Rh3DG054000

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007241
Physical Location & Seq
Reverse (-)
3672405 .. 3679549
7145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG054000.1

Sequence Viewer

Length: 2124 bp
ATGGTTATTTTTTCTGGAAGACAGGTGTTGTGGGCATGCTTTGACAGACTAGATCTTGGTCCATCTTCCTTCAAACATGTTCTGCTACTCGGTTATGTCAATGGCTTTCAAGTCCTTGATGTTGAAGATGCCTCTGATGTCAGTGAGCTTGTATCAAAACGTGATGATCCAGTCACATTTTTACAGATCCAGCCCCTGCCTAGAATCTCTCAAGGTCCTGAAGGATTCAGATCTTCGCATCCATTACTCATGGTTGTTGCATGTGAAGAATCAAAGAACTCTGGTATGACACAAAATGGAAGAGATGGTTTGGTAAATGGTTATACTGAGCCTCAAACAAGCAACTCTGCTATGTCTCCTAGAGTTGTTCGGTTTTATTCACTAAGGTCTCACAGTTATGTTCATGTTCTGAGATTCCGCTCGACTGTGTATATGGTTAGATGCAGTCCTCAGATAGTGGCTGTTGGTCTTGCCTCTCAAATATACTGCTTTGATGCTGTCACTCTAGAGAACAAGTTCAGTGTTCTTACTTATCCTGTCCCCCAGTTGGGAGTCCAAGGACTGGTTGGGGTTAATATTGGATATGGTCCCATGGCTGTTGGTCCCAGGTGGTTGGCTTATGCCTCCAACAACCCTTTACTGTCAAACACTTGCCGACTAAGCCCGCAAAGTCTTACACCTCCAGGTGTCAGTCCATCAACTTCACCAAGTAATGGAAATCCGGTGGCTCGGTATGCCATGGAATCTAGTAAGCAGTTAGCTGCTGGGTTGCTAAATCTGGGAGACATGGGCTACAAAACTTTGTCTAAGTACTATCAACCAGATGGTTCCAGCTCTCCAGTGTCATCAAATTCTATCTGGAAAGTTGGGAAGGTTGGATCACATTCAACAGAAACAGATATTGCTGGAATGGTTGTTGTAAAAGACATTGTTTCCAGGGCGGTCGTTTCACAATTTAGGGCACATACTAGTCCCATTTCTGCTCTATGTTTTGACCCAAGTGGGACTCTCCTTGTTACTGCCTCGATACATGGAAACAATATTAACATTTTTCGGATAATGCCATCACGTACTGGATCTGGCACCCAGAACTATGACTTGAATTCCTCTCATGTGCACCTTTACAAGCTCCATCGTGGCATGACATCAGCTGTAATCCAAGACATATGTTTTAGTCAATACAGTCAATGGATTACAATTGTTTCATCGAGGGGAACTTGCCATATTTTTACTTTTTCCCCCTTTGGTGGTGATACTACTCACAAGCAGAGTTCTCATGTTGATGGGCCTAGTCATTTGCCAGTTCCGTCTGTACCGTGGTGGTTCACTCCTTATTTCCTGACAAACCAGCAACTTTTTTCTCCACCACCACCCCCTGTTACCCTTTCTGTGGTTAGCAGAATAAAAGATAACTCCGGGTGGATCAATACAGTTAGTCATGCTGCTTCTTCTGCAGCCGGAAAAGCTTCCATTCCATCCGGCGCTGTTACTGCTGTATTTCATAATTCTGTTGCTCATGATCTGCAAACTTCTCATTTAAAGGTAACAGCTCTGGAGCACCTATTGGTTTACACTCCTTCTGGTCATGCAGTACAGTTTAAACTACTTCCAAGAGTTGGGATAGAGCCTGGTGAAGCTACATCAAGAACAGTGCCAGGCTCTTCAGTACAAATACAGGATGAGGAGTTGCGAGTGAAAGTTGAACCTCTGCAGTGGTGGGATGTTTGCCGAAGAACTGATTGGCCTGAAAGAGAGGAATGCATTTCTGGGATTAAACTTGGGAGACAAGAAGATGAGGAGACAGTCATGGATACTTTTGATTGTGATGTTAATGATATTGGGGATAAGGAGTCGGTGAAACCGCTTGAGCGATCTCACTTGTATCTCGCCAACGCTGAGGTTCAGATTAACTGTGGAAGGATTCCAATCTGGCAAAAGTCTAAGATATATTTCTATACAATGAGTACCCTGGGAGCCATTGAACAAAATTTTACTAAAGATCTTACTGGAGGAGAGATTGAAATAGAAAAGGTTCCTGTTCACGAGGTCGAAATTAGGCGGAAGGATTTGCTACCTGTTTCTGATCAATTCCAAAGAGTGCAGTCTAGTTGGAGTGACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

707

Amino Acids

77.83

Weight (kDa)

6.74

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BCAS3_WD40 PF21034 27 - 420 1e-35 BCAS3, WD40 repeat
BCAS3 PF12490 556 - 693 1.9e-47 BCAS3 microtubule associated cell migration factor, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1082
AccB7I CCANNNNNTGG 3 cut(s) 562, 713, 1616
AccBSI CCGCTC 1 cut(s) 420
AciI CCGC 5 cut(s) 418, 665, 941, 1862, 2059
AclWI GGATC 5 cut(s) 161, 181, 886, 1084, 1430
AcsI RAATTY 3 cut(s) 850, 1102, 1987
AcuI CTGAAG 2 cut(s) 240, 1647
AfaI GTAC 6 cut(s) 812, 1072, 1314, 1593, 1668, 1966
AfiI CCNNNNNNNGG 7 cut(s) 547, 548, 562, 713, 1247, 1390, 1616
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 8 cut(s) 73, 110, 125, 888, 1102, 1703, 1982, 2021
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 6 cut(s) 605, 682, 935, 1627, 1654, 1968
AjuI GAANNNNNNNTTGG 2 cut(s) 1723, 1755
AluBI AGCT 8 cut(s) 148, 761, 834, 1129, 1151, 1466, 1550, 1637
AluI AGCT 8 cut(s) 148, 761, 834, 1129, 1151, 1466, 1550, 1637
Alw21I GWGCWC 2 cut(s) 1119, 1560
Alw26I GTCTC 5 cut(s) 360, 393, 777, 1777, 1793
Alw44I GTGCAC 1 cut(s) 1115
AlwI GGATC 5 cut(s) 161, 181, 886, 1084, 1430
AlwNI CAGNNNCTG 1 cut(s) 196
AoxI GGCC 2 cut(s) 1286, 1742
ApaLI GTGCAC 1 cut(s) 1115
ApeKI GCWGC 3 cut(s) 761, 1442, 1454
ApoI RAATTY 3 cut(s) 850, 1102, 1987
Asp700I GAANNNNTTC 3 cut(s) 515, 1465, 2031
AspLEI GCGC 1 cut(s) 1484
AspS9I GGNCC 5 cut(s) 59, 215, 587, 602, 1286
AsuC2I CCSGG 1 cut(s) 1417
AsuHPI GGTGA 4 cut(s) 696, 1262, 1643, 1867
AvaII GGWCC 4 cut(s) 59, 215, 587, 602
BaeGI GKGCMC 2 cut(s) 964, 1119
BanI GGYRCC 1 cut(s) 1082
BauI CACGAG 1 cut(s) 2042
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 2 cut(s) 1119, 1560
BbvCI CCTCAGC 1 cut(s) 1896
BbvI GCAGC 3 cut(s) 748, 1429, 1466
BccI CCATC 8 cut(s) 70, 299, 703, 818, 1072, 1140, 1277, 1483
BciT130I CCWGG 6 cut(s) 607, 684, 937, 1629, 1656, 1970
BciVI GTATCC 1 cut(s) 1804
BclI TGATCA 1 cut(s) 2083
BcnI CCSGG 1 cut(s) 1417
BcoDI GTCTC 5 cut(s) 360, 393, 777, 1777, 1793
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 8 cut(s) 50, 201, 360, 506, 747, 969, 1290, 2106
BfmI CTRYAG 2 cut(s) 1452, 1709
BfoI RGCGCY 1 cut(s) 1485
BfuI GTATCC 1 cut(s) 1804
BglII AGATCT 3 cut(s) 52, 230, 1999
BisI GCNGC 3 cut(s) 762, 1443, 1455
BlsI GCNGC 3 cut(s) 763, 1444, 1456
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 7 cut(s) 607, 684, 937, 1417, 1629, 1656, 1970
Bme18I GGWCC 4 cut(s) 59, 215, 587, 602
BmgT120I GGNCC 5 cut(s) 59, 215, 587, 602, 1286
BmiI GGNNCC 6 cut(s) 589, 604, 829, 1084, 1975, 2034
BmrFI CCNGG 7 cut(s) 607, 684, 937, 1417, 1629, 1656, 1970
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 4 cut(s) 118, 247, 431, 484
BmuI ACTGGG 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 4 cut(s) 666, 822, 1574, 2028
Bpu10I CCTNAGC 1 cut(s) 1896
BpuEI CTTGAG 2 cut(s) 195, 1886
BpuMI CCSGG 1 cut(s) 1417
BsaAI YACGTR 1 cut(s) 1070
BsaBI GATNNNNATC 2 cut(s) 229, 1925
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 8 cut(s) 556, 591, 605, 738, 936, 1316, 1968, 1969
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 7 cut(s) 547, 548, 562, 713, 1247, 1390, 1616
Bse1I ACTGG 7 cut(s) 170, 544, 567, 839, 1078, 1301, 2011
Bse8I GATNNNNATC 2 cut(s) 229, 1925
BseBI CCWGG 6 cut(s) 607, 684, 937, 1629, 1656, 1970
BseDI CCNNGG 8 cut(s) 556, 591, 605, 738, 936, 1316, 1968, 1969
BseGI GGATG 4 cut(s) 238, 1475, 1684, 1726
BseJI GATNNNNATC 2 cut(s) 229, 1925
BseLI CCNNNNNNNGG 7 cut(s) 547, 548, 562, 713, 1247, 1390, 1616
BseMII CTCAG 4 cut(s) 318, 401, 464, 1887
BseNI ACTGG 7 cut(s) 170, 544, 567, 839, 1078, 1301, 2011
BseRI GAGGAG 3 cut(s) 1697, 1811, 2025
BseSI GKGCMC 2 cut(s) 964, 1119
BseXI GCAGC 3 cut(s) 748, 1429, 1466
BseYI CCCAGC 1 cut(s) 764
BsgI GTGCAG 1 cut(s) 2120
Bsh1285I CGRYCG 1 cut(s) 945
BshFI GGCC 2 cut(s) 1288, 1744
BshNI GGYRCC 1 cut(s) 1082
BsiEI CGRYCG 1 cut(s) 945
BsiHKAI GWGCWC 2 cut(s) 1119, 1560
BsiSI CCGG 4 cut(s) 722, 1416, 1458, 1479
BslFI GGGAC 5 cut(s) 524, 573, 588, 957, 1018
BslI CCNNNNNNNGG 7 cut(s) 547, 548, 562, 713, 1247, 1390, 1616
BsmAI GTCTC 5 cut(s) 360, 393, 777, 1777, 1793
BsmFI GGGAC 5 cut(s) 524, 573, 588, 957, 1018
BsmI GAATGC 1 cut(s) 1763
BsnI GGCC 2 cut(s) 1288, 1744
Bso31I GGTCTC 1 cut(s) 393
Bsp1286I GDGCHC 3 cut(s) 964, 1119, 1560
Bsp19I CCATGG 2 cut(s) 591, 738
BspACI CCGC 5 cut(s) 418, 665, 941, 1862, 2059
BspANI GGCC 2 cut(s) 1288, 1744
BspCNI CTCAG 4 cut(s) 319, 402, 463, 1888
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 6 cut(s) 589, 604, 829, 1084, 1975, 2034
BspMAI CTGCAG 2 cut(s) 1456, 1713
BspPI GGATC 5 cut(s) 161, 181, 886, 1084, 1430
BspQI GCTCTTC 1 cut(s) 1666
BspT107I GGYRCC 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 420
BsrI ACTGG 7 cut(s) 170, 544, 567, 839, 1078, 1301, 2011
BssECI CCNNGG 8 cut(s) 556, 591, 605, 738, 936, 1316, 1968, 1969
BssSI CACGAG 1 cut(s) 2042
BssT1I CCWWGG 3 cut(s) 556, 591, 738
Bst2BI CACGAG 1 cut(s) 2042
Bst2UI CCWGG 6 cut(s) 607, 684, 937, 1629, 1656, 1970
Bst6I CTCTTC 2 cut(s) 295, 1666
BstBAI YACGTR 1 cut(s) 1070
BstC8I GCNNGC 2 cut(s) 37, 665
BstDEI CTNAG 8 cut(s) 327, 383, 410, 450, 659, 807, 1896, 1941
BstDSI CCRYGG 3 cut(s) 591, 738, 1316
BstF5I GGATG 4 cut(s) 238, 1475, 1684, 1726
BstH2I RGCGCY 1 cut(s) 1485
BstHHI GCGC 1 cut(s) 1484
BstMAI GTCTC 5 cut(s) 360, 393, 777, 1777, 1793
BstMCI CGRYCG 1 cut(s) 945
BstMWI GCNNNNNNNGC 5 cut(s) 660, 734, 1451, 1463, 1490
BstNI CCWGG 6 cut(s) 607, 684, 937, 1629, 1656, 1970
BstNSI RCATGY 3 cut(s) 39, 80, 264
BstSCI CCNGG 7 cut(s) 605, 682, 935, 1415, 1627, 1654, 1968
BstSFI CTRYAG 2 cut(s) 1452, 1709
BstSLI GKGCMC 2 cut(s) 964, 1119
BstV1I GCAGC 3 cut(s) 748, 1429, 1466
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 5 cut(s) 52, 186, 230, 1076, 1999
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 5 cut(s) 52, 186, 230, 1076, 1999
BsuI GTATCC 1 cut(s) 1804
BsuRI GGCC 2 cut(s) 1288, 1744
BtgI CCRYGG 3 cut(s) 591, 738, 1316
BtsCI GGATG 4 cut(s) 238, 1475, 1684, 1726
BtsI GCAGTG 1 cut(s) 1718
BtsIMutI CAGTG 5 cut(s) 148, 526, 846, 1656, 1718
Cac8I GCNNGC 2 cut(s) 37, 665
CaiI CAGNNNCTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 1516
CfoI GCGC 1 cut(s) 1484
Cfr13I GGNCC 5 cut(s) 59, 215, 587, 602, 1286
Csp6I GTAC 6 cut(s) 811, 1071, 1313, 1592, 1667, 1965
CviQI GTAC 6 cut(s) 811, 1071, 1313, 1592, 1667, 1965
DdeI CTNAG 8 cut(s) 327, 383, 410, 450, 659, 807, 1896, 1941
DraI TTTAAA 2 cut(s) 1539, 1600
Eam1104I CTCTTC 2 cut(s) 295, 1666
EarI CTCTTC 2 cut(s) 295, 1666
EciI GGCGGA 1 cut(s) 2074
Eco130I CCWWGG 3 cut(s) 556, 591, 738
Eco31I GGTCTC 1 cut(s) 393
Eco47I GGWCC 4 cut(s) 59, 215, 587, 602
Eco57I CTGAAG 2 cut(s) 240, 1647
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 1102
EcoRII CCWGG 6 cut(s) 605, 682, 935, 1627, 1654, 1968
EcoT14I CCWWGG 3 cut(s) 556, 591, 738
EcoT22I ATGCAT 1 cut(s) 1763
ErhI CCWWGG 3 cut(s) 556, 591, 738
FaqI GGGAC 5 cut(s) 524, 573, 588, 957, 1018
FauI CCCGC 1 cut(s) 672
FauNDI CATATG 1 cut(s) 1166
FbaI TGATCA 1 cut(s) 2083
Fnu4HI GCNGC 3 cut(s) 762, 1443, 1455
FokI GGATG 4 cut(s) 225, 1462, 1691, 1733
Fsp4HI GCNGC 3 cut(s) 762, 1443, 1455
FspBI CTAG 8 cut(s) 50, 201, 360, 506, 747, 969, 1290, 2106
GlaI GCGC 1 cut(s) 1483
GluI GCNGC 3 cut(s) 762, 1443, 1455
GsaI CCCAGC 1 cut(s) 768
GsuI CTGGAG 4 cut(s) 666, 822, 1574, 2028
HaeII RGCGCY 1 cut(s) 1485
HaeIII GGCC 2 cut(s) 1288, 1744
HapII CCGG 4 cut(s) 722, 1416, 1458, 1479
HhaI GCGC 1 cut(s) 1484
Hin6I GCGC 1 cut(s) 1482
HinP1I GCGC 1 cut(s) 1482
HindIII AAGCTT 1 cut(s) 1464
HinfI GANTC 9 cut(s) 204, 225, 269, 414, 552, 743, 1006, 1850, 1921
HpaII CCGG 4 cut(s) 722, 1416, 1458, 1479
HphI GGTGA 4 cut(s) 696, 1262, 1643, 1867
Hpy166II GTNNAC 4 cut(s) 1117, 1326, 1570, 2041
Hpy188I TCNGA 7 cut(s) 136, 230, 411, 453, 1056, 1905, 2083
Hpy188III TCNNGA 9 cut(s) 15, 218, 506, 859, 1339, 1517, 1553, 1644, 2042
Hpy8I GTNNAC 4 cut(s) 1117, 1326, 1570, 2041
HpyAV CCTTC 6 cut(s) 79, 215, 865, 1587, 1911, 2056
HpyCH4IV ACGT 2 cut(s) 160, 1069
HpyCH4V TGCA 9 cut(s) 260, 444, 1117, 1454, 1525, 1589, 1711, 1761, 2101
HpyF10VI GCNNNNNNNGC 5 cut(s) 660, 734, 1451, 1463, 1490
HpyF3I CTNAG 8 cut(s) 327, 383, 410, 450, 659, 807, 1896, 1941
HpySE526I ACGT 2 cut(s) 160, 1069
HspAI GCGC 1 cut(s) 1482
Ksp22I TGATCA 1 cut(s) 2083
LguI GCTCTTC 1 cut(s) 1666
LmnI GCTCC 3 cut(s) 1134, 1555, 1973
Lsp1109I GCAGC 3 cut(s) 748, 1429, 1466
LweI GCATC 4 cut(s) 118, 247, 431, 484
MaeI CTAG 8 cut(s) 50, 201, 360, 506, 747, 969, 1290, 2106
MaeII ACGT 2 cut(s) 160, 1069
MaeIII GTNAC 7 cut(s) 172, 499, 1015, 1378, 1486, 1543, 2114
MbiI CCGCTC 1 cut(s) 420
MfeI CAATTG 1 cut(s) 1197
MflI RGATCY 5 cut(s) 52, 186, 230, 1076, 1999
MhlI GDGCHC 3 cut(s) 964, 1119, 1560
MluCI AATT 8 cut(s) 850, 953, 1102, 1197, 1504, 1987, 2052, 2087
MlyI GAGTC 3 cut(s) 561, 1000, 1859
MmeI TCCRAC 3 cut(s) 651, 856, 2090
Mph1103I ATGCAT 1 cut(s) 1763
MroXI GAANNNNTTC 3 cut(s) 515, 1465, 2031
MseI TTAA 7 cut(s) 573, 1044, 1538, 1599, 1773, 1830, 1908
MslI CAYNNNNRTG 2 cut(s) 396, 1281
MspA1I CMGCKG 1 cut(s) 1151
MspI CCGG 4 cut(s) 722, 1416, 1458, 1479
MspR9I CCNGG 7 cut(s) 607, 684, 937, 1417, 1629, 1656, 1970
MssI GTTTAAAC 1 cut(s) 1600
MunI CAATTG 1 cut(s) 1197
Mva1269I GAATGC 1 cut(s) 1763
MvaI CCWGG 6 cut(s) 607, 684, 937, 1629, 1656, 1970
MwoI GCNNNNNNNGC 5 cut(s) 660, 734, 1451, 1463, 1490
NciI CCSGG 1 cut(s) 1417
NcoI CCATGG 2 cut(s) 591, 738
NdeI CATATG 1 cut(s) 1166
NlaIV GGNNCC 6 cut(s) 589, 604, 829, 1084, 1975, 2034
NmuCI GTSAC 3 cut(s) 172, 499, 2114
NsiI ATGCAT 1 cut(s) 1763
NspI RCATGY 3 cut(s) 39, 80, 264
PaeI GCATGC 1 cut(s) 39
PagI TCATGA 1 cut(s) 1516
PasI CCCWGGG 1 cut(s) 1969
PciI ACATGT 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 1666
PctI GAATGC 1 cut(s) 1763
PdmI GAANNNNTTC 3 cut(s) 515, 1465, 2031
PfeI GAWTC 6 cut(s) 204, 225, 269, 414, 743, 1921
PflMI CCANNNNNTGG 3 cut(s) 562, 713, 1616
PkrI GCNGC 3 cut(s) 763, 1444, 1456
PleI GAGTC 3 cut(s) 560, 1000, 1858
PmeI GTTTAAAC 1 cut(s) 1600
PpsI GAGTC 3 cut(s) 560, 1000, 1858
Ppu21I YACGTR 1 cut(s) 1070
PpuMI RGGWCCY 1 cut(s) 215
PscI ACATGT 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 6 cut(s) 605, 682, 935, 1627, 1654, 1968
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 6 cut(s) 605, 682, 935, 1627, 1654, 1968
PspN4I GGNNCC 6 cut(s) 589, 604, 829, 1084, 1975, 2034
PspPI GGNCC 5 cut(s) 59, 215, 587, 602, 1286
PspPPI RGGWCCY 1 cut(s) 215
PstI CTGCAG 2 cut(s) 1456, 1713
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 5 cut(s) 52, 186, 230, 1076, 1999
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 6 cut(s) 812, 1072, 1314, 1593, 1668, 1966
RsaNI GTAC 6 cut(s) 811, 1071, 1313, 1592, 1667, 1965
RseI CAYNNNNRTG 2 cut(s) 396, 1281
SapI GCTCTTC 1 cut(s) 1666
SaqAI TTAA 7 cut(s) 573, 1044, 1538, 1599, 1773, 1830, 1908
SatI GCNGC 3 cut(s) 762, 1443, 1455
Sau96I GGNCC 5 cut(s) 59, 215, 587, 602, 1286
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 3 cut(s) 561, 1000, 1859
ScrFI CCNGG 7 cut(s) 607, 684, 937, 1417, 1629, 1656, 1970
SduI GDGCHC 3 cut(s) 964, 1119, 1560
SfaNI GCATC 4 cut(s) 118, 247, 431, 484
SfcI CTRYAG 2 cut(s) 1452, 1709
SinI GGWCC 4 cut(s) 59, 215, 587, 602
SmiMI CAYNNNNRTG 2 cut(s) 396, 1281
SmlI CTYRAG 2 cut(s) 210, 1865
SmoI CTYRAG 2 cut(s) 210, 1865
SpeI ACTAGT 1 cut(s) 968
SphI GCATGC 1 cut(s) 39
Sse9I AATT 8 cut(s) 850, 953, 1102, 1197, 1504, 1987, 2052, 2087
SsiI CCGC 5 cut(s) 418, 665, 941, 1862, 2059
SspI AATATT 2 cut(s) 577, 1042
SspMI CTAG 8 cut(s) 50, 201, 360, 506, 747, 969, 1290, 2106
StyD4I CCNGG 7 cut(s) 605, 682, 935, 1415, 1627, 1654, 1968
StyI CCWWGG 3 cut(s) 556, 591, 738
TaiI ACGT 2 cut(s) 163, 1072
TaqI TCGA 4 cut(s) 422, 1025, 1208, 2049
TasI AATT 8 cut(s) 850, 953, 1102, 1197, 1504, 1987, 2052, 2087
TatI WGTACW 3 cut(s) 810, 1591, 1666
TfiI GAWTC 6 cut(s) 204, 225, 269, 414, 743, 1921
Tru1I TTAA 7 cut(s) 573, 1044, 1538, 1599, 1773, 1830, 1908
Tru9I TTAA 7 cut(s) 573, 1044, 1538, 1599, 1773, 1830, 1908
TscAI CASTG 5 cut(s) 148, 526, 846, 1656, 1718
TseFI GTSAC 3 cut(s) 172, 499, 2114
TseI GCWGC 3 cut(s) 761, 1442, 1454
Tsp45I GTSAC 3 cut(s) 172, 499, 2114
TspDTI ATGAA 3 cut(s) 392, 1194, 1490
TspGWI ACGGA 1 cut(s) 1296
TspRI CASTG 5 cut(s) 148, 526, 846, 1656, 1718
Van91I CCANNNNNTGG 3 cut(s) 562, 713, 1616
VneI GTGCAC 1 cut(s) 1115
VpaK11BI GGWCC 4 cut(s) 59, 215, 587, 602
XapI RAATTY 3 cut(s) 850, 1102, 1987
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 3 cut(s) 39, 80, 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmnI GAANNNNTTC 3 cut(s) 515, 1465, 2031
XspI CTAG 8 cut(s) 50, 201, 360, 506, 747, 969, 1290, 2106
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.