MD05G1262600.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002150
Physical Location & Seq
Reverse (-)
39744110 .. 39745841
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262600.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGGAGACTTTGTACCTTATATTCTCTTTAGGTGCTGCTTTCTTAGCCTTTACAATCTTTGCATTCAAAGGAAAATCAGATGATGGCAAAAACCTCCCACCAGGAAGCATGGGGTGGCCTATTGTGGGTGAATCGATCGAGTTCCTGTTCGGGAAGCCGGAAAATTTTGTGTTCAAGAGGATGAGGAGGTACTCTCCTGACATCTTCAAGACCTATATTCTGGGGGAGAAAACTGCAGTGATTTGTGGTCCTAGTGGACACAAATTTCTGTTCTCCAATGAACAAAAGTACTTCACAGCTTTTCGACCACATTCAATGCAAAAGATGTTTCGATCATACAAGGCTGCCGCCCCCACCGCTGCTGCTGGTCCTGCTGTGGCACAACCTTCTCGTGATGAAGAAGCGAAAGTTATAAGATCGCCAGGGTTTTTGAAGCCGGAAGCACTGGTTAGGTACTTGGGGAAAATGGACTCTATCACTCAGGAACAGATGAAGGCTTATTGGGAAGGCAAAGATGTGGTCGAGGTCTACCCTTTGGCCAAGACCCTCACTCTAAGTCTTGCCTGCAGATTCTTCTTGGGGATCGATGATTCCGAGCGAATTGCAAGGCTGGTGAGCAACTTTGATGATGTTACTGTTGGTATGCATTCACTTATTATCAACTTCCCAGGAACAACATTCTACAAGGCAACCAAAGCGGCCGATGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCAATGGCCGCAGGAGGTCCCATGCATGATATATTGTCACACATGATCGTGGCTAGTGATCCATCTGGCAAACACATGCCCGAGGCTGAGGTCGCGGACAAGATCATGGGCTTATTGACTGCAGGATACAGCACTGTGGCCACCGCCATGACATTCTTCATGAAATATGTTGGAGAGAGGCCAGACATTTATGCCAAGGTCCTAGCTGAGCACAAGCAGATTGCAGACTCAAAGAAGCCAGGAGACTTTTTGGAATGGGAAGACATCAACAAAATGAAGTACTCATGGAATGTGTTGTATGAAGTGATGAGATTCACGCCGCCACTTCAAGGAACATTCAGAGAGGCCTTGACTGATTTCACCTACGCCGGTTACACCATTCCGAAGGGCTGGAAAGTATATTGGACTGTGAGTACAGTGAACATGAACTCAGAGTACTTCCCCAACCCAGAAATGTTTGACCCATCAAGATATGATGACCTAAGTGTATTCCCAGCTTTCACATTTGTTCCATTTGGAGGGGGGCCAAGAATGTGCCCCGGGAAAGAGTACGCTCGCCTAGCGATACTCACTTTCGTTCACAATGTGGTCAAGAGGTTCGAATGGGAAGTTCTATTTCCTAAGGAGAAGATCACCGGCGACATGATGCCAACACCCGAGAAAGGGCTTCCAGTTCGCCTTCGATGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

481

Amino Acids

54.23

Weight (kDa)

8.9

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 455 8.8e-59 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 845
AciI CCGC 7 cut(s) 348, 357, 698, 759, 845, 894, 1070
AclWI GGATC 2 cut(s) 590, 803
AcoI YGGCCR 4 cut(s) 537, 699, 756, 888
AcsI RAATTY 2 cut(s) 163, 263
AdeI CACNNNGTG 1 cut(s) 1336
AfaI GTAC 8 cut(s) 14, 191, 290, 455, 1031, 1165, 1187, 1301
AfiI CCNNNNNNNGG 5 cut(s) 125, 1079, 1268, 1412, 1413
AgsI TTSAA 6 cut(s) 67, 175, 208, 315, 433, 1079
AjnI CCWGG 4 cut(s) 100, 421, 667, 988
AluBI AGCT 3 cut(s) 299, 956, 1247
AluI AGCT 3 cut(s) 299, 956, 1247
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 987
AlwI GGATC 2 cut(s) 590, 803
Ama87I CYCGRG 3 cut(s) 830, 1289, 1406
AoxI GGCC 8 cut(s) 116, 537, 699, 756, 888, 929, 1095, 1274
ApeKI GCWGC 6 cut(s) 35, 344, 359, 362, 709, 749
ApoI RAATTY 2 cut(s) 163, 263
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 5 cut(s) 248, 368, 767, 949, 1274
AsuC2I CCSGG 2 cut(s) 1290, 1291
AsuHPI GGTGA 4 cut(s) 140, 625, 1102, 1375
AsuII TTCGAA 1 cut(s) 1350
AvaI CYCGRG 3 cut(s) 830, 1289, 1406
AvaII GGWCC 4 cut(s) 248, 368, 767, 949
AxyI CCTNAGG 1 cut(s) 1371
BaeGI GKGCMC 1 cut(s) 1289
BalI TGGCCA 2 cut(s) 539, 890
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 6 cut(s) 22, 331, 346, 349, 696, 736
BccI CCATC 3 cut(s) 77, 820, 1222
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 4 cut(s) 102, 423, 669, 990
BciVI GTATCC 1 cut(s) 869
BcnI CCSGG 2 cut(s) 1290, 1291
BcoDI GTCTC 1 cut(s) 987
BfaI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
BfmI CTRYAG 3 cut(s) 234, 565, 870
BfuI GTATCC 1 cut(s) 869
BlpI GCTNAGC 1 cut(s) 957
BmcAI AGTACT 3 cut(s) 290, 1031, 1187
Bme1390I CCNGG 6 cut(s) 102, 423, 669, 990, 1290, 1291
Bme18I GGWCC 4 cut(s) 248, 368, 767, 949
BmeT110I CYCGRG 3 cut(s) 830, 1289, 1406
BmgT120I GGNCC 5 cut(s) 248, 368, 767, 949, 1274
BmiI GGNNCC 2 cut(s) 769, 1275
BmrFI CCNGG 6 cut(s) 102, 423, 669, 990, 1290, 1291
BmsI GCATC 2 cut(s) 694, 1386
BpiI GAAGAC 1 cut(s) 1017
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 1 cut(s) 837
Bpu1102I GCTNAGC 1 cut(s) 957
Bpu14I TTCGAA 1 cut(s) 1350
BpuMI CCSGG 2 cut(s) 1290, 1291
Bsa29I ATCGAT 2 cut(s) 134, 585
BsaJI CCNNGG 6 cut(s) 422, 667, 831, 945, 1288, 1289
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 1079, 1268, 1412, 1413
Bse118I RCCGGY 2 cut(s) 1118, 1385
Bse1I ACTGG 2 cut(s) 450, 1421
Bse21I CCTNAGG 1 cut(s) 1371
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 4 cut(s) 102, 423, 669, 990
BseCI ATCGAT 2 cut(s) 134, 585
BseDI CCNNGG 6 cut(s) 422, 667, 831, 945, 1288, 1289
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 5 cut(s) 125, 1079, 1268, 1412, 1413
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 4 cut(s) 494, 828, 948, 1194
BseNI ACTGG 2 cut(s) 450, 1421
BseRI GAGGAG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 1289
BseX3I CGGCCG 1 cut(s) 699
BseXI GCAGC 6 cut(s) 22, 331, 346, 349, 696, 736
BseYI CCCAGC 1 cut(s) 1243
Bsh1236I CGCG 1 cut(s) 845
Bsh1285I CGRYCG 2 cut(s) 138, 702
BshFI GGCC 8 cut(s) 118, 539, 701, 758, 890, 931, 1097, 1276
BshVI ATCGAT 2 cut(s) 134, 585
BsiEI CGRYCG 2 cut(s) 138, 702
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 3 cut(s) 830, 1289, 1406
BsiSI CCGG 5 cut(s) 158, 437, 1119, 1290, 1386
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 5 cut(s) 125, 1079, 1268, 1412, 1413
BsmAI GTCTC 1 cut(s) 987
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 2 cut(s) 62, 646
BsnI GGCC 8 cut(s) 118, 539, 701, 758, 890, 931, 1097, 1276
BsoBI CYCGRG 3 cut(s) 830, 1289, 1406
Bsp119I TTCGAA 1 cut(s) 1350
Bsp1286I GDGCHC 2 cut(s) 963, 1289
Bsp143I GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
Bsp1720I GCTNAGC 1 cut(s) 957
BspACI CCGC 7 cut(s) 348, 357, 698, 759, 845, 894, 1070
BspANI GGCC 8 cut(s) 118, 539, 701, 758, 890, 931, 1097, 1276
BspCNI CTCAG 4 cut(s) 493, 829, 949, 1193
BspDI ATCGAT 2 cut(s) 134, 585
BspFNI CGCG 1 cut(s) 845
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 2 cut(s) 769, 1275
BspMAI CTGCAG 3 cut(s) 238, 569, 874
BspPI GGATC 2 cut(s) 590, 803
BspT104I TTCGAA 1 cut(s) 1350
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 1118, 1385
BsrI ACTGG 2 cut(s) 450, 1421
BssAI RCCGGY 2 cut(s) 1118, 1385
BssECI CCNNGG 6 cut(s) 422, 667, 831, 945, 1288, 1289
BssMI GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 4 cut(s) 102, 423, 669, 990
Bst4CI ACNGT 4 cut(s) 637, 886, 1159, 1168
BstBI TTCGAA 1 cut(s) 1350
BstC8I GCNNGC 2 cut(s) 565, 1306
BstDEI CTNAG 8 cut(s) 43, 480, 554, 837, 957, 1180, 1232, 1371
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 1 cut(s) 845
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 8 cut(s) 138, 335, 419, 585, 798, 811, 855, 1383
BstMAI GTCTC 1 cut(s) 987
BstMBI GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
BstMCI CGRYCG 2 cut(s) 138, 702
BstMWI GCNNNNNNNGC 9 cut(s) 44, 356, 371, 695, 704, 755, 758, 842, 1310
BstNI CCWGG 4 cut(s) 102, 423, 669, 990
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 6 cut(s) 100, 421, 667, 988, 1288, 1289
BstSFI CTRYAG 3 cut(s) 234, 565, 870
BstSLI GKGCMC 1 cut(s) 1289
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 6 cut(s) 22, 331, 346, 349, 696, 736
BstV2I GAAGAC 1 cut(s) 1017
BstZI CGGCCG 1 cut(s) 699
Bsu15I ATCGAT 2 cut(s) 134, 585
Bsu36I CCTNAGG 1 cut(s) 1371
BsuI GTATCC 1 cut(s) 869
BsuRI GGCC 8 cut(s) 118, 539, 701, 758, 890, 931, 1097, 1276
BsuTUI ATCGAT 2 cut(s) 134, 585
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 4 cut(s) 243, 443, 882, 1173
Cac8I GCNNGC 2 cut(s) 565, 1306
CciI TCATGA 1 cut(s) 909
CfoI GCGC 1 cut(s) 709
Cfr10I RCCGGY 2 cut(s) 1118, 1385
Cfr13I GGNCC 5 cut(s) 248, 368, 767, 949, 1274
Cfr9I CCCGGG 1 cut(s) 1289
ClaI ATCGAT 2 cut(s) 134, 585
Csp6I GTAC 8 cut(s) 13, 190, 289, 454, 1030, 1164, 1186, 1300
CviQI GTAC 8 cut(s) 13, 190, 289, 454, 1030, 1164, 1186, 1300
DdeI CTNAG 8 cut(s) 43, 480, 554, 837, 957, 1180, 1232, 1371
DpnI GATC 8 cut(s) 137, 334, 418, 584, 797, 810, 854, 1382
DpnII GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
DraIII CACNNNGTG 1 cut(s) 1336
EaeI YGGCCR 4 cut(s) 537, 699, 756, 888
EagI CGGCCG 1 cut(s) 699
EclXI CGGCCG 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 945
Eco147I AGGCCT 1 cut(s) 1097
Eco47I GGWCC 4 cut(s) 248, 368, 767, 949
Eco52I CGGCCG 1 cut(s) 699
Eco81I CCTNAGG 1 cut(s) 1371
Eco88I CYCGRG 3 cut(s) 830, 1289, 1406
EcoO109I RGGNCCY 2 cut(s) 767, 949
EcoRII CCWGG 4 cut(s) 100, 421, 667, 988
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 2 cut(s) 648, 777
ErhI CCWWGG 1 cut(s) 945
FaqI GGGAC 1 cut(s) 753
FblI GTMKAC 1 cut(s) 528
FokI GGATG 1 cut(s) 193
FspBI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
GlaI GCGC 1 cut(s) 708
GsaI CCCAGC 1 cut(s) 1247
HaeIII GGCC 8 cut(s) 118, 539, 701, 758, 890, 931, 1097, 1276
HapII CCGG 5 cut(s) 158, 437, 1119, 1290, 1386
HhaI GCGC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 7 cut(s) 131, 470, 570, 590, 733, 977, 1062
HpaII CCGG 5 cut(s) 158, 437, 1119, 1290, 1386
HphI GGTGA 4 cut(s) 140, 625, 1102, 1375
Hpy166II GTNNAC 4 cut(s) 257, 529, 1171, 1330
Hpy188I TCNGA 5 cut(s) 79, 595, 1091, 1134, 1183
Hpy8I GTNNAC 4 cut(s) 257, 529, 1171, 1330
HpyAV CCTTC 6 cut(s) 396, 487, 500, 739, 1129, 1439
HpyCH4III ACNGT 4 cut(s) 637, 886, 1159, 1168
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 356, 371, 695, 704, 755, 758, 842, 1310
HpyF3I CTNAG 8 cut(s) 43, 480, 554, 837, 957, 1180, 1232, 1371
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
Lsp1109I GCAGC 6 cut(s) 22, 331, 346, 349, 696, 736
LweI GCATC 2 cut(s) 694, 1386
MaeI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
MaeIII GTNAC 4 cut(s) 631, 786, 1121, 1436
MalI GATC 8 cut(s) 137, 334, 418, 584, 797, 810, 854, 1382
MboI GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
MboII GAAGA 7 cut(s) 196, 410, 565, 754, 898, 1022, 1390
MhlI GDGCHC 2 cut(s) 963, 1289
MlsI TGGCCA 2 cut(s) 539, 890
MluCI AATT 3 cut(s) 163, 263, 600
MluNI TGGCCA 2 cut(s) 539, 890
MlyI GAGTC 2 cut(s) 464, 971
MmeI TCCRAC 1 cut(s) 901
Mox20I TGGCCA 2 cut(s) 539, 890
Mph1103I ATGCAT 2 cut(s) 648, 777
MscI TGGCCA 2 cut(s) 539, 890
MslI CAYNNNNRTG 2 cut(s) 797, 896
Msp20I TGGCCA 2 cut(s) 539, 890
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 5 cut(s) 158, 437, 1119, 1290, 1386
MspR9I CCNGG 6 cut(s) 102, 423, 669, 990, 1290, 1291
Mva1269I GAATGC 2 cut(s) 62, 646
MvaI CCWGG 4 cut(s) 102, 423, 669, 990
MvnI CGCG 1 cut(s) 845
MwoI GCNNNNNNNGC 9 cut(s) 44, 356, 371, 695, 704, 755, 758, 842, 1310
NciI CCSGG 2 cut(s) 1290, 1291
NdeII GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
NlaIV GGNNCC 2 cut(s) 769, 1275
NmuCI GTSAC 2 cut(s) 786, 1436
NsiI ATGCAT 2 cut(s) 648, 777
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1350
PagI TCATGA 1 cut(s) 909
PceI AGGCCT 1 cut(s) 1097
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PctI GAATGC 2 cut(s) 62, 646
PfeI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 2 cut(s) 464, 971
PpsI GAGTC 2 cut(s) 464, 971
PpuMI RGGWCCY 2 cut(s) 767, 949
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 2 cut(s) 767, 949
Psp6I CCWGG 4 cut(s) 100, 421, 667, 988
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 4 cut(s) 100, 421, 667, 988
PspN4I GGNNCC 2 cut(s) 769, 1275
PspPI GGNCC 5 cut(s) 248, 368, 767, 949, 1274
PspPPI RGGWCCY 2 cut(s) 767, 949
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstI CTGCAG 3 cut(s) 238, 569, 874
PvuI CGATCG 1 cut(s) 138
RsaI GTAC 8 cut(s) 14, 191, 290, 455, 1031, 1165, 1187, 1301
RsaNI GTAC 8 cut(s) 13, 190, 289, 454, 1030, 1164, 1186, 1300
RseI CAYNNNNRTG 2 cut(s) 797, 896
Sau3AI GATC 8 cut(s) 135, 332, 416, 582, 795, 808, 852, 1380
Sau96I GGNCC 5 cut(s) 248, 368, 767, 949, 1274
ScaI AGTACT 3 cut(s) 290, 1031, 1187
SchI GAGTC 2 cut(s) 464, 971
ScrFI CCNGG 6 cut(s) 102, 423, 669, 990, 1290, 1291
SduI GDGCHC 2 cut(s) 963, 1289
SfaNI GCATC 2 cut(s) 694, 1386
SfcI CTRYAG 3 cut(s) 234, 565, 870
SfuI TTCGAA 1 cut(s) 1350
SgrAI CRCCGGYG 1 cut(s) 1385
SinI GGWCC 4 cut(s) 248, 368, 767, 949
SmaI CCCGGG 1 cut(s) 1291
SmiMI CAYNNNNRTG 2 cut(s) 797, 896
Sse9I AATT 3 cut(s) 163, 263, 600
SseBI AGGCCT 1 cut(s) 1097
SsiI CCGC 7 cut(s) 348, 357, 698, 759, 845, 894, 1070
SspMI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
StuI AGGCCT 1 cut(s) 1097
StyD4I CCNGG 6 cut(s) 100, 421, 667, 988, 1288, 1289
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 4 cut(s) 637, 886, 1159, 1168
TaqI TCGA 8 cut(s) 134, 138, 304, 331, 522, 585, 1350, 1432
TasI AATT 3 cut(s) 163, 263, 600
TatI WGTACW 4 cut(s) 288, 1029, 1163, 1185
TauI GCSGC 4 cut(s) 350, 701, 761, 1072
TfiI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
TscAI CASTG 4 cut(s) 243, 450, 889, 1173
TseFI GTSAC 2 cut(s) 786, 1436
TseI GCWGC 6 cut(s) 35, 344, 359, 362, 709, 749
Tsp45I GTSAC 2 cut(s) 786, 1436
TspDTI ATGAA 8 cut(s) 294, 411, 506, 898, 926, 1040, 1065, 1190
TspMI CCCGGG 1 cut(s) 1289
TspRI CASTG 4 cut(s) 243, 450, 889, 1173
VpaK11BI GGWCC 4 cut(s) 248, 368, 767, 949
XapI RAATTY 2 cut(s) 163, 263
XceI RCATGY 1 cut(s) 829
XmaI CCCGGG 1 cut(s) 1289
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 5 cut(s) 252, 804, 953, 1310, 1441
ZrmI AGTACT 3 cut(s) 290, 1031, 1187
Zsp2I ATGCAT 2 cut(s) 648, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.