Rw5G012060

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002150
Physical Location & Seq
Forward (+)
14110460 .. 14112393
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012060.1

Sequence Viewer

Length: 1428 bp
ATGGAGACCCTTTACCTTGTTCTATCTTTAGGTGCTGCAGTCTTCGCCTTTGCCCTCTTCGCATTCAAATCCGAAGATGGCAAGAACCTCCCACCAGGAAGCATGGGGTGGCCTATTGTGGGAGAAACTCTTGAGTTTCTTTTTGGCAAGCCGGAAAACTTTGTGTTCAAGAGGATGAAGCGGTACTCCCCTGACATCTTCAAGACCAAGATTCTTGGGGAGAAAACCGCTGTCATTTGTGGACCGAATGGACACAAGTTTCTCTTCACCAATGAGCAAAAATACTTCACTGCATTTCGACCACACTCCATGCAGAAGATGTTCAGGTCATACAAGGCTGCTGCAGCTGCTGCTCCTGCTCCGAAACAGCCTGCTCGCGACGAAGAGGCGAAAGTACTAAGGTCACCGGGTTTTCTGAAGGCGGAAGCACTGATGAGGTACTTGGGGAAAATGGACTCAATCACACAAGAACAAATGAAGATCAACTGGGAAGGCAAGAACCAGGTCTTGGCTTACCCTCTGGCCAAGACTCTGACTCTAAGTCTTGCCTGTAGATTCTTCTTGGGGATTGATGAGCCGGAGAGGATTGCTAGGCTTGTGGAGAACTTTGATGATGTGACTGTGGGGATGCACTCACTTATTGTGAACTTCCCGGGAACCATTTTCTACAGAGCAACAAAAGCAGCTGATGCGATCAGGAAGGAGTTGAAGACTGTGATCCAGGAGAAGAAGGCTGCAATGGCATCAGGAGCACCCATGATGGATATTCTGACACATATGATTGTGGCCAGTGACCCATCTGGGCAACACATGCCTGAGGCTGAGATTGCAGATAAGATGATGGGCTTGTTGACTGCTGGGTATAGTACTGTGGCTACTGCTATGACTTTCTTCATGAAGTATGTGGGAGAAAGGCCTGACATCTATGCCAAGGTCCTAGCAGAACAAATGGAGGTGGCAGAGTCAAAGAAGCCAGGACAACTTTTGGAATGGGATGACTTGAACAAGATGAAGTACTCCTGGAATGTCATATATGAGGTGATGAGGTTCACCCCGCCACTTCAGGGAACATTCCGAGAGGCCTTGACCGATTTTGAATATGCCGGTTACACCATTCCTAAGGGCTGGAAGGTATATTGGACTGTTAGCACAACAAACATGAACCCGGACTACTTCCCCAACCCGGAAAAGTTTGACCCCTCGAGATACGATGATGTAAGCGCATTCCCACCATACACATTTGTCCCATTTGGAGGAGGACCAAGAATGTGCCCTGGAAAAGAGTACGCTAGGCTTGCAATACTCACTTTTGTTCACAATGTGGTCAAGAGGTTCAAGTGGGAAGTGGTATTTCCCAAAGAAAAGATCACGGGTGATATGATGCCAACACCAGAAAAAGGACTTCCCATTCGCCTTACACGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

475

Amino Acids

53.83

Weight (kDa)

9.08

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 450 6.8e-63 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 508
AccII CGCG 1 cut(s) 378
AciI CCGC 4 cut(s) 181, 228, 422, 1055
AclWI GGATC 1 cut(s) 712
AcoI YGGCCR 2 cut(s) 522, 786
AcuI CTGAAG 2 cut(s) 437, 1046
AdeI CACNNNGTG 1 cut(s) 1321
AfaI GTAC 6 cut(s) 185, 396, 440, 868, 1016, 1286
AfiI CCNNNNNNNGG 6 cut(s) 119, 508, 1064, 1183, 1253, 1397
AflIII ACRYGT 1 cut(s) 1418
AgsI TTSAA 7 cut(s) 67, 169, 202, 709, 1003, 1097, 1336
AhdI GACNNNNNGTC 1 cut(s) 540
AjiI CACGTC 1 cut(s) 1421
AjnI CCWGG 6 cut(s) 94, 501, 720, 973, 1019, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 2 cut(s) 347, 686
AluI AGCT 2 cut(s) 347, 686
Alw21I GWGCWC 1 cut(s) 754
AlwI GGATC 1 cut(s) 712
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 2 cut(s) 652, 1201
AoxI GGCC 5 cut(s) 110, 522, 786, 914, 1080
ApeKI GCWGC 8 cut(s) 35, 338, 341, 344, 347, 350, 683, 734
ArsI GACNNNNNNTTYG 2 cut(s) 1392, 1424
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 3 cut(s) 242, 934, 1259
AsuC2I CCSGG 5 cut(s) 408, 653, 654, 1166, 1184
AsuHPI GGTGA 5 cut(s) 259, 396, 1042, 1051, 1385
AvaI CYCGRG 2 cut(s) 652, 1201
AvaII GGWCC 3 cut(s) 242, 934, 1259
AxyI CCTNAGG 2 cut(s) 816, 1119
BaeGI GKGCMC 1 cut(s) 1274
BalI TGGCCA 2 cut(s) 524, 788
BbsI GAAGAC 2 cut(s) 34, 716
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 8 cut(s) 22, 325, 328, 334, 337, 356, 695, 721
BccI CCATC 4 cut(s) 71, 754, 805, 835
BciT130I CCWGG 6 cut(s) 96, 503, 722, 975, 1021, 1275
BcnI CCSGG 5 cut(s) 408, 653, 654, 1166, 1184
BfaI CTAG 4 cut(s) 591, 938, 1290, 1426
BfmI CTRYAG 4 cut(s) 36, 342, 550, 667
BisI GCNGC 8 cut(s) 36, 339, 342, 345, 348, 351, 684, 735
BlsI GCNGC 8 cut(s) 37, 340, 343, 346, 349, 352, 685, 736
BmcAI AGTACT 3 cut(s) 396, 868, 1016
Bme18I GGWCC 3 cut(s) 242, 934, 1259
BmeRI GACNNNNNGTC 1 cut(s) 540
BmeT110I CYCGRG 2 cut(s) 652, 1201
BmgBI CACGTC 1 cut(s) 1421
BmgT120I GGNCC 3 cut(s) 242, 934, 1259
BmiI GGNNCC 1 cut(s) 658
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 4 cut(s) 618, 679, 752, 1371
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 2 cut(s) 34, 716
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 5 cut(s) 408, 653, 654, 1166, 1184
BsaJI CCNNGG 3 cut(s) 652, 930, 1273
Bsc4I CCNNNNNNNGG 6 cut(s) 119, 508, 1064, 1183, 1253, 1397
Bse118I RCCGGY 1 cut(s) 1103
Bse1I ACTGG 2 cut(s) 491, 789
Bse21I CCTNAGG 2 cut(s) 816, 1119
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 6 cut(s) 96, 503, 722, 975, 1021, 1275
BseDI CCNNGG 3 cut(s) 652, 930, 1273
BseGI GGATG 3 cut(s) 180, 633, 1000
BseLI CCNNNNNNNGG 6 cut(s) 119, 508, 1064, 1183, 1253, 1397
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 807, 813
BseNI ACTGG 2 cut(s) 491, 789
BseRI GAGGAG 1 cut(s) 1269
BseSI GKGCMC 1 cut(s) 1274
BseXI GCAGC 8 cut(s) 22, 325, 328, 334, 337, 356, 695, 721
BseYI CCCAGC 1 cut(s) 857
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 5 cut(s) 112, 524, 788, 916, 1082
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 2 cut(s) 652, 1201
BsiSI CCGG 7 cut(s) 152, 407, 578, 653, 1104, 1166, 1184
BslFI GGGAC 1 cut(s) 1229
BslI CCNNNNNNNGG 6 cut(s) 119, 508, 1064, 1183, 1253, 1397
BsmFI GGGAC 1 cut(s) 1229
BsmI GAATGC 2 cut(s) 62, 1223
BsnI GGCC 5 cut(s) 112, 524, 788, 916, 1082
BsoBI CYCGRG 2 cut(s) 652, 1201
Bsp1286I GDGCHC 2 cut(s) 754, 1274
Bsp143I GATC 4 cut(s) 480, 693, 717, 1365
Bsp68I TCGCGA 1 cut(s) 378
BspACI CCGC 4 cut(s) 181, 228, 422, 1055
BspANI GGCC 5 cut(s) 112, 524, 788, 916, 1082
BspCNI CTCAG 2 cut(s) 808, 814
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 1 cut(s) 658
BspMAI CTGCAG 2 cut(s) 40, 346
BspPI GGATC 1 cut(s) 712
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 1103
BsrI ACTGG 2 cut(s) 491, 789
BssAI RCCGGY 1 cut(s) 1103
BssECI CCNNGG 3 cut(s) 652, 930, 1273
BssMI GATC 4 cut(s) 480, 693, 717, 1365
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 6 cut(s) 96, 503, 722, 975, 1021, 1275
Bst4CI ACNGT 4 cut(s) 622, 715, 871, 1144
Bst6I CTCTTC 3 cut(s) 62, 269, 378
BstAPI GCANNNNNTGC 3 cut(s) 350, 689, 811
BstC8I GCNNGC 4 cut(s) 149, 372, 376, 1296
BstDEI CTNAG 5 cut(s) 398, 539, 816, 822, 1119
BstEII GGTNACC 1 cut(s) 402
BstF5I GGATG 3 cut(s) 180, 633, 1000
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 1223
BstKTI GATC 4 cut(s) 483, 696, 720, 1368
BstMBI GATC 4 cut(s) 480, 693, 717, 1365
BstNI CCWGG 6 cut(s) 96, 503, 722, 975, 1021, 1275
BstNSI RCATGY 1 cut(s) 814
BstPI GGTNACC 1 cut(s) 402
BstSFI CTRYAG 4 cut(s) 36, 342, 550, 667
BstSLI GKGCMC 1 cut(s) 1274
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 8 cut(s) 22, 325, 328, 334, 337, 356, 695, 721
BstV2I GAAGAC 2 cut(s) 34, 716
Bsu36I CCTNAGG 2 cut(s) 816, 1119
BsuRI GGCC 5 cut(s) 112, 524, 788, 916, 1082
BtrI CACGTC 1 cut(s) 1421
BtsCI GGATG 3 cut(s) 180, 633, 1000
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 3 cut(s) 288, 428, 796
BtuMI TCGCGA 1 cut(s) 378
Cac8I GCNNGC 4 cut(s) 149, 372, 376, 1296
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 894
CfoI GCGC 1 cut(s) 1223
Cfr10I RCCGGY 1 cut(s) 1103
Cfr13I GGNCC 3 cut(s) 242, 934, 1259
Cfr9I CCCGGG 1 cut(s) 652
CsiI ACCWGGT 1 cut(s) 501
Csp6I GTAC 6 cut(s) 184, 395, 439, 867, 1015, 1285
CviAII CATG 6 cut(s) 103, 310, 757, 811, 895, 1159
CviQI GTAC 6 cut(s) 184, 395, 439, 867, 1015, 1285
DdeI CTNAG 5 cut(s) 398, 539, 816, 822, 1119
DpnI GATC 4 cut(s) 482, 695, 719, 1367
DpnII GATC 4 cut(s) 480, 693, 717, 1365
DraIII CACNNNGTG 1 cut(s) 1321
DriI GACNNNNNGTC 1 cut(s) 540
EaeI YGGCCR 2 cut(s) 522, 786
Eam1104I CTCTTC 3 cut(s) 62, 269, 378
Eam1105I GACNNNNNGTC 1 cut(s) 540
EarI CTCTTC 3 cut(s) 62, 269, 378
EciI GGCGGA 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 930
Eco147I AGGCCT 2 cut(s) 916, 1082
Eco47I GGWCC 3 cut(s) 242, 934, 1259
Eco57I CTGAAG 2 cut(s) 437, 1046
Eco81I CCTNAGG 2 cut(s) 816, 1119
Eco88I CYCGRG 2 cut(s) 652, 1201
Eco91I GGTNACC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 934
EcoO65I GGTNACC 1 cut(s) 402
EcoRII CCWGG 6 cut(s) 94, 501, 720, 973, 1019, 1273
EcoT14I CCWWGG 1 cut(s) 930
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 6 cut(s) 106, 313, 760, 814, 898, 1162
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 1 cut(s) 1229
FatI CATG 6 cut(s) 102, 309, 756, 810, 894, 1158
FauI CCCGC 1 cut(s) 1062
FauNDI CATATG 1 cut(s) 777
Fnu4HI GCNGC 8 cut(s) 36, 339, 342, 345, 348, 351, 684, 735
FokI GGATG 3 cut(s) 187, 640, 1007
Fsp4HI GCNGC 8 cut(s) 36, 339, 342, 345, 348, 351, 684, 735
FspBI CTAG 4 cut(s) 591, 938, 1290, 1426
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 8 cut(s) 36, 339, 342, 345, 348, 351, 684, 735
GsaI CCCAGC 1 cut(s) 861
HaeIII GGCC 5 cut(s) 112, 524, 788, 916, 1082
HapII CCGG 7 cut(s) 152, 407, 578, 653, 1104, 1166, 1184
HhaI GCGC 1 cut(s) 1223
Hin1II CATG 6 cut(s) 106, 313, 760, 814, 898, 1162
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 6 cut(s) 211, 455, 529, 535, 555, 962
HpaII CCGG 7 cut(s) 152, 407, 578, 653, 1104, 1166, 1184
HphI GGTGA 5 cut(s) 259, 396, 1042, 1051, 1385
Hpy166II GTNNAC 5 cut(s) 242, 646, 852, 1050, 1315
Hpy188I TCNGA 6 cut(s) 73, 363, 417, 534, 771, 1076
Hpy188III TCNNGA 9 cut(s) 131, 169, 202, 377, 697, 747, 895, 1203, 1327
Hpy8I GTNNAC 5 cut(s) 242, 646, 852, 1050, 1315
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 5 cut(s) 412, 485, 694, 724, 1123
HpyCH4III ACNGT 4 cut(s) 622, 715, 871, 1144
HpyCH4IV ACGT 1 cut(s) 1420
HpyCH4V TGCA 8 cut(s) 38, 293, 313, 344, 631, 737, 830, 1298
HpyF3I CTNAG 5 cut(s) 398, 539, 816, 822, 1119
HpySE526I ACGT 1 cut(s) 1420
Hsp92II CATG 6 cut(s) 106, 313, 760, 814, 898, 1162
HspAI GCGC 1 cut(s) 1221
Kzo9I GATC 4 cut(s) 480, 693, 717, 1365
LmnI GCTCC 3 cut(s) 358, 364, 749
Lsp1109I GCAGC 8 cut(s) 22, 325, 328, 334, 337, 356, 695, 721
LweI GCATC 4 cut(s) 618, 679, 752, 1371
MabI ACCWGGT 1 cut(s) 501
MaeI CTAG 4 cut(s) 591, 938, 1290, 1426
MaeII ACGT 1 cut(s) 1420
MaeIII GTNAC 5 cut(s) 402, 616, 791, 1106, 1421
MalI GATC 4 cut(s) 482, 695, 719, 1367
MboI GATC 4 cut(s) 480, 693, 717, 1365
MhlI GDGCHC 2 cut(s) 754, 1274
MlsI TGGCCA 2 cut(s) 524, 788
MluNI TGGCCA 2 cut(s) 524, 788
MlyI GAGTC 4 cut(s) 449, 523, 529, 971
Mox20I TGGCCA 2 cut(s) 524, 788
MroXI GAANNNNTTC 1 cut(s) 320
MscI TGGCCA 2 cut(s) 524, 788
Msp20I TGGCCA 2 cut(s) 524, 788
MspA1I CMGCKG 3 cut(s) 230, 347, 686
MspI CCGG 7 cut(s) 152, 407, 578, 653, 1104, 1166, 1184
Mva1269I GAATGC 2 cut(s) 62, 1223
MvaI CCWGG 6 cut(s) 96, 503, 722, 975, 1021, 1275
MvnI CGCG 1 cut(s) 378
NciI CCSGG 5 cut(s) 408, 653, 654, 1166, 1184
NdeI CATATG 1 cut(s) 777
NdeII GATC 4 cut(s) 480, 693, 717, 1365
NlaIII CATG 6 cut(s) 106, 313, 760, 814, 898, 1162
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 4 cut(s) 402, 616, 791, 1421
NruI TCGCGA 1 cut(s) 378
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 1201
PagI TCATGA 1 cut(s) 894
PceI AGGCCT 2 cut(s) 916, 1082
PcsI WCGNNNNNNNCGW 1 cut(s) 1417
PctI GAATGC 2 cut(s) 62, 1223
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 2 cut(s) 211, 555
PflMI CCANNNNNTGG 1 cut(s) 508
PfoI TCCNGGA 2 cut(s) 720, 1019
PkrI GCNGC 8 cut(s) 37, 340, 343, 346, 349, 352, 685, 736
PleI GAGTC 4 cut(s) 449, 523, 529, 970
PpsI GAGTC 4 cut(s) 449, 523, 529, 970
PpuMI RGGWCCY 1 cut(s) 934
Psp5II RGGWCCY 1 cut(s) 934
Psp6I CCWGG 6 cut(s) 94, 501, 720, 973, 1019, 1273
PspEI GGTNACC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 6 cut(s) 94, 501, 720, 973, 1019, 1273
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 3 cut(s) 242, 934, 1259
PspPPI RGGWCCY 1 cut(s) 934
PsrI GAACNNNNNNTAC 2 cut(s) 1154, 1186
PstI CTGCAG 2 cut(s) 40, 346
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 2 cut(s) 347, 686
RruI TCGCGA 1 cut(s) 378
RsaI GTAC 6 cut(s) 185, 396, 440, 868, 1016, 1286
RsaNI GTAC 6 cut(s) 184, 395, 439, 867, 1015, 1285
SatI GCNGC 8 cut(s) 36, 339, 342, 345, 348, 351, 684, 735
Sau3AI GATC 4 cut(s) 480, 693, 717, 1365
Sau96I GGNCC 3 cut(s) 242, 934, 1259
ScaI AGTACT 3 cut(s) 396, 868, 1016
SchI GAGTC 4 cut(s) 449, 523, 529, 971
SduI GDGCHC 2 cut(s) 754, 1274
SexAI ACCWGGT 1 cut(s) 501
SfaNI GCATC 4 cut(s) 618, 679, 752, 1371
SfcI CTRYAG 4 cut(s) 36, 342, 550, 667
Sfr274I CTCGAG 1 cut(s) 1201
SinI GGWCC 3 cut(s) 242, 934, 1259
SlaI CTCGAG 1 cut(s) 1201
SmaI CCCGGG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 131, 1201
SmoI CTYRAG 2 cut(s) 131, 1201
SseBI AGGCCT 2 cut(s) 916, 1082
SsiI CCGC 4 cut(s) 181, 228, 422, 1055
SspMI CTAG 4 cut(s) 591, 938, 1290, 1426
StuI AGGCCT 2 cut(s) 916, 1082
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 4 cut(s) 622, 715, 871, 1144
TaiI ACGT 1 cut(s) 1423
TaqI TCGA 2 cut(s) 298, 1202
TaqII GACCGA 2 cut(s) 259, 1103
TatI WGTACW 3 cut(s) 394, 866, 1014
TfiI GAWTC 2 cut(s) 211, 555
TscAI CASTG 3 cut(s) 295, 435, 796
TseFI GTSAC 4 cut(s) 402, 616, 791, 1421
TseI GCWGC 8 cut(s) 35, 338, 341, 344, 347, 350, 683, 734
Tsp45I GTSAC 4 cut(s) 402, 616, 791, 1421
TspDTI ATGAA 6 cut(s) 191, 491, 883, 911, 1025, 1175
TspMI CCCGGG 1 cut(s) 652
TspRI CASTG 3 cut(s) 295, 435, 796
Van91I CCANNNNNTGG 1 cut(s) 508
VpaK11BI GGWCC 3 cut(s) 242, 934, 1259
XceI RCATGY 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 1201
XmaI CCCGGG 1 cut(s) 652
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 4 cut(s) 591, 938, 1290, 1426
ZrmI AGTACT 3 cut(s) 396, 868, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.