MD05G1262500.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002150
Physical Location & Seq
Reverse (-)
39741299 .. 39742859
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262500.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGACACCCTTTACCTTGTTCTCTCTTTGGGAGCTGCAGTTTTCGCTTTGACCGTCTTTGCATTCAAGCGAAATTCAGATGACAGTAACAACCTACCGCCAGGGAGCATGGGGTGGCCTATTGTGGGAGAAACTCTCGAGTTTCTGTTTGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGGAGGTACTCTCCTGACATCTTCAAGACCAAAATTCTTGGAGAGAAAACTGCTGTCATTTGTGGTCCTAATGGACACAAGTTTCTGTTCTCCAATGAACAAAAGTACTTCACAGCTTTTCGACCACATTCCATGCAGAAGATGTTTCGTTCGTACAAGGCTGCTGCTGCAGCTTCTGCTCCTCCTGCAGCACAGCCTGCTCGTAATGAAGAAGCGAAAGTATTGAGGTCCCCAGGGTTTTTGAAGCCGGAAGCATTGGTGAGGTACTTGGGAAAAATGGACTCAATAACCCAAGAACAGATGAGAACCTGTTGGGAAGGAAAAGATGAGGTCAAGGTCTACCCTTTGGCCAAGAAACTCACTCTAAGTCTTGCCTGCAGATTCTTCTTGGGCATCGATGATCCCGAGAAAATTGCAAGCCTAGTGAGCCACTTTGATGATGTTACTGTTGGGATGCATTCACTTATTGTGAACTTCCCAGGAACAATATTCTTTAAGGCAACCAAAGCTGCAGATGCGCTGCGAAAGGAGTTGAAGACTGTGATTCAGGAGAAGAAGGCTGCAATGGCATCGGGAGCGCCTATGCATGACATTTTGTCACATATGATTATGACCAGTGATCCATCTGGAAAATACATGCCGGAGGCAGAGGTTGCCGATAAGATAATGGGTTTACTCACAGCAGGATATAGCACTGTGTCTACTGCCAAGACTTTCTTCATGAAGTATGTGGGAGAGAAGCCAGACATTTATGCCAAGGTCCTAGCTGAGCACAAAGAGATTGCAGACTCAAAGAAGCCAGAAGACTTACTGGAGTGGGAAGACATCAACAAGATGAAGTACTCATGGAATGTGTTGCATGAAGTGATGAGATTCACACCGCCACTTCAAGGAACATTCAGAGAGGCCTTGACTGATTTTACCTACGCCGGTTACACCATTCTGAAGGGCTGGAAGGTATACTGGACTGTCAGTACAACAAACATGAACCCGGAATACTTCCCCAACCCGGAAAAGTTTGATCCATCAAGATATGATGACCTAAGTGCATTCCCAGCTTTTACATTCGTTCCATTTGGAGGAGGGCCAAGAATGTGCCCCGGGAAAGAGTACGCTCGCCTAGCAATCCTCACCTTCGTTCACAATGTGGTGAAGAGGTTCGAATGGGAAGTGCTATTTCCTAAGGAGAAGATCACAGGCGACATGATGCCAACACCGGAGAAAGGGCTCCCAGTTCGCCTTCATTGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

479

Amino Acids

54.05

Weight (kDa)

8.93

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 453 4.1e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 522, 884, 1143
AciI CCGC 2 cut(s) 98, 1064
AclWI GGATC 3 cut(s) 578, 797, 1199
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 3 cut(s) 73, 163, 216
AcuI CTGAAG 1 cut(s) 1148
AdeI CACNNNGTG 1 cut(s) 1330
AfaI GTAC 7 cut(s) 191, 290, 338, 449, 1025, 1159, 1295
AfiI CCNNNNNNNGG 5 cut(s) 125, 1073, 1192, 1262, 1406
AgsI TTSAA 6 cut(s) 67, 175, 208, 427, 718, 1073
AhdI GACNNNNNGTC 1 cut(s) 778
AjnI CCWGG 3 cut(s) 100, 415, 661
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
AluBI AGCT 6 cut(s) 35, 299, 356, 692, 950, 1241
AluI AGCT 6 cut(s) 35, 299, 356, 692, 950, 1241
Alw21I GWGCWC 1 cut(s) 957
AlwI GGATC 3 cut(s) 578, 797, 1199
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 3 cut(s) 137, 587, 1283
AoxI GGCC 4 cut(s) 116, 531, 1089, 1268
ApeKI GCWGC 9 cut(s) 35, 344, 347, 350, 353, 371, 692, 703, 743
ApoI RAATTY 3 cut(s) 73, 163, 216
Asp700I GAANNNNTTC 1 cut(s) 1340
AspLEI GCGC 2 cut(s) 703, 763
AspS9I GGNCC 4 cut(s) 248, 411, 943, 1268
AsuC2I CCSGG 4 cut(s) 1175, 1193, 1284, 1285
AsuHPI GGTGA 3 cut(s) 454, 1306, 1345
AsuII TTCGAA 1 cut(s) 1344
AvaI CYCGRG 3 cut(s) 137, 587, 1283
AvaII GGWCC 3 cut(s) 248, 411, 943
AxyI CCTNAGG 1 cut(s) 1365
BaeGI GKGCMC 1 cut(s) 1283
BalI TGGCCA 1 cut(s) 533
BanII GRGCYC 1 cut(s) 1413
BbsI GAAGAC 3 cut(s) 725, 993, 1011
Bbv12I GWGCWC 1 cut(s) 957
BbvI GCAGC 9 cut(s) 22, 331, 334, 337, 365, 383, 679, 690, 730
BccI CCATC 2 cut(s) 814, 1216
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 735, 769
BciT130I CCWGG 3 cut(s) 102, 417, 663
BcnI CCSGG 4 cut(s) 1175, 1193, 1284, 1285
BfaI CTAG 4 cut(s) 605, 947, 1304, 1435
BfmI CTRYAG 5 cut(s) 36, 351, 369, 559, 693
BfoI RGCGCY 1 cut(s) 764
BisI GCNGC 9 cut(s) 36, 345, 348, 351, 354, 372, 693, 704, 744
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 9 cut(s) 37, 346, 349, 352, 355, 373, 694, 705, 745
BmcAI AGTACT 2 cut(s) 290, 1025
Bme1390I CCNGG 7 cut(s) 102, 417, 663, 1175, 1193, 1284, 1285
Bme18I GGWCC 3 cut(s) 248, 411, 943
BmeRI GACNNNNNGTC 1 cut(s) 778
BmeT110I CYCGRG 3 cut(s) 137, 587, 1283
BmgT120I GGNCC 4 cut(s) 248, 411, 943, 1268
BmiI GGNNCC 2 cut(s) 413, 1412
BmrFI CCNGG 7 cut(s) 102, 417, 663, 1175, 1193, 1284, 1285
BmrI ACTGGG 1 cut(s) 1409
BmsI GCATC 5 cut(s) 585, 627, 688, 761, 1380
BmuI ACTGGG 1 cut(s) 1409
BpiI GAAGAC 3 cut(s) 725, 993, 1011
BplI GAGNNNNNCTC 4 cut(s) 120, 152, 178, 210
BpmI CTGGAG 1 cut(s) 1016
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 1344
BpuMI CCSGG 4 cut(s) 1175, 1193, 1284, 1285
Bsa29I ATCGAT 1 cut(s) 579
BsaJI CCNNGG 7 cut(s) 101, 415, 416, 661, 939, 1282, 1283
BsaWI WCCGGW 1 cut(s) 1399
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 1073, 1192, 1262, 1406
Bse118I RCCGGY 1 cut(s) 1112
Bse1I ACTGG 4 cut(s) 798, 999, 1151, 1415
Bse21I CCTNAGG 1 cut(s) 1365
Bse3DI GCAATG 2 cut(s) 753, 1426
BseBI CCWGG 3 cut(s) 102, 417, 663
BseCI ATCGAT 1 cut(s) 579
BseDI CCNNGG 7 cut(s) 101, 415, 416, 661, 939, 1282, 1283
BseGI GGATG 2 cut(s) 186, 642
BseLI CCNNNNNNNGG 5 cut(s) 125, 1073, 1192, 1262, 1406
BseMI GCAATG 2 cut(s) 753, 1426
BseMII CTCAG 1 cut(s) 942
BseNI ACTGG 4 cut(s) 798, 999, 1151, 1415
BseRI GAGGAG 3 cut(s) 199, 354, 1278
BseSI GKGCMC 1 cut(s) 1283
BseXI GCAGC 9 cut(s) 22, 331, 334, 337, 365, 383, 679, 690, 730
BseYI CCCAGC 1 cut(s) 1237
BshFI GGCC 4 cut(s) 118, 533, 1091, 1270
BshVI ATCGAT 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 3 cut(s) 137, 587, 1283
BsiSI CCGG 8 cut(s) 158, 431, 824, 1113, 1175, 1193, 1284, 1400
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 5 cut(s) 125, 1073, 1192, 1262, 1406
BsmFI GGGAC 1 cut(s) 397
BsmI GAATGC 3 cut(s) 62, 640, 1232
BsnI GGCC 4 cut(s) 118, 533, 1091, 1270
BsoBI CYCGRG 3 cut(s) 137, 587, 1283
Bsp119I TTCGAA 1 cut(s) 1344
Bsp1286I GDGCHC 3 cut(s) 957, 1283, 1413
Bsp143I GATC 4 cut(s) 583, 802, 1204, 1374
Bsp1720I GCTNAGC 1 cut(s) 951
BspACI CCGC 2 cut(s) 98, 1064
BspANI GGCC 4 cut(s) 118, 533, 1091, 1270
BspCNI CTCAG 1 cut(s) 943
BspDI ATCGAT 1 cut(s) 579
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 2 cut(s) 413, 1412
BspMAI CTGCAG 5 cut(s) 40, 355, 373, 563, 697
BspPI GGATC 3 cut(s) 578, 797, 1199
BspT104I TTCGAA 1 cut(s) 1344
BsrDI GCAATG 2 cut(s) 753, 1426
BsrFI RCCGGY 1 cut(s) 1112
BsrI ACTGG 4 cut(s) 798, 999, 1151, 1415
BssAI RCCGGY 1 cut(s) 1112
BssECI CCNNGG 7 cut(s) 101, 415, 416, 661, 939, 1282, 1283
BssMI GATC 4 cut(s) 583, 802, 1204, 1374
BssNAI GTATAC 1 cut(s) 1144
BssT1I CCWWGG 1 cut(s) 939
Bst1107I GTATAC 1 cut(s) 1144
Bst2UI CCWGG 3 cut(s) 102, 417, 663
Bst4CI ACNGT 6 cut(s) 55, 86, 631, 724, 880, 1153
Bst6I CTCTTC 1 cut(s) 1331
BstAPI GCANNNNNTGC 3 cut(s) 359, 380, 836
BstBI TTCGAA 1 cut(s) 1344
BstC8I GCNNGC 4 cut(s) 381, 559, 601, 1300
BstDEI CTNAG 4 cut(s) 548, 951, 1226, 1365
BstF5I GGATG 2 cut(s) 186, 642
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 2 cut(s) 703, 763
BstKTI GATC 4 cut(s) 586, 805, 1207, 1377
BstMBI GATC 4 cut(s) 583, 802, 1204, 1374
BstNI CCWGG 3 cut(s) 102, 417, 663
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 7 cut(s) 100, 415, 661, 1173, 1191, 1282, 1283
BstSFI CTRYAG 5 cut(s) 36, 351, 369, 559, 693
BstSLI GKGCMC 1 cut(s) 1283
BstV1I GCAGC 9 cut(s) 22, 331, 334, 337, 365, 383, 679, 690, 730
BstV2I GAAGAC 3 cut(s) 725, 993, 1011
BstZ17I GTATAC 1 cut(s) 1144
Bsu15I ATCGAT 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 1365
BsuRI GGCC 4 cut(s) 118, 533, 1091, 1270
BsuTUI ATCGAT 1 cut(s) 579
BtsCI GGATG 2 cut(s) 186, 642
BtsIMutI CAGTG 2 cut(s) 805, 876
Cac8I GCNNGC 4 cut(s) 381, 559, 601, 1300
CaiI CAGNNNCTG 1 cut(s) 359
CciI TCATGA 1 cut(s) 903
CfoI GCGC 2 cut(s) 703, 763
Cfr10I RCCGGY 1 cut(s) 1112
Cfr13I GGNCC 4 cut(s) 248, 411, 943, 1268
Cfr9I CCCGGG 1 cut(s) 1283
ClaI ATCGAT 1 cut(s) 579
Csp6I GTAC 7 cut(s) 190, 289, 337, 448, 1024, 1158, 1294
CviAII CATG 9 cut(s) 109, 316, 770, 820, 904, 1029, 1043, 1168, 1387
CviQI GTAC 7 cut(s) 190, 289, 337, 448, 1024, 1158, 1294
DdeI CTNAG 4 cut(s) 548, 951, 1226, 1365
DpnI GATC 4 cut(s) 585, 804, 1206, 1376
DpnII GATC 4 cut(s) 583, 802, 1204, 1374
DraIII CACNNNGTG 1 cut(s) 1330
DriI GACNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 1331
Eam1105I GACNNNNNGTC 1 cut(s) 778
EarI CTCTTC 1 cut(s) 1331
Eco130I CCWWGG 1 cut(s) 939
Eco147I AGGCCT 1 cut(s) 1091
Eco24I GRGCYC 1 cut(s) 1413
Eco47I GGWCC 3 cut(s) 248, 411, 943
Eco57I CTGAAG 1 cut(s) 1148
Eco81I CCTNAGG 1 cut(s) 1365
Eco88I CYCGRG 3 cut(s) 137, 587, 1283
EcoO109I RGGNCCY 2 cut(s) 411, 943
EcoRII CCWGG 3 cut(s) 100, 415, 661
EcoT14I CCWWGG 1 cut(s) 939
EcoT22I ATGCAT 2 cut(s) 642, 771
EcoT38I GRGCYC 1 cut(s) 1413
ErhI CCWWGG 1 cut(s) 939
FaeI CATG 9 cut(s) 112, 319, 773, 823, 907, 1032, 1046, 1171, 1390
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 1 cut(s) 397
FatI CATG 9 cut(s) 108, 315, 769, 819, 903, 1028, 1042, 1167, 1386
FauNDI CATATG 1 cut(s) 786
FblI GTMKAC 3 cut(s) 522, 884, 1143
Fnu4HI GCNGC 9 cut(s) 36, 345, 348, 351, 354, 372, 693, 704, 744
FokI GGATG 2 cut(s) 193, 649
FriOI GRGCYC 1 cut(s) 1413
Fsp4HI GCNGC 9 cut(s) 36, 345, 348, 351, 354, 372, 693, 704, 744
FspBI CTAG 4 cut(s) 605, 947, 1304, 1435
GlaI GCGC 2 cut(s) 702, 762
GluI GCNGC 9 cut(s) 36, 345, 348, 351, 354, 372, 693, 704, 744
GsaI CCCAGC 1 cut(s) 1241
GsuI CTGGAG 1 cut(s) 1016
HaeII RGCGCY 1 cut(s) 764
HaeIII GGCC 4 cut(s) 118, 533, 1091, 1270
HapII CCGG 8 cut(s) 158, 431, 824, 1113, 1175, 1193, 1284, 1400
HhaI GCGC 2 cut(s) 703, 763
Hin1II CATG 9 cut(s) 112, 319, 773, 823, 907, 1032, 1046, 1171, 1390
Hin6I GCGC 2 cut(s) 701, 761
HinP1I GCGC 2 cut(s) 701, 761
HinfI GANTC 5 cut(s) 464, 564, 727, 971, 1056
HpaII CCGG 8 cut(s) 158, 431, 824, 1113, 1175, 1193, 1284, 1400
HphI GGTGA 3 cut(s) 454, 1306, 1345
Hpy166II GTNNAC 6 cut(s) 523, 655, 857, 885, 1144, 1324
Hpy188I TCNGA 3 cut(s) 79, 1085, 1128
Hpy8I GTNNAC 6 cut(s) 523, 655, 857, 885, 1144, 1324
HpyAV CCTTC 6 cut(s) 494, 733, 1123, 1132, 1327, 1433
HpyCH4III ACNGT 6 cut(s) 55, 86, 631, 724, 880, 1153
HpyF3I CTNAG 4 cut(s) 548, 951, 1226, 1365
Hsp92II CATG 9 cut(s) 112, 319, 773, 823, 907, 1032, 1046, 1171, 1390
HspAI GCGC 2 cut(s) 701, 761
Kzo9I GATC 4 cut(s) 583, 802, 1204, 1374
LmnI GCTCC 5 cut(s) 32, 105, 367, 758, 1416
Lsp1109I GCAGC 9 cut(s) 22, 331, 334, 337, 365, 383, 679, 690, 730
LweI GCATC 5 cut(s) 585, 627, 688, 761, 1380
MaeI CTAG 4 cut(s) 605, 947, 1304, 1435
MaeIII GTNAC 4 cut(s) 86, 625, 780, 1115
MalI GATC 4 cut(s) 585, 804, 1206, 1376
MboI GATC 4 cut(s) 583, 802, 1204, 1374
MhlI GDGCHC 3 cut(s) 957, 1283, 1413
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 4 cut(s) 73, 163, 216, 594
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 2 cut(s) 458, 965
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 2 cut(s) 642, 771
MroXI GAANNNNTTC 1 cut(s) 1340
MscI TGGCCA 1 cut(s) 533
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 533
MspI CCGG 8 cut(s) 158, 431, 824, 1113, 1175, 1193, 1284, 1400
MspR9I CCNGG 7 cut(s) 102, 417, 663, 1175, 1193, 1284, 1285
Mva1269I GAATGC 3 cut(s) 62, 640, 1232
MvaI CCWGG 3 cut(s) 102, 417, 663
NciI CCSGG 4 cut(s) 1175, 1193, 1284, 1285
NdeI CATATG 1 cut(s) 786
NdeII GATC 4 cut(s) 583, 802, 1204, 1374
NlaIII CATG 9 cut(s) 112, 319, 773, 823, 907, 1032, 1046, 1171, 1390
NlaIV GGNNCC 2 cut(s) 413, 1412
NmuCI GTSAC 1 cut(s) 780
NsiI ATGCAT 2 cut(s) 642, 771
NspI RCATGY 1 cut(s) 823
NspV TTCGAA 1 cut(s) 1344
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 903
PasI CCCWGGG 1 cut(s) 416
PceI AGGCCT 1 cut(s) 1091
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 585
PctI GAATGC 3 cut(s) 62, 640, 1232
PdmI GAANNNNTTC 1 cut(s) 1340
PfeI GAWTC 3 cut(s) 564, 727, 1056
PkrI GCNGC 9 cut(s) 37, 346, 349, 352, 355, 373, 694, 705, 745
PleI GAGTC 2 cut(s) 458, 965
PpsI GAGTC 2 cut(s) 458, 965
PpuMI RGGWCCY 2 cut(s) 411, 943
Psp5II RGGWCCY 2 cut(s) 411, 943
Psp6I CCWGG 3 cut(s) 100, 415, 661
PspFI CCCAGC 1 cut(s) 1237
PspGI CCWGG 3 cut(s) 100, 415, 661
PspN4I GGNNCC 2 cut(s) 413, 1412
PspPI GGNCC 4 cut(s) 248, 411, 943, 1268
PspPPI RGGWCCY 2 cut(s) 411, 943
PsrI GAACNNNNNNTAC 2 cut(s) 1163, 1195
PstI CTGCAG 5 cut(s) 40, 355, 373, 563, 697
PstNI CAGNNNCTG 1 cut(s) 359
RsaI GTAC 7 cut(s) 191, 290, 338, 449, 1025, 1159, 1295
RsaNI GTAC 7 cut(s) 190, 289, 337, 448, 1024, 1158, 1294
RseI CAYNNNNRTG 1 cut(s) 618
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 9 cut(s) 36, 345, 348, 351, 354, 372, 693, 704, 744
Sau3AI GATC 4 cut(s) 583, 802, 1204, 1374
Sau96I GGNCC 4 cut(s) 248, 411, 943, 1268
ScaI AGTACT 2 cut(s) 290, 1025
SchI GAGTC 2 cut(s) 458, 965
ScrFI CCNGG 7 cut(s) 102, 417, 663, 1175, 1193, 1284, 1285
SduI GDGCHC 3 cut(s) 957, 1283, 1413
SfaNI GCATC 5 cut(s) 585, 627, 688, 761, 1380
SfcI CTRYAG 5 cut(s) 36, 351, 369, 559, 693
Sfr274I CTCGAG 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1344
SinI GGWCC 3 cut(s) 248, 411, 943
SlaI CTCGAG 1 cut(s) 137
SmaI CCCGGG 1 cut(s) 1285
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 4 cut(s) 73, 163, 216, 594
SseBI AGGCCT 1 cut(s) 1091
SsiI CCGC 2 cut(s) 98, 1064
SspI AATATT 1 cut(s) 672
SspMI CTAG 4 cut(s) 605, 947, 1304, 1435
StuI AGGCCT 1 cut(s) 1091
StyD4I CCNGG 7 cut(s) 100, 415, 661, 1173, 1191, 1282, 1283
StyI CCWWGG 1 cut(s) 939
TaaI ACNGT 6 cut(s) 55, 86, 631, 724, 880, 1153
TaqI TCGA 4 cut(s) 138, 304, 579, 1344
TasI AATT 4 cut(s) 73, 163, 216, 594
TatI WGTACW 3 cut(s) 288, 1023, 1157
TfiI GAWTC 3 cut(s) 564, 727, 1056
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 2 cut(s) 805, 883
TseFI GTSAC 1 cut(s) 780
TseI GCWGC 9 cut(s) 35, 344, 347, 350, 353, 371, 692, 703, 743
Tsp45I GTSAC 1 cut(s) 780
TspDTI ATGAA 8 cut(s) 294, 405, 892, 920, 1034, 1059, 1184, 1415
TspMI CCCGGG 1 cut(s) 1283
TspRI CASTG 2 cut(s) 805, 883
VpaK11BI GGWCC 3 cut(s) 248, 411, 943
XapI RAATTY 3 cut(s) 73, 163, 216
XceI RCATGY 1 cut(s) 823
XhoI CTCGAG 1 cut(s) 137
XmaI CCCGGG 1 cut(s) 1283
XmiI GTMKAC 3 cut(s) 522, 884, 1143
XmnI GAANNNNTTC 1 cut(s) 1340
XspI CTAG 4 cut(s) 605, 947, 1304, 1435
ZrmI AGTACT 2 cut(s) 290, 1025
Zsp2I ATGCAT 2 cut(s) 642, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.