Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007016

Physical Location & Seq

Genomic Coordinates Forward (+)

31025556 .. 31035625

Gene Structure 10070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1236200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGGCGTCCCTGCGTTTTACCGCTGGCTAGCGGATCGGTACCCGCAGTCCATCGCCGACGTCGTGGAGGAGCATCCTAGAGAGGACTACAATGGCGTCCCATTGCCAGTCGACGTCTCCAGACCAAATCCTAACGGATATGAGTTCGACAACTTGTACCTTGACATGAACAACATTATACATCCATGCTTTCATCCGGACGGCAAGCCTCCCCCCGCAACCTATAATGATGTATTCAAATCAGTATTTGATTACATTGATCATCTGTTCACATTGGTTCGCCCAAGAAAGCTTCTTTTTTTGGCAATTGATGGGGTTGCTCCAAGAGCTAAAATGAATCAGCAACGTTCTCGACGTTTCCGAGCTGCAAAAGATGCGGCTGAAGCAGAAGCTGAAGAGGAAAGACTGAAGAAAGAATTTGAGATGGGAGGGGCATATTTGGTTCCTAAAGAGAAACCTGAAACTTCAGACTCAAATGTTATAACCCCAGGCACTCAATTTATGGCAGTGCTCTCGGTGGCACTTCAATATTACATACAAAGCAGGTTGAACCACAACCCTGGTTGGCAGTCTACGAAGGTTATACTTTCTGATTCGAATGTTCCTGGCGAGGGAGAGCACAAAATAATGTCATACATCCGGTTGCAACGCAACCTCCCTGGTTTTGACCCTAACACTCGACATTGTCTTTATGGTTTGGATGCAGATCTGATTATGCTGGCTTTAGCTACACACGAGGTTCACTTCTCAATATTAAGAGAGGTGATTACTTTTCCAGGTCAACAAGAAAAATGTTTTCTTTGTGGACAAGTTGGTCATCTGGCAGCTGAGTGTCGTGGTGTACCTGATGATAATGGAAATGTGGTTGATGAAACACCAATTCACCAGAAAAAATATCAGTTCCTCAATGTATGGGTATTGCGTGAATATTTGCAATATGAGCTGGATATTCCCAACCCTCCTTTTCCGCTCAATTTTGAAAGGATAGTGGATGATTTTGTTTTTATGTGTTTTTTTGTTGGCAATGACTTTCTTCCACATATGCCAACATTGGAGATCAGAGAGGGTGCTATTAATTTATTGATGCATATTTATAGAAAGGAGTTTACTGCTATGGGAGGTTATCTTACTGATGCGGGTGAGGTATTGTTAGACAGAGTGGAGCATTTTATTCAGTCTGTTGCTGTTTTTGAACAACAAATTTTTCAAAAGCGGGTTCGAATTCAGCAGGCTATTGAGAATAATGAAGAGAGACATAGAGCTAGAAGAGAAACTTCAGCAGAGCTACCGGTTCCGGTGATAGACAAGGTTAAATTGGGAGAGCCTGGATACACAGAAAGGTATTATGCTGAGAAATTTCAAGTATCAAAACCAGAGGAGATTGATAAAGTCAAAAAGGACTTGGTTTTAAAATATGTAGAAGGCTTATGTTGGGTTTGCCGATATTATTACCAAGGAGTCTGCTCATGGCAATGGTATTATCCATACCATTATGCTCCTTTTGCTTCTGATCTGAAAGATTTAGATGAATTGGAAATAACATTTTTCTTGGGAGAGCCATTTAAGCCCTTCGATCAGCTCATGGGAACCTTACCAGCCGCAAGCTCCAGTGCTCTTCCGGAAAAATACAGAAACTTGATGAGTGATCCAAGATCTCCTATCCATGATTTCTACCCTGCTGATTTTGAAATTGACATGAATGGCAAGCGCTTTGCATGGCAGGGCGTTGCCAAATTGCCATTCATTGATGAGAAGAAATTGCTTACGGAGACAAGGAAGCTTGAAAGCACTTTAACGGAGGAAGAGCAGGCTCGGAATAGCATAATGCTTGATTTGCTTTATGTTTGTACTTCTCATCCTTTGGCAGCACACATCATTCCGTACTACCAACTGTGTTATCAGACACCCCCACATGAAAGATGTTTATGGATCATAGACACAATTGCTAGTGGTGGAATGAATGGATTCCTTTGGTTATCTGAGAGAAATGGCGTTAGACATGTAATCCCTTCCCCTGTTCATGGTTATCCAAACCTTCATTATAACCAAGTCTTAAATGTTACCTATTTTTACCCCACAGCTCATACACACATTCCAGAACCTCCCGAGGGTGTGATAATGCCCAGAAAGGTTGTAAGACCTACGGACATCAAACCATTCCCTACGTTATGGCACGACGAAGGTCCTCGACGCCAAGGTAGAGAGAGGCCACAAATACCTGGAGCAATTGCTGGATCCATGTTGGGAGAAGCAGCTCATCGACTAGTCAAGAACACCCTTAATATTAGACCTAGTAGTTCATCCTCCGGATTTTGGGATCAACCGACATTACGAAATTCTGGAAACTATGCAGGAAATTATCCAGTCAACCGACCAAGATCATCTGGGCCCTCTGGGTATGAGAGTGGCTTCCGCGAAGAAGCAAAGTACGGAAATTCCTTCAATCCTCAGGTCATGATGATTAGGCCTAGATTTCTCGCATCAAATGGTATGCAAGGTGGCAGACAGAATTTTAGGGCACAAGAAAGAGTACAATATCAAGAACAGAGTTTTAGGACACAAGAGGGAGTACAATATCAAGAACAGTACCACAACCTGAGAACCGCGATGTCTAGTTTAACAGTTCAAGGCAGTGTGAGAACCAGGTCACCTGCAGCGGCGCCACCTGGGATGCCAAATTCAGGATACCAAGCAAACCATCAGCACCAGTTTGTGCAGAACATGGGTGCCCTTCCTTCGCCACCTACCAAGTGGATTAGCAAAGAACCAACTGGAAATGGTGGATTATTTGTAAGGCAACAGGAAACTGGATATGGTGGAGCATATGAGCAACCGCAGGTCAAGGTCTATCAAGTTAAGACGCGTGCTCCCCAGGATACGTCAGACCGTGGAGACCAGAGCGGGAGTGATTCCTCAGGATTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000291 GO:0000902 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009826 GO:0009888 GO:0009892 GO:0009987 GO:0010087 GO:0010286 GO:0010468 GO:0010494 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016049 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0031086 GO:0031087 GO:0032502 GO:0032989 GO:0032991 GO:0033554 GO:0034605 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0040007 GO:0040029 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051301 GO:0051716 GO:0060255 GO:0060560 GO:0065007 GO:0070370 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 ko04016 map03008 map03018 map04016

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

976

Amino Acids

111.47

Weight (kDa)

6.23

Isoelectric Point (pI)

50.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 254	9.2e-99	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	324 - 426	1e-42	Xrn1 helical domain
XRN_M	PF17846	434 - 717	5.1e-114	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54490 AT1G54490
fragaria_vesca	FvH4_6g03690 FvH4_6g03690
malus_domestica	MD04G1219200.v1.1 MD12G1236200.v1.1
prunus_persica	Prupe.6G338500_v2.0.a1 Prupe.6G338500_v2.0.a1
pyrus_communis	pycom04g19450 pycom12g21730
rosa_chinensis	RchiOBHm_Chr3g0452231
rosa_laevigata	RLG00000025615
rosa_multiflora	Rmu_sc0037132.1_g000001 Rmu_sc0041263.1_g000001
rosa_roxburghii	Rroxscaffold_164G00435930 Rroxscaffold_6G00429350
rosa_rugosa	Rorug02G0639400
rosa_samantha	Rh3AG041300 Rh3BG043000 Rh3BG043200 Rh3CG041400 Rh3CG041600 Rh3DG042300 Rh3DG042500
rosa_wichuraiana	Rw3G003160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	482, 2043
AarI	CACCTGC	1 cut(s)	2659
AasI	GACNNNNNNGTC	1 cut(s)	813
AatII	GACGTC	2 cut(s)	63, 117
Acc36I	ACCTGC	3 cut(s)	534, 2659, 2827
Acc65I	GGTACC	1 cut(s)	39
AccB1I	GGYRCC	3 cut(s)	39, 2659, 2726
AccBSI	CCGCTC	2 cut(s)	970, 2901
AccI	GTMKAC	2 cut(s)	111, 572
AccII	CGCG	3 cut(s)	2415, 2606, 2863
AccIII	TCCGGA	3 cut(s)	196, 1618, 2306
AclI	AACGTT	1 cut(s)	346
AclWI	GGATC	6 cut(s)	42, 1640, 1937, 2229, 2242, 2325
AcsI	RAATTY	8 cut(s)	416, 1200, 1221, 1355, 2335, 2434, 2509, 2677
AcuI	CTGAAG	5 cut(s)	402, 414, 428, 450, 1260
AcyI	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
AfaI	GTAC	9 cut(s)	41, 158, 843, 1849, 1883, 2429, 2532, 2571, 2588
AfeI	AGCGCT	1 cut(s)	1709
AfiI	CCNNNNNNNGG	5 cut(s)	611, 2021, 2108, 2313, 2681
AflIII	ACRYGT	2 cut(s)	1999, 2861
AgeI	ACCGGT	1 cut(s)	1288
AhlI	ACTAGT	1 cut(s)	2263
AjuI	GAANNNNNNNTTGG	2 cut(s)	1724, 1756
Alw21I	GWGCWC	4 cut(s)	513, 621, 1615, 2869
Alw26I	GTCTC	4 cut(s)	121, 1246, 1763, 2886
AlwI	GGATC	6 cut(s)	42, 1640, 1937, 2229, 2242, 2325
AlwNI	CAGNNNCTG	1 cut(s)	392
Ama87I	CYCGRG	1 cut(s)	2105
Aor13HI	TCCGGA	3 cut(s)	196, 1618, 2306
Aor51HI	AGCGCT	1 cut(s)	1709
AoxI	GGCC	3 cut(s)	2207, 2387, 2465
ApaI	GGGCCC	1 cut(s)	2391
ApeKI	GCWGC	5 cut(s)	365, 824, 1865, 2252, 2654
ApoI	RAATTY	8 cut(s)	416, 1200, 1221, 1355, 2335, 2434, 2509, 2677
AseI	ATTAAT	1 cut(s)	1074
AsiGI	ACCGGT	1 cut(s)	1288
Asp700I	GAANNNNTTC	1 cut(s)	1964
Asp718I	GGTACC	1 cut(s)	39
AspLEI	GCGC	2 cut(s)	1710, 2662
AspS9I	GGNCC	3 cut(s)	2183, 2387, 2388
AsuHPI	GGTGA	5 cut(s)	775, 875, 1151, 1309, 2640
AsuII	TTCGAA	2 cut(s)	596, 1219
AsuNHI	GCTAGC	1 cut(s)	28
AvaI	CYCGRG	1 cut(s)	2105
AvaII	GGWCC	1 cut(s)	2183
AxyI	CCTNAGG	2 cut(s)	2448, 2914
BaeGI	GKGCMC	3 cut(s)	2391, 2521, 2731
BamHI	GGATCC	1 cut(s)	2234
BanI	GGYRCC	3 cut(s)	39, 2659, 2726
BanII	GRGCYC	1 cut(s)	2391
BauI	CACGAG	1 cut(s)	734
Bbv12I	GWGCWC	4 cut(s)	513, 621, 1615, 2869
BbvI	GCAGC	5 cut(s)	352, 836, 1877, 2264, 2666
BccI	CCATC	4 cut(s)	59, 305, 418, 2706
BceAI	ACGGC	1 cut(s)	217
BcgI	CGANNNNNNTGC	4 cut(s)	332, 366, 495, 529
BciVI	GTATCC	3 cut(s)	1322, 2678, 2869
BclI	TGATCA	1 cut(s)	259
BcoDI	GTCTC	4 cut(s)	121, 1246, 1763, 2886
BcuI	ACTAGT	1 cut(s)	2263
BfaI	CTAG	8 cut(s)	29, 78, 1263, 1947, 2264, 2292, 2469, 2613
BfmI	CTRYAG	1 cut(s)	2652
BfoI	RGCGCY	2 cut(s)	1711, 2663
BfuAI	ACCTGC	3 cut(s)	534, 2659, 2827
BfuI	GTATCC	3 cut(s)	1322, 2678, 2869
BglII	AGATCT	2 cut(s)	706, 1652
BisI	GCNGC	8 cut(s)	366, 378, 825, 1599, 1866, 2253, 2655, 2658
BlsI	GCNGC	8 cut(s)	367, 379, 826, 1600, 1867, 2254, 2656, 2659
Bme18I	GGWCC	1 cut(s)	2183
BmeT110I	CYCGRG	1 cut(s)	2105
BmgT120I	GGNCC	3 cut(s)	2183, 2387, 2388
BmiI	GGNNCC	8 cut(s)	41, 444, 1293, 1588, 2236, 2389, 2661, 2728
BmsI	GCATC	7 cut(s)	82, 364, 691, 1074, 1123, 2489, 2661
BmtI	GCTAGC	1 cut(s)	32
BoxI	GACNNNNGTC	1 cut(s)	2181
BpmI	CTGGAG	3 cut(s)	103, 1591, 2241
Bpu14I	TTCGAA	2 cut(s)	596, 1219
BsaBI	GATNNNNATC	1 cut(s)	705
BsaHI	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
BsaI	GGTCTC	1 cut(s)	2886
BsaJI	CCNNGG	9 cut(s)	487, 559, 658, 1453, 2106, 2194, 2666, 2871, 2887
BsaWI	WCCGGW	6 cut(s)	196, 639, 1288, 1294, 1618, 2306
BsaXI	ACNNNNNCTCC	4 cut(s)	639, 669, 2851, 2881
Bsc4I	CCNNNNNNNGG	5 cut(s)	611, 2021, 2108, 2313, 2681
Bse118I	RCCGGY	1 cut(s)	1288
Bse1I	ACTGG	6 cut(s)	107, 1608, 2363, 2707, 2776, 2812
Bse21I	CCTNAGG	2 cut(s)	2448, 2914
Bse3DI	GCAATG	3 cut(s)	101, 1030, 1478
Bse8I	GATNNNNATC	1 cut(s)	705
BseAI	TCCGGA	3 cut(s)	196, 1618, 2306
BseDI	CCNNGG	9 cut(s)	487, 559, 658, 1453, 2106, 2194, 2666, 2871, 2887
BseGI	GGATG	9 cut(s)	73, 181, 193, 636, 706, 997, 1855, 2300, 2676
BseJI	GATNNNNATC	1 cut(s)	705
BseLI	CCNNNNNNNGG	5 cut(s)	611, 2021, 2108, 2313, 2681
BseMI	GCAATG	3 cut(s)	101, 1030, 1478
BseMII	CTCAG	6 cut(s)	819, 1341, 1971, 2462, 2588, 2928
BseNI	ACTGG	6 cut(s)	107, 1608, 2363, 2707, 2776, 2812
BseRI	GAGGAG	2 cut(s)	83, 1391
BseSI	GKGCMC	3 cut(s)	2391, 2521, 2731
BseXI	GCAGC	5 cut(s)	352, 836, 1877, 2264, 2666
BsgI	GTGCAG	1 cut(s)	2735
Bsh1236I	CGCG	3 cut(s)	2415, 2606, 2863
BshFI	GGCC	3 cut(s)	2209, 2389, 2467
BshNI	GGYRCC	3 cut(s)	39, 2659, 2726
BshTI	ACCGGT	1 cut(s)	1288
BsiHKAI	GWGCWC	4 cut(s)	513, 621, 1615, 2869
BsiHKCI	CYCGRG	1 cut(s)	2105
BsiSI	CCGG	6 cut(s)	197, 640, 1289, 1295, 1619, 2307
BslFI	GGGAC	1 cut(s)	83
BslI	CCNNNNNNNGG	5 cut(s)	611, 2021, 2108, 2313, 2681
BsmAI	GTCTC	4 cut(s)	121, 1246, 1763, 2886
BsmBI	CGTCTC	1 cut(s)	121
BsmFI	GGGAC	1 cut(s)	83
BsnI	GGCC	3 cut(s)	2209, 2389, 2467
Bso31I	GGTCTC	1 cut(s)	2886
BsoBI	CYCGRG	1 cut(s)	2105
Bsp119I	TTCGAA	2 cut(s)	596, 1219
Bsp120I	GGGCCC	1 cut(s)	2387
Bsp1286I	GDGCHC	7 cut(s)	513, 621, 1615, 2391, 2521, 2731, 2869
Bsp13I	TCCGGA	3 cut(s)	196, 1618, 2306
BspANI	GGCC	3 cut(s)	2209, 2389, 2467
BspCNI	CTCAG	6 cut(s)	820, 1342, 1972, 2461, 2589, 2927
BspEI	TCCGGA	3 cut(s)	196, 1618, 2306
BspFNI	CGCG	3 cut(s)	2415, 2606, 2863
BspHI	TCATGA	1 cut(s)	2454
BspLI	GGNNCC	8 cut(s)	41, 444, 1293, 1588, 2236, 2389, 2661, 2728
BspMAI	CTGCAG	1 cut(s)	2656
BspMI	ACCTGC	3 cut(s)	534, 2659, 2827
BspOI	GCTAGC	1 cut(s)	32
BspPI	GGATC	6 cut(s)	42, 1640, 1937, 2229, 2242, 2325
BspQI	GCTCTTC	2 cut(s)	1620, 1797
BspT104I	TTCGAA	2 cut(s)	596, 1219
BspT107I	GGYRCC	3 cut(s)	39, 2659, 2726
BspTNI	GGTCTC	1 cut(s)	2886
BsrBI	CCGCTC	2 cut(s)	970, 2901
BsrDI	GCAATG	3 cut(s)	101, 1030, 1478
BsrFI	RCCGGY	1 cut(s)	1288
BsrI	ACTGG	6 cut(s)	107, 1608, 2363, 2707, 2776, 2812
BssAI	RCCGGY	1 cut(s)	1288
BssECI	CCNNGG	9 cut(s)	487, 559, 658, 1453, 2106, 2194, 2666, 2871, 2887
BssNI	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
BssSI	CACGAG	1 cut(s)	734
BssT1I	CCWWGG	2 cut(s)	1453, 2194
Bst2BI	CACGAG	1 cut(s)	734
Bst4CI	ACNGT	4 cut(s)	1893, 2586, 2623, 2888
Bst6I	CTCTTC	5 cut(s)	390, 1242, 1261, 1620, 1797
BstACI	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
BstAPI	GCANNNNNTGC	1 cut(s)	374
BstBI	TTCGAA	2 cut(s)	596, 1219
BstC8I	GCNNGC	9 cut(s)	26, 30, 206, 720, 1230, 1603, 1706, 1809, 2865
BstDEI	CTNAG	6 cut(s)	828, 1350, 1980, 2448, 2597, 2914
BstDSI	CCRYGG	1 cut(s)	2887
BstEII	GGTNACC	1 cut(s)	2646
BstF5I	GGATG	9 cut(s)	73, 181, 193, 636, 706, 997, 1855, 2300, 2676
BstFNI	CGCG	3 cut(s)	2415, 2606, 2863
BstH2I	RGCGCY	2 cut(s)	1711, 2663
BstHHI	GCGC	2 cut(s)	1710, 2662
BstMAI	GTCTC	4 cut(s)	121, 1246, 1763, 2886
BstMWI	GCNNNNNNNGC	7 cut(s)	326, 374, 383, 940, 1502, 1564, 1834
BstNSI	RCATGY	1 cut(s)	2003
BstPAI	GACNNNNGTC	1 cut(s)	2181
BstPI	GGTNACC	1 cut(s)	2646
BstSFI	CTRYAG	1 cut(s)	2652
BstSLI	GKGCMC	3 cut(s)	2391, 2521, 2731
BstUI	CGCG	3 cut(s)	2415, 2606, 2863
BstV1I	GCAGC	5 cut(s)	352, 836, 1877, 2264, 2666
BstX2I	RGATCY	3 cut(s)	706, 1652, 2234
BstXI	CCANNNNNNTGG	1 cut(s)	560
BstYI	RGATCY	3 cut(s)	706, 1652, 2234
Bsu36I	CCTNAGG	2 cut(s)	2448, 2914
BsuI	GTATCC	3 cut(s)	1322, 2678, 2869
BsuRI	GGCC	3 cut(s)	2209, 2389, 2467
BtgI	CCRYGG	1 cut(s)	2887
BtgZI	GCGATG	2 cut(s)	37, 2621
BtsCI	GGATG	9 cut(s)	73, 181, 193, 636, 706, 997, 1855, 2300, 2676
BtsI	GCAGTG	2 cut(s)	513, 2638
BtsIMutI	CAGTG	3 cut(s)	513, 1615, 2638
BveI	ACCTGC	3 cut(s)	534, 2659, 2827
Cac8I	GCNNGC	9 cut(s)	26, 30, 206, 720, 1230, 1603, 1706, 1809, 2865
CaiI	CAGNNNCTG	1 cut(s)	392
CciI	TCATGA	1 cut(s)	2454
CfoI	GCGC	2 cut(s)	1710, 2662
Cfr10I	RCCGGY	1 cut(s)	1288
Cfr13I	GGNCC	3 cut(s)	2183, 2387, 2388
CseI	GACGC	3 cut(s)	85, 2199, 2869
CsiI	ACCWGGT	1 cut(s)	2642
Csp6I	GTAC	9 cut(s)	40, 157, 842, 1848, 1882, 2428, 2531, 2570, 2587
CspAI	ACCGGT	1 cut(s)	1288
CviQI	GTAC	9 cut(s)	40, 157, 842, 1848, 1882, 2428, 2531, 2570, 2587
DdeI	CTNAG	6 cut(s)	828, 1350, 1980, 2448, 2597, 2914
DinI	GGCGCC	1 cut(s)	2661
DraI	TTTAAA	1 cut(s)	1410
DrdI	GACNNNNNNGTC	1 cut(s)	813
DseDI	GACNNNNNNGTC	1 cut(s)	813
Eam1104I	CTCTTC	5 cut(s)	390, 1242, 1261, 1620, 1797
EarI	CTCTTC	5 cut(s)	390, 1242, 1261, 1620, 1797
Eco130I	CCWWGG	2 cut(s)	1453, 2194
Eco147I	AGGCCT	1 cut(s)	2467
Eco24I	GRGCYC	1 cut(s)	2391
Eco31I	GGTCTC	1 cut(s)	2886
Eco47I	GGWCC	1 cut(s)	2183
Eco47III	AGCGCT	1 cut(s)	1709
Eco57I	CTGAAG	5 cut(s)	402, 414, 428, 450, 1260
Eco81I	CCTNAGG	2 cut(s)	2448, 2914
Eco88I	CYCGRG	1 cut(s)	2105
Eco91I	GGTNACC	1 cut(s)	2646
EcoO109I	RGGNCCY	2 cut(s)	2183, 2388
EcoO65I	GGTNACC	1 cut(s)	2646
EcoRI	GAATTC	1 cut(s)	1221
EcoT14I	CCWWGG	2 cut(s)	1453, 2194
EcoT22I	ATGCAT	1 cut(s)	1089
EcoT38I	GRGCYC	1 cut(s)	2391
EgeI	GGCGCC	1 cut(s)	2661
EheI	GGCGCC	1 cut(s)	2661
ErhI	CCWWGG	2 cut(s)	1453, 2194
Esp3I	CGTCTC	1 cut(s)	121
FalI	AAGNNNNNCTT	2 cut(s)	1258, 1290
FaqI	GGGAC	1 cut(s)	83
FauI	CCCGC	5 cut(s)	51, 223, 1129, 1206, 2894
FauNDI	CATATG	2 cut(s)	1041, 2824
FbaI	TGATCA	1 cut(s)	259
FblI	GTMKAC	2 cut(s)	111, 572
Fnu4HI	GCNGC	8 cut(s)	366, 378, 825, 1599, 1866, 2253, 2655, 2658
FokI	GGATG	9 cut(s)	60, 168, 180, 623, 713, 1004, 1842, 2287, 2683
FriOI	GRGCYC	1 cut(s)	2391
Fsp4HI	GCNGC	8 cut(s)	366, 378, 825, 1599, 1866, 2253, 2655, 2658
FspBI	CTAG	8 cut(s)	29, 78, 1263, 1947, 2264, 2292, 2469, 2613
GlaI	GCGC	2 cut(s)	1709, 2661
GluI	GCNGC	8 cut(s)	366, 378, 825, 1599, 1866, 2253, 2655, 2658
GsuI	CTGGAG	3 cut(s)	103, 1591, 2241
HaeII	RGCGCY	2 cut(s)	1711, 2663
HaeIII	GGCC	3 cut(s)	2209, 2389, 2467
HapII	CCGG	6 cut(s)	197, 640, 1289, 1295, 1619, 2307
HgaI	GACGC	3 cut(s)	85, 2199, 2869
HhaI	GCGC	2 cut(s)	1710, 2662
Hin1I	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
Hin6I	GCGC	2 cut(s)	1708, 2660
HinP1I	GCGC	2 cut(s)	1708, 2660
HincII	GTYRAC	3 cut(s)	112, 782, 2368
HindII	GTYRAC	3 cut(s)	112, 782, 2368
HindIII	AAGCTT	2 cut(s)	290, 1778
HinfI	GANTC	6 cut(s)	337, 470, 593, 1458, 1965, 2909
HpaII	CCGG	6 cut(s)	197, 640, 1289, 1295, 1619, 2307
HphI	GGTGA	5 cut(s)	775, 875, 1151, 1309, 2640
Hpy166II	GTNNAC	9 cut(s)	112, 270, 573, 742, 782, 806, 842, 1107, 2368
Hpy8I	GTNNAC	9 cut(s)	112, 270, 573, 742, 782, 806, 842, 1107, 2368
Hpy99I	CGWCG	6 cut(s)	62, 65, 116, 357, 2180, 2193
HpyAV	CCTTC	9 cut(s)	571, 1415, 1579, 2019, 2045, 2174, 2449, 2741, 2745
HpyCH4III	ACNGT	4 cut(s)	1893, 2586, 2623, 2888
HpyCH4IV	ACGT	6 cut(s)	60, 114, 346, 355, 2165, 2879
HpyF10VI	GCNNNNNNNGC	7 cut(s)	326, 374, 383, 940, 1502, 1564, 1834
HpyF3I	CTNAG	6 cut(s)	828, 1350, 1980, 2448, 2597, 2914
HpySE526I	ACGT	6 cut(s)	60, 114, 346, 355, 2165, 2879
Hsp92I	GRCGYC	6 cut(s)	6, 60, 96, 114, 2191, 2660
HspAI	GCGC	2 cut(s)	1708, 2660
KasI	GGCGCC	1 cut(s)	2659
Kpn2I	TCCGGA	3 cut(s)	196, 1618, 2306
KpnI	GGTACC	1 cut(s)	43
Ksp22I	TGATCA	1 cut(s)	259
LguI	GCTCTTC	2 cut(s)	1620, 1797
LmnI	GCTCC	8 cut(s)	70, 325, 1162, 1501, 1610, 2222, 2819, 2872
Lsp1109I	GCAGC	5 cut(s)	352, 836, 1877, 2264, 2666
LweI	GCATC	7 cut(s)	82, 364, 691, 1074, 1123, 2489, 2661
MabI	ACCWGGT	1 cut(s)	2642
MaeI	CTAG	8 cut(s)	29, 78, 1263, 1947, 2264, 2292, 2469, 2613
MaeII	ACGT	6 cut(s)	60, 114, 346, 355, 2165, 2879
MaeIII	GTNAC	2 cut(s)	2059, 2646
MbiI	CCGCTC	2 cut(s)	970, 2901
MboII	GAAGA	9 cut(s)	407, 421, 1025, 1259, 1278, 1607, 1765, 1814, 2429
MfeI	CAATTG	3 cut(s)	306, 1941, 2226
MflI	RGATCY	3 cut(s)	706, 1652, 2234
MhlI	GDGCHC	7 cut(s)	513, 621, 1615, 2391, 2521, 2731, 2869
MluI	ACGCGT	1 cut(s)	2861
Mly113I	GGCGCC	1 cut(s)	2660
MlyI	GAGTC	2 cut(s)	464, 1467
Mph1103I	ATGCAT	1 cut(s)	1089
MroI	TCCGGA	3 cut(s)	196, 1618, 2306
MroXI	GAANNNNTTC	1 cut(s)	1964
MslI	CAYNNNNRTG	1 cut(s)	1471
MspA1I	CMGCKG	3 cut(s)	24, 827, 2657
MspI	CCGG	6 cut(s)	197, 640, 1289, 1295, 1619, 2307
MunI	CAATTG	3 cut(s)	306, 1941, 2226
MvnI	CGCG	3 cut(s)	2415, 2606, 2863
MwoI	GCNNNNNNNGC	7 cut(s)	326, 374, 383, 940, 1502, 1564, 1834
NarI	GGCGCC	1 cut(s)	2660
NdeI	CATATG	2 cut(s)	1041, 2824
NheI	GCTAGC	1 cut(s)	28
NlaIV	GGNNCC	8 cut(s)	41, 444, 1293, 1588, 2236, 2389, 2661, 2728
NmuCI	GTSAC	1 cut(s)	2646
NsiI	ATGCAT	1 cut(s)	1089
NspI	RCATGY	1 cut(s)	2003
NspV	TTCGAA	2 cut(s)	596, 1219
PagI	TCATGA	1 cut(s)	2454
PaqCI	CACCTGC	1 cut(s)	2659
PceI	AGGCCT	1 cut(s)	2467
PciI	ACATGT	1 cut(s)	1999
PciSI	GCTCTTC	2 cut(s)	1620, 1797
PcsI	WCGNNNNNNNCGW	3 cut(s)	60, 352, 358
PdmI	GAANNNNTTC	1 cut(s)	1964
PfeI	GAWTC	4 cut(s)	337, 593, 1965, 2909
PflFI	GACNNNGTC	1 cut(s)	684
PinAI	ACCGGT	1 cut(s)	1288
PkrI	GCNGC	8 cut(s)	367, 379, 826, 1600, 1867, 2254, 2656, 2659
PleI	GAGTC	2 cut(s)	464, 1466
PluTI	GGCGCC	1 cut(s)	2663
PpsI	GAGTC	2 cut(s)	464, 1466
PpuMI	RGGWCCY	1 cut(s)	2183
PscI	ACATGT	1 cut(s)	1999
PshAI	GACNNNNGTC	1 cut(s)	2181
PshBI	ATTAAT	1 cut(s)	1074
PsiI	TTATAA	2 cut(s)	482, 2043
Psp1406I	AACGTT	1 cut(s)	346
Psp5II	RGGWCCY	1 cut(s)	2183
PspEI	GGTNACC	1 cut(s)	2646
PspN4I	GGNNCC	8 cut(s)	41, 444, 1293, 1588, 2236, 2389, 2661, 2728
PspOMI	GGGCCC	1 cut(s)	2387
PspPI	GGNCC	3 cut(s)	2183, 2387, 2388
PspPPI	RGGWCCY	1 cut(s)	2183
PstI	CTGCAG	1 cut(s)	2656
PstNI	CAGNNNCTG	1 cut(s)	392
PsuI	RGATCY	3 cut(s)	706, 1652, 2234
PsyI	GACNNNGTC	1 cut(s)	684
PvuII	CAGCTG	1 cut(s)	827
RsaI	GTAC	9 cut(s)	41, 158, 843, 1849, 1883, 2429, 2532, 2571, 2588
RsaNI	GTAC	9 cut(s)	40, 157, 842, 1848, 1882, 2428, 2531, 2570, 2587
RseI	CAYNNNNRTG	1 cut(s)	1471
SalI	GTCGAC	1 cut(s)	110
SapI	GCTCTTC	2 cut(s)	1620, 1797
SatI	GCNGC	8 cut(s)	366, 378, 825, 1599, 1866, 2253, 2655, 2658
Sau96I	GGNCC	3 cut(s)	2183, 2387, 2388
SchI	GAGTC	2 cut(s)	464, 1467
SduI	GDGCHC	7 cut(s)	513, 621, 1615, 2391, 2521, 2731, 2869
SexAI	ACCWGGT	1 cut(s)	2642
SfaNI	GCATC	7 cut(s)	82, 364, 691, 1074, 1123, 2489, 2661
SfcI	CTRYAG	1 cut(s)	2652
SfoI	GGCGCC	1 cut(s)	2661
SfuI	TTCGAA	2 cut(s)	596, 1219
SinI	GGWCC	1 cut(s)	2183
SmiMI	CAYNNNNRTG	1 cut(s)	1471
SpeI	ACTAGT	1 cut(s)	2263
SseBI	AGGCCT	1 cut(s)	2467
SspDI	GGCGCC	1 cut(s)	2659
SspI	AATATT	4 cut(s)	530, 753, 929, 2284
SspMI	CTAG	8 cut(s)	29, 78, 1263, 1947, 2264, 2292, 2469, 2613
StuI	AGGCCT	1 cut(s)	2467
StyI	CCWWGG	2 cut(s)	1453, 2194
TaaI	ACNGT	4 cut(s)	1893, 2586, 2623, 2888
TaiI	ACGT	6 cut(s)	63, 117, 349, 358, 2168, 2882
TaqI	TCGA	9 cut(s)	111, 147, 352, 596, 679, 1219, 1572, 2188, 2260
TatI	WGTACW	3 cut(s)	1847, 2530, 2569
TauI	GCSGC	3 cut(s)	380, 1601, 2660
TfiI	GAWTC	4 cut(s)	337, 593, 1965, 2909
TscAI	CASTG	3 cut(s)	513, 1615, 2638
TseFI	GTSAC	1 cut(s)	2646
TseI	GCWGC	5 cut(s)	365, 824, 1865, 2252, 2654
Tsp45I	GTSAC	1 cut(s)	2646
TspGWI	ACGGA	6 cut(s)	150, 1781, 1811, 1869, 2159, 2445
TspRI	CASTG	3 cut(s)	513, 1615, 2638
Tth111I	GACNNNGTC	1 cut(s)	684
VpaK11BI	GGWCC	1 cut(s)	2183
VspI	ATTAAT	1 cut(s)	1074
XapI	RAATTY	8 cut(s)	416, 1200, 1221, 1355, 2335, 2434, 2509, 2677
XceI	RCATGY	1 cut(s)	2003
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2748
XmiI	GTMKAC	2 cut(s)	111, 572
XmnI	GAANNNNTTC	1 cut(s)	1964
XspI	CTAG	8 cut(s)	29, 78, 1263, 1947, 2264, 2292, 2469, 2613
ZraI	GACGTC	2 cut(s)	61, 115
Zsp2I	ATGCAT	1 cut(s)	1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.