Rroxscaffold_6G00422410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009038
Physical Location & Seq
Forward (+)
43312649 .. 43314156
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422410.1

Sequence Viewer

Length: 561 bp
ATGGCTCTTATATATACGATTTTCATCAATCGTGTAGGGAACTTCAATGGTTTTGGACCTTTCGGTGAGTATATACAAGGCGAAAATGCTGCAGGGAAGCTACCAAATTTAGTCAGCTTCCTCTTCACAATCTCATATGATAATAAGCTTCCCACATCTTCTGAGAAAGGTTGGAAGCACTTGAGACGTATATTCAAATCCTTCAAGAACAGTGATGACTACCACAACCATTATTATCAATACCATGTTCACTATGATGACTACAAGAAGCAGCAGCGCCGCGGCAGCAGGGCAAACAAATCATCAACAGTTGAAGCACCAATGGTGCATCAACAGGCTGTTGCTGCACGAGCTTTTACCAGTACAAGAGAAAGAGCTCATGTTCATCGTCGTAGCAAAAATGAATCTCGGGATCGAAATGCCTTGGGAGATAATGACGAAAATGGGGAGGGTGTTGATGCTGATTGTGAGAAGTTCATAGAGTGGTTCCGCGACGGGTGGAAGCTTGAGAGACAAATTTCTGTAGAAGAGTTTCGGGACATGCTGGATCGAAGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.79

Weight (kDa)

8.91

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 150 - 184 1.5e-07 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 282, 492
AciI CCGC 3 cut(s) 280, 282, 490
AclWI GGATC 2 cut(s) 420, 555
AcsI RAATTY 2 cut(s) 106, 516
AfaI GTAC 1 cut(s) 364
AgsI TTSAA 4 cut(s) 46, 196, 205, 314
AluBI AGCT 6 cut(s) 100, 117, 148, 353, 377, 505
AluI AGCT 6 cut(s) 100, 117, 148, 353, 377, 505
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 2 cut(s) 178, 505
AlwI GGATC 2 cut(s) 420, 555
Ama87I CYCGRG 1 cut(s) 408
ApeKI GCWGC 5 cut(s) 89, 271, 274, 285, 344
ApoI RAATTY 2 cut(s) 106, 516
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 5 cut(s) 76, 283, 286, 297, 331
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BcoDI GTCTC 2 cut(s) 178, 505
BfmI CTRYAG 2 cut(s) 90, 522
BfoI RGCGCY 1 cut(s) 280
BisI GCNGC 7 cut(s) 90, 272, 275, 280, 283, 286, 345
BlsI GCNGC 7 cut(s) 91, 273, 276, 281, 284, 287, 346
Bme18I GGWCC 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 408
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 488
BmsI GCATC 2 cut(s) 337, 448
BpuEI CTTGAG 2 cut(s) 202, 527
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 280, 423
Bse1I ACTGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 280, 423
BseJI GATNNNNATC 1 cut(s) 23
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 360
BseXI GCAGC 5 cut(s) 76, 283, 286, 297, 331
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 282, 492
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 551
BsmAI GTCTC 2 cut(s) 178, 505
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 2 cut(s) 412, 547
BspACI CCGC 3 cut(s) 280, 282, 490
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 282, 492
BspLI GGNNCC 1 cut(s) 488
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 2 cut(s) 420, 555
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 2 cut(s) 280, 423
BssMI GATC 2 cut(s) 412, 547
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 212, 310
Bst6I CTCTTC 2 cut(s) 128, 522
BstDEI CTNAG 2 cut(s) 162, 558
BstDSI CCRYGG 1 cut(s) 280
BstFNI CGCG 2 cut(s) 282, 492
BstH2I RGCGCY 1 cut(s) 280
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 2 cut(s) 415, 550
BstMAI GTCTC 2 cut(s) 178, 505
BstMBI GATC 2 cut(s) 412, 547
BstMWI GCNNNNNNNGC 3 cut(s) 285, 344, 350
BstNSI RCATGY 1 cut(s) 544
BstSFI CTRYAG 2 cut(s) 90, 522
BstUI CGCG 2 cut(s) 282, 492
BstV1I GCAGC 5 cut(s) 76, 283, 286, 297, 331
BtgI CCRYGG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 56
Cfr42I CCGCGG 1 cut(s) 283
Csp6I GTAC 1 cut(s) 363
CviAII CATG 3 cut(s) 245, 380, 541
CviJI RGCY 8 cut(s) 5, 100, 117, 148, 338, 353, 377, 505
CviKI_1 RGCY 8 cut(s) 5, 100, 117, 148, 338, 353, 377, 505
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 2 cut(s) 162, 558
DpnI GATC 2 cut(s) 414, 549
DpnII GATC 2 cut(s) 412, 547
Eam1104I CTCTTC 2 cut(s) 128, 522
EarI CTCTTC 2 cut(s) 128, 522
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 3 cut(s) 248, 383, 544
FaqI GGGAC 1 cut(s) 551
FatI CATG 3 cut(s) 244, 379, 540
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 7 cut(s) 90, 272, 275, 280, 283, 286, 345
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 7 cut(s) 90, 272, 275, 280, 283, 286, 345
GlaI GCGC 1 cut(s) 278
GluI GCNGC 7 cut(s) 90, 272, 275, 280, 283, 286, 345
HaeII RGCGCY 1 cut(s) 280
HhaI GCGC 1 cut(s) 279
Hin1II CATG 3 cut(s) 248, 383, 544
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 146, 503
HinfI GANTC 1 cut(s) 404
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 205, 410, 536
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 2 cut(s) 393, 497
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 212, 310
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 3 cut(s) 92, 328, 347
HpyF10VI GCNNNNNNNGC 3 cut(s) 285, 344, 350
HpyF3I CTNAG 2 cut(s) 162, 558
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 3 cut(s) 248, 383, 544
HspAI GCGC 1 cut(s) 277
KspI CCGCGG 1 cut(s) 283
Kzo9I GATC 2 cut(s) 412, 547
LpnPI CCDG 5 cut(s) 78, 274, 320, 373, 530
Lsp1109I GCAGC 5 cut(s) 76, 283, 286, 297, 331
LweI GCATC 2 cut(s) 337, 448
MaeII ACGT 1 cut(s) 187
MalI GATC 2 cut(s) 414, 549
MboI GATC 2 cut(s) 412, 547
MboII GAAGA 3 cut(s) 115, 150, 539
MhlI GDGCHC 1 cut(s) 379
MluCI AATT 2 cut(s) 106, 516
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 2 cut(s) 131, 442
MroXI GAANNNNTTC 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 282
MvnI CGCG 2 cut(s) 282, 492
MwoI GCNNNNNNNGC 3 cut(s) 285, 344, 350
NdeI CATATG 1 cut(s) 136
NdeII GATC 2 cut(s) 412, 547
NlaIII CATG 3 cut(s) 248, 383, 544
NlaIV GGNNCC 1 cut(s) 488
NspI RCATGY 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 7 cut(s) 91, 273, 276, 281, 284, 287, 346
Psp124BI GAGCTC 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 488
PspPI GGNCC 1 cut(s) 56
PstI CTGCAG 1 cut(s) 94
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 379
SacII CCGCGG 1 cut(s) 283
SatI GCNGC 7 cut(s) 90, 272, 275, 280, 283, 286, 345
Sau3AI GATC 2 cut(s) 412, 547
Sau96I GGNCC 1 cut(s) 56
SduI GDGCHC 1 cut(s) 379
SetI ASST 9 cut(s) 61, 102, 119, 150, 172, 190, 355, 379, 507
SfaNI GCATC 2 cut(s) 337, 448
SfcI CTRYAG 2 cut(s) 90, 522
Sfr303I CCGCGG 1 cut(s) 283
SgrBI CCGCGG 1 cut(s) 283
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 255
SmlI CTYRAG 2 cut(s) 181, 506
SmoI CTYRAG 2 cut(s) 181, 506
Sse9I AATT 2 cut(s) 106, 516
SsiI CCGC 3 cut(s) 280, 282, 490
SstI GAGCTC 1 cut(s) 379
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 212, 310
TaiI ACGT 1 cut(s) 190
TaqI TCGA 2 cut(s) 415, 550
TasI AATT 2 cut(s) 106, 516
TatI WGTACW 1 cut(s) 362
TauI GCSGC 2 cut(s) 282, 285
TfiI GAWTC 1 cut(s) 404
TscAI CASTG 1 cut(s) 217
TseI GCWGC 5 cut(s) 89, 271, 274, 285, 344
TspDTI ATGAA 4 cut(s) 13, 374, 417, 466
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 2 cut(s) 106, 516
XceI RCATGY 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.