pycom04g18800

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007241
Physical Location & Seq
Forward (+)
20688721 .. 20690229
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18800.1

Sequence Viewer

Length: 1059 bp
ATGGTTGTTATAAAAGACTTTGTTTCCAGAGCCATTGTATCACAGTTCAGGGCACATACTAGTCCCATTTCTGCCCTGTGTTTTGACCCAAGCGGGACTCTTCTAGTTACTGCTTCAATACATGGAAACAATATAAACATTTTTCGAATAATACCATCCTGCTCACGTAATGGATCAAGCACTCAAAGCTATGACTGGACTTCTTCTCATGTGCACCTTTACAAACTACATCGTGGCATAACATCTGCTGTGATCCAAGACATTTGTTTTAGTCAATACAGTCAATGGATCACAATTGTTTCATCCAGGGGGACTAGCCATATTTTTGCTTTGTCTCCATTCGGTGGTGAAACTGTTCTTCAAATACAGAATTCTCATGTTGACGGACCTACTCTTTCACCGGTTCCATCTGCACCATGGTGGTTTACTCCATATTTTACAAGAAGTCAGCAACTCTTTTCTCCACCTCCAGCAGCTACCCTTTCTGTAGTTAGCAGAATAAAAAATAACTGTGGGTGGCTCAACACTGTTAGTAATGCTGCATCTTCTGCAGCAGGAAAGGCTTCCTTTTGGTCTGGGGCTGTTGCCGCTGTATTCCATAGTTCTGTACCTCATGACATGCAATCTTCCCATGCGAAGGTCACTGCGTTGGAGCACCTATTGGCTTATACTGCTTCTGGTCATGTAATTCAGTATAAACTACTGCCTTCGTTGGGAGGAGAGCCAGGCAACGCTGCTTCGAGAGCAGGGCCAGGTTCTTCAGTACAGATACAGGATGAAGAATTGCGAGTGAAAGTTGAATCTCTTCATTTCTTGGATGTTTGCCGCAGAAATGATTGGCCTGAAAGAGAGGAATGCATTTCTGGGGTTATACTTGGGAAACAAGAAGATATTGATACAATTGATAGTTCTGACTGTGATGACAATGATGTTGGGGATAAGGAGTTGGCAAAACCCCTTGAACGATCGATCATGTGTATCTCTCCAATGCTGAGGTTCAGATCAATTCCGGAAGGATTCCAATCTGGCAAAAGTCTAAGATATATTTCTATACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

353

Amino Acids

38.42

Weight (kDa)

6.66

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 2 - 122 1.5e-22 BCAS3, WD40 repeat
BCAS3 PF12490 255 - 313 6.4e-08 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 1009
AciI CCGC 3 cut(s) 93, 588, 826
AclWI GGATC 3 cut(s) 181, 247, 296
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 2 cut(s) 609, 765
AfiI CCNNNNNNNGG 3 cut(s) 344, 637, 713
AgeI ACCGGT 1 cut(s) 400
AgsI TTSAA 4 cut(s) 117, 362, 800, 962
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 3 cut(s) 305, 724, 751
AleI CACNNNNGTG 1 cut(s) 418
AluBI AGCT 2 cut(s) 189, 476
AluI AGCT 2 cut(s) 189, 476
Alw21I GWGCWC 2 cut(s) 216, 657
Alw26I GTCTC 1 cut(s) 339
Alw44I GTGCAC 1 cut(s) 212
AlwI GGATC 3 cut(s) 181, 247, 296
Aor13HI TCCGGA 1 cut(s) 1009
AoxI GGCC 2 cut(s) 749, 839
ApaLI GTGCAC 1 cut(s) 212
ApeKI GCWGC 4 cut(s) 473, 539, 551, 734
ApoI RAATTY 1 cut(s) 370
AsiGI ACCGGT 1 cut(s) 400
Asp700I GAANNNNTTC 3 cut(s) 354, 562, 804
AspS9I GGNCC 2 cut(s) 386, 749
AsuHPI GGTGA 2 cut(s) 359, 390
AsuII TTCGAA 1 cut(s) 145
AvaII GGWCC 1 cut(s) 386
BaeGI GKGCMC 2 cut(s) 55, 216
Bbv12I GWGCWC 2 cut(s) 216, 657
BbvCI CCTCAGC 1 cut(s) 992
BbvI GCAGC 4 cut(s) 485, 526, 563, 721
BccI CCATC 2 cut(s) 163, 415
BciT130I CCWGG 3 cut(s) 307, 726, 753
BcoDI GTCTC 1 cut(s) 339
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 3 cut(s) 60, 104, 315
BfmI CTRYAG 2 cut(s) 486, 549
BisI GCNGC 6 cut(s) 474, 540, 552, 588, 735, 826
BlsI GCNGC 6 cut(s) 475, 541, 553, 589, 736, 827
Bme1390I CCNGG 3 cut(s) 307, 726, 753
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 386, 749
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 3 cut(s) 307, 726, 753
BmsI GCATC 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 1 cut(s) 992
Bpu14I TTCGAA 1 cut(s) 145
Bsa29I ATCGAT 1 cut(s) 968
BsaAI YACGTR 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 1021
BsaJI CCNNGG 2 cut(s) 306, 416
BsaWI WCCGGW 2 cut(s) 400, 1009
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 637, 713
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 200
Bse8I GATNNNNATC 1 cut(s) 1021
BseAI TCCGGA 1 cut(s) 1009
BseBI CCWGG 3 cut(s) 307, 726, 753
BseCI ATCGAT 1 cut(s) 968
BseDI CCNNGG 2 cut(s) 306, 416
BseGI GGATG 4 cut(s) 155, 302, 781, 823
BseJI GATNNNNATC 1 cut(s) 1021
BseLI CCNNNNNNNGG 3 cut(s) 344, 637, 713
BseMII CTCAG 1 cut(s) 983
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 732
BseSI GKGCMC 2 cut(s) 55, 216
BseXI GCAGC 4 cut(s) 485, 526, 563, 721
BsgI GTGCAG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 968
BshFI GGCC 2 cut(s) 751, 841
BshTI ACCGGT 1 cut(s) 400
BshVI ATCGAT 1 cut(s) 968
BsiEI CGRYCG 1 cut(s) 968
BsiHKAI GWGCWC 2 cut(s) 216, 657
BsiSI CCGG 2 cut(s) 401, 1010
BslFI GGGAC 3 cut(s) 48, 109, 325
BslI CCNNNNNNNGG 3 cut(s) 344, 637, 713
BsmAI GTCTC 1 cut(s) 339
BsmFI GGGAC 3 cut(s) 48, 109, 325
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 2 cut(s) 751, 841
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 3 cut(s) 55, 216, 657
Bsp13I TCCGGA 1 cut(s) 1009
Bsp143I GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 3 cut(s) 93, 588, 826
BspANI GGCC 2 cut(s) 751, 841
BspCNI CTCAG 1 cut(s) 984
BspDI ATCGAT 1 cut(s) 968
BspEI TCCGGA 1 cut(s) 1009
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 553
BspPI GGATC 3 cut(s) 181, 247, 296
BspT104I TTCGAA 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 306, 416
BssMI GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 307, 726, 753
Bst4CI ACNGT 6 cut(s) 45, 281, 355, 512, 529, 917
Bst6I CTCTTC 2 cut(s) 105, 810
BstAPI GCANNNNNTGC 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 145
BstDEI CTNAG 2 cut(s) 992, 1037
BstDSI CCRYGG 1 cut(s) 416
BstF5I GGATG 4 cut(s) 155, 302, 781, 823
BstKTI GATC 6 cut(s) 176, 255, 291, 968, 972, 1004
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
BstMCI CGRYCG 1 cut(s) 968
BstMWI GCNNNNNNNGC 6 cut(s) 186, 548, 560, 587, 671, 743
BstNI CCWGG 3 cut(s) 307, 726, 753
BstNSI RCATGY 1 cut(s) 622
BstSCI CCNGG 3 cut(s) 305, 724, 751
BstSFI CTRYAG 2 cut(s) 486, 549
BstSLI GKGCMC 2 cut(s) 55, 216
BstV1I GCAGC 4 cut(s) 485, 526, 563, 721
Bsu15I ATCGAT 1 cut(s) 968
BsuRI GGCC 2 cut(s) 751, 841
BsuTUI ATCGAT 1 cut(s) 968
BtgI CCRYGG 1 cut(s) 416
BtsCI GGATG 4 cut(s) 155, 302, 781, 823
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 2 cut(s) 525, 642
CciI TCATGA 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 386, 749
ClaI ATCGAT 1 cut(s) 968
Csp6I GTAC 2 cut(s) 608, 764
CspAI ACCGGT 1 cut(s) 400
CviAII CATG 9 cut(s) 122, 209, 377, 417, 614, 619, 632, 683, 973
CviQI GTAC 2 cut(s) 608, 764
DdeI CTNAG 2 cut(s) 992, 1037
DpnI GATC 6 cut(s) 175, 254, 290, 967, 971, 1003
DpnII GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
Eam1104I CTCTTC 2 cut(s) 105, 810
EarI CTCTTC 2 cut(s) 105, 810
Eco130I CCWWGG 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 744
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 305, 724, 751
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 9 cut(s) 125, 212, 380, 420, 617, 622, 635, 686, 976
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 3 cut(s) 48, 109, 325
FatI CATG 9 cut(s) 121, 208, 376, 416, 613, 618, 631, 682, 972
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 6 cut(s) 474, 540, 552, 588, 735, 826
FokI GGATG 4 cut(s) 142, 289, 788, 830
Fsp4HI GCNGC 6 cut(s) 474, 540, 552, 588, 735, 826
FspBI CTAG 3 cut(s) 60, 104, 315
GluI GCNGC 6 cut(s) 474, 540, 552, 588, 735, 826
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 2 cut(s) 751, 841
HapII CCGG 2 cut(s) 401, 1010
Hin1II CATG 9 cut(s) 125, 212, 380, 420, 617, 622, 635, 686, 976
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 3 cut(s) 97, 800, 1017
HpaII CCGG 2 cut(s) 401, 1010
HphI GGTGA 2 cut(s) 359, 390
Hpy166II GTNNAC 3 cut(s) 214, 382, 426
Hpy188I TCNGA 2 cut(s) 913, 1001
Hpy188III TCNNGA 4 cut(s) 27, 614, 741, 1010
Hpy8I GTNNAC 3 cut(s) 214, 382, 426
HpyAV CCTTC 3 cut(s) 631, 717, 1007
HpyCH4III ACNGT 6 cut(s) 45, 281, 355, 512, 529, 917
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 6 cut(s) 214, 413, 542, 551, 622, 858
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 548, 560, 587, 671, 743
HpyF3I CTNAG 2 cut(s) 992, 1037
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 9 cut(s) 125, 212, 380, 420, 617, 622, 635, 686, 976
Kpn2I TCCGGA 1 cut(s) 1009
Kzo9I GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
LmnI GCTCC 1 cut(s) 652
Lsp1109I GCAGC 4 cut(s) 485, 526, 563, 721
LweI GCATC 1 cut(s) 551
MaeI CTAG 3 cut(s) 60, 104, 315
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 2 cut(s) 106, 640
MalI GATC 6 cut(s) 175, 254, 290, 967, 971, 1003
MboI GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
MboII GAAGA 9 cut(s) 92, 195, 350, 537, 618, 750, 791, 797, 899
MfeI CAATTG 2 cut(s) 294, 900
MhlI GDGCHC 3 cut(s) 55, 216, 657
MluCI AATT 6 cut(s) 294, 370, 687, 782, 900, 1005
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 5 cut(s) 477, 621, 710, 844, 987
Mph1103I ATGCAT 1 cut(s) 860
MroI TCCGGA 1 cut(s) 1009
MroXI GAANNNNTTC 3 cut(s) 354, 562, 804
MslI CAYNNNNRTG 1 cut(s) 418
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 2 cut(s) 401, 1010
MspR9I CCNGG 3 cut(s) 307, 726, 753
MunI CAATTG 2 cut(s) 294, 900
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 3 cut(s) 307, 726, 753
MwoI GCNNNNNNNGC 6 cut(s) 186, 548, 560, 587, 671, 743
NcoI CCATGG 1 cut(s) 416
NdeII GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
NlaIII CATG 9 cut(s) 125, 212, 380, 420, 617, 622, 635, 686, 976
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 860
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 418
PagI TCATGA 1 cut(s) 613
PctI GAATGC 1 cut(s) 860
PdmI GAANNNNTTC 3 cut(s) 354, 562, 804
PfeI GAWTC 2 cut(s) 800, 1017
PflMI CCANNNNNTGG 1 cut(s) 344
PinAI ACCGGT 1 cut(s) 400
PkrI GCNGC 6 cut(s) 475, 541, 553, 589, 736, 827
Ple19I CGATCG 1 cut(s) 968
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 167
PsiI TTATAA 1 cut(s) 11
Psp6I CCWGG 3 cut(s) 305, 724, 751
PspGI CCWGG 3 cut(s) 305, 724, 751
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 2 cut(s) 386, 749
PstI CTGCAG 1 cut(s) 553
PvuI CGATCG 1 cut(s) 968
RsaI GTAC 2 cut(s) 609, 765
RsaNI GTAC 2 cut(s) 608, 764
RseI CAYNNNNRTG 1 cut(s) 418
SatI GCNGC 6 cut(s) 474, 540, 552, 588, 735, 826
Sau3AI GATC 6 cut(s) 173, 252, 288, 965, 969, 1001
Sau96I GGNCC 2 cut(s) 386, 749
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 3 cut(s) 307, 726, 753
SduI GDGCHC 3 cut(s) 55, 216, 657
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 2 cut(s) 486, 549
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 6 cut(s) 294, 370, 687, 782, 900, 1005
SsiI CCGC 3 cut(s) 93, 588, 826
SspMI CTAG 3 cut(s) 60, 104, 315
StyD4I CCNGG 3 cut(s) 305, 724, 751
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 6 cut(s) 45, 281, 355, 512, 529, 917
TaiI ACGT 1 cut(s) 169
TaqI TCGA 3 cut(s) 145, 740, 968
TasI AATT 6 cut(s) 294, 370, 687, 782, 900, 1005
TatI WGTACW 1 cut(s) 763
TauI GCSGC 2 cut(s) 590, 828
TfiI GAWTC 2 cut(s) 800, 1017
TscAI CASTG 2 cut(s) 532, 649
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 4 cut(s) 473, 539, 551, 734
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 3 cut(s) 291, 792, 797
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 2 cut(s) 532, 649
Van91I CCANNNNNTGG 1 cut(s) 344
VneI GTGCAC 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 386
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
XmnI GAANNNNTTC 3 cut(s) 354, 562, 804
XspI CTAG 3 cut(s) 60, 104, 315
Zsp2I ATGCAT 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.