Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003901

Physical Location & Seq

Genomic Coordinates Forward (+)

30973496 .. 30978909

Gene Structure 5414 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1229500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGAGGATTATGATCAAAGGAGGTGTGTGGAAGAACACGGAGGATGAGATCCTCAAGGCGGCGGTCATGAAGTATGGGAAGAACCAGTGGGCTCGAATCTCGTCGCTCTTGGTTCGCAAATCGGCTAAACAGTGTAAGGCGAGATGGTACGAGTGGCTCGACCCCTCCATCAAAAAGATGACTGAGTGGACTAGAGAAGAGGATGAGAAGCTTCTACATCTTGCTAAGCTCATGCCTACACAATGGAGAACCATTGCCCCGATTGTTGGTCGTACTCCGTCGCAGTGCCTCGAGCGGTATGAGAAACTGCTTGATGCAGCTTGTGTTAAGGATGAGAATTATGAACCGGGTGATGATCCACGGAAGTTGCGGCCTGGAGAGATTGACCCAAATCCCGAGTCGAAACCTGCTCGTCCTGATCCTGTTGATATGGATGAGGATGAGAAGGAGATGCTTTCAGAGGCGCGAGCTCGGTTAGCAAACACTAGGGGAAAGAAGGCTAAAAGGAAAGCCAGGGAGAAACAGCTTGAAGAGGCCAGAAGGCTTGCTTCGCTGCAGAAAAGGAGAGAATTAAAGGCTGCTGGCATTGATACTAGGCAGCGAAAGAGGAAAAGGAAGGGAATAGACTACAATGCGGAAATTCCCTTTGAGAAAAAGCCTCCTCCAGGCTTTTATGATGTTGCTGATGAAGATAGACCAGTGGAGCAGCCTAACTTCCCTACGACTATTGAGGAACTCGAGGGGAAGAGAAGGATTGATGTGGAAGCGCAGTTGAGAAAGCAAGATATTGCTAAGAACAAAATTGCTCAAAGACAGGATGCTCCATCAGCTATACTACAAGCCAACAAGATGAATGATCCGGAAACGGTTAGGAAGAGATCAAAACTGATGCTTCCTGCACCCCAAATTTCGGACAATGAGTTGGAGGAAATTGCAAAGATGGGTTATGCTAATGATCTTGCAGCTGAAGAATTTGCTGAAGGTAGTGGTGCAACTCGAGCTCTTCTTGCAAATTATTCCCAGACACCAAGGCTGGGAATGACACCTTCACGAACACCTCAAAGAACACCTACTGGTAAGGGTGATGCTATAATGATGGAAGCAGAAAATTTGGCCAGGTTGAGAGAGTCTCAGACACCATTATTAGGAGGAGATAATCCAGAGTTGCACCCATCAGATTTTTCTGGGGTCACTCCTAGAAAAAAGGAAATTCAAACTCCAAATATGATGCTTACTCCTTCTGCAACTCCTGGAGGTGCCGGCCTGACTCCAAGAACGGGTCTTACGCCTAGAATTGGCATGACGCCATCAAGGGATTCTTTTGGCATGACCCCAAAGGGGACTCCCATTAGGGATGAGCTTCGTATCAATGAAGAAATAGATACACCTAATAGTGCAAAACTGGAGCAGCGAAGAAACTTGCAGTTTGGTTTTGGAAGTTTACCACAGCCTAAGAATGAGTACCAAATTGTCGTACAACCAGCTCCAGAGGATAATGAAGAACCTGAGGAGAAGATTGAAGAGGACATGTCTGATAGGCTAGCTAGAGAAAGGGCTGAGGAAGAAGCACGCCAGCAAGCATTACTTAGGAAGAGATCAAAAGTATTGCAGAGGGAGCTCCCTAGACCTCCTGCTGCCTCATTGGAACTCATCAGAAATTCCTTGATAAAAGCAGATGGAGATAGGAGTTCATTTGTTCCTCCTACTCATATTGAGCAGGCTGATGAAATGGTTAAAGCAGAACTTCTTAGCCTACTAGAGCATGATAATGCCAAGTATCCGCTTACTGAGAAAGTAGACAAACGAAAAAAGAAAGGTTCCAAGCGCAGTGCCAATGGACCTAGTACTGTCCCTGTGATAGAAGATTTTGAAGAAGATGAACTGAAAGATGCTGATTCATTGATAAAGGAAGATGCAGAAATTCTTCGTGTTGCAATGGGGCATGAAAACGAATCCCTTGATGAATTTCTTGAAGCACACAAAACTTGCCTGAACGATCTCATGTACTTCCCTACTCGTGGAGCTTATGGTCTATCAAGTATGGATGATGATATCCAGAAGGCAGCAAGCATTGAGAACAAGGTTAAAGTCCGCACATATGGTTATGAGATGCGGGCTAAAGAGGGTCTCTGGCCAAAAATTGAGGAAACGTTTAAGCAGATTGACACCGCTGCAAAAGAACTTGAGTGCTTTAAAGCTTTACAGAAGCAAGAAAGATTAGCGGCAACACACAGGATAAATAATATCTGGGAGGAAGTTCAGAATCAAAAGGAGCTTGAGCGGCAACTACAGAAGCAGTATGGGGATCTTGTAGTCGAGGTGGAAAGGGTACAGCACCGCATGGATGAACTCAGAGCAGAAGCAGAAAAACAAGAAATTGCTGCGAGGAATTCTGATCTGGAGTTGCCTGAGGCTGCAGCAGATGTGACTGTTCTGCAGACAACTGAAAATCCTGATCCTGTAACTGCCTCAGATGAGCTTGGAAAAGCTGTTCCCGGTGGTGCATCTGATGGTGAAGCAACAATTCAGCAAATGGATGGTGTGCAGGAACAGGCTTCAACAGCCCCAGAAGGTGATATGGACATTGATGCCGAAAAGGTTCATGCGATGCCAGCGGAGGATGTAAACTTACCTGATAAGATGCCTTCTGCTGTTGAGGGAGTTATTATTTCCGACAATGTGGTCGCATCTGAAAACCTGAAGGTAAATGATCCTGAAGTATCTAAGGATCAAAAAGTCGAGATGCCGCAGAACATGACAGAAATTGAAGGCACTGTCCAGGCAGCGGGGGACGATGGTTTTGCGAAAGAGACTGCCACGACTGTTTCTGTTGATGGAGAGGTGAATTCAGCTGAAACTGCAGCAGCACAAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009870 GO:0009889 GO:0009987 GO:0010204 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0030154 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032502 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Myb_Cef Myb_DNA-bind_6 Myb_DNA-binding

Protein Analysis

959

Amino Acids

107.52

Weight (kDa)

5.13

Isoelectric Point (pI)

52.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	8 - 53	4.4e-13	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	10 - 59	1.9e-08	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	62 - 104	1e-09	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	63 - 110	5.6e-09	Myb-like DNA-binding domain
Myb_Cef	PF11831	422 - 654	1.9e-54	pre-mRNA splicing factor component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09770
fragaria_vesca	FvH4_3g19200 FvH4_3g19210 FvH4_6g01950
malus_domestica	MD04G1229500.v1.1 MD12G1246400.v1.1
prunus_persica	Prupe.6G349400_v2.0.a1
pyrus_communis	pycom04g20330 pycom04g20340 pycom12g22600
rosa_chinensis	RchiOBHm_Chr3g0449801 RchiOBHm_Chr5g0032281
rosa_laevigata	RLG00000025799 RLG00000033377
rosa_multiflora	Rmu_co8441867.1_g000001 Rmu_sc0000610.1_g000011 Rmu_sc0013983.1_g000008 Rmu_ssc0000013.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048170 Rroxscaffold_1G00048180 Rroxscaffold_6G00427620
rosa_rugosa	Rorug02G0623400 Rorug02G0623400 Rorug05G0130300
rosa_samantha	Rh3AG021600 Rh3BG022300 Rh3CG020900 Rh3DG022000 Rh5BG224900 Rh5CG250300 Rh5DG229500 Rh5DG229600
rosa_wichuraiana	Rw3G001770 Rw5G020450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2681
Acc36I	ACCTGC	1 cut(s)	415
AccB1I	GGYRCC	1 cut(s)	1256
AccBSI	CCGCTC	2 cut(s)	295, 2281
AccI	GTMKAC	1 cut(s)	1797
AccII	CGCG	1 cut(s)	466
AccIII	TCCGGA	1 cut(s)	859
AclI	AACGTT	1 cut(s)	2150
AclWI	GGATC	8 cut(s)	43, 350, 413, 851, 2313, 2450, 2705, 2736
AcoI	YGGCCR	2 cut(s)	1113, 2132
AcuI	CTGAAG	4 cut(s)	987, 999, 2720, 2736
AcyI	GRCGYC	1 cut(s)	1304
AfaI	GTAC	7 cut(s)	149, 274, 1463, 1476, 1846, 2006, 2331
AflIII	ACRYGT	1 cut(s)	1527
AgsI	TTSAA	7 cut(s)	530, 1214, 1520, 1871, 1973, 2559, 2768
AjnI	CCWGG	6 cut(s)	373, 512, 664, 1115, 1249, 2778
AloI	GAACNNNNNNTCC	2 cut(s)	2475, 2507
Alw21I	GWGCWC	3 cut(s)	472, 1003, 1620
Alw26I	GTCTC	3 cut(s)	1134, 2132, 2804
AlwI	GGATC	8 cut(s)	43, 350, 413, 851, 2313, 2450, 2705, 2736
Ama87I	CYCGRG	4 cut(s)	290, 395, 737, 996
Aor13HI	TCCGGA	1 cut(s)	859
AoxI	GGCC	5 cut(s)	371, 534, 1113, 1261, 2132
ArsI	GACNNNNNNTTYG	4 cut(s)	383, 415, 2783, 2815
Asp700I	GAANNNNTTC	2 cut(s)	1923, 2598
AspLEI	GCGC	3 cut(s)	466, 769, 1827
AspS9I	GGNCC	1 cut(s)	1838
AsuC2I	CCSGG	2 cut(s)	348, 2496
AsuHPI	GGTGA	5 cut(s)	362, 1094, 2525, 2585, 2854
AsuNHI	GCTAGC	1 cut(s)	1540
AvaI	CYCGRG	4 cut(s)	290, 395, 737, 996
AvaII	GGWCC	1 cut(s)	1838
AxyI	CCTNAGG	2 cut(s)	1506, 2409
BalI	TGGCCA	2 cut(s)	1115, 2134
BanI	GGYRCC	1 cut(s)	1256
BanII	GRGCYC	4 cut(s)	94, 472, 1003, 1620
BauI	CACGAG	1 cut(s)	2016
Bbv12I	GWGCWC	3 cut(s)	472, 1003, 1620
BbvCI	CCTCAGC	1 cut(s)	1557
BcgI	CGANNNNNNTGC	2 cut(s)	2783, 2817
BciT130I	CCWGG	6 cut(s)	375, 514, 666, 1117, 1251, 2780
BciVI	GTATCC	1 cut(s)	1788
BclI	TGATCA	1 cut(s)	12
BcnI	CCSGG	2 cut(s)	348, 2496
BcoDI	GTCTC	3 cut(s)	1134, 2132, 2804
BfmI	CTRYAG	5 cut(s)	554, 2288, 2415, 2435, 2859
BfuAI	ACCTGC	1 cut(s)	415
BfuI	GTATCC	1 cut(s)	1788
BlpI	GCTNAGC	1 cut(s)	225
BmcAI	AGTACT	1 cut(s)	1846
Bme1390I	CCNGG	8 cut(s)	348, 375, 514, 666, 1117, 1251, 2496, 2780
Bme18I	GGWCC	1 cut(s)	1838
BmeT110I	CYCGRG	4 cut(s)	290, 395, 737, 996
BmgT120I	GGNCC	1 cut(s)	1838
BmiI	GGNNCC	2 cut(s)	1258, 1819
BmrFI	CCNGG	8 cut(s)	348, 375, 514, 666, 1117, 1251, 2496, 2780
BmtI	GCTAGC	1 cut(s)	1544
BplI	GAGNNNNNCTC	2 cut(s)	1114, 1146
BpmI	CTGGAG	6 cut(s)	396, 648, 1272, 1424, 1470, 2420
Bpu10I	CCTNAGC	1 cut(s)	1557
Bpu1102I	GCTNAGC	1 cut(s)	225
BpuEI	CTTGAG	3 cut(s)	38, 2204, 2297
BpuMI	CCSGG	2 cut(s)	348, 2496
BsaBI	GATNNNNATC	1 cut(s)	11
BsaHI	GRCGYC	1 cut(s)	1304
BsaI	GGTCTC	1 cut(s)	2132
BsaJI	CCNNGG	3 cut(s)	359, 513, 1028
BsaWI	WCCGGW	1 cut(s)	859
Bse118I	RCCGGY	1 cut(s)	1259
Bse1I	ACTGG	4 cut(s)	85, 698, 1078, 1407
Bse21I	CCTNAGG	2 cut(s)	1506, 2409
Bse3DI	GCAATG	2 cut(s)	252, 1941
Bse8I	GATNNNNATC	1 cut(s)	11
BseAI	TCCGGA	1 cut(s)	859
BseBI	CCWGG	6 cut(s)	375, 514, 666, 1117, 1251, 2780
BseDI	CCNNGG	3 cut(s)	359, 513, 1028
BseJI	GATNNNNATC	1 cut(s)	11
BseMI	GCAATG	2 cut(s)	252, 1941
BseMII	CTCAG	8 cut(s)	174, 1145, 1497, 1548, 1779, 2365, 2400, 2484
BseNI	ACTGG	4 cut(s)	85, 698, 1078, 1407
BseRI	GAGGAG	3 cut(s)	651, 1164, 1523
BseYI	CCCAGC	1 cut(s)	1033
BsgI	GTGCAG	2 cut(s)	882, 2564
Bsh1236I	CGCG	1 cut(s)	466
BshFI	GGCC	5 cut(s)	373, 536, 1115, 1263, 2134
BshNI	GGYRCC	1 cut(s)	1256
BsiHKAI	GWGCWC	3 cut(s)	472, 1003, 1620
BsiHKCI	CYCGRG	4 cut(s)	290, 395, 737, 996
BsiSI	CCGG	4 cut(s)	347, 860, 1260, 2496
BslFI	GGGAC	3 cut(s)	1354, 1835, 2804
BsmAI	GTCTC	3 cut(s)	1134, 2132, 2804
BsmFI	GGGAC	3 cut(s)	1354, 1835, 2804
BsnI	GGCC	5 cut(s)	373, 536, 1115, 1263, 2134
Bso31I	GGTCTC	1 cut(s)	2132
BsoBI	CYCGRG	4 cut(s)	290, 395, 737, 996
Bsp1286I	GDGCHC	4 cut(s)	94, 472, 1003, 1620
Bsp13I	TCCGGA	1 cut(s)	859
Bsp1720I	GCTNAGC	1 cut(s)	225
BspANI	GGCC	5 cut(s)	373, 536, 1115, 1263, 2134
BspCNI	CTCAG	8 cut(s)	175, 1144, 1498, 1549, 1780, 2364, 2401, 2483
BspEI	TCCGGA	1 cut(s)	859
BspFNI	CGCG	1 cut(s)	466
BspHI	TCATGA	1 cut(s)	66
BspLI	GGNNCC	2 cut(s)	1258, 1819
BspMAI	CTGCAG	4 cut(s)	558, 2419, 2439, 2863
BspMI	ACCTGC	1 cut(s)	415
BspOI	GCTAGC	1 cut(s)	1544
BspPI	GGATC	8 cut(s)	43, 350, 413, 851, 2313, 2450, 2705, 2736
BspQI	GCTCTTC	1 cut(s)	1008
BspT107I	GGYRCC	1 cut(s)	1256
BspTNI	GGTCTC	1 cut(s)	2132
BsrBI	CCGCTC	2 cut(s)	295, 2281
BsrDI	GCAATG	2 cut(s)	252, 1941
BsrFI	RCCGGY	1 cut(s)	1259
BsrI	ACTGG	4 cut(s)	85, 698, 1078, 1407
BssAI	RCCGGY	1 cut(s)	1259
BssECI	CCNNGG	3 cut(s)	359, 513, 1028
BssNI	GRCGYC	1 cut(s)	1304
BssSI	CACGAG	1 cut(s)	2016
BssT1I	CCWWGG	1 cut(s)	1028
Bst2BI	CACGAG	1 cut(s)	2016
Bst2UI	CCWGG	6 cut(s)	375, 514, 666, 1117, 1251, 2780
Bst4CI	ACNGT	6 cut(s)	132, 868, 1849, 2431, 2776, 2824
Bst6I	CTCTTC	7 cut(s)	192, 525, 740, 869, 1008, 1515, 1586
BstACI	GRCGYC	1 cut(s)	1304
BstDSI	CCRYGG	1 cut(s)	359
BstENI	CCTNNNNNAGG	1 cut(s)	663
BstFNI	CGCG	1 cut(s)	466
BstHHI	GCGC	3 cut(s)	466, 769, 1827
BstMAI	GTCTC	3 cut(s)	1134, 2132, 2804
BstNI	CCWGG	6 cut(s)	375, 514, 666, 1117, 1251, 2780
BstNSI	RCATGY	1 cut(s)	1531
BstSCI	CCNGG	8 cut(s)	346, 373, 512, 664, 1115, 1249, 2494, 2778
BstSFI	CTRYAG	5 cut(s)	554, 2288, 2415, 2435, 2859
BstUI	CGCG	1 cut(s)	466
BstX2I	RGATCY	2 cut(s)	48, 2305
BstYI	RGATCY	2 cut(s)	48, 2305
Bsu36I	CCTNAGG	2 cut(s)	1506, 2409
BsuI	GTATCC	1 cut(s)	1788
BsuRI	GGCC	5 cut(s)	373, 536, 1115, 1263, 2134
BtgI	CCRYGG	1 cut(s)	359
BtgZI	GCGATG	1 cut(s)	2621
BtsI	GCAGTG	2 cut(s)	290, 1834
BtsIMutI	CAGTG	6 cut(s)	92, 137, 290, 705, 1834, 2772
BveI	ACCTGC	1 cut(s)	415
CciI	TCATGA	1 cut(s)	66
CfoI	GCGC	3 cut(s)	466, 769, 1827
Cfr10I	RCCGGY	1 cut(s)	1259
Cfr13I	GGNCC	1 cut(s)	1838
CseI	GACGC	1 cut(s)	1312
Csp6I	GTAC	7 cut(s)	148, 273, 1462, 1475, 1845, 2005, 2330
CspCI	CAANNNNNGTGG	2 cut(s)	348, 383
CviQI	GTAC	7 cut(s)	148, 273, 1462, 1475, 1845, 2005, 2330
DraI	TTTAAA	1 cut(s)	2194
DrdI	GACNNNNNNGTC	1 cut(s)	2681
DseDI	GACNNNNNNGTC	1 cut(s)	2681
EaeI	YGGCCR	2 cut(s)	1113, 2132
Eam1104I	CTCTTC	7 cut(s)	192, 525, 740, 869, 1008, 1515, 1586
EarI	CTCTTC	7 cut(s)	192, 525, 740, 869, 1008, 1515, 1586
Ecl136II	GAGCTC	3 cut(s)	470, 1001, 1618
Eco130I	CCWWGG	1 cut(s)	1028
Eco24I	GRGCYC	4 cut(s)	94, 472, 1003, 1620
Eco31I	GGTCTC	1 cut(s)	2132
Eco32I	GATATC	1 cut(s)	2053
Eco47I	GGWCC	1 cut(s)	1838
Eco53kI	GAGCTC	3 cut(s)	470, 1001, 1618
Eco57I	CTGAAG	4 cut(s)	987, 999, 2720, 2736
Eco81I	CCTNAGG	2 cut(s)	1506, 2409
Eco88I	CYCGRG	4 cut(s)	290, 395, 737, 996
EcoICRI	GAGCTC	3 cut(s)	470, 1001, 1618
EcoNI	CCTNNNNNAGG	1 cut(s)	663
EcoRI	GAATTC	2 cut(s)	2389, 2845
EcoRII	CCWGG	6 cut(s)	373, 512, 664, 1115, 1249, 2778
EcoRV	GATATC	1 cut(s)	2053
EcoT14I	CCWWGG	1 cut(s)	1028
EcoT38I	GRGCYC	4 cut(s)	94, 472, 1003, 1620
ErhI	CCWWGG	1 cut(s)	1028
FalI	AAGNNNNNCTT	8 cut(s)	532, 564, 1303, 1335, 1569, 1601, 1728, 1760
FaqI	GGGAC	3 cut(s)	1354, 1835, 2804
FauI	CCCGC	2 cut(s)	2106, 2779
FauNDI	CATATG	1 cut(s)	2098
FbaI	TGATCA	1 cut(s)	12
FblI	GTMKAC	1 cut(s)	1797
FriOI	GRGCYC	4 cut(s)	94, 472, 1003, 1620
GlaI	GCGC	3 cut(s)	465, 768, 1826
GsaI	CCCAGC	1 cut(s)	1037
GsuI	CTGGAG	6 cut(s)	396, 648, 1272, 1424, 1470, 2420
HaeIII	GGCC	5 cut(s)	373, 536, 1115, 1263, 2134
HapII	CCGG	4 cut(s)	347, 860, 1260, 2496
HgaI	GACGC	1 cut(s)	1312
HhaI	GCGC	3 cut(s)	466, 769, 1827
Hin1I	GRCGYC	1 cut(s)	1304
Hin6I	GCGC	3 cut(s)	464, 767, 1825
HinP1I	GCGC	3 cut(s)	464, 767, 1825
HindIII	AAGCTT	2 cut(s)	209, 2196
HinfI	GANTC	9 cut(s)	96, 398, 1127, 1267, 1316, 1342, 1895, 1952, 2263
HpaII	CCGG	4 cut(s)	347, 860, 1260, 2496
HphI	GGTGA	5 cut(s)	362, 1094, 2525, 2585, 2854
Hpy166II	GTNNAC	4 cut(s)	189, 1442, 1798, 2626
Hpy8I	GTNNAC	4 cut(s)	189, 1442, 1798, 2626
Hpy99I	CGWCG	2 cut(s)	106, 283
HpyCH4III	ACNGT	6 cut(s)	132, 868, 1849, 2431, 2776, 2824
HpyCH4IV	ACGT	1 cut(s)	2150
HpySE526I	ACGT	1 cut(s)	2150
Hsp92I	GRCGYC	1 cut(s)	1304
HspAI	GCGC	3 cut(s)	464, 767, 1825
Kpn2I	TCCGGA	1 cut(s)	859
KroI	GCCGGC	1 cut(s)	1259
KroNI	GCCGGC	1 cut(s)	1261
Ksp22I	TGATCA	1 cut(s)	12
LguI	GCTCTTC	1 cut(s)	1008
LmnI	GCTCC	8 cut(s)	703, 826, 1405, 1489, 1615, 1623, 2021, 2272
MaeII	ACGT	1 cut(s)	2150
MaeIII	GTNAC	3 cut(s)	1189, 2425, 2461
MbiI	CCGCTC	2 cut(s)	295, 2281
MflI	RGATCY	2 cut(s)	48, 2305
MhlI	GDGCHC	4 cut(s)	94, 472, 1003, 1620
MlsI	TGGCCA	2 cut(s)	1115, 2134
MluNI	TGGCCA	2 cut(s)	1115, 2134
MlyI	GAGTC	4 cut(s)	407, 1136, 1261, 1336
MmeI	TCCRAC	2 cut(s)	903, 2697
Mox20I	TGGCCA	2 cut(s)	1115, 2134
MroI	TCCGGA	1 cut(s)	859
MroNI	GCCGGC	1 cut(s)	1259
MroXI	GAANNNNTTC	2 cut(s)	1923, 2598
MscI	TGGCCA	2 cut(s)	1115, 2134
MseI	TTAA	6 cut(s)	327, 572, 1734, 2085, 2154, 2193
MslI	CAYNNNNRTG	1 cut(s)	1767
Msp20I	TGGCCA	2 cut(s)	1115, 2134
MspA1I	CMGCKG	5 cut(s)	965, 2171, 2615, 2786, 2852
MspI	CCGG	4 cut(s)	347, 860, 1260, 2496
MspR9I	CCNGG	8 cut(s)	348, 375, 514, 666, 1117, 1251, 2496, 2780
MvaI	CCWGG	6 cut(s)	375, 514, 666, 1117, 1251, 2780
MvnI	CGCG	1 cut(s)	466
NaeI	GCCGGC	1 cut(s)	1261
NciI	CCSGG	2 cut(s)	348, 2496
NdeI	CATATG	1 cut(s)	2098
NgoMIV	GCCGGC	1 cut(s)	1259
NheI	GCTAGC	1 cut(s)	1540
NlaIV	GGNNCC	2 cut(s)	1258, 1819
NmuCI	GTSAC	2 cut(s)	1189, 2425
NspI	RCATGY	1 cut(s)	1531
PaeR7I	CTCGAG	3 cut(s)	290, 737, 996
PagI	TCATGA	1 cut(s)	66
PciI	ACATGT	1 cut(s)	1527
PciSI	GCTCTTC	1 cut(s)	1008
PcsI	WCGNNNNNNNCGW	1 cut(s)	156
PdiI	GCCGGC	1 cut(s)	1261
PdmI	GAANNNNTTC	2 cut(s)	1923, 2598
PfeI	GAWTC	5 cut(s)	96, 1316, 1895, 1952, 2263
PfoI	TCCNGGA	1 cut(s)	1249
PleI	GAGTC	4 cut(s)	406, 1135, 1261, 1336
PpsI	GAGTC	4 cut(s)	406, 1135, 1261, 1336
PscI	ACATGT	1 cut(s)	1527
Psp124BI	GAGCTC	3 cut(s)	472, 1003, 1620
Psp1406I	AACGTT	1 cut(s)	2150
Psp6I	CCWGG	6 cut(s)	373, 512, 664, 1115, 1249, 2778
PspFI	CCCAGC	1 cut(s)	1033
PspGI	CCWGG	6 cut(s)	373, 512, 664, 1115, 1249, 2778
PspN4I	GGNNCC	2 cut(s)	1258, 1819
PspPI	GGNCC	1 cut(s)	1838
PspXI	VCTCGAGB	3 cut(s)	290, 737, 996
PsrI	GAACNNNNNNTAC	2 cut(s)	1267, 1299
PstI	CTGCAG	4 cut(s)	558, 2419, 2439, 2863
PsuI	RGATCY	2 cut(s)	48, 2305
PvuII	CAGCTG	2 cut(s)	965, 2852
RsaI	GTAC	7 cut(s)	149, 274, 1463, 1476, 1846, 2006, 2331
RsaNI	GTAC	7 cut(s)	148, 273, 1462, 1475, 1845, 2005, 2330
RseI	CAYNNNNRTG	1 cut(s)	1767
SacI	GAGCTC	3 cut(s)	472, 1003, 1620
SapI	GCTCTTC	1 cut(s)	1008
SaqAI	TTAA	6 cut(s)	327, 572, 1734, 2085, 2154, 2193
Sau96I	GGNCC	1 cut(s)	1838
ScaI	AGTACT	1 cut(s)	1846
SchI	GAGTC	4 cut(s)	407, 1136, 1261, 1336
ScrFI	CCNGG	8 cut(s)	348, 375, 514, 666, 1117, 1251, 2496, 2780
SduI	GDGCHC	4 cut(s)	94, 472, 1003, 1620
SfcI	CTRYAG	5 cut(s)	554, 2288, 2415, 2435, 2859
Sfr274I	CTCGAG	3 cut(s)	290, 737, 996
SinI	GGWCC	1 cut(s)	1838
SlaI	CTCGAG	3 cut(s)	290, 737, 996
SmiMI	CAYNNNNRTG	1 cut(s)	1767
SmlI	CTYRAG	6 cut(s)	53, 290, 737, 996, 2183, 2276
SmoI	CTYRAG	6 cut(s)	53, 290, 737, 996, 2183, 2276
SstI	GAGCTC	3 cut(s)	472, 1003, 1620
StyD4I	CCNGG	8 cut(s)	346, 373, 512, 664, 1115, 1249, 2494, 2778
StyI	CCWWGG	1 cut(s)	1028
TaaI	ACNGT	6 cut(s)	132, 868, 1849, 2431, 2776, 2824
TaiI	ACGT	1 cut(s)	2153
TaqI	TCGA	8 cut(s)	94, 159, 291, 401, 738, 997, 2316, 2739
TatI	WGTACW	2 cut(s)	1844, 2004
TauI	GCSGC	5 cut(s)	62, 373, 2225, 2284, 2749
TfiI	GAWTC	5 cut(s)	96, 1316, 1895, 1952, 2263
Tru1I	TTAA	6 cut(s)	327, 572, 1734, 2085, 2154, 2193
Tru9I	TTAA	6 cut(s)	327, 572, 1734, 2085, 2154, 2193
TscAI	CASTG	6 cut(s)	92, 137, 290, 705, 1834, 2779
TseFI	GTSAC	2 cut(s)	1189, 2425
Tsp45I	GTSAC	2 cut(s)	1189, 2425
TspGWI	ACGGA	3 cut(s)	53, 267, 376
TspRI	CASTG	6 cut(s)	92, 137, 290, 705, 1834, 2779
VpaK11BI	GGWCC	1 cut(s)	1838
XagI	CCTNNNNNAGG	1 cut(s)	663
XceI	RCATGY	1 cut(s)	1531
XhoI	CTCGAG	3 cut(s)	290, 737, 996
XmiI	GTMKAC	1 cut(s)	1797
XmnI	GAANNNNTTC	2 cut(s)	1923, 2598
ZrmI	AGTACT	1 cut(s)	1846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.