MD10G1240600.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002150
Physical Location & Seq
Reverse (-)
33605650 .. 33607690
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1240600.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGAAACCCTGTACCTTGTTTTATCTTTGGGAGCTGCTTTATTAGCTTTTACCATCTTTGCATTCAAGGGCAAATCAGACGATGGCAAAAACCTTCCACCAGGGAGCATGGGGTGGCCTATTGTGGGTGAATCAATCGAGTTTTTGTTCGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGAAGGTACTCTCCTGAAATCTTCAAGACCTATATTCTTGGAGAGAAAACTGCAGTTATTTGTGGTCCTAGTGGACACAAATTCCTGTTCTCCAATGAACAAAAGTACTTCACAGCTTTTCGACCACATTCGATGCAAAAGATGTTTCGATCATACAAGGCTGCGGCCCCCACTGCTCCTGCTGTGGCACAACCTTCTCGTGATGAAGAAGCGAAAGTAATAAGATCTCCAGGGTTTCTGAAGCCGGAAGCATTGGTGAGGTACTTGGGGAGAATGGACTCTATCACTCAGGAACAGATGAAGGTCTATTGGGAAGGAAAAGATGAGGTCCAGGTCTACCCCTTGGCCAAGACCCTCACTCTAGGTCTTGCTTGCAGATTCTTCCTGGGTATCGACGAACCCGAGCGAATTGCAAGGCTTGTGAGCAATTTTGACGATGTTACCGTTGGGATGCATTCACTTATTATCAACTTCCCAGGAACAACATTCTACAAGGCAACTAAAGCGGCCGATGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCAATGGCCGCAGGAGGTCCCATGCATGATATATTGTCACACATGATTGCGGCTAGTGACCCATCTGGCAAACTCATGCCTGAGGCTGAGGTTGCAGATAAGATCATGGGTTTGCTCACAGCGGGATATAGCACTGTGGCTACTGCCATGACTTTCTTCATGAAATATGTTGGAGAGAGGCCAGACATCTATGCCAAGGTTCTAGCTGAGCAAATGGAGATTGCAAACTCCAAGAAGCCAGGAGACTTTTTGGAATGGGAAGACATCAACAAAATGAAGTACTCATGGAACGTGTTGTATGAAGTGATGAGATTCACTCCACCACTTCAGGGGACATTCAGAGAAGCCTTGACTGATTTCACCTATGCTGGTTACACCATCCCAAAGGGCTGGAAGGTCTATTGGACTGTTAGCACAACAAACATGAACCCAGAGTACTTTCCCAACCCAGAAAAGTTTGACCCATCAAGATACGATGACCTAAATGCATTCCCAGCATTCACATTCGTTCCATTTGGAGGAGGGCCAAGAATGTGCCCAGGCAAAGAGTACGCTCGCCTAGCAATTCTCACTTTCGTTCACAACGTGGTCAAGAGGTTCAAATGGGAAGTGTTATTTCCTAAGGAGAAGATCACCGGTGACATGATGCCAACACCGGAGAAAGGACTTCCAGTTCGCCTTACCCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

478

Amino Acids

53.99

Weight (kDa)

9.09

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 452 6.8e-59 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 519
AciI CCGC 5 cut(s) 347, 689, 750, 791, 863
AcoI YGGCCR 3 cut(s) 528, 690, 747
AcsI RAATTY 2 cut(s) 163, 263
AcuI CTGAAG 2 cut(s) 443, 1052
AdeI CACNNNGTG 1 cut(s) 1327
AfaI GTAC 7 cut(s) 14, 191, 290, 446, 1022, 1178, 1292
AfiI CCNNNNNNNGG 4 cut(s) 125, 1070, 1259, 1403
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 1 cut(s) 1376
AgsI TTSAA 4 cut(s) 67, 175, 208, 1342
AjnI CCWGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
AloI GAACNNNNNNTCC 2 cut(s) 1398, 1430
AluBI AGCT 4 cut(s) 35, 47, 299, 947
AluI AGCT 4 cut(s) 35, 47, 299, 947
Alw26I GTCTC 1 cut(s) 978
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 7 cut(s) 116, 348, 528, 690, 747, 920, 1265
ApeKI GCWGC 4 cut(s) 35, 344, 700, 740
ApoI RAATTY 2 cut(s) 163, 263
ArsI GACNNNNNNTTYG 2 cut(s) 1398, 1430
AsiGI ACCGGT 1 cut(s) 1376
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 5 cut(s) 248, 349, 511, 758, 1265
AsuHPI GGTGA 5 cut(s) 140, 451, 1093, 1366, 1391
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 3 cut(s) 248, 511, 758
AxyI CCTNAGG 2 cut(s) 822, 1362
BaeGI GKGCMC 1 cut(s) 1280
BalI TGGCCA 1 cut(s) 530
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 1008
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 4 cut(s) 22, 331, 687, 727
BccI CCATC 5 cut(s) 62, 77, 811, 1127, 1213
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
BcoDI GTCTC 1 cut(s) 978
BfaI CTAG 6 cut(s) 252, 545, 795, 944, 1301, 1432
BfmI CTRYAG 1 cut(s) 234
BglII AGATCT 1 cut(s) 407
BisI GCNGC 8 cut(s) 36, 345, 348, 690, 701, 741, 750, 792
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 8 cut(s) 37, 346, 349, 691, 702, 742, 751, 793
BmcAI AGTACT 3 cut(s) 290, 1022, 1178
Bme1390I CCNGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
Bme18I GGWCC 3 cut(s) 248, 511, 758
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 5 cut(s) 248, 349, 511, 758, 1265
BmiI GGNNCC 2 cut(s) 351, 760
BmrFI CCNGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
BmsI GCATC 4 cut(s) 306, 624, 685, 1377
BpiI GAAGAC 1 cut(s) 1008
BplI GAGNNNNNCTC 2 cut(s) 1042, 1074
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 828
Bpu1102I GCTNAGC 1 cut(s) 948
BsaJI CCNNGG 7 cut(s) 101, 413, 525, 568, 658, 936, 1279
BsaWI WCCGGW 2 cut(s) 1376, 1396
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 1070, 1259, 1403
Bse118I RCCGGY 1 cut(s) 1376
Bse1I ACTGG 1 cut(s) 1412
Bse21I CCTNAGG 2 cut(s) 822, 1362
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
BseDI CCNNGG 7 cut(s) 101, 413, 525, 568, 658, 936, 1279
BseGI GGATG 3 cut(s) 186, 639, 1119
BseLI CCNNNNNNNGG 4 cut(s) 125, 1070, 1259, 1403
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 4 cut(s) 485, 813, 819, 939
BseNI ACTGG 1 cut(s) 1412
BseRI GAGGAG 1 cut(s) 1275
BseSI GKGCMC 1 cut(s) 1280
BseX3I CGGCCG 1 cut(s) 690
BseXI GCAGC 4 cut(s) 22, 331, 687, 727
BseYI CCCAGC 1 cut(s) 1234
Bsh1285I CGRYCG 1 cut(s) 693
BshFI GGCC 7 cut(s) 118, 350, 530, 692, 749, 922, 1267
BshTI ACCGGT 1 cut(s) 1376
BsiEI CGRYCG 1 cut(s) 693
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 4 cut(s) 158, 428, 1377, 1397
BslFI GGGAC 2 cut(s) 744, 1087
BslI CCNNNNNNNGG 4 cut(s) 125, 1070, 1259, 1403
BsmAI GTCTC 1 cut(s) 978
BsmFI GGGAC 2 cut(s) 744, 1087
BsmI GAATGC 4 cut(s) 62, 637, 1229, 1238
BsnI GGCC 7 cut(s) 118, 350, 530, 692, 749, 922, 1267
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 1280
Bsp143I GATC 4 cut(s) 332, 407, 843, 1371
Bsp1720I GCTNAGC 1 cut(s) 948
BspACI CCGC 5 cut(s) 347, 689, 750, 791, 863
BspANI GGCC 7 cut(s) 118, 350, 530, 692, 749, 922, 1267
BspCNI CTCAG 4 cut(s) 484, 814, 820, 940
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 2 cut(s) 351, 760
BspMAI CTGCAG 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 1376
BsrI ACTGG 1 cut(s) 1412
BssAI RCCGGY 1 cut(s) 1376
BssECI CCNNGG 7 cut(s) 101, 413, 525, 568, 658, 936, 1279
BssMI GATC 4 cut(s) 332, 407, 843, 1371
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 525, 936
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
Bst4CI ACNGT 3 cut(s) 628, 877, 1150
BstC8I GCNNGC 2 cut(s) 556, 1297
BstDEI CTNAG 5 cut(s) 471, 822, 828, 948, 1362
BstF5I GGATG 3 cut(s) 186, 639, 1119
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 4 cut(s) 335, 410, 846, 1374
BstMAI GTCTC 1 cut(s) 978
BstMBI GATC 4 cut(s) 332, 407, 843, 1371
BstMCI CGRYCG 1 cut(s) 693
BstMWI GCNNNNNNNGC 9 cut(s) 44, 356, 686, 695, 746, 749, 833, 1235, 1301
BstNI CCWGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
BstSCI CCNGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 1280
BstV1I GCAGC 4 cut(s) 22, 331, 687, 727
BstV2I GAAGAC 1 cut(s) 1008
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 1 cut(s) 1131
BstYI RGATCY 1 cut(s) 407
BstZI CGGCCG 1 cut(s) 690
Bsu36I CCTNAGG 2 cut(s) 822, 1362
BsuRI GGCC 7 cut(s) 118, 350, 530, 692, 749, 922, 1267
BtsCI GGATG 3 cut(s) 186, 639, 1119
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 354, 873
Cac8I GCNNGC 2 cut(s) 556, 1297
CciI TCATGA 1 cut(s) 900
CfoI GCGC 1 cut(s) 700
Cfr10I RCCGGY 1 cut(s) 1376
Cfr13I GGNCC 5 cut(s) 248, 349, 511, 758, 1265
Csp6I GTAC 7 cut(s) 13, 190, 289, 445, 1021, 1177, 1291
CspAI ACCGGT 1 cut(s) 1376
CviQI GTAC 7 cut(s) 13, 190, 289, 445, 1021, 1177, 1291
DdeI CTNAG 5 cut(s) 471, 822, 828, 948, 1362
DpnI GATC 4 cut(s) 334, 409, 845, 1373
DpnII GATC 4 cut(s) 332, 407, 843, 1371
DraIII CACNNNGTG 1 cut(s) 1327
EaeI YGGCCR 3 cut(s) 528, 690, 747
EagI CGGCCG 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 525, 936
Eco47I GGWCC 3 cut(s) 248, 511, 758
Eco52I CGGCCG 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 443, 1052
Eco81I CCTNAGG 2 cut(s) 822, 1362
Eco88I CYCGRG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 758
EcoRII CCWGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
EcoT14I CCWWGG 2 cut(s) 525, 936
EcoT22I ATGCAT 3 cut(s) 639, 768, 1231
ErhI CCWWGG 2 cut(s) 525, 936
FaqI GGGAC 2 cut(s) 744, 1087
FauI CCCGC 1 cut(s) 856
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 8 cut(s) 36, 345, 348, 690, 701, 741, 750, 792
FokI GGATG 3 cut(s) 193, 646, 1106
Fsp4HI GCNGC 8 cut(s) 36, 345, 348, 690, 701, 741, 750, 792
FspBI CTAG 6 cut(s) 252, 545, 795, 944, 1301, 1432
GlaI GCGC 1 cut(s) 699
GluI GCNGC 8 cut(s) 36, 345, 348, 690, 701, 741, 750, 792
GsaI CCCAGC 1 cut(s) 1238
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 7 cut(s) 118, 350, 530, 692, 749, 922, 1267
HapII CCGG 4 cut(s) 158, 428, 1377, 1397
HhaI GCGC 1 cut(s) 700
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HinfI GANTC 5 cut(s) 131, 461, 561, 724, 1053
HpaII CCGG 4 cut(s) 158, 428, 1377, 1397
HphI GGTGA 5 cut(s) 140, 451, 1093, 1366, 1391
Hpy166II GTNNAC 3 cut(s) 257, 520, 1321
Hpy188I TCNGA 3 cut(s) 79, 423, 1082
Hpy8I GTNNAC 3 cut(s) 257, 520, 1321
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 7 cut(s) 104, 180, 387, 478, 491, 730, 1129
HpyCH4III ACNGT 3 cut(s) 628, 877, 1150
HpyCH4IV ACGT 2 cut(s) 1032, 1326
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 356, 686, 695, 746, 749, 833, 1235, 1301
HpyF3I CTNAG 5 cut(s) 471, 822, 828, 948, 1362
HpySE526I ACGT 2 cut(s) 1032, 1326
HspAI GCGC 1 cut(s) 698
Kzo9I GATC 4 cut(s) 332, 407, 843, 1371
LmnI GCTCC 3 cut(s) 32, 105, 364
Lsp1109I GCAGC 4 cut(s) 22, 331, 687, 727
LweI GCATC 4 cut(s) 306, 624, 685, 1377
MaeI CTAG 6 cut(s) 252, 545, 795, 944, 1301, 1432
MaeII ACGT 2 cut(s) 1032, 1326
MaeIII GTNAC 6 cut(s) 622, 777, 797, 1112, 1379, 1427
MalI GATC 4 cut(s) 334, 409, 845, 1373
MboI GATC 4 cut(s) 332, 407, 843, 1371
MboII GAAGA 7 cut(s) 196, 401, 556, 745, 889, 1013, 1381
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 1280
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 5 cut(s) 163, 263, 591, 610, 1305
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 892
Mox20I TGGCCA 1 cut(s) 530
Mph1103I ATGCAT 3 cut(s) 639, 768, 1231
MscI TGGCCA 1 cut(s) 530
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 4 cut(s) 158, 428, 1377, 1397
MspR9I CCNGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
Mva1269I GAATGC 4 cut(s) 62, 637, 1229, 1238
MvaI CCWGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
MwoI GCNNNNNNNGC 9 cut(s) 44, 356, 686, 695, 746, 749, 833, 1235, 1301
NdeII GATC 4 cut(s) 332, 407, 843, 1371
NlaIV GGNNCC 2 cut(s) 351, 760
NmuCI GTSAC 4 cut(s) 777, 797, 1379, 1427
NsiI ATGCAT 3 cut(s) 639, 768, 1231
PagI TCATGA 1 cut(s) 900
PcsI WCGNNNNNNNCGW 4 cut(s) 582, 624, 1323, 1423
PctI GAATGC 4 cut(s) 62, 637, 1229, 1238
PfeI GAWTC 4 cut(s) 131, 561, 724, 1053
PinAI ACCGGT 1 cut(s) 1376
PkrI GCNGC 8 cut(s) 37, 346, 349, 691, 702, 742, 751, 793
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
PpuMI RGGWCCY 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 758
Psp6I CCWGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
PspFI CCCAGC 1 cut(s) 1234
PspGI CCWGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
PspN4I GGNNCC 2 cut(s) 351, 760
PspPI GGNCC 5 cut(s) 248, 349, 511, 758, 1265
PspPPI RGGWCCY 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 1160, 1192
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 7 cut(s) 14, 191, 290, 446, 1022, 1178, 1292
RsaNI GTAC 7 cut(s) 13, 190, 289, 445, 1021, 1177, 1291
SatI GCNGC 8 cut(s) 36, 345, 348, 690, 701, 741, 750, 792
Sau3AI GATC 4 cut(s) 332, 407, 843, 1371
Sau96I GGNCC 5 cut(s) 248, 349, 511, 758, 1265
ScaI AGTACT 3 cut(s) 290, 1022, 1178
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 7 cut(s) 102, 414, 515, 569, 660, 981, 1281
SduI GDGCHC 1 cut(s) 1280
SfaNI GCATC 4 cut(s) 306, 624, 685, 1377
SfcI CTRYAG 1 cut(s) 234
SgrAI CRCCGGYG 1 cut(s) 1376
SinI GGWCC 3 cut(s) 248, 511, 758
Sse9I AATT 5 cut(s) 163, 263, 591, 610, 1305
SsiI CCGC 5 cut(s) 347, 689, 750, 791, 863
SspMI CTAG 6 cut(s) 252, 545, 795, 944, 1301, 1432
StyD4I CCNGG 7 cut(s) 100, 412, 513, 567, 658, 979, 1279
StyI CCWWGG 2 cut(s) 525, 936
TaaI ACNGT 3 cut(s) 628, 877, 1150
TaiI ACGT 2 cut(s) 1035, 1329
TaqI TCGA 5 cut(s) 138, 304, 314, 331, 576
TasI AATT 5 cut(s) 163, 263, 591, 610, 1305
TatI WGTACW 3 cut(s) 288, 1020, 1176
TauI GCSGC 4 cut(s) 350, 692, 752, 794
TfiI GAWTC 4 cut(s) 131, 561, 724, 1053
TscAI CASTG 2 cut(s) 361, 880
TseFI GTSAC 4 cut(s) 777, 797, 1379, 1427
TseI GCWGC 4 cut(s) 35, 344, 700, 740
Tsp45I GTSAC 4 cut(s) 777, 797, 1379, 1427
TspDTI ATGAA 8 cut(s) 294, 402, 497, 889, 917, 1031, 1056, 1181
TspRI CASTG 2 cut(s) 361, 880
VpaK11BI GGWCC 3 cut(s) 248, 511, 758
XapI RAATTY 2 cut(s) 163, 263
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 6 cut(s) 252, 545, 795, 944, 1301, 1432
ZrmI AGTACT 3 cut(s) 290, 1022, 1178
Zsp2I ATGCAT 3 cut(s) 639, 768, 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.