Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006471

Physical Location & Seq

Genomic Coordinates Forward (+)

28947298 .. 28950789

Gene Structure 3492 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1193300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 945 bp

ATGGCGGCTGCTTCTTCTCTGAAATCTGCAGCTGGGTTTCTCCAACTGAAGAAACCACAAAATCCTTTGCTTCTTAGCTCTCCCAATCAAGTATCCACCATTTCGACCAAGAAGTTTGCTCCAATAGCGGCTCTCACCGCCACCCCAGTCGTCAGCCTCTCCCAGACTTTCATAAATTTGAAGGATCAGGGAAAGGTTGCATTTATCCCTTACATCACTGCTGGTGATCCTGACCTTTCAATCACGGCAGAAGCATTGAAGGTGCTTGATTCTTGTGGATCGGACATAATCGAATTAGGCGTGCCGTATTCTGATCCATTAGCAGATGGTCCAGTTATACAGGCTGCAGCCACCCGTTCCTTGGCAAGAGGGACCAATCTCAATGCAATTATTTCAATGTTGAAGGAGGTGATTCCCCAATTGTCTTGCCCAATTGCTCTGTTTACATACTATAACCCGATTTTGAAGCGTGGGATTCCAGAGTTCATGTCCACCATCAAAGATGTTGGTGTACATGGGCTTGTGGTTCCAGATGTTCCATTGGAGGAAACAGAAATTTTAAGAAAGGAAGCTGCGAAAAATAATATTGAATTGGTATTACTAACAACACCCACTACTCCAATTGATCGAATGAAGGCCATTGCCGAAGCTTCAGAAGGATTTCTGTACCTGGTGAGCTCGATCGGAGTTACTGGTGCCCGTGCATCTGTGAATGAACGAGTTCCTGCTCTTTTAAAGGAAATTAAAGAGACAACAAAAAGGCCTGTTGCGGTTGGTTTCGGCATCTCAAAGCCCGAACACGCGAAACAGGTAGCCGGGTGGGGAGCCGACGGTGTTATCGTTGGTAGTGCGATGGTGAAGCTGTTGGGCGAAGCGAAAACTCCCGAGGAGGGGTTGAAGGAACTAGCAACCTTCACCAAGTCTTTGAAATCTGCCCTCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004834 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009606 GO:0009607 GO:0009617 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0016043 GO:0016053 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0019752 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042401 GO:0042430 GO:0042435 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046219 GO:0046394 GO:0046483 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0098542 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00400 ko00402 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map00402 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

Trp_syntA

Protein Analysis

315

Amino Acids

33.15

Weight (kDa)

8.37

Isoelectric Point (pI)

33.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trp_syntA	PF00290	57 - 312	2.2e-94	Tryptophan synthase alpha chain
His_biosynth	PF00977	230 - 287	9.3e-06	Histidine biosynthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54640
fragaria_vesca	FvH4_2g22570
malus_domestica	MD05G1206300.v1.1 MD10G1193300.v1.1
prunus_persica	Prupe.8G232700_v2.0.a1
pyrus_communis	pycom05g19230 pycom10g16710
rosa_chinensis	RchiOBHm_Chr3g0488871 RchiOBHm_Chr6g0284041 RchiOBHm_Chr6g0289611
rosa_laevigata	RLG00000012288 RLG00000026432
rosa_multiflora	Rmu_co8332575.1_g000001 Rmu_sc0000607.1_g000042 Rmu_sc0001527.1_g000067 Rmu_sc0001850.1_g000026 Rmu_sc0005376.1_g000002
rosa_roxburghii	Rroxscaffold_1G00026080 Rroxscaffold_7G00177810
rosa_rugosa	Rorug06G0205100
rosa_samantha	Rh5AG506400 Rh6BG323300 Rh6CG329700 Rh6DG267800 Rh6DG315100
rosa_wichuraiana	Rw6G027310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	695
AccII	CGCG	1 cut(s)	803
AciI	CCGC	4 cut(s)	5, 128, 138, 770
AclWI	GGATC	4 cut(s)	192, 221, 286, 308
AcsI	RAATTY	2 cut(s)	175, 555
AcuI	CTGAAG	2 cut(s)	68, 636
AfaI	GTAC	2 cut(s)	513, 668
AfiI	CCNNNNNNNGG	3 cut(s)	361, 890, 891
AgsI	TTSAA	9 cut(s)	181, 240, 259, 396, 403, 466, 590, 898, 928
AjnI	CCWGG	1 cut(s)	669
AluBI	AGCT	6 cut(s)	32, 78, 572, 650, 678, 862
AluI	AGCT	6 cut(s)	32, 78, 572, 650, 678, 862
Alw21I	GWGCWC	1 cut(s)	680
Alw26I	GTCTC	1 cut(s)	743
AlwI	GGATC	4 cut(s)	192, 221, 286, 308
Ama87I	CYCGRG	1 cut(s)	884
AoxI	GGCC	2 cut(s)	636, 761
ApeKI	GCWGC	5 cut(s)	8, 29, 344, 347, 572
ApoI	RAATTY	2 cut(s)	175, 555
Asp700I	GAANNNNTTC	2 cut(s)	660, 720
AspS9I	GGNCC	2 cut(s)	329, 372
AsuC2I	CCSGG	1 cut(s)	817
AsuHPI	GGTGA	6 cut(s)	127, 236, 421, 685, 868, 907
AvaI	CYCGRG	1 cut(s)	884
AvaII	GGWCC	2 cut(s)	329, 372
BaeGI	GKGCMC	1 cut(s)	700
BanI	GGYRCC	1 cut(s)	695
BanII	GRGCYC	1 cut(s)	680
Bbv12I	GWGCWC	1 cut(s)	680
BbvI	GCAGC	4 cut(s)	41, 331, 359, 559
BccI	CCATC	3 cut(s)	320, 503, 847
BceAI	ACGGC	2 cut(s)	261, 289
BciT130I	CCWGG	1 cut(s)	671
BciVI	GTATCC	1 cut(s)	103
BcnI	CCSGG	1 cut(s)	817
BcoDI	GTCTC	1 cut(s)	743
BfaI	CTAG	1 cut(s)	905
BfmI	CTRYAG	2 cut(s)	27, 345
BfuI	GTATCC	1 cut(s)	103
BisI	GCNGC	7 cut(s)	6, 9, 30, 129, 345, 348, 573
BlsI	GCNGC	7 cut(s)	7, 10, 31, 130, 346, 349, 574
Bme1390I	CCNGG	2 cut(s)	671, 817
Bme18I	GGWCC	2 cut(s)	329, 372
BmeT110I	CYCGRG	1 cut(s)	884
BmgT120I	GGNCC	2 cut(s)	329, 372
BmiI	GGNNCC	4 cut(s)	373, 528, 697, 826
BmrFI	CCNGG	2 cut(s)	671, 817
BmrI	ACTGGG	1 cut(s)	140
BmsI	GCATC	2 cut(s)	713, 792
BmuI	ACTGGG	1 cut(s)	140
BpuMI	CCSGG	1 cut(s)	817
BsaJI	CCNNGG	2 cut(s)	360, 885
Bsc4I	CCNNNNNNNGG	3 cut(s)	361, 890, 891
Bse1I	ACTGG	3 cut(s)	146, 332, 697
Bse3DI	GCAATG	1 cut(s)	639
BseBI	CCWGG	1 cut(s)	671
BseDI	CCNNGG	2 cut(s)	360, 885
BseLI	CCNNNNNNNGG	3 cut(s)	361, 890, 891
BseMI	GCAATG	1 cut(s)	639
BseNI	ACTGG	3 cut(s)	146, 332, 697
BseRI	GAGGAG	2 cut(s)	902, 929
BseSI	GKGCMC	1 cut(s)	700
BseXI	GCAGC	4 cut(s)	41, 331, 359, 559
BseYI	CCCAGC	1 cut(s)	32
Bsh1236I	CGCG	1 cut(s)	803
Bsh1285I	CGRYCG	1 cut(s)	684
BshFI	GGCC	2 cut(s)	638, 763
BshNI	GGYRCC	1 cut(s)	695
BsiEI	CGRYCG	1 cut(s)	684
BsiHKAI	GWGCWC	1 cut(s)	680
BsiHKCI	CYCGRG	1 cut(s)	884
BsiSI	CCGG	1 cut(s)	816
BslFI	GGGAC	1 cut(s)	385
BslI	CCNNNNNNNGG	3 cut(s)	361, 890, 891
BsmAI	GTCTC	1 cut(s)	743
BsmFI	GGGAC	1 cut(s)	385
BsnI	GGCC	2 cut(s)	638, 763
BsoBI	CYCGRG	1 cut(s)	884
Bsp1286I	GDGCHC	2 cut(s)	680, 700
Bsp1407I	TGTACA	1 cut(s)	511
Bsp143I	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
BspACI	CCGC	4 cut(s)	5, 128, 138, 770
BspANI	GGCC	2 cut(s)	638, 763
BspFNI	CGCG	1 cut(s)	803
BspLI	GGNNCC	4 cut(s)	373, 528, 697, 826
BspMAI	CTGCAG	2 cut(s)	31, 349
BspPI	GGATC	4 cut(s)	192, 221, 286, 308
BspT107I	GGYRCC	1 cut(s)	695
BsrDI	GCAATG	1 cut(s)	639
BsrGI	TGTACA	1 cut(s)	511
BsrI	ACTGG	3 cut(s)	146, 332, 697
BssECI	CCNNGG	2 cut(s)	360, 885
BssMI	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
BssT1I	CCWWGG	1 cut(s)	360
Bst2UI	CCWGG	1 cut(s)	671
Bst4CI	ACNGT	1 cut(s)	833
BstAUI	TGTACA	1 cut(s)	511
BstC8I	GCNNGC	1 cut(s)	302
BstDEI	CTNAG	1 cut(s)	74
BstFNI	CGCG	1 cut(s)	803
BstKTI	GATC	6 cut(s)	187, 229, 281, 316, 628, 684
BstMAI	GTCTC	1 cut(s)	743
BstMBI	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
BstMCI	CGRYCG	1 cut(s)	684
BstMWI	GCNNNNNNNGC	2 cut(s)	125, 137
BstNI	CCWGG	1 cut(s)	671
BstSCI	CCNGG	2 cut(s)	669, 815
BstSFI	CTRYAG	2 cut(s)	27, 345
BstSLI	GKGCMC	1 cut(s)	700
BstUI	CGCG	1 cut(s)	803
BstV1I	GCAGC	4 cut(s)	41, 331, 359, 559
BsuI	GTATCC	1 cut(s)	103
BsuRI	GGCC	2 cut(s)	638, 763
BtgZI	GCGATG	1 cut(s)	866
BtsI	GCAGTG	1 cut(s)	216
BtsIMutI	CAGTG	1 cut(s)	216
Cac8I	GCNNGC	1 cut(s)	302
Cfr13I	GGNCC	2 cut(s)	329, 372
CsiI	ACCWGGT	1 cut(s)	669
Csp6I	GTAC	2 cut(s)	512, 667
CviAII	CATG	2 cut(s)	487, 515
CviQI	GTAC	2 cut(s)	512, 667
DdeI	CTNAG	1 cut(s)	74
DpnI	GATC	6 cut(s)	186, 228, 280, 315, 627, 683
DpnII	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
DraI	TTTAAA	1 cut(s)	735
Ecl136II	GAGCTC	1 cut(s)	678
Eco130I	CCWWGG	1 cut(s)	360
Eco147I	AGGCCT	1 cut(s)	763
Eco24I	GRGCYC	1 cut(s)	680
Eco47I	GGWCC	2 cut(s)	329, 372
Eco53kI	GAGCTC	1 cut(s)	678
Eco57I	CTGAAG	2 cut(s)	68, 636
Eco88I	CYCGRG	1 cut(s)	884
EcoICRI	GAGCTC	1 cut(s)	678
EcoRII	CCWGG	1 cut(s)	669
EcoT14I	CCWWGG	1 cut(s)	360
EcoT38I	GRGCYC	1 cut(s)	680
ErhI	CCWWGG	1 cut(s)	360
FaeI	CATG	2 cut(s)	490, 518
FaiI	YATR	7 cut(s)	173, 287, 338, 448, 453, 488, 516
FaqI	GGGAC	1 cut(s)	385
FatI	CATG	2 cut(s)	486, 514
Fnu4HI	GCNGC	7 cut(s)	6, 9, 30, 129, 345, 348, 573
FriOI	GRGCYC	1 cut(s)	680
Fsp4HI	GCNGC	7 cut(s)	6, 9, 30, 129, 345, 348, 573
FspBI	CTAG	1 cut(s)	905
GluI	GCNGC	7 cut(s)	6, 9, 30, 129, 345, 348, 573
GsaI	CCCAGC	1 cut(s)	36
HaeIII	GGCC	2 cut(s)	638, 763
HapII	CCGG	1 cut(s)	816
Hin1II	CATG	2 cut(s)	490, 518
HindIII	AAGCTT	1 cut(s)	648
HinfI	GANTC	3 cut(s)	269, 412, 475
HpaII	CCGG	1 cut(s)	816
HphI	GGTGA	6 cut(s)	127, 236, 421, 685, 868, 907
Hpy166II	GTNNAC	3 cut(s)	444, 492, 512
Hpy188I	TCNGA	6 cut(s)	21, 283, 313, 655, 686, 944
Hpy188III	TCNNGA	4 cut(s)	230, 479, 530, 884
Hpy8I	GTNNAC	3 cut(s)	444, 492, 512
Hpy99I	CGWCG	1 cut(s)	833
HpyAV	CCTTC	7 cut(s)	175, 253, 397, 628, 650, 892, 922
HpyCH4III	ACNGT	1 cut(s)	833
HpyCH4V	TGCA	5 cut(s)	29, 200, 347, 386, 704
HpyF10VI	GCNNNNNNNGC	2 cut(s)	125, 137
HpyF3I	CTNAG	1 cut(s)	74
Hsp92II	CATG	2 cut(s)	490, 518
Kzo9I	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
LmnI	GCTCC	2 cut(s)	124, 824
Lsp1109I	GCAGC	4 cut(s)	41, 331, 359, 559
LweI	GCATC	2 cut(s)	713, 792
MabI	ACCWGGT	1 cut(s)	669
MaeI	CTAG	1 cut(s)	905
MaeIII	GTNAC	1 cut(s)	688
MalI	GATC	6 cut(s)	186, 228, 280, 315, 627, 683
MboI	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
MboII	GAAGA	2 cut(s)	6, 61
MfeI	CAATTG	3 cut(s)	419, 432, 621
MhlI	GDGCHC	2 cut(s)	680, 700
MluCI	AATT	9 cut(s)	175, 293, 387, 419, 432, 555, 590, 621, 741
MmeI	TCCRAC	1 cut(s)	67
MnlI	CCTC	6 cut(s)	167, 362, 400, 538, 880, 883
MroXI	GAANNNNTTC	2 cut(s)	660, 720
MseI	TTAA	3 cut(s)	560, 734, 744
MspA1I	CMGCKG	1 cut(s)	32
MspI	CCGG	1 cut(s)	816
MspR9I	CCNGG	2 cut(s)	671, 817
MunI	CAATTG	3 cut(s)	419, 432, 621
MvaI	CCWGG	1 cut(s)	671
MvnI	CGCG	1 cut(s)	803
MwoI	GCNNNNNNNGC	2 cut(s)	125, 137
NciI	CCSGG	1 cut(s)	817
NdeII	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
NlaIII	CATG	2 cut(s)	490, 518
NlaIV	GGNNCC	4 cut(s)	373, 528, 697, 826
PceI	AGGCCT	1 cut(s)	763
PcsI	WCGNNNNNNNCGW	2 cut(s)	297, 837
PdmI	GAANNNNTTC	2 cut(s)	660, 720
PfeI	GAWTC	3 cut(s)	269, 412, 475
PkrI	GCNGC	7 cut(s)	7, 10, 31, 130, 346, 349, 574
Ple19I	CGATCG	1 cut(s)	684
Psp124BI	GAGCTC	1 cut(s)	680
Psp6I	CCWGG	1 cut(s)	669
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	1 cut(s)	669
PspN4I	GGNNCC	4 cut(s)	373, 528, 697, 826
PspPI	GGNCC	2 cut(s)	329, 372
PstI	CTGCAG	2 cut(s)	31, 349
PvuI	CGATCG	1 cut(s)	684
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	2 cut(s)	513, 668
RsaNI	GTAC	2 cut(s)	512, 667
SacI	GAGCTC	1 cut(s)	680
SaqAI	TTAA	3 cut(s)	560, 734, 744
SatI	GCNGC	7 cut(s)	6, 9, 30, 129, 345, 348, 573
Sau3AI	GATC	6 cut(s)	184, 226, 278, 313, 625, 681
Sau96I	GGNCC	2 cut(s)	329, 372
ScrFI	CCNGG	2 cut(s)	671, 817
SduI	GDGCHC	2 cut(s)	680, 700
SexAI	ACCWGGT	1 cut(s)	669
SfaNI	GCATC	2 cut(s)	713, 792
SfcI	CTRYAG	2 cut(s)	27, 345
SinI	GGWCC	2 cut(s)	329, 372
Sse9I	AATT	9 cut(s)	175, 293, 387, 419, 432, 555, 590, 621, 741
SseBI	AGGCCT	1 cut(s)	763
SsiI	CCGC	4 cut(s)	5, 128, 138, 770
SspI	AATATT	1 cut(s)	586
SspMI	CTAG	1 cut(s)	905
SstI	GAGCTC	1 cut(s)	680
StuI	AGGCCT	1 cut(s)	763
StyD4I	CCNGG	2 cut(s)	669, 815
StyI	CCWWGG	1 cut(s)	360
TaaI	ACNGT	1 cut(s)	833
TaqI	TCGA	4 cut(s)	104, 291, 628, 680
TasI	AATT	9 cut(s)	175, 293, 387, 419, 432, 555, 590, 621, 741
TatI	WGTACW	1 cut(s)	511
TauI	GCSGC	2 cut(s)	8, 131
TfiI	GAWTC	3 cut(s)	269, 412, 475
Tru1I	TTAA	3 cut(s)	560, 734, 744
Tru9I	TTAA	3 cut(s)	560, 734, 744
TscAI	CASTG	1 cut(s)	223
TseI	GCWGC	5 cut(s)	8, 29, 344, 347, 572
TspDTI	ATGAA	4 cut(s)	160, 475, 647, 729
TspRI	CASTG	1 cut(s)	223
VpaK11BI	GGWCC	2 cut(s)	329, 372
XapI	RAATTY	2 cut(s)	175, 555
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XmnI	GAANNNNTTC	2 cut(s)	660, 720
XspI	CTAG	1 cut(s)	905

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.