Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0003901

Physical Location & Seq

Genomic Coordinates Forward (+)

23449466 .. 23453955

Gene Structure 4490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g22600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2937 bp

ATGAGGATTATGATCAAAGGAGGCGTGTGGAAGAACACGGAGGATGAGATCCTCAAGGCGGCGGTCATGAAGTACGGGAAGAACCAGTGGGCTCGAATCTCGTCGCTCTTGGTTCGCAAATCGGCTAAGCAGTGTAAGGCGAGATGGTACGAGTGGCTCGACCCCTCCATCAAAAAGACTGAGTGGACTAGAGAAGAGGATGAGAAGCTTCTGCATCTTGCTAAGCTCATGCCTACACAATGGAGGACCATTGCCCCAATTGTTGGTCGTACTCCGTCGCAGTGCCTCGAGCGGTATGAGAAGTTGCTTGATGCAGCCTGTGTTAAGGATGAGAATTATGAACCGGGTGATGATCCACGGAAACTGCGGCCTGGAGAGATTGACCCAAATCCCGAGTCGAAGCCTGCTCGTCCTGATCCTGTTGATATGGATGAGGATGAGAAGGAGATGCTTTCAGAGGCACGAGCTCGGTTAGCAAACACTAGGGGAAAGAAAGCTAAAAGGAAAGCCAGGGAGAAACAGCTTGAAGAGGCCAGAAGGCTTGCTTCGCTGCAGAAAAGGAGAGAATTAAAGGCTGCTGGCATTGATACTAGGCAGCGAAAGAGGAAAAGGAAGGGAATAGACTACAATGCGGAAATTCCCTTTGAGAAGAAGCCTCCTCCAGGCTTTTATGATGTTGCTGATGAAGATAGACCAGTGGAGCAGCCTAACTTCCCTACGACTATTGAGGAACTTGAGGGAAAGAGAAGGATTGATGTGGAAGCGCAGTTAAGAAAGCAAGATATTGCTAAGAACAAAATTGCTCAAAGGCAGGATGCTCCATCAGCTATACTGCAAGCCAACAAGATGAATGATCCGGAAACAGTTAGGAAGAGATCAAAACTGATGCTTCCTGCACCCCAAATTTCGGACAATGAATTGGAGGAAATTGCAAAGATGGGTTATGCTAATGATCTTGCAGTTGAAGAATTCGCTGAAGGTAGCGGTGCAACTCGAGCTCTTCTTGCAAATTATTCCCAGACACCAAGGCTAGGAATGACACCTTCACGAACACCTCAAAGAACACCTGCTGGTAAGGGTGATGCTATCATGATGGAAGCAGAAAATTTGGCCAGGATGAGAGAGTCACAGACACCATTATTAGGAGGAGATAATCCAGAGTTACACCCATCAGATTTCTCGGGGGTCACTCCTAGAAAAAAGGAAATTCAAACGCCAAATCTGATGCTTACTCCTTCTGCAACTCCTGGAGGTGGTCTTACACCTAGAACAGGTCTTACGCCTAGAGTTGGCATGACGCCATCGAGGGATTCTTTTGGCATGACCCCAAAGGGGACTCCCATTAGGGATGAGCTTCGTATCAATGAAGAAATAGATACACCTAATAGTGCAAAACTGGAGCAGCGTAGAAACTTGCAGTTTGGTTTTGGCAGTTTACCACAGCCTAAGAATGAGTACCAGATTGTCGTACAACCAGTTCCAGAGGATACTGAAGAACCTGAGGAGAAGATTGAAGAAGACATGTCTGATAGGCTAGCCAGAGAAAGGGCCGAGGAAGAAGCACGCCAGCAAGCATTACTTAGGAAGAGATCGAAAGTATTGCAGAGGGAGCTCCCTAGACCTCCTGCTGCCTCATTGGATCTCATCAGAAATTCCTTGATAAAAGCAGATGGAGATAGGAGTTCATTTGTTCCTCCTACTCCTATTGAGCAGGCTGATGAAATGGTTAAAGTAGAACTTCTTAGCCTACTAGAGCATGATAATGCCAAGTATCCATTTACTGAGAAAGTAGACAAACGGAAAAAGAAAGGTTCCAAGCGCAGTGCCAATGGACCTACTACTTCTGTCCCTGTGATTGAAGATTTTGAAGAAGATGAACTGAAAGAGGCTGATTCATTGATAAAGGAAGATGCAGAAATTCTTCGTGTTGCAATGAGGCATGAAAACGAATCCCTTGATGAATTTGTTGAAGCACACAAAACTTGCCTGATCGATCTCATGTACTTCCCTACTCGTGGCGCTTATGGTCTATCAAGTGTTGCCGGAAATATGGAGAAGCTTGCAGCTTTACAGAGTGAGTTTGAGAATGTGAAGAAGAAGATGGATGATGATATCCAGAAGGCAGCAAGCATTGAGAACAAGGTTAAAGTTCGCACATATGGTTATGAGATGCGGGCTAAAGAGGGACTCTGGCCAAAAATTGAGGAAACGTTTAAGCAGATTGACACTGCTGCAAAAGAACTTGAGTGCTTTAAAGCTTTACAGAAGCAAGAAAGATTAGCGGCAACACACCGGATAAATAATATCTGGGAGGAAGTTCAGAATCAAAAGGAGCTTGAGAGGCAACTACAGAAGCGGTATGGCAATCTTGTAGTTGAGGTGGAAAGGGGACAGCGGCGCATGGATGAACTCAGAGCAGAAGCAGAAAAACAAGAAATTGCTGCCAGGAATTCTGATCTGGAGTTGGCTGAGGCTACAGCAAATGTGACTGTTCAACAGACAACTGAAAATCCTGATCCTGTAACTGCTTCAGACGAGCCTGGAAATGCTGTTCCCGGTGGTGCATCTGATGGTGAAGCAACAAATCAACAAATGGATGGTGTGCAAGAACAGGCTTCAACAGGCCCAGAAGGTGATATGGACATTGATGCCAAAAAGGTTCATGCGACGCCAGCAGATGATGTAAACTTACCTGATAACATGCCTTCTGCTGTTGAGGGAGATAACGTTTCCGACAATGTGGTTGCATCTGAAAACCTGAAGGTAAATGATCCTGATGTATCTAAGGATCAAAAAGTTGAGATGCCACAGAACGTGACAGAAATTGAAGGCACTGTCCAAGCAGCGGGGGACGATGGTTTTGCTAACGGGACTGCCGAGATTGTTTCTGTTGAGGGAGAGGTGAATTCAACTGAAAATGCAGCACCACAAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009870 GO:0009889 GO:0009987 GO:0010204 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0030154 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032502 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Myb_Cef Myb_DNA-bind_6 Myb_DNA-binding

Protein Analysis

979

Amino Acids

109.75

Weight (kDa)

5.18

Isoelectric Point (pI)

51.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	8 - 53	4.6e-13	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	10 - 70	7.7e-14	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	59 - 103	6.1e-10	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	62 - 109	5.7e-09	Myb-like DNA-binding domain
Myb_Cef	PF11831	420 - 653	1.9e-55	pre-mRNA splicing factor component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09770
fragaria_vesca	FvH4_3g19200 FvH4_3g19210 FvH4_6g01950
malus_domestica	MD04G1229500.v1.1 MD12G1246400.v1.1
prunus_persica	Prupe.6G349400_v2.0.a1
pyrus_communis	pycom04g20330 pycom04g20340 pycom12g22600
rosa_chinensis	RchiOBHm_Chr3g0449801 RchiOBHm_Chr5g0032281
rosa_laevigata	RLG00000025799 RLG00000033377
rosa_multiflora	Rmu_co8441867.1_g000001 Rmu_sc0000610.1_g000011 Rmu_sc0013983.1_g000008 Rmu_ssc0000013.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048170 Rroxscaffold_1G00048180 Rroxscaffold_6G00427620
rosa_rugosa	Rorug02G0623400 Rorug02G0623400 Rorug05G0130300
rosa_samantha	Rh3AG021600 Rh3BG022300 Rh3CG020900 Rh3DG022000 Rh5BG224900 Rh5CG250300 Rh5DG229500 Rh5DG229600
rosa_wichuraiana	Rw3G001770 Rw5G020450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1075
Acc36I	ACCTGC	1 cut(s)	1075
AccB7I	CCANNNNNTGG	1 cut(s)	263
AccBSI	CCGCTC	1 cut(s)	292
AccI	GTMKAC	1 cut(s)	1791
AccIII	TCCGGA	1 cut(s)	856
AclI	AACGTT	2 cut(s)	2210, 2727
AclWI	GGATC	8 cut(s)	43, 347, 410, 848, 1647, 2510, 2765, 2796
AcoI	YGGCCR	2 cut(s)	1110, 2192
AcuI	CTGAAG	4 cut(s)	996, 1512, 2514, 2780
AcyI	GRCGYC	2 cut(s)	1298, 2669
AfaI	GTAC	6 cut(s)	74, 149, 271, 1457, 1470, 2003
AflIII	ACRYGT	1 cut(s)	1521
AjnI	CCWGG	7 cut(s)	370, 509, 661, 1112, 1246, 2444, 2539
AloI	GAACNNNNNNTCC	2 cut(s)	2535, 2567
Alw21I	GWGCWC	3 cut(s)	469, 1000, 1614
AlwI	GGATC	8 cut(s)	43, 347, 410, 848, 1647, 2510, 2765, 2796
Ama87I	CYCGRG	4 cut(s)	287, 392, 993, 1180
Aor13HI	TCCGGA	1 cut(s)	856
AoxI	GGCC	6 cut(s)	368, 531, 1110, 1548, 2192, 2623
ArsI	GACNNNNNNTTYG	4 cut(s)	380, 412, 2843, 2875
Asp700I	GAANNNNTTC	1 cut(s)	1920
AspLEI	GCGC	4 cut(s)	766, 1821, 2021, 2400
AspS9I	GGNCC	4 cut(s)	246, 1548, 1832, 2624
AsuC2I	CCSGG	2 cut(s)	345, 2556
AsuHPI	GGTGA	5 cut(s)	359, 1091, 2585, 2645, 2914
AsuNHI	GCTAGC	1 cut(s)	1534
AvaI	CYCGRG	4 cut(s)	287, 392, 993, 1180
AvaII	GGWCC	2 cut(s)	246, 1832
AxyI	CCTNAGG	1 cut(s)	1500
BalI	TGGCCA	2 cut(s)	1112, 2194
BanII	GRGCYC	4 cut(s)	94, 469, 1000, 1614
BauI	CACGAG	2 cut(s)	462, 2013
BbsI	GAAGAC	1 cut(s)	1524
Bbv12I	GWGCWC	3 cut(s)	469, 1000, 1614
BbvCI	CCTCAGC	1 cut(s)	2469
BcgI	CGANNNNNNTGC	4 cut(s)	1582, 1616, 2843, 2877
BciT130I	CCWGG	7 cut(s)	372, 511, 663, 1114, 1248, 2446, 2541
BciVI	GTATCC	2 cut(s)	1480, 1782
BclI	TGATCA	1 cut(s)	12
BcnI	CCSGG	2 cut(s)	345, 2556
BfmI	CTRYAG	3 cut(s)	551, 2348, 2475
BfoI	RGCGCY	1 cut(s)	2022
BfuAI	ACCTGC	1 cut(s)	1075
BfuI	GTATCC	2 cut(s)	1480, 1782
BlpI	GCTNAGC	2 cut(s)	126, 222
Bme1390I	CCNGG	9 cut(s)	345, 372, 511, 663, 1114, 1248, 2446, 2541, 2556
Bme18I	GGWCC	2 cut(s)	246, 1832
BmeT110I	CYCGRG	4 cut(s)	287, 392, 993, 1180
BmgT120I	GGNCC	4 cut(s)	246, 1548, 1832, 2624
BmiI	GGNNCC	1 cut(s)	1813
BmrFI	CCNGG	9 cut(s)	345, 372, 511, 663, 1114, 1248, 2446, 2541, 2556
BmtI	GCTAGC	1 cut(s)	1538
BpiI	GAAGAC	1 cut(s)	1524
BpmI	CTGGAG	5 cut(s)	393, 645, 1269, 1418, 2480
Bpu10I	CCTNAGC	1 cut(s)	2469
Bpu1102I	GCTNAGC	2 cut(s)	126, 222
BpuEI	CTTGAG	4 cut(s)	38, 755, 2264, 2357
BpuMI	CCSGG	2 cut(s)	345, 2556
Bsa29I	ATCGAT	1 cut(s)	1992
BsaBI	GATNNNNATC	1 cut(s)	11
BsaHI	GRCGYC	2 cut(s)	1298, 2669
BsaJI	CCNNGG	4 cut(s)	356, 510, 1025, 1551
BsaWI	WCCGGW	2 cut(s)	856, 2292
BsaXI	ACNNNNNCTCC	2 cut(s)	2712, 2742
Bse1I	ACTGG	4 cut(s)	85, 695, 1401, 1475
Bse21I	CCTNAGG	1 cut(s)	1500
Bse3DI	GCAATG	2 cut(s)	249, 1938
Bse8I	GATNNNNATC	1 cut(s)	11
BseAI	TCCGGA	1 cut(s)	856
BseBI	CCWGG	7 cut(s)	372, 511, 663, 1114, 1248, 2446, 2541
BseCI	ATCGAT	1 cut(s)	1992
BseDI	CCNNGG	4 cut(s)	356, 510, 1025, 1551
BseJI	GATNNNNATC	1 cut(s)	11
BseMI	GCAATG	2 cut(s)	249, 1938
BseMII	CTCAG	5 cut(s)	171, 1491, 1773, 2425, 2460
BseNI	ACTGG	4 cut(s)	85, 695, 1401, 1475
BseRI	GAGGAG	3 cut(s)	648, 1161, 1517
BsgI	GTGCAG	1 cut(s)	879
BshFI	GGCC	6 cut(s)	370, 533, 1112, 1550, 2194, 2625
BshVI	ATCGAT	1 cut(s)	1992
BsiHKAI	GWGCWC	3 cut(s)	469, 1000, 1614
BsiHKCI	CYCGRG	4 cut(s)	287, 392, 993, 1180
BsiSI	CCGG	5 cut(s)	344, 857, 2043, 2293, 2556
BslFI	GGGAC	6 cut(s)	1348, 1832, 2199, 2403, 2864, 2884
BsmFI	GGGAC	6 cut(s)	1348, 1832, 2199, 2403, 2864, 2884
BsnI	GGCC	6 cut(s)	370, 533, 1112, 1550, 2194, 2625
BsoBI	CYCGRG	4 cut(s)	287, 392, 993, 1180
Bsp1286I	GDGCHC	4 cut(s)	94, 469, 1000, 1614
Bsp13I	TCCGGA	1 cut(s)	856
Bsp1720I	GCTNAGC	2 cut(s)	126, 222
BspANI	GGCC	6 cut(s)	370, 533, 1112, 1550, 2194, 2625
BspCNI	CTCAG	5 cut(s)	172, 1492, 1774, 2424, 2461
BspDI	ATCGAT	1 cut(s)	1992
BspEI	TCCGGA	1 cut(s)	856
BspHI	TCATGA	2 cut(s)	66, 1089
BspLI	GGNNCC	1 cut(s)	1813
BspMAI	CTGCAG	1 cut(s)	555
BspMI	ACCTGC	1 cut(s)	1075
BspOI	GCTAGC	1 cut(s)	1538
BspPI	GGATC	8 cut(s)	43, 347, 410, 848, 1647, 2510, 2765, 2796
BspQI	GCTCTTC	1 cut(s)	1005
BsrBI	CCGCTC	1 cut(s)	292
BsrDI	GCAATG	2 cut(s)	249, 1938
BsrI	ACTGG	4 cut(s)	85, 695, 1401, 1475
BssECI	CCNNGG	4 cut(s)	356, 510, 1025, 1551
BssNI	GRCGYC	2 cut(s)	1298, 2669
BssSI	CACGAG	2 cut(s)	462, 2013
BssT1I	CCWWGG	1 cut(s)	1025
Bst2BI	CACGAG	2 cut(s)	462, 2013
Bst2UI	CCWGG	7 cut(s)	372, 511, 663, 1114, 1248, 2446, 2541
Bst4CI	ACNGT	3 cut(s)	865, 2491, 2836
Bst6I	CTCTTC	5 cut(s)	189, 522, 866, 1005, 1580
BstACI	GRCGYC	2 cut(s)	1298, 2669
BstDSI	CCRYGG	1 cut(s)	356
BstENI	CCTNNNNNAGG	2 cut(s)	660, 1269
BstH2I	RGCGCY	1 cut(s)	2022
BstHHI	GCGC	4 cut(s)	766, 1821, 2021, 2400
BstMWI	GCNNNNNNNGC	8 cut(s)	473, 547, 824, 995, 1004, 1609, 2341, 2672
BstNI	CCWGG	7 cut(s)	372, 511, 663, 1114, 1248, 2446, 2541
BstNSI	RCATGY	2 cut(s)	1525, 2704
BstSCI	CCNGG	9 cut(s)	343, 370, 509, 661, 1112, 1246, 2444, 2539, 2554
BstSFI	CTRYAG	3 cut(s)	551, 2348, 2475
BstV2I	GAAGAC	1 cut(s)	1524
BstX2I	RGATCY	2 cut(s)	48, 1639
BstYI	RGATCY	2 cut(s)	48, 1639
Bsu15I	ATCGAT	1 cut(s)	1992
Bsu36I	CCTNAGG	1 cut(s)	1500
BsuI	GTATCC	2 cut(s)	1480, 1782
BsuRI	GGCC	6 cut(s)	370, 533, 1112, 1550, 2194, 2625
BsuTUI	ATCGAT	1 cut(s)	1992
BtgI	CCRYGG	1 cut(s)	356
BtsI	GCAGTG	4 cut(s)	137, 287, 1828, 2226
BtsIMutI	CAGTG	7 cut(s)	92, 137, 287, 702, 1828, 2226, 2832
BveI	ACCTGC	1 cut(s)	1075
CciI	TCATGA	2 cut(s)	66, 1089
CfoI	GCGC	4 cut(s)	766, 1821, 2021, 2400
Cfr13I	GGNCC	4 cut(s)	246, 1548, 1832, 2624
ClaI	ATCGAT	1 cut(s)	1992
CseI	GACGC	2 cut(s)	1306, 2677
Csp6I	GTAC	6 cut(s)	73, 148, 270, 1456, 1469, 2002
CviQI	GTAC	6 cut(s)	73, 148, 270, 1456, 1469, 2002
DraI	TTTAAA	1 cut(s)	2254
EaeI	YGGCCR	2 cut(s)	1110, 2192
Eam1104I	CTCTTC	5 cut(s)	189, 522, 866, 1005, 1580
EarI	CTCTTC	5 cut(s)	189, 522, 866, 1005, 1580
Ecl136II	GAGCTC	3 cut(s)	467, 998, 1612
Eco130I	CCWWGG	1 cut(s)	1025
Eco24I	GRGCYC	4 cut(s)	94, 469, 1000, 1614
Eco32I	GATATC	1 cut(s)	2113
Eco47I	GGWCC	2 cut(s)	246, 1832
Eco53kI	GAGCTC	3 cut(s)	467, 998, 1612
Eco57I	CTGAAG	4 cut(s)	996, 1512, 2514, 2780
Eco81I	CCTNAGG	1 cut(s)	1500
Eco88I	CYCGRG	4 cut(s)	287, 392, 993, 1180
EcoICRI	GAGCTC	3 cut(s)	467, 998, 1612
EcoNI	CCTNNNNNAGG	2 cut(s)	660, 1269
EcoRI	GAATTC	3 cut(s)	968, 2449, 2905
EcoRII	CCWGG	7 cut(s)	370, 509, 661, 1112, 1246, 2444, 2539
EcoRV	GATATC	1 cut(s)	2113
EcoT14I	CCWWGG	1 cut(s)	1025
EcoT38I	GRGCYC	4 cut(s)	94, 469, 1000, 1614
ErhI	CCWWGG	1 cut(s)	1025
FalI	AAGNNNNNCTT	6 cut(s)	529, 561, 1563, 1595, 1722, 1754
FaqI	GGGAC	6 cut(s)	1348, 1832, 2199, 2403, 2864, 2884
FauI	CCCGC	2 cut(s)	2166, 2839
FauNDI	CATATG	1 cut(s)	2158
FbaI	TGATCA	1 cut(s)	12
FblI	GTMKAC	1 cut(s)	1791
FriOI	GRGCYC	4 cut(s)	94, 469, 1000, 1614
GlaI	GCGC	4 cut(s)	765, 1820, 2020, 2399
GsuI	CTGGAG	5 cut(s)	393, 645, 1269, 1418, 2480
HaeII	RGCGCY	1 cut(s)	2022
HaeIII	GGCC	6 cut(s)	370, 533, 1112, 1550, 2194, 2625
HapII	CCGG	5 cut(s)	344, 857, 2043, 2293, 2556
HgaI	GACGC	2 cut(s)	1306, 2677
HhaI	GCGC	4 cut(s)	766, 1821, 2021, 2400
Hin1I	GRCGYC	2 cut(s)	1298, 2669
Hin6I	GCGC	4 cut(s)	764, 1819, 2019, 2398
HinP1I	GCGC	4 cut(s)	764, 1819, 2019, 2398
HindIII	AAGCTT	3 cut(s)	206, 2057, 2256
HinfI	GANTC	9 cut(s)	96, 395, 1124, 1310, 1336, 1892, 1949, 2187, 2323
HpaII	CCGG	5 cut(s)	344, 857, 2043, 2293, 2556
HphI	GGTGA	5 cut(s)	359, 1091, 2585, 2645, 2914
Hpy166II	GTNNAC	4 cut(s)	186, 1436, 1792, 2686
Hpy8I	GTNNAC	4 cut(s)	186, 1436, 1792, 2686
Hpy99I	CGWCG	3 cut(s)	106, 280, 2671
HpyCH4III	ACNGT	3 cut(s)	865, 2491, 2836
HpyCH4IV	ACGT	3 cut(s)	2210, 2727, 2814
HpyF10VI	GCNNNNNNNGC	8 cut(s)	473, 547, 824, 995, 1004, 1609, 2341, 2672
HpySE526I	ACGT	3 cut(s)	2210, 2727, 2814
Hsp92I	GRCGYC	2 cut(s)	1298, 2669
HspAI	GCGC	4 cut(s)	764, 1819, 2019, 2398
Kpn2I	TCCGGA	1 cut(s)	856
Ksp22I	TGATCA	1 cut(s)	12
LguI	GCTCTTC	1 cut(s)	1005
LmnI	GCTCC	6 cut(s)	700, 823, 1399, 1609, 1617, 2332
MaeII	ACGT	3 cut(s)	2210, 2727, 2814
MaeIII	GTNAC	6 cut(s)	1125, 1161, 1186, 2485, 2521, 2815
MbiI	CCGCTC	1 cut(s)	292
MfeI	CAATTG	1 cut(s)	258
MflI	RGATCY	2 cut(s)	48, 1639
MhlI	GDGCHC	4 cut(s)	94, 469, 1000, 1614
MlsI	TGGCCA	2 cut(s)	1112, 2194
MluNI	TGGCCA	2 cut(s)	1112, 2194
MlyI	GAGTC	4 cut(s)	404, 1133, 1330, 2181
MmeI	TCCRAC	1 cut(s)	2757
Mox20I	TGGCCA	2 cut(s)	1112, 2194
MroI	TCCGGA	1 cut(s)	856
MroXI	GAANNNNTTC	1 cut(s)	1920
MscI	TGGCCA	2 cut(s)	1112, 2194
MseI	TTAA	7 cut(s)	324, 569, 770, 1728, 2145, 2214, 2253
MslI	CAYNNNNRTG	1 cut(s)	1761
Msp20I	TGGCCA	2 cut(s)	1112, 2194
MspA1I	CMGCKG	2 cut(s)	2395, 2846
MspI	CCGG	5 cut(s)	344, 857, 2043, 2293, 2556
MspR9I	CCNGG	9 cut(s)	345, 372, 511, 663, 1114, 1248, 2446, 2541, 2556
MunI	CAATTG	1 cut(s)	258
MvaI	CCWGG	7 cut(s)	372, 511, 663, 1114, 1248, 2446, 2541
MwoI	GCNNNNNNNGC	8 cut(s)	473, 547, 824, 995, 1004, 1609, 2341, 2672
NciI	CCSGG	2 cut(s)	345, 2556
NdeI	CATATG	1 cut(s)	2158
NheI	GCTAGC	1 cut(s)	1534
NlaIV	GGNNCC	1 cut(s)	1813
NmeAIII	GCCGAG	2 cut(s)	1576, 2902
NmuCI	GTSAC	4 cut(s)	1125, 1186, 2485, 2815
NspI	RCATGY	2 cut(s)	1525, 2704
PaeR7I	CTCGAG	2 cut(s)	287, 993
PagI	TCATGA	2 cut(s)	66, 1089
PaqCI	CACCTGC	1 cut(s)	1075
PciI	ACATGT	1 cut(s)	1521
PciSI	GCTCTTC	1 cut(s)	1005
PcsI	WCGNNNNNNNCGW	2 cut(s)	156, 2874
PdmI	GAANNNNTTC	1 cut(s)	1920
PfeI	GAWTC	5 cut(s)	96, 1310, 1892, 1949, 2323
PflMI	CCANNNNNTGG	1 cut(s)	263
PfoI	TCCNGGA	1 cut(s)	1246
PleI	GAGTC	4 cut(s)	403, 1132, 1330, 2181
PpsI	GAGTC	4 cut(s)	403, 1132, 1330, 2181
PscI	ACATGT	1 cut(s)	1521
Psp124BI	GAGCTC	3 cut(s)	469, 1000, 1614
Psp1406I	AACGTT	2 cut(s)	2210, 2727
Psp6I	CCWGG	7 cut(s)	370, 509, 661, 1112, 1246, 2444, 2539
PspGI	CCWGG	7 cut(s)	370, 509, 661, 1112, 1246, 2444, 2539
PspN4I	GGNNCC	1 cut(s)	1813
PspPI	GGNCC	4 cut(s)	246, 1548, 1832, 2624
PspXI	VCTCGAGB	2 cut(s)	287, 993
PsrI	GAACNNNNNNTAC	4 cut(s)	1261, 1293, 1461, 1493
PstI	CTGCAG	1 cut(s)	555
PsuI	RGATCY	2 cut(s)	48, 1639
RsaI	GTAC	6 cut(s)	74, 149, 271, 1457, 1470, 2003
RsaNI	GTAC	6 cut(s)	73, 148, 270, 1456, 1469, 2002
RseI	CAYNNNNRTG	1 cut(s)	1761
SacI	GAGCTC	3 cut(s)	469, 1000, 1614
SapI	GCTCTTC	1 cut(s)	1005
SaqAI	TTAA	7 cut(s)	324, 569, 770, 1728, 2145, 2214, 2253
Sau96I	GGNCC	4 cut(s)	246, 1548, 1832, 2624
SchI	GAGTC	4 cut(s)	404, 1133, 1330, 2181
ScrFI	CCNGG	9 cut(s)	345, 372, 511, 663, 1114, 1248, 2446, 2541, 2556
SduI	GDGCHC	4 cut(s)	94, 469, 1000, 1614
SfcI	CTRYAG	3 cut(s)	551, 2348, 2475
Sfr274I	CTCGAG	2 cut(s)	287, 993
SinI	GGWCC	2 cut(s)	246, 1832
SlaI	CTCGAG	2 cut(s)	287, 993
SmiMI	CAYNNNNRTG	1 cut(s)	1761
SmlI	CTYRAG	6 cut(s)	53, 287, 734, 993, 2243, 2336
SmoI	CTYRAG	6 cut(s)	53, 287, 734, 993, 2243, 2336
SstI	GAGCTC	3 cut(s)	469, 1000, 1614
StyD4I	CCNGG	9 cut(s)	343, 370, 509, 661, 1112, 1246, 2444, 2539, 2554
StyI	CCWWGG	1 cut(s)	1025
TaaI	ACNGT	3 cut(s)	865, 2491, 2836
TaiI	ACGT	3 cut(s)	2213, 2730, 2817
TaqI	TCGA	8 cut(s)	94, 159, 288, 398, 994, 1304, 1592, 1992
TatI	WGTACW	1 cut(s)	2001
TauI	GCSGC	4 cut(s)	62, 370, 2285, 2398
TfiI	GAWTC	5 cut(s)	96, 1310, 1892, 1949, 2323
Tru1I	TTAA	7 cut(s)	324, 569, 770, 1728, 2145, 2214, 2253
Tru9I	TTAA	7 cut(s)	324, 569, 770, 1728, 2145, 2214, 2253
TscAI	CASTG	7 cut(s)	92, 137, 287, 702, 1828, 2233, 2839
TseFI	GTSAC	4 cut(s)	1125, 1186, 2485, 2815
Tsp45I	GTSAC	4 cut(s)	1125, 1186, 2485, 2815
TspGWI	ACGGA	4 cut(s)	53, 264, 373, 1813
TspRI	CASTG	7 cut(s)	92, 137, 287, 702, 1828, 2233, 2839
Van91I	CCANNNNNTGG	1 cut(s)	263
VpaK11BI	GGWCC	2 cut(s)	246, 1832
XagI	CCTNNNNNAGG	2 cut(s)	660, 1269
XceI	RCATGY	2 cut(s)	1525, 2704
XhoI	CTCGAG	2 cut(s)	287, 993
XmiI	GTMKAC	1 cut(s)	1791
XmnI	GAANNNNTTC	1 cut(s)	1920

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.