Rorug06G0048600
HSP70 Family

Heat shock 70 kDa protein, mitochondrial

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002754
Physical Location & Seq
Reverse (-)
6432434 .. 6433123
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0048600.1

Sequence Viewer

Length: 690 bp
ATGGAACCACAACAACAGCAACCAACAGAGGAGGGAGGAAACACCCAAGCAGCTGGAAGTAGTGCCAACATGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAATCCTCAAGGATCTTTACTATGTCAAGGGATTTAAGTGCCCAACTACAGAGCAGATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGGTATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAATCAGGCTGCTCCAGTGTCCATGAGAACTAGTTCTCTTGGTACTGGTAGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTGATCTCAACTTTGGTTCCACCTATTCTACTGGTAATGAAGGATTTCTTGATTTAAATTCTGTTTCATATTCTTCCTCACACTTCAACACTAATACTAGTACAACTCTTTTGGCACAACAGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTCAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTTTGGTAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko05152 map03018 map04212 map05152
Pfam Domains
Protein Families
HSP70

Protein Analysis

229

Amino Acids

25.36

Weight (kDa)

5.66

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 4.9e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 677
AccB7I CCANNNNNTGG 1 cut(s) 413
AclWI GGATC 7 cut(s) 141, 330, 378, 391, 411, 424, 459
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 3 cut(s) 91, 328, 571
AfiI CCNNNNNNNGG 2 cut(s) 413, 664
AgsI TTSAA 2 cut(s) 556, 640
AhlI ACTAGT 3 cut(s) 314, 443, 566
AjnI CCWGG 2 cut(s) 92, 629
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 3 cut(s) 53, 199, 410
AluI AGCT 3 cut(s) 53, 199, 410
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 661
AlwI GGATC 7 cut(s) 141, 330, 378, 391, 411, 424, 459
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 1 cut(s) 404
ApaLI GTGCAC 1 cut(s) 661
ApeKI GCWGC 2 cut(s) 50, 293
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 4 cut(s) 383, 415, 416, 448
Asp700I GAANNNNTTC 2 cut(s) 316, 513
AspS9I GGNCC 3 cut(s) 357, 404, 438
AsuHPI GGTGA 1 cut(s) 619
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 357, 438
BaeGI GKGCMC 2 cut(s) 164, 665
BamHI GGATCC 2 cut(s) 383, 416
BanII GRGCYC 1 cut(s) 262
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 2 cut(s) 62, 280
BccI CCATC 2 cut(s) 395, 452
BceAI ACGGC 2 cut(s) 72, 681
BciT130I CCWGG 2 cut(s) 94, 631
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 3 cut(s) 314, 443, 566
BfaI CTAG 3 cut(s) 315, 444, 567
BfmI CTRYAG 1 cut(s) 168
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 51, 294
BlsI GCNGC 2 cut(s) 52, 295
Bme1390I CCNGG 2 cut(s) 94, 631
Bme18I GGWCC 2 cut(s) 357, 438
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 357, 404, 438
BmiI GGNNCC 7 cut(s) 6, 245, 358, 385, 418, 439, 487
BmrFI CCNGG 2 cut(s) 94, 631
BpmI CTGGAG 3 cut(s) 93, 179, 282
BpuEI CTTGAG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 674
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 664
Bse1I ACTGG 6 cut(s) 205, 299, 334, 367, 376, 505
BseBI CCWGG 2 cut(s) 94, 631
BseGI GGATG 1 cut(s) 601
BseLI CCNNNNNNNGG 2 cut(s) 413, 664
BseNI ACTGG 6 cut(s) 205, 299, 334, 367, 376, 505
BseRI GAGGAG 3 cut(s) 44, 632, 635
BseSI GKGCMC 2 cut(s) 164, 665
BseXI GCAGC 2 cut(s) 62, 280
BshFI GGCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 261
BslI CCNNNNNNNGG 2 cut(s) 413, 664
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 1 cut(s) 406
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 164, 262, 665
Bsp143I GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
BspANI GGCC 1 cut(s) 406
BspLI GGNNCC 7 cut(s) 6, 245, 358, 385, 418, 439, 487
BspPI GGATC 7 cut(s) 141, 330, 378, 391, 411, 424, 459
BsrI ACTGG 6 cut(s) 205, 299, 334, 367, 376, 505
BssMI GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
BssNI GRCGYC 1 cut(s) 674
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 94, 631
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 1 cut(s) 674
BstC8I GCNNGC 1 cut(s) 408
BstEII GGTNACC 1 cut(s) 682
BstF5I GGATG 1 cut(s) 601
BstKTI GATC 9 cut(s) 136, 189, 338, 346, 386, 419, 467, 475, 627
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
BstNI CCWGG 2 cut(s) 94, 631
BstPI GGTNACC 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 92, 629
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 164, 665
BstV1I GCAGC 2 cut(s) 62, 280
BstX2I RGATCY 6 cut(s) 133, 186, 335, 383, 416, 464
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 6 cut(s) 133, 186, 335, 383, 416, 464
BsuRI GGCC 1 cut(s) 406
BtsCI GGATG 1 cut(s) 601
BtsIMutI CAGTG 2 cut(s) 306, 360
Cac8I GCNNGC 1 cut(s) 408
Cfr13I GGNCC 3 cut(s) 357, 404, 438
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 3 cut(s) 90, 327, 570
CviAII CATG 2 cut(s) 70, 307
CviJI RGCY 7 cut(s) 53, 87, 199, 260, 293, 406, 410
CviKI_1 RGCY 7 cut(s) 53, 87, 199, 260, 293, 406, 410
CviQI GTAC 3 cut(s) 90, 327, 570
DpnI GATC 9 cut(s) 135, 188, 337, 345, 385, 418, 466, 474, 626
DpnII GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
DraI TTTAAA 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 2 cut(s) 357, 438
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 682
EcoO65I GGTNACC 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 92, 629
EcoT38I GRGCYC 1 cut(s) 262
FaeI CATG 2 cut(s) 73, 310
FaiI YATR 6 cut(s) 71, 144, 210, 216, 308, 538
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FatI CATG 2 cut(s) 69, 306
Fnu4HI GCNGC 2 cut(s) 51, 294
FokI GGATG 1 cut(s) 588
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 2 cut(s) 51, 294
FspBI CTAG 3 cut(s) 315, 444, 567
GluI GCNGC 2 cut(s) 51, 294
GsuI CTGGAG 3 cut(s) 93, 179, 282
HaeIII GGCC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 2 cut(s) 73, 310
HinfI GANTC 3 cut(s) 100, 123, 178
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 3 cut(s) 360, 441, 663
Hpy188III TCNNGA 5 cut(s) 110, 182, 247, 263, 518
Hpy8I GTNNAC 3 cut(s) 360, 441, 663
HpyAV CCTTC 1 cut(s) 503
HpyCH4IV ACGT 2 cut(s) 252, 674
HpyCH4V TGCA 2 cut(s) 609, 663
HpySE526I ACGT 2 cut(s) 252, 674
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 2 cut(s) 73, 310
Kzo9I GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 2 cut(s) 62, 280
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 3 cut(s) 315, 444, 567
MaeII ACGT 2 cut(s) 252, 674
MaeIII GTNAC 1 cut(s) 682
MalI GATC 9 cut(s) 135, 188, 337, 345, 385, 418, 466, 474, 626
MboI GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
MboII GAAGA 2 cut(s) 534, 637
MflI RGATCY 6 cut(s) 133, 186, 335, 383, 416, 464
MhlI GDGCHC 3 cut(s) 164, 262, 665
MluCI AATT 4 cut(s) 349, 397, 430, 526
MroXI GAANNNNTTC 2 cut(s) 316, 513
MseI TTAA 2 cut(s) 156, 524
MspA1I CMGCKG 3 cut(s) 53, 199, 410
MspR9I CCNGG 2 cut(s) 94, 631
MvaI CCWGG 2 cut(s) 94, 631
NdeII GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
NlaIII CATG 2 cut(s) 73, 310
NlaIV GGNNCC 7 cut(s) 6, 245, 358, 385, 418, 439, 487
PaeR7I CTCGAG 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 671
PdmI GAANNNNTTC 2 cut(s) 316, 513
PfeI GAWTC 3 cut(s) 100, 123, 178
PflMI CCANNNNNTGG 1 cut(s) 413
PkrI GCNGC 2 cut(s) 52, 295
Psp6I CCWGG 2 cut(s) 92, 629
PspEI GGTNACC 1 cut(s) 682
PspGI CCWGG 2 cut(s) 92, 629
PspN4I GGNNCC 7 cut(s) 6, 245, 358, 385, 418, 439, 487
PspPI GGNCC 3 cut(s) 357, 404, 438
PsuI RGATCY 6 cut(s) 133, 186, 335, 383, 416, 464
PvuII CAGCTG 3 cut(s) 53, 199, 410
RsaI GTAC 3 cut(s) 91, 328, 571
RsaNI GTAC 3 cut(s) 90, 327, 570
SaqAI TTAA 2 cut(s) 156, 524
SatI GCNGC 2 cut(s) 51, 294
Sau3AI GATC 9 cut(s) 133, 186, 335, 343, 383, 416, 464, 472, 624
Sau96I GGNCC 3 cut(s) 357, 404, 438
ScrFI CCNGG 2 cut(s) 94, 631
SduI GDGCHC 3 cut(s) 164, 262, 665
SexAI ACCWGGT 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 357, 438
SlaI CTCGAG 1 cut(s) 261
SmiI ATTTAAAT 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 128, 261
SmoI CTYRAG 2 cut(s) 128, 261
SpeI ACTAGT 3 cut(s) 314, 443, 566
Sse9I AATT 4 cut(s) 349, 397, 430, 526
SspMI CTAG 3 cut(s) 315, 444, 567
StyD4I CCNGG 2 cut(s) 92, 629
SwaI ATTTAAAT 1 cut(s) 525
TaiI ACGT 2 cut(s) 255, 677
TaqI TCGA 2 cut(s) 262, 390
TasI AATT 4 cut(s) 349, 397, 430, 526
TatI WGTACW 1 cut(s) 569
TfiI GAWTC 3 cut(s) 100, 123, 178
Tru1I TTAA 2 cut(s) 156, 524
Tru9I TTAA 2 cut(s) 156, 524
TscAI CASTG 2 cut(s) 306, 367
TseI GCWGC 2 cut(s) 50, 293
TspDTI ATGAA 3 cut(s) 290, 522, 525
TspRI CASTG 2 cut(s) 306, 367
Van91I CCANNNNNTGG 1 cut(s) 413
VneI GTGCAC 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 357, 438
XapI RAATTY 1 cut(s) 526
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 316, 513
XspI CTAG 3 cut(s) 315, 444, 567
ZraI GACGTC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.