MD05G1035800.v1.1

MRN-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009611
Physical Location & Seq
Forward (+)
5754268 .. 5755702
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1035800.v1.1.491

Sequence Viewer

Length: 825 bp
ATGATTTTGTTTGGCGATGAATGCCGATTAAATTTGCAGGTGAAGCAGAGGAACAAAAGCAACAGGTGGACATGCGTCGTCTGCAATCAGAAGCAGTCCGTCCGCCAAGTGTTTGCTCAAGGTCCCATGGCCAGAGACCTCCGCCTCTTCGTCCAGTCCTCCAACATGTCTCGCCAGTTCGCCCAGCAAACGCATGATCAACAGCAACACCAACAGCGACAAGAACAAGATACTCTTATTTCTTCCGATTGCTCGTATAAGACCGAAAAGCGAAGACGAAATGACTGGACCCAGTACCTGGATCCTGAGGACAGTGATCGGGGCGCCAACATGAAGCCAGAAGACGAAGCAGGTTCTGGTTTTGAGCCTGAGATTGTAACAGAGTTTCCGGCGGAGTTGTGGAAGAAACCAAAGCTGAGCAAATATGGTGTAGATGAGCTGCACAGTCACAAACCGATTTTCTCTAAGAGGAATTTCAACAAACATGTCATCTCCCTTCCACAAGACACAGAACAGGCAAGGCGCGAGCTGACAGACACCAGGGAGAGTTCACAAGTACAGAGGCATTTTATAGCAAGAGATGAGCAGGAACCGAGAAGAACATGTCAGGCTACAAAGGCTACACTAACTGGTGCTTCTAAATGGAGTGACTATGTAACCCAAGACGAGGAAGACGATTGCAGCTTGCGACTCATTACAAGGAGAAAACATGCAGACAATGCAGGCCAATGCAGCAATGAAATTGACAAGACAACAGCAAACTATGAGACAGTTCTGGCAAATGATGAGACGGTAGAAGATGACATTCATCCTGATTTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRNIP

Protein Analysis

275

Amino Acids

31.97

Weight (kDa)

5.82

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRNIP PF15749 8 - 99 2.8e-16 MRN-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 2 cut(s) 28, 341
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 298
AccII CGCG 1 cut(s) 525
AciI CCGC 3 cut(s) 103, 142, 392
AclWI GGATC 2 cut(s) 296, 309
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 31, 472
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 2 cut(s) 296, 558
AfiI CCNNNNNNNGG 2 cut(s) 298, 667
AflIII ACRYGT 3 cut(s) 165, 484, 602
AgsI TTSAA 1 cut(s) 478
AjnI CCWGG 2 cut(s) 297, 539
AluBI AGCT 4 cut(s) 415, 439, 529, 684
AluI AGCT 4 cut(s) 415, 439, 529, 684
Alw26I GTCTC 4 cut(s) 129, 174, 761, 782
AlwI GGATC 2 cut(s) 296, 309
AlwNI CAGNNNCTG 2 cut(s) 298, 356
AoxI GGCC 2 cut(s) 129, 724
ApeKI GCWGC 3 cut(s) 439, 681, 732
ApoI RAATTY 2 cut(s) 31, 472
AspLEI GCGC 2 cut(s) 326, 525
AspS9I GGNCC 2 cut(s) 122, 288
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 2 cut(s) 122, 288
AxyI CCTNAGG 1 cut(s) 306
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 323
BbsI GAAGAC 3 cut(s) 280, 348, 678
BbvI GCAGC 3 cut(s) 426, 693, 744
BciT130I CCWGG 2 cut(s) 299, 541
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 4 cut(s) 129, 174, 761, 782
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 2 cut(s) 28, 341
BisI GCNGC 3 cut(s) 440, 682, 733
BlpI GCTNAGC 1 cut(s) 416
BlsI GCNGC 3 cut(s) 441, 683, 734
Bme1390I CCNGG 2 cut(s) 299, 541
Bme18I GGWCC 2 cut(s) 122, 288
BmgT120I GGNCC 2 cut(s) 122, 288
BmiI GGNNCC 5 cut(s) 124, 290, 303, 325, 591
BmrFI CCNGG 2 cut(s) 299, 541
BmrI ACTGGG 1 cut(s) 286
BmuI ACTGGG 1 cut(s) 286
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 3 cut(s) 280, 348, 678
Bpu1102I GCTNAGC 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 126, 540
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 667
Bse1I ACTGG 5 cut(s) 154, 175, 290, 292, 634
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 742
BseBI CCWGG 2 cut(s) 299, 541
BseDI CCNNGG 2 cut(s) 126, 540
BseGI GGATG 1 cut(s) 808
BseLI CCNNNNNNNGG 2 cut(s) 298, 667
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 3 cut(s) 297, 360, 407
BseNI ACTGG 5 cut(s) 154, 175, 290, 292, 634
BseXI GCAGC 3 cut(s) 426, 693, 744
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 425
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 131, 726
BshNI GGYRCC 1 cut(s) 323
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 2 cut(s) 298, 667
BsmAI GTCTC 4 cut(s) 129, 174, 761, 782
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 131, 726
Bso31I GGTCTC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 196, 301, 316
Bsp1720I GCTNAGC 1 cut(s) 416
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 3 cut(s) 103, 142, 392
BspANI GGCC 2 cut(s) 131, 726
BspCNI CTCAG 3 cut(s) 298, 361, 408
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 5 cut(s) 124, 290, 303, 325, 591
BspMI ACCTGC 2 cut(s) 28, 341
BspPI GGATC 2 cut(s) 296, 309
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 742
BsrI ACTGG 5 cut(s) 154, 175, 290, 292, 634
BssECI CCNNGG 2 cut(s) 126, 540
BssMI GATC 3 cut(s) 196, 301, 316
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 299, 541
Bst4CI ACNGT 4 cut(s) 314, 446, 772, 793
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 719
BstC8I GCNNGC 3 cut(s) 527, 686, 724
BstDEI CTNAG 4 cut(s) 306, 369, 416, 465
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 808
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 2 cut(s) 326, 525
BstKTI GATC 3 cut(s) 199, 304, 319
BstMAI GTCTC 4 cut(s) 129, 174, 761, 782
BstMBI GATC 3 cut(s) 196, 301, 316
BstMWI GCNNNNNNNGC 6 cut(s) 21, 43, 81, 617, 719, 732
BstNI CCWGG 2 cut(s) 299, 541
BstNSI RCATGY 5 cut(s) 75, 169, 488, 606, 713
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 297, 539
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 3 cut(s) 426, 693, 744
BstV2I GAAGAC 3 cut(s) 280, 348, 678
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 2 cut(s) 131, 726
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 808
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 2 cut(s) 28, 341
Cac8I GCNNGC 3 cut(s) 527, 686, 724
CaiI CAGNNNCTG 2 cut(s) 298, 356
CfoI GCGC 2 cut(s) 326, 525
Cfr13I GGNCC 2 cut(s) 122, 288
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 295, 557
CviAII CATG 8 cut(s) 72, 127, 166, 194, 331, 485, 603, 710
CviQI GTAC 2 cut(s) 295, 557
DdeI CTNAG 4 cut(s) 306, 369, 416, 465
DinI GGCGCC 1 cut(s) 325
DpnI GATC 3 cut(s) 198, 303, 318
DpnII GATC 3 cut(s) 196, 301, 316
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 3 cut(s) 92, 131, 407
Eco130I CCWWGG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 122, 288
Eco81I CCTNAGG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 297, 539
EcoT14I CCWWGG 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 8 cut(s) 75, 130, 169, 197, 334, 488, 606, 713
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 1 cut(s) 108
FatI CATG 8 cut(s) 71, 126, 165, 193, 330, 484, 602, 709
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 3 cut(s) 440, 682, 733
FokI GGATG 1 cut(s) 795
Fsp4HI GCNGC 3 cut(s) 440, 682, 733
GlaI GCGC 2 cut(s) 325, 524
GluI GCNGC 3 cut(s) 440, 682, 733
GsaI CCCAGC 1 cut(s) 187
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 131, 726
HapII CCGG 1 cut(s) 389
HgaI GACGC 1 cut(s) 64
HhaI GCGC 2 cut(s) 326, 525
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 8 cut(s) 75, 130, 169, 197, 334, 488, 606, 713
Hin6I GCGC 2 cut(s) 324, 523
HinP1I GCGC 2 cut(s) 324, 523
HinfI GANTC 1 cut(s) 690
HpaII CCGG 1 cut(s) 389
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 2 cut(s) 69, 551
Hpy188I TCNGA 2 cut(s) 90, 247
Hpy188III TCNNGA 2 cut(s) 305, 812
Hpy8I GTNNAC 2 cut(s) 69, 551
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 4 cut(s) 314, 446, 772, 793
HpyCH4V TGCA 7 cut(s) 37, 84, 442, 681, 713, 722, 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 43, 81, 617, 719, 732
HpyF3I CTNAG 4 cut(s) 306, 369, 416, 465
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 8 cut(s) 75, 130, 169, 197, 334, 488, 606, 713
HspAI GCGC 2 cut(s) 324, 523
KasI GGCGCC 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 3 cut(s) 196, 301, 316
Lsp1109I GCAGC 3 cut(s) 426, 693, 744
MaeIII GTNAC 4 cut(s) 376, 446, 647, 655
MalI GATC 3 cut(s) 198, 303, 318
MboI GATC 3 cut(s) 196, 301, 316
MboII GAAGA 8 cut(s) 139, 234, 285, 353, 415, 609, 683, 809
MflI RGATCY 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 3 cut(s) 31, 472, 741
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 684
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 8 cut(s) 42, 149, 155, 169, 301, 462, 555, 661
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 131
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 299, 541
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 2 cut(s) 299, 541
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 6 cut(s) 21, 43, 81, 617, 719, 732
NarI GGCGCC 1 cut(s) 324
NcoI CCATGG 1 cut(s) 126
NdeII GATC 3 cut(s) 196, 301, 316
NlaIII CATG 8 cut(s) 75, 130, 169, 197, 334, 488, 606, 713
NlaIV GGNNCC 5 cut(s) 124, 290, 303, 325, 591
NmuCI GTSAC 2 cut(s) 446, 647
NspI RCATGY 5 cut(s) 75, 169, 488, 606, 713
PaqCI CACCTGC 1 cut(s) 28
PciI ACATGT 3 cut(s) 165, 484, 602
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 298
PkrI GCNGC 3 cut(s) 441, 683, 734
PleI GAGTC 1 cut(s) 684
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 684
PpuMI RGGWCCY 1 cut(s) 122
PscI ACATGT 3 cut(s) 165, 484, 602
PshAI GACNNNNGTC 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 297, 539
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 2 cut(s) 297, 539
PspN4I GGNNCC 5 cut(s) 124, 290, 303, 325, 591
PspPI GGNCC 2 cut(s) 122, 288
PspPPI RGGWCCY 1 cut(s) 122
PstNI CAGNNNCTG 2 cut(s) 298, 356
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 296, 558
RsaNI GTAC 2 cut(s) 295, 557
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 3 cut(s) 440, 682, 733
Sau3AI GATC 3 cut(s) 196, 301, 316
Sau96I GGNCC 2 cut(s) 122, 288
SchI GAGTC 1 cut(s) 684
ScrFI CCNGG 2 cut(s) 299, 541
SfoI GGCGCC 1 cut(s) 325
SinI GGWCC 2 cut(s) 122, 288
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
Sse9I AATT 3 cut(s) 31, 472, 741
SsiI CCGC 3 cut(s) 103, 142, 392
SspDI GGCGCC 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 297, 539
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 4 cut(s) 314, 446, 772, 793
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 31, 472, 741
TatI WGTACW 1 cut(s) 556
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 2 cut(s) 446, 647
TseI GCWGC 3 cut(s) 439, 681, 732
Tsp45I GTSAC 2 cut(s) 446, 647
TspDTI ATGAA 4 cut(s) 33, 347, 753, 797
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 298
VpaK11BI GGWCC 2 cut(s) 122, 288
XapI RAATTY 2 cut(s) 31, 472
XceI RCATGY 5 cut(s) 75, 169, 488, 606, 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.