pycom12g21120

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007241
Physical Location & Seq
Forward (+)
22371193 .. 22377142
5950 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21120.8

Sequence Viewer

Length: 2955 bp
ATGAAGAAGAACCGAAACGGCGGCACTAAGGTTGTTCAGAGTAATCAACATCAACACGCCAATAACAATGGCTTCCTTCCGAATTCTTTCAAATTCATTTCTTCTTGCATTAAAACTGCATCTTCCGGCGTAAGGTCGGGCGCCGCCTCCGTCGCCGCCTCCATCTCCGTCGATTCTCACGATTGCCGAGACCAGGTGTTGTGGGCTTGCTTCGACAAAGTAGAGCTTGGTCCATCTTCCTTCAAACATGTTCTGCTACTTGGTTATTCCAATGGATTTCAAGTCCTTGAAGTTGAAGATGCCTCTAATTTCAGTGAACTTGTCTCAAGGCGTGATGATCCAGCTCCATTTTTACAGATGCAGCCCCTGCCTGCAAAGTGTGAGGGTCATGAAGGATTCAGATCCTCACATCCTATACTCATGGTTGTTGCATGTGATGAAAAGCGCTCAGGTACGATGCAAACAGGGAGAGATGGGTTGGCCAATGGTCATTCTGAGCCTCAAACAGGAAACTCTGCTTTGTCTCCTACAGCTGTTCGGTTTTATTCGCTAAGGTCGTGCAGTTATGTTCATGTTCTAAGATTCCGTTCAACTGTGTATATGGTTAGATGCAGTCCTCAAGTAGTAGCTGTGGGTCTTGCATCCCAAATATACTGCTTCGATGCTGTCACCCTTGAGAACAAATTCAGTGTCCTCACTTATCCTGTCCCCCAGTTGGGAGTTCAAGGACTGGTTGGGGTCAATATCGGGTATGGTCCTATGGCTGTTGGTCCCAGGTGGTTGGCTTATGCCTCCAACAACCCTCTGATGTCAAACACAGGCCGCCTCAGTCCTCAAAGTCTTACTCCACCTGGTGTAAGTCCATCAACTTCGCCAAGTAGTGGAAGTCTGATGGCTCGATATGCAATGGAATCTAGTAAACAGTTAGCTTCTGGGTTACTTAATCTGGGAGACATGGGTTACAAAACTTTGTCCAAGTACTATCAAGAGATCGATCTAACACCAGATGGTTCTAGCTCTCCTGTGTCATCAAATTCTAGCTGGAAAGTTGGGAGGGTTGCATCACATTCAACCGAGACAGATATTGCTGGAATGGTTGTTATAAAAGACTTTGTTTCCAGAGCAGTTGTGTCACAATTTAGGGCACATACCAGTCCCATTTCTGCCCTGTGTTTTGACCCCAGTGGGACTCTTCTAGTTACTGCCTCAATACATGGAAACAATATAAACATTTTTCGAATAATGCCATCCTGCTCGCGTAATGGATCATGCACTCAAAGCTTTGACTGGACTTCTTCTCATGTGCACCTTTACAAACTACATCGTGGCATAACGTCTGCTGTGATCCAAGACATTTGTTTTAGTCAATACAGTCAATGGATTACAATTGTTACATCCAGGGGAACTAGCCATATTTTTGCTTTATCTCCCTTCGGTGGTGACACTGTTTTGCAAATACATTCCCATATTGACGGACCTACACTTTCAATGGTTCCATCTGCACCATGGTGGTTTACTCCATATTTCACAAGAAGTCAGCAAACCTTTTCTCCACCTCCAGCAGCTACCCTTTCTGTGGTTAGCAGAATAAAAAATAACTCCGGGTGGCTCAACACTGTTAGTAATGCAGCATCTTCTGCAGCAGGAAAGGGTTCCTTTCGATCTGGGGCTGTAGCCGCTGTATTCCATAGTTCCGTACCTGATGGTCTGCAATCTTTCCATGCAAAGGTCACTGCATTGGAGCACTTATTGGCTTATACTGCTTCTGGTCATGTAATTCAGTATAAACTACTGCCTTCATTGGGAGGAGAGGCAGGTGACACTGTTTCAGGAGCTGGGCCAGGTTCTTCAGTTCAAATACAGGATGAAGATTTGCGAGTGAAAGTTGAATCTCTTCATTTCTTGGATGTTTGCCGAAGAAATGATTGGCCTGAAAGAGAGGAAGGCATTTCTGGATCGATCATACTTGAGAAACAAGACGATATTGACAGAATTGATAGTTCTGACTGTGATGACAATGATGTTGGGGATAAGGAGTTGGCAAAACCCTTTGAACGATCTCACGTTTACCTCTCTAATGCTGAGGTTCAGATCAATTCTGGAAGGATTCCAATCTGGCAAAAATCTAAGATATATTTCTATACAATGAGTCCGTCTGCTGCTGGTGAGCTAAATTTTGGTAAAGATCTTACCGGCGGAGAGATGGAAATAGAGAAGTTTCCTGTTCACGAGGTTGAAATGAGGCAGAAAGATTTGCTACCTGTTGTTCATCCTTTTCATAGGTTTCAGTCTGACTGGAATGGCAGGCATCTAATTGGAGGAGGATGCTCAAGTTCATCTTCTGATTCTCATGAAGATAAAGAAAAGTTTCAGGAAAATTCTGTTAGTTCTGGTCAGAATTTGACACCTATAGGCTCAGCTGAAAACCCAAATGCTGGTGATTATCCATTTATTCTCCAACCTGGAAATGGTCAGAATGGCGAAAAAAGAGGGAGATCATTTTCGGTATCACCTCACATGAACTATACCTCCACAAAGAAAAATATTACTTCAGTATCCCTGAAACAACCGACCTCAGGTGTTTCTCTTGGTGACGATTGTAATTTTTCAAATAGTCTATCCACTTTAACAAGCGGCTCCCTTTCAGCCGATAGAACTATTGCAACAAAGGTTCAGTCGGTGAATAGTGGTGGGGCCAGTGAAGGTTACAATGTAGGTTCTAACCGTTCTGATCTGAGTATGAACATGCTCGATGAAGGACCAGTACACGAATCACCGGATTTTGAGCCATTTTTCCAAGAGGGGTATTGTAAAGAATCACCTCTGAGCAACTTTCGTGAGTCGACTGAAGTTGGAACTGATGTTGACAGCCCCTGTGATAGGGAGAAAAGCGAGGAAGATGGTGACAATGATGACATGCTTGGTGGTGTTTTTGCCTTCTCCGAGGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000785 GO:0001952 GO:0001953 GO:0003674 GO:0003682 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006357 GO:0006810 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007030 GO:0007162 GO:0007275 GO:0008047 GO:0008092 GO:0008134 GO:0008150 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010256 GO:0010468 GO:0010556 GO:0010557 GO:0010594 GO:0010595 GO:0010604 GO:0010628 GO:0010632 GO:0010634 GO:0010638 GO:0010698 GO:0010810 GO:0010812 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0019219 GO:0019222 GO:0019899 GO:0030155 GO:0030234 GO:0030334 GO:0030335 GO:0031023 GO:0031252 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031344 GO:0031346 GO:0031667 GO:0031974 GO:0031981 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032878 GO:0032879 GO:0032880 GO:0032956 GO:0032970 GO:0033043 GO:0033157 GO:0034260 GO:0035035 GO:0035148 GO:0035239 GO:0035257 GO:0035295 GO:0035327 GO:0040012 GO:0040017 GO:0042221 GO:0042393 GO:0042594 GO:0043085 GO:0043086 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043547 GO:0043627 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045111 GO:0045893 GO:0045935 GO:0045944 GO:0048487 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051346 GO:0051427 GO:0051489 GO:0051491 GO:0051493 GO:0051495 GO:0051716 GO:0051893 GO:0051895 GO:0060255 GO:0060341 GO:0060491 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070887 GO:0071391 GO:0071840 GO:0080090 GO:0090087 GO:0090109 GO:0090316 GO:0090630 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120032 GO:0120034 GO:0120035 GO:1901888 GO:1901889 GO:1902680 GO:1903391 GO:1903392 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:2000112 GO:2000114 GO:2000145 GO:2000147 GO:2000249 GO:2000251 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BCAS3 WD40

Protein Analysis

985

Amino Acids

106.37

Weight (kDa)

5.8

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1103
AarI CACCTGC 1 cut(s) 1805
Acc36I ACCTGC 1 cut(s) 1805
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 2 cut(s) 881, 2435
AccI GTMKAC 1 cut(s) 2843
AccII CGCG 1 cut(s) 1258
AciI CCGC 7 cut(s) 21, 144, 156, 823, 1677, 2196, 2634
AclWI GGATC 5 cut(s) 332, 396, 1273, 1339, 1963
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 7 cut(s) 82, 92, 683, 1033, 2173, 2377, 2398
AcuI CTGAAG 3 cut(s) 1833, 2535, 2868
AcyI GRCGYC 1 cut(s) 141
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 4 cut(s) 454, 980, 1698, 2766
AfeI AGCGCT 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 247
AjnI CCWGG 6 cut(s) 192, 773, 850, 1397, 1840, 2461
AjuI GAANNNNNNNTTGG 2 cut(s) 1909, 1941
AleI CACNNNNGTG 1 cut(s) 1507
AloI GAACNNNNNNTCC 2 cut(s) 2513, 2545
Alw21I GWGCWC 2 cut(s) 1308, 1746
Alw26I GTCTC 5 cut(s) 183, 328, 528, 945, 1070
Alw44I GTGCAC 1 cut(s) 1304
AlwI GGATC 5 cut(s) 332, 396, 1273, 1339, 1963
AlwNI CAGNNNCTG 3 cut(s) 367, 1835, 2874
Aor51HI AGCGCT 1 cut(s) 446
AoxI GGCC 5 cut(s) 480, 820, 1838, 1928, 2694
ApaLI GTGCAC 1 cut(s) 1304
ApeKI GCWGC 5 cut(s) 361, 1562, 1628, 1640, 2159
ApoI RAATTY 7 cut(s) 82, 92, 683, 1033, 2173, 2377, 2398
ArsI GACNNNNNNTTYG 4 cut(s) 675, 707, 2660, 2692
Asp700I GAANNNNTTC 5 cut(s) 86, 683, 1651, 1893, 2367
AspLEI GCGC 2 cut(s) 143, 447
AspS9I GGNCC 7 cut(s) 230, 755, 770, 1475, 1838, 2694, 2759
AsuC2I CCSGG 1 cut(s) 1603
AsuII TTCGAA 1 cut(s) 1237
AvaII GGWCC 5 cut(s) 230, 755, 770, 1475, 2759
AxyI CCTNAGG 1 cut(s) 2575
BaeGI GKGCMC 2 cut(s) 1147, 1308
BaeI ACNNNNGTAYC 2 cut(s) 2538, 2571
BalI TGGCCA 1 cut(s) 482
BanI GGYRCC 1 cut(s) 140
BarI GAAGNNNNNNTAC 2 cut(s) 2748, 2780
BauI CACGAG 1 cut(s) 2228
Bbv12I GWGCWC 2 cut(s) 1308, 1746
BbvCI CCTCAGC 1 cut(s) 2082
BbvI GCAGC 5 cut(s) 373, 1574, 1640, 1652, 2146
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 6 cut(s) 194, 775, 852, 1399, 1842, 2463
BciVI GTATCC 1 cut(s) 2566
BcnI CCSGG 1 cut(s) 1603
BcoDI GTCTC 5 cut(s) 183, 328, 528, 945, 1070
BfaI CTAG 5 cut(s) 915, 1014, 1038, 1196, 1407
BfmI CTRYAG 4 cut(s) 528, 1638, 1671, 2409
BfoI RGCGCY 2 cut(s) 144, 448
BfuAI ACCTGC 1 cut(s) 1805
BfuI GTATCC 1 cut(s) 2566
BglII AGATCT 1 cut(s) 2185
BlpI GCTNAGC 1 cut(s) 2416
BmcAI AGTACT 1 cut(s) 980
Bme1390I CCNGG 7 cut(s) 194, 775, 852, 1399, 1603, 1842, 2463
Bme18I GGWCC 5 cut(s) 230, 755, 770, 1475, 2759
BmgT120I GGNCC 7 cut(s) 230, 755, 770, 1475, 1838, 2694, 2759
BmiI GGNNCC 6 cut(s) 142, 772, 1494, 1654, 2638, 2695
BmrFI CCNGG 7 cut(s) 194, 775, 852, 1399, 1603, 1842, 2463
BmrI ACTGGG 2 cut(s) 706, 1176
BmuI ACTGGG 2 cut(s) 706, 1176
BpmI CTGGAG 1 cut(s) 1542
Bpu10I CCTNAGC 3 cut(s) 448, 551, 2082
Bpu1102I GCTNAGC 1 cut(s) 2416
Bpu14I TTCGAA 1 cut(s) 1237
BpuEI CTTGAG 5 cut(s) 310, 603, 695, 1988, 2314
BpuMI CCSGG 1 cut(s) 1603
Bsa29I ATCGAT 2 cut(s) 993, 1958
BsaBI GATNNNNATC 2 cut(s) 400, 2111
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 773, 1398, 1505, 2943
BsaWI WCCGGW 1 cut(s) 2776
BsaXI ACNNNNNCTCC 4 cut(s) 1534, 1564, 2513, 2543
Bse118I RCCGGY 1 cut(s) 2192
Bse1I ACTGG 8 cut(s) 712, 735, 1152, 1182, 1292, 2300, 2697, 2762
Bse21I CCTNAGG 1 cut(s) 2575
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 2 cut(s) 400, 2111
BseBI CCWGG 6 cut(s) 194, 775, 852, 1399, 1842, 2463
BseCI ATCGAT 2 cut(s) 993, 1958
BseDI CCNNGG 4 cut(s) 773, 1398, 1505, 2943
BseGI GGATG 8 cut(s) 409, 641, 1247, 1394, 1870, 1912, 2269, 2330
BseJI GATNNNNATC 2 cut(s) 400, 2111
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 8 cut(s) 462, 486, 841, 2073, 2430, 2589, 2726, 2816
BseNI ACTGG 8 cut(s) 712, 735, 1152, 1182, 1292, 2300, 2697, 2762
BseRI GAGGAG 2 cut(s) 1821, 2334
BseSI GKGCMC 2 cut(s) 1147, 1308
BseXI GCAGC 5 cut(s) 373, 1574, 1640, 1652, 2146
BseYI CCCAGC 1 cut(s) 1835
BsgI GTGCAG 2 cut(s) 580, 1485
Bsh1236I CGCG 1 cut(s) 1258
BshFI GGCC 5 cut(s) 482, 822, 1840, 1930, 2696
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 2 cut(s) 993, 1958
BsiHKAI GWGCWC 2 cut(s) 1308, 1746
BsiSI CCGG 4 cut(s) 126, 1602, 2193, 2777
BslFI GGGAC 4 cut(s) 692, 756, 1140, 1201
BsmAI GTCTC 5 cut(s) 183, 328, 528, 945, 1070
BsmFI GGGAC 4 cut(s) 692, 756, 1140, 1201
BsnI GGCC 5 cut(s) 482, 822, 1840, 1930, 2696
Bso31I GGTCTC 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 1237
Bsp1286I GDGCHC 3 cut(s) 1147, 1308, 1746
Bsp1720I GCTNAGC 1 cut(s) 2416
Bsp19I CCATGG 1 cut(s) 1505
BspACI CCGC 7 cut(s) 21, 144, 156, 823, 1677, 2196, 2634
BspANI GGCC 5 cut(s) 482, 822, 1840, 1930, 2696
BspCNI CTCAG 8 cut(s) 461, 487, 840, 2074, 2429, 2588, 2727, 2817
BspDI ATCGAT 2 cut(s) 993, 1958
BspFNI CGCG 1 cut(s) 1258
BspHI TCATGA 2 cut(s) 388, 2350
BspLI GGNNCC 6 cut(s) 142, 772, 1494, 1654, 2638, 2695
BspMAI CTGCAG 1 cut(s) 1642
BspMI ACCTGC 1 cut(s) 1805
BspPI GGATC 5 cut(s) 332, 396, 1273, 1339, 1963
BspT104I TTCGAA 1 cut(s) 1237
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 912
BsrFI RCCGGY 1 cut(s) 2192
BsrI ACTGG 8 cut(s) 712, 735, 1152, 1182, 1292, 2300, 2697, 2762
BssAI RCCGGY 1 cut(s) 2192
BssECI CCNNGG 4 cut(s) 773, 1398, 1505, 2943
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 2228
BssT1I CCWWGG 1 cut(s) 1505
Bst2BI CACGAG 1 cut(s) 2228
Bst2UI CCWGG 6 cut(s) 194, 775, 852, 1399, 1842, 2463
Bst4CI ACNGT 8 cut(s) 595, 924, 1373, 1447, 1618, 1825, 2009, 2726
Bst6I CTCTTC 2 cut(s) 1197, 1899
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 2 cut(s) 367, 1637
BstBI TTCGAA 1 cut(s) 1237
BstC8I GCNNGC 4 cut(s) 208, 372, 1256, 2306
BstDSI CCRYGG 1 cut(s) 1505
BstENI CCTNNNNNAGG 2 cut(s) 504, 2878
BstF5I GGATG 8 cut(s) 409, 641, 1247, 1394, 1870, 1912, 2269, 2330
BstFNI CGCG 1 cut(s) 1258
BstH2I RGCGCY 2 cut(s) 144, 448
BstHHI GCGC 2 cut(s) 143, 447
BstMAI GTCTC 5 cut(s) 183, 328, 528, 945, 1070
BstMWI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1278, 1637, 1676, 1760
BstNI CCWGG 6 cut(s) 194, 775, 852, 1399, 1842, 2463
BstNSI RCATGY 4 cut(s) 251, 435, 2749, 2920
BstSCI CCNGG 7 cut(s) 192, 773, 850, 1397, 1601, 1840, 2461
BstSFI CTRYAG 4 cut(s) 528, 1638, 1671, 2409
BstSLI GKGCMC 2 cut(s) 1147, 1308
BstUI CGCG 1 cut(s) 1258
BstV1I GCAGC 5 cut(s) 373, 1574, 1640, 1652, 2146
BstX2I RGATCY 2 cut(s) 401, 2185
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 2 cut(s) 401, 2185
Bsu15I ATCGAT 2 cut(s) 993, 1958
Bsu36I CCTNAGG 1 cut(s) 2575
BsuI GTATCC 1 cut(s) 2566
BsuRI GGCC 5 cut(s) 482, 822, 1840, 1930, 2696
BsuTUI ATCGAT 2 cut(s) 993, 1958
BtgI CCRYGG 1 cut(s) 1505
BtsCI GGATG 8 cut(s) 409, 641, 1247, 1394, 1870, 1912, 2269, 2330
BtsI GCAGTG 1 cut(s) 1731
BtsIMutI CAGTG 8 cut(s) 319, 694, 1189, 1443, 1614, 1731, 1821, 2704
BveI ACCTGC 1 cut(s) 1805
Cac8I GCNNGC 4 cut(s) 208, 372, 1256, 2306
CaiI CAGNNNCTG 3 cut(s) 367, 1835, 2874
CciI TCATGA 2 cut(s) 388, 2350
CfoI GCGC 2 cut(s) 143, 447
Cfr10I RCCGGY 1 cut(s) 2192
Cfr13I GGNCC 7 cut(s) 230, 755, 770, 1475, 1838, 2694, 2759
ClaI ATCGAT 2 cut(s) 993, 1958
CsiI ACCWGGT 2 cut(s) 192, 850
Csp6I GTAC 4 cut(s) 453, 979, 1697, 2765
CviQI GTAC 4 cut(s) 453, 979, 1697, 2765
DinI GGCGCC 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 1197, 1899
EarI CTCTTC 2 cut(s) 1197, 1899
EciI GGCGGA 1 cut(s) 2211
Eco130I CCWWGG 1 cut(s) 1505
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 5 cut(s) 230, 755, 770, 1475, 2759
Eco47III AGCGCT 1 cut(s) 446
Eco57I CTGAAG 3 cut(s) 1833, 2535, 2868
Eco81I CCTNAGG 1 cut(s) 2575
EcoNI CCTNNNNNAGG 2 cut(s) 504, 2878
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 6 cut(s) 192, 773, 850, 1397, 1840, 2461
EcoT14I CCWWGG 1 cut(s) 1505
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 1505
FalI AAGNNNNNCTT 6 cut(s) 210, 242, 1640, 1672, 2323, 2355
FaqI GGGAC 4 cut(s) 692, 756, 1140, 1201
FblI GTMKAC 1 cut(s) 2843
FokI GGATG 8 cut(s) 396, 628, 1234, 1381, 1877, 1919, 2256, 2337
FspBI CTAG 5 cut(s) 915, 1014, 1038, 1196, 1407
GlaI GCGC 2 cut(s) 142, 446
GsaI CCCAGC 1 cut(s) 1839
GsuI CTGGAG 1 cut(s) 1542
HaeII RGCGCY 2 cut(s) 144, 448
HaeIII GGCC 5 cut(s) 482, 822, 1840, 1930, 2696
HapII CCGG 4 cut(s) 126, 1602, 2193, 2777
HhaI GCGC 2 cut(s) 143, 447
Hin1I GRCGYC 1 cut(s) 141
Hin6I GCGC 2 cut(s) 141, 445
HinP1I GCGC 2 cut(s) 141, 445
HincII GTYRAC 2 cut(s) 2844, 2866
HindII GTYRAC 2 cut(s) 2844, 2866
HindIII AAGCTT 1 cut(s) 1279
HpaII CCGG 4 cut(s) 126, 1602, 2193, 2777
Hpy166II GTNNAC 9 cut(s) 317, 920, 1306, 1515, 2068, 2227, 2767, 2844, 2866
Hpy8I GTNNAC 9 cut(s) 317, 920, 1306, 1515, 2068, 2227, 2767, 2844, 2866
Hpy99I CGWCG 2 cut(s) 155, 173
HpyCH4III ACNGT 8 cut(s) 595, 924, 1373, 1447, 1618, 1825, 2009, 2726
HpyCH4IV ACGT 2 cut(s) 1334, 2064
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1278, 1637, 1676, 1760
HpySE526I ACGT 2 cut(s) 1334, 2064
Hsp92I GRCGYC 1 cut(s) 141
HspAI GCGC 2 cut(s) 141, 445
KasI GGCGCC 1 cut(s) 140
LmnI GCTCC 4 cut(s) 349, 1741, 1832, 2642
Lsp1109I GCAGC 5 cut(s) 373, 1574, 1640, 1652, 2146
MabI ACCWGGT 2 cut(s) 192, 850
MaeI CTAG 5 cut(s) 915, 1014, 1038, 1196, 1407
MaeII ACGT 2 cut(s) 1334, 2064
MfeI CAATTG 1 cut(s) 1386
MflI RGATCY 2 cut(s) 401, 2185
MhlI GDGCHC 3 cut(s) 1147, 1308, 1746
MlsI TGGCCA 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 482
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 3 cut(s) 1183, 2158, 2849
MmeI TCCRAC 3 cut(s) 819, 2482, 2833
Mox20I TGGCCA 1 cut(s) 482
MroXI GAANNNNTTC 5 cut(s) 86, 683, 1651, 1893, 2367
MscI TGGCCA 1 cut(s) 482
MseI TTAA 3 cut(s) 111, 942, 2627
MslI CAYNNNNRTG 1 cut(s) 1507
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 3 cut(s) 533, 1679, 2420
MspI CCGG 4 cut(s) 126, 1602, 2193, 2777
MspR9I CCNGG 7 cut(s) 194, 775, 852, 1399, 1603, 1842, 2463
MunI CAATTG 1 cut(s) 1386
MvaI CCWGG 6 cut(s) 194, 775, 852, 1399, 1842, 2463
MvnI CGCG 1 cut(s) 1258
MwoI GCNNNNNNNGC 7 cut(s) 152, 367, 902, 1278, 1637, 1676, 1760
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 1603
NcoI CCATGG 1 cut(s) 1505
NlaIV GGNNCC 6 cut(s) 142, 772, 1494, 1654, 2638, 2695
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 7 cut(s) 667, 1131, 1439, 1729, 1817, 2591, 2903
NspI RCATGY 4 cut(s) 251, 435, 2749, 2920
NspV TTCGAA 1 cut(s) 1237
OliI CACNNNNGTG 1 cut(s) 1507
PagI TCATGA 2 cut(s) 388, 2350
PaqCI CACCTGC 1 cut(s) 1805
PciI ACATGT 1 cut(s) 247
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 554
PdmI GAANNNNTTC 5 cut(s) 86, 683, 1651, 1893, 2367
PfeI GAWTC 9 cut(s) 173, 396, 582, 911, 1889, 2107, 2345, 2771, 2816
PflMI CCANNNNNTGG 2 cut(s) 881, 2435
PleI GAGTC 3 cut(s) 1183, 2157, 2848
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 3 cut(s) 1183, 2157, 2848
PscI ACATGT 1 cut(s) 247
PsiI TTATAA 1 cut(s) 1103
Psp6I CCWGG 6 cut(s) 192, 773, 850, 1397, 1840, 2461
PspFI CCCAGC 1 cut(s) 1835
PspGI CCWGG 6 cut(s) 192, 773, 850, 1397, 1840, 2461
PspN4I GGNNCC 6 cut(s) 142, 772, 1494, 1654, 2638, 2695
PspPI GGNCC 7 cut(s) 230, 755, 770, 1475, 1838, 2694, 2759
PstI CTGCAG 1 cut(s) 1642
PstNI CAGNNNCTG 3 cut(s) 367, 1835, 2874
PsuI RGATCY 2 cut(s) 401, 2185
PvuII CAGCTG 2 cut(s) 533, 2420
RsaI GTAC 4 cut(s) 454, 980, 1698, 2766
RsaNI GTAC 4 cut(s) 453, 979, 1697, 2765
RseI CAYNNNNRTG 1 cut(s) 1507
SalI GTCGAC 1 cut(s) 2842
SaqAI TTAA 3 cut(s) 111, 942, 2627
Sau96I GGNCC 7 cut(s) 230, 755, 770, 1475, 1838, 2694, 2759
ScaI AGTACT 1 cut(s) 980
SchI GAGTC 3 cut(s) 1183, 2158, 2849
ScrFI CCNGG 7 cut(s) 194, 775, 852, 1399, 1603, 1842, 2463
SduI GDGCHC 3 cut(s) 1147, 1308, 1746
SexAI ACCWGGT 2 cut(s) 192, 850
SfcI CTRYAG 4 cut(s) 528, 1638, 1671, 2409
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 1237
SinI GGWCC 5 cut(s) 230, 755, 770, 1475, 2759
SmiMI CAYNNNNRTG 1 cut(s) 1507
SmlI CTYRAG 5 cut(s) 325, 618, 674, 1967, 2329
SmoI CTYRAG 5 cut(s) 325, 618, 674, 1967, 2329
SsiI CCGC 7 cut(s) 21, 144, 156, 823, 1677, 2196, 2634
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 2545
SspMI CTAG 5 cut(s) 915, 1014, 1038, 1196, 1407
StyD4I CCNGG 7 cut(s) 192, 773, 850, 1397, 1601, 1840, 2461
StyI CCWWGG 1 cut(s) 1505
TaaI ACNGT 8 cut(s) 595, 924, 1373, 1447, 1618, 1825, 2009, 2726
TaiI ACGT 2 cut(s) 1337, 2067
TatI WGTACW 2 cut(s) 978, 2764
TauI GCSGC 6 cut(s) 24, 146, 158, 825, 1679, 2637
TfiI GAWTC 9 cut(s) 173, 396, 582, 911, 1889, 2107, 2345, 2771, 2816
Tru1I TTAA 3 cut(s) 111, 942, 2627
Tru9I TTAA 3 cut(s) 111, 942, 2627
TscAI CASTG 8 cut(s) 319, 694, 1189, 1450, 1621, 1738, 1828, 2704
TseFI GTSAC 7 cut(s) 667, 1131, 1439, 1729, 1817, 2591, 2903
TseI GCWGC 5 cut(s) 361, 1562, 1628, 1640, 2159
Tsp45I GTSAC 7 cut(s) 667, 1131, 1439, 1729, 1817, 2591, 2903
TspGWI ACGGA 6 cut(s) 139, 157, 575, 1488, 1684, 2142
TspRI CASTG 8 cut(s) 319, 694, 1189, 1450, 1621, 1738, 1828, 2704
Van91I CCANNNNNTGG 2 cut(s) 881, 2435
VneI GTGCAC 1 cut(s) 1304
VpaK11BI GGWCC 5 cut(s) 230, 755, 770, 1475, 2759
XagI CCTNNNNNAGG 2 cut(s) 504, 2878
XapI RAATTY 7 cut(s) 82, 92, 683, 1033, 2173, 2377, 2398
XceI RCATGY 4 cut(s) 251, 435, 2749, 2920
XcmI CCANNNNNNNNNTGG 2 cut(s) 1503, 2465
XmiI GTMKAC 1 cut(s) 2843
XmnI GAANNNNTTC 5 cut(s) 86, 683, 1651, 1893, 2367
XspI CTAG 5 cut(s) 915, 1014, 1038, 1196, 1407
ZrmI AGTACT 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.