Rorug02G0338100
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002754
Physical Location & Seq
Reverse (-)
42006853 .. 42010601
3749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0338100.1

Sequence Viewer

Length: 1185 bp
ATGAGCTGCTGCGACTGTGCATCCAATCACTGCAATACAGAGACCCCAAAACCCAAGATTTTGGCCGTCATTCTTGATTTGGATGGGACCCTTTTGGACACAGAGCGGGCTACAAAGAGCGTCTTCAAGGAGTTTTTGGCCAAGTATGGCAAGACCTTGAATAGGGAAAGGGAAGAGAAGAGGGAGATTGGGAAGACACTCAAAGACTCGTCTACCGCCACTGTTGAGGACTATGATCTTCCATTGACTCCTGACCAGTTTATTGAAGAAATCATCCCCATGTATCAACAAAAGTGGAAGCACGCGAAAGCTCTTCCTGGTGCTACCCGTCTTATCAAACATCTCCATGACCATGGAGTACCTACTGCTCTTGCTTCAAATTCCTTGCGCGAATATATAAATGCAAAGATCTCTGTCCATCAAGGTTGGCAGGGATGGTTTTCAGTAATCCTTGGCAGTGACCAGGTTAAAGCGGGCAAGCCCTCTCCAGATTTATTTGAAGAGGCAGCAAGGCAGATGGATGTAGATGCAGCTCACTGCCTTGTGATCGAAGACTCGATGGTTGGCGTTAAAGCTGCCAATGCTGCCGGAATGGAGGTAGTGGCTGTTCCACCCCATGGTGAAGCTGCTGGTTGTTCTGCCCTTGCAAACACTGTGCTTCATTCACTTTTGGAGTTTAGACCTGAGCATTGGGGTCTTCCTCCTTTTGAAGATTGGGTAGATAATGCATTGCCAATTGAAGCAATTAATTTGAGTGGTCTTTATGTTAACGGGTTTGTCATTGAAGACACAGAGGATGGAAAATCTGCTCTGCCTGACCAAGTTTGGGGAGTTTTCTTTGGGTGGGCTGTGGTGGATATGCAGAAGAGCTACAGGGTAGTGGTTGGCATTGGAATGGATCACAATTCCTGCTGTCCTAAGAAAAAGACTCAAATACATCTAGTTAATGGAGGCAATTGCTGTATATCCAATCAGCAAGTGAAACTGCTGCTTGTTGGCTACATCCGCGGACTGAATAGCAAGGAAGTTTCATCTCTGGATGCAGAATCACTCGAGGAATGCAAGTCTATTGCAAGTGCTGCATTGGATCTGCCAATATTTAGTCAGCATGCTTATGTGCCTCTGTACCCAGATACTTTTTCTCTCGAAGACATGGTTGACACTGATGAGCTAGGACACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko05152 map03018 map04212 map05152
Pfam Domains
Protein Families
HSP70

Protein Analysis

394

Amino Acids

43.35

Weight (kDa)

5.08

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 22 - 197 2.6e-26 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 23 - 203 2.9e-28 Haloacid dehalogenase-like hydrolase
HAD PF12710 23 - 153 1.4e-08 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 158 - 203 4.6e-07 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 617
AccBSI CCGCTC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 212
AccII CGCG 3 cut(s) 305, 390, 1008
AciI CCGC 5 cut(s) 106, 216, 473, 1006, 1008
AclWI GGATC 2 cut(s) 906, 1095
AcoI YGGCCR 2 cut(s) 63, 138
AcsI RAATTY 1 cut(s) 379
AfaI GTAC 2 cut(s) 360, 1127
AfiI CCNNNNNNNGG 3 cut(s) 162, 617, 826
AgsI TTSAA 8 cut(s) 127, 160, 266, 378, 500, 710, 740, 785
AjnI CCWGG 2 cut(s) 316, 462
AluBI AGCT 7 cut(s) 6, 311, 533, 575, 626, 870, 1171
AluI AGCT 7 cut(s) 6, 311, 533, 575, 626, 870, 1171
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 2 cut(s) 906, 1095
Ama87I CYCGRG 1 cut(s) 1052
AoxI GGCC 2 cut(s) 63, 138
ApeKI GCWGC 9 cut(s) 6, 9, 506, 530, 575, 584, 626, 988, 1079
ApoI RAATTY 1 cut(s) 379
AseI ATTAAT 1 cut(s) 747
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 632
AvaI CYCGRG 1 cut(s) 1052
AvaII GGWCC 1 cut(s) 87
BalI TGGCCA 1 cut(s) 140
BbsI GAAGAC 6 cut(s) 115, 200, 558, 689, 792, 1155
BbvI GCAGC 7 cut(s) 518, 542, 562, 571, 613, 975, 1066
BccI CCATC 6 cut(s) 77, 426, 429, 511, 553, 791
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 2 cut(s) 318, 464
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 2 cut(s) 941, 1172
BfmI CTRYAG 1 cut(s) 871
BglII AGATCT 1 cut(s) 408
BisI GCNGC 9 cut(s) 7, 10, 507, 531, 576, 585, 627, 989, 1080
BlsI GCNGC 9 cut(s) 8, 11, 508, 532, 577, 586, 628, 990, 1081
Bme1390I CCNGG 2 cut(s) 318, 464
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 1052
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 88, 89
BmrFI CCNGG 2 cut(s) 318, 464
BmsI GCATC 3 cut(s) 29, 517, 1030
BpiI GAAGAC 6 cut(s) 115, 200, 558, 689, 792, 1155
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 4 cut(s) 352, 451, 616, 1006
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 617, 826
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 728
BseBI CCWGG 2 cut(s) 318, 464
BseDI CCNNGG 4 cut(s) 352, 451, 616, 1006
BseGI GGATG 8 cut(s) 20, 88, 273, 440, 526, 802, 1002, 1045
BseLI CCNNNNNNNGG 3 cut(s) 162, 617, 826
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 1 cut(s) 256
BseXI GCAGC 7 cut(s) 518, 542, 562, 571, 613, 975, 1066
Bsh1236I CGCG 3 cut(s) 305, 390, 1008
BshFI GGCC 2 cut(s) 65, 140
BsiHKCI CYCGRG 1 cut(s) 1052
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 3 cut(s) 162, 617, 826
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 1064
BsnI GGCC 2 cut(s) 65, 140
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 1052
Bsp143I GATC 5 cut(s) 235, 408, 546, 898, 1087
Bsp19I CCATGG 2 cut(s) 352, 616
BspACI CCGC 5 cut(s) 106, 216, 473, 1006, 1008
BspANI GGCC 2 cut(s) 65, 140
BspCNI CTCAG 1 cut(s) 676
BspFNI CGCG 3 cut(s) 305, 390, 1008
BspLI GGNNCC 2 cut(s) 88, 89
BspPI GGATC 2 cut(s) 906, 1095
BspQI GCTCTTC 2 cut(s) 318, 860
BspTNI GGTCTC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 4 cut(s) 352, 451, 616, 1006
BssMI GATC 5 cut(s) 235, 408, 546, 898, 1087
BssT1I CCWWGG 3 cut(s) 352, 451, 616
Bst2UI CCWGG 2 cut(s) 318, 464
Bst4CI ACNGT 3 cut(s) 17, 223, 655
Bst6I CTCTTC 5 cut(s) 168, 173, 318, 495, 860
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstC8I GCNNGC 5 cut(s) 108, 303, 475, 479, 1110
BstDEI CTNAG 2 cut(s) 684, 918
BstDSI CCRYGG 3 cut(s) 352, 616, 1006
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 8 cut(s) 20, 88, 273, 440, 526, 802, 1002, 1045
BstFNI CGCG 3 cut(s) 305, 390, 1008
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 5 cut(s) 238, 411, 549, 901, 1090
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 5 cut(s) 235, 408, 546, 898, 1087
BstMWI GCNNNNNNNGC 4 cut(s) 581, 584, 1005, 1079
BstNI CCWGG 2 cut(s) 318, 464
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 2 cut(s) 316, 462
BstSFI CTRYAG 1 cut(s) 871
BstUI CGCG 3 cut(s) 305, 390, 1008
BstV1I GCAGC 7 cut(s) 518, 542, 562, 571, 613, 975, 1066
BstV2I GAAGAC 6 cut(s) 115, 200, 558, 689, 792, 1155
BstX2I RGATCY 2 cut(s) 408, 1087
BstXI CCANNNNNNTGG 2 cut(s) 61, 353
BstYI RGATCY 2 cut(s) 408, 1087
BsuRI GGCC 2 cut(s) 65, 140
BtgI CCRYGG 3 cut(s) 352, 616, 1006
BtsCI GGATG 8 cut(s) 20, 88, 273, 440, 526, 802, 1002, 1045
BtsI GCAGTG 3 cut(s) 28, 463, 535
BtsIMutI CAGTG 6 cut(s) 28, 219, 463, 535, 651, 1161
Cac8I GCNNGC 5 cut(s) 108, 303, 475, 479, 1110
CfoI GCGC 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 87
Cfr42I CCGCGG 1 cut(s) 1009
CseI GACGC 1 cut(s) 109
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 2 cut(s) 359, 1126
CspCI CAANNNNNGTGG 2 cut(s) 275, 310
CviAII CATG 6 cut(s) 280, 347, 353, 617, 1109, 1153
CviQI GTAC 2 cut(s) 359, 1126
DdeI CTNAG 2 cut(s) 684, 918
DpnI GATC 5 cut(s) 237, 410, 548, 900, 1089
DpnII GATC 5 cut(s) 235, 408, 546, 898, 1087
EaeI YGGCCR 2 cut(s) 63, 138
Eam1104I CTCTTC 5 cut(s) 168, 173, 318, 495, 860
EarI CTCTTC 5 cut(s) 168, 173, 318, 495, 860
Eco130I CCWWGG 3 cut(s) 352, 451, 616
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 1052
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 316, 462
EcoT14I CCWWGG 3 cut(s) 352, 451, 616
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 3 cut(s) 352, 451, 616
FaeI CATG 6 cut(s) 283, 350, 356, 620, 1112, 1156
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 1 cut(s) 100
FatI CATG 6 cut(s) 279, 346, 352, 616, 1108, 1152
FauI CCCGC 2 cut(s) 99, 466
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 9 cut(s) 7, 10, 507, 531, 576, 585, 627, 989, 1080
FokI GGATG 8 cut(s) 7, 95, 260, 447, 533, 809, 989, 1052
Fsp4HI GCNGC 9 cut(s) 7, 10, 507, 531, 576, 585, 627, 989, 1080
FspBI CTAG 2 cut(s) 941, 1172
GlaI GCGC 1 cut(s) 389
GluI GCNGC 9 cut(s) 7, 10, 507, 531, 576, 585, 627, 989, 1080
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 2 cut(s) 65, 140
HapII CCGG 1 cut(s) 588
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 390
Hin1II CATG 6 cut(s) 283, 350, 356, 620, 1112, 1156
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 2 cut(s) 769, 1159
HindII GTYRAC 2 cut(s) 769, 1159
HinfI GANTC 5 cut(s) 206, 247, 554, 928, 1046
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 1 cut(s) 588
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 3 cut(s) 213, 769, 1159
Hpy188III TCNNGA 5 cut(s) 74, 251, 488, 1037, 1145
Hpy8I GTNNAC 3 cut(s) 213, 769, 1159
HpyCH4III ACNGT 3 cut(s) 17, 223, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 584, 1005, 1079
HpyF3I CTNAG 2 cut(s) 684, 918
Hsp92II CATG 6 cut(s) 283, 350, 356, 620, 1112, 1156
HspAI GCGC 1 cut(s) 388
KflI GGGWCCC 1 cut(s) 87
KspAI GTTAAC 1 cut(s) 769
KspI CCGCGG 1 cut(s) 1009
Kzo9I GATC 5 cut(s) 235, 408, 546, 898, 1087
LguI GCTCTTC 2 cut(s) 318, 860
Lsp1109I GCAGC 7 cut(s) 518, 542, 562, 571, 613, 975, 1066
LweI GCATC 3 cut(s) 29, 517, 1030
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 2 cut(s) 941, 1172
MaeIII GTNAC 1 cut(s) 458
MalI GATC 5 cut(s) 237, 410, 548, 900, 1089
MbiI CCGCTC 1 cut(s) 106
MboI GATC 5 cut(s) 235, 408, 546, 898, 1087
MfeI CAATTG 2 cut(s) 735, 955
MflI RGATCY 2 cut(s) 408, 1087
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 6 cut(s) 379, 735, 744, 748, 904, 955
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 4 cut(s) 200, 241, 548, 922
Mox20I TGGCCA 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 1 cut(s) 140
MseI TTAA 5 cut(s) 468, 570, 747, 768, 945
MslI CAYNNNNRTG 5 cut(s) 278, 345, 351, 893, 1113
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 1008
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 2 cut(s) 318, 464
MunI CAATTG 2 cut(s) 735, 955
Mva1269I GAATGC 1 cut(s) 1064
MvaI CCWGG 2 cut(s) 318, 464
MvnI CGCG 3 cut(s) 305, 390, 1008
MwoI GCNNNNNNNGC 4 cut(s) 581, 584, 1005, 1079
NcoI CCATGG 2 cut(s) 352, 616
NdeII GATC 5 cut(s) 235, 408, 546, 898, 1087
NlaIII CATG 6 cut(s) 283, 350, 356, 620, 1112, 1156
NlaIV GGNNCC 2 cut(s) 88, 89
NmuCI GTSAC 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 1112
PaeR7I CTCGAG 1 cut(s) 1052
PciSI GCTCTTC 2 cut(s) 318, 860
PctI GAATGC 1 cut(s) 1064
PfeI GAWTC 1 cut(s) 1046
PflMI CCANNNNNTGG 1 cut(s) 617
PkrI GCNGC 9 cut(s) 8, 11, 508, 532, 577, 586, 628, 990, 1081
PleI GAGTC 4 cut(s) 200, 241, 548, 922
PpsI GAGTC 4 cut(s) 200, 241, 548, 922
PpuMI RGGWCCY 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 316, 462
PspGI CCWGG 2 cut(s) 316, 462
PspN4I GGNNCC 2 cut(s) 88, 89
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 1052
PsrI GAACNNNNNNTAC 2 cut(s) 591, 623
PsuI RGATCY 2 cut(s) 408, 1087
RsaI GTAC 2 cut(s) 360, 1127
RsaNI GTAC 2 cut(s) 359, 1126
RseI CAYNNNNRTG 5 cut(s) 278, 345, 351, 893, 1113
SacII CCGCGG 1 cut(s) 1009
SapI GCTCTTC 2 cut(s) 318, 860
SaqAI TTAA 5 cut(s) 468, 570, 747, 768, 945
SatI GCNGC 9 cut(s) 7, 10, 507, 531, 576, 585, 627, 989, 1080
Sau3AI GATC 5 cut(s) 235, 408, 546, 898, 1087
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 4 cut(s) 200, 241, 548, 922
ScrFI CCNGG 2 cut(s) 318, 464
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 3 cut(s) 29, 517, 1030
SfcI CTRYAG 1 cut(s) 871
Sfr274I CTCGAG 1 cut(s) 1052
Sfr303I CCGCGG 1 cut(s) 1009
SgrBI CCGCGG 1 cut(s) 1009
SinI GGWCC 1 cut(s) 87
SlaI CTCGAG 1 cut(s) 1052
SmiMI CAYNNNNRTG 5 cut(s) 278, 345, 351, 893, 1113
SmlI CTYRAG 1 cut(s) 1052
SmoI CTYRAG 1 cut(s) 1052
SphI GCATGC 1 cut(s) 1112
Sse9I AATT 6 cut(s) 379, 735, 744, 748, 904, 955
SsiI CCGC 5 cut(s) 106, 216, 473, 1006, 1008
SspI AATATT 1 cut(s) 1098
SspMI CTAG 2 cut(s) 941, 1172
StyD4I CCNGG 2 cut(s) 316, 462
StyI CCWWGG 3 cut(s) 352, 451, 616
TaaI ACNGT 3 cut(s) 17, 223, 655
TaqI TCGA 4 cut(s) 549, 557, 1053, 1146
TasI AATT 6 cut(s) 379, 735, 744, 748, 904, 955
TfiI GAWTC 1 cut(s) 1046
Tru1I TTAA 5 cut(s) 468, 570, 747, 768, 945
Tru9I TTAA 5 cut(s) 468, 570, 747, 768, 945
TscAI CASTG 6 cut(s) 35, 226, 463, 542, 658, 1168
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 9 cut(s) 6, 9, 506, 530, 575, 584, 626, 988, 1079
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 2 cut(s) 650, 1020
TspRI CASTG 6 cut(s) 35, 226, 463, 542, 658, 1168
Van91I CCANNNNNTGG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 87
VspI ATTAAT 1 cut(s) 747
XagI CCTNNNNNAGG 1 cut(s) 160
XapI RAATTY 1 cut(s) 379
XceI RCATGY 1 cut(s) 1112
XhoI CTCGAG 1 cut(s) 1052
XmiI GTMKAC 1 cut(s) 212
XspI CTAG 2 cut(s) 941, 1172
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.