGATGGCAATGATCCCTCCACCACTTTCAAAGATCTTGCCGGTGGCTTTTATGACGCCGGAGATGCTATCAAGTTCAATTTTCCCGCCTCCTTCGCCATGACCATGCTCAGCTGGAGCGTCATCGAATACAGTGCTAAGTATGAAGCTTCCGGGGAGCTTTCCCATGTTAAAGAAATCATTAAATGGGGATCCGATTATTTTTTGAAGACCTTCAACCATTCTGCCGATTCCATTGACAAACTTGTTGCCCAGGTTGGGGTAGGATCTACTGCCGGAGGAAGTACTACTCCTAATGATCATTATTGCTGGATGCGTCCTGAGGATATTGATTACCAGCGTCCTGTGTCTGAGTGTCACAGTTGCTCTGATCTAGCTGCGGAAATGGCCGCGGCGCTAGCTGCTTCGTCCATAGTTTTCAAAGATAACAAGGCATACTCTCAGAAACTTGTCCATGGTGCAAAAACACTCTTCAGATTTTCAAGGGAGCAGAGAGGCAGATATACTGCCGGTGGTAGTACAGATGCGGCAACCTTCTACAATTCCACCAGCTATTGGGATGAGTTCATATGGGGTGGAGCTTGGATGTATTATGCTACTGGAAATTCCTCTTATCTTCAGCTTGCAACAACTCCAGGTCTTGCTAAACATGCCGGTGCTTTCTGGGGAGGACCTGATTATGGAGTATTGAGCTGGGACAACAAGCTTGCTGGAGCTCAGGTGCTTCTGAGCCGTTTGAGGTTATTCTTGAGCCCTGGGTATCCGTATGAAGAAATTTTGAGGACATTTCACAACCAGACTAGCATAATCATGTGCTCATACTTGCCTGTTTTTACAACCTTTAACAGAACCAAAGGAGGCTTGATTCAGTTGAATCATGGAAGGCCACAACCTCTTCAATATGTAGTCAATGCAGCCTTCTTAGCTACTTTGTATAGTGATTATCTTGATGCTGCTGATACACCTGGGTGGTATTGTGGACCCAACTTCTACTCTACTGACGTTTTGCGAGAATTTGCCAAAACTCAGATTGATTACATCCTTGGCAAAAATCCTCGAAAAATGAGTTATGTCGTGGGCTTTGGGAATCATTACCCAAAACATGTCCACCACAGAGGCGCATCTATTCCAAAGAACAAAATCAAATACAGTTGTAAGGGGGGATGGAAATGGAGAGACACTTCAAAGGCAAATCCAAACACGATTGATGGGGCCATGGTTGCTGGCCCTGACAAACATGATGGTTTCCGTGATGTTCGTTCTAATTACAACTACACAGAGCCGACTCTCGCAGGAAATGCAGGTTTGGTAGCAGCACTCGTTGCTTTGTCTGGAGAAAAATCTGTTGGAATTGACAAAAATACGATTTTCTCTGCGGTTCCTCCAATGTTCCCCACTCCACCACCACCTCCGGCACCCTGGAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007275 GO:0008150 GO:0008152 GO:0008810 GO:0009058 GO:0009059 GO:0009250 GO:0009504 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0030243 GO:0030244 GO:0030865 GO:0031122 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0033692 GO:0034637 GO:0034645 GO:0040007 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043622 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048367 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0060560 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0097708 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_9 Mlo

Protein Analysis

623

Amino Acids

68.91

Weight (kDa)

9.04

Isoelectric Point (pI)

37.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_9	PF00759	113 - 586	6.4e-143	Glycosyl hydrolase family 9

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002638)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24260 AT4G24260 AT4G24260 AT5G49720
fragaria_vesca	FvH4_5g27130 FvH4_5g27130 FvH4_5g27130 FvH4_5g27130
malus_domestica	MD04G1170800.v1.1 MD08G1250900.v1.1 MD15G1445800.v1.1
prunus_persica	Prupe.1G587900_v2.0.a1 Prupe.3G082700_v2.0.a1
pyrus_communis	pycom04g15140 pycom08g21760 pycom15g39260 pycom15g39270
rosa_chinensis	RchiOBHm_Chr7g0218131
rosa_laevigata	RLG00000002397 RLG00000003204 RLG00000029460
rosa_multiflora	Rmu_sc0000054.1_g000022 Rmu_sc0003627.1_g000012 Rmu_sc0031732.1_g000002
rosa_roxburghii	Rroxscaffold_3G00233940 Rroxscaffold_3G00241280
rosa_rugosa	Rorug01G0072800 Rorug02G0384500 Rorug04G0442400 Rorug07G0174400
rosa_samantha	Rh4CG311700 Rh4DG293000 Rh6BG152200 Rh6DG136400 Rh7AG316800 Rh7BG307900 Rh7BG308000 Rh7CG333700 Rh7DG315700
rosa_wichuraiana	Rw7G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	96, 1730
AccB1I	GGYRCC	1 cut(s)	1852
AccB7I	CCANNNNNTGG	1 cut(s)	993
AccII	CGCG	3 cut(s)	121, 304, 830
AccIII	TCCGGA	1 cut(s)	386
AclWI	GGATC	7 cut(s)	102, 201, 378, 446, 624, 637, 712
AcoI	YGGCCR	2 cut(s)	122, 825
AcsI	RAATTY	3 cut(s)	1042, 1212, 1451
AcuI	CTGAAG	2 cut(s)	895, 1040
AcyI	GRCGYC	1 cut(s)	495
AfaI	GTAC	3 cut(s)	11, 724, 958
AfiI	CCNNNNNNNGG	7 cut(s)	28, 29, 30, 696, 697, 993, 1118
AflIII	ACRYGT	1 cut(s)	1540
AjnI	CCWGG	6 cut(s)	23, 690, 1072, 1192, 1402, 1856
AjuI	GAANNNNNNNTTGG	2 cut(s)	1767, 1799
AleI	CACNNNNGTG	1 cut(s)	1405
Alw21I	GWGCWC	2 cut(s)	1156, 1256
Alw26I	GTCTC	3 cut(s)	128, 259, 1608
AlwI	GGATC	7 cut(s)	102, 201, 378, 446, 624, 637, 712
AlwNI	CAGNNNCTG	1 cut(s)	97
Aor13HI	TCCGGA	1 cut(s)	386
AoxI	GGCC	7 cut(s)	32, 122, 158, 825, 1322, 1650, 1663
ApaI	GGGCCC	1 cut(s)	162
ApeKI	GCWGC	8 cut(s)	78, 151, 247, 815, 839, 1352, 1391, 1751
ApoI	RAATTY	3 cut(s)	1042, 1212, 1451
Asp700I	GAANNNNTTC	1 cut(s)	650
AspLEI	GCGC	4 cut(s)	112, 349, 835, 1559
AspS9I	GGNCC	8 cut(s)	19, 32, 158, 159, 1109, 1418, 1650, 1664
AsuC2I	CCSGG	1 cut(s)	592
AsuHPI	GGTGA	1 cut(s)	299
AsuNHI	GCTAGC	1 cut(s)	835
AvaII	GGWCC	3 cut(s)	19, 1109, 1418
AxyI	CCTNAGG	1 cut(s)	759
BaeGI	GKGCMC	1 cut(s)	162
BamHI	GGATCC	1 cut(s)	629
BanI	GGYRCC	1 cut(s)	1852
BanII	GRGCYC	3 cut(s)	162, 1156, 1193
BbsI	GAAGAC	1 cut(s)	653
Bbv12I	GWGCWC	2 cut(s)	1156, 1256
BbvI	GCAGC	8 cut(s)	90, 138, 234, 802, 826, 1364, 1378, 1763
BccI	CCATC	4 cut(s)	437, 1596, 1640, 1673
BceAI	ACGGC	4 cut(s)	28, 66, 201, 1155
BcgI	CGANNNNNNTGC	2 cut(s)	554, 588
BciT130I	CCWGG	6 cut(s)	25, 692, 1074, 1194, 1404, 1858
BciVI	GTATCC	1 cut(s)	1209
BclI	TGATCA	1 cut(s)	736
BcnI	CCSGG	1 cut(s)	592
BcoDI	GTCTC	3 cut(s)	128, 259, 1608
BfaI	CTAG	3 cut(s)	812, 836, 1239
BfmI	CTRYAG	1 cut(s)	89
BfoI	RGCGCY	3 cut(s)	113, 350, 836
BfuAI	ACCTGC	2 cut(s)	96, 1730
BfuI	GTATCC	1 cut(s)	1209
BglI	GCCNNNNNGGC	1 cut(s)	266
BglII	AGATCT	2 cut(s)	222, 472
BlpI	GCTNAGC	1 cut(s)	548
BmcAI	AGTACT	1 cut(s)	724
Bme1390I	CCNGG	7 cut(s)	25, 592, 692, 1074, 1194, 1404, 1858
Bme18I	GGWCC	3 cut(s)	19, 1109, 1418
BmgT120I	GGNCC	8 cut(s)	19, 32, 158, 159, 1109, 1418, 1650, 1664
BmrFI	CCNGG	7 cut(s)	25, 592, 692, 1074, 1194, 1404, 1858
BmsI	GCATC	7 cut(s)	191, 213, 493, 741, 952, 1378, 1568
BmtI	GCTAGC	1 cut(s)	839
BpiI	GAAGAC	1 cut(s)	653
BpmI	CTGGAG	5 cut(s)	59, 574, 1056, 1170, 1791
Bpu10I	CCTNAGC	1 cut(s)	1155
Bpu1102I	GCTNAGC	1 cut(s)	548
BpuEI	CTTGAG	1 cut(s)	1207
BpuMI	CCSGG	1 cut(s)	592
BsaHI	GRCGYC	1 cut(s)	495
BsaI	GGTCTC	1 cut(s)	259
BsaWI	WCCGGW	1 cut(s)	386
BsaXI	ACNNNNNCTCC	2 cut(s)	1143, 1173
Bsc4I	CCNNNNNNNGG	7 cut(s)	28, 29, 30, 696, 697, 993, 1118
Bse118I	RCCGGY	3 cut(s)	479, 947, 1091
Bse1I	ACTGG	1 cut(s)	1042
Bse21I	CCTNAGG	1 cut(s)	759
Bse3DI	GCAATG	1 cut(s)	454
BseAI	TCCGGA	1 cut(s)	386
BseBI	CCWGG	6 cut(s)	25, 692, 1074, 1194, 1404, 1858
BseGI	GGATG	7 cut(s)	206, 448, 756, 1003, 1029, 1476, 1607
BseLI	CCNNNNNNNGG	7 cut(s)	28, 29, 30, 696, 697, 993, 1118
BseMI	GCAATG	1 cut(s)	454
BseMII	CTCAG	8 cut(s)	152, 562, 750, 780, 893, 1157, 1169, 1478
BseNI	ACTGG	1 cut(s)	1042
BseSI	GKGCMC	1 cut(s)	162
BseX3I	CGGCCG	1 cut(s)	122
BseXI	GCAGC	8 cut(s)	90, 138, 234, 802, 826, 1364, 1378, 1763
BseYI	CCCAGC	2 cut(s)	154, 1131
Bsh1236I	CGCG	3 cut(s)	121, 304, 830
Bsh1285I	CGRYCG	3 cut(s)	76, 125, 240
BshFI	GGCC	7 cut(s)	34, 124, 160, 827, 1324, 1652, 1665
BshNI	GGYRCC	1 cut(s)	1852
BsiEI	CGRYCG	3 cut(s)	76, 125, 240
BsiHKAI	GWGCWC	2 cut(s)	1156, 1256
BslFI	GGGAC	4 cut(s)	32, 256, 343, 1148
BslI	CCNNNNNNNGG	7 cut(s)	28, 29, 30, 696, 697, 993, 1118
BsmAI	GTCTC	3 cut(s)	128, 259, 1608
BsmFI	GGGAC	4 cut(s)	32, 256, 343, 1148
BsnI	GGCC	7 cut(s)	34, 124, 160, 827, 1324, 1652, 1665
Bso31I	GGTCTC	1 cut(s)	259
Bsp120I	GGGCCC	1 cut(s)	158
Bsp1286I	GDGCHC	4 cut(s)	162, 1156, 1193, 1256
Bsp13I	TCCGGA	1 cut(s)	386
Bsp1720I	GCTNAGC	1 cut(s)	548
Bsp19I	CCATGG	2 cut(s)	892, 1653
BspANI	GGCC	7 cut(s)	34, 124, 160, 827, 1324, 1652, 1665
BspCNI	CTCAG	8 cut(s)	151, 561, 751, 781, 892, 1158, 1168, 1477
BspEI	TCCGGA	1 cut(s)	386
BspFNI	CGCG	3 cut(s)	121, 304, 830
BspMAI	CTGCAG	1 cut(s)	93
BspMI	ACCTGC	2 cut(s)	96, 1730
BspOI	GCTAGC	1 cut(s)	839
BspPI	GGATC	7 cut(s)	102, 201, 378, 446, 624, 637, 712
BspT107I	GGYRCC	1 cut(s)	1852
BspTNI	GGTCTC	1 cut(s)	259
BsrDI	GCAATG	1 cut(s)	454
BsrFI	RCCGGY	3 cut(s)	479, 947, 1091
BsrI	ACTGG	1 cut(s)	1042
BssAI	RCCGGY	3 cut(s)	479, 947, 1091
BssNI	GRCGYC	1 cut(s)	495
BssT1I	CCWWGG	3 cut(s)	892, 1480, 1653
Bst2UI	CCWGG	6 cut(s)	25, 692, 1074, 1194, 1404, 1858
Bst4CI	ACNGT	5 cut(s)	238, 298, 572, 800, 1589
Bst6I	CTCTTC	2 cut(s)	914, 1338
BstACI	GRCGYC	1 cut(s)	495
BstAPI	GCANNNNNTGC	2 cut(s)	1736, 1760
BstC8I	GCNNGC	8 cut(s)	149, 170, 271, 319, 837, 1062, 1146, 1663
BstDSI	CCRYGG	3 cut(s)	828, 892, 1653
BstF5I	GGATG	7 cut(s)	206, 448, 756, 1003, 1029, 1476, 1607
BstFNI	CGCG	3 cut(s)	121, 304, 830
BstH2I	RGCGCY	3 cut(s)	113, 350, 836
BstHHI	GCGC	4 cut(s)	112, 349, 835, 1559
BstMAI	GTCTC	3 cut(s)	128, 259, 1608
BstMCI	CGRYCG	3 cut(s)	76, 125, 240
BstNI	CCWGG	6 cut(s)	25, 692, 1074, 1194, 1404, 1858
BstNSI	RCATGY	3 cut(s)	10, 1091, 1544
BstSCI	CCNGG	7 cut(s)	23, 590, 690, 1072, 1192, 1402, 1856
BstSFI	CTRYAG	1 cut(s)	89
BstSLI	GKGCMC	1 cut(s)	162
BstUI	CGCG	3 cut(s)	121, 304, 830
BstV1I	GCAGC	8 cut(s)	90, 138, 234, 802, 826, 1364, 1378, 1763
BstV2I	GAAGAC	1 cut(s)	653
BstX2I	RGATCY	6 cut(s)	94, 193, 222, 472, 629, 704
BstYI	RGATCY	6 cut(s)	94, 193, 222, 472, 629, 704
BstZI	CGGCCG	1 cut(s)	122
Bsu36I	CCTNAGG	1 cut(s)	759
BsuI	GTATCC	1 cut(s)	1209
BsuRI	GGCC	7 cut(s)	34, 124, 160, 827, 1324, 1652, 1665
BtgI	CCRYGG	3 cut(s)	828, 892, 1653
BtsCI	GGATG	7 cut(s)	206, 448, 756, 1003, 1029, 1476, 1607
BtsIMutI	CAGTG	1 cut(s)	577
BveI	ACCTGC	2 cut(s)	96, 1730
Cac8I	GCNNGC	8 cut(s)	149, 170, 271, 319, 837, 1062, 1146, 1663
CaiI	CAGNNNCTG	1 cut(s)	97
CciNI	GCGGCCGC	1 cut(s)	122
CfoI	GCGC	4 cut(s)	112, 349, 835, 1559
Cfr10I	RCCGGY	3 cut(s)	479, 947, 1091
Cfr13I	GGNCC	8 cut(s)	19, 32, 158, 159, 1109, 1418, 1650, 1664
Cfr42I	CCGCGG	1 cut(s)	831
CseI	GACGC	6 cut(s)	253, 293, 503, 547, 743, 767
Csp6I	GTAC	3 cut(s)	10, 723, 957
CviQI	GTAC	3 cut(s)	10, 723, 957
EaeI	YGGCCR	2 cut(s)	122, 825
EagI	CGGCCG	1 cut(s)	122
Eam1104I	CTCTTC	2 cut(s)	914, 1338
EarI	CTCTTC	2 cut(s)	914, 1338
EciI	GGCGGA	1 cut(s)	247
Ecl136II	GAGCTC	1 cut(s)	1154
EclXI	CGGCCG	1 cut(s)	122
Eco130I	CCWWGG	3 cut(s)	892, 1480, 1653
Eco24I	GRGCYC	3 cut(s)	162, 1156, 1193
Eco31I	GGTCTC	1 cut(s)	259
Eco47I	GGWCC	3 cut(s)	19, 1109, 1418
Eco52I	CGGCCG	1 cut(s)	122
Eco53kI	GAGCTC	1 cut(s)	1154
Eco57I	CTGAAG	2 cut(s)	895, 1040
Eco81I	CCTNAGG	1 cut(s)	759
EcoICRI	GAGCTC	1 cut(s)	1154
EcoO109I	RGGNCCY	4 cut(s)	19, 158, 159, 1109
EcoRII	CCWGG	6 cut(s)	23, 690, 1072, 1192, 1402, 1856
EcoT14I	CCWWGG	3 cut(s)	892, 1480, 1653
EcoT22I	ATGCAT	1 cut(s)	206
EcoT38I	GRGCYC	3 cut(s)	162, 1156, 1193
ErhI	CCWWGG	3 cut(s)	892, 1480, 1653
FaqI	GGGAC	4 cut(s)	32, 256, 343, 1148
FauI	CCCGC	3 cut(s)	262, 326, 532
FauNDI	CATATG	1 cut(s)	1007
FbaI	TGATCA	1 cut(s)	736
FokI	GGATG	7 cut(s)	213, 455, 763, 1010, 1036, 1463, 1614
FriOI	GRGCYC	3 cut(s)	162, 1156, 1193
FspBI	CTAG	3 cut(s)	812, 836, 1239
GlaI	GCGC	4 cut(s)	111, 348, 834, 1558
GsaI	CCCAGC	2 cut(s)	158, 1135
GsuI	CTGGAG	5 cut(s)	59, 574, 1056, 1170, 1791
HaeII	RGCGCY	3 cut(s)	113, 350, 836
HaeIII	GGCC	7 cut(s)	34, 124, 160, 827, 1324, 1652, 1665
HgaI	GACGC	6 cut(s)	253, 293, 503, 547, 743, 767
HhaI	GCGC	4 cut(s)	112, 349, 835, 1559
Hin1I	GRCGYC	1 cut(s)	495
Hin6I	GCGC	4 cut(s)	110, 347, 833, 1557
HinP1I	GCGC	4 cut(s)	110, 347, 833, 1557
HindIII	AAGCTT	2 cut(s)	585, 1142
HinfI	GANTC	7 cut(s)	56, 136, 668, 1303, 1312, 1525, 1723
HphI	GGTGA	1 cut(s)	299
Hpy166II	GTNNAC	3 cut(s)	234, 1418, 1546
Hpy188I	TCNGA	7 cut(s)	634, 790, 808, 882, 914, 1167, 1467
Hpy188III	TCNNGA	8 cut(s)	98, 241, 292, 387, 758, 1186, 1385, 1770
Hpy8I	GTNNAC	3 cut(s)	234, 1418, 1546
Hpy99I	CGWCG	3 cut(s)	71, 74, 121
HpyAV	CCTTC	6 cut(s)	271, 541, 661, 982, 1314, 1366
HpyCH4III	ACNGT	5 cut(s)	238, 298, 572, 800, 1589
HpyCH4IV	ACGT	1 cut(s)	1440
HpyCH4V	TGCA	6 cut(s)	91, 204, 899, 1064, 1352, 1739
HpySE526I	ACGT	1 cut(s)	1440
Hsp92I	GRCGYC	1 cut(s)	495
HspAI	GCGC	4 cut(s)	110, 347, 833, 1557
KflI	GGGWCCC	1 cut(s)	19
Kpn2I	TCCGGA	1 cut(s)	386
Ksp22I	TGATCA	1 cut(s)	736
KspI	CCGCGG	1 cut(s)	831
LmnI	GCTCC	5 cut(s)	555, 595, 925, 1016, 1151
Lsp1109I	GCAGC	8 cut(s)	90, 138, 234, 802, 826, 1364, 1378, 1763
LweI	GCATC	7 cut(s)	191, 213, 493, 741, 952, 1378, 1568
MaeI	CTAG	3 cut(s)	812, 836, 1239
MaeII	ACGT	1 cut(s)	1440
MaeIII	GTNAC	2 cut(s)	305, 794
MboII	GAAGA	9 cut(s)	187, 190, 193, 361, 658, 901, 1046, 1220, 1325
MflI	RGATCY	6 cut(s)	94, 193, 222, 472, 629, 704
MhlI	GDGCHC	4 cut(s)	162, 1156, 1193, 1256
MluCI	AATT	8 cut(s)	427, 517, 979, 1042, 1212, 1451, 1702, 1788
MlyI	GAGTC	3 cut(s)	50, 130, 1717
MmeI	TCCRAC	1 cut(s)	1765
Mph1103I	ATGCAT	1 cut(s)	206
MroI	TCCGGA	1 cut(s)	386
MroXI	GAANNNNTTC	1 cut(s)	650
MseI	TTAA	4 cut(s)	437, 609, 621, 1281
MslI	CAYNNNNRTG	5 cut(s)	408, 542, 1092, 1247, 1405
MspA1I	CMGCKG	4 cut(s)	37, 127, 552, 830
MspR9I	CCNGG	7 cut(s)	25, 592, 692, 1074, 1194, 1404, 1858
MvaI	CCWGG	6 cut(s)	25, 692, 1074, 1194, 1404, 1858
MvnI	CGCG	3 cut(s)	121, 304, 830
NciI	CCSGG	1 cut(s)	592
NcoI	CCATGG	2 cut(s)	892, 1653
NdeI	CATATG	1 cut(s)	1007
NheI	GCTAGC	1 cut(s)	835
NmuCI	GTSAC	2 cut(s)	305, 794
NotI	GCGGCCGC	1 cut(s)	122
NsiI	ATGCAT	1 cut(s)	206
NspI	RCATGY	3 cut(s)	10, 1091, 1544
OliI	CACNNNNGTG	1 cut(s)	1405
PasI	CCCWGGG	2 cut(s)	24, 1193
PciI	ACATGT	1 cut(s)	1540
PdmI	GAANNNNTTC	1 cut(s)	650
PfeI	GAWTC	4 cut(s)	668, 1303, 1312, 1525
PflMI	CCANNNNNTGG	1 cut(s)	993
PleI	GAGTC	3 cut(s)	50, 130, 1717
PpsI	GAGTC	3 cut(s)	50, 130, 1717
PpuMI	RGGWCCY	2 cut(s)	19, 1109
PscI	ACATGT	1 cut(s)	1540
Psp124BI	GAGCTC	1 cut(s)	1156
Psp5II	RGGWCCY	2 cut(s)	19, 1109
Psp6I	CCWGG	6 cut(s)	23, 690, 1072, 1192, 1402, 1856
PspFI	CCCAGC	2 cut(s)	154, 1131
PspGI	CCWGG	6 cut(s)	23, 690, 1072, 1192, 1402, 1856
PspOMI	GGGCCC	1 cut(s)	158
PspPI	GGNCC	8 cut(s)	19, 32, 158, 159, 1109, 1418, 1650, 1664
PspPPI	RGGWCCY	2 cut(s)	19, 1109
PstI	CTGCAG	1 cut(s)	93
PstNI	CAGNNNCTG	1 cut(s)	97
PsuI	RGATCY	6 cut(s)	94, 193, 222, 472, 629, 704
PvuII	CAGCTG	1 cut(s)	552
RsaI	GTAC	3 cut(s)	11, 724, 958
RsaNI	GTAC	3 cut(s)	10, 723, 957
RseI	CAYNNNNRTG	5 cut(s)	408, 542, 1092, 1247, 1405
SacI	GAGCTC	1 cut(s)	1156
SacII	CCGCGG	1 cut(s)	831
SaqAI	TTAA	4 cut(s)	437, 609, 621, 1281
Sau96I	GGNCC	8 cut(s)	19, 32, 158, 159, 1109, 1418, 1650, 1664
SbfI	CCTGCAGG	1 cut(s)	93
ScaI	AGTACT	1 cut(s)	724
SchI	GAGTC	3 cut(s)	50, 130, 1717
ScrFI	CCNGG	7 cut(s)	25, 592, 692, 1074, 1194, 1404, 1858
SdaI	CCTGCAGG	1 cut(s)	93
SduI	GDGCHC	4 cut(s)	162, 1156, 1193, 1256
SfaNI	GCATC	7 cut(s)	191, 213, 493, 741, 952, 1378, 1568
SfcI	CTRYAG	1 cut(s)	89
Sfr303I	CCGCGG	1 cut(s)	831
SgrBI	CCGCGG	1 cut(s)	831
SinI	GGWCC	3 cut(s)	19, 1109, 1418
SmiMI	CAYNNNNRTG	5 cut(s)	408, 542, 1092, 1247, 1405
SmlI	CTYRAG	1 cut(s)	1186
SmoI	CTYRAG	1 cut(s)	1186
Sse8387I	CCTGCAGG	1 cut(s)	93
Sse9I	AATT	8 cut(s)	427, 517, 979, 1042, 1212, 1451, 1702, 1788
SspMI	CTAG	3 cut(s)	812, 836, 1239
SstI	GAGCTC	1 cut(s)	1156
StyD4I	CCNGG	7 cut(s)	23, 590, 690, 1072, 1192, 1402, 1856
StyI	CCWWGG	3 cut(s)	892, 1480, 1653
TaaI	ACNGT	5 cut(s)	238, 298, 572, 800, 1589
TaiI	ACGT	1 cut(s)	1443
TaqI	TCGA	2 cut(s)	564, 1495
TasI	AATT	8 cut(s)	427, 517, 979, 1042, 1212, 1451, 1702, 1788
TatI	WGTACW	2 cut(s)	722, 956
TauI	GCSGC	8 cut(s)	37, 110, 124, 127, 304, 830, 833, 968
TfiI	GAWTC	4 cut(s)	668, 1303, 1312, 1525
Tru1I	TTAA	4 cut(s)	437, 609, 621, 1281
Tru9I	TTAA	4 cut(s)	437, 609, 621, 1281
TscAI	CASTG	1 cut(s)	577
TseFI	GTSAC	2 cut(s)	305, 794
TseI	GCWGC	8 cut(s)	78, 151, 247, 815, 839, 1352, 1391, 1751
Tsp45I	GTSAC	2 cut(s)	305, 794
TspDTI	ATGAA	4 cut(s)	265, 597, 994, 1221
TspGWI	ACGGA	2 cut(s)	1191, 1676
TspRI	CASTG	1 cut(s)	577
Van91I	CCANNNNNTGG	1 cut(s)	993
VpaK11BI	GGWCC	3 cut(s)	19, 1109, 1418
XapI	RAATTY	3 cut(s)	1042, 1212, 1451
XceI	RCATGY	3 cut(s)	10, 1091, 1544
XmnI	GAANNNNTTC	1 cut(s)	650
XspI	CTAG	3 cut(s)	812, 836, 1239
ZrmI	AGTACT	1 cut(s)	724
Zsp2I	ATGCAT	1 cut(s)	206

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.