Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002754

Physical Location & Seq

Genomic Coordinates Forward (+)

8624874 .. 8628021

Gene Structure 3148 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1049700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGGCCACCGCCGCCTTGCTCCGCTCGATGCGCCGACGTGATGTCGCATCGGCTCCTCTCTGCGCCTATCGATCCTTGAACAGCGCATCTAAGTCATCCCATCTTGCTCAAAAATGGGCAAGCTTGGCAAGACCTTTCAGTTCCAAACCTGCTGGAAATGATGTTATTGGCATTGACTTGGGTACAACCAACTCATGCGTGGCCGTGATGGAAGGAAAGAACCCTAAAGTGATTGAGAATTCCGAAGGAGCTCGAACCACACCATCAGTAGTTGCATTCAACCAGAAAGGAGAACTATTGGTTGGTACTCCAGCAAAACGTCAAGCTGTTACCAACCCCACAAACACAGTTTTTGGAACCAAGCGTCTGATTGGTAGACGCTTTGAGGATCCCCAAACTCAAAAAGAAATGAAGATGGTTCCATACAAGATAGTAAAGGCTCCAAATGGAGATGCTTGGGTTGAAGTCAACGGACAGCAGTACTCCCCAAGCCAAATTGGAGCCTTTGTTCTAACAAAGATGAAAGAGACTGCAGAGGCTTACCTTGGAAAGAGTGTCTCAAAAGCTGTGATTACAGTTCCAGCTTATTTCAATGATGCACAGAGACAAGCAACCAAGGATGCTGGCAGAATTGCAGGTCTAGATGTGCAGAGAATCATCAATGAGCCAACTGCTGCTGCACTTTCCTATGGTATGAACAACAAGGAAGGGCTCATAGCAGTGTTTGATCTTGGTGGGGGAACATTTGATGTATCCATTCTCGAGATATCCAATGGTGTTTTTGAGGTGAAAGCAACAAACGGCGACACATTCTTGGGAGGAGAGGATTTTGACAATGCGTTGTTGGACTTCTTGGTGAGTGAATTCAAGAGGACGGAGGGTATTGATTTGTCAAAGGATAGGCTTGCCTTGCAGAGGCTTAGGGAGGCAGCTGAGAAGGCTAAGATTGAACTTTCATCAACATCTCAAACTGAGATAAACCTTCCCTTCATCACAGCTGATTCTTCGGGTGCTAAACATCTGAACATCACATTGACTAGATCCAAATTTGAAAGTCTGGTCAATCACTTGATTGAGAGGACAAAGGCCCCGTGTAAGAACTGTTTGAAGGACGCTAATACATCCATTAAGGATGTGGATGAGGTTCTGCTTGTTGGAGGGATGACTCGTGTTCCCAAAGTGCAAGAGGTCGTTACAGAAATATTTGGAAAGAGCCCAAGCAAAGGAGTGAACCCTGATGAGGCTGTTGCTATGGGAGCTGCCATCCAAGGCGGTATCCTTCGTGGTGATGTTAAGGAGTTGCTTCTTTTGGATGTTACTCCTTTATCACTAGGTATTGAAACATTAGGTGGAATATTCACCAGGCTGATCAGTCGCAACACGACAATTCCAACCAAGAAGAGTCAGGTGTTTTCTACTGCAGCTGATAACCAAACCCAAGTGGGTATCAAGGTGCTACAAGGAGAGCGTGAGATGGCTTCTGACAACAAGATGTTGGGAGAGTTTGAACTCGTAGGAATTCCTCCAGCACCAAGAGGCCTGCCTCAGATCGAAGTCACCTTTGATATTGATGCCAACGGTATTGTCACTGTTTCTGCCAAGGACAAGGCCACCAACAAAGAGCAGCAGATTACTATCCGCTCATCTGGGGGCCTTAGTGACGAGGAGATTGAAAAGATGGTGAAGGAAGCGGAGCTGCATGCTCAGAAGGATCAGGAGAGGAAAGCATTGATTGACCTCAGAAATAGTGCAGATACAACCATCTACAGTATTGAGAAGAGCTTGAACGAGTATCGTGACAAGGTTCCAAGTGAAGTTGCCAAAGAAATTGAGGATGCGGTAGCAGATTTGAGGAAGGCAATGGGAGAGGACAATGCCGATGAAATCAAGGCCAAGCTTGAAGCTGCAAACAAAGCTGTTTCTAAGATTGGGGAACACATGTCAAAGGGTTCTGGTGGCGACTCGTCTTCTGGTGGATCACAGGGTGGAGACCAAGCTCCTGAGGCAGATTACGAAGAAGTGAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03018 ko04212 ko05152 map03018 map04212 map05152

Pfam Domains

Protein Families

HSP70

Protein Analysis

677

Amino Acids

72.78

Weight (kDa)

5.79

Isoelectric Point (pI)

40.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	55 - 648	5.3e-279	Hsp70 protein
MreB_Mbl	PF06723	184 - 421	5.2e-11	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002754)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37910 AT4G37910 AT5G09590
fragaria_vesca	FvH4_2g11170
malus_domestica	MD05G1049700.v1.1 MD10G1056800.v1.1 MD17G1201200.v1.1
prunus_persica	Prupe.3G077000_v2.0.a1 Prupe.8G075000_v2.0.a1 Prupe.8G075000_v2.0.a1
pyrus_communis	pycom05g04150 pycom10g04370 pycom17g20500 pycom17g20510
rosa_chinensis	RchiOBHm_Chr2g0137091 RchiOBHm_Chr5g0034081 RchiOBHm_Chr5g0057671 RchiOBHm_Chr6g0269491
rosa_laevigata	RLG00000019607
rosa_multiflora	Rmu_sc0001374.1_g000006 Rmu_sc0008218.1_g000001 Rmu_ssc0000291.1_g000035
rosa_roxburghii	Rroxscaffold_1G00027300 Rroxscaffold_2G00107660 Rroxscaffold_7G00198680
rosa_rugosa	Rorug02G0174600 Rorug02G0338100 Rorug02G0338200 Rorug06G0048600
rosa_samantha	Rh1CG066900 Rh1DG071800 Rh2BG227900 Rh2CG280100 Rh6AG168500 Rh6BG173900 Rh6CG168200 Rh6DG160200
rosa_wichuraiana	Rw2G031680 Rw6G014510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	157, 626
AccBSI	CCGCTC	2 cut(s)	24, 1641
AccI	GTMKAC	1 cut(s)	376
AciI	CCGC	7 cut(s)	9, 12, 22, 1272, 1639, 1691, 1838
AclWI	GGATC	6 cut(s)	66, 383, 396, 1035, 1719, 1984
AcoI	YGGCCR	2 cut(s)	3, 201
AcsI	RAATTY	4 cut(s)	238, 863, 1046, 1518
AdeI	CACNNNGTG	1 cut(s)	1985
AfaI	GTAC	3 cut(s)	184, 307, 482
AfiI	CCNNNNNNNGG	3 cut(s)	915, 1223, 1240
AflIII	ACRYGT	1 cut(s)	1938
AhdI	GACNNNNNGTC	1 cut(s)	41
AjiI	CACGTC	1 cut(s)	38
AjnI	CCWGG	1 cut(s)	1361
AjuI	GAANNNNNNNTTGG	2 cut(s)	285, 317
AloI	GAACNNNNNNTCC	2 cut(s)	492, 524
Alw21I	GWGCWC	1 cut(s)	253
Alw26I	GTCTC	4 cut(s)	521, 562, 598, 1983
AlwI	GGATC	6 cut(s)	66, 383, 396, 1035, 1719, 1984
Ama87I	CYCGRG	1 cut(s)	761
AoxI	GGCC	7 cut(s)	3, 201, 1086, 1537, 1608, 1651, 1890
ApeKI	GCWGC	8 cut(s)	674, 677, 929, 1259, 1421, 1624, 1696, 1904
ApoI	RAATTY	4 cut(s)	238, 863, 1046, 1518
AspLEI	GCGC	3 cut(s)	33, 65, 86
AspS9I	GGNCC	2 cut(s)	1087, 1651
AsuHPI	GGTGA	6 cut(s)	799, 868, 1298, 1351, 1549, 1693
AvaI	CYCGRG	1 cut(s)	761
AxyI	CCTNAGG	1 cut(s)	2001
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	388
BanII	GRGCYC	3 cut(s)	253, 714, 1217
BauI	CACGAG	1 cut(s)	1167
BbsI	GAAGAC	1 cut(s)	1959
Bbv12I	GWGCWC	1 cut(s)	253
BbvI	GCAGC	8 cut(s)	661, 664, 941, 1246, 1433, 1636, 1683, 1891
BccI	CCATC	8 cut(s)	108, 202, 271, 409, 1271, 1468, 1672, 1769
BceAI	ACGGC	2 cut(s)	188, 817
BciT130I	CCWGG	1 cut(s)	1363
BciVI	GTATCC	2 cut(s)	763, 1286
BclI	TGATCA	1 cut(s)	1368
BcoDI	GTCTC	4 cut(s)	521, 562, 598, 1983
BfaI	CTAG	3 cut(s)	641, 1038, 1331
BfmI	CTRYAG	3 cut(s)	531, 1419, 1765
BfuAI	ACCTGC	2 cut(s)	157, 626
BfuI	GTATCC	2 cut(s)	763, 1286
BisI	GCNGC	9 cut(s)	12, 675, 678, 930, 1260, 1422, 1625, 1697, 1905
BlsI	GCNGC	9 cut(s)	13, 676, 679, 931, 1261, 1423, 1626, 1698, 1906
BmcAI	AGTACT	1 cut(s)	482
Bme1390I	CCNGG	1 cut(s)	1363
BmeRI	GACNNNNNGTC	1 cut(s)	41
BmeT110I	CYCGRG	1 cut(s)	761
BmgBI	CACGTC	1 cut(s)	38
BmgT120I	GGNCC	2 cut(s)	1087, 1651
BmiI	GGNNCC	9 cut(s)	54, 358, 390, 420, 441, 502, 1089, 1652, 1806
BmrFI	CCNGG	1 cut(s)	1363
BmsI	GCATC	8 cut(s)	18, 56, 95, 442, 586, 610, 1561, 1825
BpiI	GAAGAC	1 cut(s)	1959
BpmI	CTGGAG	2 cut(s)	294, 1509
Bpu10I	CCTNAGC	1 cut(s)	920
Bsa29I	ATCGAT	1 cut(s)	70
BsaBI	GATNNNNATC	1 cut(s)	1634
BsaI	GGTCTC	1 cut(s)	1983
BsaJI	CCNNGG	4 cut(s)	544, 615, 1267, 1599
BsaXI	ACNNNNNCTCC	2 cut(s)	492, 522
Bsc4I	CCNNNNNNNGG	3 cut(s)	915, 1223, 1240
Bse21I	CCTNAGG	1 cut(s)	2001
Bse3DI	GCAATG	1 cut(s)	1866
Bse8I	GATNNNNATC	1 cut(s)	1634
BseBI	CCWGG	1 cut(s)	1363
BseCI	ATCGAT	1 cut(s)	70
BseDI	CCNNGG	4 cut(s)	544, 615, 1267, 1599
BseGI	GGATG	9 cut(s)	95, 625, 1121, 1138, 1144, 1167, 1263, 1318, 1840
BseJI	GATNNNNATC	1 cut(s)	1634
BseLI	CCNNNNNNNGG	3 cut(s)	915, 1223, 1240
BseMI	GCAATG	1 cut(s)	1866
BseMII	CTCAG	6 cut(s)	924, 963, 1559, 1718, 1753, 1992
BseRI	GAGGAG	3 cut(s)	45, 834, 1679
BseXI	GCAGC	8 cut(s)	661, 664, 941, 1246, 1433, 1636, 1683, 1891
BsgI	GTGCAG	3 cut(s)	663, 668, 1770
BshFI	GGCC	7 cut(s)	5, 203, 1088, 1539, 1610, 1653, 1892
BshVI	ATCGAT	1 cut(s)	70
BsiHKAI	GWGCWC	1 cut(s)	253
BsiHKCI	CYCGRG	1 cut(s)	761
BslI	CCNNNNNNNGG	3 cut(s)	915, 1223, 1240
BsmAI	GTCTC	4 cut(s)	521, 562, 598, 1983
BsmI	GAATGC	1 cut(s)	275
BsnI	GGCC	7 cut(s)	5, 203, 1088, 1539, 1610, 1653, 1892
Bso31I	GGTCTC	1 cut(s)	1983
BsoBI	CYCGRG	1 cut(s)	761
Bsp1286I	GDGCHC	3 cut(s)	253, 714, 1217
Bsp143I	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
BspACI	CCGC	7 cut(s)	9, 12, 22, 1272, 1639, 1691, 1838
BspANI	GGCC	7 cut(s)	5, 203, 1088, 1539, 1610, 1653, 1892
BspCNI	CTCAG	6 cut(s)	925, 964, 1558, 1717, 1752, 1993
BspDI	ATCGAT	1 cut(s)	70
BspLI	GGNNCC	9 cut(s)	54, 358, 390, 420, 441, 502, 1089, 1652, 1806
BspMAI	CTGCAG	2 cut(s)	535, 1423
BspMI	ACCTGC	2 cut(s)	157, 626
BspPI	GGATC	6 cut(s)	66, 383, 396, 1035, 1719, 1984
BspQI	GCTCTTC	1 cut(s)	1772
BspTNI	GGTCTC	1 cut(s)	1983
BsrBI	CCGCTC	2 cut(s)	24, 1641
BsrDI	GCAATG	1 cut(s)	1866
BssECI	CCNNGG	4 cut(s)	544, 615, 1267, 1599
BssMI	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
BssSI	CACGAG	1 cut(s)	1167
BssT1I	CCWWGG	4 cut(s)	544, 615, 1267, 1599
Bst2BI	CACGAG	1 cut(s)	1167
Bst2UI	CCWGG	1 cut(s)	1363
Bst4CI	ACNGT	6 cut(s)	349, 577, 1103, 1580, 1591, 1769
Bst6I	CTCTTC	2 cut(s)	1394, 1772
BstC8I	GCNNGC	5 cut(s)	121, 625, 906, 1541, 1701
BstENI	CCTNNNNNAGG	1 cut(s)	913
BstF5I	GGATG	9 cut(s)	95, 625, 1121, 1138, 1144, 1167, 1263, 1318, 1840
BstHHI	GCGC	3 cut(s)	33, 65, 86
BstKTI	GATC	8 cut(s)	74, 391, 730, 1043, 1371, 1551, 1714, 1979
BstMAI	GTCTC	4 cut(s)	521, 562, 598, 1983
BstMBI	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 30, 125, 910, 938, 1256, 1913, 2003
BstNI	CCWGG	1 cut(s)	1363
BstNSI	RCATGY	2 cut(s)	1703, 1942
BstSCI	CCNGG	1 cut(s)	1361
BstSFI	CTRYAG	3 cut(s)	531, 1419, 1765
BstV1I	GCAGC	8 cut(s)	661, 664, 941, 1246, 1433, 1636, 1683, 1891
BstV2I	GAAGAC	1 cut(s)	1959
BstX2I	RGATCY	2 cut(s)	388, 1040
BstYI	RGATCY	2 cut(s)	388, 1040
Bsu15I	ATCGAT	1 cut(s)	70
Bsu36I	CCTNAGG	1 cut(s)	2001
BsuI	GTATCC	2 cut(s)	763, 1286
BsuRI	GGCC	7 cut(s)	5, 203, 1088, 1539, 1610, 1653, 1892
BsuTUI	ATCGAT	1 cut(s)	70
BtrI	CACGTC	1 cut(s)	38
BtsCI	GGATG	9 cut(s)	95, 625, 1121, 1138, 1144, 1167, 1263, 1318, 1840
BtsI	GCAGTG	1 cut(s)	726
BtsIMutI	CAGTG	2 cut(s)	726, 1587
BveI	ACCTGC	2 cut(s)	157, 626
Cac8I	GCNNGC	5 cut(s)	121, 625, 906, 1541, 1701
CfoI	GCGC	3 cut(s)	33, 65, 86
Cfr13I	GGNCC	2 cut(s)	1087, 1651
ClaI	ATCGAT	1 cut(s)	70
CseI	GACGC	3 cut(s)	353, 387, 1121
Csp6I	GTAC	3 cut(s)	183, 306, 481
CviAII	CATG	3 cut(s)	195, 1700, 1939
CviQI	GTAC	3 cut(s)	183, 306, 481
DpnI	GATC	8 cut(s)	73, 390, 729, 1042, 1370, 1550, 1713, 1978
DpnII	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
DraIII	CACNNNGTG	1 cut(s)	1985
DriI	GACNNNNNGTC	1 cut(s)	41
EaeI	YGGCCR	2 cut(s)	3, 201
Eam1104I	CTCTTC	2 cut(s)	1394, 1772
Eam1105I	GACNNNNNGTC	1 cut(s)	41
EarI	CTCTTC	2 cut(s)	1394, 1772
Ecl136II	GAGCTC	1 cut(s)	251
Eco130I	CCWWGG	4 cut(s)	544, 615, 1267, 1599
Eco147I	AGGCCT	1 cut(s)	1539
Eco24I	GRGCYC	3 cut(s)	253, 714, 1217
Eco31I	GGTCTC	1 cut(s)	1983
Eco32I	GATATC	1 cut(s)	768
Eco53kI	GAGCTC	1 cut(s)	251
Eco81I	CCTNAGG	1 cut(s)	2001
Eco88I	CYCGRG	1 cut(s)	761
EcoICRI	GAGCTC	1 cut(s)	251
EcoNI	CCTNNNNNAGG	1 cut(s)	913
EcoO109I	RGGNCCY	2 cut(s)	1087, 1651
EcoRI	GAATTC	3 cut(s)	238, 863, 1518
EcoRII	CCWGG	1 cut(s)	1361
EcoRV	GATATC	1 cut(s)	768
EcoT14I	CCWWGG	4 cut(s)	544, 615, 1267, 1599
EcoT38I	GRGCYC	3 cut(s)	253, 714, 1217
ErhI	CCWWGG	4 cut(s)	544, 615, 1267, 1599
FaeI	CATG	3 cut(s)	198, 1703, 1942
FaiI	YATR	8 cut(s)	196, 424, 690, 695, 716, 1253, 1701, 1940
FatI	CATG	3 cut(s)	194, 1699, 1938
FbaI	TGATCA	1 cut(s)	1368
FblI	GTMKAC	1 cut(s)	376
Fnu4HI	GCNGC	9 cut(s)	12, 675, 678, 930, 1260, 1422, 1625, 1697, 1905
FokI	GGATG	9 cut(s)	82, 632, 1108, 1145, 1151, 1174, 1250, 1325, 1847
FriOI	GRGCYC	3 cut(s)	253, 714, 1217
Fsp4HI	GCNGC	9 cut(s)	12, 675, 678, 930, 1260, 1422, 1625, 1697, 1905
FspBI	CTAG	3 cut(s)	641, 1038, 1331
GlaI	GCGC	3 cut(s)	32, 64, 85
GluI	GCNGC	9 cut(s)	12, 675, 678, 930, 1260, 1422, 1625, 1697, 1905
GsuI	CTGGAG	2 cut(s)	294, 1509
HaeIII	GGCC	7 cut(s)	5, 203, 1088, 1539, 1610, 1653, 1892
HgaI	GACGC	3 cut(s)	353, 387, 1121
HhaI	GCGC	3 cut(s)	33, 65, 86
Hin1II	CATG	3 cut(s)	198, 1703, 1942
Hin6I	GCGC	3 cut(s)	31, 63, 84
HinP1I	GCGC	3 cut(s)	31, 63, 84
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HindIII	AAGCTT	2 cut(s)	121, 1895
HinfI	GANTC	5 cut(s)	654, 1001, 1165, 1402, 1961
HphI	GGTGA	6 cut(s)	799, 868, 1298, 1351, 1549, 1693
Hpy166II	GTNNAC	3 cut(s)	377, 469, 1231
Hpy188I	TCNGA	7 cut(s)	244, 369, 1023, 1483, 1548, 1707, 1742
Hpy188III	TCNNGA	7 cut(s)	641, 761, 763, 868, 1715, 1796, 2000
Hpy8I	GTNNAC	3 cut(s)	377, 469, 1231
Hpy99I	CGWCG	1 cut(s)	39
HpyCH4III	ACNGT	6 cut(s)	349, 577, 1103, 1580, 1591, 1769
HpyCH4IV	ACGT	2 cut(s)	37, 319
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 30, 125, 910, 938, 1256, 1913, 2003
HpySE526I	ACGT	2 cut(s)	37, 319
Hsp92II	CATG	3 cut(s)	198, 1703, 1942
HspAI	GCGC	3 cut(s)	31, 63, 84
Ksp22I	TGATCA	1 cut(s)	1368
Kzo9I	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
LguI	GCTCTTC	1 cut(s)	1772
LmnI	GCTCC	8 cut(s)	24, 58, 248, 445, 500, 1256, 1693, 2002
Lsp1109I	GCAGC	8 cut(s)	661, 664, 941, 1246, 1433, 1636, 1683, 1891
LweI	GCATC	8 cut(s)	18, 56, 95, 442, 586, 610, 1561, 1825
MaeI	CTAG	3 cut(s)	641, 1038, 1331
MaeII	ACGT	2 cut(s)	37, 319
MaeIII	GTNAC	7 cut(s)	328, 1192, 1315, 1555, 1585, 1658, 1796
MalI	GATC	8 cut(s)	73, 390, 729, 1042, 1370, 1550, 1713, 1978
MbiI	CCGCTC	2 cut(s)	24, 1641
MboI	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
MboII	GAAGA	6 cut(s)	424, 996, 1411, 1789, 1959, 2027
MflI	RGATCY	2 cut(s)	388, 1040
MhlI	GDGCHC	3 cut(s)	253, 714, 1217
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	238, 495, 630, 863, 1046, 1386, 1518, 1827
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	1159, 1411, 1955
MmeI	TCCRAC	3 cut(s)	825, 1135, 1415
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	1128, 1293
MslI	CAYNNNNRTG	1 cut(s)	719
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	932, 998, 1424
MspR9I	CCNGG	1 cut(s)	1363
Mva1269I	GAATGC	1 cut(s)	275
MvaI	CCWGG	1 cut(s)	1363
MwoI	GCNNNNNNNGC	8 cut(s)	11, 30, 125, 910, 938, 1256, 1913, 2003
NdeII	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
NlaIII	CATG	3 cut(s)	198, 1703, 1942
NlaIV	GGNNCC	9 cut(s)	54, 358, 390, 420, 441, 502, 1089, 1652, 1806
NmuCI	GTSAC	4 cut(s)	1555, 1585, 1658, 1796
NspI	RCATGY	2 cut(s)	1703, 1942
PaeI	GCATGC	1 cut(s)	1703
PaeR7I	CTCGAG	1 cut(s)	761
PceI	AGGCCT	1 cut(s)	1539
PciI	ACATGT	1 cut(s)	1938
PciSI	GCTCTTC	1 cut(s)	1772
PctI	GAATGC	1 cut(s)	275
PfeI	GAWTC	2 cut(s)	654, 1001
PkrI	GCNGC	9 cut(s)	13, 676, 679, 931, 1261, 1423, 1626, 1698, 1906
PleI	GAGTC	3 cut(s)	1159, 1410, 1955
PpsI	GAGTC	3 cut(s)	1159, 1410, 1955
PscI	ACATGT	1 cut(s)	1938
Psp124BI	GAGCTC	1 cut(s)	253
Psp6I	CCWGG	1 cut(s)	1361
PspGI	CCWGG	1 cut(s)	1361
PspN4I	GGNNCC	9 cut(s)	54, 358, 390, 420, 441, 502, 1089, 1652, 1806
PspPI	GGNCC	2 cut(s)	1087, 1651
PstI	CTGCAG	2 cut(s)	535, 1423
PsuI	RGATCY	2 cut(s)	388, 1040
PvuII	CAGCTG	3 cut(s)	932, 998, 1424
RsaI	GTAC	3 cut(s)	184, 307, 482
RsaNI	GTAC	3 cut(s)	183, 306, 481
RseI	CAYNNNNRTG	1 cut(s)	719
SacI	GAGCTC	1 cut(s)	253
SapI	GCTCTTC	1 cut(s)	1772
SaqAI	TTAA	2 cut(s)	1128, 1293
SatI	GCNGC	9 cut(s)	12, 675, 678, 930, 1260, 1422, 1625, 1697, 1905
Sau3AI	GATC	8 cut(s)	71, 388, 727, 1040, 1368, 1548, 1711, 1976
Sau96I	GGNCC	2 cut(s)	1087, 1651
ScaI	AGTACT	1 cut(s)	482
SchI	GAGTC	3 cut(s)	1159, 1411, 1955
ScrFI	CCNGG	1 cut(s)	1363
SduI	GDGCHC	3 cut(s)	253, 714, 1217
SfaNI	GCATC	8 cut(s)	18, 56, 95, 442, 586, 610, 1561, 1825
SfcI	CTRYAG	3 cut(s)	531, 1419, 1765
Sfr274I	CTCGAG	1 cut(s)	761
SlaI	CTCGAG	1 cut(s)	761
SmiMI	CAYNNNNRTG	1 cut(s)	719
SmlI	CTYRAG	1 cut(s)	761
SmoI	CTYRAG	1 cut(s)	761
SphI	GCATGC	1 cut(s)	1703
Sse9I	AATT	8 cut(s)	238, 495, 630, 863, 1046, 1386, 1518, 1827
SseBI	AGGCCT	1 cut(s)	1539
SsiI	CCGC	7 cut(s)	9, 12, 22, 1272, 1639, 1691, 1838
SspI	AATATT	2 cut(s)	1203, 1356
SspMI	CTAG	3 cut(s)	641, 1038, 1331
SstI	GAGCTC	1 cut(s)	253
StuI	AGGCCT	1 cut(s)	1539
StyD4I	CCNGG	1 cut(s)	1361
StyI	CCWWGG	4 cut(s)	544, 615, 1267, 1599
TaaI	ACNGT	6 cut(s)	349, 577, 1103, 1580, 1591, 1769
TaiI	ACGT	2 cut(s)	40, 322
TaqI	TCGA	5 cut(s)	26, 70, 253, 762, 1551
TasI	AATT	8 cut(s)	238, 495, 630, 863, 1046, 1386, 1518, 1827
TatI	WGTACW	1 cut(s)	480
TauI	GCSGC	1 cut(s)	14
TfiI	GAWTC	2 cut(s)	654, 1001
Tru1I	TTAA	2 cut(s)	1128, 1293
Tru9I	TTAA	2 cut(s)	1128, 1293
TscAI	CASTG	2 cut(s)	726, 1594
TseFI	GTSAC	4 cut(s)	1555, 1585, 1658, 1796
TseI	GCWGC	8 cut(s)	674, 677, 929, 1259, 1421, 1624, 1696, 1904
Tsp45I	GTSAC	4 cut(s)	1555, 1585, 1658, 1796
TspDTI	ATGAA	6 cut(s)	425, 536, 710, 945, 979, 1896
TspGWI	ACGGA	2 cut(s)	486, 890
TspRI	CASTG	2 cut(s)	726, 1594
XagI	CCTNNNNNAGG	1 cut(s)	913
XapI	RAATTY	4 cut(s)	238, 863, 1046, 1518
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	2 cut(s)	1703, 1942
XcmI	CCANNNNNNNNNTGG	2 cut(s)	196, 1132
XhoI	CTCGAG	1 cut(s)	761
XmiI	GTMKAC	1 cut(s)	376
XspI	CTAG	3 cut(s)	641, 1038, 1331
ZrmI	AGTACT	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.