MD04G1195000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009038
Physical Location & Seq
Forward (+)
28393357 .. 28394500
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1195000.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGCTTGTATTATATTCCCAAAACACAAGTTCTTCTGCCTTACTCTTCTTCTCATTGCAGCTTGTTGCTCCTACTCAGGATACGCAGAGGACAACCCGGCGCAGACGTTTGTGACAGCCTTGGCATGTTTCACCAACAAGTTTATTTATGCTGGTTGTAATGAAGCATACAGATTGAATGAGAGTGGAGAATTTAACGTGCCTCCTGAAGCAACTAATTCGTTCTGCCATGGACCATGTTTTGAAGAGACACAACAAGTTCTGAACTGCGTTGATAGAATGTTTTCAAACTTCGTTTTCGGCAATAGAGCAACACTGCCTGAGCTGAGAAGTGCACTTCATGCTGGCTGCATCTACACAAATCAAATAGGGAGGTTCAATGGTTTTGGGCCTTTTGGTCAGTACCAAGGTGAAACAAGCAGTGCACAGAAGCTACCAAAGTTCGTCAGTTTTTTCACGTTCTTAATTGTTACTGGGTTCTGTCTCTTCATCCTACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.54

Weight (kDa)

6.39

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 33 - 127 2e-35 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 1 cut(s) 404
AgsI TTSAA 4 cut(s) 178, 245, 288, 379
AluBI AGCT 3 cut(s) 62, 325, 433
AluI AGCT 3 cut(s) 62, 325, 433
Alw21I GWGCWC 2 cut(s) 337, 427
Alw26I GTCTC 2 cut(s) 242, 488
Alw44I GTGCAC 2 cut(s) 333, 423
AoxI GGCC 1 cut(s) 389
ApaLI GTGCAC 2 cut(s) 333, 423
ApeKI GCWGC 2 cut(s) 59, 348
ApoI RAATTY 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 233, 389
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 124, 422
AvaII GGWCC 1 cut(s) 233
BaeGI GKGCMC 2 cut(s) 337, 427
Bbv12I GWGCWC 2 cut(s) 337, 427
BbvI GCAGC 2 cut(s) 71, 335
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciVI GTATCC 1 cut(s) 74
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 242, 488
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 2 cut(s) 60, 349
BlsI GCNGC 2 cut(s) 61, 350
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 233, 389
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 483
Bpu10I CCTNAGC 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 120, 229, 406
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 54
BseDI CCNNGG 3 cut(s) 120, 229, 406
BseGI GGATG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 90, 312, 317
BseNI ACTGG 1 cut(s) 478
BseSI GKGCMC 2 cut(s) 337, 427
BseXI GCAGC 2 cut(s) 71, 335
BshFI GGCC 1 cut(s) 391
BsiHKAI GWGCWC 2 cut(s) 337, 427
BsiSI CCGG 1 cut(s) 98
BsmAI GTCTC 2 cut(s) 242, 488
BsnI GGCC 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 337, 427
Bsp19I CCATGG 1 cut(s) 229
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 3 cut(s) 89, 313, 318
BsrDI GCAATG 1 cut(s) 54
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 3 cut(s) 120, 229, 406
BssT1I CCWWGG 3 cut(s) 120, 229, 406
Bst6I CTCTTC 3 cut(s) 51, 240, 491
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 3 cut(s) 76, 321, 326
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 489
BstHHI GCGC 1 cut(s) 103
BstMAI GTCTC 2 cut(s) 242, 488
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 96
BstSLI GKGCMC 2 cut(s) 337, 427
BstV1I GCAGC 2 cut(s) 71, 335
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 1 cut(s) 391
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 489
BtsI GCAGTG 2 cut(s) 314, 427
BtsIMutI CAGTG 3 cut(s) 314, 427, 496
Cac8I GCNNGC 1 cut(s) 346
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 233, 389
Csp6I GTAC 1 cut(s) 403
CviAII CATG 4 cut(s) 126, 230, 237, 341
CviJI RGCY 7 cut(s) 5, 62, 119, 325, 348, 391, 433
CviKI_1 RGCY 7 cut(s) 5, 62, 119, 325, 348, 391, 433
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 76, 321, 326
Eam1104I CTCTTC 3 cut(s) 51, 240, 491
EarI CTCTTC 3 cut(s) 51, 240, 491
Eco130I CCWWGG 3 cut(s) 120, 229, 406
Eco47I GGWCC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 228
EcoT14I CCWWGG 3 cut(s) 120, 229, 406
ErhI CCWWGG 3 cut(s) 120, 229, 406
FaeI CATG 4 cut(s) 129, 233, 240, 344
FaiI YATR 7 cut(s) 14, 127, 150, 169, 231, 238, 342
FatI CATG 4 cut(s) 125, 229, 236, 340
Fnu4HI GCNGC 2 cut(s) 60, 349
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 60, 349
GlaI GCGC 1 cut(s) 102
GluI GCNGC 2 cut(s) 60, 349
HaeIII GGCC 1 cut(s) 391
HapII CCGG 1 cut(s) 98
HhaI GCGC 1 cut(s) 103
Hin1II CATG 4 cut(s) 129, 233, 240, 344
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HpaII CCGG 1 cut(s) 98
HphI GGTGA 2 cut(s) 124, 422
Hpy166II GTNNAC 2 cut(s) 335, 425
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 2 cut(s) 78, 206
Hpy8I GTNNAC 2 cut(s) 335, 425
HpyCH4IV ACGT 3 cut(s) 107, 198, 458
HpyCH4V TGCA 4 cut(s) 59, 335, 351, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 3 cut(s) 76, 321, 326
HpySE526I ACGT 3 cut(s) 107, 198, 458
Hsp92II CATG 4 cut(s) 129, 233, 240, 344
HspAI GCGC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 7 cut(s) 63, 111, 138, 219, 330, 333, 459
Lsp1109I GCAGC 2 cut(s) 71, 335
LweI GCATC 1 cut(s) 360
MaeII ACGT 3 cut(s) 107, 198, 458
MaeIII GTNAC 2 cut(s) 112, 469
MboII GAAGA 5 cut(s) 25, 38, 41, 257, 478
MhlI GDGCHC 2 cut(s) 337, 427
MluCI AATT 3 cut(s) 191, 217, 465
MnlI CCTC 3 cut(s) 82, 213, 366
MroXI GAANNNNTTC 1 cut(s) 283
MseI TTAA 2 cut(s) 195, 464
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 229
NlaIII CATG 4 cut(s) 129, 233, 240, 344
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 283
PkrI GCNGC 2 cut(s) 61, 350
PspPI GGNCC 2 cut(s) 233, 389
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 195, 464
SatI GCNGC 2 cut(s) 60, 349
Sau96I GGNCC 2 cut(s) 233, 389
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 2 cut(s) 337, 427
SetI ASST 8 cut(s) 64, 110, 201, 327, 377, 412, 435, 461
SfaNI GCATC 1 cut(s) 360
SinI GGWCC 1 cut(s) 233
Sse9I AATT 3 cut(s) 191, 217, 465
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 3 cut(s) 120, 229, 406
TaiI ACGT 3 cut(s) 110, 201, 461
TasI AATT 3 cut(s) 191, 217, 465
Tru1I TTAA 2 cut(s) 195, 464
Tru9I TTAA 2 cut(s) 195, 464
TscAI CASTG 2 cut(s) 321, 427
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 2 cut(s) 59, 348
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 3 cut(s) 177, 329, 478
TspRI CASTG 2 cut(s) 321, 427
VneI GTGCAC 2 cut(s) 333, 423
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.