Rorug03G0016600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009038
Physical Location & Seq
Forward (+)
1320911 .. 1321657
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0016600.1

Sequence Viewer

Length: 438 bp
ATGAATGGGCAGGGTGTTTGCTACTCTGGATTCTTGGAGCGAGCTGCAAAGGCCTACAGGGACAGGACCTCTGTAATCTATGGCTCTCTACAATACACTTGGGCTCAAACCCATCAACGCTGCCTCAAATTGGCCACTGCTCTGACCCAGTTAGGAATTTCTCACGGTGATGTTGCAACTTTGGCACCAAATGTCCCAGCTATGCTTGAGCTCTATTTTGCAGCTCCAATGGCTGGGGCAGTTCTTTATACACTTAATGCACGCCTTGATTCAGCTATGATATCTGTTCTTCTTAGTCATTCTGAAGCTAAAATCATCTTTGTCGATCACCAATTACTTGGAATTGTTGATGGAGCACTTGAATTACTTGCCAAAAAAGCTGATTCAAAAATGCCTGTTATACTGATAAGTCATCTACCAGCTTTACTTCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

15.7

Weight (kDa)

8.58

Isoelectric Point (pI)

20.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 12 - 128 1.6e-24 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 324
AfiI CCNNNNNNNGG 2 cut(s) 130, 233
AgsI TTSAA 3 cut(s) 362, 387, 431
AluBI AGCT 8 cut(s) 44, 200, 211, 224, 275, 308, 380, 422
AluI AGCT 8 cut(s) 44, 200, 211, 224, 275, 308, 380, 422
Alw21I GWGCWC 2 cut(s) 213, 358
AoxI GGCC 2 cut(s) 51, 132
ApeKI GCWGC 3 cut(s) 44, 120, 221
ApoI RAATTY 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 179, 320
AvaII GGWCC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 134
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 2 cut(s) 106, 213
Bbv12I GWGCWC 2 cut(s) 213, 358
BbvI GCAGC 3 cut(s) 31, 107, 233
BccI CCATC 2 cut(s) 120, 344
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 3 cut(s) 45, 121, 222
BlsI GCNGC 3 cut(s) 46, 122, 223
Bme18I GGWCC 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 186
BmrI ACTGGG 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 233
Bse1I ACTGG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 130, 233
BseNI ACTGG 1 cut(s) 148
BseXI GCAGC 3 cut(s) 31, 107, 233
BseYI CCCAGC 2 cut(s) 196, 233
BshFI GGCC 2 cut(s) 53, 134
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 213, 358
BslFI GGGAC 2 cut(s) 74, 179
BslI CCNNNNNNNGG 2 cut(s) 130, 233
BsmFI GGGAC 2 cut(s) 74, 179
BsnI GGCC 2 cut(s) 53, 134
Bsp1286I GDGCHC 3 cut(s) 106, 213, 358
Bsp143I GATC 1 cut(s) 325
BspANI GGCC 2 cut(s) 53, 134
BspLI GGNNCC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 184
BsrI ACTGG 1 cut(s) 148
BssMI GATC 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 42, 262
BstDEI CTNAG 1 cut(s) 293
BstKTI GATC 1 cut(s) 328
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 4 cut(s) 50, 182, 230, 377
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 31, 107, 233
BstXI CCANNNNNNTGG 1 cut(s) 338
BsuRI GGCC 2 cut(s) 53, 134
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 42, 262
Cfr13I GGNCC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 2 cut(s) 106, 213
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 66
EcoRV GATATC 1 cut(s) 282
EcoT38I GRGCYC 2 cut(s) 106, 213
FaiI YATR 5 cut(s) 81, 203, 249, 278, 401
FaqI GGGAC 2 cut(s) 74, 179
Fnu4HI GCNGC 3 cut(s) 45, 121, 222
FriOI GRGCYC 2 cut(s) 106, 213
Fsp4HI GCNGC 3 cut(s) 45, 121, 222
GluI GCNGC 3 cut(s) 45, 121, 222
GsaI CCCAGC 2 cut(s) 200, 237
HaeIII GGCC 2 cut(s) 53, 134
HinfI GANTC 3 cut(s) 30, 269, 383
HphI GGTGA 2 cut(s) 179, 320
Hpy188I TCNGA 2 cut(s) 144, 304
Hpy188III TCNNGA 1 cut(s) 27
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4V TGCA 4 cut(s) 47, 176, 221, 260
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 182, 230, 377
HpyF3I CTNAG 1 cut(s) 293
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 3 cut(s) 37, 229, 353
LpnPI CCDG 8 cut(s) 12, 43, 49, 161, 210, 219, 408, 432
Lsp1109I GCAGC 3 cut(s) 31, 107, 233
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 1 cut(s) 281
MhlI GDGCHC 3 cut(s) 106, 213, 358
MlsI TGGCCA 1 cut(s) 134
MluCI AATT 5 cut(s) 128, 156, 332, 342, 362
MluNI TGGCCA 1 cut(s) 134
MnlI CCTC 2 cut(s) 79, 134
Mox20I TGGCCA 1 cut(s) 134
MscI TGGCCA 1 cut(s) 134
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 134
MwoI GCNNNNNNNGC 4 cut(s) 50, 182, 230, 377
NdeII GATC 1 cut(s) 325
NlaIV GGNNCC 1 cut(s) 186
PceI AGGCCT 1 cut(s) 53
PfeI GAWTC 3 cut(s) 30, 269, 383
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 3 cut(s) 46, 122, 223
PpuMI RGGWCCY 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 196, 233
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 1 cut(s) 66
PspPPI RGGWCCY 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 168
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 3 cut(s) 45, 121, 222
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 1 cut(s) 66
SduI GDGCHC 3 cut(s) 106, 213, 358
SetI ASST 9 cut(s) 46, 71, 202, 213, 226, 277, 310, 382, 424
SfcI CTRYAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 128, 156, 332, 342, 362
SseBI AGGCCT 1 cut(s) 53
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 53
TaaI ACNGT 1 cut(s) 167
TaqI TCGA 1 cut(s) 324
TasI AATT 5 cut(s) 128, 156, 332, 342, 362
TfiI GAWTC 3 cut(s) 30, 269, 383
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 142
TseI GCWGC 3 cut(s) 44, 120, 221
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.