Rorug02G0338200
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002754
Physical Location & Seq
Reverse (-)
42027356 .. 42027757
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0338200.1

Sequence Viewer

Length: 402 bp
ATGGACGATATGAAGACCCTGGAGTCTCACGATCAGCTGTCTCGATTCTTGAGCTCCGGCCACTCCGACGAGAAATTCGTCACTGACATCGTCATCAGCTTCACCCTCGCCGGAAGGGACACGACCTCGGCGGCTTTGACTTGGTTCTTCTGGCTAATCTCAAACCATCCAAACATCGAAAGCAAAATTCTCAAGGTGATCAATGAGAAAACGGAGGCCGGAGTCGGATATGATGAAGTGAAGGACATGAGGCTGTACCCGCCGATTCCGACCGACAGCAAAGAGGTGGTAGATGGAGCAGATCTGGGGCAAGGACTGGCCGAGTTCCGACCCGAGAGGTGGTTGGAGTGTGGCGTGGCGACGGAGACGTGGTGGTTCGTGGTGAAGGACTCATTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko05152 map03018 map04212 map05152
Pfam Domains
Protein Families
HSP70

Protein Analysis

133

Amino Acids

15.12

Weight (kDa)

4.61

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 27 - 84 7.2e-07 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AciI CCGC 2 cut(s) 131, 260
AcoI YGGCCR 2 cut(s) 58, 318
AcsI RAATTY 2 cut(s) 74, 186
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 339
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 3 cut(s) 37, 54, 99
AluI AGCT 3 cut(s) 37, 54, 99
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 3 cut(s) 30, 45, 359
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 3 cut(s) 58, 216, 318
ApoI RAATTY 2 cut(s) 74, 186
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AsuHPI GGTGA 3 cut(s) 94, 208, 394
AvaI CYCGRG 1 cut(s) 332
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 56
BccI CCATC 2 cut(s) 174, 287
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 198
BcoDI GTCTC 3 cut(s) 30, 45, 359
BfaI CTAG 1 cut(s) 400
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 20
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 70, 176
BsaJI CCNNGG 2 cut(s) 18, 126
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 18, 126
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 3 cut(s) 60, 218, 320
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 3 cut(s) 57, 111, 219
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 339
BsmAI GTCTC 3 cut(s) 30, 45, 359
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 60, 218, 320
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 3 cut(s) 31, 198, 301
BspACI CCGC 2 cut(s) 131, 260
BspANI GGCC 3 cut(s) 60, 218, 320
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 18, 126
BssMI GATC 3 cut(s) 31, 198, 301
Bst2UI CCWGG 1 cut(s) 20
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 3 cut(s) 34, 201, 304
BstMAI GTCTC 3 cut(s) 30, 45, 359
BstMBI GATC 3 cut(s) 31, 198, 301
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 3 cut(s) 60, 218, 320
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 81
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 247
CviJI RGCY 9 cut(s) 37, 54, 60, 99, 134, 154, 218, 253, 320
CviKI_1 RGCY 9 cut(s) 37, 54, 60, 99, 134, 154, 218, 253, 320
CviQI GTAC 1 cut(s) 256
DpnI GATC 3 cut(s) 33, 200, 303
DpnII GATC 3 cut(s) 31, 198, 301
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 2 cut(s) 58, 318
Ecl136II GAGCTC 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 56
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 1 cut(s) 250
FaiI YATR 3 cut(s) 11, 231, 248
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 246
FauI CCCGC 1 cut(s) 267
FbaI TGATCA 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 400
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 3 cut(s) 60, 218, 320
HapII CCGG 3 cut(s) 57, 111, 219
Hin1II CATG 1 cut(s) 250
HinfI GANTC 5 cut(s) 23, 45, 222, 265, 389
HpaII CCGG 3 cut(s) 57, 111, 219
HphI GGTGA 3 cut(s) 94, 208, 394
Hpy188I TCNGA 4 cut(s) 67, 227, 270, 329
Hpy188III TCNNGA 3 cut(s) 29, 42, 49
Hpy99I CGWCG 2 cut(s) 71, 364
HpyAV CCTTC 3 cut(s) 108, 235, 379
HpyCH4IV ACGT 1 cut(s) 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 3 cut(s) 31, 198, 301
LmnI GCTCC 2 cut(s) 59, 296
LpnPI CCDG 8 cut(s) 5, 32, 70, 124, 136, 232, 290, 302
MaeI CTAG 1 cut(s) 400
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 79
MalI GATC 3 cut(s) 33, 200, 303
MboI GATC 3 cut(s) 31, 198, 301
MboII GAAGA 2 cut(s) 25, 139
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 2 cut(s) 74, 186
MlyI GAGTC 3 cut(s) 32, 231, 383
MmeI TCCRAC 5 cut(s) 90, 205, 293, 324, 352
MnlI CCTC 6 cut(s) 116, 136, 208, 243, 277, 330
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 3 cut(s) 57, 111, 219
MspR9I CCNGG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 259
NdeII GATC 3 cut(s) 31, 198, 301
NlaIII CATG 1 cut(s) 250
NmeAIII GCCGAG 2 cut(s) 107, 346
NmuCI GTSAC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 2 cut(s) 45, 265
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 3 cut(s) 31, 230, 383
PpsI GAGTC 3 cut(s) 31, 230, 383
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SacI GAGCTC 1 cut(s) 56
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 3 cut(s) 31, 198, 301
SchI GAGTC 3 cut(s) 32, 231, 383
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 1 cut(s) 56
SetI ASST 8 cut(s) 39, 56, 101, 128, 198, 288, 341, 371
SmlI CTYRAG 2 cut(s) 49, 191
SmoI CTYRAG 2 cut(s) 49, 191
Sse9I AATT 2 cut(s) 74, 186
SsiI CCGC 2 cut(s) 131, 260
SspMI CTAG 1 cut(s) 400
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 18
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 43, 177
TaqII GACCGA 1 cut(s) 287
TasI AATT 2 cut(s) 74, 186
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 2 cut(s) 45, 265
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 26, 249
TspGWI ACGGA 2 cut(s) 227, 377
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 2 cut(s) 74, 186
XspI CTAG 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.