pycom05g24610

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002150
Physical Location & Seq
Reverse (-)
26131155 .. 26132697
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24610.1

Sequence Viewer

Length: 1413 bp
ATGGAGACTTTGTACCTTATATTCTCTTTAGGTGCTGCTTTCTTAGCCTTTACAATCTTTGCATTCAAAGGAAAATCAGATGATGGCAAAAATCTCCCACCAGGAAGCATGGGGTGGCCTATTGTGGGTGAATCGATCGAGTTCCTGTTCGGGAAGCCAGAAAATTTTGTGTTCAAGAGGATGAGGAGGTACTCTCCTGACATCTTCAAGACCTATATTCTTGGGGAGAAAACTGCAGTGATTTGTGGTCCTAGTGGACACAAATTTCTGTTCTCCAATGAACAAAAATACTTCACAGCTTTTCGACCACATTCGATGCAAAAGATGTTTCGATCATACAAGGCTGCCGCCCCCACCGCTGCTGCTGCTTCTGATGTGGCACAACCTTCTCGTGATGAAGAAGCGAAAGTTATAAGATCGCCAGGGTTTTTGAAGCCGGAAGCACTGGTTAGGTACTTGGGGAAAATGGACTCTGTCACCCAGGAACAGATGAAGGCCTATTGGGAAGGCAAAGATGTGGTCGAGGTCTACCCTTTGGCCAAGACCCTCACTCTAAGTCTTGCCTGCAGATTCTTCCTGGGGATCGATGATTCCGAGCGAATTGCAAGGCTGGTGAGCAACTTTGATGATGTTACTGTTGGTATGCATTCACTTATTATCAACTTCCCCGGAACAACATTCTACAAGGCAACCAAAGCGGCCAATGCGCTGCGAAAGGAGTTGAGGATTGTGATTCAGGAGAAGAAGGCTGCAATGGCCGCAGGAGGTCCCATGCATGATATATTGTCACACATGATCGTGGCTAGTGATCCATCCGGTAAACACATGCCCGAGGCTGAGGTCGCGGACAAGATCATGGGCTTATTGACCGCAGGATATAGCACTGTGGCCACTGCCATGACATTCTTCATGAAATATGTTGGAGAGAGGCCAGACATTTATGCCAAGGTCCTAGCTGAGCACAAGCAAATTGCAGACTCAAAGAAGCCAGGAGACTTTTTGGAATGGGAAGACATCAACAAAATGAAGTATTCATGGAACGTGTTGTATGAAGTGATGAGATTCACGCCGCCACTCCAAGGAACATTTAGAGAGGCCTTGACTGATTTTACCTACGCCGGTTACACCATTCCGAAGGGCTGGAAAGTATATTGGACTGTGAGTACAGTAAACATGAACTCAGAGTACTTCCCCAGCCCAGAAAAGTTTGACCCATCAAGATATGATGACCTAAGTGCATTCCCAGCTTTCACATTTGTTCCATTTGGAGGGGGGCCAAGAATGTGCCCTGGGAAAGAGTACGCTCGCCTAGCGATACTCACTTTCGTTCACAATGTGGTCAAGAGGTTCAAATGGGAAGTGGTATTTCCTAAGGAGAAACCTTTACTCTTCGATCGATCACCGGCGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

471

Amino Acids

53.05

Weight (kDa)

9.08

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 458 2.9e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 845
AciI CCGC 7 cut(s) 348, 357, 698, 759, 845, 870, 1070
AclWI GGATC 2 cut(s) 590, 803
AcoI YGGCCR 4 cut(s) 537, 699, 756, 888
AcsI RAATTY 2 cut(s) 163, 263
AdeI CACNNNGTG 1 cut(s) 1336
AfaI GTAC 6 cut(s) 14, 191, 455, 1165, 1187, 1301
AfiI CCNNNNNNNGG 3 cut(s) 125, 1079, 1268
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 5 cut(s) 67, 175, 208, 433, 1351
AjnI CCWGG 6 cut(s) 100, 421, 480, 576, 988, 1288
AluBI AGCT 3 cut(s) 299, 956, 1247
AluI AGCT 3 cut(s) 299, 956, 1247
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 987
AlwI GGATC 2 cut(s) 590, 803
Ama87I CYCGRG 1 cut(s) 830
AoxI GGCC 9 cut(s) 116, 495, 537, 699, 756, 888, 929, 1095, 1274
ApeKI GCWGC 7 cut(s) 35, 344, 359, 362, 365, 709, 749
ApoI RAATTY 2 cut(s) 163, 263
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 4 cut(s) 248, 767, 949, 1274
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 4 cut(s) 140, 469, 625, 1392
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 3 cut(s) 248, 767, 949
AxyI CCTNAGG 1 cut(s) 1371
BaeGI GKGCMC 1 cut(s) 1289
BalI TGGCCA 2 cut(s) 539, 890
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 837
BbvI GCAGC 7 cut(s) 22, 331, 346, 349, 352, 696, 736
BccI CCATC 3 cut(s) 77, 820, 1222
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 6 cut(s) 102, 423, 482, 578, 990, 1290
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 1 cut(s) 987
BfaI CTAG 4 cut(s) 252, 804, 953, 1310
BfmI CTRYAG 2 cut(s) 234, 565
BlpI GCTNAGC 1 cut(s) 957
BmcAI AGTACT 1 cut(s) 1187
Bme1390I CCNGG 7 cut(s) 102, 423, 482, 578, 669, 990, 1290
Bme18I GGWCC 3 cut(s) 248, 767, 949
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 4 cut(s) 248, 767, 949, 1274
BmiI GGNNCC 2 cut(s) 769, 1275
BmrFI CCNGG 7 cut(s) 102, 423, 482, 578, 669, 990, 1290
BmsI GCATC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 1017
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 1 cut(s) 837
Bpu1102I GCTNAGC 1 cut(s) 957
BpuMI CCSGG 1 cut(s) 669
Bsa29I ATCGAT 3 cut(s) 134, 585, 1396
BsaJI CCNNGG 9 cut(s) 422, 480, 577, 667, 831, 945, 1078, 1288, 1289
BsaWI WCCGGW 1 cut(s) 815
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 1079, 1268
Bse118I RCCGGY 2 cut(s) 1118, 1402
Bse1I ACTGG 1 cut(s) 450
Bse21I CCTNAGG 1 cut(s) 1371
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 6 cut(s) 102, 423, 482, 578, 990, 1290
BseCI ATCGAT 3 cut(s) 134, 585, 1396
BseDI CCNNGG 9 cut(s) 422, 480, 577, 667, 831, 945, 1078, 1288, 1289
BseGI GGATG 2 cut(s) 186, 812
BseLI CCNNNNNNNGG 3 cut(s) 125, 1079, 1268
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 3 cut(s) 828, 948, 1194
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 1289
BseXI GCAGC 7 cut(s) 22, 331, 346, 349, 352, 696, 736
BseYI CCCAGC 2 cut(s) 1193, 1243
Bsh1236I CGCG 1 cut(s) 845
Bsh1285I CGRYCG 2 cut(s) 138, 1396
BshFI GGCC 9 cut(s) 118, 497, 539, 701, 758, 890, 931, 1097, 1276
BshVI ATCGAT 3 cut(s) 134, 585, 1396
BsiEI CGRYCG 2 cut(s) 138, 1396
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 5 cut(s) 437, 669, 816, 1119, 1403
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 3 cut(s) 125, 1079, 1268
BsmAI GTCTC 1 cut(s) 987
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 3 cut(s) 62, 646, 1238
BsnI GGCC 9 cut(s) 118, 497, 539, 701, 758, 890, 931, 1097, 1276
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 2 cut(s) 963, 1289
Bsp143I GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
Bsp1720I GCTNAGC 1 cut(s) 957
BspACI CCGC 7 cut(s) 348, 357, 698, 759, 845, 870, 1070
BspANI GGCC 9 cut(s) 118, 497, 539, 701, 758, 890, 931, 1097, 1276
BspCNI CTCAG 3 cut(s) 829, 949, 1193
BspDI ATCGAT 3 cut(s) 134, 585, 1396
BspFNI CGCG 1 cut(s) 845
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 2 cut(s) 769, 1275
BspMAI CTGCAG 2 cut(s) 238, 569
BspPI GGATC 2 cut(s) 590, 803
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 1118, 1402
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 2 cut(s) 1118, 1402
BssECI CCNNGG 9 cut(s) 422, 480, 577, 667, 831, 945, 1078, 1288, 1289
BssMI GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 2 cut(s) 945, 1078
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 6 cut(s) 102, 423, 482, 578, 990, 1290
Bst4CI ACNGT 4 cut(s) 637, 886, 1159, 1168
Bst6I CTCTTC 1 cut(s) 1394
BstC8I GCNNGC 2 cut(s) 565, 1306
BstDEI CTNAG 7 cut(s) 43, 554, 837, 957, 1180, 1232, 1371
BstF5I GGATG 2 cut(s) 186, 812
BstFNI CGCG 1 cut(s) 845
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 9 cut(s) 138, 335, 419, 585, 798, 811, 855, 1396, 1400
BstMAI GTCTC 1 cut(s) 987
BstMBI GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
BstMCI CGRYCG 2 cut(s) 138, 1396
BstNI CCWGG 6 cut(s) 102, 423, 482, 578, 990, 1290
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 7 cut(s) 100, 421, 480, 576, 667, 988, 1288
BstSFI CTRYAG 2 cut(s) 234, 565
BstSLI GKGCMC 1 cut(s) 1289
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 7 cut(s) 22, 331, 346, 349, 352, 696, 736
BstV2I GAAGAC 1 cut(s) 1017
Bsu15I ATCGAT 3 cut(s) 134, 585, 1396
Bsu36I CCTNAGG 1 cut(s) 1371
BsuRI GGCC 9 cut(s) 118, 497, 539, 701, 758, 890, 931, 1097, 1276
BsuTUI ATCGAT 3 cut(s) 134, 585, 1396
BtsCI GGATG 2 cut(s) 186, 812
BtsI GCAGTG 2 cut(s) 243, 891
BtsIMutI CAGTG 4 cut(s) 243, 443, 882, 891
Cac8I GCNNGC 2 cut(s) 565, 1306
CciI TCATGA 1 cut(s) 909
CfoI GCGC 1 cut(s) 709
Cfr10I RCCGGY 2 cut(s) 1118, 1402
Cfr13I GGNCC 4 cut(s) 248, 767, 949, 1274
ClaI ATCGAT 3 cut(s) 134, 585, 1396
Csp6I GTAC 6 cut(s) 13, 190, 454, 1164, 1186, 1300
CviQI GTAC 6 cut(s) 13, 190, 454, 1164, 1186, 1300
DdeI CTNAG 7 cut(s) 43, 554, 837, 957, 1180, 1232, 1371
DpnI GATC 9 cut(s) 137, 334, 418, 584, 797, 810, 854, 1395, 1399
DpnII GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
DraIII CACNNNGTG 1 cut(s) 1336
EaeI YGGCCR 4 cut(s) 537, 699, 756, 888
Eam1104I CTCTTC 1 cut(s) 1394
EarI CTCTTC 1 cut(s) 1394
Eco130I CCWWGG 2 cut(s) 945, 1078
Eco147I AGGCCT 2 cut(s) 497, 1097
Eco47I GGWCC 3 cut(s) 248, 767, 949
Eco81I CCTNAGG 1 cut(s) 1371
Eco88I CYCGRG 1 cut(s) 830
EcoO109I RGGNCCY 2 cut(s) 767, 949
EcoRII CCWGG 6 cut(s) 100, 421, 480, 576, 988, 1288
EcoT14I CCWWGG 2 cut(s) 945, 1078
EcoT22I ATGCAT 2 cut(s) 648, 777
ErhI CCWWGG 2 cut(s) 945, 1078
FaqI GGGAC 1 cut(s) 753
FblI GTMKAC 1 cut(s) 528
FokI GGATG 2 cut(s) 193, 799
FspBI CTAG 4 cut(s) 252, 804, 953, 1310
GlaI GCGC 1 cut(s) 708
GsaI CCCAGC 2 cut(s) 1197, 1247
HaeIII GGCC 9 cut(s) 118, 497, 539, 701, 758, 890, 931, 1097, 1276
HapII CCGG 5 cut(s) 437, 669, 816, 1119, 1403
HhaI GCGC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 7 cut(s) 131, 470, 570, 590, 733, 977, 1062
HpaII CCGG 5 cut(s) 437, 669, 816, 1119, 1403
HphI GGTGA 4 cut(s) 140, 469, 625, 1392
Hpy166II GTNNAC 5 cut(s) 257, 529, 821, 1171, 1330
Hpy188I TCNGA 5 cut(s) 79, 373, 595, 1134, 1183
Hpy188III TCNNGA 9 cut(s) 151, 175, 197, 208, 392, 737, 910, 1218, 1342
Hpy8I GTNNAC 5 cut(s) 257, 529, 821, 1171, 1330
HpyAV CCTTC 5 cut(s) 396, 487, 500, 739, 1129
HpyCH4III ACNGT 4 cut(s) 637, 886, 1159, 1168
HpyCH4IV ACGT 1 cut(s) 1041
HpyF3I CTNAG 7 cut(s) 43, 554, 837, 957, 1180, 1232, 1371
HpySE526I ACGT 1 cut(s) 1041
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
Lsp1109I GCAGC 7 cut(s) 22, 331, 346, 349, 352, 696, 736
LweI GCATC 1 cut(s) 306
MaeI CTAG 4 cut(s) 252, 804, 953, 1310
MaeII ACGT 1 cut(s) 1041
MaeIII GTNAC 4 cut(s) 475, 631, 786, 1121
MalI GATC 9 cut(s) 137, 334, 418, 584, 797, 810, 854, 1395, 1399
MboI GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
MboII GAAGA 7 cut(s) 196, 410, 565, 754, 898, 1022, 1381
MhlI GDGCHC 2 cut(s) 963, 1289
MlsI TGGCCA 2 cut(s) 539, 890
MluCI AATT 4 cut(s) 163, 263, 600, 969
MluNI TGGCCA 2 cut(s) 539, 890
MlyI GAGTC 2 cut(s) 464, 971
MmeI TCCRAC 1 cut(s) 901
Mox20I TGGCCA 2 cut(s) 539, 890
Mph1103I ATGCAT 2 cut(s) 648, 777
MscI TGGCCA 2 cut(s) 539, 890
MslI CAYNNNNRTG 2 cut(s) 797, 896
Msp20I TGGCCA 2 cut(s) 539, 890
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 5 cut(s) 437, 669, 816, 1119, 1403
MspR9I CCNGG 7 cut(s) 102, 423, 482, 578, 669, 990, 1290
Mva1269I GAATGC 3 cut(s) 62, 646, 1238
MvaI CCWGG 6 cut(s) 102, 423, 482, 578, 990, 1290
MvnI CGCG 1 cut(s) 845
NciI CCSGG 1 cut(s) 669
NdeII GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
NlaIV GGNNCC 2 cut(s) 769, 1275
NmuCI GTSAC 2 cut(s) 475, 786
NsiI ATGCAT 2 cut(s) 648, 777
NspI RCATGY 1 cut(s) 829
PagI TCATGA 1 cut(s) 909
PasI CCCWGGG 1 cut(s) 1289
PceI AGGCCT 2 cut(s) 497, 1097
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PctI GAATGC 3 cut(s) 62, 646, 1238
PfeI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
PflFI GACNNNGTC 1 cut(s) 473
Ple19I CGATCG 2 cut(s) 138, 1396
PleI GAGTC 2 cut(s) 464, 971
PpsI GAGTC 2 cut(s) 464, 971
PpuMI RGGWCCY 2 cut(s) 767, 949
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 2 cut(s) 767, 949
Psp6I CCWGG 6 cut(s) 100, 421, 480, 576, 988, 1288
PspFI CCCAGC 2 cut(s) 1193, 1243
PspGI CCWGG 6 cut(s) 100, 421, 480, 576, 988, 1288
PspN4I GGNNCC 2 cut(s) 769, 1275
PspPI GGNCC 4 cut(s) 248, 767, 949, 1274
PspPPI RGGWCCY 2 cut(s) 767, 949
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PstI CTGCAG 2 cut(s) 238, 569
PsyI GACNNNGTC 1 cut(s) 473
PvuI CGATCG 2 cut(s) 138, 1396
RsaI GTAC 6 cut(s) 14, 191, 455, 1165, 1187, 1301
RsaNI GTAC 6 cut(s) 13, 190, 454, 1164, 1186, 1300
RseI CAYNNNNRTG 2 cut(s) 797, 896
Sau3AI GATC 9 cut(s) 135, 332, 416, 582, 795, 808, 852, 1393, 1397
Sau96I GGNCC 4 cut(s) 248, 767, 949, 1274
ScaI AGTACT 1 cut(s) 1187
SchI GAGTC 2 cut(s) 464, 971
ScrFI CCNGG 7 cut(s) 102, 423, 482, 578, 669, 990, 1290
SduI GDGCHC 2 cut(s) 963, 1289
SfaNI GCATC 1 cut(s) 306
SfcI CTRYAG 2 cut(s) 234, 565
SgrAI CRCCGGYG 1 cut(s) 1402
SinI GGWCC 3 cut(s) 248, 767, 949
SmiMI CAYNNNNRTG 2 cut(s) 797, 896
Sse9I AATT 4 cut(s) 163, 263, 600, 969
SseBI AGGCCT 2 cut(s) 497, 1097
SsiI CCGC 7 cut(s) 348, 357, 698, 759, 845, 870, 1070
SspMI CTAG 4 cut(s) 252, 804, 953, 1310
StuI AGGCCT 2 cut(s) 497, 1097
StyD4I CCNGG 7 cut(s) 100, 421, 480, 576, 667, 988, 1288
StyI CCWWGG 2 cut(s) 945, 1078
TaaI ACNGT 4 cut(s) 637, 886, 1159, 1168
TaiI ACGT 1 cut(s) 1044
TaqI TCGA 9 cut(s) 134, 138, 304, 314, 331, 522, 585, 1392, 1396
TasI AATT 4 cut(s) 163, 263, 600, 969
TatI WGTACW 2 cut(s) 1163, 1185
TauI GCSGC 4 cut(s) 350, 701, 761, 1072
TfiI GAWTC 5 cut(s) 131, 570, 590, 733, 1062
TscAI CASTG 4 cut(s) 243, 450, 889, 898
TseFI GTSAC 2 cut(s) 475, 786
TseI GCWGC 7 cut(s) 35, 344, 359, 362, 365, 709, 749
Tsp45I GTSAC 2 cut(s) 475, 786
TspDTI ATGAA 9 cut(s) 294, 411, 506, 898, 926, 1023, 1040, 1065, 1190
TspRI CASTG 4 cut(s) 243, 450, 889, 898
Tth111I GACNNNGTC 1 cut(s) 473
VpaK11BI GGWCC 3 cut(s) 248, 767, 949
XapI RAATTY 2 cut(s) 163, 263
XceI RCATGY 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 4 cut(s) 252, 804, 953, 1310
ZrmI AGTACT 1 cut(s) 1187
Zsp2I ATGCAT 2 cut(s) 648, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.