Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002754

Physical Location & Seq

Genomic Coordinates Reverse (-)

24304090 .. 24307569

Gene Structure 3480 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1201200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCAGCCATTGCTTTGCTCCGTAACTTCCGGCGCAGCGACGTCGCGTCGGCCTCCCTCTCCGCCTACCGTTCCTTTACTAGCATTGCCAAGCCATCACCTTTGAGCCAGAAGTGGGCAAGCTTAGCAAGACCCTTCAGTTCTAGACCTGCTGGGAACGATATAATTGGGATCGATTTGGGCACAACCAACTCATGTGTTGCGGTGATGGAGGGCAAGAATCCCAAAGTTATCGAGAACTCTGAAGGAGCTCGGACCACACCATCAGTAGTTGCCATGAACCAGAAAGGAGAACTTCTTGTTGGTACTCCAGCAAAACGTCAGGCTGTGACAAACCCAACGAACACAGTTTCTGGAACCAAGCGTCTGATTGGGAGACACTTTAACGATGCCCAAACACAAAAGGAAATGAAGATGGTTCCATACAAGATTGTCAAAGCTCCAAATGGAGAAGCATGGGTTGAAGTCAATGGACAGCAGTACGCTCCAGGCCAGATTGGAGCTTTTGTTCTGACAAAGATGAAAGAAACTGCTGAGGCTTACCTTGGAAAGTCTGTTTCTAGAGCTGTGATCACGGTTCCAGCTTATTTCAATGATGCTCAGAGACAGGCCACAAAGGATGCTGGAAGAATTGCAGGGCTAACTGTAGAGAGAATCATAAACGAGCCAACTGCTGCTGCTCTTTCCTATGGTATGACCAGCAAGGAAGGCCTAATTGCTGTATTTGATCTTGGTGGTGGGACATTTGACGTATCCATTCTGGAGATATCCAATGGTGTTTTTGAGGTGAAAGCAACAAATGGTGATACATTCTTGGGAGGAGAGGATTTCGACAATGCTTTGCTGGACTTCTTAGTGAGTGAATTCAAGAGAACTGAGGGGATTGAACTTACAAAGGATAGGCTTGCCTTGCAGAGGCTTCGGGAGGCAGCTGAGAAAGCTAAGATTGAACTTTCATCTTCATCTCAAACTGAGATAAACCTTCCGTTCATCACAGCTGATGCTTCGGGCGCTAAGCATCTGAACATCACGCTGACCCGATCAAAGTTTGAAAGCCTGGTTAATCACCTGATTGAGAGGACAAAGGTACCGTGCAAGAATTGTTTGAGGGATGCCAACACGTCTATTAAGGATGTGGATGAGGTCCTTCTTGTTGGAGGAATGACTCGTGTTCCTAAAGTGCAAGAGGTAGTTGCAGAAATATTTGGAAAGAGCCCAAGCAAAGGAGTCAATCCAGATGAGGCAGTTGCTTTGGGAGCTGCCATCCAAGGTGGTATTCTTCGCGGTGATGTTAAGGAGTTGCTTCTTCTTGATGTTACTCCTCTATCACTTGGTATTGAAACACTGGGTGGTATATTTGCCAAGCTGATTGCTAGAAACACTACAATTCCAACAAAGAAGAGTCAGGTATTTTCGACAGCTGCTGACAACCAAACTCAGGTCGGTATCAAGGTGCTACAAGGGGAGAGAGAAATGGCTGCTGACAACAAGCTTCTCGGAGAGTTTGACCTTCAAGGCATCCCTCCAGCACCGAGGGGACTGCCTCAGATTGAAGTTACATTTGACATTGATGCCAACGGTATAGTAACTGTTTCTGCAAGGGACAAGGCCACTGGTAAGGAGCAACAGGTTACCATCCGCTCAAGCGCAGGGCCTACGGAGGAGGAAATTCAGAGAATGATCAAGGACGCCGAGCTTCATGCTCAAAAGGATCAGGAGAGGAAAGCTCTCGTTGATATAAAAAACAGCGCGGACACCAGCATCTACAGCATTGAGAAGAGTTTGAACGAGTACCGTGACAAAGTTCCGAGCGAAATCGCTAAAGAAATTGAGGATGCAGTTGCTGACTTGAGGACGGCAATCGGAGGGGACAGTGCTGATGAGATTAAGTCGAAGCTTGACATTGCAAACAAAGCCGTGTCCAAGATCGGTGAGCATATGTCGAAGGGTTCCGGAGGCAGTGGTGACTCTTCGTCTGGAGGCTCACAGGACGGAGGAGACGGAGTTCCTGAGGCAGAGTACCAAGAGATGAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03018 ko04212 ko05152 map03018 map04212 map05152

Pfam Domains

Protein Families

HSP70

Protein Analysis

680

Amino Acids

72.66

Weight (kDa)

5.78

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	55 - 648	1.6e-269	Hsp70 protein
MreB_Mbl	PF06723	184 - 421	1.5e-10	MreB/Mbl protein
FGGY_C	PF02782	302 - 425	8.2e-06	FGGY family of carbohydrate kinases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002754)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37910 AT4G37910 AT5G09590
fragaria_vesca	FvH4_2g11170
malus_domestica	MD05G1049700.v1.1 MD10G1056800.v1.1 MD17G1201200.v1.1
prunus_persica	Prupe.3G077000_v2.0.a1 Prupe.8G075000_v2.0.a1 Prupe.8G075000_v2.0.a1
pyrus_communis	pycom05g04150 pycom10g04370 pycom17g20500 pycom17g20510
rosa_chinensis	RchiOBHm_Chr2g0137091 RchiOBHm_Chr5g0034081 RchiOBHm_Chr5g0057671 RchiOBHm_Chr6g0269491
rosa_laevigata	RLG00000019607
rosa_multiflora	Rmu_sc0001374.1_g000006 Rmu_sc0008218.1_g000001 Rmu_ssc0000291.1_g000035
rosa_roxburghii	Rroxscaffold_1G00027300 Rroxscaffold_2G00107660 Rroxscaffold_7G00198680
rosa_rugosa	Rorug02G0174600 Rorug02G0338100 Rorug02G0338200 Rorug06G0048600
rosa_samantha	Rh1CG066900 Rh1DG071800 Rh2BG227900 Rh2CG280100 Rh6AG168500 Rh6BG173900 Rh6CG168200 Rh6DG160200
rosa_wichuraiana	Rw2G031680 Rw6G014510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	45
Acc36I	ACCTGC	1 cut(s)	157
Acc65I	GGTACC	1 cut(s)	1087
AccB1I	GGYRCC	1 cut(s)	1087
AccBSI	CCGCTC	1 cut(s)	1641
AccII	CGCG	3 cut(s)	47, 1284, 1751
AccIII	TCCGGA	1 cut(s)	1952
AciI	CCGC	5 cut(s)	63, 203, 1284, 1639, 1751
AclWI	GGATC	2 cut(s)	179, 1719
AcsI	RAATTY	2 cut(s)	863, 1668
AcuI	CTGAAG	2 cut(s)	121, 264
AcyI	GRCGYC	2 cut(s)	42, 1689
AdeI	CACNNNGTG	1 cut(s)	1349
AfaI	GTAC	5 cut(s)	307, 482, 1089, 1793, 2021
AfiI	CCNNNNNNNGG	4 cut(s)	115, 915, 1223, 1438
AflIII	ACRYGT	1 cut(s)	1119
AhdI	GACNNNNNGTC	2 cut(s)	46, 1972
AjiI	CACGTC	1 cut(s)	1122
AjnI	CCWGG	2 cut(s)	487, 1056
AjuI	GAANNNNNNNTTGG	4 cut(s)	285, 317, 1260, 1292
AloI	GAACNNNNNNTCC	4 cut(s)	492, 524, 1989, 2021
Alw21I	GWGCWC	1 cut(s)	253
Alw26I	GTCTC	3 cut(s)	370, 598, 1992
AlwI	GGATC	2 cut(s)	179, 1719
AlwNI	CAGNNNCTG	3 cut(s)	353, 1424, 1844
Aor13HI	TCCGGA	1 cut(s)	1952
AoxI	GGCC	6 cut(s)	51, 490, 609, 709, 1608, 1652
ApeKI	GCWGC	8 cut(s)	5, 36, 674, 677, 929, 1259, 1421, 1478
ApoI	RAATTY	2 cut(s)	863, 1668
Asp718I	GGTACC	1 cut(s)	1087
AspLEI	GCGC	4 cut(s)	36, 1013, 1649, 1751
AspS9I	GGNCC	3 cut(s)	255, 1144, 1652
AsuHPI	GGTGA	8 cut(s)	90, 217, 799, 815, 1058, 1298, 1943, 1976
AvaII	GGWCC	2 cut(s)	255, 1144
AxyI	CCTNAGG	1 cut(s)	2010
BaeGI	GKGCMC	1 cut(s)	185
BaeI	ACNNNNGTAYC	2 cut(s)	1071, 1104
BanI	GGYRCC	1 cut(s)	1087
BanII	GRGCYC	2 cut(s)	253, 1217
BauI	CACGAG	1 cut(s)	1167
Bbv12I	GWGCWC	1 cut(s)	253
BbvCI	CCTCAGC	1 cut(s)	534
BbvI	GCAGC	8 cut(s)	17, 48, 661, 664, 941, 1246, 1408, 1465
BccI	CCATC	6 cut(s)	103, 202, 271, 409, 1271, 1643
BceAI	ACGGC	2 cut(s)	1872, 1901
BciT130I	CCWGG	2 cut(s)	489, 1058
BciVI	GTATCC	1 cut(s)	763
BclI	TGATCA	2 cut(s)	570, 1680
BcoDI	GTCTC	3 cut(s)	370, 598, 1992
BfaI	CTAG	4 cut(s)	81, 144, 561, 1374
BfmI	CTRYAG	2 cut(s)	645, 1765
BfoI	RGCGCY	1 cut(s)	1014
BfuAI	ACCTGC	1 cut(s)	157
BfuI	GTATCC	1 cut(s)	763
BisI	GCNGC	8 cut(s)	6, 37, 675, 678, 930, 1260, 1422, 1479
BlpI	GCTNAGC	2 cut(s)	124, 1014
BlsI	GCNGC	8 cut(s)	7, 38, 676, 679, 931, 1261, 1423, 1480
Bme1390I	CCNGG	2 cut(s)	489, 1058
Bme18I	GGWCC	2 cut(s)	255, 1144
BmeRI	GACNNNNNGTC	2 cut(s)	46, 1972
BmgBI	CACGTC	1 cut(s)	1122
BmgT120I	GGNCC	3 cut(s)	255, 1144, 1652
BmiI	GGNNCC	5 cut(s)	358, 420, 579, 1089, 1951
BmrFI	CCNGG	2 cut(s)	489, 1058
BmrI	ACTGGG	1 cut(s)	1355
BmuI	ACTGGG	1 cut(s)	1355
BplI	GAGNNNNNCTC	2 cut(s)	1711, 1743
BpmI	CTGGAG	5 cut(s)	294, 471, 782, 1509, 1998
Bpu10I	CCTNAGC	1 cut(s)	534
Bpu1102I	GCTNAGC	2 cut(s)	124, 1014
BpuEI	CTTGAG	2 cut(s)	1627, 1870
Bsa29I	ATCGAT	1 cut(s)	174
BsaHI	GRCGYC	2 cut(s)	42, 1689
BsaJI	CCNNGG	3 cut(s)	544, 1267, 1532
BsaWI	WCCGGW	1 cut(s)	1952
BsaXI	ACNNNNNCTCC	4 cut(s)	492, 522, 1989, 2019
Bsc4I	CCNNNNNNNGG	4 cut(s)	115, 915, 1223, 1438
Bse1I	ACTGG	2 cut(s)	1350, 1618
Bse21I	CCTNAGG	1 cut(s)	2010
Bse3DI	GCAATG	3 cut(s)	9, 84, 1902
BseAI	TCCGGA	1 cut(s)	1952
BseBI	CCWGG	2 cut(s)	489, 1058
BseCI	ATCGAT	1 cut(s)	174
BseDI	CCNNGG	3 cut(s)	544, 1267, 1532
BseGI	GGATG	8 cut(s)	625, 1117, 1138, 1144, 1263, 1518, 1635, 1840
BseLI	CCNNNNNNNGG	4 cut(s)	115, 915, 1223, 1438
BseMI	GCAATG	3 cut(s)	9, 84, 1902
BseMII	CTCAG	8 cut(s)	525, 614, 867, 924, 963, 1451, 1559, 2001
BseNI	ACTGG	2 cut(s)	1350, 1618
BseRI	GAGGAG	4 cut(s)	834, 1311, 1676, 2010
BseSI	GKGCMC	1 cut(s)	185
BseXI	GCAGC	8 cut(s)	17, 48, 661, 664, 941, 1246, 1408, 1465
BseYI	CCCAGC	1 cut(s)	152
Bsh1236I	CGCG	3 cut(s)	47, 1284, 1751
BshFI	GGCC	6 cut(s)	53, 492, 611, 711, 1610, 1654
BshNI	GGYRCC	1 cut(s)	1087
BshVI	ATCGAT	1 cut(s)	174
BsiHKAI	GWGCWC	1 cut(s)	253
BsiSI	CCGG	2 cut(s)	31, 1953
BslFI	GGGAC	4 cut(s)	754, 1551, 1616, 1883
BslI	CCNNNNNNNGG	4 cut(s)	115, 915, 1223, 1438
BsmAI	GTCTC	3 cut(s)	370, 598, 1992
BsmBI	CGTCTC	1 cut(s)	1992
BsmFI	GGGAC	4 cut(s)	754, 1551, 1616, 1883
BsnI	GGCC	6 cut(s)	53, 492, 611, 711, 1610, 1654
Bsp1286I	GDGCHC	3 cut(s)	185, 253, 1217
Bsp13I	TCCGGA	1 cut(s)	1952
Bsp143I	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
Bsp1720I	GCTNAGC	2 cut(s)	124, 1014
BspACI	CCGC	5 cut(s)	63, 203, 1284, 1639, 1751
BspANI	GGCC	6 cut(s)	53, 492, 611, 711, 1610, 1654
BspCNI	CTCAG	8 cut(s)	526, 613, 868, 925, 964, 1450, 1558, 2002
BspDI	ATCGAT	1 cut(s)	174
BspEI	TCCGGA	1 cut(s)	1952
BspFNI	CGCG	3 cut(s)	47, 1284, 1751
BspLI	GGNNCC	5 cut(s)	358, 420, 579, 1089, 1951
BspMI	ACCTGC	1 cut(s)	157
BspPI	GGATC	2 cut(s)	179, 1719
BspT107I	GGYRCC	1 cut(s)	1087
BsrBI	CCGCTC	1 cut(s)	1641
BsrDI	GCAATG	3 cut(s)	9, 84, 1902
BsrI	ACTGG	2 cut(s)	1350, 1618
BssECI	CCNNGG	3 cut(s)	544, 1267, 1532
BssMI	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
BssNI	GRCGYC	2 cut(s)	42, 1689
BssSI	CACGAG	1 cut(s)	1167
BssT1I	CCWWGG	2 cut(s)	544, 1267
Bst2BI	CACGAG	1 cut(s)	1167
Bst2UI	CCWGG	2 cut(s)	489, 1058
Bst4CI	ACNGT	9 cut(s)	71, 349, 577, 646, 1092, 1580, 1591, 1796, 1874
Bst6I	CTCTTC	3 cut(s)	1394, 1772, 1975
BstACI	GRCGYC	2 cut(s)	42, 1689
BstAPI	GCANNNNNTGC	1 cut(s)	11
BstC8I	GCNNGC	2 cut(s)	121, 906
BstEII	GGTNACC	1 cut(s)	1630
BstENI	CCTNNNNNAGG	1 cut(s)	913
BstF5I	GGATG	8 cut(s)	625, 1117, 1138, 1144, 1263, 1518, 1635, 1840
BstFNI	CGCG	3 cut(s)	47, 1284, 1751
BstH2I	RGCGCY	1 cut(s)	1014
BstHHI	GCGC	4 cut(s)	36, 1013, 1649, 1751
BstKTI	GATC	7 cut(s)	174, 573, 730, 1043, 1683, 1714, 1929
BstMAI	GTCTC	3 cut(s)	370, 598, 1992
BstMBI	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 125, 708, 910, 938, 1010, 1256, 1767, 1913
BstNI	CCWGG	2 cut(s)	489, 1058
BstPI	GGTNACC	1 cut(s)	1630
BstSCI	CCNGG	2 cut(s)	487, 1056
BstSFI	CTRYAG	2 cut(s)	645, 1765
BstSLI	GKGCMC	1 cut(s)	185
BstUI	CGCG	3 cut(s)	47, 1284, 1751
BstV1I	GCAGC	8 cut(s)	17, 48, 661, 664, 941, 1246, 1408, 1465
Bsu15I	ATCGAT	1 cut(s)	174
Bsu36I	CCTNAGG	1 cut(s)	2010
BsuI	GTATCC	1 cut(s)	763
BsuRI	GGCC	6 cut(s)	53, 492, 611, 711, 1610, 1654
BsuTUI	ATCGAT	1 cut(s)	174
BtrI	CACGTC	1 cut(s)	1122
BtsCI	GGATG	8 cut(s)	625, 1117, 1138, 1144, 1263, 1518, 1635, 1840
BtsI	GCAGTG	1 cut(s)	1966
BtsIMutI	CAGTG	4 cut(s)	1343, 1611, 1879, 1966
BveI	ACCTGC	1 cut(s)	157
Cac8I	GCNNGC	2 cut(s)	121, 906
CaiI	CAGNNNCTG	3 cut(s)	353, 1424, 1844
CfoI	GCGC	4 cut(s)	36, 1013, 1649, 1751
Cfr13I	GGNCC	3 cut(s)	255, 1144, 1652
ClaI	ATCGAT	1 cut(s)	174
CseI	GACGC	3 cut(s)	36, 353, 1697
Csp6I	GTAC	5 cut(s)	306, 481, 1088, 1792, 2020
CviAII	CATG	4 cut(s)	195, 277, 456, 1700
CviQI	GTAC	5 cut(s)	306, 481, 1088, 1792, 2020
DpnI	GATC	7 cut(s)	173, 572, 729, 1042, 1682, 1713, 1928
DpnII	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
DraIII	CACNNNGTG	1 cut(s)	1349
DriI	GACNNNNNGTC	2 cut(s)	46, 1972
Eam1104I	CTCTTC	3 cut(s)	1394, 1772, 1975
Eam1105I	GACNNNNNGTC	2 cut(s)	46, 1972
EarI	CTCTTC	3 cut(s)	1394, 1772, 1975
EciI	GGCGGA	1 cut(s)	52
Ecl136II	GAGCTC	1 cut(s)	251
Eco130I	CCWWGG	2 cut(s)	544, 1267
Eco147I	AGGCCT	1 cut(s)	711
Eco24I	GRGCYC	2 cut(s)	253, 1217
Eco32I	GATATC	1 cut(s)	768
Eco47I	GGWCC	2 cut(s)	255, 1144
Eco53kI	GAGCTC	1 cut(s)	251
Eco57I	CTGAAG	2 cut(s)	121, 264
Eco81I	CCTNAGG	1 cut(s)	2010
Eco91I	GGTNACC	1 cut(s)	1630
EcoICRI	GAGCTC	1 cut(s)	251
EcoNI	CCTNNNNNAGG	1 cut(s)	913
EcoO109I	RGGNCCY	2 cut(s)	1144, 1652
EcoO65I	GGTNACC	1 cut(s)	1630
EcoRI	GAATTC	1 cut(s)	863
EcoRII	CCWGG	2 cut(s)	487, 1056
EcoRV	GATATC	1 cut(s)	768
EcoT14I	CCWWGG	2 cut(s)	544, 1267
EcoT38I	GRGCYC	2 cut(s)	253, 1217
ErhI	CCWWGG	2 cut(s)	544, 1267
Esp3I	CGTCTC	1 cut(s)	1992
FaeI	CATG	4 cut(s)	198, 280, 459, 1703
FalI	AAGNNNNNCTT	2 cut(s)	279, 311
FaqI	GGGAC	4 cut(s)	754, 1551, 1616, 1883
FatI	CATG	4 cut(s)	194, 276, 455, 1699
FauNDI	CATATG	1 cut(s)	1938
FbaI	TGATCA	2 cut(s)	570, 1680
Fnu4HI	GCNGC	8 cut(s)	6, 37, 675, 678, 930, 1260, 1422, 1479
FokI	GGATG	8 cut(s)	632, 1124, 1145, 1151, 1250, 1505, 1622, 1847
FriOI	GRGCYC	2 cut(s)	253, 1217
Fsp4HI	GCNGC	8 cut(s)	6, 37, 675, 678, 930, 1260, 1422, 1479
FspBI	CTAG	4 cut(s)	81, 144, 561, 1374
GlaI	GCGC	4 cut(s)	35, 1012, 1648, 1750
GluI	GCNGC	8 cut(s)	6, 37, 675, 678, 930, 1260, 1422, 1479
GsaI	CCCAGC	1 cut(s)	156
GsuI	CTGGAG	5 cut(s)	294, 471, 782, 1509, 1998
HaeII	RGCGCY	1 cut(s)	1014
HaeIII	GGCC	6 cut(s)	53, 492, 611, 711, 1610, 1654
HapII	CCGG	2 cut(s)	31, 1953
HgaI	GACGC	3 cut(s)	36, 353, 1697
HhaI	GCGC	4 cut(s)	36, 1013, 1649, 1751
Hin1I	GRCGYC	2 cut(s)	42, 1689
Hin1II	CATG	4 cut(s)	198, 280, 459, 1703
Hin6I	GCGC	4 cut(s)	34, 1011, 1647, 1749
HinP1I	GCGC	4 cut(s)	34, 1011, 1647, 1749
HindIII	AAGCTT	3 cut(s)	121, 1490, 1895
HinfI	GANTC	6 cut(s)	220, 654, 1165, 1227, 1402, 1967
HpaII	CCGG	2 cut(s)	31, 1953
HphI	GGTGA	8 cut(s)	90, 217, 799, 815, 1058, 1298, 1943, 1976
Hpy99I	CGWCG	3 cut(s)	44, 47, 52
HpyAV	CCTTC	7 cut(s)	145, 239, 701, 992, 1157, 1520, 1939
HpyCH4III	ACNGT	9 cut(s)	71, 349, 577, 646, 1092, 1580, 1591, 1796, 1874
HpyCH4IV	ACGT	4 cut(s)	42, 319, 750, 1121
HpyCH4V	TGCA	8 cut(s)	635, 913, 1095, 1183, 1196, 1598, 1838, 1907
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 125, 708, 910, 938, 1010, 1256, 1767, 1913
HpySE526I	ACGT	4 cut(s)	42, 319, 750, 1121
Hsp92I	GRCGYC	2 cut(s)	42, 1689
Hsp92II	CATG	4 cut(s)	198, 280, 459, 1703
HspAI	GCGC	4 cut(s)	34, 1011, 1647, 1749
Kpn2I	TCCGGA	1 cut(s)	1952
KpnI	GGTACC	1 cut(s)	1091
Ksp22I	TGATCA	2 cut(s)	570, 1680
Kzo9I	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
LmnI	GCTCC	7 cut(s)	24, 248, 445, 490, 500, 1256, 1621
Lsp1109I	GCAGC	8 cut(s)	17, 48, 661, 664, 941, 1246, 1408, 1465
MaeI	CTAG	4 cut(s)	81, 144, 561, 1374
MaeII	ACGT	4 cut(s)	42, 319, 750, 1121
MaeIII	GTNAC	8 cut(s)	23, 328, 1315, 1555, 1585, 1630, 1796, 1964
MalI	GATC	7 cut(s)	173, 572, 729, 1042, 1682, 1713, 1928
MbiI	CCGCTC	1 cut(s)	1641
MboI	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
MboII	GAAGA	8 cut(s)	424, 639, 951, 1271, 1298, 1411, 1789, 1962
MhlI	GDGCHC	3 cut(s)	185, 253, 1217
MluCI	AATT	8 cut(s)	165, 630, 714, 863, 1099, 1386, 1668, 1827
MlyI	GAGTC	4 cut(s)	1159, 1236, 1411, 1961
MmeI	TCCRAC	2 cut(s)	1135, 1415
MroI	TCCGGA	1 cut(s)	1952
MseI	TTAA	5 cut(s)	384, 1062, 1128, 1293, 1887
MspA1I	CMGCKG	3 cut(s)	932, 998, 1421
MspI	CCGG	2 cut(s)	31, 1953
MspR9I	CCNGG	2 cut(s)	489, 1058
MvaI	CCWGG	2 cut(s)	489, 1058
MvnI	CGCG	3 cut(s)	47, 1284, 1751
MwoI	GCNNNNNNNGC	9 cut(s)	11, 125, 708, 910, 938, 1010, 1256, 1767, 1913
NdeI	CATATG	1 cut(s)	1938
NdeII	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
NlaIII	CATG	4 cut(s)	198, 280, 459, 1703
NlaIV	GGNNCC	5 cut(s)	358, 420, 579, 1089, 1951
NmeAIII	GCCGAG	1 cut(s)	1717
NmuCI	GTSAC	3 cut(s)	328, 1796, 1964
PceI	AGGCCT	1 cut(s)	711
PfeI	GAWTC	2 cut(s)	220, 654
PkrI	GCNGC	8 cut(s)	7, 38, 676, 679, 931, 1261, 1423, 1480
PleI	GAGTC	4 cut(s)	1159, 1235, 1410, 1961
PpsI	GAGTC	4 cut(s)	1159, 1235, 1410, 1961
PpuMI	RGGWCCY	1 cut(s)	1144
Psp124BI	GAGCTC	1 cut(s)	253
Psp5II	RGGWCCY	1 cut(s)	1144
Psp6I	CCWGG	2 cut(s)	487, 1056
PspEI	GGTNACC	1 cut(s)	1630
PspFI	CCCAGC	1 cut(s)	152
PspGI	CCWGG	2 cut(s)	487, 1056
PspN4I	GGNNCC	5 cut(s)	358, 420, 579, 1089, 1951
PspPI	GGNCC	3 cut(s)	255, 1144, 1652
PspPPI	RGGWCCY	1 cut(s)	1144
PstNI	CAGNNNCTG	3 cut(s)	353, 1424, 1844
PvuII	CAGCTG	3 cut(s)	932, 998, 1421
RsaI	GTAC	5 cut(s)	307, 482, 1089, 1793, 2021
RsaNI	GTAC	5 cut(s)	306, 481, 1088, 1792, 2020
SacI	GAGCTC	1 cut(s)	253
SaqAI	TTAA	5 cut(s)	384, 1062, 1128, 1293, 1887
SatI	GCNGC	8 cut(s)	6, 37, 675, 678, 930, 1260, 1422, 1479
Sau3AI	GATC	7 cut(s)	171, 570, 727, 1040, 1680, 1711, 1926
Sau96I	GGNCC	3 cut(s)	255, 1144, 1652
SchI	GAGTC	4 cut(s)	1159, 1236, 1411, 1961
ScrFI	CCNGG	2 cut(s)	489, 1058
SduI	GDGCHC	3 cut(s)	185, 253, 1217
SfcI	CTRYAG	2 cut(s)	645, 1765
SinI	GGWCC	2 cut(s)	255, 1144
SmlI	CTYRAG	2 cut(s)	1642, 1849
SmoI	CTYRAG	2 cut(s)	1642, 1849
Sse9I	AATT	8 cut(s)	165, 630, 714, 863, 1099, 1386, 1668, 1827
SseBI	AGGCCT	1 cut(s)	711
SsiI	CCGC	5 cut(s)	63, 203, 1284, 1639, 1751
SspI	AATATT	1 cut(s)	1203
SspMI	CTAG	4 cut(s)	81, 144, 561, 1374
SstI	GAGCTC	1 cut(s)	253
StuI	AGGCCT	1 cut(s)	711
StyD4I	CCNGG	2 cut(s)	487, 1056
StyI	CCWWGG	2 cut(s)	544, 1267
TaaI	ACNGT	9 cut(s)	71, 349, 577, 646, 1092, 1580, 1591, 1796, 1874
TaiI	ACGT	4 cut(s)	45, 322, 753, 1124
TaqI	TCGA	6 cut(s)	174, 234, 831, 1415, 1892, 1943
TasI	AATT	8 cut(s)	165, 630, 714, 863, 1099, 1386, 1668, 1827
TfiI	GAWTC	2 cut(s)	220, 654
Tru1I	TTAA	5 cut(s)	384, 1062, 1128, 1293, 1887
Tru9I	TTAA	5 cut(s)	384, 1062, 1128, 1293, 1887
TscAI	CASTG	4 cut(s)	1350, 1618, 1879, 1966
TseFI	GTSAC	3 cut(s)	328, 1796, 1964
TseI	GCWGC	8 cut(s)	5, 36, 674, 677, 929, 1259, 1421, 1478
Tsp45I	GTSAC	3 cut(s)	328, 1796, 1964
TspDTI	ATGAA	7 cut(s)	293, 425, 536, 945, 951, 979, 1688
TspGWI	ACGGA	5 cut(s)	11, 975, 1673, 2007, 2016
TspRI	CASTG	4 cut(s)	1350, 1618, 1879, 1966
VpaK11BI	GGWCC	2 cut(s)	255, 1144
XagI	CCTNNNNNAGG	1 cut(s)	913
XapI	RAATTY	2 cut(s)	863, 1668
XbaI	TCTAGA	2 cut(s)	143, 560
XspI	CTAG	4 cut(s)	81, 144, 561, 1374
ZraI	GACGTC	1 cut(s)	43

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.