MD05G1262700.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002150
Physical Location & Seq
Reverse (-)
39755570 .. 39757278
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262700.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGGAAACCCTTTACCTTGCTTTATCTTTGGGAGCTGCTTTTTTAGCCTTTATCATATTTGCTTTCAAAGGCAAATCAGATGATGGCAAAAACCTTCCACCAGGGAGCATGGGGTGGCCTATTGTGGGTGAATCAATCGAGTTCCTGTTCGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGGAGGTACTCTCCTGACATCTTCAAGACCTATATTCTTGGGGAAAAAACTGCAGTGATTTGTGGTCCTAGTGGACACAAATTTCTGTTCTCCAATGAACAAAAGTACTTCACAGCATTCCGACCACATTCGATGCAAAAGATGTTTCGATCATACAAGGCTGCCGCGCCCACTGCTTCTGCTGCTCCCGCTGTGGCACAACCTTCTCGCGATGAAGAAGCGAAAGTTATAAGATCCCCAGGGTTTTTGAAGCCGGAAGCATTGGTGAGGTACTTGGGGAAAATGGACTCTATCACTCAGGAACAGATGAAGGCCTATTGGGAAGGCAAAGATGAGGTCGAGGTCTACCCCTTGGCCAAGACCCTCACTCTAAGTCTTGCCTGCAGATTCTTCTTGGGTATCGATGATTCCGAGCGAATTGCGAGGCTTGTGAGCAATTTTGATGATGTTACCGTTGGGATGCATTCGCTTATTATCAACTTCCCAGGAACAACATTCTACAAGGCAACCAAAGCCGCAGACGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCGATGGCGGCAGGAGGTCCCATGCATGATATATTGTCACACATGATTGTGGCTAGTGACCCATCTGGCAAACACATGCCTGAGGCTGAGGTTGCGGATAAGATCATGGGTTTGCTCACAGCAGGATATAGCACTGTGGCTACTGCCATGACTTTCTTCATGAAATACGTTGGAGAGAGGCCAGACATCTATGCCAAGGTTCTAGCTGAGCACAAGGAGATAGCAGACTCAAAGAAGCCAGGAGACTTCTTGGAATGGGAAGACATCAACAAAATGAAATATTCATGGAATGTGTTGTATGAAGTGATGAGATTCACGCCGCCACTTCAAGGAACATTCAGAGAGGCCTTGACTGATTTTACCTACGCCGGTTACACCATTCCGAAGGGCTGGAAGGTATATTGGACTGTGAGTACAGTAAACATGAACTCAGAGTACTTCCCCAACCCAGAAAAGTTTGACCCATCAAGATATGATGACCTAAGTGTATTCCCAGCTTTCACATTTGTTCCATTTGGAGGAGGCCCAAGAATGTGCCCTGGGAAAGAGTACGCTCGCCTAGCAATCCTCACTTTCGTTCACAATGTGGTCAAGAGGTTCAAATGGGAAGTGGTATTTCCTAAGGAGAAGATCACCGGCGACATGATGCCAACACCGGAAAAAGGACTTCCGGTTCGCCTTACCCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

481

Amino Acids

54.21

Weight (kDa)

9.0

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 455 2.2e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AccI GTMKAC 1 cut(s) 528
AccII CGCG 3 cut(s) 350, 393, 707
AciI CCGC 6 cut(s) 348, 372, 699, 758, 845, 1070
AclWI GGATC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 163, 263
AdeI CACNNNGTG 1 cut(s) 1336
AfaI GTAC 6 cut(s) 191, 290, 455, 1165, 1187, 1301
AfiI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
AgsI TTSAA 6 cut(s) 67, 175, 208, 433, 1079, 1351
AjnI CCWGG 5 cut(s) 100, 421, 667, 988, 1288
AloI GAACNNNNNNTCC 2 cut(s) 1407, 1439
AluBI AGCT 3 cut(s) 35, 956, 1247
AluI AGCT 3 cut(s) 35, 956, 1247
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 987
AlwI GGATC 1 cut(s) 411
AoxI GGCC 6 cut(s) 116, 495, 537, 929, 1095, 1273
ApeKI GCWGC 5 cut(s) 35, 344, 365, 709, 749
ApoI RAATTY 2 cut(s) 163, 263
ArsI GACNNNNNNTTYG 4 cut(s) 504, 536, 1407, 1439
AspLEI GCGC 2 cut(s) 352, 709
AspS9I GGNCC 3 cut(s) 248, 767, 1274
AsuHPI GGTGA 3 cut(s) 140, 460, 1375
AvaII GGWCC 2 cut(s) 248, 767
AxyI CCTNAGG 2 cut(s) 831, 1371
BaeGI GKGCMC 1 cut(s) 1289
BalI TGGCCA 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 5 cut(s) 22, 331, 352, 696, 736
BccI CCATC 4 cut(s) 77, 748, 820, 1222
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 5 cut(s) 102, 423, 669, 990, 1290
BcoDI GTCTC 1 cut(s) 987
BfaI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
BfmI CTRYAG 2 cut(s) 234, 565
BisI GCNGC 9 cut(s) 36, 345, 348, 366, 699, 710, 750, 759, 1070
BlpI GCTNAGC 1 cut(s) 957
BlsI GCNGC 9 cut(s) 37, 346, 349, 367, 700, 711, 751, 760, 1071
BmcAI AGTACT 2 cut(s) 290, 1187
Bme1390I CCNGG 5 cut(s) 102, 423, 669, 990, 1290
Bme18I GGWCC 2 cut(s) 248, 767
BmgT120I GGNCC 3 cut(s) 248, 767, 1274
BmiI GGNNCC 1 cut(s) 769
BmrFI CCNGG 5 cut(s) 102, 423, 669, 990, 1290
BmsI GCATC 3 cut(s) 306, 633, 1386
BpiI GAAGAC 1 cut(s) 1017
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 1 cut(s) 837
Bpu1102I GCTNAGC 1 cut(s) 957
Bsa29I ATCGAT 1 cut(s) 585
BsaJI CCNNGG 8 cut(s) 101, 421, 422, 534, 667, 945, 1288, 1289
BsaWI WCCGGW 2 cut(s) 1405, 1420
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
Bse118I RCCGGY 2 cut(s) 1118, 1385
Bse21I CCTNAGG 2 cut(s) 831, 1371
BseBI CCWGG 5 cut(s) 102, 423, 669, 990, 1290
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 8 cut(s) 101, 421, 422, 534, 667, 945, 1288, 1289
BseGI GGATG 2 cut(s) 186, 648
BseLI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
BseMII CTCAG 5 cut(s) 494, 822, 828, 948, 1194
BseRI GAGGAG 2 cut(s) 199, 1284
BseSI GKGCMC 1 cut(s) 1289
BseXI GCAGC 5 cut(s) 22, 331, 352, 696, 736
BseYI CCCAGC 1 cut(s) 1243
Bsh1236I CGCG 3 cut(s) 350, 393, 707
BshFI GGCC 6 cut(s) 118, 497, 539, 931, 1097, 1275
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 963
BsiSI CCGG 6 cut(s) 158, 437, 1119, 1386, 1406, 1421
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 4 cut(s) 125, 1079, 1268, 1412
BsmAI GTCTC 1 cut(s) 987
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 2 cut(s) 299, 646
BsnI GGCC 6 cut(s) 118, 497, 539, 931, 1097, 1275
Bsp1286I GDGCHC 2 cut(s) 963, 1289
Bsp143I GATC 4 cut(s) 332, 416, 852, 1380
Bsp1720I GCTNAGC 1 cut(s) 957
Bsp68I TCGCGA 1 cut(s) 393
BspACI CCGC 6 cut(s) 348, 372, 699, 758, 845, 1070
BspANI GGCC 6 cut(s) 118, 497, 539, 931, 1097, 1275
BspCNI CTCAG 5 cut(s) 493, 823, 829, 949, 1193
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 3 cut(s) 350, 393, 707
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 1 cut(s) 769
BspMAI CTGCAG 2 cut(s) 238, 569
BspPI GGATC 1 cut(s) 411
BsrFI RCCGGY 2 cut(s) 1118, 1385
BssAI RCCGGY 2 cut(s) 1118, 1385
BssECI CCNNGG 8 cut(s) 101, 421, 422, 534, 667, 945, 1288, 1289
BssMI GATC 4 cut(s) 332, 416, 852, 1380
BssT1I CCWWGG 2 cut(s) 534, 945
Bst2UI CCWGG 5 cut(s) 102, 423, 669, 990, 1290
Bst4CI ACNGT 4 cut(s) 637, 886, 1159, 1168
BstC8I GCNNGC 2 cut(s) 565, 1306
BstDEI CTNAG 8 cut(s) 480, 554, 831, 837, 957, 1180, 1232, 1371
BstF5I GGATG 2 cut(s) 186, 648
BstFNI CGCG 3 cut(s) 350, 393, 707
BstHHI GCGC 2 cut(s) 352, 709
BstKTI GATC 4 cut(s) 335, 419, 855, 1383
BstMAI GTCTC 1 cut(s) 987
BstMBI GATC 4 cut(s) 332, 416, 852, 1380
BstNI CCWGG 5 cut(s) 102, 423, 669, 990, 1290
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 5 cut(s) 100, 421, 667, 988, 1288
BstSFI CTRYAG 2 cut(s) 234, 565
BstSLI GKGCMC 1 cut(s) 1289
BstUI CGCG 3 cut(s) 350, 393, 707
BstV1I GCAGC 5 cut(s) 22, 331, 352, 696, 736
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 585
Bsu36I CCTNAGG 2 cut(s) 831, 1371
BsuRI GGCC 6 cut(s) 118, 497, 539, 931, 1097, 1275
BsuTUI ATCGAT 1 cut(s) 585
BtgZI GCGATG 2 cut(s) 408, 767
BtsCI GGATG 2 cut(s) 186, 648
BtsI GCAGTG 2 cut(s) 243, 354
BtsIMutI CAGTG 3 cut(s) 243, 354, 882
BtuMI TCGCGA 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 565, 1306
CciI TCATGA 1 cut(s) 909
CfoI GCGC 2 cut(s) 352, 709
Cfr10I RCCGGY 2 cut(s) 1118, 1385
Cfr13I GGNCC 3 cut(s) 248, 767, 1274
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 713
Csp6I GTAC 6 cut(s) 190, 289, 454, 1164, 1186, 1300
CviQI GTAC 6 cut(s) 190, 289, 454, 1164, 1186, 1300
DdeI CTNAG 8 cut(s) 480, 554, 831, 837, 957, 1180, 1232, 1371
DpnI GATC 4 cut(s) 334, 418, 854, 1382
DpnII GATC 4 cut(s) 332, 416, 852, 1380
DraIII CACNNNGTG 1 cut(s) 1336
EaeI YGGCCR 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 534, 945
Eco147I AGGCCT 2 cut(s) 497, 1097
Eco47I GGWCC 2 cut(s) 248, 767
Eco81I CCTNAGG 2 cut(s) 831, 1371
EcoO109I RGGNCCY 1 cut(s) 767
EcoRII CCWGG 5 cut(s) 100, 421, 667, 988, 1288
EcoT14I CCWWGG 2 cut(s) 534, 945
EcoT22I ATGCAT 2 cut(s) 648, 777
ErhI CCWWGG 2 cut(s) 534, 945
FaqI GGGAC 1 cut(s) 753
FauI CCCGC 1 cut(s) 379
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 9 cut(s) 36, 345, 348, 366, 699, 710, 750, 759, 1070
FokI GGATG 2 cut(s) 193, 655
Fsp4HI GCNGC 9 cut(s) 36, 345, 348, 366, 699, 710, 750, 759, 1070
FspBI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
GlaI GCGC 2 cut(s) 351, 708
GluI GCNGC 9 cut(s) 36, 345, 348, 366, 699, 710, 750, 759, 1070
GsaI CCCAGC 1 cut(s) 1247
HaeIII GGCC 6 cut(s) 118, 497, 539, 931, 1097, 1275
HapII CCGG 6 cut(s) 158, 437, 1119, 1386, 1406, 1421
HgaI GACGC 1 cut(s) 713
HhaI GCGC 2 cut(s) 352, 709
Hin6I GCGC 2 cut(s) 350, 707
HinP1I GCGC 2 cut(s) 350, 707
HinfI GANTC 7 cut(s) 131, 470, 570, 590, 733, 977, 1062
HpaII CCGG 6 cut(s) 158, 437, 1119, 1386, 1406, 1421
HphI GGTGA 3 cut(s) 140, 460, 1375
Hpy166II GTNNAC 4 cut(s) 257, 529, 1171, 1330
Hpy188I TCNGA 6 cut(s) 79, 305, 595, 1091, 1134, 1183
Hpy8I GTNNAC 4 cut(s) 257, 529, 1171, 1330
HpyAV CCTTC 7 cut(s) 104, 396, 487, 500, 739, 1129, 1138
HpyCH4III ACNGT 4 cut(s) 637, 886, 1159, 1168
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 5 cut(s) 236, 319, 567, 646, 775
HpyF3I CTNAG 8 cut(s) 480, 554, 831, 837, 957, 1180, 1232, 1371
HpySE526I ACGT 1 cut(s) 918
HspAI GCGC 2 cut(s) 350, 707
Kzo9I GATC 4 cut(s) 332, 416, 852, 1380
LmnI GCTCC 3 cut(s) 32, 105, 373
Lsp1109I GCAGC 5 cut(s) 22, 331, 352, 696, 736
LweI GCATC 3 cut(s) 306, 633, 1386
MaeI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 5 cut(s) 631, 786, 806, 1121, 1436
MalI GATC 4 cut(s) 334, 418, 854, 1382
MboI GATC 4 cut(s) 332, 416, 852, 1380
MboII GAAGA 7 cut(s) 196, 410, 565, 754, 898, 1022, 1390
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 963, 1289
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 4 cut(s) 163, 263, 600, 619
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 2 cut(s) 464, 971
MmeI TCCRAC 2 cut(s) 328, 901
Mox20I TGGCCA 1 cut(s) 539
Mph1103I ATGCAT 2 cut(s) 648, 777
MscI TGGCCA 1 cut(s) 539
MslI CAYNNNNRTG 1 cut(s) 797
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 6 cut(s) 158, 437, 1119, 1386, 1406, 1421
MspR9I CCNGG 5 cut(s) 102, 423, 669, 990, 1290
Mva1269I GAATGC 2 cut(s) 299, 646
MvaI CCWGG 5 cut(s) 102, 423, 669, 990, 1290
MvnI CGCG 3 cut(s) 350, 393, 707
NdeII GATC 4 cut(s) 332, 416, 852, 1380
NlaIV GGNNCC 1 cut(s) 769
NmuCI GTSAC 3 cut(s) 786, 806, 1436
NruI TCGCGA 1 cut(s) 393
NsiI ATGCAT 2 cut(s) 648, 777
NspI RCATGY 1 cut(s) 829
PagI TCATGA 1 cut(s) 909
PasI CCCWGGG 2 cut(s) 422, 1289
PceI AGGCCT 2 cut(s) 497, 1097
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 1432
PctI GAATGC 2 cut(s) 299, 646
PfeI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
PkrI GCNGC 9 cut(s) 37, 346, 349, 367, 700, 711, 751, 760, 1071
PleI GAGTC 2 cut(s) 464, 971
PpsI GAGTC 2 cut(s) 464, 971
PpuMI RGGWCCY 1 cut(s) 767
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 767
Psp6I CCWGG 5 cut(s) 100, 421, 667, 988, 1288
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 5 cut(s) 100, 421, 667, 988, 1288
PspN4I GGNNCC 1 cut(s) 769
PspPI GGNCC 3 cut(s) 248, 767, 1274
PspPPI RGGWCCY 1 cut(s) 767
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstI CTGCAG 2 cut(s) 238, 569
PsuI RGATCY 1 cut(s) 416
RruI TCGCGA 1 cut(s) 393
RsaI GTAC 6 cut(s) 191, 290, 455, 1165, 1187, 1301
RsaNI GTAC 6 cut(s) 190, 289, 454, 1164, 1186, 1300
RseI CAYNNNNRTG 1 cut(s) 797
SatI GCNGC 9 cut(s) 36, 345, 348, 366, 699, 710, 750, 759, 1070
Sau3AI GATC 4 cut(s) 332, 416, 852, 1380
Sau96I GGNCC 3 cut(s) 248, 767, 1274
ScaI AGTACT 2 cut(s) 290, 1187
SchI GAGTC 2 cut(s) 464, 971
ScrFI CCNGG 5 cut(s) 102, 423, 669, 990, 1290
SduI GDGCHC 2 cut(s) 963, 1289
SfaNI GCATC 3 cut(s) 306, 633, 1386
SfcI CTRYAG 2 cut(s) 234, 565
SgrAI CRCCGGYG 1 cut(s) 1385
SinI GGWCC 2 cut(s) 248, 767
SmiMI CAYNNNNRTG 1 cut(s) 797
Sse9I AATT 4 cut(s) 163, 263, 600, 619
SseBI AGGCCT 2 cut(s) 497, 1097
SsiI CCGC 6 cut(s) 348, 372, 699, 758, 845, 1070
SspI AATATT 1 cut(s) 1031
SspMI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
StuI AGGCCT 2 cut(s) 497, 1097
StyD4I CCNGG 5 cut(s) 100, 421, 667, 988, 1288
StyI CCWWGG 2 cut(s) 534, 945
TaaI ACNGT 4 cut(s) 637, 886, 1159, 1168
TaiI ACGT 1 cut(s) 921
TaqI TCGA 5 cut(s) 138, 314, 331, 522, 585
TasI AATT 4 cut(s) 163, 263, 600, 619
TatI WGTACW 3 cut(s) 288, 1163, 1185
TauI GCSGC 4 cut(s) 350, 701, 761, 1072
TfiI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
TscAI CASTG 3 cut(s) 243, 361, 889
TseFI GTSAC 3 cut(s) 786, 806, 1436
TseI GCWGC 5 cut(s) 35, 344, 365, 709, 749
Tsp45I GTSAC 3 cut(s) 786, 806, 1436
TspDTI ATGAA 9 cut(s) 294, 411, 506, 898, 926, 1023, 1040, 1065, 1190
TspRI CASTG 3 cut(s) 243, 361, 889
VpaK11BI GGWCC 2 cut(s) 248, 767
XapI RAATTY 2 cut(s) 163, 263
XceI RCATGY 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
ZrmI AGTACT 2 cut(s) 290, 1187
Zsp2I ATGCAT 2 cut(s) 648, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.