Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004208

Physical Location & Seq

Genomic Coordinates Reverse (-)

6912493 .. 6917847

Gene Structure 5355 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1040800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGTAGAAGTTGCAGTTCCCACCCGCGTTCATATCAGACCCCTTCTTTCTGTAAGCCCACATCTTCCAATTCGTCGTAATATAGTGTCAACAAATACAAAAGCATGCAAGATTCGTGGAGTTGCAGCTTCTTCTTCTTCTTCTTCTTCTTCTTCCGCTGGCACTTGTGGCTTACACGCTTTTGCTCATTGGGTCTCCTTATTTTTCAATCAAGTTAGCCTATCCGACCCTGTTATTGCTGTTGTTGATGGGGAAGGAGTAAGTGAGCACCGCGGACAGTTATTACCATCCTATAAAGCACATAGGTGGAAATTCACAAGACAGTTTCCAAGGGGGCATGCTGGAAGGTCACATGGAGTCATCACCAATGTTCTTCAAAAATGCAATGTCATAAAAGTAGAACATCACGAAACTGATGATGTTATAGCTACACTTGTGGGGCAAGTTATTCAAACAGGATATTGGGTGGTGATTGCCTCTCCTGATAAAGATTTTAAGCAATTGCTTTCGGAAGATGTCCAGCTTGTTATGCCCCTGGACGAGTTAGAACGCTGGTCGTTTTACACTCTTAAGCACTACATAGCTCAGTATAACTGTGATCCATGCTGTGATTTGAGCCTTAGGTGCTTTGTGGGTAATCACGCTGATGGTGTTCCTGGAATCCAACATCTAGCTCCTGGATTTGGTCAGAAGACTGCCCTAAAGCTCTTGAAAAAGCATGGTTCATTGGAAAATCTACTAAACACAGCTGCAGTCAGAACTGTGGGCAGACAGTATGCTCAAGATGCTCTAACAAAACATGCTGATTACCTAAGGAGGAACCACGAGATTCTTTCCCTTAGGAGGGATGTTGATGTTAAACTTCGAGAGGAGTGGTTGGTTACGAGAGACACAAGCAATGATTCAGCAACTCTGTCTAACTTCTATAAATTCTTGGAAGAAACCGGGTATTTCAGTTGTCAAAGAGCATCTATTTTGTCCTTCGGTGGCGAAGAGAAACTCGGCTGTGGTAGATTGCAATGTGGTCAGATGGTAATAGTGCATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

5_3_exonuc 5_3_exonuc_N

Protein Analysis

350

Amino Acids

38.92

Weight (kDa)

8.63

Isoelectric Point (pI)

40.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
5_3_exonuc_N	PF02739	49 - 182	3.2e-16	5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc	PF01367	209 - 296	5.9e-13	5'-3' exonuclease, C-terminal SAM fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004208)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34380 AT1G34380 AT1G34380
fragaria_vesca	FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160
malus_domestica	MD05G1040800.v1.1 MD10G1046800.v1.1
prunus_persica	Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1
pyrus_communis	pycom05g03340 pycom05g03350 pycom10g03310
rosa_chinensis	RchiOBHm_Chr6g0272141
rosa_laevigata	RLG00000013698
rosa_multiflora	Rmu_co7960774.1_g000001 Rmu_sc0004606.1_g000001 Rmu_sc0008168.1_g000006 Rmu_sc0013301.1_g000010 Rmu_sc0027055.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196560
rosa_rugosa	Rorug06G0066000
rosa_samantha	Rh6AG183200 Rh6BG183900 Rh6CG184500 Rh6DG176200
rosa_wichuraiana	Rw6G015770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	27, 273
AciI	CCGC	4 cut(s)	25, 156, 271, 273
AclWI	GGATC	1 cut(s)	593
AcsI	RAATTY	2 cut(s)	311, 929
AfiI	CCNNNNNNNGG	3 cut(s)	683, 843, 844
AflII	CTTAAG	1 cut(s)	569
AgsI	TTSAA	4 cut(s)	208, 377, 452, 712
AjnI	CCWGG	3 cut(s)	534, 655, 676
AleI	CACNNNNGTG	1 cut(s)	304
AluBI	AGCT	7 cut(s)	128, 428, 523, 584, 674, 706, 749
AluI	AGCT	7 cut(s)	128, 428, 523, 584, 674, 706, 749
Alw21I	GWGCWC	1 cut(s)	270
Alw26I	GTCTC	2 cut(s)	199, 882
AlwI	GGATC	1 cut(s)	593
ApeKI	GCWGC	2 cut(s)	125, 749
ApoI	RAATTY	2 cut(s)	311, 929
AsuC2I	CCSGG	1 cut(s)	946
AsuHPI	GGTGA	2 cut(s)	355, 481
AxyI	CCTNAGG	3 cut(s)	620, 812, 839
BauI	CACGAG	1 cut(s)	824
BbsI	GAAGAC	1 cut(s)	698
Bbv12I	GWGCWC	1 cut(s)	270
BbvI	GCAGC	2 cut(s)	137, 736
BccI	CCATC	4 cut(s)	242, 295, 641, 1024
BciT130I	CCWGG	3 cut(s)	536, 657, 678
BcnI	CCSGG	1 cut(s)	946
BcoDI	GTCTC	2 cut(s)	199, 882
BfaI	CTAG	1 cut(s)	671
BfmI	CTRYAG	1 cut(s)	750
BfrI	CTTAAG	1 cut(s)	569
BisI	GCNGC	2 cut(s)	126, 750
BlsI	GCNGC	2 cut(s)	127, 751
Bme1390I	CCNGG	4 cut(s)	536, 657, 678, 946
BmiI	GGNNCC	1 cut(s)	821
BmrFI	CCNGG	4 cut(s)	536, 657, 678, 946
BmsI	GCATC	2 cut(s)	775, 977
BpiI	GAAGAC	1 cut(s)	698
BpuEI	CTTGAG	1 cut(s)	765
BpuMI	CCSGG	1 cut(s)	946
BsaI	GGTCTC	1 cut(s)	199
BsaJI	CCNNGG	3 cut(s)	271, 329, 534
Bsc4I	CCNNNNNNNGG	3 cut(s)	683, 843, 844
Bse21I	CCTNAGG	3 cut(s)	620, 812, 839
Bse3DI	GCAATG	3 cut(s)	391, 904, 1025
BseBI	CCWGG	3 cut(s)	536, 657, 678
BseDI	CCNNGG	3 cut(s)	271, 329, 534
BseGI	GGATG	2 cut(s)	287, 853
BseLI	CCNNNNNNNGG	3 cut(s)	683, 843, 844
BseMI	GCAATG	3 cut(s)	391, 904, 1025
BseMII	CTCAG	1 cut(s)	599
BseRI	GAGGAG	1 cut(s)	884
BseXI	GCAGC	2 cut(s)	137, 736
Bsh1236I	CGCG	2 cut(s)	27, 273
BsiHKAI	GWGCWC	1 cut(s)	270
BsiSI	CCGG	1 cut(s)	945
BslI	CCNNNNNNNGG	3 cut(s)	683, 843, 844
BsmAI	GTCTC	2 cut(s)	199, 882
Bso31I	GGTCTC	1 cut(s)	199
Bsp1286I	GDGCHC	1 cut(s)	270
Bsp143I	GATC	1 cut(s)	598
BspACI	CCGC	4 cut(s)	25, 156, 271, 273
BspCNI	CTCAG	1 cut(s)	598
BspFNI	CGCG	2 cut(s)	27, 273
BspLI	GGNNCC	1 cut(s)	821
BspMAI	CTGCAG	1 cut(s)	754
BspPI	GGATC	1 cut(s)	593
BspTI	CTTAAG	1 cut(s)	569
BspTNI	GGTCTC	1 cut(s)	199
BsrDI	GCAATG	3 cut(s)	391, 904, 1025
BssECI	CCNNGG	3 cut(s)	271, 329, 534
BssMI	GATC	1 cut(s)	598
BssSI	CACGAG	1 cut(s)	824
BssT1I	CCWWGG	1 cut(s)	329
Bst2BI	CACGAG	1 cut(s)	824
Bst2UI	CCWGG	3 cut(s)	536, 657, 678
Bst4CI	ACNGT	5 cut(s)	279, 324, 596, 763, 774
Bst6I	CTCTTC	1 cut(s)	987
BstAFI	CTTAAG	1 cut(s)	569
BstC8I	GCNNGC	3 cut(s)	106, 160, 339
BstDEI	CTNAG	4 cut(s)	585, 620, 812, 839
BstDSI	CCRYGG	1 cut(s)	271
BstF5I	GGATG	2 cut(s)	287, 853
BstFNI	CGCG	2 cut(s)	27, 273
BstKTI	GATC	1 cut(s)	601
BstMAI	GTCTC	2 cut(s)	199, 882
BstMBI	GATC	1 cut(s)	598
BstMWI	GCNNNNNNNGC	4 cut(s)	168, 529, 624, 785
BstNI	CCWGG	3 cut(s)	536, 657, 678
BstNSI	RCATGY	3 cut(s)	108, 341, 803
BstSCI	CCNGG	4 cut(s)	534, 655, 676, 944
BstSFI	CTRYAG	1 cut(s)	750
BstUI	CGCG	2 cut(s)	27, 273
BstV1I	GCAGC	2 cut(s)	137, 736
BstV2I	GAAGAC	1 cut(s)	698
Bsu36I	CCTNAGG	3 cut(s)	620, 812, 839
BtgI	CCRYGG	1 cut(s)	271
BtsCI	GGATG	2 cut(s)	287, 853
Cac8I	GCNNGC	3 cut(s)	106, 160, 339
Cfr42I	CCGCGG	1 cut(s)	274
CspCI	CAANNNNNGTGG	2 cut(s)	97, 132
CviAII	CATG	7 cut(s)	105, 338, 353, 603, 719, 800, 1043
DdeI	CTNAG	4 cut(s)	585, 620, 812, 839
DpnI	GATC	1 cut(s)	600
DpnII	GATC	1 cut(s)	598
Eam1104I	CTCTTC	1 cut(s)	987
EarI	CTCTTC	1 cut(s)	987
Eco130I	CCWWGG	1 cut(s)	329
Eco31I	GGTCTC	1 cut(s)	199
Eco81I	CCTNAGG	3 cut(s)	620, 812, 839
EcoRII	CCWGG	3 cut(s)	534, 655, 676
EcoT14I	CCWWGG	1 cut(s)	329
ErhI	CCWWGG	1 cut(s)	329
FaeI	CATG	7 cut(s)	108, 341, 356, 606, 722, 803, 1046
FatI	CATG	7 cut(s)	104, 337, 352, 602, 718, 799, 1042
FauI	CCCGC	1 cut(s)	32
Fnu4HI	GCNGC	2 cut(s)	126, 750
FokI	GGATG	2 cut(s)	274, 860
Fsp4HI	GCNGC	2 cut(s)	126, 750
FspBI	CTAG	1 cut(s)	671
GluI	GCNGC	2 cut(s)	126, 750
HapII	CCGG	1 cut(s)	945
Hin1II	CATG	7 cut(s)	108, 341, 356, 606, 722, 803, 1046
HincII	GTYRAC	1 cut(s)	90
HindII	GTYRAC	1 cut(s)	90
HinfI	GANTC	5 cut(s)	112, 357, 660, 829, 902
HpaII	CCGG	1 cut(s)	945
HphI	GGTGA	2 cut(s)	355, 481
Hpy166II	GTNNAC	1 cut(s)	90
Hpy188I	TCNGA	6 cut(s)	38, 226, 511, 690, 758, 1029
Hpy188III	TCNNGA	5 cut(s)	407, 482, 709, 782, 866
Hpy8I	GTNNAC	1 cut(s)	90
Hpy99I	CGWCG	1 cut(s)	78
HpyAV	CCTTC	4 cut(s)	53, 248, 339, 991
HpyCH4III	ACNGT	5 cut(s)	279, 324, 596, 763, 774
HpyCH4V	TGCA	7 cut(s)	14, 108, 125, 384, 752, 1018, 1042
HpyF10VI	GCNNNNNNNGC	4 cut(s)	168, 529, 624, 785
HpyF3I	CTNAG	4 cut(s)	585, 620, 812, 839
Hsp92II	CATG	7 cut(s)	108, 341, 356, 606, 722, 803, 1046
KspI	CCGCGG	1 cut(s)	274
Kzo9I	GATC	1 cut(s)	598
LmnI	GCTCC	1 cut(s)	679
Lsp1109I	GCAGC	2 cut(s)	137, 736
LweI	GCATC	2 cut(s)	775, 977
MaeI	CTAG	1 cut(s)	671
MaeIII	GTNAC	2 cut(s)	348, 880
MalI	GATC	1 cut(s)	600
MboI	GATC	1 cut(s)	598
MfeI	CAATTG	1 cut(s)	500
MhlI	GDGCHC	1 cut(s)	270
MluCI	AATT	4 cut(s)	69, 311, 500, 929
MlyI	GAGTC	1 cut(s)	366
MmeI	TCCRAC	2 cut(s)	249, 688
MnlI	CCTC	4 cut(s)	487, 810, 837, 862
MseI	TTAA	3 cut(s)	495, 570, 858
MslI	CAYNNNNRTG	2 cut(s)	304, 645
MspA1I	CMGCKG	3 cut(s)	158, 273, 749
MspCI	CTTAAG	1 cut(s)	569
MspI	CCGG	1 cut(s)	945
MspR9I	CCNGG	4 cut(s)	536, 657, 678, 946
MunI	CAATTG	1 cut(s)	500
MvaI	CCWGG	3 cut(s)	536, 657, 678
MvnI	CGCG	2 cut(s)	27, 273
MwoI	GCNNNNNNNGC	4 cut(s)	168, 529, 624, 785
NciI	CCSGG	1 cut(s)	946
NdeII	GATC	1 cut(s)	598
NlaIII	CATG	7 cut(s)	108, 341, 356, 606, 722, 803, 1046
NlaIV	GGNNCC	1 cut(s)	821
NmeAIII	GCCGAG	1 cut(s)	981
NmuCI	GTSAC	1 cut(s)	348
NspI	RCATGY	3 cut(s)	108, 341, 803
OliI	CACNNNNGTG	1 cut(s)	304
PaeI	GCATGC	2 cut(s)	108, 341
PfeI	GAWTC	4 cut(s)	112, 660, 829, 902
PfoI	TCCNGGA	2 cut(s)	655, 676
PkrI	GCNGC	2 cut(s)	127, 751
PleI	GAGTC	1 cut(s)	365
PpsI	GAGTC	1 cut(s)	365
Psp6I	CCWGG	3 cut(s)	534, 655, 676
PspGI	CCWGG	3 cut(s)	534, 655, 676
PspN4I	GGNNCC	1 cut(s)	821
PstI	CTGCAG	1 cut(s)	754
PvuII	CAGCTG	1 cut(s)	749
RseI	CAYNNNNRTG	2 cut(s)	304, 645
SacII	CCGCGG	1 cut(s)	274
SaqAI	TTAA	3 cut(s)	495, 570, 858
SatI	GCNGC	2 cut(s)	126, 750
Sau3AI	GATC	1 cut(s)	598
SchI	GAGTC	1 cut(s)	366
ScrFI	CCNGG	4 cut(s)	536, 657, 678, 946
SduI	GDGCHC	1 cut(s)	270
SfaNI	GCATC	2 cut(s)	775, 977
SfcI	CTRYAG	1 cut(s)	750
Sfr303I	CCGCGG	1 cut(s)	274
SgrBI	CCGCGG	1 cut(s)	274
SmiMI	CAYNNNNRTG	2 cut(s)	304, 645
SmlI	CTYRAG	2 cut(s)	569, 780
SmoI	CTYRAG	2 cut(s)	569, 780
SphI	GCATGC	2 cut(s)	108, 341
Sse9I	AATT	4 cut(s)	69, 311, 500, 929
SsiI	CCGC	4 cut(s)	25, 156, 271, 273
SspMI	CTAG	1 cut(s)	671
StyD4I	CCNGG	4 cut(s)	534, 655, 676, 944
StyI	CCWWGG	1 cut(s)	329
TaaI	ACNGT	5 cut(s)	279, 324, 596, 763, 774
TaqI	TCGA	1 cut(s)	865
TasI	AATT	4 cut(s)	69, 311, 500, 929
TfiI	GAWTC	4 cut(s)	112, 660, 829, 902
Tru1I	TTAA	3 cut(s)	495, 570, 858
Tru9I	TTAA	3 cut(s)	495, 570, 858
TseFI	GTSAC	1 cut(s)	348
TseI	GCWGC	2 cut(s)	125, 749
Tsp45I	GTSAC	1 cut(s)	348
TspDTI	ATGAA	2 cut(s)	20, 714
Vha464I	CTTAAG	1 cut(s)	569
XapI	RAATTY	2 cut(s)	311, 929
XceI	RCATGY	3 cut(s)	108, 341, 803
XspI	CTAG	1 cut(s)	671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.