MD04G1212000.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007241
Physical Location & Seq
Forward (+)
29643064 .. 29649578
6515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1212000.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGAAGAAGAACCAAAACGGCGGCATCAAGGTCGTTCAGAGTAATCATCACCATCACGCCAATAACAATGGCTTCCTTCCGAATTCTCTCAAATTCATTTCTTCTTGTATTAAAACTGCATCTTCCGGCGTAAGGTCGGGCGCCGCCACCGTCGCCGCCTCCATCACCGCCGACCCTCACGATTGCCGAGACCAGGTGTTGTGGGCTTGCTTTGACAAACTAGAGCTTGGTCCATCTTCCTTCAAACATGTTTTGCTACTCGGTTATTCCAATGGCTTTCAAGTCCTTGATATTGAAGATGCCTCGAATTTCAGTGAACTTGTATCAAGGCGTGATGATCCAGTTACATTTTTACAGATGCAGCCCCTGCCTGCAAAGTGTGAGGGTCAAGAAGGATTCAGATCCTCACATCCTATTCTCATGGTTGTTGCATGTGACGAAAAGAGCACAGGTATGATGCAAACAGGGAGAGATGGGTTGGTCAATGGTCATTCTGAGCCTCAAACAGGAAACTCTTCTTTGTCTCCAACAGCTGTTCGGTTTTATTCGCTAAGGTCTTGCAGTTATGTTCATGTTCTAAGATTCCGTTCAACTGTGTATATGGTTAGATGCAGTCCTCAAGTAGTAGCTGTGGGTCTTGCATCACAAATATACTGCTTTGATGCTGTCACTCTTGAGAACAAATTCAGTGTCCTCACTTATCCTGTCCCCCAATTGGGAGTTCAAGGACTGGTTGGGGTCAATATCGGGTATGGTCCAATGGCTGTTGGTCCCAGGTGGTTGGCTTATGCTTCCAACAACCCTCTGATGTCAAACACAGGCCGCCTCAGTCCTCAAAGTCTTACTCCTCCTGGTGTAAGTCCATCAACTTCGCCAAGTAGTGGGAATCTGATGGCTCGATATGCAATGGAATCTAGTAAACAGTTAGCTTCTGGGTTACTTAATCTTGGAGACATGGGTTACAAAACTTTGTCCAAGTACTATCAAGATCTTGTACCAGATGGTTCTAGCTCTCCTGTGTCATCAAATTCTAGCTGGAAAGTTGGGAGGGTTGCATCACATTCAACCGAAGCAGATATTGCTGGAATGGTTGTTATAAAAGACTTTGTTTCCAGAGCCATTGTATCACAGTTCAGGGCACATACTAGTCCCATTTCTGCCCTGTGTTTTGACCCAAGTGGGACTCTTCTAGTTACTGCCTCAATACATGGAAACAATATAAACATTTTTCGAATAATGCCATCCTGCTCGCGTAATGGATCAGGCACTCAAAGCTATGACTGGACTTCTTCTCATGTGCACCTTTACAAACTACATCGTGGCATAACATCTGCTGTGATCCAAGACATTTGTTTTAGTCAATACAGTCAATGGATCACTATTGTTTCATCCAGGGGGACTAGCCATATTTTTGCTTTGTCTCCATTCGGTGGTGAAACTGTTCTTCAAATACAGAATTCTCATGTTGACGGATCTACTCTTTCACCGATTCCATCTGCACCATGGTGGTTTACTCCATATTTCACAAGAACTCAGCAACCCTTTTCTCCACCTCCAGCAGCTACCCTTTCTGTAGTTAGCAGAATAAAAAATAACTCCGGGTGGCTCAACACTGTTAGTAATGCTGCATCTTCTGCAGCAGGAAAGGCTTCCTTTCGGTCTGGGGCTGTTGCCGCTGTATTCCATAGTTCTGTACCTCATGATATGCAATCTTCCCATGCGAAGGTCACTGCATTGGAGCACGTATTGGCTTATACTGCTTCTGGTCATGTAATTCAGTATAAACTACTGCCTTCATTGGGAGGAGAGCCAGGCGACGCTGCTTCGAGAGCAGGGCCAGGTTCTTCAGTACAGATACAGGATGAAGATTTGCGAGTGAAAGTTGAATCTCTTCATTTTTTGGATGTTTGCCGAAGAAATGATTGGCCTGAAAGAGAGGAATGCATTTCTGGGGTTATACTTGGGAAACAAGAAGATATTGACACAATTGATAGTTCTGACTGTGATGATAGTGATGTTGGGGATAAGGAGTTGGCAAAACCCCTTGAACGATCTCATGTGTATCTCTCCAATGCTGAGGTTCAGATCAATTCCGGAAGGATTCCAATCTGGCAAAAGTCGAAGATATATTTCTATACAATGAGTCCATGTGCTGCTGGTGAGCTAAATTTTAGTAAAGATCTTACCCGCGGAGAGATGGAAATAGAGAAGGTTCCTGGTCAAGAGGTTGAAATGAGGCGGAAGGATTTGCTACCTGTTGTTCATCCCTTTCATAGATTTCGGTCTGACTGGAATGGCAGGCATCTACTTGGAGGAGGATCCTCGAGTTCATCTTCTGATTCTCATGAAGATAAAGAAAAGTTTCAGGAAAATTCTGGTATTTCTGGTGAGAATTTGACACCTATTGGCTCAGCTGAAAATGGTAACCCAGATGCTGGTAATTCCTATCCATATATTCTCCAACCTGGAAATGGACAGAATGGCGAAAAAAGAGGGAGATCATTTTCAGTATCACCTCACTTGAACCATATATCCACAAAGAAAAATATTACTTCAGTATCCCTGAAACAACCAACCTCAGGTGTTTCTACTGGTGAGGATAGTAATTTTTCAAAATGTCTATCCACTTTAACAAGCGGCTCCCTTTCAGCTGATAGAACAATTGCAACAAGGGTTCGGTCGGTGAATAGTGGTGGGGCCAGTAAAGGTTCCAATGTAAGTTCTAACCATTCTGATCTTAGTATGAACATGCTCGATGAGGGACCAGTACATGAATCACCGGATTTTGAGCCTTTTTTCCAAGAGGGGTATTGTAAAGCATCACCTCTGAGCAACTTTCGTGAGTCGACTGAAGGTGTAACTGATGTTGACAGCCCCTGTGATAGGGAGAAATGCGAGGAAGATGGTGATAGTGATGACATGCTTGGTGGTGTATTTGCCTTCTCCGAGGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

986

Amino Acids

106.34

Weight (kDa)

6.11

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 84 - 485 1.1e-36 BCAS3, WD40 repeat
BCAS3 PF12490 617 - 753 3.6e-38 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 3 cut(s) 881, 1430, 2435
AccI GTMKAC 1 cut(s) 2846
AccII CGCG 2 cut(s) 1252, 2190
AccIII TCCGGA 1 cut(s) 2093
AclWI GGATC 8 cut(s) 332, 396, 1267, 1333, 1382, 1480, 2313, 2326
AcsI RAATTY 9 cut(s) 82, 92, 307, 683, 1027, 1456, 2167, 2371, 2392
AcuI CTGAAG 3 cut(s) 1830, 2538, 2871
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 5 cut(s) 980, 996, 1695, 1851, 2769
AflIII ACRYGT 1 cut(s) 247
AhlI ACTAGT 1 cut(s) 1145
AjnI CCWGG 8 cut(s) 192, 773, 850, 1391, 1810, 1837, 2215, 2464
AjuI GAANNNNNNNTTGG 4 cut(s) 705, 737, 1906, 1938
AleI CACNNNNGTG 1 cut(s) 1504
AloI GAACNNNNNNTCC 2 cut(s) 2516, 2548
Alw21I GWGCWC 3 cut(s) 449, 1302, 1743
Alw26I GTCTC 4 cut(s) 183, 528, 945, 1425
Alw44I GTGCAC 1 cut(s) 1298
AlwI GGATC 8 cut(s) 332, 396, 1267, 1333, 1382, 1480, 2313, 2326
AlwNI CAGNNNCTG 3 cut(s) 367, 2435, 2877
Ama87I CYCGRG 1 cut(s) 2323
Aor13HI TCCGGA 1 cut(s) 2093
AoxI GGCC 4 cut(s) 820, 1835, 1925, 2697
ApaLI GTGCAC 1 cut(s) 1298
ApeKI GCWGC 6 cut(s) 361, 1559, 1625, 1637, 1820, 2153
ApoI RAATTY 9 cut(s) 82, 92, 307, 683, 1027, 1456, 2167, 2371, 2392
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
Asp700I GAANNNNTTC 6 cut(s) 514, 683, 1440, 1648, 1890, 2361
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 6 cut(s) 230, 755, 770, 1835, 2697, 2762
AsuC2I CCSGG 1 cut(s) 1600
AsuII TTCGAA 1 cut(s) 1231
AvaI CYCGRG 1 cut(s) 2323
AvaII GGWCC 4 cut(s) 230, 755, 770, 2762
AxyI CCTNAGG 1 cut(s) 2578
BaeGI GKGCMC 2 cut(s) 1141, 1302
BaeI ACNNNNGTAYC 2 cut(s) 2541, 2574
BamHI GGATCC 1 cut(s) 2318
BanI GGYRCC 1 cut(s) 140
Bbv12I GWGCWC 3 cut(s) 449, 1302, 1743
BbvCI CCTCAGC 1 cut(s) 2076
BbvI GCAGC 6 cut(s) 373, 1571, 1612, 1649, 1807, 2140
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 8 cut(s) 194, 775, 852, 1393, 1812, 1839, 2217, 2466
BciVI GTATCC 1 cut(s) 2569
BcnI CCSGG 1 cut(s) 1600
BcoDI GTCTC 4 cut(s) 183, 528, 945, 1425
BcuI ACTAGT 1 cut(s) 1145
BfaI CTAG 7 cut(s) 221, 915, 1008, 1032, 1146, 1190, 1401
BfmI CTRYAG 2 cut(s) 1572, 1635
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 1 cut(s) 2569
BglII AGATCT 2 cut(s) 988, 2179
BlpI GCTNAGC 1 cut(s) 2410
BmcAI AGTACT 1 cut(s) 980
Bme1390I CCNGG 9 cut(s) 194, 775, 852, 1393, 1600, 1812, 1839, 2217, 2466
Bme18I GGWCC 4 cut(s) 230, 755, 770, 2762
BmeT110I CYCGRG 1 cut(s) 2323
BmgT120I GGNCC 6 cut(s) 230, 755, 770, 1835, 2697, 2762
BmiI GGNNCC 8 cut(s) 142, 772, 2214, 2320, 2641, 2698, 2710, 2763
BmrFI CCNGG 9 cut(s) 194, 775, 852, 1393, 1600, 1812, 1839, 2217, 2466
BpmI CTGGAG 1 cut(s) 1539
Bpu10I CCTNAGC 2 cut(s) 551, 2076
Bpu1102I GCTNAGC 1 cut(s) 2410
Bpu14I TTCGAA 1 cut(s) 1231
BpuEI CTTGAG 2 cut(s) 603, 695
BpuMI CCSGG 1 cut(s) 1600
BsaAI YACGTR 1 cut(s) 1744
BsaBI GATNNNNATC 2 cut(s) 400, 2105
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 773, 1392, 1502, 2188, 2946
BsaWI WCCGGW 2 cut(s) 2093, 2779
Bse1I ACTGG 7 cut(s) 341, 735, 1286, 2294, 2596, 2700, 2765
Bse21I CCTNAGG 1 cut(s) 2578
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 2 cut(s) 400, 2105
BseAI TCCGGA 1 cut(s) 2093
BseBI CCWGG 8 cut(s) 194, 775, 852, 1393, 1812, 1839, 2217, 2466
BseDI CCNNGG 5 cut(s) 773, 1392, 1502, 2188, 2946
BseGI GGATG 6 cut(s) 409, 1241, 1388, 1867, 1909, 2263
BseJI GATNNNNATC 2 cut(s) 400, 2105
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 7 cut(s) 486, 841, 1547, 2067, 2424, 2592, 2819
BseNI ACTGG 7 cut(s) 341, 735, 1286, 2294, 2596, 2700, 2765
BseRI GAGGAG 3 cut(s) 837, 1818, 2328
BseSI GKGCMC 2 cut(s) 1141, 1302
BseXI GCAGC 6 cut(s) 373, 1571, 1612, 1649, 1807, 2140
BsgI GTGCAG 1 cut(s) 1482
Bsh1236I CGCG 2 cut(s) 1252, 2190
Bsh1285I CGRYCG 1 cut(s) 2681
BshFI GGCC 4 cut(s) 822, 1837, 1927, 2699
BshNI GGYRCC 1 cut(s) 140
BsiEI CGRYCG 1 cut(s) 2681
BsiHKAI GWGCWC 3 cut(s) 449, 1302, 1743
BsiHKCI CYCGRG 1 cut(s) 2323
BsiSI CCGG 4 cut(s) 126, 1599, 2094, 2780
BslFI GGGAC 6 cut(s) 692, 756, 1134, 1195, 1411, 2775
BsmAI GTCTC 4 cut(s) 183, 528, 945, 1425
BsmFI GGGAC 6 cut(s) 692, 756, 1134, 1195, 1411, 2775
BsmI GAATGC 1 cut(s) 1946
BsnI GGCC 4 cut(s) 822, 1837, 1927, 2699
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 2323
Bsp119I TTCGAA 1 cut(s) 1231
Bsp1286I GDGCHC 4 cut(s) 449, 1141, 1302, 1743
Bsp13I TCCGGA 1 cut(s) 2093
Bsp1720I GCTNAGC 1 cut(s) 2410
Bsp19I CCATGG 1 cut(s) 1502
BspANI GGCC 4 cut(s) 822, 1837, 1927, 2699
BspCNI CTCAG 7 cut(s) 487, 840, 1546, 2068, 2423, 2591, 2820
BspEI TCCGGA 1 cut(s) 2093
BspFNI CGCG 2 cut(s) 1252, 2190
BspHI TCATGA 2 cut(s) 1699, 2344
BspLI GGNNCC 8 cut(s) 142, 772, 2214, 2320, 2641, 2698, 2710, 2763
BspMAI CTGCAG 1 cut(s) 1639
BspPI GGATC 8 cut(s) 332, 396, 1267, 1333, 1382, 1480, 2313, 2326
BspT104I TTCGAA 1 cut(s) 1231
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 7 cut(s) 341, 735, 1286, 2294, 2596, 2700, 2765
BssECI CCNNGG 5 cut(s) 773, 1392, 1502, 2188, 2946
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 1502
Bst2UI CCWGG 8 cut(s) 194, 775, 852, 1393, 1812, 1839, 2217, 2466
Bst4CI ACNGT 8 cut(s) 151, 595, 924, 1131, 1367, 1441, 1615, 2003
Bst6I CTCTTC 3 cut(s) 520, 1191, 1896
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 3 cut(s) 367, 1079, 1634
BstBAI YACGTR 1 cut(s) 1744
BstBI TTCGAA 1 cut(s) 1231
BstC8I GCNNGC 4 cut(s) 208, 372, 1250, 2300
BstDSI CCRYGG 2 cut(s) 1502, 2188
BstEII GGTNACC 1 cut(s) 2423
BstENI CCTNNNNNAGG 2 cut(s) 504, 2881
BstF5I GGATG 6 cut(s) 409, 1241, 1388, 1867, 1909, 2263
BstFNI CGCG 2 cut(s) 1252, 2190
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstMAI GTCTC 4 cut(s) 183, 528, 945, 1425
BstMCI CGRYCG 1 cut(s) 2681
BstNI CCWGG 8 cut(s) 194, 775, 852, 1393, 1812, 1839, 2217, 2466
BstNSI RCATGY 4 cut(s) 251, 435, 2752, 2923
BstPI GGTNACC 1 cut(s) 2423
BstSCI CCNGG 9 cut(s) 192, 773, 850, 1391, 1598, 1810, 1837, 2215, 2464
BstSFI CTRYAG 2 cut(s) 1572, 1635
BstSLI GKGCMC 2 cut(s) 1141, 1302
BstUI CGCG 2 cut(s) 1252, 2190
BstV1I GCAGC 6 cut(s) 373, 1571, 1612, 1649, 1807, 2140
BstX2I RGATCY 5 cut(s) 401, 988, 1472, 2179, 2318
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 5 cut(s) 401, 988, 1472, 2179, 2318
Bsu36I CCTNAGG 1 cut(s) 2578
BsuI GTATCC 1 cut(s) 2569
BsuRI GGCC 4 cut(s) 822, 1837, 1927, 2699
BtgI CCRYGG 2 cut(s) 1502, 2188
BtsCI GGATG 6 cut(s) 409, 1241, 1388, 1867, 1909, 2263
BtsI GCAGTG 1 cut(s) 1728
BtsIMutI CAGTG 4 cut(s) 319, 694, 1611, 1728
Cac8I GCNNGC 4 cut(s) 208, 372, 1250, 2300
CaiI CAGNNNCTG 3 cut(s) 367, 2435, 2877
CciI TCATGA 2 cut(s) 1699, 2344
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 6 cut(s) 230, 755, 770, 1835, 2697, 2762
Cfr42I CCGCGG 1 cut(s) 2191
CseI GACGC 1 cut(s) 1826
CsiI ACCWGGT 1 cut(s) 192
Csp6I GTAC 5 cut(s) 979, 995, 1694, 1850, 2768
CviQI GTAC 5 cut(s) 979, 995, 1694, 1850, 2768
DinI GGCGCC 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 520, 1191, 1896
EarI CTCTTC 3 cut(s) 520, 1191, 1896
EciI GGCGGA 1 cut(s) 2254
Eco130I CCWWGG 1 cut(s) 1502
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 4 cut(s) 230, 755, 770, 2762
Eco57I CTGAAG 3 cut(s) 1830, 2538, 2871
Eco81I CCTNAGG 1 cut(s) 2578
Eco88I CYCGRG 1 cut(s) 2323
Eco91I GGTNACC 1 cut(s) 2423
EcoNI CCTNNNNNAGG 2 cut(s) 504, 2881
EcoO65I GGTNACC 1 cut(s) 2423
EcoRI GAATTC 2 cut(s) 82, 1456
EcoRII CCWGG 8 cut(s) 192, 773, 850, 1391, 1810, 1837, 2215, 2464
EcoT14I CCWWGG 1 cut(s) 1502
EcoT22I ATGCAT 1 cut(s) 1946
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 1502
FalI AAGNNNNNCTT 2 cut(s) 1637, 1669
FaqI GGGAC 6 cut(s) 692, 756, 1134, 1195, 1411, 2775
FauI CCCGC 1 cut(s) 2195
FblI GTMKAC 1 cut(s) 2846
FokI GGATG 6 cut(s) 396, 1228, 1375, 1874, 1916, 2250
FspBI CTAG 7 cut(s) 221, 915, 1008, 1032, 1146, 1190, 1401
GlaI GCGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 1539
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 4 cut(s) 822, 1837, 1927, 2699
HapII CCGG 4 cut(s) 126, 1599, 2094, 2780
HgaI GACGC 1 cut(s) 1826
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 141
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 3 cut(s) 1468, 2847, 2869
HindII GTYRAC 3 cut(s) 1468, 2847, 2869
HpaII CCGG 4 cut(s) 126, 1599, 2094, 2780
Hpy166II GTNNAC 7 cut(s) 317, 920, 1300, 1468, 1512, 2847, 2869
Hpy8I GTNNAC 7 cut(s) 317, 920, 1300, 1468, 1512, 2847, 2869
Hpy99I CGWCG 2 cut(s) 155, 1820
HpyCH4III ACNGT 8 cut(s) 151, 595, 924, 1131, 1367, 1441, 1615, 2003
HpyCH4IV ACGT 1 cut(s) 1743
HpySE526I ACGT 1 cut(s) 1743
Hsp92I GRCGYC 1 cut(s) 141
HspAI GCGC 1 cut(s) 141
KasI GGCGCC 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 2093
KspI CCGCGG 1 cut(s) 2191
LmnI GCTCC 2 cut(s) 1738, 2645
Lsp1109I GCAGC 6 cut(s) 373, 1571, 1612, 1649, 1807, 2140
MabI ACCWGGT 1 cut(s) 192
MaeI CTAG 7 cut(s) 221, 915, 1008, 1032, 1146, 1190, 1401
MaeII ACGT 1 cut(s) 1743
MaeIII GTNAC 9 cut(s) 343, 434, 667, 936, 959, 1192, 1726, 2423, 2857
MfeI CAATTG 3 cut(s) 713, 1986, 2661
MflI RGATCY 5 cut(s) 401, 988, 1472, 2179, 2318
MhlI GDGCHC 4 cut(s) 449, 1141, 1302, 1743
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 3 cut(s) 1177, 2152, 2852
MmeI TCCRAC 3 cut(s) 551, 819, 2485
Mph1103I ATGCAT 1 cut(s) 1946
MroI TCCGGA 1 cut(s) 2093
MroXI GAANNNNTTC 6 cut(s) 514, 683, 1440, 1648, 1890, 2361
MseI TTAA 3 cut(s) 111, 942, 2630
MslI CAYNNNNRTG 2 cut(s) 452, 1504
MspA1I CMGCKG 5 cut(s) 533, 1676, 2190, 2414, 2651
MspI CCGG 4 cut(s) 126, 1599, 2094, 2780
MspR9I CCNGG 9 cut(s) 194, 775, 852, 1393, 1600, 1812, 1839, 2217, 2466
MunI CAATTG 3 cut(s) 713, 1986, 2661
Mva1269I GAATGC 1 cut(s) 1946
MvaI CCWGG 8 cut(s) 194, 775, 852, 1393, 1812, 1839, 2217, 2466
MvnI CGCG 2 cut(s) 1252, 2190
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 1600
NcoI CCATGG 1 cut(s) 1502
NlaIV GGNNCC 8 cut(s) 142, 772, 2214, 2320, 2641, 2698, 2710, 2763
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 3 cut(s) 434, 667, 1726
NsiI ATGCAT 1 cut(s) 1946
NspI RCATGY 4 cut(s) 251, 435, 2752, 2923
NspV TTCGAA 1 cut(s) 1231
OliI CACNNNNGTG 1 cut(s) 1504
PaeR7I CTCGAG 1 cut(s) 2323
PagI TCATGA 2 cut(s) 1699, 2344
PciI ACATGT 1 cut(s) 247
PctI GAATGC 1 cut(s) 1946
PdmI GAANNNNTTC 6 cut(s) 514, 683, 1440, 1648, 1890, 2361
PfeI GAWTC 9 cut(s) 396, 582, 886, 911, 1489, 1886, 2101, 2339, 2774
PflMI CCANNNNNTGG 3 cut(s) 881, 1430, 2435
PleI GAGTC 3 cut(s) 1177, 2151, 2851
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 3 cut(s) 1177, 2151, 2851
Ppu21I YACGTR 1 cut(s) 1744
PscI ACATGT 1 cut(s) 247
PsiI TTATAA 1 cut(s) 1097
Psp6I CCWGG 8 cut(s) 192, 773, 850, 1391, 1810, 1837, 2215, 2464
PspEI GGTNACC 1 cut(s) 2423
PspGI CCWGG 8 cut(s) 192, 773, 850, 1391, 1810, 1837, 2215, 2464
PspN4I GGNNCC 8 cut(s) 142, 772, 2214, 2320, 2641, 2698, 2710, 2763
PspPI GGNCC 6 cut(s) 230, 755, 770, 1835, 2697, 2762
PspXI VCTCGAGB 1 cut(s) 2323
PstI CTGCAG 1 cut(s) 1639
PstNI CAGNNNCTG 3 cut(s) 367, 2435, 2877
PsuI RGATCY 5 cut(s) 401, 988, 1472, 2179, 2318
PvuII CAGCTG 3 cut(s) 533, 2414, 2651
RsaI GTAC 5 cut(s) 980, 996, 1695, 1851, 2769
RsaNI GTAC 5 cut(s) 979, 995, 1694, 1850, 2768
RseI CAYNNNNRTG 2 cut(s) 452, 1504
SacII CCGCGG 1 cut(s) 2191
SalI GTCGAC 1 cut(s) 2845
SaqAI TTAA 3 cut(s) 111, 942, 2630
Sau96I GGNCC 6 cut(s) 230, 755, 770, 1835, 2697, 2762
ScaI AGTACT 1 cut(s) 980
SchI GAGTC 3 cut(s) 1177, 2152, 2852
ScrFI CCNGG 9 cut(s) 194, 775, 852, 1393, 1600, 1812, 1839, 2217, 2466
SduI GDGCHC 4 cut(s) 449, 1141, 1302, 1743
SexAI ACCWGGT 1 cut(s) 192
SfcI CTRYAG 2 cut(s) 1572, 1635
SfoI GGCGCC 1 cut(s) 142
Sfr274I CTCGAG 1 cut(s) 2323
Sfr303I CCGCGG 1 cut(s) 2191
SfuI TTCGAA 1 cut(s) 1231
SgrBI CCGCGG 1 cut(s) 2191
SinI GGWCC 4 cut(s) 230, 755, 770, 2762
SlaI CTCGAG 1 cut(s) 2323
SmiMI CAYNNNNRTG 2 cut(s) 452, 1504
SmlI CTYRAG 3 cut(s) 618, 674, 2323
SmoI CTYRAG 3 cut(s) 618, 674, 2323
SpeI ACTAGT 1 cut(s) 1145
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 2548
SspMI CTAG 7 cut(s) 221, 915, 1008, 1032, 1146, 1190, 1401
StyD4I CCNGG 9 cut(s) 192, 773, 850, 1391, 1598, 1810, 1837, 2215, 2464
StyI CCWWGG 1 cut(s) 1502
TaaI ACNGT 8 cut(s) 151, 595, 924, 1131, 1367, 1441, 1615, 2003
TaiI ACGT 1 cut(s) 1746
TaqI TCGA 8 cut(s) 305, 898, 1231, 1826, 2120, 2324, 2754, 2846
TaqII GACCGA 3 cut(s) 1647, 2271, 2667
TatI WGTACW 3 cut(s) 978, 1849, 2767
TauI GCSGC 6 cut(s) 24, 146, 158, 825, 1676, 2640
TfiI GAWTC 9 cut(s) 396, 582, 886, 911, 1489, 1886, 2101, 2339, 2774
Tru1I TTAA 3 cut(s) 111, 942, 2630
Tru9I TTAA 3 cut(s) 111, 942, 2630
TscAI CASTG 4 cut(s) 319, 694, 1618, 1735
TseFI GTSAC 3 cut(s) 434, 667, 1726
TseI GCWGC 6 cut(s) 361, 1559, 1625, 1637, 1820, 2153
Tsp45I GTSAC 3 cut(s) 434, 667, 1726
TspGWI ACGGA 2 cut(s) 575, 1485
TspRI CASTG 4 cut(s) 319, 694, 1618, 1735
Van91I CCANNNNNTGG 3 cut(s) 881, 1430, 2435
VneI GTGCAC 1 cut(s) 1298
VpaK11BI GGWCC 4 cut(s) 230, 755, 770, 2762
XagI CCTNNNNNAGG 2 cut(s) 504, 2881
XapI RAATTY 9 cut(s) 82, 92, 307, 683, 1027, 1456, 2167, 2371, 2392
XceI RCATGY 4 cut(s) 251, 435, 2752, 2923
XcmI CCANNNNNNNNNTGG 2 cut(s) 1500, 2468
XhoI CTCGAG 1 cut(s) 2323
XmiI GTMKAC 1 cut(s) 2846
XmnI GAANNNNTTC 6 cut(s) 514, 683, 1440, 1648, 1890, 2361
XspI CTAG 7 cut(s) 221, 915, 1008, 1032, 1146, 1190, 1401
ZrmI AGTACT 1 cut(s) 980
Zsp2I ATGCAT 1 cut(s) 1946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.