Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004208

Physical Location & Seq

Genomic Coordinates Reverse (-)

6266545 .. 6272616

Gene Structure 6072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1046800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGGTAGAAGTTGCAGTTCCTACCGGCGTTCATATCAGACCCTTTCTTTCTGTAAGCCCACAGCATCCAATTCGTCGCACAATAGTGGTAACAAAGACAAAACCATGGAAGATTCGTGGAGTTGCAGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTACTACTAGTACTTGTGGGTATAAACAAACGGCTGATGATGGACAACAAGTAGTGTTGCCAAACAAGGCAAGCAAGAAAAGGGTCTTCTTTTTAGACGTCAATCCTCTCTGCTATGCAGGAAGCAGACCCAGCTTACACGCTTTTGCTCATTGGGTCTCCTTATTTTTCAATCAAGTTAGCCTATCTGACCCTGTTATTGCTGTTGTTGATGGCGAAGGAGGAAGTGAGCACCGCAGACAGTTATTACCATCTTATAAAGCACACAGGTGGAAATTCACAAGACAGTTTTCAAAGGGGCATGTTGGAAGGTCACATGGAGTCATCACCAATGTTCTTAGAAAATGCAATGTGCCTGTCATAAAAGTAGAAGGTCATGAAGCTGATGATGTTATAGCTACACTTGTGGGGCAAGTTCTTCAAACAGGATATCGGGTGGTGATTGCCTCACCTGATAAAGATTTTAAGCAATTGCTTTCGGAAGATGTCCAGCTTGTTGTGCCCCTGGATGAGTTAGAACGCTGGTCGTTTTACACTCTTAAGCACTACCTAGCTCAGTATAACTGTGATCCATGCTGTGATTTGAGCCTTAGGTGCATTGTGGGTGATCACACTGATGGTGTTCCTGGAATCCAACATCTAGCTCCTGGATTTGGTCAGAAGACTGCCCTAAAGCTATTGAAAAAGCACGGTTCATTGGAAAACCTACTAAACACAGCTGCAGTCAGAACTGTGGGCAGACAGTATGCTCAAGATGCTCTAACAAAACATGCTGATTACCTAAGGAGGAACTACGAGATTCTTTCCCTTAGGAGGGATGTCGATGTTCAGCTTAGAGAAGAGTGGTTGCTTACCAGAGATACAAACAACGATTCTACAACTCTGTCTAACTTCTATAAATTCTTGGAAGAAACCCGGGATTTCCGTCATCAAAGAGCATCTGGATGTGCTACCATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

5_3_exonuc 5_3_exonuc_N

Protein Analysis

377

Amino Acids

42.06

Weight (kDa)

9.25

Isoelectric Point (pI)

44.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
5_3_exonuc_N	PF02739	106 - 226	8.8e-22	5'-3' exonuclease, N-terminal resolvase-like domain
5_3_exonuc	PF01367	256 - 343	3.4e-14	5'-3' exonuclease, C-terminal SAM fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004208)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34380 AT1G34380 AT1G34380
fragaria_vesca	FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160 FvH4_2g12160
malus_domestica	MD05G1040800.v1.1 MD10G1046800.v1.1
prunus_persica	Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1 Prupe.8G055700_v2.0.a1
pyrus_communis	pycom05g03340 pycom05g03350 pycom10g03310
rosa_chinensis	RchiOBHm_Chr6g0272141
rosa_laevigata	RLG00000013698
rosa_multiflora	Rmu_co7960774.1_g000001 Rmu_sc0004606.1_g000001 Rmu_sc0008168.1_g000006 Rmu_sc0013301.1_g000010 Rmu_sc0027055.1_g000001
rosa_roxburghii	Rroxscaffold_7G00196560
rosa_rugosa	Rorug06G0066000
rosa_samantha	Rh6AG183200 Rh6BG183900 Rh6CG184500 Rh6DG176200
rosa_wichuraiana	Rw6G015770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	429
AatII	GACGTC	1 cut(s)	273
AciI	CCGC	1 cut(s)	406
AclWI	GGATC	1 cut(s)	734
AcsI	RAATTY	2 cut(s)	446, 1070
AcyI	GRCGYC	1 cut(s)	270
AfaI	GTAC	1 cut(s)	184
AfiI	CCNNNNNNNGG	3 cut(s)	824, 984, 985
AflII	CTTAAG	1 cut(s)	710
AgsI	TTSAA	4 cut(s)	343, 465, 593, 853
AhlI	ACTAGT	1 cut(s)	179
AjnI	CCWGG	3 cut(s)	675, 796, 817
AleI	CACNNNNGTG	2 cut(s)	83, 439
Alw21I	GWGCWC	1 cut(s)	405
Alw26I	GTCTC	1 cut(s)	334
AlwI	GGATC	1 cut(s)	734
Ama87I	CYCGRG	1 cut(s)	1086
ApeKI	GCWGC	2 cut(s)	125, 890
ApoI	RAATTY	2 cut(s)	446, 1070
AsuC2I	CCSGG	2 cut(s)	1087, 1088
AsuHPI	GGTGA	4 cut(s)	490, 612, 622, 788
AvaI	CYCGRG	1 cut(s)	1086
AxyI	CCTNAGG	3 cut(s)	761, 953, 980
BaeGI	GKGCMC	1 cut(s)	675
BbsI	GAAGAC	2 cut(s)	250, 839
Bbv12I	GWGCWC	1 cut(s)	405
BbvI	GCAGC	2 cut(s)	137, 877
BccI	CCATC	4 cut(s)	206, 377, 430, 782
BceAI	ACGGC	1 cut(s)	219
BcgI	CGANNNNNNTGC	2 cut(s)	53, 87
BciT130I	CCWGG	3 cut(s)	677, 798, 819
BclI	TGATCA	1 cut(s)	778
BcnI	CCSGG	2 cut(s)	1087, 1088
BcoDI	GTCTC	1 cut(s)	334
BcuI	ACTAGT	1 cut(s)	179
BfaI	CTAG	3 cut(s)	180, 722, 812
BfmI	CTRYAG	1 cut(s)	891
BfrI	CTTAAG	1 cut(s)	710
BisI	GCNGC	2 cut(s)	126, 891
BlsI	GCNGC	2 cut(s)	127, 892
BmcAI	AGTACT	1 cut(s)	184
Bme1390I	CCNGG	5 cut(s)	677, 798, 819, 1087, 1088
BmeT110I	CYCGRG	1 cut(s)	1086
BmrFI	CCNGG	5 cut(s)	677, 798, 819, 1087, 1088
BmsI	GCATC	3 cut(s)	73, 916, 1118
BpiI	GAAGAC	2 cut(s)	250, 839
BpuEI	CTTGAG	1 cut(s)	906
BpuMI	CCSGG	2 cut(s)	1087, 1088
BsaHI	GRCGYC	1 cut(s)	270
BsaI	GGTCTC	1 cut(s)	334
BsaJI	CCNNGG	3 cut(s)	104, 675, 1086
Bsc4I	CCNNNNNNNGG	3 cut(s)	824, 984, 985
Bse118I	RCCGGY	1 cut(s)	23
Bse21I	CCTNAGG	3 cut(s)	761, 953, 980
Bse3DI	GCAATG	1 cut(s)	526
BseBI	CCWGG	3 cut(s)	677, 798, 819
BseDI	CCNNGG	3 cut(s)	104, 675, 1086
BseGI	GGATG	4 cut(s)	64, 685, 994, 1121
BseLI	CCNNNNNNNGG	3 cut(s)	824, 984, 985
BseMI	GCAATG	1 cut(s)	526
BseMII	CTCAG	1 cut(s)	740
BseSI	GKGCMC	1 cut(s)	675
BseXI	GCAGC	2 cut(s)	137, 877
BseYI	CCCAGC	1 cut(s)	302
BsiHKAI	GWGCWC	1 cut(s)	405
BsiHKCI	CYCGRG	1 cut(s)	1086
BsiSI	CCGG	2 cut(s)	24, 1087
BslI	CCNNNNNNNGG	3 cut(s)	824, 984, 985
BsmAI	GTCTC	1 cut(s)	334
Bso31I	GGTCTC	1 cut(s)	334
BsoBI	CYCGRG	1 cut(s)	1086
Bsp1286I	GDGCHC	2 cut(s)	405, 675
Bsp143I	GATC	2 cut(s)	739, 778
Bsp19I	CCATGG	1 cut(s)	104
BspACI	CCGC	1 cut(s)	406
BspCNI	CTCAG	1 cut(s)	739
BspHI	TCATGA	1 cut(s)	547
BspMAI	CTGCAG	1 cut(s)	895
BspPI	GGATC	1 cut(s)	734
BspTI	CTTAAG	1 cut(s)	710
BspTNI	GGTCTC	1 cut(s)	334
BsrDI	GCAATG	1 cut(s)	526
BsrFI	RCCGGY	1 cut(s)	23
BssAI	RCCGGY	1 cut(s)	23
BssECI	CCNNGG	3 cut(s)	104, 675, 1086
BssMI	GATC	2 cut(s)	739, 778
BssNI	GRCGYC	1 cut(s)	270
BssT1I	CCWWGG	1 cut(s)	104
Bst2UI	CCWGG	3 cut(s)	677, 798, 819
Bst4CI	ACNGT	6 cut(s)	414, 459, 737, 863, 904, 915
Bst6I	CTCTTC	1 cut(s)	1005
BstACI	GRCGYC	1 cut(s)	270
BstAFI	CTTAAG	1 cut(s)	710
BstC8I	GCNNGC	1 cut(s)	244
BstDEI	CTNAG	6 cut(s)	509, 726, 761, 953, 980, 1004
BstDSI	CCRYGG	1 cut(s)	104
BstF5I	GGATG	4 cut(s)	64, 685, 994, 1121
BstKTI	GATC	2 cut(s)	742, 781
BstMAI	GTCTC	1 cut(s)	334
BstMBI	GATC	2 cut(s)	739, 778
BstMWI	GCNNNNNNNGC	4 cut(s)	303, 670, 765, 926
BstNI	CCWGG	3 cut(s)	677, 798, 819
BstNSI	RCATGY	2 cut(s)	476, 944
BstSCI	CCNGG	5 cut(s)	675, 796, 817, 1085, 1086
BstSFI	CTRYAG	1 cut(s)	891
BstSLI	GKGCMC	1 cut(s)	675
BstV1I	GCAGC	2 cut(s)	137, 877
BstV2I	GAAGAC	2 cut(s)	250, 839
Bsu36I	CCTNAGG	3 cut(s)	761, 953, 980
BtgI	CCRYGG	1 cut(s)	104
BtsCI	GGATG	4 cut(s)	64, 685, 994, 1121
BtsIMutI	CAGTG	1 cut(s)	783
Cac8I	GCNNGC	1 cut(s)	244
CciI	TCATGA	1 cut(s)	547
Cfr10I	RCCGGY	1 cut(s)	23
Cfr9I	CCCGGG	1 cut(s)	1086
Csp6I	GTAC	1 cut(s)	183
CviAII	CATG	6 cut(s)	105, 473, 488, 548, 744, 941
CviQI	GTAC	1 cut(s)	183
DdeI	CTNAG	6 cut(s)	509, 726, 761, 953, 980, 1004
DpnI	GATC	2 cut(s)	741, 780
DpnII	GATC	2 cut(s)	739, 778
Eam1104I	CTCTTC	1 cut(s)	1005
EarI	CTCTTC	1 cut(s)	1005
Eco130I	CCWWGG	1 cut(s)	104
Eco31I	GGTCTC	1 cut(s)	334
Eco32I	GATATC	1 cut(s)	602
Eco81I	CCTNAGG	3 cut(s)	761, 953, 980
Eco88I	CYCGRG	1 cut(s)	1086
EcoRII	CCWGG	3 cut(s)	675, 796, 817
EcoRV	GATATC	1 cut(s)	602
EcoT14I	CCWWGG	1 cut(s)	104
ErhI	CCWWGG	1 cut(s)	104
FaeI	CATG	6 cut(s)	108, 476, 491, 551, 747, 944
FatI	CATG	6 cut(s)	104, 472, 487, 547, 743, 940
FbaI	TGATCA	1 cut(s)	778
Fnu4HI	GCNGC	2 cut(s)	126, 891
FokI	GGATG	3 cut(s)	51, 692, 1001
Fsp4HI	GCNGC	2 cut(s)	126, 891
FspBI	CTAG	3 cut(s)	180, 722, 812
GluI	GCNGC	2 cut(s)	126, 891
GsaI	CCCAGC	1 cut(s)	306
HapII	CCGG	2 cut(s)	24, 1087
Hin1I	GRCGYC	1 cut(s)	270
Hin1II	CATG	6 cut(s)	108, 476, 491, 551, 747, 944
HinfI	GANTC	5 cut(s)	112, 492, 801, 970, 1043
HpaII	CCGG	2 cut(s)	24, 1087
HphI	GGTGA	4 cut(s)	490, 612, 622, 788
Hpy188I	TCNGA	5 cut(s)	38, 361, 652, 831, 899
Hpy188III	TCNNGA	3 cut(s)	548, 923, 1113
Hpy99I	CGWCG	1 cut(s)	78
HpyAV	CCTTC	3 cut(s)	383, 474, 536
HpyCH4III	ACNGT	6 cut(s)	414, 459, 737, 863, 904, 915
HpyCH4IV	ACGT	1 cut(s)	270
HpyCH4V	TGCA	6 cut(s)	14, 125, 290, 519, 768, 893
HpyF10VI	GCNNNNNNNGC	4 cut(s)	303, 670, 765, 926
HpyF3I	CTNAG	6 cut(s)	509, 726, 761, 953, 980, 1004
HpySE526I	ACGT	1 cut(s)	270
Hsp92I	GRCGYC	1 cut(s)	270
Hsp92II	CATG	6 cut(s)	108, 476, 491, 551, 747, 944
Ksp22I	TGATCA	1 cut(s)	778
Kzo9I	GATC	2 cut(s)	739, 778
LmnI	GCTCC	1 cut(s)	820
Lsp1109I	GCAGC	2 cut(s)	137, 877
LweI	GCATC	3 cut(s)	73, 916, 1118
MaeI	CTAG	3 cut(s)	180, 722, 812
MaeII	ACGT	1 cut(s)	270
MaeIII	GTNAC	2 cut(s)	88, 483
MalI	GATC	2 cut(s)	741, 780
MboI	GATC	2 cut(s)	739, 778
MfeI	CAATTG	1 cut(s)	641
MhlI	GDGCHC	2 cut(s)	405, 675
MluCI	AATT	4 cut(s)	69, 446, 641, 1070
MlyI	GAGTC	1 cut(s)	501
MmeI	TCCRAC	2 cut(s)	457, 829
MnlI	CCTC	5 cut(s)	288, 386, 628, 951, 978
MseI	TTAA	2 cut(s)	636, 711
MslI	CAYNNNNRTG	4 cut(s)	83, 439, 786, 1114
MspA1I	CMGCKG	1 cut(s)	890
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	2 cut(s)	24, 1087
MspR9I	CCNGG	5 cut(s)	677, 798, 819, 1087, 1088
MunI	CAATTG	1 cut(s)	641
MvaI	CCWGG	3 cut(s)	677, 798, 819
MwoI	GCNNNNNNNGC	4 cut(s)	303, 670, 765, 926
NciI	CCSGG	2 cut(s)	1087, 1088
NcoI	CCATGG	1 cut(s)	104
NdeII	GATC	2 cut(s)	739, 778
NlaIII	CATG	6 cut(s)	108, 476, 491, 551, 747, 944
NmuCI	GTSAC	1 cut(s)	483
NspI	RCATGY	2 cut(s)	476, 944
OliI	CACNNNNGTG	2 cut(s)	83, 439
PagI	TCATGA	1 cut(s)	547
PfeI	GAWTC	4 cut(s)	112, 801, 970, 1043
PfoI	TCCNGGA	2 cut(s)	796, 817
PkrI	GCNGC	2 cut(s)	127, 892
PleI	GAGTC	1 cut(s)	500
PpsI	GAGTC	1 cut(s)	500
PsiI	TTATAA	1 cut(s)	429
Psp6I	CCWGG	3 cut(s)	675, 796, 817
PspFI	CCCAGC	1 cut(s)	302
PspGI	CCWGG	3 cut(s)	675, 796, 817
PstI	CTGCAG	1 cut(s)	895
PvuII	CAGCTG	1 cut(s)	890
RsaI	GTAC	1 cut(s)	184
RsaNI	GTAC	1 cut(s)	183
RseI	CAYNNNNRTG	4 cut(s)	83, 439, 786, 1114
SaqAI	TTAA	2 cut(s)	636, 711
SatI	GCNGC	2 cut(s)	126, 891
Sau3AI	GATC	2 cut(s)	739, 778
ScaI	AGTACT	1 cut(s)	184
SchI	GAGTC	1 cut(s)	501
ScrFI	CCNGG	5 cut(s)	677, 798, 819, 1087, 1088
SduI	GDGCHC	2 cut(s)	405, 675
SfaNI	GCATC	3 cut(s)	73, 916, 1118
SfcI	CTRYAG	1 cut(s)	891
SmaI	CCCGGG	1 cut(s)	1088
SmiMI	CAYNNNNRTG	4 cut(s)	83, 439, 786, 1114
SmlI	CTYRAG	2 cut(s)	710, 921
SmoI	CTYRAG	2 cut(s)	710, 921
SpeI	ACTAGT	1 cut(s)	179
Sse9I	AATT	4 cut(s)	69, 446, 641, 1070
SsiI	CCGC	1 cut(s)	406
SspMI	CTAG	3 cut(s)	180, 722, 812
StyD4I	CCNGG	5 cut(s)	675, 796, 817, 1085, 1086
StyI	CCWWGG	1 cut(s)	104
TaaI	ACNGT	6 cut(s)	414, 459, 737, 863, 904, 915
TaiI	ACGT	1 cut(s)	273
TaqI	TCGA	1 cut(s)	993
TasI	AATT	4 cut(s)	69, 446, 641, 1070
TatI	WGTACW	1 cut(s)	182
TfiI	GAWTC	4 cut(s)	112, 801, 970, 1043
Tru1I	TTAA	2 cut(s)	636, 711
Tru9I	TTAA	2 cut(s)	636, 711
TscAI	CASTG	1 cut(s)	790
TseFI	GTSAC	1 cut(s)	483
TseI	GCWGC	2 cut(s)	125, 890
Tsp45I	GTSAC	1 cut(s)	483
TspDTI	ATGAA	3 cut(s)	20, 564, 855
TspGWI	ACGGA	1 cut(s)	1085
TspMI	CCCGGG	1 cut(s)	1086
TspRI	CASTG	1 cut(s)	790
Vha464I	CTTAAG	1 cut(s)	710
XapI	RAATTY	2 cut(s)	446, 1070
XceI	RCATGY	2 cut(s)	476, 944
XmaI	CCCGGG	1 cut(s)	1086
XspI	CTAG	3 cut(s)	180, 722, 812
ZraI	GACGTC	1 cut(s)	271
ZrmI	AGTACT	1 cut(s)	184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.