Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002754

Physical Location & Seq

Genomic Coordinates Forward (+)

31665806 .. 31669180

Gene Structure 3375 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00107660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGGCCACCGCAGCATTGCTCCGTTCACTATGCCGTCGTGATGTTGCAACCGCCTCCTTCTCTGCCTACCGCTCTATTCCCAAAGTTGTTGAGAATTCTGAGGGTGCTCGGACCACACCATCAGTAGTTGCCTTCAATCAGAAAGGAGAGCTACTAGTTGGTACTCCAGCAAAACGTCAAGCGGTCACCAACCCATCGAATACAGTTTATGCAGCCAAGCGACTGATTGCTAGGCGTTTTGATGATCCCCAAATACAAAAAGAAATGAAGATGGTTCCATATAAGATAGTCAAATCTCCAAACGGAGATTCATGGGTTGACATCAATGGGCAGCAGTACTCCCCAAGCCAAATTGGAGCCTTTGTTCTTACAAAGATGAAAGAAACTGCTGAGGCTTACCTAGGAAAGTCTGTGTCCAAAGCTGTGATTACAGTTCCAGCTTATTTTGATGATGCTCAGAGACAAGCGACAACTGATGCCGGAAGGATTGCAGGCCTCGAAGTACTCAGAATCATAAATGAGCCAACTGCTGCTGCACTATCCTACGGTATGAACAGCAAGGAAGGCCTAATAGCAGTATTTGATCTTGGTGGTGGAACATTTGATGTGTCCATTTTGGAGATATCAAATGGTGTGAAAGCAACCAATGGTGACACATTATTGGGAGGAGAGGATTTTGACAACGCTTTGCTGGACTACTTGGTGAGCGAATTCAAAAGGACTGATGGGATCGATCTTACGAAAGATAAGCTTGCCTTGCAGAGGCTTCGGGAGGCAGCTGAGAAGTCTAAGATTGAACTTTCATCAACATCCCAAACAGAGATAAACCTGCCCTTCATTACAGCTGATGCTTCAGGTGCTAAACATCTGAACATCACCCTGACCAGGTCAAAATTTGAAAGTCTGGTAAATCACTTGATTGAGAGGACAAAGGCTCCATGTCAGAATTGTTTGAAGGATGCCAACTTGACAATTAAGGATGTGGATGAGGTCCTTCTTGTTGGAGGAATGTCTCGTGTTCCCAAAGTGCAAGAGGTGGTGCAAGAGATATTCGGAAAAAGCGCAAGCAAAGGGGTCAATCCTGATGAGGCAGTTGCCTTGGGAGCAGCCATCCAAGGTGGTATACTCCGTGGTGATGTTAAGGAGTTGCTTCTTCTTGATGTCACTCCTCTATCACTAGGCATTGAGACAATGGGTGGTATCTTCACCAAGCTGATTGCCAGGAACACAACAATTCCAACAAAGAAGAGTCAGGTCTTTTCGACAGCAGCTGACAACCAAACCCAAGTTGGTGTCAGGGTGCTACAAGGTGAGCGTGAAATGGCTTGTGACAACAAGCCCTTGGGAGAGTTTGAGCTCCGAGGCATTCCTCCAGCACCAAGAGGCATGCCTCAGATTGAAGTCACATTTGATATTGATGCCAATGGCATAGTTACCGTTTCTGCCAAGGACAAAGCCACTGGTAAAGAACAACAGGTTACTATCAACAACACATCAGGTCGGCTCAGTGAAGAGGAAATTGAGAGGATGGTCAAGGAAGCAGAGATGCACGCTCAGAGGGATCAGGAAAAGAAAGCTCTCGTTGATCTCAGAAACAGTGCAGAGACCAGCATCTACAGCATTGAGAAGAGCTTAGACGAGTACCGTGACAAGGTTCCAAGTGAAACCGCTAAACAAATCGAGGATTCAGTTGCAGATTTGAGAAAGGCAATGGGAGAGGATAAGGTGGATGAACTTAAGTCCAAGCTTGATGCTGCAAACAGAGCTGTCTCCAAGATTGGGGAGCACATGTCTGGTGGTTCTGGAGGTAGCTCCTCATCTGGTTCACAGGGTGGAGACCAGACTCCTGAGGCAGATTTCTACGAAGAAGCGAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03018 ko04212 ko05152 map03018 map04212 map05152

Pfam Domains

Protein Families

HSP70

Protein Analysis

626

Amino Acids

67.63

Weight (kDa)

5.45

Isoelectric Point (pI)

40.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	27 - 597	6.1e-255	Hsp70 protein
MreB_Mbl	PF06723	136 - 370	1e-13	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002754)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37910 AT4G37910 AT5G09590
fragaria_vesca	FvH4_2g11170
malus_domestica	MD05G1049700.v1.1 MD10G1056800.v1.1 MD17G1201200.v1.1
prunus_persica	Prupe.3G077000_v2.0.a1 Prupe.8G075000_v2.0.a1 Prupe.8G075000_v2.0.a1
pyrus_communis	pycom05g04150 pycom10g04370 pycom17g20500 pycom17g20510
rosa_chinensis	RchiOBHm_Chr2g0137091 RchiOBHm_Chr5g0034081 RchiOBHm_Chr5g0057671 RchiOBHm_Chr6g0269491
rosa_laevigata	RLG00000019607
rosa_multiflora	Rmu_sc0001374.1_g000006 Rmu_sc0008218.1_g000001 Rmu_ssc0000291.1_g000035
rosa_roxburghii	Rroxscaffold_1G00027300 Rroxscaffold_2G00107660 Rroxscaffold_7G00198680
rosa_rugosa	Rorug02G0174600 Rorug02G0338100 Rorug02G0338200 Rorug06G0048600
rosa_samantha	Rh1CG066900 Rh1DG071800 Rh2BG227900 Rh2CG280100 Rh6AG168500 Rh6BG173900 Rh6CG168200 Rh6DG160200
rosa_wichuraiana	Rw2G031680 Rw6G014510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	837
AccBSI	CCGCTC	1 cut(s)	72
AccI	GTMKAC	1 cut(s)	1123
AciI	CCGC	5 cut(s)	9, 51, 70, 182, 1668
AclWI	GGATC	3 cut(s)	239, 737, 1569
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	94, 710, 893
AcuI	CTGAAG	1 cut(s)	837
AdeI	CACNNNGTG	1 cut(s)	1832
AfaI	GTAC	4 cut(s)	163, 338, 504, 1643
AfiI	CCNNNNNNNGG	4 cut(s)	762, 885, 1651, 1779
AflII	CTTAAG	1 cut(s)	1736
AflIII	ACRYGT	1 cut(s)	1788
AgsI	TTSAA	6 cut(s)	136, 715, 797, 899, 955, 1400
AhlI	ACTAGT	1 cut(s)	154
AjnI	CCWGG	2 cut(s)	884, 1220
AjuI	GAANNNNNNNTTGG	2 cut(s)	292, 324
AloI	GAACNNNNNNTCC	2 cut(s)	348, 380
Alw21I	GWGCWC	3 cut(s)	109, 1359, 1788
Alw26I	GTCTC	6 cut(s)	454, 1017, 1181, 1598, 1774, 1830
AlwI	GGATC	3 cut(s)	239, 737, 1569
AlwNI	CAGNNNCTG	1 cut(s)	1271
AoxI	GGCC	3 cut(s)	3, 493, 565
ApeKI	GCWGC	9 cut(s)	11, 212, 331, 530, 533, 776, 1106, 1268, 1754
ApoI	RAATTY	3 cut(s)	94, 710, 893
AspA2I	CCTAGG	1 cut(s)	400
AspLEI	GCGC	1 cut(s)	1064
AspS9I	GGNCC	2 cut(s)	111, 991
AsuHPI	GGTGA	7 cut(s)	178, 662, 715, 868, 1145, 1198, 1322
AvaII	GGWCC	2 cut(s)	111, 991
AvrII	CCTAGG	1 cut(s)	400
AxyI	CCTNAGG	1 cut(s)	1848
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	1359
BauI	CACGAG	1 cut(s)	1014
Bbv12I	GWGCWC	3 cut(s)	109, 1359, 1788
BbvCI	CCTCAGC	1 cut(s)	390
BbvI	GCAGC	9 cut(s)	23, 224, 343, 517, 520, 788, 1118, 1280, 1741
BccI	CCATC	6 cut(s)	127, 202, 265, 719, 1118, 1522
BceAI	ACGGC	1 cut(s)	18
BcgI	CGANNNNNNTGC	2 cut(s)	749, 783
BciT130I	CCWGG	2 cut(s)	886, 1222
BcoDI	GTCTC	6 cut(s)	454, 1017, 1181, 1598, 1774, 1830
BcuI	ACTAGT	1 cut(s)	154
BfaI	CTAG	4 cut(s)	155, 231, 401, 1178
BfmI	CTRYAG	1 cut(s)	1615
BfrI	CTTAAG	1 cut(s)	1736
BfuAI	ACCTGC	1 cut(s)	837
BisI	GCNGC	9 cut(s)	12, 213, 332, 531, 534, 777, 1107, 1269, 1755
BlnI	CCTAGG	1 cut(s)	400
BlsI	GCNGC	9 cut(s)	13, 214, 333, 532, 535, 778, 1108, 1270, 1756
BmcAI	AGTACT	2 cut(s)	338, 504
Bme1390I	CCNGG	2 cut(s)	886, 1222
Bme18I	GGWCC	2 cut(s)	111, 991
BmgT120I	GGNCC	2 cut(s)	111, 991
BmiI	GGNNCC	4 cut(s)	276, 358, 936, 1656
BmrFI	CCNGG	2 cut(s)	886, 1222
BmsI	GCATC	8 cut(s)	442, 466, 838, 949, 1408, 1536, 1620, 1741
BpmI	CTGGAG	3 cut(s)	150, 1356, 1824
Bpu10I	CCTNAGC	1 cut(s)	390
Bsa29I	ATCGAT	1 cut(s)	732
BsaI	GGTCTC	2 cut(s)	1598, 1830
BsaJI	CCNNGG	7 cut(s)	400, 1098, 1114, 1129, 1341, 1360, 1446
BsaXI	ACNNNNNCTCC	6 cut(s)	348, 378, 919, 949, 1775, 1805
Bsc4I	CCNNNNNNNGG	4 cut(s)	762, 885, 1651, 1779
Bse1I	ACTGG	1 cut(s)	1465
Bse21I	CCTNAGG	1 cut(s)	1848
Bse3DI	GCAATG	2 cut(s)	14, 1716
BseBI	CCWGG	2 cut(s)	886, 1222
BseCI	ATCGAT	1 cut(s)	732
BseDI	CCNNGG	7 cut(s)	400, 1098, 1114, 1129, 1341, 1360, 1446
BseGI	GGATG	7 cut(s)	809, 964, 985, 991, 1110, 1533, 1735
BseLI	CCNNNNNNNGG	4 cut(s)	762, 885, 1651, 1779
BseMI	GCAATG	2 cut(s)	14, 1716
BseNI	ACTGG	1 cut(s)	1465
BseRI	GAGGAG	3 cut(s)	681, 1158, 1804
BseXI	GCAGC	9 cut(s)	23, 224, 343, 517, 520, 788, 1118, 1280, 1741
BsgI	GTGCAG	2 cut(s)	519, 1620
BshFI	GGCC	3 cut(s)	5, 495, 567
BshVI	ATCGAT	1 cut(s)	732
BsiHKAI	GWGCWC	3 cut(s)	109, 1359, 1788
BsiSI	CCGG	1 cut(s)	480
BslI	CCNNNNNNNGG	4 cut(s)	762, 885, 1651, 1779
BsmAI	GTCTC	6 cut(s)	454, 1017, 1181, 1598, 1774, 1830
BsmI	GAATGC	1 cut(s)	1365
BsnI	GGCC	3 cut(s)	5, 495, 567
Bso31I	GGTCTC	2 cut(s)	1598, 1830
Bsp1286I	GDGCHC	3 cut(s)	109, 1359, 1788
Bsp143I	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
BspACI	CCGC	5 cut(s)	9, 51, 70, 182, 1668
BspANI	GGCC	3 cut(s)	5, 495, 567
BspDI	ATCGAT	1 cut(s)	732
BspLI	GGNNCC	4 cut(s)	276, 358, 936, 1656
BspMI	ACCTGC	1 cut(s)	837
BspPI	GGATC	3 cut(s)	239, 737, 1569
BspQI	GCTCTTC	1 cut(s)	1622
BspTI	CTTAAG	1 cut(s)	1736
BspTNI	GGTCTC	2 cut(s)	1598, 1830
BsrBI	CCGCTC	1 cut(s)	72
BsrDI	GCAATG	2 cut(s)	14, 1716
BsrI	ACTGG	1 cut(s)	1465
BssECI	CCNNGG	7 cut(s)	400, 1098, 1114, 1129, 1341, 1360, 1446
BssMI	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
BssNAI	GTATAC	1 cut(s)	1124
BssSI	CACGAG	1 cut(s)	1014
BssT1I	CCWWGG	5 cut(s)	400, 1098, 1114, 1341, 1446
Bst1107I	GTATAC	1 cut(s)	1124
Bst2BI	CACGAG	1 cut(s)	1014
Bst2UI	CCWGG	2 cut(s)	886, 1222
Bst4CI	ACNGT	6 cut(s)	205, 433, 548, 1438, 1598, 1646
Bst6I	CTCTTC	3 cut(s)	1241, 1506, 1622
BstAFI	CTTAAG	1 cut(s)	1736
BstC8I	GCNNGC	5 cut(s)	493, 753, 1066, 1388, 1551
BstDSI	CCRYGG	1 cut(s)	1129
BstEII	GGTNACC	1 cut(s)	184
BstENI	CCTNNNNNAGG	1 cut(s)	760
BstF5I	GGATG	7 cut(s)	809, 964, 985, 991, 1110, 1533, 1735
BstHHI	GCGC	1 cut(s)	1064
BstKTI	GATC	6 cut(s)	247, 586, 732, 736, 1564, 1588
BstMAI	GTCTC	6 cut(s)	454, 1017, 1181, 1598, 1774, 1830
BstMBI	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 564, 757, 857, 1103, 1617, 1763
BstNI	CCWGG	2 cut(s)	886, 1222
BstNSI	RCATGY	2 cut(s)	1390, 1792
BstPI	GGTNACC	1 cut(s)	184
BstSCI	CCNGG	2 cut(s)	884, 1220
BstSFI	CTRYAG	1 cut(s)	1615
BstV1I	GCAGC	9 cut(s)	23, 224, 343, 517, 520, 788, 1118, 1280, 1741
BstZ17I	GTATAC	1 cut(s)	1124
Bsu15I	ATCGAT	1 cut(s)	732
Bsu36I	CCTNAGG	1 cut(s)	1848
BsuRI	GGCC	3 cut(s)	5, 495, 567
BsuTUI	ATCGAT	1 cut(s)	732
BtgI	CCRYGG	1 cut(s)	1129
BtsCI	GGATG	7 cut(s)	809, 964, 985, 991, 1110, 1533, 1735
BtsIMutI	CAGTG	3 cut(s)	1458, 1513, 1603
BveI	ACCTGC	1 cut(s)	837
Cac8I	GCNNGC	5 cut(s)	493, 753, 1066, 1388, 1551
CaiI	CAGNNNCTG	1 cut(s)	1271
CfoI	GCGC	1 cut(s)	1064
Cfr13I	GGNCC	2 cut(s)	111, 991
ClaI	ATCGAT	1 cut(s)	732
CsiI	ACCWGGT	1 cut(s)	884
Csp6I	GTAC	4 cut(s)	162, 337, 503, 1642
CviAII	CATG	4 cut(s)	312, 939, 1387, 1789
CviQI	GTAC	4 cut(s)	162, 337, 503, 1642
DpnI	GATC	6 cut(s)	246, 585, 731, 735, 1563, 1587
DpnII	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
DraIII	CACNNNGTG	1 cut(s)	1832
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	1241, 1506, 1622
EarI	CTCTTC	3 cut(s)	1241, 1506, 1622
Ecl136II	GAGCTC	1 cut(s)	1357
Eco130I	CCWWGG	5 cut(s)	400, 1098, 1114, 1341, 1446
Eco147I	AGGCCT	2 cut(s)	495, 567
Eco24I	GRGCYC	1 cut(s)	1359
Eco31I	GGTCTC	2 cut(s)	1598, 1830
Eco32I	GATATC	1 cut(s)	624
Eco47I	GGWCC	2 cut(s)	111, 991
Eco53kI	GAGCTC	1 cut(s)	1357
Eco57I	CTGAAG	1 cut(s)	837
Eco81I	CCTNAGG	1 cut(s)	1848
Eco91I	GGTNACC	1 cut(s)	184
EcoICRI	GAGCTC	1 cut(s)	1357
EcoNI	CCTNNNNNAGG	1 cut(s)	760
EcoO109I	RGGNCCY	1 cut(s)	991
EcoO65I	GGTNACC	1 cut(s)	184
EcoRI	GAATTC	2 cut(s)	94, 710
EcoRII	CCWGG	2 cut(s)	884, 1220
EcoRV	GATATC	1 cut(s)	624
EcoT14I	CCWWGG	5 cut(s)	400, 1098, 1114, 1341, 1446
EcoT38I	GRGCYC	1 cut(s)	1359
ErhI	CCWWGG	5 cut(s)	400, 1098, 1114, 1341, 1446
FaeI	CATG	4 cut(s)	315, 942, 1390, 1792
FalI	AAGNNNNNCTT	4 cut(s)	735, 767, 740, 772
FatI	CATG	4 cut(s)	311, 938, 1386, 1788
FblI	GTMKAC	1 cut(s)	1123
Fnu4HI	GCNGC	9 cut(s)	12, 213, 332, 531, 534, 777, 1107, 1269, 1755
FokI	GGATG	7 cut(s)	796, 971, 992, 998, 1097, 1540, 1742
FriOI	GRGCYC	1 cut(s)	1359
Fsp4HI	GCNGC	9 cut(s)	12, 213, 332, 531, 534, 777, 1107, 1269, 1755
FspBI	CTAG	4 cut(s)	155, 231, 401, 1178
GlaI	GCGC	1 cut(s)	1063
GluI	GCNGC	9 cut(s)	12, 213, 332, 531, 534, 777, 1107, 1269, 1755
GsuI	CTGGAG	3 cut(s)	150, 1356, 1824
HaeIII	GGCC	3 cut(s)	5, 495, 567
HapII	CCGG	1 cut(s)	480
HhaI	GCGC	1 cut(s)	1064
Hin1II	CATG	4 cut(s)	315, 942, 1390, 1792
Hin6I	GCGC	1 cut(s)	1062
HinP1I	GCGC	1 cut(s)	1062
HincII	GTYRAC	1 cut(s)	319
HindII	GTYRAC	1 cut(s)	319
HindIII	AAGCTT	2 cut(s)	749, 1745
HinfI	GANTC	5 cut(s)	308, 510, 1249, 1685, 1843
HpaII	CCGG	1 cut(s)	480
HphI	GGTGA	7 cut(s)	178, 662, 715, 868, 1145, 1198, 1322
Hpy166II	GTNNAC	4 cut(s)	26, 319, 1124, 1826
Hpy188III	TCNNGA	7 cut(s)	38, 770, 1082, 1157, 1565, 1803, 1847
Hpy8I	GTNNAC	4 cut(s)	26, 319, 1124, 1826
Hpy99I	CGWCG	1 cut(s)	39
HpyAV	CCTTC	7 cut(s)	67, 142, 477, 557, 844, 949, 1004
HpyCH4III	ACNGT	6 cut(s)	205, 433, 548, 1438, 1598, 1646
HpyCH4IV	ACGT	1 cut(s)	175
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 564, 757, 857, 1103, 1617, 1763
HpySE526I	ACGT	1 cut(s)	175
Hsp92II	CATG	4 cut(s)	315, 942, 1390, 1792
HspAI	GCGC	1 cut(s)	1062
Kzo9I	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
LguI	GCTCTTC	1 cut(s)	1622
LmnI	GCTCC	7 cut(s)	24, 356, 940, 1103, 1362, 1783, 1817
Lsp1109I	GCAGC	9 cut(s)	23, 224, 343, 517, 520, 788, 1118, 1280, 1741
LweI	GCATC	8 cut(s)	442, 466, 838, 949, 1408, 1536, 1620, 1741
MabI	ACCWGGT	1 cut(s)	884
MaeI	CTAG	4 cut(s)	155, 231, 401, 1178
MaeII	ACGT	1 cut(s)	175
MaeIII	GTNAC	8 cut(s)	184, 650, 1162, 1328, 1402, 1432, 1477, 1646
MalI	GATC	6 cut(s)	246, 585, 731, 735, 1563, 1587
MbiI	CCGCTC	1 cut(s)	72
MboI	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
MboII	GAAGA	7 cut(s)	280, 1145, 1195, 1258, 1523, 1639, 1877
MhlI	GDGCHC	3 cut(s)	109, 1359, 1788
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	94, 351, 710, 893, 946, 972, 1233, 1518
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	1258, 1837
MmeI	TCCRAC	2 cut(s)	982, 1262
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	975, 1140, 1737
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	779, 845, 1271
MspCI	CTTAAG	1 cut(s)	1736
MspI	CCGG	1 cut(s)	480
MspR9I	CCNGG	2 cut(s)	886, 1222
Mva1269I	GAATGC	1 cut(s)	1365
MvaI	CCWGG	2 cut(s)	886, 1222
MwoI	GCNNNNNNNGC	7 cut(s)	11, 564, 757, 857, 1103, 1617, 1763
NdeII	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
NlaIII	CATG	4 cut(s)	315, 942, 1390, 1792
NlaIV	GGNNCC	4 cut(s)	276, 358, 936, 1656
NmuCI	GTSAC	6 cut(s)	184, 650, 1162, 1328, 1402, 1646
NspI	RCATGY	2 cut(s)	1390, 1792
PaeI	GCATGC	1 cut(s)	1390
PceI	AGGCCT	2 cut(s)	495, 567
PciI	ACATGT	1 cut(s)	1788
PciSI	GCTCTTC	1 cut(s)	1622
PctI	GAATGC	1 cut(s)	1365
PfeI	GAWTC	3 cut(s)	308, 510, 1685
PflFI	GACNNNGTC	1 cut(s)	886
PkrI	GCNGC	9 cut(s)	13, 214, 333, 532, 535, 778, 1108, 1270, 1756
PleI	GAGTC	2 cut(s)	1257, 1837
PpsI	GAGTC	2 cut(s)	1257, 1837
PpuMI	RGGWCCY	1 cut(s)	991
PscI	ACATGT	1 cut(s)	1788
Psp124BI	GAGCTC	1 cut(s)	1359
Psp5II	RGGWCCY	1 cut(s)	991
Psp6I	CCWGG	2 cut(s)	884, 1220
PspEI	GGTNACC	1 cut(s)	184
PspGI	CCWGG	2 cut(s)	884, 1220
PspN4I	GGNNCC	4 cut(s)	276, 358, 936, 1656
PspPI	GGNCC	2 cut(s)	111, 991
PspPPI	RGGWCCY	1 cut(s)	991
PstNI	CAGNNNCTG	1 cut(s)	1271
PsyI	GACNNNGTC	1 cut(s)	886
PvuII	CAGCTG	3 cut(s)	779, 845, 1271
RsaI	GTAC	4 cut(s)	163, 338, 504, 1643
RsaNI	GTAC	4 cut(s)	162, 337, 503, 1642
SacI	GAGCTC	1 cut(s)	1359
SapI	GCTCTTC	1 cut(s)	1622
SaqAI	TTAA	3 cut(s)	975, 1140, 1737
SatI	GCNGC	9 cut(s)	12, 213, 332, 531, 534, 777, 1107, 1269, 1755
Sau3AI	GATC	6 cut(s)	244, 583, 729, 733, 1561, 1585
Sau96I	GGNCC	2 cut(s)	111, 991
ScaI	AGTACT	2 cut(s)	338, 504
SchI	GAGTC	2 cut(s)	1258, 1837
ScrFI	CCNGG	2 cut(s)	886, 1222
SduI	GDGCHC	3 cut(s)	109, 1359, 1788
SexAI	ACCWGGT	1 cut(s)	884
SfaNI	GCATC	8 cut(s)	442, 466, 838, 949, 1408, 1536, 1620, 1741
SfcI	CTRYAG	1 cut(s)	1615
SinI	GGWCC	2 cut(s)	111, 991
SmlI	CTYRAG	1 cut(s)	1736
SmoI	CTYRAG	1 cut(s)	1736
SpeI	ACTAGT	1 cut(s)	154
SphI	GCATGC	1 cut(s)	1390
Sse9I	AATT	8 cut(s)	94, 351, 710, 893, 946, 972, 1233, 1518
SseBI	AGGCCT	2 cut(s)	495, 567
SsiI	CCGC	5 cut(s)	9, 51, 70, 182, 1668
SspMI	CTAG	4 cut(s)	155, 231, 401, 1178
SstI	GAGCTC	1 cut(s)	1359
StuI	AGGCCT	2 cut(s)	495, 567
StyD4I	CCNGG	2 cut(s)	884, 1220
StyI	CCWWGG	5 cut(s)	400, 1098, 1114, 1341, 1446
TaaI	ACNGT	6 cut(s)	205, 433, 548, 1438, 1598, 1646
TaiI	ACGT	1 cut(s)	178
TaqI	TCGA	5 cut(s)	197, 498, 732, 1262, 1680
TasI	AATT	8 cut(s)	94, 351, 710, 893, 946, 972, 1233, 1518
TatI	WGTACW	2 cut(s)	336, 502
TfiI	GAWTC	3 cut(s)	308, 510, 1685
Tru1I	TTAA	3 cut(s)	975, 1140, 1737
Tru9I	TTAA	3 cut(s)	975, 1140, 1737
TscAI	CASTG	3 cut(s)	1465, 1513, 1603
TseFI	GTSAC	6 cut(s)	184, 650, 1162, 1328, 1402, 1646
TseI	GCWGC	9 cut(s)	11, 212, 331, 530, 533, 776, 1106, 1268, 1754
Tsp45I	GTSAC	6 cut(s)	184, 650, 1162, 1328, 1402, 1646
TspDTI	ATGAA	7 cut(s)	281, 300, 392, 566, 792, 826, 1746
TspGWI	ACGGA	3 cut(s)	11, 318, 1118
TspRI	CASTG	3 cut(s)	1465, 1513, 1603
Tth111I	GACNNNGTC	1 cut(s)	886
Vha464I	CTTAAG	1 cut(s)	1736
VpaK11BI	GGWCC	2 cut(s)	111, 991
XagI	CCTNNNNNAGG	1 cut(s)	760
XapI	RAATTY	3 cut(s)	94, 710, 893
XceI	RCATGY	2 cut(s)	1390, 1792
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1286
XmaJI	CCTAGG	1 cut(s)	400
XmiI	GTMKAC	1 cut(s)	1123
XspI	CTAG	4 cut(s)	155, 231, 401, 1178
ZrmI	AGTACT	2 cut(s)	338, 504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.