Rorug03G0016800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009038
Physical Location & Seq
Forward (+)
1323024 .. 1323407
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0016800.1

Sequence Viewer

Length: 384 bp
ATGGGAGGGGCACCAACTGTGCTGAACATGATTGTTAATTCACCAAAGAGTGACCGAAGGCCACTTCCTCACAAGGTGGAAGTAATGACAGGTGGCTCCCCACCCCCACCTCAGATCCTGTTCAATATGGAAGAGATGGCTTTTGGGGTGATTCACTTGTATGGTCTCACACAGACTTTCGGTCCAGGGACCTATTGCTCATGGAAACCTGAGTGGGACTCTCTTTCTTCATATGAGAGATCGAAGCTTAAAGCTCGGCAAGGTGTGCAGCATGTTGCTTTGGAAGAGTTTGACATAAAAGATCCCGTCACCATGGAAAGTGTACCGCCTGATGGTAAGACAATGGGTGAAATTATGCTTGAGAGCAACAGAGGAAGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.07

Weight (kDa)

5.57

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 121 6.9e-12 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AciI CCGC 1 cut(s) 326
AclWI GGATC 2 cut(s) 109, 296
AdeI CACNNNGTG 1 cut(s) 76
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 1 cut(s) 124
AhdI GACNNNNNGTC 1 cut(s) 180
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 3 cut(s) 247, 254, 378
AluI AGCT 3 cut(s) 247, 254, 378
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 109, 296
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 1 cut(s) 268
AspS9I GGNCC 2 cut(s) 182, 189
AsuHPI GGTGA 4 cut(s) 33, 160, 301, 359
AvaII GGWCC 2 cut(s) 182, 189
BaeGI GKGCMC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 280
BccI CCATC 2 cut(s) 130, 326
BciT130I CCWGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 170
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 2 cut(s) 182, 189
BmeRI GACNNNNNGTC 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 182, 189
BmiI GGNNCC 3 cut(s) 12, 97, 190
BmrFI CCNGG 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpuEI CTTGAG 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 185, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 185, 312
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 125, 201
BseSI GKGCMC 1 cut(s) 13
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 287
BshFI GGCC 1 cut(s) 61
BshNI GGYRCC 1 cut(s) 10
BslFI GGGAC 2 cut(s) 202, 230
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 202, 230
BsnI GGCC 1 cut(s) 61
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 3 cut(s) 114, 239, 301
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 1 cut(s) 326
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 2 cut(s) 124, 202
BspLI GGNNCC 3 cut(s) 12, 97, 190
BspPI GGATC 2 cut(s) 109, 296
BspT107I GGYRCC 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 185, 312
BssMI GATC 3 cut(s) 114, 239, 301
BssT1I CCWWGG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 2 cut(s) 126, 279
BstAPI GCANNNNNTGC 1 cut(s) 265
BstDEI CTNAG 2 cut(s) 111, 210
BstDSI CCRYGG 1 cut(s) 312
BstKTI GATC 3 cut(s) 117, 242, 304
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 3 cut(s) 114, 239, 301
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 2 cut(s) 114, 301
BstYI RGATCY 2 cut(s) 114, 301
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 312
CaiI CAGNNNCTG 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 182, 189
Csp6I GTAC 1 cut(s) 323
CviAII CATG 4 cut(s) 28, 201, 272, 313
CviJI RGCY 6 cut(s) 61, 96, 140, 247, 254, 378
CviKI_1 RGCY 6 cut(s) 61, 96, 140, 247, 254, 378
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 2 cut(s) 111, 210
DpnI GATC 3 cut(s) 116, 241, 303
DpnII GATC 3 cut(s) 114, 239, 301
DraIII CACNNNGTG 1 cut(s) 76
DriI GACNNNNNGTC 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 126, 279
Eam1105I GACNNNNNGTC 1 cut(s) 180
EarI CTCTTC 2 cut(s) 126, 279
Eco130I CCWWGG 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 182, 189
EcoO109I RGGNCCY 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 4 cut(s) 31, 204, 275, 316
FaqI GGGAC 2 cut(s) 202, 230
FatI CATG 4 cut(s) 27, 200, 271, 312
FauNDI CATATG 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 1 cut(s) 61
Hin1II CATG 4 cut(s) 31, 204, 275, 316
HindIII AAGCTT 2 cut(s) 245, 376
HinfI GANTC 2 cut(s) 151, 218
HphI GGTGA 4 cut(s) 33, 160, 301, 359
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 114
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 2 cut(s) 111, 210
Hsp92II CATG 4 cut(s) 31, 204, 275, 316
Kzo9I GATC 3 cut(s) 114, 239, 301
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 6 cut(s) 75, 131, 171, 198, 222, 342
Lsp1109I GCAGC 1 cut(s) 280
MaeIII GTNAC 2 cut(s) 50, 307
MalI GATC 3 cut(s) 116, 241, 303
MboI GATC 3 cut(s) 114, 239, 301
MboII GAAGA 3 cut(s) 143, 219, 296
MflI RGATCY 2 cut(s) 114, 301
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 2 cut(s) 37, 351
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 3 cut(s) 78, 120, 365
MseI TTAA 3 cut(s) 36, 249, 382
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 265
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 232
NdeII GATC 3 cut(s) 114, 239, 301
NlaIII CATG 4 cut(s) 31, 204, 275, 316
NlaIV GGNNCC 3 cut(s) 12, 97, 190
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 50, 307
NspI RCATGY 1 cut(s) 275
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 12, 97, 190
PspPI GGNCC 2 cut(s) 182, 189
PspPPI RGGWCCY 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 2 cut(s) 114, 301
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 36, 249, 382
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 114, 239, 301
Sau96I GGNCC 2 cut(s) 182, 189
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 1 cut(s) 13
SetI ASST 9 cut(s) 78, 94, 112, 194, 211, 249, 256, 265, 380
SinI GGWCC 2 cut(s) 182, 189
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 2 cut(s) 37, 351
SsiI CCGC 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 242
TaqII GACCGA 2 cut(s) 69, 170
TasI AATT 2 cut(s) 37, 351
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 36, 249, 382
Tru9I TTAA 3 cut(s) 36, 249, 382
TseFI GTSAC 2 cut(s) 50, 307
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 2 cut(s) 50, 307
TspDTI ATGAA 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 182, 189
XceI RCATGY 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.