Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006471

Physical Location & Seq

Genomic Coordinates Forward (+)

33538344 .. 33542221

Gene Structure 3878 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1206300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 945 bp

ATGGCTGCTTCTTCCTCTCTTAAATTTGCAGCTGGGTTTCTCCAACTGAAGAAACCACAAAACCCATTGCTCCTCCGCTCTCCCAATCAAGTATCCACCCTTTCGACCAAGAAGTTTGCTCCAATGGCGGCTCTCACCGCCACCCCAGCCGTCAGCCTCTCCCAGACTTTCATAAATCTGAAGGAGCAGGGAAAGGTTGCATTTATCCCTTACATCACTGCCGGTGATCCTGATCTTTCAATCACGGCAGAAGCATTGAAGGTGCTTGATTCTTGTGGATCAGACATAATCGAATTAGGTGTTCCATACTCGGATCCATTAGCAGATGGTCCAGTTATACAGGCTGCAGCCACCCGTTCCCTGGCAAGAGGGACCAATCTCAATGCAATTATTTCAATGTTGAAGGAGGTGATTCCTCAATTGTCTTGCCCAATTGCGCTGTTTGCTTACTATAACCCAATTTTGAAGCGTGGAATTCCGGAGTTCATGTCCACCATCAAAGACGTTGGTGTACATGGGCTTGTGGTTCCAGATGTTCCATTGGAAGAAACAGAAATTTTGAGAAAGGAAGCTGTGAAAAATAATATTGAATTGGTATTACTAACTACACCCACTACTCCAATTGATCGCATGAAGGCCATTGCCGAAGCTTCAGAAGGATTTCTGTACCTAGTAAGCTCCATCGGAGTTACTGGTGCCCGTGCATCTGTGAACGAACGAGTTCCTGCCCTTTTAAAGGAAATTAAAGAGACAACAAAAAAGCCTGTAGCAGTTGGTTTCGGCATCTCAAAGCCCGAACATGCGAAACAGGTAGCAGGGTGGGGAGCTGATGGCGTTATCGTTGGTAGTGCGATGGTGAAGCTGTTGGGCGAAGCGAAAACGCCCGAGGAAGGGTTGAAGGAAATAGCAACTTTCACCAAGTCTTTAAAATCTGCACTCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004834 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009606 GO:0009607 GO:0009617 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0016043 GO:0016053 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0019752 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042401 GO:0042430 GO:0042435 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046219 GO:0046394 GO:0046483 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0098542 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00400 ko00402 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map00402 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

Trp_syntA

Protein Analysis

315

Amino Acids

33.27

Weight (kDa)

8.68

Isoelectric Point (pI)

34.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trp_syntA	PF00290	57 - 312	7e-94	Tryptophan synthase alpha chain
His_biosynth	PF00977	230 - 287	6e-06	Histidine biosynthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54640
fragaria_vesca	FvH4_2g22570
malus_domestica	MD05G1206300.v1.1 MD10G1193300.v1.1
prunus_persica	Prupe.8G232700_v2.0.a1
pyrus_communis	pycom05g19230 pycom10g16710
rosa_chinensis	RchiOBHm_Chr3g0488871 RchiOBHm_Chr6g0284041 RchiOBHm_Chr6g0289611
rosa_laevigata	RLG00000012288 RLG00000026432
rosa_multiflora	Rmu_co8332575.1_g000001 Rmu_sc0000607.1_g000042 Rmu_sc0001527.1_g000067 Rmu_sc0001850.1_g000026 Rmu_sc0005376.1_g000002
rosa_roxburghii	Rroxscaffold_1G00026080 Rroxscaffold_7G00177810
rosa_rugosa	Rorug06G0205100
rosa_samantha	Rh5AG506400 Rh6BG323300 Rh6CG329700 Rh6DG267800 Rh6DG315100
rosa_wichuraiana	Rw6G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	695
AccBSI	CCGCTC	1 cut(s)	78
AccIII	TCCGGA	1 cut(s)	478
AciI	CCGC	3 cut(s)	76, 128, 138
AclWI	GGATC	4 cut(s)	221, 286, 308, 321
AcsI	RAATTY	3 cut(s)	23, 474, 555
AcuI	CTGAAG	3 cut(s)	68, 200, 636
AfaI	GTAC	2 cut(s)	513, 668
AfiI	CCNNNNNNNGG	4 cut(s)	361, 736, 890, 891
AgsI	TTSAA	7 cut(s)	240, 259, 396, 403, 466, 590, 898
AjnI	CCWGG	1 cut(s)	360
AluBI	AGCT	6 cut(s)	32, 572, 650, 678, 827, 862
AluI	AGCT	6 cut(s)	32, 572, 650, 678, 827, 862
Alw26I	GTCTC	1 cut(s)	743
AlwI	GGATC	4 cut(s)	221, 286, 308, 321
Ama87I	CYCGRG	1 cut(s)	884
Aor13HI	TCCGGA	1 cut(s)	478
AoxI	GGCC	1 cut(s)	636
ApeKI	GCWGC	4 cut(s)	5, 29, 344, 347
ApoI	RAATTY	3 cut(s)	23, 474, 555
Asp700I	GAANNNNTTC	2 cut(s)	660, 720
AspLEI	GCGC	1 cut(s)	439
AspS9I	GGNCC	2 cut(s)	329, 372
AsuHPI	GGTGA	5 cut(s)	127, 236, 421, 868, 907
AvaI	CYCGRG	1 cut(s)	884
AvaII	GGWCC	2 cut(s)	329, 372
BaeGI	GKGCMC	1 cut(s)	700
BamHI	GGATCC	1 cut(s)	313
BanI	GGYRCC	1 cut(s)	695
BbvI	GCAGC	3 cut(s)	41, 331, 359
BccI	CCATC	5 cut(s)	320, 503, 689, 824, 847
BceAI	ACGGC	2 cut(s)	134, 261
BciT130I	CCWGG	1 cut(s)	362
BciVI	GTATCC	1 cut(s)	103
BcoDI	GTCTC	1 cut(s)	743
BfaI	CTAG	1 cut(s)	671
BfmI	CTRYAG	2 cut(s)	345, 765
BfuI	GTATCC	1 cut(s)	103
BisI	GCNGC	5 cut(s)	6, 30, 129, 345, 348
BlsI	GCNGC	5 cut(s)	7, 31, 130, 346, 349
Bme1390I	CCNGG	1 cut(s)	362
Bme18I	GGWCC	2 cut(s)	329, 372
BmeT110I	CYCGRG	1 cut(s)	884
BmgT120I	GGNCC	2 cut(s)	329, 372
BmiI	GGNNCC	4 cut(s)	315, 373, 528, 697
BmrFI	CCNGG	1 cut(s)	362
BmsI	GCATC	2 cut(s)	713, 792
BsaBI	GATNNNNATC	1 cut(s)	231
BsaJI	CCNNGG	2 cut(s)	360, 885
BsaWI	WCCGGW	1 cut(s)	478
BsaXI	ACNNNNNCTCC	2 cut(s)	473, 503
Bsc4I	CCNNNNNNNGG	4 cut(s)	361, 736, 890, 891
Bse118I	RCCGGY	1 cut(s)	221
Bse1I	ACTGG	2 cut(s)	332, 697
Bse3DI	GCAATG	2 cut(s)	65, 639
Bse8I	GATNNNNATC	1 cut(s)	231
BseAI	TCCGGA	1 cut(s)	478
BseBI	CCWGG	1 cut(s)	362
BseDI	CCNNGG	2 cut(s)	360, 885
BseJI	GATNNNNATC	1 cut(s)	231
BseLI	CCNNNNNNNGG	4 cut(s)	361, 736, 890, 891
BseMI	GCAATG	2 cut(s)	65, 639
BseNI	ACTGG	2 cut(s)	332, 697
BseRI	GAGGAG	2 cut(s)	62, 929
BseSI	GKGCMC	1 cut(s)	700
BseXI	GCAGC	3 cut(s)	41, 331, 359
BseYI	CCCAGC	2 cut(s)	32, 145
BsgI	GTGCAG	1 cut(s)	918
BshFI	GGCC	1 cut(s)	638
BshNI	GGYRCC	1 cut(s)	695
BsiHKCI	CYCGRG	1 cut(s)	884
BsiSI	CCGG	2 cut(s)	222, 479
BslFI	GGGAC	1 cut(s)	385
BslI	CCNNNNNNNGG	4 cut(s)	361, 736, 890, 891
BsmAI	GTCTC	1 cut(s)	743
BsmFI	GGGAC	1 cut(s)	385
BsnI	GGCC	1 cut(s)	638
BsoBI	CYCGRG	1 cut(s)	884
Bsp1286I	GDGCHC	1 cut(s)	700
Bsp13I	TCCGGA	1 cut(s)	478
Bsp1407I	TGTACA	1 cut(s)	511
Bsp143I	GATC	5 cut(s)	226, 232, 278, 313, 625
BspACI	CCGC	3 cut(s)	76, 128, 138
BspANI	GGCC	1 cut(s)	638
BspEI	TCCGGA	1 cut(s)	478
BspLI	GGNNCC	4 cut(s)	315, 373, 528, 697
BspMAI	CTGCAG	1 cut(s)	349
BspPI	GGATC	4 cut(s)	221, 286, 308, 321
BspT107I	GGYRCC	1 cut(s)	695
BsrBI	CCGCTC	1 cut(s)	78
BsrDI	GCAATG	2 cut(s)	65, 639
BsrFI	RCCGGY	1 cut(s)	221
BsrGI	TGTACA	1 cut(s)	511
BsrI	ACTGG	2 cut(s)	332, 697
BssAI	RCCGGY	1 cut(s)	221
BssECI	CCNNGG	2 cut(s)	360, 885
BssMI	GATC	5 cut(s)	226, 232, 278, 313, 625
Bst2UI	CCWGG	1 cut(s)	362
BstAUI	TGTACA	1 cut(s)	511
BstENI	CCTNNNNNAGG	1 cut(s)	734
BstHHI	GCGC	1 cut(s)	439
BstKTI	GATC	5 cut(s)	229, 235, 281, 316, 628
BstMAI	GTCTC	1 cut(s)	743
BstMBI	GATC	5 cut(s)	226, 232, 278, 313, 625
BstMWI	GCNNNNNNNGC	4 cut(s)	125, 137, 146, 443
BstNI	CCWGG	1 cut(s)	362
BstNSI	RCATGY	1 cut(s)	803
BstSCI	CCNGG	1 cut(s)	360
BstSFI	CTRYAG	2 cut(s)	345, 765
BstSLI	GKGCMC	1 cut(s)	700
BstV1I	GCAGC	3 cut(s)	41, 331, 359
BstX2I	RGATCY	1 cut(s)	313
BstYI	RGATCY	1 cut(s)	313
BsuI	GTATCC	1 cut(s)	103
BsuRI	GGCC	1 cut(s)	638
BtgZI	GCGATG	1 cut(s)	866
BtsI	GCAGTG	1 cut(s)	216
BtsIMutI	CAGTG	1 cut(s)	216
CfoI	GCGC	1 cut(s)	439
Cfr10I	RCCGGY	1 cut(s)	221
Cfr13I	GGNCC	2 cut(s)	329, 372
Csp6I	GTAC	2 cut(s)	512, 667
CviAII	CATG	4 cut(s)	487, 515, 631, 800
CviQI	GTAC	2 cut(s)	512, 667
DpnI	GATC	5 cut(s)	228, 234, 280, 315, 627
DpnII	GATC	5 cut(s)	226, 232, 278, 313, 625
DraI	TTTAAA	2 cut(s)	735, 927
Eco47I	GGWCC	2 cut(s)	329, 372
Eco57I	CTGAAG	3 cut(s)	68, 200, 636
Eco88I	CYCGRG	1 cut(s)	884
EcoNI	CCTNNNNNAGG	1 cut(s)	734
EcoRI	GAATTC	1 cut(s)	474
EcoRII	CCWGG	1 cut(s)	360
FaeI	CATG	4 cut(s)	490, 518, 634, 803
FaiI	YATR	9 cut(s)	173, 287, 307, 338, 453, 488, 516, 632, 801
FaqI	GGGAC	1 cut(s)	385
FatI	CATG	4 cut(s)	486, 514, 630, 799
Fnu4HI	GCNGC	5 cut(s)	6, 30, 129, 345, 348
Fsp4HI	GCNGC	5 cut(s)	6, 30, 129, 345, 348
FspBI	CTAG	1 cut(s)	671
GlaI	GCGC	1 cut(s)	438
GluI	GCNGC	5 cut(s)	6, 30, 129, 345, 348
GsaI	CCCAGC	2 cut(s)	36, 149
HaeIII	GGCC	1 cut(s)	638
HapII	CCGG	2 cut(s)	222, 479
HhaI	GCGC	1 cut(s)	439
Hin1II	CATG	4 cut(s)	490, 518, 634, 803
Hin6I	GCGC	1 cut(s)	437
HinP1I	GCGC	1 cut(s)	437
HindIII	AAGCTT	1 cut(s)	648
HinfI	GANTC	2 cut(s)	269, 412
HpaII	CCGG	2 cut(s)	222, 479
HphI	GGTGA	5 cut(s)	127, 236, 421, 868, 907
Hpy166II	GTNNAC	3 cut(s)	492, 512, 712
Hpy188I	TCNGA	6 cut(s)	180, 283, 313, 655, 686, 944
Hpy188III	TCNNGA	3 cut(s)	230, 479, 530
Hpy8I	GTNNAC	3 cut(s)	492, 512, 712
HpyAV	CCTTC	7 cut(s)	175, 253, 397, 628, 650, 884, 892
HpyCH4IV	ACGT	1 cut(s)	504
HpyCH4V	TGCA	6 cut(s)	29, 200, 347, 386, 704, 935
HpyF10VI	GCNNNNNNNGC	4 cut(s)	125, 137, 146, 443
HpySE526I	ACGT	1 cut(s)	504
Hsp92II	CATG	4 cut(s)	490, 518, 634, 803
HspAI	GCGC	1 cut(s)	437
Kpn2I	TCCGGA	1 cut(s)	478
Kzo9I	GATC	5 cut(s)	226, 232, 278, 313, 625
LmnI	GCTCC	5 cut(s)	75, 124, 184, 683, 824
Lsp1109I	GCAGC	3 cut(s)	41, 331, 359
LweI	GCATC	2 cut(s)	713, 792
MaeI	CTAG	1 cut(s)	671
MaeII	ACGT	1 cut(s)	504
MaeIII	GTNAC	1 cut(s)	688
MalI	GATC	5 cut(s)	228, 234, 280, 315, 627
MbiI	CCGCTC	1 cut(s)	78
MboI	GATC	5 cut(s)	226, 232, 278, 313, 625
MboII	GAAGA	3 cut(s)	3, 61, 557
MfeI	CAATTG	3 cut(s)	419, 432, 621
MflI	RGATCY	1 cut(s)	313
MhlI	GDGCHC	1 cut(s)	700
MmeI	TCCRAC	1 cut(s)	67
MnlI	CCTC	7 cut(s)	25, 83, 167, 362, 400, 426, 880
MroI	TCCGGA	1 cut(s)	478
MroXI	GAANNNNTTC	2 cut(s)	660, 720
MseI	TTAA	4 cut(s)	21, 734, 744, 926
MspA1I	CMGCKG	1 cut(s)	32
MspI	CCGG	2 cut(s)	222, 479
MspR9I	CCNGG	1 cut(s)	362
MunI	CAATTG	3 cut(s)	419, 432, 621
MvaI	CCWGG	1 cut(s)	362
MwoI	GCNNNNNNNGC	4 cut(s)	125, 137, 146, 443
NdeII	GATC	5 cut(s)	226, 232, 278, 313, 625
NlaIII	CATG	4 cut(s)	490, 518, 634, 803
NlaIV	GGNNCC	4 cut(s)	315, 373, 528, 697
NspI	RCATGY	1 cut(s)	803
PdmI	GAANNNNTTC	2 cut(s)	660, 720
PfeI	GAWTC	2 cut(s)	269, 412
PkrI	GCNGC	5 cut(s)	7, 31, 130, 346, 349
Psp6I	CCWGG	1 cut(s)	360
PspFI	CCCAGC	2 cut(s)	32, 145
PspGI	CCWGG	1 cut(s)	360
PspN4I	GGNNCC	4 cut(s)	315, 373, 528, 697
PspPI	GGNCC	2 cut(s)	329, 372
PstI	CTGCAG	1 cut(s)	349
PsuI	RGATCY	1 cut(s)	313
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	2 cut(s)	513, 668
RsaNI	GTAC	2 cut(s)	512, 667
SaqAI	TTAA	4 cut(s)	21, 734, 744, 926
SatI	GCNGC	5 cut(s)	6, 30, 129, 345, 348
Sau3AI	GATC	5 cut(s)	226, 232, 278, 313, 625
Sau96I	GGNCC	2 cut(s)	329, 372
ScrFI	CCNGG	1 cut(s)	362
SduI	GDGCHC	1 cut(s)	700
SfaNI	GCATC	2 cut(s)	713, 792
SfcI	CTRYAG	2 cut(s)	345, 765
SinI	GGWCC	2 cut(s)	329, 372
SsiI	CCGC	3 cut(s)	76, 128, 138
SspI	AATATT	1 cut(s)	586
SspMI	CTAG	1 cut(s)	671
StyD4I	CCNGG	1 cut(s)	360
TaiI	ACGT	1 cut(s)	507
TaqI	TCGA	2 cut(s)	104, 291
TatI	WGTACW	1 cut(s)	511
TauI	GCSGC	1 cut(s)	131
TfiI	GAWTC	2 cut(s)	269, 412
Tru1I	TTAA	4 cut(s)	21, 734, 744, 926
Tru9I	TTAA	4 cut(s)	21, 734, 744, 926
TscAI	CASTG	1 cut(s)	223
TseI	GCWGC	4 cut(s)	5, 29, 344, 347
TspDTI	ATGAA	3 cut(s)	160, 475, 647
TspRI	CASTG	1 cut(s)	223
VpaK11BI	GGWCC	2 cut(s)	329, 372
XagI	CCTNNNNNAGG	1 cut(s)	734
XapI	RAATTY	3 cut(s)	23, 474, 555
XceI	RCATGY	1 cut(s)	803
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmnI	GAANNNNTTC	2 cut(s)	660, 720
XspI	CTAG	1 cut(s)	671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.