Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0003901

Physical Location & Seq

Genomic Coordinates Reverse (-)

26029715 .. 26031088

Gene Structure 1374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG229600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGAGCAACAAGATGATGATCCGAGGAGGCCTGTGGAAGAACACTGAGGATGAGATCCTGAAGGTGGCAGTCATGAAATATGGAATCAACGAGTGGGATCGCATCTCAACGCTGTTGAATCGCAAATCCGCAAAGCAGTGTAATGCTCGCTGGTTCCAGTGGCTCGAACCCTCAATCAAGAAGGTCGAGTGGACTCGAGAAGAGGACGAGAAGCTTCTCCATCTTGCAAAGCTCATGCCTTCACAGTGGAGAACAATAGCCAAAAACGTTAATGGTCGTACTGCATCTCAATGCATTGAGAGGTATGAGAAGCTTCTCGATGCAGCATGTAATGATAACTATGAAGATCCGAGGAGATTGAGGGCAGGAGAGATTGATCCCAACCCCGAGACCAGGCCGGCTCGCCCGGACCCTGTGGACATGGATGAGGATGAGAAGGAGATGCTTTCAGAGGCTCGAGCTCGGTTGGCTAACACCAAAGGGAAGAAGGCGAAGAGGAAGGCTAGAGAGGCACAGCTCGAAGAGGCTAGAAGGATTGCTTCTCTACAGAAGAGGAGAGAATTAAAGGCTGCAGGGATTGATGATAAGAGTACTTGGAATCAGCAGAAGAGGAAAAGGAATGGAATAGACTACAGTGCAGAAATTCCCTTTGAGAAGAACCCTCCTTTGGGGTTTTATAATGTCGGTGATGAGGACAGAGAAGTAGTATTGGAGCAGCATGATCAGTTTCCCTTGACTATTGAGGAGCTTGAAGGGAAGAGAAGGGTTGATGTGGAAGCTGAGCTGAGAAAACAAGATATTGCAAAGAACAAAATTTCCAACAAGTTGAATATTGATGATTTTTCCAATGTGGAAACAGTTAGGAAGAGATCAAAACTTATGCTTCCTGCACCGCAGATATCTGACATTGAATTGGAGGAGATTGCTAAGATGAGCAATGCAGGTGATCTTGTTGGCAGCGAGAATGGATTCACAGATGCAACTTATTTCGGGGTTACTCATGCAACTCGTAGATTAGGCTTAACACCATCAAAGGGTACTGTCATTAGGGATGAGCTCCATATCAATGAAGGCATTGATAATGATGATGGAGCAAAATTGAAGCAATTAGAGCATTCTCAGCAGTTCAGAATAAATAACTTGCATAAGGGTTTAAGCAATTTGCCAAAACCCAAAAGTGAGTACCAAATAGTTGTGCAACCAGTCCCAGAAGATAATGAAGAAGCGGGACATCGGGCATTAGTTACAAAGAAGAGATCAAAAGTACTGAATATGAGAGCAGATGAGGATAAGCATGGCCCGGTAGCTGTTCTTCCTAGTACAATTGAGCAGGCTGACCAAATGATCAGAAAGTTGTGCAAAGTATCCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009870 GO:0009889 GO:0009987 GO:0010204 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0030154 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032502 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Myb_Cef Myb_DNA-binding

Protein Analysis

457

Amino Acids

52.42

Weight (kDa)

8.59

Isoelectric Point (pI)

52.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	10 - 55	4.1e-11	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	12 - 72	2.1e-12	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	61 - 106	2.9e-13	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	64 - 111	3.2e-10	Myb-like DNA-binding domain
Myb_Cef	PF11831	332 - 416	2.2e-12	pre-mRNA splicing factor component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0003901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09770
fragaria_vesca	FvH4_3g19200 FvH4_3g19210 FvH4_6g01950
malus_domestica	MD04G1229500.v1.1 MD12G1246400.v1.1
prunus_persica	Prupe.6G349400_v2.0.a1
pyrus_communis	pycom04g20330 pycom04g20340 pycom12g22600
rosa_chinensis	RchiOBHm_Chr3g0449801 RchiOBHm_Chr5g0032281
rosa_laevigata	RLG00000025799 RLG00000033377
rosa_multiflora	Rmu_co8441867.1_g000001 Rmu_sc0000610.1_g000011 Rmu_sc0013983.1_g000008 Rmu_ssc0000013.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048170 Rroxscaffold_1G00048180 Rroxscaffold_6G00427620
rosa_rugosa	Rorug02G0623400 Rorug02G0623400 Rorug05G0130300
rosa_samantha	Rh3AG021600 Rh3BG022300 Rh3CG020900 Rh3DG022000 Rh5BG224900 Rh5CG250300 Rh5DG229500 Rh5DG229600
rosa_wichuraiana	Rw3G001770 Rw5G020450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	678
AarI	CACCTGC	1 cut(s)	932
Acc36I	ACCTGC	1 cut(s)	932
AciI	CCGC	3 cut(s)	129, 893, 1226
AclI	AACGTT	1 cut(s)	267
AclWI	GGATC	5 cut(s)	13, 49, 105, 341, 371
AcsI	RAATTY	2 cut(s)	642, 813
AcuI	CTGAAG	1 cut(s)	80
AfaI	GTAC	6 cut(s)	280, 592, 1039, 1184, 1266, 1321
AfiI	CCNNNNNNNGG	5 cut(s)	64, 393, 667, 668, 1034
AgsI	TTSAA	5 cut(s)	118, 752, 829, 911, 1102
AjnI	CCWGG	1 cut(s)	392
AjuI	GAANNNNNNNTTGG	2 cut(s)	650, 682
AloI	GAACNNNNNNTCC	2 cut(s)	800, 832
Alw21I	GWGCWC	2 cut(s)	463, 1059
Alw26I	GTCTC	1 cut(s)	383
AlwI	GGATC	5 cut(s)	13, 49, 105, 341, 371
Ama87I	CYCGRG	3 cut(s)	195, 386, 456
AoxI	GGCC	3 cut(s)	28, 395, 1297
ApeKI	GCWGC	4 cut(s)	323, 569, 715, 957
ApoI	RAATTY	2 cut(s)	642, 813
Asp700I	GAANNNNTTC	1 cut(s)	968
AspS9I	GGNCC	2 cut(s)	409, 1298
AsuC2I	CCSGG	2 cut(s)	407, 1301
AsuHPI	GGTGA	2 cut(s)	698, 956
AvaI	CYCGRG	3 cut(s)	195, 386, 456
AvaII	GGWCC	1 cut(s)	409
BanII	GRGCYC	2 cut(s)	463, 1059
BarI	GAAGNNNNNNTAC	2 cut(s)	1296, 1328
Bbv12I	GWGCWC	2 cut(s)	463, 1059
BbvI	GCAGC	4 cut(s)	335, 556, 727, 969
BccI	CCATC	3 cut(s)	228, 1036, 1082
BciT130I	CCWGG	1 cut(s)	394
BclI	TGATCA	2 cut(s)	721, 1344
BcnI	CCSGG	2 cut(s)	407, 1301
BcoDI	GTCTC	1 cut(s)	383
BfaI	CTAG	3 cut(s)	504, 528, 1317
BfmI	CTRYAG	3 cut(s)	545, 570, 631
BfuAI	ACCTGC	1 cut(s)	932
BisI	GCNGC	4 cut(s)	324, 570, 716, 958
BlpI	GCTNAGC	1 cut(s)	780
BlsI	GCNGC	4 cut(s)	325, 571, 717, 959
BmcAI	AGTACT	2 cut(s)	592, 1266
Bme1390I	CCNGG	3 cut(s)	394, 407, 1301
Bme18I	GGWCC	1 cut(s)	409
BmeT110I	CYCGRG	3 cut(s)	195, 386, 456
BmgT120I	GGNCC	2 cut(s)	409, 1298
BmiI	GGNNCC	2 cut(s)	155, 411
BmrFI	CCNGG	3 cut(s)	394, 407, 1301
BmsI	GCATC	5 cut(s)	111, 293, 310, 432, 967
Bpu1102I	GCTNAGC	1 cut(s)	780
BpuMI	CCSGG	2 cut(s)	407, 1301
BsaBI	GATNNNNATC	1 cut(s)	17
BsaI	GGTCTC	1 cut(s)	383
BsaJI	CCNNGG	2 cut(s)	22, 350
Bsc4I	CCNNNNNNNGG	5 cut(s)	64, 393, 667, 668, 1034
Bse118I	RCCGGY	1 cut(s)	397
Bse1I	ACTGG	2 cut(s)	157, 1202
Bse3DI	GCAATG	1 cut(s)	943
Bse8I	GATNNNNATC	1 cut(s)	17
BseBI	CCWGG	1 cut(s)	394
BseDI	CCNNGG	2 cut(s)	22, 350
BseGI	GGATG	4 cut(s)	55, 430, 436, 1057
BseJI	GATNNNNATC	1 cut(s)	17
BseLI	CCNNNNNNNGG	5 cut(s)	64, 393, 667, 668, 1034
BseMI	GCAATG	1 cut(s)	943
BseMII	CTCAG	4 cut(s)	36, 771, 776, 1133
BseNI	ACTGG	2 cut(s)	157, 1202
BseRI	GAGGAG	5 cut(s)	39, 367, 568, 758, 932
BseXI	GCAGC	4 cut(s)	335, 556, 727, 969
BsgI	GTGCAG	2 cut(s)	657, 873
BshFI	GGCC	3 cut(s)	30, 397, 1299
BsiHKAI	GWGCWC	2 cut(s)	463, 1059
BsiHKCI	CYCGRG	3 cut(s)	195, 386, 456
BsiSI	CCGG	3 cut(s)	398, 407, 1301
BslFI	GGGAC	2 cut(s)	1190, 1242
BslI	CCNNNNNNNGG	5 cut(s)	64, 393, 667, 668, 1034
BsmAI	GTCTC	1 cut(s)	383
BsmFI	GGGAC	2 cut(s)	1190, 1242
BsmI	GAATGC	1 cut(s)	1114
BsnI	GGCC	3 cut(s)	30, 397, 1299
Bso31I	GGTCTC	1 cut(s)	383
BsoBI	CYCGRG	3 cut(s)	195, 386, 456
Bsp1286I	GDGCHC	2 cut(s)	463, 1059
Bsp1720I	GCTNAGC	1 cut(s)	780
BspACI	CCGC	3 cut(s)	129, 893, 1226
BspANI	GGCC	3 cut(s)	30, 397, 1299
BspCNI	CTCAG	4 cut(s)	37, 772, 777, 1132
BspHI	TCATGA	1 cut(s)	72
BspLI	GGNNCC	2 cut(s)	155, 411
BspMAI	CTGCAG	1 cut(s)	574
BspMI	ACCTGC	1 cut(s)	932
BspPI	GGATC	5 cut(s)	13, 49, 105, 341, 371
BspTNI	GGTCTC	1 cut(s)	383
BsrDI	GCAATG	1 cut(s)	943
BsrFI	RCCGGY	1 cut(s)	397
BsrI	ACTGG	2 cut(s)	157, 1202
BssAI	RCCGGY	1 cut(s)	397
BssECI	CCNNGG	2 cut(s)	22, 350
Bst2UI	CCWGG	1 cut(s)	394
Bst4CI	ACNGT	4 cut(s)	246, 635, 859, 1042
Bst6I	CTCTTC	8 cut(s)	195, 488, 516, 545, 602, 752, 860, 1247
BstC8I	GCNNGC	4 cut(s)	148, 399, 403, 1332
BstDEI	CTNAG	5 cut(s)	45, 780, 785, 927, 1119
BstF5I	GGATG	4 cut(s)	55, 430, 436, 1057
BstMAI	GTCTC	1 cut(s)	383
BstMWI	GCNNNNNNNGC	4 cut(s)	467, 509, 1111, 1120
BstNI	CCWGG	1 cut(s)	394
BstNSI	RCATGY	1 cut(s)	330
BstSCI	CCNGG	3 cut(s)	392, 405, 1299
BstSFI	CTRYAG	3 cut(s)	545, 570, 631
BstV1I	GCAGC	4 cut(s)	335, 556, 727, 969
BstX2I	RGATCY	2 cut(s)	54, 346
BstYI	RGATCY	2 cut(s)	54, 346
BsuRI	GGCC	3 cut(s)	30, 397, 1299
BtsCI	GGATG	4 cut(s)	55, 430, 436, 1057
BtsI	GCAGTG	1 cut(s)	143
BtsIMutI	CAGTG	5 cut(s)	42, 143, 164, 251, 640
BveI	ACCTGC	1 cut(s)	932
Cac8I	GCNNGC	4 cut(s)	148, 399, 403, 1332
CciI	TCATGA	1 cut(s)	72
Cfr10I	RCCGGY	1 cut(s)	397
Cfr13I	GGNCC	2 cut(s)	409, 1298
Csp6I	GTAC	6 cut(s)	279, 591, 1038, 1183, 1265, 1320
CviAII	CATG	7 cut(s)	73, 235, 327, 421, 719, 1001, 1295
CviQI	GTAC	6 cut(s)	279, 591, 1038, 1183, 1265, 1320
DdeI	CTNAG	5 cut(s)	45, 780, 785, 927, 1119
Eam1104I	CTCTTC	8 cut(s)	195, 488, 516, 545, 602, 752, 860, 1247
EarI	CTCTTC	8 cut(s)	195, 488, 516, 545, 602, 752, 860, 1247
Ecl136II	GAGCTC	2 cut(s)	461, 1057
Eco147I	AGGCCT	1 cut(s)	30
Eco24I	GRGCYC	2 cut(s)	463, 1059
Eco31I	GGTCTC	1 cut(s)	383
Eco32I	GATATC	1 cut(s)	900
Eco47I	GGWCC	1 cut(s)	409
Eco53kI	GAGCTC	2 cut(s)	461, 1057
Eco57I	CTGAAG	1 cut(s)	80
Eco88I	CYCGRG	3 cut(s)	195, 386, 456
EcoICRI	GAGCTC	2 cut(s)	461, 1057
EcoRII	CCWGG	1 cut(s)	392
EcoRV	GATATC	1 cut(s)	900
EcoT22I	ATGCAT	1 cut(s)	296
EcoT38I	GRGCYC	2 cut(s)	463, 1059
FaeI	CATG	7 cut(s)	76, 238, 330, 424, 722, 1004, 1298
FalI	AAGNNNNNCTT	2 cut(s)	523, 555
FaqI	GGGAC	2 cut(s)	1190, 1242
FatI	CATG	7 cut(s)	72, 234, 326, 420, 718, 1000, 1294
FauI	CCCGC	1 cut(s)	1219
FbaI	TGATCA	2 cut(s)	721, 1344
Fnu4HI	GCNGC	4 cut(s)	324, 570, 716, 958
FokI	GGATG	4 cut(s)	62, 437, 443, 1064
FriOI	GRGCYC	2 cut(s)	463, 1059
Fsp4HI	GCNGC	4 cut(s)	324, 570, 716, 958
FspBI	CTAG	3 cut(s)	504, 528, 1317
GluI	GCNGC	4 cut(s)	324, 570, 716, 958
HaeIII	GGCC	3 cut(s)	30, 397, 1299
HapII	CCGG	3 cut(s)	398, 407, 1301
Hin1II	CATG	7 cut(s)	76, 238, 330, 424, 722, 1004, 1298
HindIII	AAGCTT	2 cut(s)	212, 311
HinfI	GANTC	5 cut(s)	84, 118, 193, 598, 969
HpaII	CCGG	3 cut(s)	398, 407, 1301
HphI	GGTGA	2 cut(s)	698, 956
Hpy166II	GTNNAC	2 cut(s)	192, 418
Hpy188I	TCNGA	6 cut(s)	23, 351, 451, 904, 1130, 1349
Hpy188III	TCNNGA	5 cut(s)	58, 73, 178, 197, 317
Hpy8I	GTNNAC	2 cut(s)	192, 418
HpyCH4III	ACNGT	4 cut(s)	246, 635, 859, 1042
HpyCH4IV	ACGT	1 cut(s)	267
HpyF10VI	GCNNNNNNNGC	4 cut(s)	467, 509, 1111, 1120
HpyF3I	CTNAG	5 cut(s)	45, 780, 785, 927, 1119
HpySE526I	ACGT	1 cut(s)	267
Hsp92II	CATG	7 cut(s)	76, 238, 330, 424, 722, 1004, 1298
KroI	GCCGGC	1 cut(s)	397
KroNI	GCCGGC	1 cut(s)	399
Ksp22I	TGATCA	2 cut(s)	721, 1344
LmnI	GCTCC	4 cut(s)	712, 745, 1062, 1091
Lsp1109I	GCAGC	4 cut(s)	335, 556, 727, 969
LweI	GCATC	5 cut(s)	111, 293, 310, 432, 967
MaeI	CTAG	3 cut(s)	504, 528, 1317
MaeII	ACGT	1 cut(s)	267
MaeIII	GTNAC	2 cut(s)	994, 1243
MfeI	CAATTG	2 cut(s)	1323, 1369
MflI	RGATCY	2 cut(s)	54, 346
MhlI	GDGCHC	2 cut(s)	463, 1059
MluCI	AATT	9 cut(s)	560, 642, 813, 911, 1097, 1106, 1159, 1323, 1369
MlyI	GAGTC	1 cut(s)	187
MmeI	TCCRAC	1 cut(s)	843
Mph1103I	ATGCAT	1 cut(s)	296
MroNI	GCCGGC	1 cut(s)	397
MroXI	GAANNNNTTC	1 cut(s)	968
MseI	TTAA	4 cut(s)	270, 563, 1022, 1154
MslI	CAYNNNNRTG	2 cut(s)	289, 1065
MspI	CCGG	3 cut(s)	398, 407, 1301
MspR9I	CCNGG	3 cut(s)	394, 407, 1301
MunI	CAATTG	2 cut(s)	1323, 1369
Mva1269I	GAATGC	1 cut(s)	1114
MvaI	CCWGG	1 cut(s)	394
MwoI	GCNNNNNNNGC	4 cut(s)	467, 509, 1111, 1120
NaeI	GCCGGC	1 cut(s)	399
NciI	CCSGG	2 cut(s)	407, 1301
NgoMIV	GCCGGC	1 cut(s)	397
NlaIII	CATG	7 cut(s)	76, 238, 330, 424, 722, 1004, 1298
NlaIV	GGNNCC	2 cut(s)	155, 411
NsiI	ATGCAT	1 cut(s)	296
NspI	RCATGY	1 cut(s)	330
PaeR7I	CTCGAG	2 cut(s)	195, 456
PagI	TCATGA	1 cut(s)	72
PaqCI	CACCTGC	1 cut(s)	932
PceI	AGGCCT	1 cut(s)	30
PctI	GAATGC	1 cut(s)	1114
PdiI	GCCGGC	1 cut(s)	399
PdmI	GAANNNNTTC	1 cut(s)	968
PfeI	GAWTC	4 cut(s)	84, 118, 598, 969
PkrI	GCNGC	4 cut(s)	325, 571, 717, 959
PleI	GAGTC	1 cut(s)	187
PpsI	GAGTC	1 cut(s)	187
PsiI	TTATAA	1 cut(s)	678
Psp124BI	GAGCTC	2 cut(s)	463, 1059
Psp1406I	AACGTT	1 cut(s)	267
Psp6I	CCWGG	1 cut(s)	392
PspGI	CCWGG	1 cut(s)	392
PspN4I	GGNNCC	2 cut(s)	155, 411
PspPI	GGNCC	2 cut(s)	409, 1298
PspXI	VCTCGAGB	1 cut(s)	456
PstI	CTGCAG	1 cut(s)	574
PsuI	RGATCY	2 cut(s)	54, 346
RsaI	GTAC	6 cut(s)	280, 592, 1039, 1184, 1266, 1321
RsaNI	GTAC	6 cut(s)	279, 591, 1038, 1183, 1265, 1320
RseI	CAYNNNNRTG	2 cut(s)	289, 1065
SacI	GAGCTC	2 cut(s)	463, 1059
SaqAI	TTAA	4 cut(s)	270, 563, 1022, 1154
SatI	GCNGC	4 cut(s)	324, 570, 716, 958
Sau96I	GGNCC	2 cut(s)	409, 1298
ScaI	AGTACT	2 cut(s)	592, 1266
SchI	GAGTC	1 cut(s)	187
ScrFI	CCNGG	3 cut(s)	394, 407, 1301
SduI	GDGCHC	2 cut(s)	463, 1059
SfaNI	GCATC	5 cut(s)	111, 293, 310, 432, 967
SfcI	CTRYAG	3 cut(s)	545, 570, 631
Sfr274I	CTCGAG	2 cut(s)	195, 456
SinI	GGWCC	1 cut(s)	409
SlaI	CTCGAG	2 cut(s)	195, 456
SmiMI	CAYNNNNRTG	2 cut(s)	289, 1065
SmlI	CTYRAG	2 cut(s)	195, 456
SmoI	CTYRAG	2 cut(s)	195, 456
Sse9I	AATT	9 cut(s)	560, 642, 813, 911, 1097, 1106, 1159, 1323, 1369
SseBI	AGGCCT	1 cut(s)	30
SsiI	CCGC	3 cut(s)	129, 893, 1226
SspI	AATATT	1 cut(s)	832
SspMI	CTAG	3 cut(s)	504, 528, 1317
SstI	GAGCTC	2 cut(s)	463, 1059
StuI	AGGCCT	1 cut(s)	30
StyD4I	CCNGG	3 cut(s)	392, 405, 1299
TaaI	ACNGT	4 cut(s)	246, 635, 859, 1042
TaiI	ACGT	1 cut(s)	270
TaqI	TCGA	6 cut(s)	165, 186, 196, 318, 457, 519
TasI	AATT	9 cut(s)	560, 642, 813, 911, 1097, 1106, 1159, 1323, 1369
TatI	WGTACW	3 cut(s)	590, 1264, 1319
TfiI	GAWTC	4 cut(s)	84, 118, 598, 969
Tru1I	TTAA	4 cut(s)	270, 563, 1022, 1154
Tru9I	TTAA	4 cut(s)	270, 563, 1022, 1154
TscAI	CASTG	5 cut(s)	49, 143, 164, 251, 640
TseI	GCWGC	4 cut(s)	323, 569, 715, 957
TspDTI	ATGAA	4 cut(s)	89, 357, 1083, 1233
TspRI	CASTG	5 cut(s)	49, 143, 164, 251, 640
VpaK11BI	GGWCC	1 cut(s)	409
XapI	RAATTY	2 cut(s)	642, 813
XceI	RCATGY	1 cut(s)	330
XhoI	CTCGAG	2 cut(s)	195, 456
XmnI	GAANNNNTTC	1 cut(s)	968
XspI	CTAG	3 cut(s)	504, 528, 1317
ZrmI	AGTACT	2 cut(s)	592, 1266
Zsp2I	ATGCAT	1 cut(s)	296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.