MD12G1226700.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007241
Physical Location & Seq
Forward (+)
30241042 .. 30247765
6724 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1226700.v1.1.491

Sequence Viewer

Length: 2949 bp
ATGAAGAAGAACCGAAACGGCGGCACCAAGGTTGTTCAGAGTAATCATCATCAACACGCCAATAACAATGGCTTCCTTCCGAATTCTCTCAAATTCATTTCTTCTTGCATTAAAACTGCATCTTCCGGCGTAAGGTCGGGCGCCGCCTCCGTCGCAGCCTCCATCTCCGTCGATTCTCACGATTGCCGAGACCAGGTGTTGTGGGCTTGCTTCGACAAAGTAGAGCTTGGTCCATCTTCCTTCAAACATGTTCTGCTACTTGGTTATTCCAATGGATTTCAAGTCCTTGATGTTGAAGATGCCTCTAATTTCAGTGAACTTGTCTCAAGGCATGATGATCCAGTTACATTTTTACAGATGCAGCCCCTGCCTGCAAAGTGTGAGGGTCATGAAGGATTCAGATCCTCACATCCTATACTCATGGTTGTTGCATGTGATGAAAAGAGCTCAGGTATGATGCAAACAGGGAGAGATGGGTTGGCCAATGGTCATTCTGAGCCCCAAACAGGAAACTCTGCTTTGTCTCCTACAGCTGTTCGGTTTTATTCGCTAAGGTCTTGTAGTTATGTTCATGTTCTAAGATTCCGTTCAACTGTGTATATGGTTAGATGCAGTCCTCAAGTAGTAGCTGTGGGTCTTGCATCACAAATATACTGCTTCGATGCTGTCACCCTTGAGAACAAATTCAGTGTCCTTACTTATCCTGTCCCCCAGTTGGGAGTTCAAGGACTGGTTGGGGTCAATATCGGGTATGGTCCCATGGCTGTTGGTCCCAGGTGGTTGGCTTATGCTTCCAACAACCCTCTGATGTCAAACACAGGCCGCCTCAGTCCTCAAAGTCTTACTCCACCTGGTGTAAGTCCATCAACTTCGCCAAGTAGTGGAAGTCTGATGGCTCGATATGCAATGGAATCTAGTAAACAGTTAGCTTCCGGGTTACTTAATCTTGGAGACATGGGTTACAAAACTTTGTCCAAGTACTATCAAGATCTAACACCAGATGGTTCTAGCTCTCCTGTGTCATCAAATTCTAGCTGGAAAGTTGGGAGGGTTGTATCACATTCAACCGAGACAGATATTGCTGGAATTGTTGTTATAAAAGACTTTGTTTCCAGAGCTGTTGTGTCACAATTCAGGGCACATACCAGTCCCATTTCTGCCCTGTGTTTTGACCCAAGTGGGACTCTTCTAGTTACTGCCTCAATACATGGAAACAATATAAACATTTTTCGAATAATGCCATCCTGCTCGCGTAATGGATCATGCACTCAGGGCTTTGACTGGACTTCTTCGCATGTGCACCTTTACAAACTACATCGTGGCATAACCTCTGCTGTGATCCAAGACATTTGTTTTAGTCCATACAGTCAATGGATTACAATTGTTACATCCAGGGGAACTAGCCATATTTTTGCTTTATCTCCCTTCGGTGGTGACACTGTTTTGCAAATACAAAATTCCCATGTTGACAGACCTACTCTTTCAACGGTTCCATCTGCACCATGGTGGTTTACTCCATATTTCACAAGAAGTCAGCAAACCTTTTCTCCACCTCCGGCAGCTACCCTTTCTGTGGTTAGCAGAATAAAAAATAACTCCGGGTGGCTCAACACTGTTAGTAATGCAGCATCTTCTGCAGCAGGAAAAGGTTCCTTTCGATCTGGGGCTGTTGCCGCTGTATTCCATAGTTCTGTACCTGATGGTCTGCAATCTTTCCATGCAAAGGTCACTGCATTGGAGCACTTATTGGCTTATACTGCTTCTGGTCATGTAATTCAGTATAAACTACTGCCTTCATTGGGAGGAGAGGCAGGCGATTCTGTTTCAGGAGCAGGGCCAGGTTCTTCAGTTCAGATACAGGATGAAGATTTGCGAGTGAAAGTTGAATCTCTTCATTTCTTGGATGTTTGCCGAAGAAATGATTGGCCTGAAAGAGAGGAAGGCATTTCTGGGATTATACTTGAGAAACAAGACGATATTGACAGAATTGATAGTTCTGACTGTGATGACAATGATGTTGAGGATAAGGAGTTGGCAAAACCCCTTGAACGATCTCACATGTACCTCTCTAATGCTGAGGTTCAGATCAATTCTGGAAGGATTCCAATCTGGCAAAAGTCTAAGATATATTTCTATACAATGAGTCCGTCTGCTGCTGGTGAGCTAAATTTTGGTAAAGATCTTACCGGCGGAGAGATGGAAATAGAGAAGGTTCCTGTTCACGAGGTTGAAATGAGGCGGAAATATTTGCTACCTGTTGTTCATCCTTTTCATAGGTTTCAGTCTGACTGGAATGGCAGGCATCTAATTGGAGGAGGATGCTCGAGTTCATCTTCTGATTCTCACAAAGATAAAGAAAAGTTTCAGGAAAATTCTGGTAGTTCTGGTGAGAATTTGACACCTATAGGCTCAGCTGAAAACCCAAATGCTGGTGATTATCCATTTATTCTCCAACCTGGAAATGGTCAGAATGGCGAAAAAAGAGGGAGATCATTTTCGGTATCACCTCACATGAACTATATCTCCACAAAGAAAAATATTACCTCAGTATCCCTGAAACAACCTACCTCAGGTGTTTCTCTTGGTGACGATAGTAATTTTTCAAATAGTCTATCCACTTTAACAAGCAGCTCCCTTTCAGCTGATAGAACTATTGCAACAAAGGTTCAGTCGGTGAATAGTGGTGGGGCCAGTGAAGGTTCCAATGTAGGTTCTAACCGTTCTGATCTGAGTATGAACATGCTCGATGAGGGACCAGTACACAAATCACCGGATTTTGAGCCATTTTTCCAAGAGGGGTATTGCAAAGCATCACCTCTGAGCAACTTTCGTGAGTCGACTGAAGTTGTAACTGATGTTGACAGCCCCTGTGATAGGGAGAAAAGCGAGGAAGATGATGACAATGACGACATGCTTGGTGGTGTATTTGCCTTCTCCGAGGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

983

Amino Acids

106.12

Weight (kDa)

5.99

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 84 - 485 8.4e-37 BCAS3, WD40 repeat
BCAS3 PF12490 617 - 753 8.5e-38 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1097
AccB1I GGYRCC 2 cut(s) 23, 140
AccB7I CCANNNNNTGG 2 cut(s) 881, 2429
AccI GTMKAC 1 cut(s) 2837
AccII CGCG 1 cut(s) 1252
AciI CCGC 6 cut(s) 21, 144, 823, 1674, 2190, 2239
AclWI GGATC 4 cut(s) 332, 396, 1267, 1333
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 8 cut(s) 82, 92, 683, 1027, 1456, 2167, 2371, 2392
AcuI CTGAAG 2 cut(s) 1830, 2862
AcyI GRCGYC 1 cut(s) 141
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 4 cut(s) 980, 1695, 2063, 2760
AflIII ACRYGT 2 cut(s) 247, 2058
AjnI CCWGG 6 cut(s) 192, 773, 850, 1391, 1837, 2455
AjuI GAANNNNNNNTTGG 2 cut(s) 1906, 1938
AleI CACNNNNGTG 1 cut(s) 1504
AloI GAACNNNNNNTCC 2 cut(s) 2507, 2539
Alw21I GWGCWC 3 cut(s) 449, 1302, 1743
Alw26I GTCTC 5 cut(s) 183, 328, 528, 945, 1064
Alw44I GTGCAC 1 cut(s) 1298
AlwI GGATC 4 cut(s) 332, 396, 1267, 1333
AlwNI CAGNNNCTG 2 cut(s) 367, 2868
Ama87I CYCGRG 1 cut(s) 2323
AoxI GGCC 5 cut(s) 480, 820, 1835, 1925, 2688
ApaLI GTGCAC 1 cut(s) 1298
ApeKI GCWGC 7 cut(s) 155, 361, 1559, 1625, 1637, 2153, 2628
ApoI RAATTY 8 cut(s) 82, 92, 683, 1027, 1456, 2167, 2371, 2392
ArsI GACNNNNNNTTYG 4 cut(s) 675, 707, 2654, 2686
Asp700I GAANNNNTTC 4 cut(s) 683, 1648, 1890, 2361
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 6 cut(s) 230, 755, 770, 1835, 2688, 2753
AsuC2I CCSGG 2 cut(s) 934, 1600
AsuII TTCGAA 1 cut(s) 1231
AvaI CYCGRG 1 cut(s) 2323
AvaII GGWCC 4 cut(s) 230, 755, 770, 2753
AxyI CCTNAGG 1 cut(s) 2569
BaeGI GKGCMC 2 cut(s) 1141, 1302
BaeI ACNNNNGTAYC 2 cut(s) 2532, 2565
BalI TGGCCA 1 cut(s) 482
BanI GGYRCC 2 cut(s) 23, 140
BanII GRGCYC 2 cut(s) 449, 501
BauI CACGAG 1 cut(s) 2222
Bbv12I GWGCWC 3 cut(s) 449, 1302, 1743
BbvCI CCTCAGC 1 cut(s) 2076
BbvI GCAGC 7 cut(s) 167, 373, 1571, 1637, 1649, 2140, 2640
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 6 cut(s) 194, 775, 852, 1393, 1839, 2457
BciVI GTATCC 1 cut(s) 2560
BcnI CCSGG 2 cut(s) 934, 1600
BcoDI GTCTC 5 cut(s) 183, 328, 528, 945, 1064
BfaI CTAG 5 cut(s) 915, 1008, 1032, 1190, 1401
BfmI CTRYAG 3 cut(s) 528, 1635, 2403
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 1 cut(s) 2560
BglII AGATCT 2 cut(s) 988, 2179
BlpI GCTNAGC 1 cut(s) 2410
BmcAI AGTACT 1 cut(s) 980
Bme1390I CCNGG 8 cut(s) 194, 775, 852, 934, 1393, 1600, 1839, 2457
Bme18I GGWCC 4 cut(s) 230, 755, 770, 2753
BmeT110I CYCGRG 1 cut(s) 2323
BmgT120I GGNCC 6 cut(s) 230, 755, 770, 1835, 2688, 2753
BmrFI CCNGG 8 cut(s) 194, 775, 852, 934, 1393, 1600, 1839, 2457
BmrI ACTGGG 1 cut(s) 706
BmuI ACTGGG 1 cut(s) 706
Bpu10I CCTNAGC 3 cut(s) 448, 551, 2076
Bpu1102I GCTNAGC 1 cut(s) 2410
Bpu14I TTCGAA 1 cut(s) 1231
BpuEI CTTGAG 4 cut(s) 310, 603, 695, 1982
BpuMI CCSGG 2 cut(s) 934, 1600
BsaBI GATNNNNATC 2 cut(s) 400, 2105
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 6 cut(s) 27, 759, 773, 1392, 1502, 2937
BsaWI WCCGGW 1 cut(s) 2770
BsaXI ACNNNNNCTCC 4 cut(s) 1531, 1561, 2507, 2537
Bse118I RCCGGY 1 cut(s) 2186
Bse1I ACTGG 8 cut(s) 341, 712, 735, 1146, 1286, 2294, 2691, 2756
Bse21I CCTNAGG 1 cut(s) 2569
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 2 cut(s) 400, 2105
BseBI CCWGG 6 cut(s) 194, 775, 852, 1393, 1839, 2457
BseDI CCNNGG 6 cut(s) 27, 759, 773, 1392, 1502, 2937
BseGI GGATG 7 cut(s) 409, 1241, 1388, 1867, 1909, 2263, 2324
BseJI GATNNNNATC 2 cut(s) 400, 2105
BseMI GCAATG 1 cut(s) 912
BseNI ACTGG 8 cut(s) 341, 712, 735, 1146, 1286, 2294, 2691, 2756
BseRI GAGGAG 2 cut(s) 1818, 2328
BseSI GKGCMC 2 cut(s) 1141, 1302
BseXI GCAGC 7 cut(s) 167, 373, 1571, 1637, 1649, 2140, 2640
BsgI GTGCAG 1 cut(s) 1482
Bsh1236I CGCG 1 cut(s) 1252
BshFI GGCC 5 cut(s) 482, 822, 1837, 1927, 2690
BshNI GGYRCC 2 cut(s) 23, 140
BsiHKAI GWGCWC 3 cut(s) 449, 1302, 1743
BsiHKCI CYCGRG 1 cut(s) 2323
BsiSI CCGG 6 cut(s) 126, 933, 1556, 1599, 2187, 2771
BslFI GGGAC 6 cut(s) 692, 741, 756, 1134, 1195, 2766
BsmAI GTCTC 5 cut(s) 183, 328, 528, 945, 1064
BsmFI GGGAC 6 cut(s) 692, 741, 756, 1134, 1195, 2766
BsnI GGCC 5 cut(s) 482, 822, 1837, 1927, 2690
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 2323
Bsp119I TTCGAA 1 cut(s) 1231
Bsp1286I GDGCHC 5 cut(s) 449, 501, 1141, 1302, 1743
Bsp1720I GCTNAGC 1 cut(s) 2410
Bsp19I CCATGG 2 cut(s) 759, 1502
BspACI CCGC 6 cut(s) 21, 144, 823, 1674, 2190, 2239
BspANI GGCC 5 cut(s) 482, 822, 1837, 1927, 2690
BspFNI CGCG 1 cut(s) 1252
BspHI TCATGA 1 cut(s) 388
BspMAI CTGCAG 1 cut(s) 1639
BspPI GGATC 4 cut(s) 332, 396, 1267, 1333
BspT104I TTCGAA 1 cut(s) 1231
BspT107I GGYRCC 2 cut(s) 23, 140
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 912
BsrFI RCCGGY 1 cut(s) 2186
BsrI ACTGG 8 cut(s) 341, 712, 735, 1146, 1286, 2294, 2691, 2756
BssAI RCCGGY 1 cut(s) 2186
BssECI CCNNGG 6 cut(s) 27, 759, 773, 1392, 1502, 2937
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 2222
BssT1I CCWWGG 3 cut(s) 27, 759, 1502
Bst2BI CACGAG 1 cut(s) 2222
Bst2UI CCWGG 6 cut(s) 194, 775, 852, 1393, 1839, 2457
Bst4CI ACNGT 8 cut(s) 595, 924, 1367, 1441, 1489, 1615, 2003, 2720
Bst6I CTCTTC 2 cut(s) 1191, 1896
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 2 cut(s) 367, 1634
BstBI TTCGAA 1 cut(s) 1231
BstC8I GCNNGC 5 cut(s) 208, 372, 1250, 1813, 2300
BstDSI CCRYGG 2 cut(s) 759, 1502
BstENI CCTNNNNNAGG 2 cut(s) 2568, 2872
BstF5I GGATG 7 cut(s) 409, 1241, 1388, 1867, 1909, 2263, 2324
BstFNI CGCG 1 cut(s) 1252
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstMAI GTCTC 5 cut(s) 183, 328, 528, 945, 1064
BstMWI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1272, 1634, 1673, 1757
BstNI CCWGG 6 cut(s) 194, 775, 852, 1393, 1839, 2457
BstNSI RCATGY 6 cut(s) 251, 435, 1298, 2062, 2743, 2914
BstSCI CCNGG 8 cut(s) 192, 773, 850, 932, 1391, 1598, 1837, 2455
BstSFI CTRYAG 3 cut(s) 528, 1635, 2403
BstSLI GKGCMC 2 cut(s) 1141, 1302
BstUI CGCG 1 cut(s) 1252
BstV1I GCAGC 7 cut(s) 167, 373, 1571, 1637, 1649, 2140, 2640
BstX2I RGATCY 3 cut(s) 401, 988, 2179
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 3 cut(s) 401, 988, 2179
Bsu36I CCTNAGG 1 cut(s) 2569
BsuI GTATCC 1 cut(s) 2560
BsuRI GGCC 5 cut(s) 482, 822, 1837, 1927, 2690
BtgI CCRYGG 2 cut(s) 759, 1502
BtsCI GGATG 7 cut(s) 409, 1241, 1388, 1867, 1909, 2263, 2324
BtsI GCAGTG 1 cut(s) 1728
BtsIMutI CAGTG 6 cut(s) 319, 694, 1437, 1611, 1728, 2698
Cac8I GCNNGC 5 cut(s) 208, 372, 1250, 1813, 2300
CaiI CAGNNNCTG 2 cut(s) 367, 2868
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 2186
Cfr13I GGNCC 6 cut(s) 230, 755, 770, 1835, 2688, 2753
CsiI ACCWGGT 2 cut(s) 192, 850
Csp6I GTAC 4 cut(s) 979, 1694, 2062, 2759
CviQI GTAC 4 cut(s) 979, 1694, 2062, 2759
DinI GGCGCC 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 1191, 1896
EarI CTCTTC 2 cut(s) 1191, 1896
EciI GGCGGA 2 cut(s) 2205, 2254
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 3 cut(s) 27, 759, 1502
Eco24I GRGCYC 2 cut(s) 449, 501
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 4 cut(s) 230, 755, 770, 2753
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 2 cut(s) 1830, 2862
Eco81I CCTNAGG 1 cut(s) 2569
Eco88I CYCGRG 1 cut(s) 2323
EcoICRI GAGCTC 1 cut(s) 447
EcoNI CCTNNNNNAGG 2 cut(s) 2568, 2872
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 6 cut(s) 192, 773, 850, 1391, 1837, 2455
EcoT14I CCWWGG 3 cut(s) 27, 759, 1502
EcoT38I GRGCYC 2 cut(s) 449, 501
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 3 cut(s) 27, 759, 1502
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 6 cut(s) 692, 741, 756, 1134, 1195, 2766
FblI GTMKAC 1 cut(s) 2837
FokI GGATG 7 cut(s) 396, 1228, 1375, 1874, 1916, 2250, 2331
FriOI GRGCYC 2 cut(s) 449, 501
FspBI CTAG 5 cut(s) 915, 1008, 1032, 1190, 1401
GlaI GCGC 1 cut(s) 142
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 5 cut(s) 482, 822, 1837, 1927, 2690
HapII CCGG 6 cut(s) 126, 933, 1556, 1599, 2187, 2771
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 141
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 3 cut(s) 1468, 2838, 2860
HindII GTYRAC 3 cut(s) 1468, 2838, 2860
HpaII CCGG 6 cut(s) 126, 933, 1556, 1599, 2187, 2771
Hpy166II GTNNAC 9 cut(s) 317, 920, 1300, 1468, 1512, 2221, 2761, 2838, 2860
Hpy188III TCNNGA 9 cut(s) 179, 389, 986, 1113, 1827, 2094, 2222, 2366, 2831
Hpy8I GTNNAC 9 cut(s) 317, 920, 1300, 1468, 1512, 2221, 2761, 2838, 2860
Hpy99I CGWCG 2 cut(s) 155, 173
HpyCH4III ACNGT 8 cut(s) 595, 924, 1367, 1441, 1489, 1615, 2003, 2720
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1272, 1634, 1673, 1757
Hsp92I GRCGYC 1 cut(s) 141
HspAI GCGC 1 cut(s) 141
KasI GGCGCC 1 cut(s) 140
LmnI GCTCC 3 cut(s) 1738, 1829, 2636
Lsp1109I GCAGC 7 cut(s) 167, 373, 1571, 1637, 1649, 2140, 2640
MabI ACCWGGT 2 cut(s) 192, 850
MaeI CTAG 5 cut(s) 915, 1008, 1032, 1190, 1401
MfeI CAATTG 1 cut(s) 1380
MflI RGATCY 3 cut(s) 401, 988, 2179
MhlI GDGCHC 5 cut(s) 449, 501, 1141, 1302, 1743
MlsI TGGCCA 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 482
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 3 cut(s) 1177, 2152, 2843
MmeI TCCRAC 2 cut(s) 819, 2476
Mox20I TGGCCA 1 cut(s) 482
MroXI GAANNNNTTC 4 cut(s) 683, 1648, 1890, 2361
MscI TGGCCA 1 cut(s) 482
MseI TTAA 3 cut(s) 111, 942, 2621
MslI CAYNNNNRTG 1 cut(s) 1504
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 4 cut(s) 533, 1676, 2414, 2642
MspI CCGG 6 cut(s) 126, 933, 1556, 1599, 2187, 2771
MspR9I CCNGG 8 cut(s) 194, 775, 852, 934, 1393, 1600, 1839, 2457
MunI CAATTG 1 cut(s) 1380
MvaI CCWGG 6 cut(s) 194, 775, 852, 1393, 1839, 2457
MvnI CGCG 1 cut(s) 1252
MwoI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1272, 1634, 1673, 1757
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 2 cut(s) 934, 1600
NcoI CCATGG 2 cut(s) 759, 1502
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 5 cut(s) 667, 1125, 1433, 1726, 2585
NspI RCATGY 6 cut(s) 251, 435, 1298, 2062, 2743, 2914
NspV TTCGAA 1 cut(s) 1231
OliI CACNNNNGTG 1 cut(s) 1504
PaeR7I CTCGAG 1 cut(s) 2323
PagI TCATGA 1 cut(s) 388
PciI ACATGT 2 cut(s) 247, 2058
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PdmI GAANNNNTTC 4 cut(s) 683, 1648, 1890, 2361
PfeI GAWTC 8 cut(s) 173, 396, 582, 911, 1817, 1886, 2101, 2339
PflMI CCANNNNNTGG 2 cut(s) 881, 2429
PleI GAGTC 3 cut(s) 1177, 2151, 2842
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 3 cut(s) 1177, 2151, 2842
PscI ACATGT 2 cut(s) 247, 2058
PsiI TTATAA 1 cut(s) 1097
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 6 cut(s) 192, 773, 850, 1391, 1837, 2455
PspGI CCWGG 6 cut(s) 192, 773, 850, 1391, 1837, 2455
PspPI GGNCC 6 cut(s) 230, 755, 770, 1835, 2688, 2753
PspXI VCTCGAGB 1 cut(s) 2323
PstI CTGCAG 1 cut(s) 1639
PstNI CAGNNNCTG 2 cut(s) 367, 2868
PsuI RGATCY 3 cut(s) 401, 988, 2179
PvuII CAGCTG 3 cut(s) 533, 2414, 2642
RsaI GTAC 4 cut(s) 980, 1695, 2063, 2760
RsaNI GTAC 4 cut(s) 979, 1694, 2062, 2759
RseI CAYNNNNRTG 1 cut(s) 1504
SacI GAGCTC 1 cut(s) 449
SalI GTCGAC 1 cut(s) 2836
SaqAI TTAA 3 cut(s) 111, 942, 2621
Sau96I GGNCC 6 cut(s) 230, 755, 770, 1835, 2688, 2753
ScaI AGTACT 1 cut(s) 980
SchI GAGTC 3 cut(s) 1177, 2152, 2843
ScrFI CCNGG 8 cut(s) 194, 775, 852, 934, 1393, 1600, 1839, 2457
SduI GDGCHC 5 cut(s) 449, 501, 1141, 1302, 1743
SexAI ACCWGGT 2 cut(s) 192, 850
SfcI CTRYAG 3 cut(s) 528, 1635, 2403
SfoI GGCGCC 1 cut(s) 142
Sfr274I CTCGAG 1 cut(s) 2323
SfuI TTCGAA 1 cut(s) 1231
SinI GGWCC 4 cut(s) 230, 755, 770, 2753
SlaI CTCGAG 1 cut(s) 2323
SmiMI CAYNNNNRTG 1 cut(s) 1504
SmlI CTYRAG 5 cut(s) 325, 618, 674, 1961, 2323
SmoI CTYRAG 5 cut(s) 325, 618, 674, 1961, 2323
SsiI CCGC 6 cut(s) 21, 144, 823, 1674, 2190, 2239
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 2 cut(s) 2246, 2539
SspMI CTAG 5 cut(s) 915, 1008, 1032, 1190, 1401
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 8 cut(s) 192, 773, 850, 932, 1391, 1598, 1837, 2455
StyI CCWWGG 3 cut(s) 27, 759, 1502
TaaI ACNGT 8 cut(s) 595, 924, 1367, 1441, 1489, 1615, 2003, 2720
TaqI TCGA 9 cut(s) 171, 213, 660, 898, 1231, 1657, 2324, 2745, 2837
TatI WGTACW 2 cut(s) 978, 2758
TauI GCSGC 4 cut(s) 24, 146, 825, 1676
TfiI GAWTC 8 cut(s) 173, 396, 582, 911, 1817, 1886, 2101, 2339
Tru1I TTAA 3 cut(s) 111, 942, 2621
Tru9I TTAA 3 cut(s) 111, 942, 2621
TscAI CASTG 6 cut(s) 319, 694, 1444, 1618, 1735, 2698
TseFI GTSAC 5 cut(s) 667, 1125, 1433, 1726, 2585
TseI GCWGC 7 cut(s) 155, 361, 1559, 1625, 1637, 2153, 2628
Tsp45I GTSAC 5 cut(s) 667, 1125, 1433, 1726, 2585
TspGWI ACGGA 4 cut(s) 139, 157, 575, 2136
TspRI CASTG 6 cut(s) 319, 694, 1444, 1618, 1735, 2698
Van91I CCANNNNNTGG 2 cut(s) 881, 2429
VneI GTGCAC 1 cut(s) 1298
VpaK11BI GGWCC 4 cut(s) 230, 755, 770, 2753
XagI CCTNNNNNAGG 2 cut(s) 2568, 2872
XapI RAATTY 8 cut(s) 82, 92, 683, 1027, 1456, 2167, 2371, 2392
XceI RCATGY 6 cut(s) 251, 435, 1298, 2062, 2743, 2914
XcmI CCANNNNNNNNNTGG 3 cut(s) 1368, 1500, 2459
XhoI CTCGAG 1 cut(s) 2323
XmiI GTMKAC 1 cut(s) 2837
XmnI GAANNNNTTC 4 cut(s) 683, 1648, 1890, 2361
XspI CTAG 5 cut(s) 915, 1008, 1032, 1190, 1401
ZrmI AGTACT 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.