Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0007016

Physical Location & Seq

Genomic Coordinates Forward (+)

30110907 .. 30120475

Gene Structure 9569 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1219200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGGCGTTCCTGCGTTTTACCGCTGGCTAGCGGATCGGTACCCGCAGTCCATCGCCGACGTCGTGGAGGAGCATGCCAGAGAGGACTCCCATGGCGTCCCTGTGCCAGTCGACGTCTCCGGACCGAACCCTAATGGGTCCGAGTTCGACAACTTGTACCTTGACATGAACGGCATTATACATCCCTGTTTTCATCCCGATGGGAAGTCGCCTGCCCCTGCTACCTATGATGATGTATTCAAATCAATATTTGATTACATTGATCATCTATTCACATTGGTTCGCCCAAGAAAGCTTCTTTTTTTGGCAATTGATGGGGTTGCTCCAAGAGCCAAAATGAATCAGCAACGTTCTCGACGTTACAGAGCTTCAAAAGATGCGGCTGAAGCGGAAGCTGAAGAGGAAAGACTTAAGAAAGAATTTGAGATGGAGGGGACATCTTTGGCTTCTAAAGAGAAACCTGAAACTTTAGACTCAAATGTCATAACCCCGGGCACTCAATTTATGGCAGTGCTCTCGGTGGCACTTCAATATTACATACATAGTAGGTTGAACCACAACCCTGGTTGGCGGTCTACGAAGGTTATACTTTCTGATGCAAATGTTCCTGGCGAGGGAGAGCACAAAATAATGTCATACATCCGATTGCAACGCAACCTCCCTGGTTTTGATCCTAACACTCGACACTGTCTTTATGGTTTGGATGCAGATCTAATTATGCTGGCTTTAGCTACACATGAGGTTCACTTCTCAATATTAAGAGAGGTGATTACTTTTCCAGGTCAACAAGAAAAATGTTTTCTTTGTGGACAAGTTGGTCATCTGGCAGCTGAGTGTCGTGGTATATCTGATGATAATGGAAACGTGGTTGATGATACGCCAATTCACCAGAAGAAATATCAGTTCCTCAACATATGGGTATTGCGTGAATATTTGCAATATGAGCTGGATATTCCCAACCCTCCTTTTCAGCTCAATTTTGAAAGGATAGTGGATGATTTTGTTTTTATGTGCTTTTTTGTTGGCAATGACTTTCTTCCACATATGCCAACATTGGAGATCAGAGAGGGTGCTATTAATTTATTGATGCATATTTATAGAACGGAGTTCACTGCTATGGGAGGTTATCTTACTGATGCGGGTGAGGTATTGCTAGACAGAGTAGAGCATTTTATTCAGTCTGTTGCTGTTTTTGAAAGCCAAATCTTTCAAAAGCGGGTTCGAATTCAGCAGGCTATTGAGAATAATGAAGAGAGACATAGAGCTAGAAGAGAAACTTCAGCAGAGCTACCGGCTCCGGTGATGGACAAGGTTAAATTGGGAGAGCCTGGATATACAGAAAGGTATTATGCTGAGAAATTTCAAGTATCAACACCAGAAGAGATTGATAAAGTTAAAAAGGACTTGGTTTTAAAATATGTAGAAGGCTTATGTTGGGTTTGCCGATATTATTACCAAGGAGTCTGCTCATGGATATGGTATTATCCATACCATTATGCTCCTTTTGCTTCTGATCTAAAAGATTTAAATGAACTGGAAATAACATTTTTCTTGGGAGAGCCATTTAAGCCCTTCGATCAGCTAATGGGAACCCTACCAGCTGCAAGCTCTAGTGCTCTTCCGGAAAAATACAGAAACTTAATGACTGATCCAAGATCACCTATCCATAATTTCTATCCTGCTGATTTTGAAATTGACATGAATGGCAAGCGCTTTGCATGGCAGGGTGTTGCCAAATTGCCGTTCATTAACGAGAGGAAATTGGTTACTGAGACAAGGAAGCTTGAAAGCACTTTAACGGAAGAAGAGCAGGCTCGGAATAGCATGATGCTTGATTTGCTTTATGTTTATCCTTCTCATCCTTTGGCAGCACAAATCAGTATGTACTACCAACAGTTTTATCAGACACCCCTACATGAAAGATTTTTATGGCGGGTAGACCCAATTGCTAGTGGCGGAATGAATGGATTCCTTGGGTTATCTGAGAGAAATGGCTTAAGATATGTAATCCCTTCCCCTATTCGTGGTTATCCTGACCTTGATTGTAACCAAGTCTTAAATATTACATATCTTAATCCGGCAGATCATAAACACATCCCAGAACCTCCCAAGGGTGTGATCCTGCCCAGAAAGGTTGTAAGACCTACGGACATCAAACCATTCCCTACGTTATGGCACGACGAAGGTCCTCGACGCCAAGGCAGAGAGAGGCCACAAATACCTGGAGCAATTGCTGGACCCATGTTGGGAGAAGCAGCTCACCGCCTGGTCAAGAACACCCTTAATATTAAACCTAATGGTTCATCCCCTGGATTATGGGATCAACCGCCATTACGAAATTCCGGAAATTATGCAGGAAATTATCCAGTCAACAGACCAAGGCCAGCTGGGCCCTCTGGGTATGAAAGGGTCTTCCGCGAAGAAGCAACGTATGGAAATTCCTTCAATCCTCAGGTCATGATGGCTAGGCCTAGAATTCCTGCATCAAATGGTATGCAAGGTGACAGACAGAATTTTAGGGCGCAAGAAAGAGTACAACATCAAGAACAGAATTTTAGGACACAAGATAGAGTACAAGATCAAGAACAGTACAACCTGAGATCCGTGATGTCGAGTTTAACAGTGCAAGACAGTGGGAGAACCAGGTCACCTGCAGCGGCGCCACCTGGGATGCCAAATTCAGGATACCAAGCAAACCGTAAGCACCAGTTTGTGCAGAACATGGGTGCTCTTCCTTCGCCACCTACCAAGTGGATTAGCAAAGAACCAACTGGAAATGGTGGATTACATGTAAGGAAACAGGAAACTGGATATGGTGGAGCATATGAGCAACAGCAGGTCAAGAAGGTCTATCAAGTTAAGACGCGGGCTCCCCAGGATGTCAAACCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000291 GO:0000902 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009826 GO:0009888 GO:0009892 GO:0009987 GO:0010087 GO:0010286 GO:0010468 GO:0010494 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016049 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0031086 GO:0031087 GO:0032502 GO:0032989 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0040007 GO:0040029 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051301 GO:0051716 GO:0060255 GO:0060560 GO:0065007 GO:0070370 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 ko04016 map03008 map03018 map04016

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

964

Amino Acids

110.09

Weight (kDa)

6.57

Isoelectric Point (pI)

47.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 255	4.7e-100	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	325 - 427	2.4e-42	Xrn1 helical domain
XRN_M	PF17846	435 - 718	5.6e-111	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54490 AT1G54490
fragaria_vesca	FvH4_6g03690 FvH4_6g03690
malus_domestica	MD04G1219200.v1.1 MD12G1236200.v1.1
prunus_persica	Prupe.6G338500_v2.0.a1 Prupe.6G338500_v2.0.a1
pyrus_communis	pycom04g19450 pycom12g21730
rosa_chinensis	RchiOBHm_Chr3g0452231
rosa_laevigata	RLG00000025615
rosa_multiflora	Rmu_sc0037132.1_g000001 Rmu_sc0041263.1_g000001
rosa_roxburghii	Rroxscaffold_164G00435930 Rroxscaffold_6G00429350
rosa_rugosa	Rorug02G0639400
rosa_samantha	Rh3AG041300 Rh3BG043000 Rh3BG043200 Rh3CG041400 Rh3CG041600 Rh3DG042300 Rh3DG042500
rosa_wichuraiana	Rw3G003160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2659
AasI	GACNNNNNNGTC	2 cut(s)	479, 816
AatII	GACGTC	2 cut(s)	63, 117
Acc36I	ACCTGC	2 cut(s)	2659, 2827
Acc65I	GGTACC	1 cut(s)	39
AccB1I	GGYRCC	2 cut(s)	39, 2659
AccI	GTMKAC	3 cut(s)	111, 575, 1938
AccII	CGCG	2 cut(s)	2418, 2866
AccIII	TCCGGA	3 cut(s)	119, 1621, 2342
AclI	AACGTT	1 cut(s)	349
AclWI	GGATC	6 cut(s)	42, 665, 1643, 2113, 2328, 2595
AcsI	RAATTY	9 cut(s)	419, 1224, 1358, 2338, 2437, 2475, 2512, 2551, 2677
AcuI	CTGAAG	3 cut(s)	405, 417, 1263
AcyI	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
AfaI	GTAC	6 cut(s)	41, 158, 1886, 2535, 2574, 2591
AfeI	AGCGCT	1 cut(s)	1712
AfiI	CCNNNNNNNGG	5 cut(s)	614, 2024, 2111, 2246, 2681
AflII	CTTAAG	2 cut(s)	410, 1996
AflIII	ACRYGT	1 cut(s)	2788
AjuI	GAANNNNNNNTTGG	2 cut(s)	1727, 1759
Alw21I	GWGCWC	4 cut(s)	516, 624, 1618, 2731
Alw26I	GTCTC	3 cut(s)	121, 1249, 1766
AlwI	GGATC	6 cut(s)	42, 665, 1643, 2113, 2328, 2595
Ama87I	CYCGRG	1 cut(s)	490
Aor13HI	TCCGGA	3 cut(s)	119, 1621, 2342
Aor51HI	AGCGCT	1 cut(s)	1712
AoxI	GGCC	4 cut(s)	2210, 2381, 2390, 2468
ApaI	GGGCCC	1 cut(s)	2394
ApeKI	GCWGC	5 cut(s)	827, 1601, 1868, 2255, 2654
ApoI	RAATTY	9 cut(s)	419, 1224, 1358, 2338, 2437, 2475, 2512, 2551, 2677
AseI	ATTAAT	1 cut(s)	1077
Asp700I	GAANNNNTTC	1 cut(s)	1967
Asp718I	GGTACC	1 cut(s)	39
AspLEI	GCGC	3 cut(s)	1713, 2524, 2662
AspS9I	GGNCC	6 cut(s)	122, 138, 2186, 2237, 2390, 2391
AsuC2I	CCSGG	2 cut(s)	491, 492
AsuHPI	GGTGA	8 cut(s)	778, 878, 1154, 1312, 1650, 2252, 2513, 2640
AsuII	TTCGAA	1 cut(s)	1222
AsuNHI	GCTAGC	1 cut(s)	28
AvaI	CYCGRG	1 cut(s)	490
AvaII	GGWCC	4 cut(s)	122, 138, 2186, 2237
AxyI	CCTNAGG	1 cut(s)	2451
BaeGI	GKGCMC	2 cut(s)	497, 2394
BanI	GGYRCC	2 cut(s)	39, 2659
BanII	GRGCYC	2 cut(s)	2394, 2872
BbsI	GAAGAC	1 cut(s)	2404
Bbv12I	GWGCWC	4 cut(s)	516, 624, 1618, 2731
BbvI	GCAGC	5 cut(s)	839, 1588, 1880, 2267, 2666
BccI	CCATC	6 cut(s)	59, 194, 308, 421, 1297, 2455
BceAI	ACGGC	2 cut(s)	187, 1726
BcgI	CGANNNNNNTGC	4 cut(s)	335, 369, 498, 532
BciVI	GTATCC	1 cut(s)	2678
BclI	TGATCA	1 cut(s)	262
BcnI	CCSGG	2 cut(s)	491, 492
BcoDI	GTCTC	3 cut(s)	121, 1249, 1766
BfaI	CTAG	7 cut(s)	29, 1154, 1266, 1611, 1950, 2466, 2472
BfmI	CTRYAG	1 cut(s)	2652
BfoI	RGCGCY	2 cut(s)	1714, 2663
BfrI	CTTAAG	2 cut(s)	410, 1996
BfuAI	ACCTGC	2 cut(s)	2659, 2827
BfuI	GTATCC	1 cut(s)	2678
BglI	GCCNNNNNGGC	1 cut(s)	2389
BglII	AGATCT	1 cut(s)	709
BisI	GCNGC	7 cut(s)	381, 828, 1602, 1869, 2256, 2655, 2658
BlsI	GCNGC	7 cut(s)	382, 829, 1603, 1870, 2257, 2656, 2659
Bme18I	GGWCC	4 cut(s)	122, 138, 2186, 2237
BmeT110I	CYCGRG	1 cut(s)	490
BmgT120I	GGNCC	6 cut(s)	122, 138, 2186, 2237, 2390, 2391
BmiI	GGNNCC	8 cut(s)	41, 139, 1296, 1591, 2239, 2392, 2661, 2871
BmsI	GCATC	8 cut(s)	367, 586, 694, 1077, 1126, 1818, 2492, 2661
BmtI	GCTAGC	1 cut(s)	32
BoxI	GACNNNNGTC	1 cut(s)	2184
BpiI	GAAGAC	1 cut(s)	2404
BpmI	CTGGAG	1 cut(s)	2244
Bpu14I	TTCGAA	1 cut(s)	1222
BpuMI	CCSGG	2 cut(s)	491, 492
BsaBI	GATNNNNATC	1 cut(s)	708
BsaHI	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
BsaWI	WCCGGW	4 cut(s)	119, 1297, 1621, 2342
BsaXI	ACNNNNNCTCC	4 cut(s)	642, 672, 2854, 2884
Bsc4I	CCNNNNNNNGG	5 cut(s)	614, 2024, 2111, 2246, 2681
Bse118I	RCCGGY	1 cut(s)	1291
Bse1I	ACTGG	6 cut(s)	107, 1539, 2366, 2707, 2776, 2812
Bse21I	CCTNAGG	1 cut(s)	2451
Bse3DI	GCAATG	1 cut(s)	1033
Bse8I	GATNNNNATC	1 cut(s)	708
BseAI	TCCGGA	3 cut(s)	119, 1621, 2342
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	5 cut(s)	614, 2024, 2111, 2246, 2681
BseMI	GCAATG	1 cut(s)	1033
BseMII	CTCAG	6 cut(s)	822, 1344, 1761, 1974, 2465, 2588
BseNI	ACTGG	6 cut(s)	107, 1539, 2366, 2707, 2776, 2812
BseRI	GAGGAG	1 cut(s)	83
BseSI	GKGCMC	2 cut(s)	497, 2394
BseXI	GCAGC	5 cut(s)	839, 1588, 1880, 2267, 2666
BseYI	CCCAGC	1 cut(s)	2387
BsgI	GTGCAG	1 cut(s)	2735
Bsh1236I	CGCG	2 cut(s)	2418, 2866
BshFI	GGCC	4 cut(s)	2212, 2383, 2392, 2470
BshNI	GGYRCC	2 cut(s)	39, 2659
BsiHKAI	GWGCWC	4 cut(s)	516, 624, 1618, 2731
BsiHKCI	CYCGRG	1 cut(s)	490
BsiSI	CCGG	7 cut(s)	120, 491, 1292, 1298, 1622, 2078, 2343
BslFI	GGGAC	2 cut(s)	83, 448
BslI	CCNNNNNNNGG	5 cut(s)	614, 2024, 2111, 2246, 2681
BsmAI	GTCTC	3 cut(s)	121, 1249, 1766
BsmBI	CGTCTC	1 cut(s)	121
BsmFI	GGGAC	2 cut(s)	83, 448
BsnI	GGCC	4 cut(s)	2212, 2383, 2392, 2470
BsoBI	CYCGRG	1 cut(s)	490
Bsp119I	TTCGAA	1 cut(s)	1222
Bsp120I	GGGCCC	1 cut(s)	2390
Bsp1286I	GDGCHC	7 cut(s)	497, 516, 624, 1618, 2394, 2731, 2872
Bsp13I	TCCGGA	3 cut(s)	119, 1621, 2342
Bsp19I	CCATGG	1 cut(s)	91
BspANI	GGCC	4 cut(s)	2212, 2383, 2392, 2470
BspCNI	CTCAG	6 cut(s)	823, 1345, 1762, 1975, 2464, 2589
BspEI	TCCGGA	3 cut(s)	119, 1621, 2342
BspFNI	CGCG	2 cut(s)	2418, 2866
BspHI	TCATGA	1 cut(s)	2457
BspLI	GGNNCC	8 cut(s)	41, 139, 1296, 1591, 2239, 2392, 2661, 2871
BspMAI	CTGCAG	1 cut(s)	2656
BspMI	ACCTGC	2 cut(s)	2659, 2827
BspOI	GCTAGC	1 cut(s)	32
BspPI	GGATC	6 cut(s)	42, 665, 1643, 2113, 2328, 2595
BspQI	GCTCTTC	3 cut(s)	1623, 1800, 2736
BspT104I	TTCGAA	1 cut(s)	1222
BspT107I	GGYRCC	2 cut(s)	39, 2659
BspTI	CTTAAG	2 cut(s)	410, 1996
BsrDI	GCAATG	1 cut(s)	1033
BsrFI	RCCGGY	1 cut(s)	1291
BsrI	ACTGG	6 cut(s)	107, 1539, 2366, 2707, 2776, 2812
BssAI	RCCGGY	1 cut(s)	1291
BssNI	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
BssT1I	CCWWGG	6 cut(s)	91, 1456, 1972, 2109, 2197, 2378
Bst4CI	ACNGT	7 cut(s)	689, 1896, 2589, 2623, 2633, 2699, 2889
Bst6I	CTCTTC	7 cut(s)	393, 1245, 1264, 1374, 1623, 1800, 2736
BstACI	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
BstAFI	CTTAAG	2 cut(s)	410, 1996
BstBI	TTCGAA	1 cut(s)	1222
BstDEI	CTNAG	6 cut(s)	831, 1353, 1770, 1983, 2451, 2597
BstDSI	CCRYGG	1 cut(s)	91
BstEII	GGTNACC	1 cut(s)	2646
BstFNI	CGCG	2 cut(s)	2418, 2866
BstH2I	RGCGCY	2 cut(s)	1714, 2663
BstHHI	GCGC	3 cut(s)	1713, 2524, 2662
BstMAI	GTCTC	3 cut(s)	121, 1249, 1766
BstMWI	GCNNNNNNNGC	7 cut(s)	329, 386, 943, 1505, 1567, 1837, 2389
BstNSI	RCATGY	2 cut(s)	77, 2792
BstPAI	GACNNNNGTC	1 cut(s)	2184
BstPI	GGTNACC	1 cut(s)	2646
BstSFI	CTRYAG	1 cut(s)	2652
BstSLI	GKGCMC	2 cut(s)	497, 2394
BstUI	CGCG	2 cut(s)	2418, 2866
BstV1I	GCAGC	5 cut(s)	839, 1588, 1880, 2267, 2666
BstV2I	GAAGAC	1 cut(s)	2404
BstX2I	RGATCY	2 cut(s)	709, 2600
BstXI	CCANNNNNNTGG	1 cut(s)	563
BstYI	RGATCY	2 cut(s)	709, 2600
Bsu36I	CCTNAGG	1 cut(s)	2451
BsuI	GTATCC	1 cut(s)	2678
BsuRI	GGCC	4 cut(s)	2212, 2383, 2392, 2470
BtgI	CCRYGG	1 cut(s)	91
BtgZI	GCGATG	1 cut(s)	37
BtsI	GCAGTG	2 cut(s)	516, 1110
BtsIMutI	CAGTG	5 cut(s)	516, 685, 1110, 2628, 2638
BveI	ACCTGC	2 cut(s)	2659, 2827
CciI	TCATGA	1 cut(s)	2457
CfoI	GCGC	3 cut(s)	1713, 2524, 2662
Cfr10I	RCCGGY	1 cut(s)	1291
Cfr13I	GGNCC	6 cut(s)	122, 138, 2186, 2237, 2390, 2391
Cfr9I	CCCGGG	1 cut(s)	490
CpoI	CGGWCCG	1 cut(s)	122
CseI	GACGC	3 cut(s)	85, 2202, 2872
CsiI	ACCWGGT	1 cut(s)	2642
Csp6I	GTAC	6 cut(s)	40, 157, 1885, 2534, 2573, 2590
CspI	CGGWCCG	1 cut(s)	122
CviQI	GTAC	6 cut(s)	40, 157, 1885, 2534, 2573, 2590
DdeI	CTNAG	6 cut(s)	831, 1353, 1770, 1983, 2451, 2597
DinI	GGCGCC	1 cut(s)	2661
DraI	TTTAAA	2 cut(s)	1413, 1527
DrdI	GACNNNNNNGTC	2 cut(s)	479, 816
DseDI	GACNNNNNNGTC	2 cut(s)	479, 816
Eam1104I	CTCTTC	7 cut(s)	393, 1245, 1264, 1374, 1623, 1800, 2736
EarI	CTCTTC	7 cut(s)	393, 1245, 1264, 1374, 1623, 1800, 2736
EciI	GGCGGA	1 cut(s)	1971
Eco130I	CCWWGG	6 cut(s)	91, 1456, 1972, 2109, 2197, 2378
Eco147I	AGGCCT	1 cut(s)	2470
Eco24I	GRGCYC	2 cut(s)	2394, 2872
Eco47I	GGWCC	4 cut(s)	122, 138, 2186, 2237
Eco47III	AGCGCT	1 cut(s)	1712
Eco57I	CTGAAG	3 cut(s)	405, 417, 1263
Eco81I	CCTNAGG	1 cut(s)	2451
Eco88I	CYCGRG	1 cut(s)	490
Eco91I	GGTNACC	1 cut(s)	2646
EcoO109I	RGGNCCY	2 cut(s)	2186, 2391
EcoO65I	GGTNACC	1 cut(s)	2646
EcoRI	GAATTC	2 cut(s)	1224, 2475
EcoT14I	CCWWGG	6 cut(s)	91, 1456, 1972, 2109, 2197, 2378
EcoT22I	ATGCAT	1 cut(s)	1092
EcoT38I	GRGCYC	2 cut(s)	2394, 2872
EgeI	GGCGCC	1 cut(s)	2661
EheI	GGCGCC	1 cut(s)	2661
ErhI	CCWWGG	6 cut(s)	91, 1456, 1972, 2109, 2197, 2378
Esp3I	CGTCTC	1 cut(s)	121
FalI	AAGNNNNNCTT	2 cut(s)	1261, 1293
FaqI	GGGAC	2 cut(s)	83, 448
FauI	CCCGC	5 cut(s)	51, 1132, 1209, 1926, 2859
FauNDI	CATATG	3 cut(s)	914, 1044, 2824
FbaI	TGATCA	1 cut(s)	262
FblI	GTMKAC	3 cut(s)	111, 575, 1938
Fnu4HI	GCNGC	7 cut(s)	381, 828, 1602, 1869, 2256, 2655, 2658
FokI	GGATG	9 cut(s)	168, 180, 626, 716, 1007, 1845, 2081, 2290, 2683
FriOI	GRGCYC	2 cut(s)	2394, 2872
Fsp4HI	GCNGC	7 cut(s)	381, 828, 1602, 1869, 2256, 2655, 2658
FspBI	CTAG	7 cut(s)	29, 1154, 1266, 1611, 1950, 2466, 2472
GlaI	GCGC	3 cut(s)	1712, 2523, 2661
GluI	GCNGC	7 cut(s)	381, 828, 1602, 1869, 2256, 2655, 2658
GsaI	CCCAGC	1 cut(s)	2391
GsuI	CTGGAG	1 cut(s)	2244
HaeII	RGCGCY	2 cut(s)	1714, 2663
HaeIII	GGCC	4 cut(s)	2212, 2383, 2392, 2470
HapII	CCGG	7 cut(s)	120, 491, 1292, 1298, 1622, 2078, 2343
HgaI	GACGC	3 cut(s)	85, 2202, 2872
HhaI	GCGC	3 cut(s)	1713, 2524, 2662
Hin1I	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
Hin6I	GCGC	3 cut(s)	1711, 2522, 2660
HinP1I	GCGC	3 cut(s)	1711, 2522, 2660
HincII	GTYRAC	3 cut(s)	112, 785, 2371
HindII	GTYRAC	3 cut(s)	112, 785, 2371
HindIII	AAGCTT	2 cut(s)	293, 1781
HinfI	GANTC	5 cut(s)	86, 340, 473, 1461, 1968
HpaII	CCGG	7 cut(s)	120, 491, 1292, 1298, 1622, 2078, 2343
HphI	GGTGA	8 cut(s)	778, 878, 1154, 1312, 1650, 2252, 2513, 2640
Hpy166II	GTNNAC	8 cut(s)	112, 576, 745, 785, 809, 1110, 1939, 2371
Hpy188I	TCNGA	9 cut(s)	142, 595, 644, 850, 1064, 1513, 1818, 1905, 1984
Hpy8I	GTNNAC	8 cut(s)	112, 576, 745, 785, 809, 1110, 1939, 2371
Hpy99I	CGWCG	6 cut(s)	62, 65, 116, 360, 2183, 2196
HpyAV	CCTTC	9 cut(s)	574, 1418, 1582, 1863, 2022, 2177, 2452, 2745, 2839
HpyCH4III	ACNGT	7 cut(s)	689, 1896, 2589, 2623, 2633, 2699, 2889
HpyCH4IV	ACGT	7 cut(s)	60, 114, 349, 358, 864, 2168, 2429
HpyF10VI	GCNNNNNNNGC	7 cut(s)	329, 386, 943, 1505, 1567, 1837, 2389
HpyF3I	CTNAG	6 cut(s)	831, 1353, 1770, 1983, 2451, 2597
HpySE526I	ACGT	7 cut(s)	60, 114, 349, 358, 864, 2168, 2429
Hsp92I	GRCGYC	5 cut(s)	60, 96, 114, 2194, 2660
HspAI	GCGC	3 cut(s)	1711, 2522, 2660
KasI	GGCGCC	1 cut(s)	2659
Kpn2I	TCCGGA	3 cut(s)	119, 1621, 2342
KpnI	GGTACC	1 cut(s)	43
Ksp22I	TGATCA	1 cut(s)	262
LguI	GCTCTTC	3 cut(s)	1623, 1800, 2736
LmnI	GCTCC	7 cut(s)	70, 328, 1300, 1504, 2225, 2819, 2875
Lsp1109I	GCAGC	5 cut(s)	839, 1588, 1880, 2267, 2666
LweI	GCATC	8 cut(s)	367, 586, 694, 1077, 1126, 1818, 2492, 2661
MabI	ACCWGGT	1 cut(s)	2642
MaeI	CTAG	7 cut(s)	29, 1154, 1266, 1611, 1950, 2466, 2472
MaeII	ACGT	7 cut(s)	60, 114, 349, 358, 864, 2168, 2429
MaeIII	GTNAC	5 cut(s)	359, 1765, 2045, 2501, 2646
MfeI	CAATTG	3 cut(s)	309, 1944, 2229
MflI	RGATCY	2 cut(s)	709, 2600
MhlI	GDGCHC	7 cut(s)	497, 516, 624, 1618, 2394, 2731, 2872
Mly113I	GGCGCC	1 cut(s)	2660
MlyI	GAGTC	3 cut(s)	80, 467, 1470
Mph1103I	ATGCAT	1 cut(s)	1092
MroI	TCCGGA	3 cut(s)	119, 1621, 2342
MroXI	GAANNNNTTC	1 cut(s)	1967
MslI	CAYNNNNRTG	3 cut(s)	198, 1115, 1474
MspA1I	CMGCKG	5 cut(s)	24, 830, 1601, 2387, 2657
MspCI	CTTAAG	2 cut(s)	410, 1996
MspI	CCGG	7 cut(s)	120, 491, 1292, 1298, 1622, 2078, 2343
MunI	CAATTG	3 cut(s)	309, 1944, 2229
MvnI	CGCG	2 cut(s)	2418, 2866
MwoI	GCNNNNNNNGC	7 cut(s)	329, 386, 943, 1505, 1567, 1837, 2389
NarI	GGCGCC	1 cut(s)	2660
NciI	CCSGG	2 cut(s)	491, 492
NcoI	CCATGG	1 cut(s)	91
NdeI	CATATG	3 cut(s)	914, 1044, 2824
NheI	GCTAGC	1 cut(s)	28
NlaIV	GGNNCC	8 cut(s)	41, 139, 1296, 1591, 2239, 2392, 2661, 2871
NmuCI	GTSAC	2 cut(s)	2501, 2646
NsiI	ATGCAT	1 cut(s)	1092
NspI	RCATGY	2 cut(s)	77, 2792
NspV	TTCGAA	1 cut(s)	1222
PaeI	GCATGC	1 cut(s)	77
PagI	TCATGA	1 cut(s)	2457
PaqCI	CACCTGC	1 cut(s)	2659
PceI	AGGCCT	1 cut(s)	2470
PciI	ACATGT	1 cut(s)	2788
PciSI	GCTCTTC	3 cut(s)	1623, 1800, 2736
PcsI	WCGNNNNNNNCGW	2 cut(s)	60, 355
PdmI	GAANNNNTTC	1 cut(s)	1967
PfeI	GAWTC	2 cut(s)	340, 1968
PflFI	GACNNNGTC	1 cut(s)	687
PkrI	GCNGC	7 cut(s)	382, 829, 1603, 1870, 2257, 2656, 2659
PleI	GAGTC	3 cut(s)	80, 467, 1469
PluTI	GGCGCC	1 cut(s)	2663
PpsI	GAGTC	3 cut(s)	80, 467, 1469
PpuMI	RGGWCCY	1 cut(s)	2186
PscI	ACATGT	1 cut(s)	2788
PshAI	GACNNNNGTC	1 cut(s)	2184
PshBI	ATTAAT	1 cut(s)	1077
Psp1406I	AACGTT	1 cut(s)	349
Psp5II	RGGWCCY	1 cut(s)	2186
PspEI	GGTNACC	1 cut(s)	2646
PspFI	CCCAGC	1 cut(s)	2387
PspN4I	GGNNCC	8 cut(s)	41, 139, 1296, 1591, 2239, 2392, 2661, 2871
PspOMI	GGGCCC	1 cut(s)	2390
PspPI	GGNCC	6 cut(s)	122, 138, 2186, 2237, 2390, 2391
PspPPI	RGGWCCY	1 cut(s)	2186
PstI	CTGCAG	1 cut(s)	2656
PsuI	RGATCY	2 cut(s)	709, 2600
PsyI	GACNNNGTC	1 cut(s)	687
PvuII	CAGCTG	3 cut(s)	830, 1601, 2387
RsaI	GTAC	6 cut(s)	41, 158, 1886, 2535, 2574, 2591
RsaNI	GTAC	6 cut(s)	40, 157, 1885, 2534, 2573, 2590
RseI	CAYNNNNRTG	3 cut(s)	198, 1115, 1474
Rsr2I	CGGWCCG	1 cut(s)	122
RsrII	CGGWCCG	1 cut(s)	122
SalI	GTCGAC	1 cut(s)	110
SapI	GCTCTTC	3 cut(s)	1623, 1800, 2736
SatI	GCNGC	7 cut(s)	381, 828, 1602, 1869, 2256, 2655, 2658
Sau96I	GGNCC	6 cut(s)	122, 138, 2186, 2237, 2390, 2391
SchI	GAGTC	3 cut(s)	80, 467, 1470
SduI	GDGCHC	7 cut(s)	497, 516, 624, 1618, 2394, 2731, 2872
SexAI	ACCWGGT	1 cut(s)	2642
SfaNI	GCATC	8 cut(s)	367, 586, 694, 1077, 1126, 1818, 2492, 2661
SfcI	CTRYAG	1 cut(s)	2652
SfiI	GGCCNNNNNGGCC	1 cut(s)	2389
SfoI	GGCGCC	1 cut(s)	2661
SfuI	TTCGAA	1 cut(s)	1222
SinI	GGWCC	4 cut(s)	122, 138, 2186, 2237
SmaI	CCCGGG	1 cut(s)	492
SmiI	ATTTAAAT	1 cut(s)	1527
SmiMI	CAYNNNNRTG	3 cut(s)	198, 1115, 1474
SmlI	CTYRAG	2 cut(s)	410, 1996
SmoI	CTYRAG	2 cut(s)	410, 1996
SphI	GCATGC	1 cut(s)	77
SseBI	AGGCCT	1 cut(s)	2470
SspDI	GGCGCC	1 cut(s)	2659
SspI	AATATT	6 cut(s)	249, 533, 756, 932, 2062, 2287
SspMI	CTAG	7 cut(s)	29, 1154, 1266, 1611, 1950, 2466, 2472
StuI	AGGCCT	1 cut(s)	2470
StyI	CCWWGG	6 cut(s)	91, 1456, 1972, 2109, 2197, 2378
SwaI	ATTTAAAT	1 cut(s)	1527
TaaI	ACNGT	7 cut(s)	689, 1896, 2589, 2623, 2633, 2699, 2889
TaiI	ACGT	7 cut(s)	63, 117, 352, 361, 867, 2171, 2432
TaqI	TCGA	8 cut(s)	111, 147, 355, 682, 1222, 1575, 2191, 2612
TaqII	GACCGA	1 cut(s)	139
TatI	WGTACW	4 cut(s)	1884, 2533, 2572, 2589
TauI	GCSGC	2 cut(s)	383, 2660
TfiI	GAWTC	2 cut(s)	340, 1968
TscAI	CASTG	5 cut(s)	516, 692, 1117, 2628, 2638
TseFI	GTSAC	2 cut(s)	2501, 2646
TseI	GCWGC	5 cut(s)	827, 1601, 1868, 2255, 2654
Tsp45I	GTSAC	2 cut(s)	2501, 2646
TspGWI	ACGGA	4 cut(s)	1118, 1814, 2162, 2593
TspMI	CCCGGG	1 cut(s)	490
TspRI	CASTG	5 cut(s)	516, 692, 1117, 2628, 2638
Tth111I	GACNNNGTC	1 cut(s)	687
Vha464I	CTTAAG	2 cut(s)	410, 1996
VpaK11BI	GGWCC	4 cut(s)	122, 138, 2186, 2237
VspI	ATTAAT	1 cut(s)	1077
XapI	RAATTY	9 cut(s)	419, 1224, 1358, 2338, 2437, 2475, 2512, 2551, 2677
XceI	RCATGY	2 cut(s)	77, 2792
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2748
XmaI	CCCGGG	1 cut(s)	490
XmiI	GTMKAC	3 cut(s)	111, 575, 1938
XmnI	GAANNNNTTC	1 cut(s)	1967
XspI	CTAG	7 cut(s)	29, 1154, 1266, 1611, 1950, 2466, 2472
ZraI	GACGTC	2 cut(s)	61, 115
Zsp2I	ATGCAT	1 cut(s)	1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.