pycom05g02630

MRN-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009611
Physical Location & Seq
Forward (+)
3114539 .. 3115719
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02630.2

Sequence Viewer

Length: 837 bp
ATGCTTTTGTTTGGCAATGAATGCCGATTAAATTTGCAGGTGAAGCAGAGGAACAAAAGCAACAAGTGGACATGCGCCGTCTGCAATCAGAAGCAGTCCGTCCGCCAAGTGTTTGCTCAAGGTCCCATGGCCAAAGACCTCCGCCTCTTCGTCCAGTCCTCCAACATGTCTCGCCAGTTCGCCCAGCAAACCAATGATCAACAGCAACAGCAACAGCAACAGCAACAGCGGCAAGAACAAGATACTCTTATTTCTTCCGATTGCTCGTACAAGACTGAAAAGAGAAGACGAAATGACTGGACCCAGTACCTGGACCCTGAGGACAGTGATCGGGGCGCCAACGTGAAGCCAGAAGACGAAGCAGGTTCTGGTTTTGAGCCTGAGATTGTAACAGAGTTTCCGGCGGAGTTGTGGAAGAAACCAAAGCTGAGCAAATATGGTGTAGATGAGCTGCACAGTCGCAAACCAGTTTTCTCTAAGAGGAATTCCAACAAACATGTCATCTCCCTTCCACAAGACACAGAGCAGGTGAAGTGCGAGCTGACAAACACCGAGGAGAGTTCACAAGTACAGAGGCATTTTATAGCAAGAGATGAGCAGGAACCGAGAAGAACATGTCAGGCTACAAAGGCTACACTAACTGGTGCTTCTAAATGGAGTGACTATGTAACCCAAGACGAGGATGACGATTGCAGCTTGCGACTCATTACAAGGAGAAAACATGCAGACAATGCAGGCCAATGCAGCAATGAGATTGACAAGACAACAACAAACTATGAGACAGTTCGGGCAAACGATGAGACGGTAGAAGATGACATTCATCCTGATTTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRNIP

Protein Analysis

279

Amino Acids

32.26

Weight (kDa)

5.69

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 28, 517
Acc36I ACCTGC 3 cut(s) 28, 353, 517
AccB1I GGYRCC 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 310
AciI CCGC 4 cut(s) 103, 142, 229, 404
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 31, 484
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 3 cut(s) 269, 308, 570
AfiI CCNNNNNNNGG 2 cut(s) 310, 679
AflIII ACRYGT 3 cut(s) 165, 496, 614
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 4 cut(s) 427, 451, 541, 696
AluI AGCT 4 cut(s) 427, 451, 541, 696
Alw26I GTCTC 3 cut(s) 174, 773, 794
AlwNI CAGNNNCTG 2 cut(s) 310, 368
AoxI GGCC 2 cut(s) 129, 736
ApeKI GCWGC 3 cut(s) 451, 693, 744
ApoI RAATTY 2 cut(s) 31, 484
AspLEI GCGC 2 cut(s) 77, 338
AspS9I GGNCC 3 cut(s) 122, 300, 313
AsuHPI GGTGA 2 cut(s) 52, 541
AvaII GGWCC 3 cut(s) 122, 300, 313
AxyI CCTNAGG 1 cut(s) 318
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 292, 360
BbvI GCAGC 3 cut(s) 438, 705, 756
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 311
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 3 cut(s) 174, 773, 794
BfoI RGCGCY 1 cut(s) 339
BfuAI ACCTGC 3 cut(s) 28, 353, 517
BisI GCNGC 4 cut(s) 230, 452, 694, 745
BlpI GCTNAGC 1 cut(s) 428
BlsI GCNGC 4 cut(s) 231, 453, 695, 746
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 3 cut(s) 122, 300, 313
BmgT120I GGNCC 3 cut(s) 122, 300, 313
BmiI GGNNCC 5 cut(s) 124, 302, 315, 337, 603
BmrFI CCNGG 1 cut(s) 311
BmrI ACTGGG 1 cut(s) 298
BmuI ACTGGG 1 cut(s) 298
BpiI GAAGAC 2 cut(s) 292, 360
Bpu1102I GCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 126, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 679
Bse1I ACTGG 6 cut(s) 154, 175, 302, 304, 467, 646
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 22, 754
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 126, 552
BseGI GGATG 2 cut(s) 688, 820
BseLI CCNNNNNNNGG 2 cut(s) 310, 679
BseMI GCAATG 2 cut(s) 22, 754
BseMII CTCAG 3 cut(s) 309, 372, 419
BseNI ACTGG 6 cut(s) 154, 175, 302, 304, 467, 646
BseRI GAGGAG 1 cut(s) 569
BseXI GCAGC 3 cut(s) 438, 705, 756
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 437
BshFI GGCC 2 cut(s) 131, 738
BshNI GGYRCC 1 cut(s) 335
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 2 cut(s) 310, 679
BsmAI GTCTC 3 cut(s) 174, 773, 794
BsmBI CGTCTC 1 cut(s) 794
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 131, 738
Bsp143I GATC 2 cut(s) 196, 328
Bsp1720I GCTNAGC 1 cut(s) 428
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 4 cut(s) 103, 142, 229, 404
BspANI GGCC 2 cut(s) 131, 738
BspCNI CTCAG 3 cut(s) 310, 373, 420
BspLI GGNNCC 5 cut(s) 124, 302, 315, 337, 603
BspMI ACCTGC 3 cut(s) 28, 353, 517
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 22, 754
BsrI ACTGG 6 cut(s) 154, 175, 302, 304, 467, 646
BssECI CCNNGG 2 cut(s) 126, 552
BssMI GATC 2 cut(s) 196, 328
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 4 cut(s) 326, 458, 784, 805
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 336
BstAPI GCANNNNNTGC 2 cut(s) 21, 731
BstC8I GCNNGC 3 cut(s) 539, 698, 736
BstDEI CTNAG 4 cut(s) 318, 381, 428, 477
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 688, 820
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 2 cut(s) 77, 338
BstKTI GATC 2 cut(s) 199, 331
BstMAI GTCTC 3 cut(s) 174, 773, 794
BstMBI GATC 2 cut(s) 196, 328
BstMWI GCNNNNNNNGC 7 cut(s) 21, 43, 81, 229, 629, 731, 744
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 5 cut(s) 75, 169, 500, 618, 725
BstSCI CCNGG 1 cut(s) 309
BstV1I GCAGC 3 cut(s) 438, 705, 756
BstV2I GAAGAC 2 cut(s) 292, 360
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 131, 738
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 688, 820
BtsIMutI CAGTG 1 cut(s) 331
BveI ACCTGC 3 cut(s) 28, 353, 517
Cac8I GCNNGC 3 cut(s) 539, 698, 736
CaiI CAGNNNCTG 2 cut(s) 310, 368
CfoI GCGC 2 cut(s) 77, 338
Cfr13I GGNCC 3 cut(s) 122, 300, 313
Csp6I GTAC 3 cut(s) 268, 307, 569
CviAII CATG 6 cut(s) 72, 127, 166, 497, 615, 722
CviQI GTAC 3 cut(s) 268, 307, 569
DdeI CTNAG 4 cut(s) 318, 381, 428, 477
DinI GGCGCC 1 cut(s) 337
DpnI GATC 2 cut(s) 198, 330
DpnII GATC 2 cut(s) 196, 328
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 3 cut(s) 92, 131, 419
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 3 cut(s) 122, 300, 313
Eco81I CCTNAGG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 122
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 6 cut(s) 75, 130, 169, 500, 618, 725
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 1 cut(s) 108
FatI CATG 6 cut(s) 71, 126, 165, 496, 614, 721
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 4 cut(s) 230, 452, 694, 745
FokI GGATG 2 cut(s) 695, 807
Fsp4HI GCNGC 4 cut(s) 230, 452, 694, 745
GlaI GCGC 2 cut(s) 76, 337
GluI GCNGC 4 cut(s) 230, 452, 694, 745
GsaI CCCAGC 1 cut(s) 187
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 2 cut(s) 131, 738
HapII CCGG 1 cut(s) 401
HhaI GCGC 2 cut(s) 77, 338
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 6 cut(s) 75, 130, 169, 500, 618, 725
Hin6I GCGC 2 cut(s) 75, 336
HinP1I GCGC 2 cut(s) 75, 336
HinfI GANTC 1 cut(s) 702
HpaII CCGG 1 cut(s) 401
HphI GGTGA 2 cut(s) 52, 541
Hpy166II GTNNAC 2 cut(s) 69, 563
Hpy188I TCNGA 2 cut(s) 90, 259
Hpy188III TCNNGA 1 cut(s) 824
Hpy8I GTNNAC 2 cut(s) 69, 563
HpyAV CCTTC 1 cut(s) 518
HpyCH4III ACNGT 4 cut(s) 326, 458, 784, 805
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 7 cut(s) 37, 84, 454, 693, 725, 734, 744
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 43, 81, 229, 629, 731, 744
HpyF3I CTNAG 4 cut(s) 318, 381, 428, 477
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 6 cut(s) 75, 130, 169, 500, 618, 725
HspAI GCGC 2 cut(s) 75, 336
KasI GGCGCC 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 2 cut(s) 196, 328
Lsp1109I GCAGC 3 cut(s) 438, 705, 756
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 388, 659, 667
MalI GATC 2 cut(s) 198, 330
MboI GATC 2 cut(s) 196, 328
MboII GAAGA 7 cut(s) 139, 246, 297, 365, 427, 621, 821
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 2 cut(s) 31, 484
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 186, 513
MnlI CCTC 9 cut(s) 42, 149, 155, 169, 313, 474, 547, 567, 673
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 1 cut(s) 311
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 7 cut(s) 21, 43, 81, 229, 629, 731, 744
NarI GGCGCC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 126
NdeII GATC 2 cut(s) 196, 328
NlaIII CATG 6 cut(s) 75, 130, 169, 500, 618, 725
NlaIV GGNNCC 5 cut(s) 124, 302, 315, 337, 603
NmuCI GTSAC 1 cut(s) 659
NspI RCATGY 5 cut(s) 75, 169, 500, 618, 725
PaqCI CACCTGC 2 cut(s) 28, 517
PciI ACATGT 3 cut(s) 165, 496, 614
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 310
PkrI GCNGC 4 cut(s) 231, 453, 695, 746
PleI GAGTC 1 cut(s) 696
PluTI GGCGCC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 696
PpuMI RGGWCCY 1 cut(s) 122
PscI ACATGT 3 cut(s) 165, 496, 614
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 309
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 5 cut(s) 124, 302, 315, 337, 603
PspPI GGNCC 3 cut(s) 122, 300, 313
PspPPI RGGWCCY 1 cut(s) 122
PstNI CAGNNNCTG 2 cut(s) 310, 368
RsaI GTAC 3 cut(s) 269, 308, 570
RsaNI GTAC 3 cut(s) 268, 307, 569
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 4 cut(s) 230, 452, 694, 745
Sau3AI GATC 2 cut(s) 196, 328
Sau96I GGNCC 3 cut(s) 122, 300, 313
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 1 cut(s) 311
SfoI GGCGCC 1 cut(s) 337
SinI GGWCC 3 cut(s) 122, 300, 313
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
Sse9I AATT 2 cut(s) 31, 484
SsiI CCGC 4 cut(s) 103, 142, 229, 404
SspDI GGCGCC 1 cut(s) 335
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 4 cut(s) 326, 458, 784, 805
TaiI ACGT 1 cut(s) 345
TasI AATT 2 cut(s) 31, 484
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 232
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 3 cut(s) 451, 693, 744
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 2 cut(s) 33, 809
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 3 cut(s) 122, 300, 313
XapI RAATTY 2 cut(s) 31, 484
XceI RCATGY 5 cut(s) 75, 169, 500, 618, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.