Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003564

Physical Location & Seq

Genomic Coordinates Forward (+)

40390434 .. 40392020

Gene Structure 1587 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1268400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGCTTCCAATGTTTTCAATTTTTTCTCTTTATCTTTTCCTTGTGCTATCAGCTTCCTGGGCGCATCCTCCACCTTCAACACAAGAAAATTTTATCCAATGCCTCTCACGTTATTCTAAGACTCCCATTCCATTCTCTTCAGACTTTCTCACTCCGAAAAATTCTAGCTTCACCGCTGTTCTTGAGTCTTCTGCACAAAACCTCCGGTACTTGGTCTCCTCAGTGCCAAAGCCGGAGTTTATTTTCACACCGGTGCATGATTCTCATGTGCAAGCTGCTGTCATTTGCGCGAAAAAGCTTCGAATCCATCTCAGGGTCCGAAGTGGAGGCCATGATTACGAAGGCCTCTCCTATGTCTCCCAAATCGAAACACCTTTCCTTGTCGTAGACCTCGCAAAGCTTCGAACCATCAGTGTTGATATCAAATCCAACACTGCATGGGTTCAAGCCGGTGCCACCATTGGCGAACTCTACTACAGAATTGCAGAGAAAAGCAAGACTCTTGGCTTTCCTGCCGGACTCTGCACGAGTCTAGGCGTTGGTGGGCACATAACTGGTGGTGCATATGGCTCCATGTTGAGGAAATATGGCCTTGGTGCTGATAACGCTGTGGATGCTCGAATCATAGACTTTAACGGGAAGATTCTTGATCGAAAAGCAATGGGAGAGGATCTGTTTTGGGCAATTAGAGGAGGTGGAGGAGCAAGTTTCGGTATCATCCTTTGGTGGAAGGTAAAGTTGGTTCCAGTTCCAGCAACCGTGACTGGTTTTGCGGTTCCTAAGACCTTGGAACAAGGCGCCACAAAGCTCCTCTATAGATGGCAACAGGTAGCTCCACTGATTGATGATGCTCTCTTCATCAGAGTTCTCATCAGCAAAACTGGCAATACATCTGTCACAACTCTTTACCAAGCTCAATTTCTTGGAGGTGTTGATAGATTACTCAACGTCATGCAAAAAAGCTTCCCCGAATTGGGTTTGACACGAAAGGATTGTACCGAAATGAGCTGGATCAAATCAGTGATGTACATAGCAGGTTACCCAAGTACAACTCCTCCTGAAGTTTTGCTTCAAGGAAAATCCTCCATGCCCAAGGGCTACTTCAAAGCCAAATCAGACTTTGTCAAGACCCCGATTCCAGAAAAGGGGCTTGAGGGGCTGTGGAAGATGATACTACAAGAATCCTCTCCTCTGATGATTTTGAATCCGTATGGCGGAATGATGAGCAGGATTTCCGAAAGCGCAATAGCTTTTCCTCACAGAAATGTTCTCTTCAAAATTCAGTACGTGACTTTATGGCAAGCTGGAGATAACGAGGCACAGCATATGGATTGGATTAGGAAGCTTTACAATTACATGGGTTCTTATGTCACAAAGTTTCCAAGGCAGGCATATGTGAATTATAGGGATCTTGATTTGGGGACTAACAAGAAGAGTAGCTCAAGTTTCATTAAAGCAAGTTCTTGGGGTTACAGGTATTTCAAGGACAATTTTAATAGACTGGTTAAAATAAAGACCAAAGCCGATCCTCAAAACTTCTTCCGGCATGAGCAGAGCATCCCGCCTCTTCCACGAGGCCGTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000741 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006950 GO:0006979 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009559 GO:0009561 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010197 GO:0016043 GO:0016491 GO:0016614 GO:0016616 GO:0022414 GO:0030054 GO:0030154 GO:0030312 GO:0032501 GO:0032502 GO:0044464 GO:0045551 GO:0048229 GO:0048284 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050268 GO:0050896 GO:0055044 GO:0055114 GO:0061458 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

529

Amino Acids

59.17

Weight (kDa)

9.64

Isoelectric Point (pI)

33.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	78 - 216	9.5e-26	FAD binding domain
BBE	PF08031	464 - 521	1.2e-20	Berberine and berberine like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30760 AT1G30760 AT2G34790 AT5G44390 AT5G44400
fragaria_vesca	FvH4_3g10920
malus_domestica	MD05G1268400.v1.1 MD05G1268500.v1.1 MD10G1244500.v1.1
prunus_persica	Prupe.4G096300_v2.0.a1 Prupe.4G096400_v2.0.a1
pyrus_communis	pycom05g25130 pycom05g25140 pycom10g20500
rosa_chinensis	RchiOBHm_Chr5g0017561 RchiOBHm_Chr5g0017591 RchiOBHm_Chr5g0017601 RchiOBHm_Chr5g0017621 RchiOBHm_Chr5g0017641
rosa_laevigata	RLG00000032298 RLG00000032301 RLG00000032302
rosa_multiflora	Rmu_sc0000346.1_g000021 Rmu_sc0000346.1_g000022 Rmu_sc0002565.1_g000007 Rmu_sc0003415.1_g000020
rosa_roxburghii	Rroxscaffold_1G00059870 Rroxscaffold_1G00059880 Rroxscaffold_1G00059890
rosa_rugosa	Rorug05G0036900 Rorug05G0037000 Rorug05G0037100
rosa_wichuraiana	Rw5G011310 Rw5G011320 Rw5G011330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1025
AccB1I	GGYRCC	2 cut(s)	452, 797
AccI	GTMKAC	1 cut(s)	387
AccII	CGCG	1 cut(s)	290
AciI	CCGC	4 cut(s)	174, 773, 1215, 1562
AclWI	GGATC	4 cut(s)	678, 1019, 1416, 1520
AcsI	RAATTY	3 cut(s)	88, 160, 1278
AcuI	CTGAAG	2 cut(s)	123, 1080
AcyI	GRCGYC	1 cut(s)	798
AfaI	GTAC	5 cut(s)	209, 997, 1028, 1048, 1286
AfiI	CCNNNNNNNGG	7 cut(s)	211, 313, 579, 973, 974, 1145, 1214
AgeI	ACCGGT	1 cut(s)	250
AgsI	TTSAA	8 cut(s)	18, 78, 446, 1073, 1105, 1204, 1276, 1483
AjnI	CCWGG	1 cut(s)	56
AjuI	GAANNNNNNNTTGG	6 cut(s)	722, 754, 903, 935, 1085, 1117
AleI	CACNNNNGTG	1 cut(s)	251
Alw26I	GTCTC	2 cut(s)	220, 361
AlwI	GGATC	4 cut(s)	678, 1019, 1416, 1520
AoxI	GGCC	4 cut(s)	328, 343, 589, 1576
ApeKI	GCWGC	1 cut(s)	275
ApoI	RAATTY	3 cut(s)	88, 160, 1278
AsiGI	ACCGGT	1 cut(s)	250
AspLEI	GCGC	4 cut(s)	64, 290, 800, 1244
AspS9I	GGNCC	1 cut(s)	316
AsuHPI	GGTGA	1 cut(s)	163
AsuII	TTCGAA	2 cut(s)	301, 403
AvaII	GGWCC	1 cut(s)	316
BaeGI	GKGCMC	1 cut(s)	549
BanI	GGYRCC	2 cut(s)	452, 797
BauI	CACGAG	2 cut(s)	526, 1572
BbsI	GAAGAC	1 cut(s)	180
BbvI	GCAGC	1 cut(s)	262
BccI	CCATC	3 cut(s)	315, 416, 813
BceAI	ACGGC	1 cut(s)	1563
BciT130I	CCWGG	1 cut(s)	58
BcoDI	GTCTC	2 cut(s)	220, 361
BfaI	CTAG	3 cut(s)	165, 533, 1585
BfmI	CTRYAG	2 cut(s)	475, 814
BfoI	RGCGCY	1 cut(s)	801
BfuAI	ACCTGC	1 cut(s)	1025
BisI	GCNGC	1 cut(s)	276
BlsI	GCNGC	1 cut(s)	277
Bme1390I	CCNGG	1 cut(s)	58
Bme18I	GGWCC	1 cut(s)	316
BmgT120I	GGNCC	1 cut(s)	316
BmiI	GGNNCC	6 cut(s)	317, 454, 571, 744, 777, 799
BmrFI	CCNGG	1 cut(s)	58
BmsI	GCATC	4 cut(s)	73, 604, 838, 1566
BpiI	GAAGAC	1 cut(s)	180
BpmI	CTGGAG	1 cut(s)	1326
Bpu14I	TTCGAA	2 cut(s)	301, 403
BpuEI	CTTGAG	3 cut(s)	203, 1172, 1426
BsaAI	YACGTR	1 cut(s)	1288
BsaHI	GRCGYC	1 cut(s)	798
BsaI	GGTCTC	1 cut(s)	220
BsaJI	CCNNGG	5 cut(s)	57, 592, 786, 1092, 1382
BsaWI	WCCGGW	2 cut(s)	204, 250
BsaXI	ACNNNNNCTCC	6 cut(s)	186, 200, 216, 230, 1039, 1069
Bsc4I	CCNNNNNNNGG	7 cut(s)	211, 313, 579, 973, 974, 1145, 1214
Bse118I	RCCGGY	2 cut(s)	250, 449
Bse1I	ACTGG	5 cut(s)	559, 746, 769, 886, 1506
Bse3DI	GCAATG	1 cut(s)	666
BseBI	CCWGG	1 cut(s)	58
BseDI	CCNNGG	5 cut(s)	57, 592, 786, 1092, 1382
BseGI	GGATG	4 cut(s)	64, 619, 717, 1557
BseLI	CCNNNNNNNGG	7 cut(s)	211, 313, 579, 973, 974, 1145, 1214
BseMI	GCAATG	1 cut(s)	666
BseMII	CTCAG	2 cut(s)	234, 325
BseNI	ACTGG	5 cut(s)	559, 746, 769, 886, 1506
BseRI	GAGGAG	6 cut(s)	208, 705, 714, 800, 1044, 1179
BseSI	GKGCMC	1 cut(s)	549
BseXI	GCAGC	1 cut(s)	262
BsgI	GTGCAG	2 cut(s)	177, 508
Bsh1236I	CGCG	1 cut(s)	290
BshFI	GGCC	4 cut(s)	330, 345, 591, 1578
BshNI	GGYRCC	2 cut(s)	452, 797
BshTI	ACCGGT	1 cut(s)	250
BsiSI	CCGG	6 cut(s)	205, 233, 251, 450, 516, 1543
BslFI	GGGAC	1 cut(s)	1435
BslI	CCNNNNNNNGG	7 cut(s)	211, 313, 579, 973, 974, 1145, 1214
BsmAI	GTCTC	2 cut(s)	220, 361
BsmFI	GGGAC	1 cut(s)	1435
BsnI	GGCC	4 cut(s)	330, 345, 591, 1578
Bso31I	GGTCTC	1 cut(s)	220
Bsp119I	TTCGAA	2 cut(s)	301, 403
Bsp1286I	GDGCHC	1 cut(s)	549
Bsp1407I	TGTACA	1 cut(s)	1026
Bsp143I	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
BspACI	CCGC	4 cut(s)	174, 773, 1215, 1562
BspANI	GGCC	4 cut(s)	330, 345, 591, 1578
BspCNI	CTCAG	2 cut(s)	233, 324
BspFNI	CGCG	1 cut(s)	290
BspLI	GGNNCC	6 cut(s)	317, 454, 571, 744, 777, 799
BspMI	ACCTGC	1 cut(s)	1025
BspPI	GGATC	4 cut(s)	678, 1019, 1416, 1520
BspT104I	TTCGAA	2 cut(s)	301, 403
BspT107I	GGYRCC	2 cut(s)	452, 797
BspTNI	GGTCTC	1 cut(s)	220
BsrDI	GCAATG	1 cut(s)	666
BsrFI	RCCGGY	2 cut(s)	250, 449
BsrGI	TGTACA	1 cut(s)	1026
BsrI	ACTGG	5 cut(s)	559, 746, 769, 886, 1506
BssAI	RCCGGY	2 cut(s)	250, 449
BssECI	CCNNGG	5 cut(s)	57, 592, 786, 1092, 1382
BssMI	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
BssNI	GRCGYC	1 cut(s)	798
BssSI	CACGAG	2 cut(s)	526, 1572
BssT1I	CCWWGG	4 cut(s)	592, 786, 1092, 1382
Bst2BI	CACGAG	2 cut(s)	526, 1572
Bst2UI	CCWGG	1 cut(s)	58
Bst4CI	ACNGT	1 cut(s)	760
Bst6I	CTCTTC	5 cut(s)	142, 860, 1277, 1427, 1572
BstACI	GRCGYC	1 cut(s)	798
BstAUI	TGTACA	1 cut(s)	1026
BstBAI	YACGTR	1 cut(s)	1288
BstBI	TTCGAA	2 cut(s)	301, 403
BstC8I	GCNNGC	3 cut(s)	273, 1302, 1389
BstDEI	CTNAG	4 cut(s)	117, 220, 311, 780
BstEII	GGTNACC	1 cut(s)	1037
BstF5I	GGATG	4 cut(s)	64, 619, 717, 1557
BstFNI	CGCG	1 cut(s)	290
BstH2I	RGCGCY	1 cut(s)	801
BstHHI	GCGC	4 cut(s)	64, 290, 800, 1244
BstKTI	GATC	5 cut(s)	652, 673, 1014, 1411, 1528
BstMAI	GTCTC	2 cut(s)	220, 361
BstMBI	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
BstMWI	GCNNNNNNNGC	5 cut(s)	59, 605, 614, 882, 1156
BstNI	CCWGG	1 cut(s)	58
BstPI	GGTNACC	1 cut(s)	1037
BstSCI	CCNGG	1 cut(s)	56
BstSFI	CTRYAG	2 cut(s)	475, 814
BstSLI	GKGCMC	1 cut(s)	549
BstUI	CGCG	1 cut(s)	290
BstV1I	GCAGC	1 cut(s)	262
BstV2I	GAAGAC	1 cut(s)	180
BstX2I	RGATCY	2 cut(s)	670, 1408
BstYI	RGATCY	2 cut(s)	670, 1408
BsuRI	GGCC	4 cut(s)	330, 345, 591, 1578
BtsCI	GGATG	4 cut(s)	64, 619, 717, 1557
BtsI	GCAGTG	1 cut(s)	432
BtsIMutI	CAGTG	5 cut(s)	228, 418, 432, 836, 1026
BveI	ACCTGC	1 cut(s)	1025
Cac8I	GCNNGC	3 cut(s)	273, 1302, 1389
CfoI	GCGC	4 cut(s)	64, 290, 800, 1244
Cfr10I	RCCGGY	2 cut(s)	250, 449
Cfr13I	GGNCC	1 cut(s)	316
Csp6I	GTAC	5 cut(s)	208, 996, 1027, 1047, 1285
CspAI	ACCGGT	1 cut(s)	250
CviAII	CATG	9 cut(s)	257, 266, 332, 438, 574, 952, 1087, 1357, 1547
CviQI	GTAC	5 cut(s)	208, 996, 1027, 1047, 1285
DdeI	CTNAG	4 cut(s)	117, 220, 311, 780
DinI	GGCGCC	1 cut(s)	799
DpnI	GATC	5 cut(s)	651, 672, 1013, 1410, 1527
DpnII	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
Eam1104I	CTCTTC	5 cut(s)	142, 860, 1277, 1427, 1572
EarI	CTCTTC	5 cut(s)	142, 860, 1277, 1427, 1572
EciI	GGCGGA	1 cut(s)	1230
Eco130I	CCWWGG	4 cut(s)	592, 786, 1092, 1382
Eco147I	AGGCCT	1 cut(s)	345
Eco31I	GGTCTC	1 cut(s)	220
Eco32I	GATATC	1 cut(s)	421
Eco47I	GGWCC	1 cut(s)	316
Eco57I	CTGAAG	2 cut(s)	123, 1080
Eco91I	GGTNACC	1 cut(s)	1037
EcoO65I	GGTNACC	1 cut(s)	1037
EcoRII	CCWGG	1 cut(s)	56
EcoRV	GATATC	1 cut(s)	421
EcoT14I	CCWWGG	4 cut(s)	592, 786, 1092, 1382
EgeI	GGCGCC	1 cut(s)	799
EheI	GGCGCC	1 cut(s)	799
ErhI	CCWWGG	4 cut(s)	592, 786, 1092, 1382
FaeI	CATG	9 cut(s)	260, 269, 335, 441, 577, 955, 1090, 1360, 1550
FalI	AAGNNNNNCTT	4 cut(s)	1053, 1085, 1085, 1117
FaqI	GGGAC	1 cut(s)	1435
FatI	CATG	9 cut(s)	256, 265, 331, 437, 573, 951, 1086, 1356, 1546
FauI	CCCGC	1 cut(s)	1569
FauNDI	CATATG	3 cut(s)	565, 1326, 1393
FblI	GTMKAC	1 cut(s)	387
Fnu4HI	GCNGC	1 cut(s)	276
FokI	GGATG	4 cut(s)	51, 626, 704, 1544
Fsp4HI	GCNGC	1 cut(s)	276
FspBI	CTAG	3 cut(s)	165, 533, 1585
GlaI	GCGC	4 cut(s)	63, 289, 799, 1243
GluI	GCNGC	1 cut(s)	276
GsuI	CTGGAG	1 cut(s)	1326
HaeII	RGCGCY	1 cut(s)	801
HaeIII	GGCC	4 cut(s)	330, 345, 591, 1578
HapII	CCGG	6 cut(s)	205, 233, 251, 450, 516, 1543
HhaI	GCGC	4 cut(s)	64, 290, 800, 1244
Hin1I	GRCGYC	1 cut(s)	798
Hin1II	CATG	9 cut(s)	260, 269, 335, 441, 577, 955, 1090, 1360, 1550
Hin6I	GCGC	4 cut(s)	62, 288, 798, 1242
HinP1I	GCGC	4 cut(s)	62, 288, 798, 1242
HindIII	AAGCTT	4 cut(s)	296, 398, 961, 1343
HpaII	CCGG	6 cut(s)	205, 233, 251, 450, 516, 1543
HphI	GGTGA	1 cut(s)	163
Hpy166II	GTNNAC	1 cut(s)	388
Hpy188I	TCNGA	7 cut(s)	142, 156, 320, 863, 1117, 1194, 1237
Hpy188III	TCNNGA	6 cut(s)	182, 647, 1058, 1126, 1139, 1412
Hpy8I	GTNNAC	1 cut(s)	388
HpyAV	CCTTC	3 cut(s)	84, 335, 724
HpyCH4III	ACNGT	1 cut(s)	760
HpyCH4IV	ACGT	3 cut(s)	109, 948, 1287
HpyCH4V	TGCA	8 cut(s)	194, 256, 271, 437, 485, 525, 563, 955
HpyF10VI	GCNNNNNNNGC	5 cut(s)	59, 605, 614, 882, 1156
HpyF3I	CTNAG	4 cut(s)	117, 220, 311, 780
HpySE526I	ACGT	3 cut(s)	109, 948, 1287
Hsp92I	GRCGYC	1 cut(s)	798
Hsp92II	CATG	9 cut(s)	260, 269, 335, 441, 577, 955, 1090, 1360, 1550
HspAI	GCGC	4 cut(s)	62, 288, 798, 1242
KasI	GGCGCC	1 cut(s)	797
Kzo9I	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
LmnI	GCTCC	4 cut(s)	575, 701, 813, 838
Lsp1109I	GCAGC	1 cut(s)	262
LweI	GCATC	4 cut(s)	73, 604, 838, 1566
MaeI	CTAG	3 cut(s)	165, 533, 1585
MaeII	ACGT	3 cut(s)	109, 948, 1287
MaeIII	GTNAC	7 cut(s)	760, 895, 1037, 1288, 1369, 1469, 1580
MalI	GATC	5 cut(s)	651, 672, 1013, 1410, 1527
MboI	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
MboII	GAAGA	9 cut(s)	129, 180, 652, 847, 1177, 1264, 1444, 1531, 1559
MflI	RGATCY	2 cut(s)	670, 1408
MhlI	GDGCHC	1 cut(s)	549
Mly113I	GGCGCC	1 cut(s)	798
MlyI	GAGTC	5 cut(s)	115, 194, 493, 513, 538
MmeI	TCCRAC	1 cut(s)	453
MseI	TTAA	4 cut(s)	633, 1452, 1494, 1506
MslI	CAYNNNNRTG	2 cut(s)	251, 1263
MspA1I	CMGCKG	1 cut(s)	176
MspI	CCGG	6 cut(s)	205, 233, 251, 450, 516, 1543
MspR9I	CCNGG	1 cut(s)	58
MvaI	CCWGG	1 cut(s)	58
MvnI	CGCG	1 cut(s)	290
MwoI	GCNNNNNNNGC	5 cut(s)	59, 605, 614, 882, 1156
NarI	GGCGCC	1 cut(s)	798
NdeI	CATATG	3 cut(s)	565, 1326, 1393
NdeII	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
NlaIII	CATG	9 cut(s)	260, 269, 335, 441, 577, 955, 1090, 1360, 1550
NlaIV	GGNNCC	6 cut(s)	317, 454, 571, 744, 777, 799
NmuCI	GTSAC	4 cut(s)	760, 895, 1288, 1369
NspV	TTCGAA	2 cut(s)	301, 403
OliI	CACNNNNGTG	1 cut(s)	251
PceI	AGGCCT	1 cut(s)	345
PfeI	GAWTC	7 cut(s)	260, 303, 621, 643, 1135, 1181, 1204
PflFI	GACNNNGTC	1 cut(s)	1121
PinAI	ACCGGT	1 cut(s)	250
PkrI	GCNGC	1 cut(s)	277
PleI	GAGTC	5 cut(s)	115, 193, 493, 513, 537
PluTI	GGCGCC	1 cut(s)	801
PpsI	GAGTC	5 cut(s)	115, 193, 493, 513, 537
Ppu21I	YACGTR	1 cut(s)	1288
Psp6I	CCWGG	1 cut(s)	56
PspEI	GGTNACC	1 cut(s)	1037
PspGI	CCWGG	1 cut(s)	56
PspN4I	GGNNCC	6 cut(s)	317, 454, 571, 744, 777, 799
PspPI	GGNCC	1 cut(s)	316
PsrI	GAACNNNNNNTAC	2 cut(s)	726, 758
PsuI	RGATCY	2 cut(s)	670, 1408
PsyI	GACNNNGTC	1 cut(s)	1121
RsaI	GTAC	5 cut(s)	209, 997, 1028, 1048, 1286
RsaNI	GTAC	5 cut(s)	208, 996, 1027, 1047, 1285
RseI	CAYNNNNRTG	2 cut(s)	251, 1263
SaqAI	TTAA	4 cut(s)	633, 1452, 1494, 1506
SatI	GCNGC	1 cut(s)	276
Sau3AI	GATC	5 cut(s)	649, 670, 1011, 1408, 1525
Sau96I	GGNCC	1 cut(s)	316
SchI	GAGTC	5 cut(s)	115, 194, 493, 513, 538
ScrFI	CCNGG	1 cut(s)	58
SduI	GDGCHC	1 cut(s)	549
SfaNI	GCATC	4 cut(s)	73, 604, 838, 1566
SfcI	CTRYAG	2 cut(s)	475, 814
SfoI	GGCGCC	1 cut(s)	799
SfuI	TTCGAA	2 cut(s)	301, 403
SgrAI	CRCCGGYG	1 cut(s)	250
SinI	GGWCC	1 cut(s)	316
SmiMI	CAYNNNNRTG	2 cut(s)	251, 1263
SmlI	CTYRAG	3 cut(s)	182, 1151, 1441
SmoI	CTYRAG	3 cut(s)	182, 1151, 1441
SseBI	AGGCCT	1 cut(s)	345
SsiI	CCGC	4 cut(s)	174, 773, 1215, 1562
SspDI	GGCGCC	1 cut(s)	797
SspMI	CTAG	3 cut(s)	165, 533, 1585
StuI	AGGCCT	1 cut(s)	345
StyD4I	CCNGG	1 cut(s)	56
StyI	CCWWGG	4 cut(s)	592, 786, 1092, 1382
TaaI	ACNGT	1 cut(s)	760
TaiI	ACGT	3 cut(s)	112, 951, 1290
TaqI	TCGA	5 cut(s)	301, 366, 403, 619, 652
TatI	WGTACW	2 cut(s)	1026, 1046
TfiI	GAWTC	7 cut(s)	260, 303, 621, 643, 1135, 1181, 1204
Tru1I	TTAA	4 cut(s)	633, 1452, 1494, 1506
Tru9I	TTAA	4 cut(s)	633, 1452, 1494, 1506
TscAI	CASTG	5 cut(s)	228, 418, 439, 843, 1026
TseFI	GTSAC	4 cut(s)	760, 895, 1288, 1369
TseI	GCWGC	1 cut(s)	275
Tsp45I	GTSAC	4 cut(s)	760, 895, 1288, 1369
TspDTI	ATGAA	2 cut(s)	847, 1438
TspGWI	ACGGA	1 cut(s)	1197
TspRI	CASTG	5 cut(s)	228, 418, 439, 843, 1026
Tth111I	GACNNNGTC	1 cut(s)	1121
VpaK11BI	GGWCC	1 cut(s)	316
XapI	RAATTY	3 cut(s)	88, 160, 1278
XmiI	GTMKAC	1 cut(s)	387
XspI	CTAG	3 cut(s)	165, 533, 1585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.