RLG00000032375

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002150
Physical Location & Seq
Reverse (-)
14130126 .. 14132162
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032375

Sequence Viewer

Length: 1137 bp
ATGGAGACCCTTTACCTTGTTCTCTCTTTGGGTGCTGCTCTTTTCGCCTTTGCCCTCTTCGCATTCAAATCTGAAGATGGCAAGAACCTCCCACCAGGCAGCATGGGGTGGCCTATTGTGGGAGAAACTCTCGAGTTTCTTTTTGGCAAGCCGGAAAACTTTGTGTTCAAGAGGATGAAGAGGTACTCCCCTGACATCTTCAAGACCAAGATTCTTGGGGAGAAAACCGCTGTCATTTGTGGACCTAATGGACACAAATTTCTCTTCACCAATGAGCAAAAATACTTCACTGCATTTCGACCACACTCCATGCAGAAGATGTTCAGGTCATACAAGGCTGCTGCAGCTGCTGCTCCTGCTCCGAAACAGCCTGCTCGCGACGAAGAGGCGAAAGTACTAAGGTCACCGGGGTTCCTTAAGCCAGAAGCATTGATGAGGTACTTGGGGAAAATGGACTCAATCACCCAAGAACAGATGAAGGTCTACTGGGAAGGCAAGAATCAGGTCTTGGCCTACCCTTTGGCCAAGACTTTGACTCTTAGTCTTGCCTGCAGATTCTTCTTGGGGATTGATGAGCCGGAGAGGATTGCTAGGCTTGTGGAGAACTTTGATGATGTGACTGTAGGGATGCACTCACTTATTGTGAACTTCCCAGGAACCATTTTCTACAGAGCAACAAAAGCAGCTGATGCGATCAGGAAGGAGTTGAAGACTGTGATCCAGGAGAAGAAGGCTGCAATGGCATCAGGAGCACCCATGATGGATATTCTGACACATATGATTGTGGCTAGTGACCCATCTGGGCAACACATGCCTGAGGCTGAGATTGCAGATAAGATGATGGGTTTGTTGACTGCTGGGTATAGTACTGTGGCTACTGCTATGACTTTCTTCATGAAGTATGTGGGAGAAAGGTCTGACATCTATGCCAATGTCCTAGCAGAACAAATGGAGGTTGCAGAGTCAAAAAAGCCTGGACAACTTTTGGAATGGGATGACTTGAACAAGATGAAGTACTCATGGAATGTCATCTATGAGGTTATGAGATTCACTCCACCACTTCAGGGAACATTCAGAGAGGCCTTGACCGATTTCGAGTATGCCGGTTACACCATTCCCAAGGGCTGGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

379

Amino Acids

42.62

Weight (kDa)

8.86

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 378 2.2e-32 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1125
AccI GTMKAC 1 cut(s) 483
AccII CGCG 1 cut(s) 378
AciI CCGC 1 cut(s) 228
AclWI GGATC 1 cut(s) 712
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 2 cut(s) 93, 1046
AfaI GTAC 5 cut(s) 185, 396, 440, 868, 1016
AfiI CCNNNNNNNGG 3 cut(s) 119, 1064, 1125
AflII CTTAAG 1 cut(s) 416
AgsI TTSAA 5 cut(s) 67, 169, 202, 709, 1003
AhdI GACNNNNNGTC 1 cut(s) 540
AjnI CCWGG 4 cut(s) 94, 652, 720, 973
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 2 cut(s) 347, 686
AluI AGCT 2 cut(s) 347, 686
Alw21I GWGCWC 1 cut(s) 754
AlwI GGATC 1 cut(s) 712
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 4 cut(s) 110, 510, 522, 1080
ApeKI GCWGC 9 cut(s) 35, 99, 338, 341, 344, 347, 350, 683, 734
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 3 cut(s) 259, 396, 454
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 816
BalI TGGCCA 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 9 cut(s) 22, 111, 325, 328, 334, 337, 356, 695, 721
BccI CCATC 4 cut(s) 71, 754, 805, 835
BciT130I CCWGG 4 cut(s) 96, 654, 722, 975
BcnI CCSGG 1 cut(s) 408
BfaI CTAG 3 cut(s) 591, 789, 938
BfmI CTRYAG 4 cut(s) 342, 550, 621, 667
BfrI CTTAAG 1 cut(s) 416
BisI GCNGC 9 cut(s) 36, 100, 339, 342, 345, 348, 351, 684, 735
BlsI GCNGC 9 cut(s) 37, 101, 340, 343, 346, 349, 352, 685, 736
BmcAI AGTACT 3 cut(s) 396, 868, 1016
Bme1390I CCNGG 5 cut(s) 96, 408, 654, 722, 975
Bme18I GGWCC 1 cut(s) 242
BmeRI GACNNNNNGTC 1 cut(s) 540
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 413, 658
BmrFI CCNGG 5 cut(s) 96, 408, 654, 722, 975
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 3 cut(s) 618, 679, 752
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 716
BplI GAGNNNNNCTC 4 cut(s) 114, 146, 1036, 1068
BpuMI CCSGG 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 407, 652, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 1064, 1125
Bse118I RCCGGY 1 cut(s) 1103
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 816
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 4 cut(s) 96, 654, 722, 975
BseDI CCNNGG 3 cut(s) 407, 652, 1119
BseGI GGATG 3 cut(s) 180, 633, 1000
BseLI CCNNNNNNNGG 3 cut(s) 119, 1064, 1125
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 807, 813
BseNI ACTGG 1 cut(s) 491
BseXI GCAGC 9 cut(s) 22, 111, 325, 328, 334, 337, 356, 695, 721
BseYI CCCAGC 1 cut(s) 857
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 4 cut(s) 112, 512, 524, 1082
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 4 cut(s) 152, 407, 578, 1104
BslI CCNNNNNNNGG 3 cut(s) 119, 1064, 1125
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 4 cut(s) 112, 512, 524, 1082
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 2 cut(s) 693, 717
Bsp68I TCGCGA 1 cut(s) 378
BspACI CCGC 1 cut(s) 228
BspANI GGCC 4 cut(s) 112, 512, 524, 1082
BspCNI CTCAG 2 cut(s) 808, 814
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 2 cut(s) 413, 658
BspMAI CTGCAG 2 cut(s) 346, 554
BspPI GGATC 1 cut(s) 712
BspTI CTTAAG 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 1103
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 1 cut(s) 1103
BssECI CCNNGG 3 cut(s) 407, 652, 1119
BssMI GATC 2 cut(s) 693, 717
BssT1I CCWWGG 1 cut(s) 1119
Bst2UI CCWGG 4 cut(s) 96, 654, 722, 975
Bst4CI ACNGT 3 cut(s) 622, 715, 871
Bst6I CTCTTC 4 cut(s) 62, 173, 269, 378
BstAFI CTTAAG 1 cut(s) 416
BstAPI GCANNNNNTGC 3 cut(s) 350, 689, 811
BstC8I GCNNGC 4 cut(s) 149, 372, 376, 550
BstDEI CTNAG 4 cut(s) 398, 539, 816, 822
BstEII GGTNACC 1 cut(s) 402
BstF5I GGATG 3 cut(s) 180, 633, 1000
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 2 cut(s) 696, 720
BstMBI GATC 2 cut(s) 693, 717
BstNI CCWGG 4 cut(s) 96, 654, 722, 975
BstNSI RCATGY 1 cut(s) 814
BstPI GGTNACC 1 cut(s) 402
BstSCI CCNGG 5 cut(s) 94, 406, 652, 720, 973
BstSFI CTRYAG 4 cut(s) 342, 550, 621, 667
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 9 cut(s) 22, 111, 325, 328, 334, 337, 356, 695, 721
BstV2I GAAGAC 1 cut(s) 716
Bsu36I CCTNAGG 1 cut(s) 816
BsuRI GGCC 4 cut(s) 112, 512, 524, 1082
BtsCI GGATG 3 cut(s) 180, 633, 1000
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
BtuMI TCGCGA 1 cut(s) 378
Cac8I GCNNGC 4 cut(s) 149, 372, 376, 550
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 894
Cfr10I RCCGGY 1 cut(s) 1103
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 5 cut(s) 184, 395, 439, 867, 1015
CviAII CATG 6 cut(s) 103, 310, 757, 811, 895, 1020
CviQI GTAC 5 cut(s) 184, 395, 439, 867, 1015
DdeI CTNAG 4 cut(s) 398, 539, 816, 822
DpnI GATC 2 cut(s) 695, 719
DpnII GATC 2 cut(s) 693, 717
DriI GACNNNNNGTC 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 4 cut(s) 62, 173, 269, 378
Eam1105I GACNNNNNGTC 1 cut(s) 540
EarI CTCTTC 4 cut(s) 62, 173, 269, 378
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 1 cut(s) 1082
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 93, 1046
Eco81I CCTNAGG 1 cut(s) 816
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 402
EcoRII CCWGG 4 cut(s) 94, 652, 720, 973
EcoT14I CCWWGG 1 cut(s) 1119
ErhI CCWWGG 1 cut(s) 1119
FaeI CATG 6 cut(s) 106, 313, 760, 814, 898, 1023
FatI CATG 6 cut(s) 102, 309, 756, 810, 894, 1019
FauNDI CATATG 1 cut(s) 777
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 9 cut(s) 36, 100, 339, 342, 345, 348, 351, 684, 735
FokI GGATG 3 cut(s) 187, 640, 1007
Fsp4HI GCNGC 9 cut(s) 36, 100, 339, 342, 345, 348, 351, 684, 735
FspBI CTAG 3 cut(s) 591, 789, 938
GluI GCNGC 9 cut(s) 36, 100, 339, 342, 345, 348, 351, 684, 735
GsaI CCCAGC 1 cut(s) 861
HaeIII GGCC 4 cut(s) 112, 512, 524, 1082
HapII CCGG 4 cut(s) 152, 407, 578, 1104
Hin1II CATG 6 cut(s) 106, 313, 760, 814, 898, 1023
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 7 cut(s) 211, 455, 499, 535, 555, 962, 1047
HpaII CCGG 4 cut(s) 152, 407, 578, 1104
HphI GGTGA 3 cut(s) 259, 396, 454
Hpy166II GTNNAC 4 cut(s) 242, 484, 646, 852
Hpy188I TCNGA 5 cut(s) 73, 363, 771, 919, 1076
Hpy188III TCNNGA 7 cut(s) 131, 169, 202, 377, 697, 747, 895
Hpy8I GTNNAC 4 cut(s) 242, 484, 646, 852
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 5 cut(s) 472, 485, 694, 724, 1123
HpyCH4III ACNGT 3 cut(s) 622, 715, 871
HpyCH4V TGCA 8 cut(s) 293, 313, 344, 552, 631, 737, 830, 959
HpyF3I CTNAG 4 cut(s) 398, 539, 816, 822
Hsp92II CATG 6 cut(s) 106, 313, 760, 814, 898, 1023
Kzo9I GATC 2 cut(s) 693, 717
LmnI GCTCC 3 cut(s) 358, 364, 749
Lsp1109I GCAGC 9 cut(s) 22, 111, 325, 328, 334, 337, 356, 695, 721
LweI GCATC 3 cut(s) 618, 679, 752
MaeI CTAG 3 cut(s) 591, 789, 938
MaeIII GTNAC 4 cut(s) 402, 616, 791, 1106
MalI GATC 2 cut(s) 695, 719
MboI GATC 2 cut(s) 693, 717
MhlI GDGCHC 1 cut(s) 754
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 3 cut(s) 449, 529, 971
Mox20I TGGCCA 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 320
MscI TGGCCA 1 cut(s) 524
MseI TTAA 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 3 cut(s) 230, 347, 686
MspCI CTTAAG 1 cut(s) 416
MspI CCGG 4 cut(s) 152, 407, 578, 1104
MspR9I CCNGG 5 cut(s) 96, 408, 654, 722, 975
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 4 cut(s) 96, 654, 722, 975
MvnI CGCG 1 cut(s) 378
NciI CCSGG 1 cut(s) 408
NdeI CATATG 1 cut(s) 777
NdeII GATC 2 cut(s) 693, 717
NlaIII CATG 6 cut(s) 106, 313, 760, 814, 898, 1023
NlaIV GGNNCC 2 cut(s) 413, 658
NmuCI GTSAC 3 cut(s) 402, 616, 791
NruI TCGCGA 1 cut(s) 378
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 894
PceI AGGCCT 1 cut(s) 1082
PctI GAATGC 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 4 cut(s) 211, 499, 555, 1047
PflMI CCANNNNNTGG 1 cut(s) 1125
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 9 cut(s) 37, 101, 340, 343, 346, 349, 352, 685, 736
PleI GAGTC 3 cut(s) 449, 529, 970
PpsI GAGTC 3 cut(s) 449, 529, 970
Psp6I CCWGG 4 cut(s) 94, 652, 720, 973
PspEI GGTNACC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 4 cut(s) 94, 652, 720, 973
PspN4I GGNNCC 2 cut(s) 413, 658
PspPI GGNCC 1 cut(s) 242
PstI CTGCAG 2 cut(s) 346, 554
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 2 cut(s) 347, 686
RruI TCGCGA 1 cut(s) 378
RsaI GTAC 5 cut(s) 185, 396, 440, 868, 1016
RsaNI GTAC 5 cut(s) 184, 395, 439, 867, 1015
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 9 cut(s) 36, 100, 339, 342, 345, 348, 351, 684, 735
Sau3AI GATC 2 cut(s) 693, 717
Sau96I GGNCC 1 cut(s) 242
ScaI AGTACT 3 cut(s) 396, 868, 1016
SchI GAGTC 3 cut(s) 449, 529, 971
ScrFI CCNGG 5 cut(s) 96, 408, 654, 722, 975
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 3 cut(s) 618, 679, 752
SfcI CTRYAG 4 cut(s) 342, 550, 621, 667
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 131
SmlI CTYRAG 2 cut(s) 131, 416
SmoI CTYRAG 2 cut(s) 131, 416
Sse9I AATT 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 1082
SsiI CCGC 1 cut(s) 228
SspMI CTAG 3 cut(s) 591, 789, 938
StuI AGGCCT 1 cut(s) 1082
StyD4I CCNGG 5 cut(s) 94, 406, 652, 720, 973
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 3 cut(s) 622, 715, 871
TaqI TCGA 3 cut(s) 132, 298, 1095
TaqII GACCGA 1 cut(s) 1103
TasI AATT 1 cut(s) 257
TatI WGTACW 3 cut(s) 394, 866, 1014
TfiI GAWTC 4 cut(s) 211, 499, 555, 1047
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 3 cut(s) 402, 616, 791
TseI GCWGC 9 cut(s) 35, 99, 338, 341, 344, 347, 350, 683, 734
Tsp45I GTSAC 3 cut(s) 402, 616, 791
TspDTI ATGAA 5 cut(s) 191, 491, 883, 911, 1025
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 1125
Vha464I CTTAAG 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 3 cut(s) 591, 789, 938
ZrmI AGTACT 3 cut(s) 396, 868, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.