Rw3G004040

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007241
Physical Location & Seq
Reverse (-)
3462098 .. 3474371
12274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004040.1

Sequence Viewer

Length: 4419 bp
ATGGCAAATGGAGAAGTGCAGGGACCTTATTGTCCATGGGGGCCTGGTAAGAACCTTGAAAGAATAGAAGTGAATGATCCTAATGAGGTTTCTGTGGTAATACCTCAAAGAAGAGGTGGTCTACGCCATTCTGTTTCGGCCCAAGATTTGAGATATAGGGATATAGAAAAGGAGAAACATGATGGCCAATCTCCTAGAGGAGTTTTGGAAGCTTGCGTGGAAAGCTTTGAATCTGAAACTAGCGTTTCTGAAAACAGCAAATCAGAAGCAGAAGCTTCTTGTCCAGATTCAAAGCCTCAATCTCACTGGTGTAGATTTTTTAAGCTGTGGAGTAAAAGATCAATCAAGCAGCACTTCTTCCCTCTTGGTGTATCCAAGATGTCAATAAAAAGAAGGAGCAACAGTACAAAAGACAATTCAGTACTGACTAATATCTACAACTTCAAGTCTCCATTAAAGAGTTTCTCCTTCTCTGCACTCCAAACCGCCACCAATAATTTCAACAATGAAAATATAATTGGAAAGGGTGGCTATTCCGAAGTATATAGGGGGACTTTAAAGGATGGGCAACTAGTAGCAGTAAAGCGGCTGACTAATGGAACATCCGATGAGAAAACTGCAGGCTTTCTATCTGAACTTGGCATTATAGCTCATGTAGATCATCCTAATACTGCTAAGCTAATTGGCTGTTGCGTTGAAAAAGGAATGCACCTTGTTTTTCAATTATCTCCACTTGGGAGTTTAGGATCTCTCCTGCATGGTCCAAAGAATTATATTCTTGATTGGAGTAAAAGGTATAAAATTGCGTTAGGGGCAGCAGATGGTCTGCTGTATCTTCATGATAGTTGTCAGAGGCGAATCATCCATAGAGATATCAAGGCTGATAATATTCTACTTACAGAGGATTTTGTGCCTCAGATATGTGACTTTGGGCTTGCAAAGTGGCTACCAAAACAATGGACTCATCACAACGTCTCTAAGTTTGAGGGCACATTTGGGTATTTTGCTCCTGAATATTTCATGCATGGGATTGTAGATGAAAAGACTGATGTTTATTCTTTTGGAGTCCTACTCTTGGAGCTTATAACTGGGCGTCCAGCTTTGGATAATCTTCAGCAAAGCCTTGTAATTTGGGCAAAGCCCTTGCTTAATAAAAATGACGTCAAGGAGCTTGTTGATCCTTCTATTGGTGACAACTATGACAGCACAGAGATGGATCATATGATTTTGACTGCATCTTTGTGCATAGAGCAGTCTTCTATTCTACGTCCTCGCATGAGTCAGGTTGTGGTACTGCTAAGAGGTGACGAGTATGTCTCCAAGTGTGCAAAAGAAACGAAAAGGAGGTCCCTGCTGAGAACATACTCGGAAGAACTGATGGATGCACAAGAATATAACTCAACAAACAATAATGGCTTCCTTCCTAATTCTCTGAAATTCATTTCCTCTTGCATTAAGACCGCCTCCTCCGGCGTCCGGTCGGCCAGCGCCTCCGTCGCCGCTTCCATCTCCGCTGACCCGCACGAGTCCCGAGACCAGGTGTTGTGGGCATGCTTTGACAGACTAGATCTTGGTCCATCTTCCTTCAAACATGTTCTGCTACTCGGTTATGTCAATGGCTTTCAAGTCCTTGATGTTGAAGATGCCTCTGATGTCAGTGAGCTTGTATCAAAACGTGATGATCCAGTCACATTTTTACAGATCCAGCCCCTACCTAGAATCTCTCAAGGTCCTGAAGGATTCAGATCTTCGCATCCATTACTCATGGTTGTTGCATGTGAAGAATCAAAGAACTCTGGTATGACACAAAATGGAAGAGATGGTTTGGTAAATGGTTATACTGAGCCTCAAACAAGCAACTCTGCTATGTCTCCTAGAGTTGTTCGGTTTTATTCACTAAGGTCTCACAGTTATGTTCATGTTCTGAGATTCCGCTCGACTGTGTATATGGTTAGATGCAGTCCTCAGATAGTGGCTGTTGGTCTTGCCTCTCAAATATACTGCTTTGATGCTGTCACTCTAGAGAACAAGTTCAGTGTTCTTACTTATCCTGTCCCCCAGTTGGGAGTCCAAGGACTGGTTGGGGTTAATATTGGATATGGTCCCATGGCTGTTGGTCCCAGGTGGTTGGCTTATGCCTCCAACAACCCTTTACTGTCAAACACTTGCCGACTAAGCCCGCAAAGTCTTACACCTCCAGGTGTCAGTCCATCAACTTCACCAAGTAATGGAAATCTGGTGGCTCGGTATGCCATGGAATCTAGTAAGCAGTTAGCTGCTGGGTTGCTAAATCTGGGAGACATGGGCTACAAAACTTTGTCCAAGTACTATCAACCAGATGGTTCCAGCTCTCCAGTGTCATCAAATTCTATCTGGAAAGTTGGGAAGGTTGGATCACATTCAACAGAAACAGATATTGCTGGAATGGTTGTTGTCAAAGACATTGTTTCCAGGGCGGTCATTTCACAATTTAGGGCACATACTAGTCCCATTTCTGCTCTATGTTTTGACCCAAGTGGGACTCTCCTTGTTACTGCCTCGATACATGGAAACAATATTAACATTTTTCGGATAATGCCATCACGTATTGGATCTGGCACCCAGAACTATGACTTGAATTCCTCTCATGTGCACCTTTACAAGCTCCATCGTGGCATGACATCAGCTGTAATCCAAGACATATGTTTTAGTCAATACAGTCAATGGGTTACAATTGTTTCATCGAGGGGGACTTGCCATATTTTTACTTTTTCCCCCTTTGGTGGTGATACTACTCACAAGCAGAGTTCTCATGTTGATGGGCCTAGTCATTTGCCAGTTCCGTCTGTACCGTGGTGGTTCACTCCTTATTTCCTGACAAACCAGCAACTTTTTTCTCCACCACCACCCCCTGTTACCCTTTCTGTGGTTAGCAGAATAAAAGATAACTCTGGGTGGATCAATACAGTTAGTCATGCTGCGTCTTCTGCAGCCAGAAAAGCTTCCATTCCATCTGGTGCTGTTACTGCTGTATTTCATAATTCTGTTGCTCATGATCTGCAAACTTCTCATTTAAAGGTAACAGCTCTGGAGCACCTATTGGTTTACACTCCTTCTGGTCATGCGGTACAGTTTAAACTACTTCCAAGAGTTGGGATAGAGCCTGGTGAAGCTACATCAAGAACAGTGCCAGGCTCTTCAGTACAAATACAGGATGAGGAATTGCGAGTGAAAGTTGAACCTCTGCAGTGGTGGGATGTTTGCCGAAGAACTGATTGGCCTGAAAGAGAGGAATGCATTTCTGGGATTAAACTTGGGAGACAAGAAGATGAGGAGACAGTCATGGATACTTTTGATTGTGATGTTAATGATATTGGGGATAAGGAGTCGGTGAAACCGCTTGAGCGATCTCACTTGTATCTCGCCAGCGCTGAGGTTCGGATTAACTGTGGAAGGATTCCAATCTGGCAAAAGTCTAAGATATATTTCTATACAATGAGTACCCTGGGAGCAATTGAACAAAATTTTACTAAAGATCTTACTGGAGGAGAGATTGAAATAGAAAAGGTTCCTGTTCACGAGGTCGAAATTAGGCGGAAGGATTTGCTACCTGTTTCTGATCAATTCCAAAGAGTGCAGTCTAGTTGGAGTGACAGGGATCTTATTGGAAGAGGATTCTCAAGTTCATCTTCTGATTCTCATGAAGCTAAAGAAAAATTTCTGGAAAATGTTGATATTTCTGGTGACAAGTTGGCATCAACTGGCAACCCAGATGCCGGTGGTTCTTATCCATCCATTCTCCAATCTGGGAATGGAAATAATGGGGAAACAAGAGGGAGATCTTTTTTGGCGTCACCTCTACTGAACCAAAGCTCCATAAAGAATAATATTGTTACAATACCTTCAAAACAACCGACTTCAGGTGTTTCTCTGGTTAAGGATAGTAATTTTTCAAAGAGTCCATCCACTTTAACAGGTGGTTCCCTTGCTGCTGATAGAACTATTGCACAAGAGGTTCATTTGGTGAATAGTGGTGGGGCCAATGAAAGTTCAAATGTCAGTTCTAACCGTTCTGATCTGAGTATGAACATCCTTGATGAGGGACCAGTACATGACTCACTGGATTTTGAGCAGTTTTTCCAAGAGGGGTATTGTAAAGCATCACCTTTGAGCAACTTTCCTGAATCAACTGAAGTTGTAACTGATGTGGACAGTAGCAACAGCCCCTGTGATAGGGAGAAATGCGAGGAAGATGGCGATGATGATGACATGCTTGGCGGTGTATTTGACTTCTCCGAGGAAGGTGAGAATATTTCACAACATGAACTCCTTGTTCACTTATATGTAAATTTCCTTTTCTTTGACATATTAATAGTAAATGATGGCTTAATCATTTATTCCTTTTCTGGATACAATCAAATAATATTGACTACTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

1472

Amino Acids

162.27

Weight (kDa)

5.92

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 167 - 373 2.4e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 170 - 377 6.5e-41 Protein tyrosine and serine/threonine kinase
BCAS3_WD40 PF21034 532 - 925 2.4e-35 BCAS3, WD40 repeat
BCAS3 PF12490 1061 - 1198 1.7e-45 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
AasI GACNNNNNNGTC 2 cut(s) 30, 1313
AatII GACGTC 1 cut(s) 1164
AccB1I GGYRCC 1 cut(s) 2597
AccB7I CCANNNNNTGG 3 cut(s) 2077, 2228, 3131
AccBSI CCGCTC 1 cut(s) 1935
AccI GTMKAC 1 cut(s) 121
AcoI YGGCCR 2 cut(s) 184, 1482
AcsI RAATTY 6 cut(s) 1436, 2365, 2617, 3502, 3695, 4327
AcuI CTGAAG 5 cut(s) 1097, 1755, 3162, 3882, 4191
AcyI GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
AfaI GTAC 9 cut(s) 406, 423, 1293, 2327, 2829, 3108, 3183, 3481, 4089
AfeI AGCGCT 1 cut(s) 3409
AflIII ACRYGT 1 cut(s) 1591
AhlI ACTAGT 2 cut(s) 571, 2483
AjnI CCWGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 3238, 3270
AloI GAACNNNNNNTCC 2 cut(s) 3836, 3868
Alw21I GWGCWC 2 cut(s) 2634, 3075
Alw26I GTCTC 9 cut(s) 453, 979, 1321, 1527, 1875, 1908, 2292, 3292, 3308
Alw44I GTGCAC 1 cut(s) 2630
AlwNI CAGNNNCTG 1 cut(s) 4206
Ama87I CYCGRG 1 cut(s) 1530
Aor51HI AGCGCT 1 cut(s) 3409
AoxI GGCC 7 cut(s) 41, 138, 184, 1482, 2801, 3257, 4017
ApaLI GTGCAC 1 cut(s) 2630
ApeKI GCWGC 6 cut(s) 349, 815, 2276, 2957, 2969, 3968
ApoI RAATTY 6 cut(s) 1436, 2365, 2617, 3502, 3695, 4327
ArsI GACNNNNNNTTYG 4 cut(s) 1222, 1254, 1331, 1363
AseI ATTAAT 1 cut(s) 4349
Asp700I GAANNNNTTC 4 cut(s) 2030, 2980, 3546, 3696
AspLEI GCGC 2 cut(s) 1490, 3410
AvaI CYCGRG 1 cut(s) 1530
AvaII GGWCC 8 cut(s) 23, 761, 1347, 1574, 1730, 2102, 2117, 4082
BaeGI GKGCMC 3 cut(s) 992, 2479, 2634
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 2597
BauI CACGAG 2 cut(s) 1523, 3557
BbsI GAAGAC 2 cut(s) 1248, 2955
Bbv12I GWGCWC 2 cut(s) 2634, 3075
BbvCI CCTCAGC 1 cut(s) 3411
BbvI GCAGC 6 cut(s) 361, 827, 2263, 2944, 2981, 3955
BciT130I CCWGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
BciVI GTATCC 3 cut(s) 382, 3319, 4382
BclI TGATCA 1 cut(s) 3598
BcoDI GTCTC 9 cut(s) 453, 979, 1321, 1527, 1875, 1908, 2292, 3292, 3308
BcuI ACTAGT 2 cut(s) 571, 2483
BfmI CTRYAG 3 cut(s) 618, 2967, 3224
BfoI RGCGCY 2 cut(s) 1491, 3411
BfuI GTATCC 3 cut(s) 382, 3319, 4382
BglII AGATCT 4 cut(s) 1567, 1745, 3514, 3818
BisI GCNGC 8 cut(s) 350, 587, 816, 1500, 2277, 2958, 2970, 3969
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 8 cut(s) 351, 588, 817, 1501, 2278, 2959, 2971, 3970
BmcAI AGTACT 2 cut(s) 423, 2327
Bme1390I CCNGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
Bme18I GGWCC 8 cut(s) 23, 761, 1347, 1574, 1730, 2102, 2117, 4082
BmeT110I CYCGRG 1 cut(s) 1530
BmrFI CCNGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
BmrI ACTGGG 2 cut(s) 1098, 2053
BmsI GCATC 9 cut(s) 1244, 1372, 1633, 1762, 1946, 1999, 3742, 3743, 4148
BmuI ACTGGG 2 cut(s) 1098, 2053
BpiI GAAGAC 2 cut(s) 1248, 2955
BplI GAGNNNNNCTC 4 cut(s) 1056, 1088, 1301, 1333
BpmI CTGGAG 4 cut(s) 2181, 2337, 3089, 3543
Bpu10I CCTNAGC 1 cut(s) 3411
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 3 cut(s) 1710, 3401, 3643
BsaAI YACGTR 1 cut(s) 2585
BsaBI GATNNNNATC 2 cut(s) 1744, 3440
BsaHI GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
BsaI GGTCTC 2 cut(s) 1527, 1908
BsaWI WCCGGW 1 cut(s) 1476
BsaXI ACNNNNNCTCC 6 cut(s) 388, 418, 3836, 3866, 4290, 4320
Bse118I RCCGGY 1 cut(s) 3755
Bse8I GATNNNNATC 2 cut(s) 1744, 3440
BseBI CCWGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
BseJI GATNNNNATC 2 cut(s) 1744, 3440
BseMII CTCAG 7 cut(s) 929, 1346, 1833, 1916, 1979, 3402, 4049
BseRI GAGGAG 4 cut(s) 213, 1456, 3326, 3540
BseSI GKGCMC 3 cut(s) 992, 2479, 2634
BseXI GCAGC 6 cut(s) 361, 827, 2263, 2944, 2981, 3955
BseYI CCCAGC 1 cut(s) 2279
BsgI GTGCAG 3 cut(s) 38, 459, 3635
Bsh1285I CGRYCG 1 cut(s) 1481
BshFI GGCC 7 cut(s) 43, 140, 186, 1484, 2803, 3259, 4019
BshNI GGYRCC 1 cut(s) 2597
BsiEI CGRYCG 1 cut(s) 1481
BsiHKAI GWGCWC 2 cut(s) 2634, 3075
BsiHKCI CYCGRG 1 cut(s) 1530
BsiSI CCGG 3 cut(s) 1470, 1477, 3756
BsmAI GTCTC 9 cut(s) 453, 979, 1321, 1527, 1875, 1908, 2292, 3292, 3308
BsmBI CGTCTC 1 cut(s) 979
BsmI GAATGC 2 cut(s) 711, 3278
BsnI GGCC 7 cut(s) 43, 140, 186, 1484, 2803, 3259, 4019
Bso31I GGTCTC 2 cut(s) 1527, 1908
BsoBI CYCGRG 1 cut(s) 1530
Bsp1286I GDGCHC 4 cut(s) 992, 2479, 2634, 3075
Bsp1720I GCTNAGC 1 cut(s) 675
Bsp19I CCATGG 3 cut(s) 35, 2106, 2253
BspANI GGCC 7 cut(s) 43, 140, 186, 1484, 2803, 3259, 4019
BspCNI CTCAG 7 cut(s) 928, 1347, 1834, 1917, 1978, 3403, 4050
BspHI TCATGA 3 cut(s) 838, 3031, 3679
BspMAI CTGCAG 3 cut(s) 622, 2971, 3228
BspQI GCTCTTC 1 cut(s) 3181
BspT107I GGYRCC 1 cut(s) 2597
BspTNI GGTCTC 2 cut(s) 1527, 1908
BsrBI CCGCTC 1 cut(s) 1935
BsrFI RCCGGY 1 cut(s) 3755
BssAI RCCGGY 1 cut(s) 3755
BssNI GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
BssSI CACGAG 2 cut(s) 1523, 3557
BssT1I CCWWGG 4 cut(s) 35, 2071, 2106, 2253
Bst2BI CACGAG 2 cut(s) 1523, 3557
Bst2UI CCWGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
Bst6I CTCTTC 4 cut(s) 106, 1810, 3181, 3643
BstACI GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
BstBAI YACGTR 1 cut(s) 2585
BstC8I GCNNGC 7 cut(s) 214, 622, 936, 1486, 1552, 2180, 3406
BstDSI CCRYGG 4 cut(s) 35, 2106, 2253, 2831
BstENI CCTNNNNNAGG 2 cut(s) 4076, 4210
BstH2I RGCGCY 2 cut(s) 1491, 3411
BstHHI GCGC 2 cut(s) 1490, 3410
BstMAI GTCTC 9 cut(s) 453, 979, 1321, 1527, 1875, 1908, 2292, 3292, 3308
BstMCI CGRYCG 1 cut(s) 1481
BstMWI GCNNNNNNNGC 8 cut(s) 222, 812, 1496, 2175, 2249, 2966, 2978, 3005
BstNI CCWGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
BstNSI RCATGY 4 cut(s) 1554, 1595, 1779, 4252
BstSCI CCNGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
BstSFI CTRYAG 3 cut(s) 618, 2967, 3224
BstSLI GKGCMC 3 cut(s) 992, 2479, 2634
BstV1I GCAGC 6 cut(s) 361, 827, 2263, 2944, 2981, 3955
BstV2I GAAGAC 2 cut(s) 1248, 2955
BstX2I RGATCY 8 cut(s) 746, 1567, 1701, 1745, 2591, 3514, 3637, 3818
BstXI CCANNNNNNTGG 2 cut(s) 957, 2128
BstYI RGATCY 8 cut(s) 746, 1567, 1701, 1745, 2591, 3514, 3637, 3818
BsuI GTATCC 3 cut(s) 382, 3319, 4382
BsuRI GGCC 7 cut(s) 43, 140, 186, 1484, 2803, 3259, 4019
BtgI CCRYGG 4 cut(s) 35, 2106, 2253, 2831
BtgZI GCGATG 1 cut(s) 4251
BtsI GCAGTG 1 cut(s) 3233
BtsIMutI CAGTG 7 cut(s) 304, 1663, 2041, 2361, 3171, 3233, 4097
Cac8I GCNNGC 7 cut(s) 214, 622, 936, 1486, 1552, 2180, 3406
CaiI CAGNNNCTG 1 cut(s) 4206
CciI TCATGA 3 cut(s) 838, 3031, 3679
CfoI GCGC 2 cut(s) 1490, 3410
Cfr10I RCCGGY 1 cut(s) 3755
CseI GACGC 4 cut(s) 1082, 1462, 2949, 3819
CsiI ACCWGGT 1 cut(s) 1536
Csp6I GTAC 9 cut(s) 405, 422, 1292, 2326, 2828, 3107, 3182, 3480, 4088
CviQI GTAC 9 cut(s) 405, 422, 1292, 2326, 2828, 3107, 3182, 3480, 4088
DraI TTTAAA 3 cut(s) 558, 3054, 3115
DrdI GACNNNNNNGTC 2 cut(s) 30, 1313
DseDI GACNNNNNNGTC 2 cut(s) 30, 1313
EaeI YGGCCR 2 cut(s) 184, 1482
Eam1104I CTCTTC 4 cut(s) 106, 1810, 3181, 3643
EarI CTCTTC 4 cut(s) 106, 1810, 3181, 3643
EciI GGCGGA 1 cut(s) 3589
Eco130I CCWWGG 4 cut(s) 35, 2071, 2106, 2253
Eco31I GGTCTC 2 cut(s) 1527, 1908
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 8 cut(s) 23, 761, 1347, 1574, 1730, 2102, 2117, 4082
Eco47III AGCGCT 1 cut(s) 3409
Eco57I CTGAAG 5 cut(s) 1097, 1755, 3162, 3882, 4191
Eco88I CYCGRG 1 cut(s) 1530
EcoNI CCTNNNNNAGG 2 cut(s) 4076, 4210
EcoO109I RGGNCCY 4 cut(s) 23, 41, 1347, 1730
EcoRI GAATTC 1 cut(s) 2617
EcoRII CCWGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
EcoRV GATATC 1 cut(s) 874
EcoT14I CCWWGG 4 cut(s) 35, 2071, 2106, 2253
EcoT22I ATGCAT 2 cut(s) 1026, 3278
ErhI CCWWGG 4 cut(s) 35, 2071, 2106, 2253
Esp3I CGTCTC 1 cut(s) 979
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 3652, 3684
FauI CCCGC 2 cut(s) 1527, 2187
FauNDI CATATG 2 cut(s) 1221, 2681
FbaI TGATCA 1 cut(s) 3598
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 8 cut(s) 350, 587, 816, 1500, 2277, 2958, 2970, 3969
Fsp4HI GCNGC 8 cut(s) 350, 587, 816, 1500, 2277, 2958, 2970, 3969
GlaI GCGC 2 cut(s) 1489, 3409
GluI GCNGC 8 cut(s) 350, 587, 816, 1500, 2277, 2958, 2970, 3969
GsaI CCCAGC 1 cut(s) 2283
GsuI CTGGAG 4 cut(s) 2181, 2337, 3089, 3543
HaeII RGCGCY 2 cut(s) 1491, 3411
HaeIII GGCC 7 cut(s) 43, 140, 186, 1484, 2803, 3259, 4019
HapII CCGG 3 cut(s) 1470, 1477, 3756
HgaI GACGC 4 cut(s) 1082, 1462, 2949, 3819
HhaI GCGC 2 cut(s) 1490, 3410
Hin1I GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
Hin6I GCGC 2 cut(s) 1488, 3408
HinP1I GCGC 2 cut(s) 1488, 3408
HindIII AAGCTT 4 cut(s) 210, 223, 273, 2979
HpaII CCGG 3 cut(s) 1470, 1477, 3756
Hpy166II GTNNAC 7 cut(s) 122, 2632, 2841, 3085, 3556, 4189, 4315
Hpy8I GTNNAC 7 cut(s) 122, 2632, 2841, 3085, 3556, 4189, 4315
Hpy99I CGWCG 1 cut(s) 1499
HpyCH4IV ACGT 5 cut(s) 972, 1161, 1267, 1675, 2584
HpyF10VI GCNNNNNNNGC 8 cut(s) 222, 812, 1496, 2175, 2249, 2966, 2978, 3005
HpySE526I ACGT 5 cut(s) 972, 1161, 1267, 1675, 2584
Hsp92I GRCGYC 4 cut(s) 1093, 1161, 1473, 3830
HspAI GCGC 2 cut(s) 1488, 3408
Ksp22I TGATCA 1 cut(s) 3598
LguI GCTCTTC 1 cut(s) 3181
LmnI GCTCC 8 cut(s) 396, 1012, 1078, 1168, 2649, 3070, 3488, 3857
Lsp1109I GCAGC 6 cut(s) 361, 827, 2263, 2944, 2981, 3955
LweI GCATC 9 cut(s) 1244, 1372, 1633, 1762, 1946, 1999, 3742, 3743, 4148
MabI ACCWGGT 1 cut(s) 1536
MaeII ACGT 5 cut(s) 972, 1161, 1267, 1675, 2584
MbiI CCGCTC 1 cut(s) 1935
MfeI CAATTG 2 cut(s) 2712, 3492
MflI RGATCY 8 cut(s) 746, 1567, 1701, 1745, 2591, 3514, 3637, 3818
MhlI GDGCHC 4 cut(s) 992, 2479, 2634, 3075
MlsI TGGCCA 1 cut(s) 186
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 9 cut(s) 955, 1074, 1288, 1535, 2076, 2515, 3374, 3946, 4088
MmeI TCCRAC 3 cut(s) 2166, 2371, 3605
Mox20I TGGCCA 1 cut(s) 186
Mph1103I ATGCAT 2 cut(s) 1026, 3278
MroXI GAANNNNTTC 4 cut(s) 2030, 2980, 3546, 3696
MscI TGGCCA 1 cut(s) 186
MslI CAYNNNNRTG 5 cut(s) 1211, 1240, 1911, 2796, 4320
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 2 cut(s) 1514, 2666
MspI CCGG 3 cut(s) 1470, 1477, 3756
MspR9I CCNGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
MssI GTTTAAAC 1 cut(s) 3115
MunI CAATTG 2 cut(s) 2712, 3492
Mva1269I GAATGC 2 cut(s) 711, 3278
MvaI CCWGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
MwoI GCNNNNNNNGC 8 cut(s) 222, 812, 1496, 2175, 2249, 2966, 2978, 3005
NcoI CCATGG 3 cut(s) 35, 2106, 2253
NdeI CATATG 2 cut(s) 1221, 2681
NmuCI GTSAC 8 cut(s) 923, 1190, 1304, 1687, 2014, 3629, 3722, 3831
NsiI ATGCAT 2 cut(s) 1026, 3278
NspI RCATGY 4 cut(s) 1554, 1595, 1779, 4252
PaeI GCATGC 1 cut(s) 1554
PagI TCATGA 3 cut(s) 838, 3031, 3679
PasI CCCWGGG 1 cut(s) 3484
PciI ACATGT 1 cut(s) 1591
PciSI GCTCTTC 1 cut(s) 3181
PctI GAATGC 2 cut(s) 711, 3278
PdmI GAANNNNTTC 4 cut(s) 2030, 2980, 3546, 3696
PflMI CCANNNNNTGG 3 cut(s) 2077, 2228, 3131
PkrI GCNGC 8 cut(s) 351, 588, 817, 1501, 2278, 2959, 2971, 3970
PleI GAGTC 9 cut(s) 955, 1073, 1287, 1534, 2075, 2515, 3373, 3945, 4088
PmeI GTTTAAAC 1 cut(s) 3115
PpsI GAGTC 9 cut(s) 955, 1073, 1287, 1534, 2075, 2515, 3373, 3945, 4088
Ppu21I YACGTR 1 cut(s) 2585
PpuMI RGGWCCY 3 cut(s) 23, 1347, 1730
PscI ACATGT 1 cut(s) 1591
PshBI ATTAAT 1 cut(s) 4349
PsiI TTATAA 1 cut(s) 1085
Psp5II RGGWCCY 3 cut(s) 23, 1347, 1730
Psp6I CCWGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
PspFI CCCAGC 1 cut(s) 2279
PspGI CCWGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
PspPPI RGGWCCY 3 cut(s) 23, 1347, 1730
PstI CTGCAG 3 cut(s) 622, 2971, 3228
PstNI CAGNNNCTG 1 cut(s) 4206
PsuI RGATCY 8 cut(s) 746, 1567, 1701, 1745, 2591, 3514, 3637, 3818
PvuII CAGCTG 1 cut(s) 2666
RsaI GTAC 9 cut(s) 406, 423, 1293, 2327, 2829, 3108, 3183, 3481, 4089
RsaNI GTAC 9 cut(s) 405, 422, 1292, 2326, 2828, 3107, 3182, 3480, 4088
RseI CAYNNNNRTG 5 cut(s) 1211, 1240, 1911, 2796, 4320
SapI GCTCTTC 1 cut(s) 3181
SatI GCNGC 8 cut(s) 350, 587, 816, 1500, 2277, 2958, 2970, 3969
ScaI AGTACT 2 cut(s) 423, 2327
SchI GAGTC 9 cut(s) 955, 1074, 1288, 1535, 2076, 2515, 3374, 3946, 4088
ScrFI CCNGG 8 cut(s) 45, 1538, 2122, 2199, 2452, 3144, 3171, 3485
SduI GDGCHC 4 cut(s) 992, 2479, 2634, 3075
SexAI ACCWGGT 1 cut(s) 1536
SfaNI GCATC 9 cut(s) 1244, 1372, 1633, 1762, 1946, 1999, 3742, 3743, 4148
SfcI CTRYAG 3 cut(s) 618, 2967, 3224
SinI GGWCC 8 cut(s) 23, 761, 1347, 1574, 1730, 2102, 2117, 4082
SmiMI CAYNNNNRTG 5 cut(s) 1211, 1240, 1911, 2796, 4320
SmlI CTYRAG 3 cut(s) 1725, 3380, 3658
SmoI CTYRAG 3 cut(s) 1725, 3380, 3658
SpeI ACTAGT 2 cut(s) 571, 2483
SphI GCATGC 1 cut(s) 1554
SspI AATATT 7 cut(s) 889, 1016, 2092, 2557, 3868, 4291, 4404
StyD4I CCNGG 8 cut(s) 43, 1536, 2120, 2197, 2450, 3142, 3169, 3483
StyI CCWWGG 4 cut(s) 35, 2071, 2106, 2253
TaiI ACGT 5 cut(s) 975, 1164, 1270, 1678, 2587
TaqI TCGA 4 cut(s) 1937, 2540, 2723, 3564
TatI WGTACW 5 cut(s) 404, 421, 2325, 3181, 4087
TauI GCSGC 2 cut(s) 589, 1502
TscAI CASTG 7 cut(s) 311, 1663, 2041, 2361, 3171, 3233, 4104
TseFI GTSAC 8 cut(s) 923, 1190, 1304, 1687, 2014, 3629, 3722, 3831
TseI GCWGC 6 cut(s) 349, 815, 2276, 2957, 2969, 3968
Tsp45I GTSAC 8 cut(s) 923, 1190, 1304, 1687, 2014, 3629, 3722, 3831
TspGWI ACGGA 2 cut(s) 1483, 2811
TspRI CASTG 7 cut(s) 311, 1663, 2041, 2361, 3171, 3233, 4104
Van91I CCANNNNNTGG 3 cut(s) 2077, 2228, 3131
VneI GTGCAC 1 cut(s) 2630
VpaK11BI GGWCC 8 cut(s) 23, 761, 1347, 1574, 1730, 2102, 2117, 4082
VspI ATTAAT 1 cut(s) 4349
XagI CCTNNNNNAGG 2 cut(s) 4076, 4210
XapI RAATTY 6 cut(s) 1436, 2365, 2617, 3502, 3695, 4327
XbaI TCTAGA 1 cut(s) 2020
XceI RCATGY 4 cut(s) 1554, 1595, 1779, 4252
XcmI CCANNNNNNNNNTGG 2 cut(s) 2078, 3788
XmiI GTMKAC 1 cut(s) 121
XmnI GAANNNNTTC 4 cut(s) 2030, 2980, 3546, 3696
ZraI GACGTC 1 cut(s) 1162
ZrmI AGTACT 2 cut(s) 423, 2327
Zsp2I ATGCAT 2 cut(s) 1026, 3278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.