Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005406

Physical Location & Seq

Genomic Coordinates Reverse (-)

20196048 .. 20197865

Gene Structure 1818 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1095900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGCCGACCGTATTAGAGCTTTTCTACAGCCGGAGGTCTCGCTACAACCGAGCCGCAACCGATCCAGGGTTCGACTCTCTCCCTCCTCCTCCGACCGTTGACCGAAGCCACAACCACAATCAAAGTCGCCGCCACCACAATTCTGACGGCTGCGATCCCCTCCTCCGCCGAACCCCGCGCCATTTCCGGCATCGTTCCTCCCTCCCTGAGCGGGCGCCGGTTGGGCTCGAGCAGGGCGGGACCCACCCGGGTTCTGGAAATGGGGTCGCCGGAAGCAGTGGCGGGTTGAGCGGGAACGAGAGGCTTCCCGGGTCTGTTTTGCTCGCCAGGGAAAGGCTGCTGGAGAGGCTGAGAGGGATGCCTCCTTCAGAAACCAGGCGGCATAGAGCATTGCATCTTTACGGTGGTGGGCTACTACATGGTGATGACTTGAGTCCTCTTGTAAGGGATTCGGACGCTGAAATCTTGACAGGTCAGCCAGCCAGTGCCTACTCTTCGACTAACTTGGGCTCCCAAATTGAAAGCCTACATCTCTTGCAAGAATCGAACAAGAAGCCTCCAGGTCTTAGTCAAGAGGTCTTTGATTGTTTGAACCTTGAGATTTTCAGTAGCACAGAAATGGGTGTTGAAGGGTTGGAGTTAGGAGCTTCTCAAGATTGTAGTATCTGTTTGGAGAGTTTTATGGATGGCAACAAGCTTATATGCTTACCTTGTGAGCATAGATTCCATGCTGCCTGCTTGAGTCCCTGGGCTCGTATTCGAGGGGACTGCCCGTACTGCCGGAAAGTGATAGTTGTAGATAGTCAAACTGCTAAAAGGAATACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001664 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005109 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007267 GO:0008150 GO:0008152 GO:0008283 GO:0009056 GO:0009057 GO:0009966 GO:0009968 GO:0009987 GO:0010498 GO:0010646 GO:0010648 GO:0016020 GO:0016021 GO:0016055 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022603 GO:0023051 GO:0023052 GO:0023057 GO:0030111 GO:0030163 GO:0030178 GO:0031224 GO:0031226 GO:0032446 GO:0032801 GO:0035567 GO:0036211 GO:0038018 GO:0043112 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044425 GO:0044459 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0060070 GO:0060071 GO:0060828 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0072089 GO:0090090 GO:0090175 GO:0140096 GO:0198738 GO:1901564 GO:1901565 GO:1901575 GO:1905114 GO:1905330 GO:2000026 GO:2000027 GO:2000050 GO:2000051

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

276

Amino Acids

30.2

Weight (kDa)

7.67

Isoelectric Point (pI)

72.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	219 - 261	2.5e-13	Ring finger domain
zf-RING_11	PF17123	219 - 247	1.6e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	220 - 260	6.5e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	220 - 260	8e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005406)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49850
fragaria_vesca	FvH4_2g04490 FvH4_2g04490
malus_domestica	MD05G1095900.v1.1 MD09G1148100.v1.1 MD10G1102100.v1.1
prunus_persica	Prupe.8G140300_v2.0.a1 Prupe.8G140300_v2.0.a1 Prupe.8G140300_v2.0.a1 Prupe.8G140300_v2.0.a1
pyrus_communis	pycom05g09460 pycom10g08780
rosa_chinensis	RchiOBHm_Chr6g0253981
rosa_laevigata	RLG00000014951
rosa_multiflora	Rmu_sc0001716.1_g000017
rosa_roxburghii	Rroxscaffold_7G00211330
rosa_rugosa	Rorug04G0080100 Rorug05G0548700
rosa_samantha	Rh2BG479300 Rh5DG329300 Rh6AG065200 Rh6AG082700 Rh6BG058200 Rh6CG058200 Rh6CG058300 Rh6DG055100 Rh6DG069100 Rh7CG154800
rosa_wichuraiana	Rw6G005740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	214
AccBSI	CCGCTC	2 cut(s)	211, 291
AccII	CGCG	1 cut(s)	178
AciI	CCGC	9 cut(s)	54, 130, 166, 176, 211, 237, 282, 291, 379
AclWI	GGATC	2 cut(s)	56, 149
AcuI	CTGAAG	1 cut(s)	351
AcyI	GRCGYC	1 cut(s)	215
AfaI	GTAC	1 cut(s)	776
AfiI	CCNNNNNNNGG	4 cut(s)	66, 211, 254, 333
AgsI	TTSAA	3 cut(s)	521, 592, 629
AjnI	CCWGG	5 cut(s)	64, 326, 374, 559, 746
AluBI	AGCT	3 cut(s)	19, 647, 697
AluI	AGCT	3 cut(s)	19, 647, 697
Alw26I	GTCTC	1 cut(s)	42
AlwI	GGATC	2 cut(s)	56, 149
Ama87I	CYCGRG	3 cut(s)	227, 247, 308
ApeKI	GCWGC	3 cut(s)	150, 337, 731
AspLEI	GCGC	2 cut(s)	180, 217
AspS9I	GGNCC	1 cut(s)	240
AsuC2I	CCSGG	4 cut(s)	248, 249, 309, 310
AsuHPI	GGTGA	1 cut(s)	434
AvaI	CYCGRG	3 cut(s)	227, 247, 308
AvaII	GGWCC	1 cut(s)	240
BanI	GGYRCC	1 cut(s)	214
BanII	GRGCYC	3 cut(s)	228, 512, 754
BbvI	GCAGC	3 cut(s)	137, 324, 718
BccI	CCATC	1 cut(s)	680
BceAI	ACGGC	1 cut(s)	163
BcgI	CGANNNNNNTGC	2 cut(s)	750, 784
BciT130I	CCWGG	5 cut(s)	66, 328, 376, 561, 748
BcnI	CCSGG	4 cut(s)	248, 249, 309, 310
BcoDI	GTCTC	1 cut(s)	42
BfmI	CTRYAG	1 cut(s)	25
BfoI	RGCGCY	1 cut(s)	218
BglI	GCCNNNNNGGC	1 cut(s)	223
BisI	GCNGC	6 cut(s)	54, 130, 151, 338, 380, 732
BlsI	GCNGC	6 cut(s)	55, 131, 152, 339, 381, 733
Bme1390I	CCNGG	9 cut(s)	66, 248, 249, 309, 310, 328, 376, 561, 748
Bme18I	GGWCC	1 cut(s)	240
BmeT110I	CYCGRG	3 cut(s)	227, 247, 308
BmgT120I	GGNCC	1 cut(s)	240
BmiI	GGNNCC	4 cut(s)	216, 241, 242, 511
BmrFI	CCNGG	9 cut(s)	66, 248, 249, 309, 310, 328, 376, 561, 748
BmsI	GCATC	3 cut(s)	199, 348, 403
BoxI	GACNNNNGTC	1 cut(s)	432
BpmI	CTGGAG	2 cut(s)	362, 543
Bpu10I	CCTNAGC	1 cut(s)	207
BpuEI	CTTGAG	4 cut(s)	451, 617, 636, 760
BpuMI	CCSGG	4 cut(s)	248, 249, 309, 310
BsaHI	GRCGYC	1 cut(s)	215
BsaI	GGTCTC	1 cut(s)	42
BsaJI	CCNNGG	6 cut(s)	65, 247, 308, 327, 746, 747
BsaXI	ACNNNNNCTCC	2 cut(s)	494, 524
Bsc4I	CCNNNNNNNGG	4 cut(s)	66, 211, 254, 333
Bse118I	RCCGGY	1 cut(s)	217
Bse1I	ACTGG	1 cut(s)	483
Bse3DI	GCAATG	1 cut(s)	389
BseBI	CCWGG	5 cut(s)	66, 328, 376, 561, 748
BseDI	CCNNGG	6 cut(s)	65, 247, 308, 327, 746, 747
BseGI	GGATG	2 cut(s)	363, 691
BseLI	CCNNNNNNNGG	4 cut(s)	66, 211, 254, 333
BseMI	GCAATG	1 cut(s)	389
BseMII	CTCAG	2 cut(s)	198, 341
BseNI	ACTGG	1 cut(s)	483
BseRI	GAGGAG	3 cut(s)	75, 78, 152
BseXI	GCAGC	3 cut(s)	137, 324, 718
Bsh1236I	CGCG	1 cut(s)	178
Bsh1285I	CGRYCG	2 cut(s)	9, 96
BshNI	GGYRCC	1 cut(s)	214
BsiEI	CGRYCG	2 cut(s)	9, 96
BsiHKCI	CYCGRG	3 cut(s)	227, 247, 308
BsiSI	CCGG	7 cut(s)	31, 187, 218, 248, 270, 309, 781
BslFI	GGGAC	3 cut(s)	253, 729, 779
BslI	CCNNNNNNNGG	4 cut(s)	66, 211, 254, 333
BsmAI	GTCTC	1 cut(s)	42
BsmFI	GGGAC	3 cut(s)	253, 729, 779
Bso31I	GGTCTC	1 cut(s)	42
BsoBI	CYCGRG	3 cut(s)	227, 247, 308
Bsp1286I	GDGCHC	3 cut(s)	228, 512, 754
Bsp143I	GATC	2 cut(s)	61, 154
BspACI	CCGC	9 cut(s)	54, 130, 166, 176, 211, 237, 282, 291, 379
BspCNI	CTCAG	2 cut(s)	199, 342
BspFNI	CGCG	1 cut(s)	178
BspLI	GGNNCC	4 cut(s)	216, 241, 242, 511
BspPI	GGATC	2 cut(s)	56, 149
BspT107I	GGYRCC	1 cut(s)	214
BspTNI	GGTCTC	1 cut(s)	42
BsrBI	CCGCTC	2 cut(s)	211, 291
BsrDI	GCAATG	1 cut(s)	389
BsrFI	RCCGGY	1 cut(s)	217
BsrI	ACTGG	1 cut(s)	483
BssAI	RCCGGY	1 cut(s)	217
BssECI	CCNNGG	6 cut(s)	65, 247, 308, 327, 746, 747
BssMI	GATC	2 cut(s)	61, 154
BssNI	GRCGYC	1 cut(s)	215
Bst2UI	CCWGG	5 cut(s)	66, 328, 376, 561, 748
Bst4CI	ACNGT	3 cut(s)	10, 97, 404
Bst6I	CTCTTC	1 cut(s)	499
BstACI	GRCGYC	1 cut(s)	215
BstC8I	GCNNGC	4 cut(s)	213, 324, 480, 736
BstDEI	CTNAG	3 cut(s)	207, 350, 566
BstF5I	GGATG	2 cut(s)	363, 691
BstFNI	CGCG	1 cut(s)	178
BstH2I	RGCGCY	1 cut(s)	218
BstHHI	GCGC	2 cut(s)	180, 217
BstKTI	GATC	2 cut(s)	64, 157
BstMAI	GTCTC	1 cut(s)	42
BstMBI	GATC	2 cut(s)	61, 154
BstMCI	CGRYCG	2 cut(s)	9, 96
BstMWI	GCNNNNNNNGC	4 cut(s)	223, 288, 346, 777
BstNI	CCWGG	5 cut(s)	66, 328, 376, 561, 748
BstPAI	GACNNNNGTC	1 cut(s)	432
BstSCI	CCNGG	9 cut(s)	64, 246, 247, 307, 308, 326, 374, 559, 746
BstSFI	CTRYAG	1 cut(s)	25
BstUI	CGCG	1 cut(s)	178
BstV1I	GCAGC	3 cut(s)	137, 324, 718
BtsCI	GGATG	2 cut(s)	363, 691
BtsI	GCAGTG	1 cut(s)	283
BtsIMutI	CAGTG	2 cut(s)	283, 490
Cac8I	GCNNGC	4 cut(s)	213, 324, 480, 736
CfoI	GCGC	2 cut(s)	180, 217
Cfr10I	RCCGGY	1 cut(s)	217
Cfr13I	GGNCC	1 cut(s)	240
Cfr9I	CCCGGG	2 cut(s)	247, 308
CseI	GACGC	1 cut(s)	464
Csp6I	GTAC	1 cut(s)	775
CviAII	CATG	2 cut(s)	419, 728
CviQI	GTAC	1 cut(s)	775
DdeI	CTNAG	3 cut(s)	207, 350, 566
DinI	GGCGCC	1 cut(s)	216
DpnI	GATC	2 cut(s)	63, 156
DpnII	GATC	2 cut(s)	61, 154
Eam1104I	CTCTTC	1 cut(s)	499
EarI	CTCTTC	1 cut(s)	499
EciI	GGCGGA	1 cut(s)	155
Eco24I	GRGCYC	3 cut(s)	228, 512, 754
Eco31I	GGTCTC	1 cut(s)	42
Eco47I	GGWCC	1 cut(s)	240
Eco57I	CTGAAG	1 cut(s)	351
Eco88I	CYCGRG	3 cut(s)	227, 247, 308
EcoO109I	RGGNCCY	1 cut(s)	240
EcoRII	CCWGG	5 cut(s)	64, 326, 374, 559, 746
EcoT38I	GRGCYC	3 cut(s)	228, 512, 754
EgeI	GGCGCC	1 cut(s)	216
EheI	GGCGCC	1 cut(s)	216
FaeI	CATG	2 cut(s)	422, 731
FaiI	YATR	8 cut(s)	384, 420, 683, 701, 703, 720, 729, 826
FaqI	GGGAC	3 cut(s)	253, 729, 779
FatI	CATG	2 cut(s)	418, 727
FauI	CCCGC	5 cut(s)	183, 204, 230, 275, 284
Fnu4HI	GCNGC	6 cut(s)	54, 130, 151, 338, 380, 732
FokI	GGATG	2 cut(s)	370, 698
FriOI	GRGCYC	3 cut(s)	228, 512, 754
Fsp4HI	GCNGC	6 cut(s)	54, 130, 151, 338, 380, 732
GlaI	GCGC	2 cut(s)	179, 216
GluI	GCNGC	6 cut(s)	54, 130, 151, 338, 380, 732
GsuI	CTGGAG	2 cut(s)	362, 543
HaeII	RGCGCY	1 cut(s)	218
HapII	CCGG	7 cut(s)	31, 187, 218, 248, 270, 309, 781
HgaI	GACGC	1 cut(s)	464
HhaI	GCGC	2 cut(s)	180, 217
Hin1I	GRCGYC	1 cut(s)	215
Hin1II	CATG	2 cut(s)	422, 731
Hin6I	GCGC	2 cut(s)	178, 215
HinP1I	GCGC	2 cut(s)	178, 215
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HindIII	AAGCTT	1 cut(s)	695
HinfI	GANTC	6 cut(s)	74, 433, 449, 542, 723, 742
HpaII	CCGG	7 cut(s)	31, 187, 218, 248, 270, 309, 781
HphI	GGTGA	1 cut(s)	434
Hpy166II	GTNNAC	1 cut(s)	100
Hpy188I	TCNGA	4 cut(s)	93, 145, 370, 454
Hpy188III	TCNNGA	4 cut(s)	255, 466, 572, 653
Hpy8I	GTNNAC	1 cut(s)	100
HpyAV	CCTTC	2 cut(s)	375, 623
HpyCH4III	ACNGT	3 cut(s)	10, 97, 404
HpyCH4V	TGCA	2 cut(s)	394, 538
HpyF10VI	GCNNNNNNNGC	4 cut(s)	223, 288, 346, 777
HpyF3I	CTNAG	3 cut(s)	207, 350, 566
Hsp92I	GRCGYC	1 cut(s)	215
Hsp92II	CATG	2 cut(s)	422, 731
HspAI	GCGC	2 cut(s)	178, 215
KasI	GGCGCC	1 cut(s)	214
KflI	GGGWCCC	1 cut(s)	240
Kzo9I	GATC	2 cut(s)	61, 154
LmnI	GCTCC	2 cut(s)	515, 644
Lsp1109I	GCAGC	3 cut(s)	137, 324, 718
LweI	GCATC	3 cut(s)	199, 348, 403
MalI	GATC	2 cut(s)	63, 156
MbiI	CCGCTC	2 cut(s)	211, 291
MboI	GATC	2 cut(s)	61, 154
MboII	GAAGA	1 cut(s)	486
MhlI	GDGCHC	3 cut(s)	228, 512, 754
MluCI	AATT	2 cut(s)	139, 516
Mly113I	GGCGCC	1 cut(s)	215
MlyI	GAGTC	3 cut(s)	68, 442, 751
MmeI	TCCRAC	2 cut(s)	116, 615
MslI	CAYNNNNRTG	2 cut(s)	423, 617
MspI	CCGG	7 cut(s)	31, 187, 218, 248, 270, 309, 781
MspR9I	CCNGG	9 cut(s)	66, 248, 249, 309, 310, 328, 376, 561, 748
MvaI	CCWGG	5 cut(s)	66, 328, 376, 561, 748
MvnI	CGCG	1 cut(s)	178
MwoI	GCNNNNNNNGC	4 cut(s)	223, 288, 346, 777
NarI	GGCGCC	1 cut(s)	215
NciI	CCSGG	4 cut(s)	248, 249, 309, 310
NdeII	GATC	2 cut(s)	61, 154
NlaIII	CATG	2 cut(s)	422, 731
NlaIV	GGNNCC	4 cut(s)	216, 241, 242, 511
PaeR7I	CTCGAG	1 cut(s)	227
PasI	CCCWGGG	1 cut(s)	747
PcsI	WCGNNNNNNNCGW	1 cut(s)	46
PfeI	GAWTC	3 cut(s)	449, 542, 723
PkrI	GCNGC	6 cut(s)	55, 131, 152, 339, 381, 733
PleI	GAGTC	3 cut(s)	68, 441, 750
PluTI	GGCGCC	1 cut(s)	218
PpsI	GAGTC	3 cut(s)	68, 441, 750
PpuMI	RGGWCCY	1 cut(s)	240
PshAI	GACNNNNGTC	1 cut(s)	432
Psp5II	RGGWCCY	1 cut(s)	240
Psp6I	CCWGG	5 cut(s)	64, 326, 374, 559, 746
PspGI	CCWGG	5 cut(s)	64, 326, 374, 559, 746
PspN4I	GGNNCC	4 cut(s)	216, 241, 242, 511
PspPI	GGNCC	1 cut(s)	240
PspPPI	RGGWCCY	1 cut(s)	240
PspXI	VCTCGAGB	1 cut(s)	227
RsaI	GTAC	1 cut(s)	776
RsaNI	GTAC	1 cut(s)	775
RseI	CAYNNNNRTG	2 cut(s)	423, 617
SatI	GCNGC	6 cut(s)	54, 130, 151, 338, 380, 732
Sau3AI	GATC	2 cut(s)	61, 154
Sau96I	GGNCC	1 cut(s)	240
SchI	GAGTC	3 cut(s)	68, 442, 751
ScrFI	CCNGG	9 cut(s)	66, 248, 249, 309, 310, 328, 376, 561, 748
SduI	GDGCHC	3 cut(s)	228, 512, 754
SetI	ASST	9 cut(s)	21, 38, 475, 565, 579, 597, 649, 699, 712
SfaNI	GCATC	3 cut(s)	199, 348, 403
SfcI	CTRYAG	1 cut(s)	25
SfoI	GGCGCC	1 cut(s)	216
Sfr274I	CTCGAG	1 cut(s)	227
SinI	GGWCC	1 cut(s)	240
SlaI	CTCGAG	1 cut(s)	227
SmaI	CCCGGG	2 cut(s)	249, 310
SmiMI	CAYNNNNRTG	2 cut(s)	423, 617
SmlI	CTYRAG	5 cut(s)	227, 430, 596, 651, 739
SmoI	CTYRAG	5 cut(s)	227, 430, 596, 651, 739
Sse9I	AATT	2 cut(s)	139, 516
SsiI	CCGC	9 cut(s)	54, 130, 166, 176, 211, 237, 282, 291, 379
SspDI	GGCGCC	1 cut(s)	214
StyD4I	CCNGG	9 cut(s)	64, 246, 247, 307, 308, 326, 374, 559, 746
TaaI	ACNGT	3 cut(s)	10, 97, 404
TaqI	TCGA	5 cut(s)	72, 228, 497, 545, 760
TaqII	GACCGA	1 cut(s)	117
TasI	AATT	2 cut(s)	139, 516
TauI	GCSGC	3 cut(s)	56, 132, 382
TfiI	GAWTC	3 cut(s)	449, 542, 723
TscAI	CASTG	2 cut(s)	283, 490
TseI	GCWGC	3 cut(s)	150, 337, 731
TspMI	CCCGGG	2 cut(s)	247, 308
TspRI	CASTG	2 cut(s)	283, 490
VpaK11BI	GGWCC	1 cut(s)	240
XcmI	CCANNNNNNNNNTGG	1 cut(s)	251
XhoI	CTCGAG	1 cut(s)	227
XmaI	CCCGGG	2 cut(s)	247, 308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.