Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001904

Physical Location & Seq

Genomic Coordinates Reverse (-)

19538400 .. 19546536

Gene Structure 8137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1092200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGGCCGATACTGCACAACTGGATATTACTTCAGAATGTGATAGCAGTTATGCAACAAAATGCCAAGAAGAAGGTACGTCAGGACAGATGCATGAAATTGGTTCTGAAAGTCAATGTAGTGAACCAACCAAACAAAACATTGGGTGCGCCATTGTCCAGAATGAGCTACGGGAAATCTGCAATGCTGGTGAACAATCACAGTCATTATCTGAAAATGTGACTGAGAATTCTCATCTTGAACAATTGGGATTGCCTTCTGAAAACGTGAGCAAGAGTAGTCAAAGTGGAGCTGAAAATGTTTTTGCAAAACAGGGATATCAATCCATTTCTGGATATAAACAAGATGAATCGTTACAAACCACAGCTGCAGTGTTTCCCTGTACCATGAAGGACCAACTGCAATCATTTTCTGGAAACATGAGCAAGAATTCTCTTATTGATCAAATGGAAATGCCTTGTGAAGATCTGGGTGTGAATGGTCCAACCCATAAAACTTCATTCTCTGGACAAATGCCATTGGAACAGAAAAATGATTGTGGTTTTGGAACTTCATCTAGTGAACTAGCTGAAGAAAAGCATCCTTCTGCCTCAGATCATGTACAAAATGAACAGTTACAAGCAATTATTCAAGTACCCATCTGTGGTAGCAATCAACACTTGCAGTCATCTAGTGAAAATGTGAACAGGACTTCTCTTAACGAACAAGCAGGACTGCCATCTGAAGATCTGTCAAAGACTTGTCAGACTGACAAAGTTTCATGCTCTAACCAGACTACATTACAGGAGATCAATGAATTTGGGTGTGGAAGTGTACACGGTGAACCAGAAACACAAAAAGATCAGCTTGACTCTGTACCTGCTCATAATAATGAAGTTACAACTACTGAAGCAGCACCCAGCTCTATTGTTTTTGAACAATCAAGGCCTTGTATTGAAGCTATGACCCAGGATTCTCCTACTGGACATTTGGAACTACCCCTTAAAGATGCAAGCAAGAGTCCTCCTATTGATAAAGAAATGGAACAACTTCCAGCAGATGTGACCCAAAATTCTAGTCTTGAAAAGACGGAAAAGCCATCTAAGAATGCACCGAAGGATAAGCAGAATCCAAAATCAAGAAAGAAGAAATATGTGTCAAAATCTTCCGTTGGCAGTGACAGAGTTCTGCGCTCAAAGACAGGGGAGAAGACTAAAAATCCTAAATTAAGTAATGATGTTTCAACTCTTGAATCAAGTAATAGTGTTGCAAATCCAAGTAATGTTGAAGGCAAAAGAAGGAAGAAGAGAAAGAAGAGACAACTGAATAAAGTCATAGATGATGAATTTTCAAGAGTCAGGAAACATCTGAGATATTTGCTGAACCGGATAAGCTATGAGAAATCTCTGATTGATGCTTACTCCGGCGAAGGATGGAAAGGATCCAGCCTAGAAAAACTGAAGCCAGAGAAGGAGCTTCAAAGAGCGACTTCTGAAATACTTCAACGCAAGTTGAAGATAAGAGATCTATTTCAACGTCTCGATTCGCTATGTTCTGAAGGAATGTTTCCAGAATCTTTATTTGATTCCGAAGGACAGATTGACAGTGAGGATATATTCTGTGCAAAATGTGGATCCAAGGACGTTTCTCTTCAGAATGATATTATACTCTGTGATGGCGCTTGTGACCGTGGGTTCCACCAATTCTGTTTAGAACCACCACTATTAAGTGAAGACATTCCACCCGACGACGAGGGTTGGCTATGCCCTGGATGTGACTGCAAAGTTGATTGCTTTGATTTGCTTAATGACTCTCAAGGAACAGATCTTTCTGTTGCCGACAGCTGGGAGAAGGTATTTCCTGAAGCGGCTGCTGCAGCATCAGGACATAATCAGGAGCACACCCATGGACTTCCATCAGATGATTCTGATGATAATGATTATGACCCTGATGGTCCAGAAACAGATGATGAAGTTCAGGGAGAAGAATCAAGTTCTGATGATGAATCTAAATATGCATCTGCATCTGATGGACTAGAGACTCCAAAAAATAATGACGAGCAGTACTTAGGACTTCCTTCTGACGATTCAGAGGATGATGACTATAATCCTGATGCTCCAGAAGTCACTGAGGAACTTAAGAAGGAAAGTTCAAGTTCTGATTTTACATCTGACTCTGAGGATCTTGGAGCTTCTCTTGACGACAATAATATGTTTTCCGAAGATGTTGAAAGTCCCAAGTCAATGTCATTGGACGAAAGTGGGCCTCTTAGAGGCTCCGGCAAACAAAGTTCTAGACGTGGGCAAAAGAAACAACCTTTAAAAGATGAGTTATTATCTTTATTAGAGTCGGGTCCTGGCCAAGCTGGTGCAGCTCCTGTTTCTGGGAAAAGGCACATAGAAAGGTTGAACTACAAAAAGCTGCATGATGAGACATATGGAAATGTTCGTACTGATTCAAGTGATGATGAAGAGTGGAATGATACTGCTGGACCGAGGAAAAGGAAGAAAGTTACTACACAAGCTCCTACGATGTCACCAAATGGAGACTCATCAAACGTTAAGAATGGAATGATCACCAACAATATAAAACATGATCTAGATGAGAATGAGAATACTCCTAAAAGAACTCCTCGCAGAAATAAAAATACTCCTAAGAGAGCTCATCGAAAGTCAAAGGTTGAAGATACAAGTAATTTGTCGAACAAATCACAAAAAGGCTCTACTCAGTCCGCTTCTACTAGTGAGCAAGGTGGATCATCAAGATCAACATATAGAAAACTAGGGGAAGCTGCAACTCAGCGACTATCCAAATCGTTCAAGGAAAACCACTATCCAGACCGATCTATGAAGGAAAGTCTGGCCCGAGAACTAGGAATAATGGCTAAGCAGGTAAGCAAATGGTTTGAAAACGCTCGCCACTTTTGGAAAGTGAGTGTGGATAAGAGTGCTGCAGGAAATGGCACTCCCTTGCCTCAAACAAATGGAAAGCAACTTGAAAAAGGTGATACACCTATTGGAGATTCTGATCAGAGTGGAGCTCAAAACAAGGAATTACCCAGGACAAATGATCCTATGACAGGTTCCTGCAGCGGGGATGCAAAAGATGGTGAATTGGTGACTCCGAAAAGCAGTAAACGAAAGGCTATAACTCCAAACAATAGAAAGAGAAGGCGTAAATCAGATGATCCTGATCCAGAAAACAAGACACCAGAAACTAATAGAAAAGCTCCTGTTCTTACCGATCTTCCATTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010646 GO:0010647 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox PHD

Protein Analysis

1079

Amino Acids

118.52

Weight (kDa)

5.01

Isoelectric Point (pI)

56.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHD	PF00628	533 - 584	2.9e-09	PHD-finger
Homeodomain	PF00046	921 - 969	1.8e-10	Homeodomain
Homeobox_KN	PF05920	935 - 965	6e-07	Homeobox KN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001904)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19510 AT3G19510 AT3G19530
fragaria_vesca	FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760 FvH4_2g04760
malus_domestica	MD05G1092200.v1.1 MD10G1100500.v1.1
prunus_persica	Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1 Prupe.8G137800_v2.0.a1
pyrus_communis	pycom05g09180 pycom10g08610
rosa_chinensis	RchiOBHm_Chr6g0254411
rosa_laevigata	RLG00000014914
rosa_multiflora	Rmu_co8217068.1_g000001 Rmu_co8296759.1_g000001 Rmu_co8300769.1_g000001 Rmu_sc0009578.1_g000001 Rmu_ssc0000121.1_g000001 Rmu_ssc0000481.1_g000002
rosa_roxburghii	Rroxscaffold_7G00210820
rosa_rugosa	Rorug05G0552100
rosa_samantha	Rh6AG069200 Rh6BG061900 Rh6CG062000 Rh6DG059000
rosa_wichuraiana	Rw6G006080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	865, 2857
AciI	CCGC	3 cut(s)	1844, 2709, 3069
AclI	AACGTT	1 cut(s)	2535
AclWI	GGATC	9 cut(s)	1413, 1426, 1605, 1618, 2166, 2740, 3041, 3158, 3164
AcoI	YGGCCR	2 cut(s)	3, 2335
AcsI	RAATTY	5 cut(s)	226, 427, 794, 1048, 1322
AcuI	CTGAAG	8 cut(s)	15, 588, 741, 906, 1457, 1554, 1613, 1860
AfaI	GTAC	8 cut(s)	76, 382, 600, 633, 813, 855, 2042, 2428
AfiI	CCNNNNNNNGG	6 cut(s)	641, 1821, 2249, 2360, 3056, 3069
AflII	CTTAAG	1 cut(s)	2114
AhlI	ACTAGT	1 cut(s)	2717
AjiI	CACGTC	1 cut(s)	2276
AjnI	CCWGG	4 cut(s)	945, 1744, 2332, 3035
Alw21I	GWGCWC	3 cut(s)	1878, 2641, 3019
Alw26I	GTCTC	5 cut(s)	1288, 1520, 2009, 2402, 2517
AlwI	GGATC	9 cut(s)	1413, 1426, 1605, 1618, 2166, 2740, 3041, 3158, 3164
AlwNI	CAGNNNCTG	1 cut(s)	2354
Ama87I	CYCGRG	1 cut(s)	2841
AoxI	GGCC	5 cut(s)	3, 923, 2240, 2335, 2838
ApoI	RAATTY	5 cut(s)	226, 427, 794, 1048, 1322
ArsI	GACNNNNNNTTYG	2 cut(s)	1039, 1071
Asp700I	GAANNNNTTC	3 cut(s)	1026, 1476, 1713
AspLEI	GCGC	3 cut(s)	149, 1170, 1658
AspS9I	GGNCC	7 cut(s)	391, 479, 1931, 2240, 2330, 2468, 2839
AsuHPI	GGTGA	7 cut(s)	200, 830, 2505, 2545, 2993, 3098, 3106
AvaI	CYCGRG	1 cut(s)	2841
AvaII	GGWCC	5 cut(s)	391, 479, 1931, 2330, 2468
BalI	TGGCCA	1 cut(s)	2337
BamHI	GGATCC	2 cut(s)	1418, 1610
BanII	GRGCYC	2 cut(s)	2641, 3019
BbsI	GAAGAC	2 cut(s)	1193, 1716
Bbv12I	GWGCWC	3 cut(s)	1878, 2641, 3019
BccI	CCATC	9 cut(s)	644, 724, 1084, 1404, 1646, 1900, 1922, 2000, 3077
BciT130I	CCWGG	4 cut(s)	947, 1746, 2334, 3037
BclI	TGATCA	3 cut(s)	439, 2550, 3004
BcoDI	GTCTC	5 cut(s)	1288, 1520, 2009, 2402, 2517
BcuI	ACTAGT	1 cut(s)	2717
BfmI	CTRYAG	4 cut(s)	366, 1851, 2928, 3064
BfoI	RGCGCY	1 cut(s)	1659
BfrI	CTTAAG	1 cut(s)	2114
BfuAI	ACCTGC	2 cut(s)	865, 2857
BglII	AGATCT	4 cut(s)	463, 724, 1501, 1801
BlpI	GCTNAGC	1 cut(s)	2862
BmcAI	AGTACT	1 cut(s)	2042
Bme1390I	CCNGG	4 cut(s)	947, 1746, 2334, 3037
Bme18I	GGWCC	5 cut(s)	391, 479, 1931, 2330, 2468
BmeT110I	CYCGRG	1 cut(s)	2841
BmgBI	CACGTC	1 cut(s)	2276
BmgT120I	GGNCC	7 cut(s)	391, 479, 1931, 2240, 2330, 2468, 2839
BmiI	GGNNCC	6 cut(s)	1420, 1612, 1673, 2254, 2331, 3061
BmrFI	CCNGG	4 cut(s)	947, 1746, 2334, 3037
BmsI	GCATC	9 cut(s)	78, 586, 976, 1381, 1865, 2003, 2009, 2080, 3064
BpiI	GAAGAC	2 cut(s)	1193, 1716
BplI	GAGNNNNNCTC	2 cut(s)	3001, 3033
BpmI	CTGGAG	1 cut(s)	2079
Bpu1102I	GCTNAGC	1 cut(s)	2862
BpuEI	CTTGAG	1 cut(s)	1776
BsaBI	GATNNNNATC	3 cut(s)	319, 3003, 3168
BsaJI	CCNNGG	7 cut(s)	945, 1614, 1666, 1744, 1882, 2471, 3035
BsaWI	WCCGGW	1 cut(s)	1362
BsaXI	ACNNNNNCTCC	2 cut(s)	2485, 2515
Bsc4I	CCNNNNNNNGG	6 cut(s)	641, 1821, 2249, 2360, 3056, 3069
Bse1I	ACTGG	2 cut(s)	24, 964
Bse3DI	GCAATG	1 cut(s)	187
Bse8I	GATNNNNATC	3 cut(s)	319, 3003, 3168
BseBI	CCWGG	4 cut(s)	947, 1746, 2334, 3037
BseDI	CCNNGG	7 cut(s)	945, 1614, 1666, 1744, 1882, 2471, 3035
BseGI	GGATG	5 cut(s)	577, 1415, 1754, 2077, 3079
BseJI	GATNNNNATC	3 cut(s)	319, 3003, 3168
BseLI	CCNNNNNNNGG	6 cut(s)	641, 1821, 2249, 2360, 3056, 3069
BseMI	GCAATG	1 cut(s)	187
BseMII	CTCAG	7 cut(s)	213, 603, 1337, 2097, 2145, 2717, 2789
BseNI	ACTGG	2 cut(s)	24, 964
BseRI	GAGGAG	1 cut(s)	2598
BseYI	CCCAGC	2 cut(s)	896, 1821
BsgI	GTGCAG	1 cut(s)	2367
BshFI	GGCC	5 cut(s)	5, 925, 2242, 2337, 2840
BsiHKAI	GWGCWC	3 cut(s)	1878, 2641, 3019
BsiHKCI	CYCGRG	1 cut(s)	2841
BsiSI	CCGG	3 cut(s)	1363, 1401, 2256
BslFI	GGGAC	1 cut(s)	2196
BslI	CCNNNNNNNGG	6 cut(s)	641, 1821, 2249, 2360, 3056, 3069
BsmAI	GTCTC	5 cut(s)	1288, 1520, 2009, 2402, 2517
BsmBI	CGTCTC	1 cut(s)	1520
BsmFI	GGGAC	1 cut(s)	2196
BsmI	GAATGC	1 cut(s)	1090
BsnI	GGCC	5 cut(s)	5, 925, 2242, 2337, 2840
BsoBI	CYCGRG	1 cut(s)	2841
Bsp1286I	GDGCHC	3 cut(s)	1878, 2641, 3019
Bsp1407I	TGTACA	2 cut(s)	598, 811
Bsp1720I	GCTNAGC	1 cut(s)	2862
Bsp19I	CCATGG	1 cut(s)	1882
BspACI	CCGC	3 cut(s)	1844, 2709, 3069
BspANI	GGCC	5 cut(s)	5, 925, 2242, 2337, 2840
BspCNI	CTCAG	7 cut(s)	214, 602, 1338, 2098, 2146, 2716, 2788
BspLI	GGNNCC	6 cut(s)	1420, 1612, 1673, 2254, 2331, 3061
BspMAI	CTGCAG	4 cut(s)	370, 1855, 2932, 3068
BspMI	ACCTGC	2 cut(s)	865, 2857
BspPI	GGATC	9 cut(s)	1413, 1426, 1605, 1618, 2166, 2740, 3041, 3158, 3164
BspTI	CTTAAG	1 cut(s)	2114
BsrDI	GCAATG	1 cut(s)	187
BsrGI	TGTACA	2 cut(s)	598, 811
BsrI	ACTGG	2 cut(s)	24, 964
BssECI	CCNNGG	7 cut(s)	945, 1614, 1666, 1744, 1882, 2471, 3035
BssT1I	CCWWGG	2 cut(s)	1614, 1882
Bst2UI	CCWGG	4 cut(s)	947, 1746, 2334, 3037
Bst4CI	ACNGT	5 cut(s)	201, 612, 818, 1583, 1667
Bst6I	CTCTTC	4 cut(s)	1277, 1286, 1632, 2442
BstAFI	CTTAAG	1 cut(s)	2114
BstAUI	TGTACA	2 cut(s)	598, 811
BstC8I	GCNNGC	2 cut(s)	991, 2893
BstDSI	CCRYGG	2 cut(s)	1666, 1882
BstENI	CCTNNNNNAGG	2 cut(s)	2247, 3054
BstF5I	GGATG	5 cut(s)	577, 1415, 1754, 2077, 3079
BstH2I	RGCGCY	1 cut(s)	1659
BstHHI	GCGC	3 cut(s)	149, 1170, 1658
BstMAI	GTCTC	5 cut(s)	1288, 1520, 2009, 2402, 2517
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 1850, 1853, 2348
BstNI	CCWGG	4 cut(s)	947, 1746, 2334, 3037
BstSCI	CCNGG	4 cut(s)	945, 1744, 2332, 3035
BstSFI	CTRYAG	4 cut(s)	366, 1851, 2928, 3064
BstV2I	GAAGAC	2 cut(s)	1193, 1716
BstX2I	RGATCY	7 cut(s)	463, 724, 1418, 1501, 1610, 1801, 2158
BstYI	RGATCY	7 cut(s)	463, 724, 1418, 1501, 1610, 1801, 2158
BsuRI	GGCC	5 cut(s)	5, 925, 2242, 2337, 2840
BtgI	CCRYGG	2 cut(s)	1666, 1882
BtrI	CACGTC	1 cut(s)	2276
BtsCI	GGATG	5 cut(s)	577, 1415, 1754, 2077, 3079
BtsI	GCAGTG	2 cut(s)	375, 1159
BtsIMutI	CAGTG	4 cut(s)	375, 1159, 1588, 2103
BveI	ACCTGC	2 cut(s)	865, 2857
Cac8I	GCNNGC	2 cut(s)	991, 2893
CaiI	CAGNNNCTG	1 cut(s)	2354
CfoI	GCGC	3 cut(s)	149, 1170, 1658
Cfr13I	GGNCC	7 cut(s)	391, 479, 1931, 2240, 2330, 2468, 2839
Csp6I	GTAC	8 cut(s)	75, 381, 599, 632, 812, 854, 2041, 2427
CviAII	CATG	8 cut(s)	92, 385, 418, 596, 759, 1883, 2402, 2570
CviQI	GTAC	8 cut(s)	75, 381, 599, 632, 812, 854, 2041, 2427
DraI	TTTAAA	1 cut(s)	2298
EaeI	YGGCCR	2 cut(s)	3, 2335
Eam1104I	CTCTTC	4 cut(s)	1277, 1286, 1632, 2442
EarI	CTCTTC	4 cut(s)	1277, 1286, 1632, 2442
Ecl136II	GAGCTC	2 cut(s)	2639, 3017
Eco130I	CCWWGG	2 cut(s)	1614, 1882
Eco147I	AGGCCT	1 cut(s)	925
Eco24I	GRGCYC	2 cut(s)	2641, 3019
Eco32I	GATATC	1 cut(s)	317
Eco47I	GGWCC	5 cut(s)	391, 479, 1931, 2330, 2468
Eco53kI	GAGCTC	2 cut(s)	2639, 3017
Eco57I	CTGAAG	8 cut(s)	15, 588, 741, 906, 1457, 1554, 1613, 1860
Eco88I	CYCGRG	1 cut(s)	2841
EcoICRI	GAGCTC	2 cut(s)	2639, 3017
EcoNI	CCTNNNNNAGG	2 cut(s)	2247, 3054
EcoO109I	RGGNCCY	1 cut(s)	2330
EcoRI	GAATTC	2 cut(s)	226, 427
EcoRII	CCWGG	4 cut(s)	945, 1744, 2332, 3035
EcoRV	GATATC	1 cut(s)	317
EcoT14I	CCWWGG	2 cut(s)	1614, 1882
EcoT22I	ATGCAT	2 cut(s)	93, 1996
EcoT38I	GRGCYC	2 cut(s)	2641, 3019
ErhI	CCWWGG	2 cut(s)	1614, 1882
Esp3I	CGTCTC	1 cut(s)	1520
FaeI	CATG	8 cut(s)	95, 388, 421, 599, 762, 1886, 2405, 2573
FalI	AAGNNNNNCTT	4 cut(s)	828, 860, 1450, 1482
FaqI	GGGAC	1 cut(s)	2196
FatI	CATG	8 cut(s)	91, 384, 417, 595, 758, 1882, 2401, 2569
FauI	CCCGC	1 cut(s)	3062
FauNDI	CATATG	1 cut(s)	2413
FbaI	TGATCA	3 cut(s)	439, 2550, 3004
FokI	GGATG	5 cut(s)	564, 1422, 1761, 2084, 3086
FriOI	GRGCYC	2 cut(s)	2641, 3019
GlaI	GCGC	3 cut(s)	148, 1169, 1657
GsaI	CCCAGC	2 cut(s)	900, 1825
GsuI	CTGGAG	1 cut(s)	2079
HaeII	RGCGCY	1 cut(s)	1659
HaeIII	GGCC	5 cut(s)	5, 925, 2242, 2337, 2840
HapII	CCGG	3 cut(s)	1363, 1401, 2256
HhaI	GCGC	3 cut(s)	149, 1170, 1658
Hin1II	CATG	8 cut(s)	95, 388, 421, 599, 762, 1886, 2405, 2573
Hin6I	GCGC	3 cut(s)	147, 1168, 1656
HinP1I	GCGC	3 cut(s)	147, 1168, 1656
HpaII	CCGG	3 cut(s)	1363, 1401, 2256
HphI	GGTGA	7 cut(s)	200, 830, 2505, 2545, 2993, 3098, 3106
Hpy166II	GTNNAC	8 cut(s)	122, 191, 560, 682, 812, 814, 821, 3113
Hpy8I	GTNNAC	8 cut(s)	122, 191, 560, 682, 812, 814, 821, 3113
Hpy99I	CGWCG	2 cut(s)	1727, 1730
HpyCH4III	ACNGT	5 cut(s)	201, 612, 818, 1583, 1667
HpyCH4IV	ACGT	6 cut(s)	77, 264, 1513, 1620, 2275, 2535
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 1850, 1853, 2348
HpySE526I	ACGT	6 cut(s)	77, 264, 1513, 1620, 2275, 2535
Hsp92II	CATG	8 cut(s)	95, 388, 421, 599, 762, 1886, 2405, 2573
HspAI	GCGC	3 cut(s)	147, 1168, 1656
Ksp22I	TGATCA	3 cut(s)	439, 2550, 3004
LweI	GCATC	9 cut(s)	78, 586, 976, 1381, 1865, 2003, 2009, 2080, 3064
MaeII	ACGT	6 cut(s)	77, 264, 1513, 1620, 2275, 2535
MfeI	CAATTG	1 cut(s)	242
MflI	RGATCY	7 cut(s)	463, 724, 1418, 1501, 1610, 1801, 2158
MhlI	GDGCHC	3 cut(s)	1878, 2641, 3019
MlsI	TGGCCA	1 cut(s)	2337
MluNI	TGGCCA	1 cut(s)	2337
MlyI	GAGTC	9 cut(s)	842, 1006, 1341, 1781, 2011, 2144, 2333, 2519, 3091
MmeI	TCCRAC	1 cut(s)	506
Mox20I	TGGCCA	1 cut(s)	2337
Mph1103I	ATGCAT	2 cut(s)	93, 1996
MroXI	GAANNNNTTC	3 cut(s)	1026, 1476, 1713
MscI	TGGCCA	1 cut(s)	2337
MseI	TTAA	8 cut(s)	696, 981, 1205, 1703, 1782, 2115, 2297, 2538
MslI	CAYNNNNRTG	2 cut(s)	867, 1881
Msp20I	TGGCCA	1 cut(s)	2337
MspA1I	CMGCKG	3 cut(s)	365, 1821, 3069
MspCI	CTTAAG	1 cut(s)	2114
MspI	CCGG	3 cut(s)	1363, 1401, 2256
MspR9I	CCNGG	4 cut(s)	947, 1746, 2334, 3037
MunI	CAATTG	1 cut(s)	242
Mva1269I	GAATGC	1 cut(s)	1090
MvaI	CCWGG	4 cut(s)	947, 1746, 2334, 3037
MwoI	GCNNNNNNNGC	4 cut(s)	11, 1850, 1853, 2348
NcoI	CCATGG	1 cut(s)	1882
NdeI	CATATG	1 cut(s)	2413
NlaIII	CATG	8 cut(s)	95, 388, 421, 599, 762, 1886, 2405, 2573
NlaIV	GGNNCC	6 cut(s)	1420, 1612, 1673, 2254, 2331, 3061
NmuCI	GTSAC	8 cut(s)	217, 1039, 1154, 1661, 1751, 2101, 2511, 3094
NsiI	ATGCAT	2 cut(s)	93, 1996
PceI	AGGCCT	1 cut(s)	925
PctI	GAATGC	1 cut(s)	1090
PdmI	GAANNNNTTC	3 cut(s)	1026, 1476, 1713
PleI	GAGTC	9 cut(s)	842, 1005, 1340, 1781, 2011, 2144, 2332, 2519, 3091
PpsI	GAGTC	9 cut(s)	842, 1005, 1340, 1781, 2011, 2144, 2332, 2519, 3091
PpuMI	RGGWCCY	1 cut(s)	2330
Psp124BI	GAGCTC	2 cut(s)	2641, 3019
Psp1406I	AACGTT	1 cut(s)	2535
Psp5II	RGGWCCY	1 cut(s)	2330
Psp6I	CCWGG	4 cut(s)	945, 1744, 2332, 3035
PspFI	CCCAGC	2 cut(s)	896, 1821
PspGI	CCWGG	4 cut(s)	945, 1744, 2332, 3035
PspN4I	GGNNCC	6 cut(s)	1420, 1612, 1673, 2254, 2331, 3061
PspPI	GGNCC	7 cut(s)	391, 479, 1931, 2240, 2330, 2468, 2839
PspPPI	RGGWCCY	1 cut(s)	2330
PstI	CTGCAG	4 cut(s)	370, 1855, 2932, 3068
PstNI	CAGNNNCTG	1 cut(s)	2354
PsuI	RGATCY	7 cut(s)	463, 724, 1418, 1501, 1610, 1801, 2158
PvuII	CAGCTG	2 cut(s)	365, 1821
RsaI	GTAC	8 cut(s)	76, 382, 600, 633, 813, 855, 2042, 2428
RsaNI	GTAC	8 cut(s)	75, 381, 599, 632, 812, 854, 2041, 2427
RseI	CAYNNNNRTG	2 cut(s)	867, 1881
SacI	GAGCTC	2 cut(s)	2641, 3019
SaqAI	TTAA	8 cut(s)	696, 981, 1205, 1703, 1782, 2115, 2297, 2538
Sau96I	GGNCC	7 cut(s)	391, 479, 1931, 2240, 2330, 2468, 2839
ScaI	AGTACT	1 cut(s)	2042
SchI	GAGTC	9 cut(s)	842, 1006, 1341, 1781, 2011, 2144, 2333, 2519, 3091
ScrFI	CCNGG	4 cut(s)	947, 1746, 2334, 3037
SduI	GDGCHC	3 cut(s)	1878, 2641, 3019
SfaNI	GCATC	9 cut(s)	78, 586, 976, 1381, 1865, 2003, 2009, 2080, 3064
SfcI	CTRYAG	4 cut(s)	366, 1851, 2928, 3064
SinI	GGWCC	5 cut(s)	391, 479, 1931, 2330, 2468
SmiMI	CAYNNNNRTG	2 cut(s)	867, 1881
SmlI	CTYRAG	2 cut(s)	1791, 2114
SmoI	CTYRAG	2 cut(s)	1791, 2114
SpeI	ACTAGT	1 cut(s)	2717
SseBI	AGGCCT	1 cut(s)	925
SsiI	CCGC	3 cut(s)	1844, 2709, 3069
SstI	GAGCTC	2 cut(s)	2641, 3019
StuI	AGGCCT	1 cut(s)	925
StyD4I	CCNGG	4 cut(s)	945, 1744, 2332, 3035
StyI	CCWWGG	2 cut(s)	1614, 1882
TaaI	ACNGT	5 cut(s)	201, 612, 818, 1583, 1667
TaiI	ACGT	6 cut(s)	80, 267, 1516, 1623, 2278, 2538
TaqI	TCGA	3 cut(s)	1518, 2644, 2678
TaqII	GACCGA	2 cut(s)	2485, 2832
TatI	WGTACW	3 cut(s)	598, 811, 2040
TauI	GCSGC	1 cut(s)	1847
Tru1I	TTAA	8 cut(s)	696, 981, 1205, 1703, 1782, 2115, 2297, 2538
Tru9I	TTAA	8 cut(s)	696, 981, 1205, 1703, 1782, 2115, 2297, 2538
TscAI	CASTG	4 cut(s)	375, 1159, 1588, 2110
TseFI	GTSAC	8 cut(s)	217, 1039, 1154, 1661, 1751, 2101, 2511, 3094
Tsp45I	GTSAC	8 cut(s)	217, 1039, 1154, 1661, 1751, 2101, 2511, 3094
TspGWI	ACGGA	2 cut(s)	1082, 1135
TspRI	CASTG	4 cut(s)	375, 1159, 1588, 2110
Vha464I	CTTAAG	1 cut(s)	2114
VpaK11BI	GGWCC	5 cut(s)	391, 479, 1931, 2330, 2468
XagI	CCTNNNNNAGG	2 cut(s)	2247, 3054
XapI	RAATTY	5 cut(s)	226, 427, 794, 1048, 1322
XbaI	TCTAGA	2 cut(s)	2270, 2575
XmnI	GAANNNNTTC	3 cut(s)	1026, 1476, 1713
ZrmI	AGTACT	1 cut(s)	2042
Zsp2I	ATGCAT	2 cut(s)	93, 1996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.